José Guilherme Datorre, Mariana Bisarro Dos Reis, Bruna Pereira Sorroche, Gustavo Ramos Teixeira, Silveli Suzuki Hatano, Ana Carolina de Carvalho, Ricardo Ribeiro Gama, Lidia Maria Rebolho Batista Arantes, Rui Manuel Reis
{"title":"头颈部癌症患者瘤内核分枝杆菌与癌症特异性生存率的提高有关。","authors":"José Guilherme Datorre, Mariana Bisarro Dos Reis, Bruna Pereira Sorroche, Gustavo Ramos Teixeira, Silveli Suzuki Hatano, Ana Carolina de Carvalho, Ricardo Ribeiro Gama, Lidia Maria Rebolho Batista Arantes, Rui Manuel Reis","doi":"10.1080/20002297.2025.2487644","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>The oral microbiome, particularly <i>Fusobacterium nucleatum (Fn)</i>, has been implicated in head and neck cancers (HNC), influencing local immunity and Human Papillomavirus (HPV) status. Here, we evaluated the presence of <i>Fn</i> and its association with HPV infection, <i>TERT</i> promoter (<i>TERTp</i>) mutations, and patient outcomes.</p><p><strong>Materials and methods: </strong>We analyzed 94 formalin-fixed paraffin-embedded (FFPE) tumor tissues from HNC patients previously evaluated for <i>TERTp</i> mutations. <i>Fn</i> DNA was detected using droplet digital PCR (ddPCR), and HPV status was determined via p16 immunohistochemistry in pre-treatment samples. Associations between <i>Fn</i> presence, clinicopathological features, HPV, and TERTp mutation status were assessed.</p><p><strong>Results: </strong>Tumors primarily originated from the oropharynx (70.2%) and oral cavity (29.8%). Tobacco and alcohol use were reported in 87.2% and 79.8% of cases, respectively. <i>Fn</i> was present in 59.6% of cases, with higher prevalence in oropharyngeal (62.1%) than oral cavity (53.6%) tumors. No significant associations were found between <i>Fn</i> and clinicopathological features, TERTp, or HPV status. However, patients with <i>Fn</i> positivity showed significantly improved cancer-specific survival (61.5% vs. 39.1%, <i>p</i> = 0.013), similar to HPV-positive patients (72.7% vs. 42.7%, <i>p</i> = 0.014).</p><p><strong>Conclusion: </strong>The presence of <i>Fusobacterium nucleatum</i> in HNC correlates with longer survival, highlighting its potential as a prognostic marker.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"17 1","pages":"2487644"},"PeriodicalIF":3.7000,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11966973/pdf/","citationCount":"0","resultStr":"{\"title\":\"Intratumoral <i>Fusobacterium nucleatum</i> is associated with better cancer-specific survival in head and neck cancer patients.\",\"authors\":\"José Guilherme Datorre, Mariana Bisarro Dos Reis, Bruna Pereira Sorroche, Gustavo Ramos Teixeira, Silveli Suzuki Hatano, Ana Carolina de Carvalho, Ricardo Ribeiro Gama, Lidia Maria Rebolho Batista Arantes, Rui Manuel Reis\",\"doi\":\"10.1080/20002297.2025.2487644\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>The oral microbiome, particularly <i>Fusobacterium nucleatum (Fn)</i>, has been implicated in head and neck cancers (HNC), influencing local immunity and Human Papillomavirus (HPV) status. Here, we evaluated the presence of <i>Fn</i> and its association with HPV infection, <i>TERT</i> promoter (<i>TERTp</i>) mutations, and patient outcomes.</p><p><strong>Materials and methods: </strong>We analyzed 94 formalin-fixed paraffin-embedded (FFPE) tumor tissues from HNC patients previously evaluated for <i>TERTp</i> mutations. <i>Fn</i> DNA was detected using droplet digital PCR (ddPCR), and HPV status was determined via p16 immunohistochemistry in pre-treatment samples. Associations between <i>Fn</i> presence, clinicopathological features, HPV, and TERTp mutation status were assessed.</p><p><strong>Results: </strong>Tumors primarily originated from the oropharynx (70.2%) and oral cavity (29.8%). Tobacco and alcohol use were reported in 87.2% and 79.8% of cases, respectively. <i>Fn</i> was present in 59.6% of cases, with higher prevalence in oropharyngeal (62.1%) than oral cavity (53.6%) tumors. No significant associations were found between <i>Fn</i> and clinicopathological features, TERTp, or HPV status. However, patients with <i>Fn</i> positivity showed significantly improved cancer-specific survival (61.5% vs. 39.1%, <i>p</i> = 0.013), similar to HPV-positive patients (72.7% vs. 42.7%, <i>p</i> = 0.014).</p><p><strong>Conclusion: </strong>The presence of <i>Fusobacterium nucleatum</i> in HNC correlates with longer survival, highlighting its potential as a prognostic marker.</p>\",\"PeriodicalId\":16598,\"journal\":{\"name\":\"Journal of Oral Microbiology\",\"volume\":\"17 1\",\"pages\":\"2487644\"},\"PeriodicalIF\":3.7000,\"publicationDate\":\"2025-04-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11966973/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Oral Microbiology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1080/20002297.2025.2487644\",\"RegionNum\":2,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q2\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Oral Microbiology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1080/20002297.2025.2487644","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
Intratumoral Fusobacterium nucleatum is associated with better cancer-specific survival in head and neck cancer patients.
Background: The oral microbiome, particularly Fusobacterium nucleatum (Fn), has been implicated in head and neck cancers (HNC), influencing local immunity and Human Papillomavirus (HPV) status. Here, we evaluated the presence of Fn and its association with HPV infection, TERT promoter (TERTp) mutations, and patient outcomes.
Materials and methods: We analyzed 94 formalin-fixed paraffin-embedded (FFPE) tumor tissues from HNC patients previously evaluated for TERTp mutations. Fn DNA was detected using droplet digital PCR (ddPCR), and HPV status was determined via p16 immunohistochemistry in pre-treatment samples. Associations between Fn presence, clinicopathological features, HPV, and TERTp mutation status were assessed.
Results: Tumors primarily originated from the oropharynx (70.2%) and oral cavity (29.8%). Tobacco and alcohol use were reported in 87.2% and 79.8% of cases, respectively. Fn was present in 59.6% of cases, with higher prevalence in oropharyngeal (62.1%) than oral cavity (53.6%) tumors. No significant associations were found between Fn and clinicopathological features, TERTp, or HPV status. However, patients with Fn positivity showed significantly improved cancer-specific survival (61.5% vs. 39.1%, p = 0.013), similar to HPV-positive patients (72.7% vs. 42.7%, p = 0.014).
Conclusion: The presence of Fusobacterium nucleatum in HNC correlates with longer survival, highlighting its potential as a prognostic marker.
期刊介绍:
As the first Open Access journal in its field, the Journal of Oral Microbiology aims to be an influential source of knowledge on the aetiological agents behind oral infectious diseases. The journal is an international forum for original research on all aspects of ''oral health''. Articles which seek to understand ''oral health'' through exploration of the pathogenesis, virulence, host-parasite interactions, and immunology of oral infections are of particular interest. However, the journal also welcomes work that addresses the global agenda of oral infectious diseases and articles that present new strategies for treatment and prevention or improvements to existing strategies.
Topics: ''oral health'', microbiome, genomics, host-pathogen interactions, oral infections, aetiologic agents, pathogenesis, molecular microbiology systemic diseases, ecology/environmental microbiology, treatment, diagnostics, epidemiology, basic oral microbiology, and taxonomy/systematics.
Article types: original articles, notes, review articles, mini-reviews and commentaries