头颈部癌症患者瘤内核分枝杆菌与癌症特异性生存率的提高有关。

IF 3.7 2区 医学 Q2 MICROBIOLOGY
Journal of Oral Microbiology Pub Date : 2025-04-01 eCollection Date: 2025-01-01 DOI:10.1080/20002297.2025.2487644
José Guilherme Datorre, Mariana Bisarro Dos Reis, Bruna Pereira Sorroche, Gustavo Ramos Teixeira, Silveli Suzuki Hatano, Ana Carolina de Carvalho, Ricardo Ribeiro Gama, Lidia Maria Rebolho Batista Arantes, Rui Manuel Reis
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引用次数: 0

摘要

背景:口腔微生物群,特别是核梭杆菌(Fn),与头颈癌(HNC)有关,影响局部免疫和人乳头瘤病毒(HPV)状态。在这里,我们评估了Fn的存在及其与HPV感染、TERT启动子(TERTp)突变和患者预后的关系。材料和方法:我们分析了94例福尔马林固定石蜡包埋(FFPE)肿瘤组织,这些组织来自HNC患者,此前曾评估过TERTp突变。采用微滴数字PCR (ddPCR)检测Fn DNA,通过p16免疫组化检测预处理样品的HPV状态。评估Fn存在、临床病理特征、HPV和TERTp突变状态之间的关系。结果:肿瘤主要来源于口咽部(70.2%)和口腔(29.8%)。吸烟和饮酒分别占病例的87.2%和79.8%。Fn在59.6%的病例中存在,口咽肿瘤(62.1%)高于口腔肿瘤(53.6%)。Fn与临床病理特征、TERTp或HPV状态之间未发现显著关联。然而,Fn阳性患者的癌症特异性生存率显著提高(61.5%比39.1%,p = 0.013),与hpv阳性患者相似(72.7%比42.7%,p = 0.014)。结论:核梭杆菌在HNC中的存在与更长的生存期相关,突出了其作为预后标志物的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Intratumoral Fusobacterium nucleatum is associated with better cancer-specific survival in head and neck cancer patients.

Background: The oral microbiome, particularly Fusobacterium nucleatum (Fn), has been implicated in head and neck cancers (HNC), influencing local immunity and Human Papillomavirus (HPV) status. Here, we evaluated the presence of Fn and its association with HPV infection, TERT promoter (TERTp) mutations, and patient outcomes.

Materials and methods: We analyzed 94 formalin-fixed paraffin-embedded (FFPE) tumor tissues from HNC patients previously evaluated for TERTp mutations. Fn DNA was detected using droplet digital PCR (ddPCR), and HPV status was determined via p16 immunohistochemistry in pre-treatment samples. Associations between Fn presence, clinicopathological features, HPV, and TERTp mutation status were assessed.

Results: Tumors primarily originated from the oropharynx (70.2%) and oral cavity (29.8%). Tobacco and alcohol use were reported in 87.2% and 79.8% of cases, respectively. Fn was present in 59.6% of cases, with higher prevalence in oropharyngeal (62.1%) than oral cavity (53.6%) tumors. No significant associations were found between Fn and clinicopathological features, TERTp, or HPV status. However, patients with Fn positivity showed significantly improved cancer-specific survival (61.5% vs. 39.1%, p = 0.013), similar to HPV-positive patients (72.7% vs. 42.7%, p = 0.014).

Conclusion: The presence of Fusobacterium nucleatum in HNC correlates with longer survival, highlighting its potential as a prognostic marker.

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来源期刊
CiteScore
8.00
自引率
4.40%
发文量
52
审稿时长
12 weeks
期刊介绍: As the first Open Access journal in its field, the Journal of Oral Microbiology aims to be an influential source of knowledge on the aetiological agents behind oral infectious diseases. The journal is an international forum for original research on all aspects of ''oral health''. Articles which seek to understand ''oral health'' through exploration of the pathogenesis, virulence, host-parasite interactions, and immunology of oral infections are of particular interest. However, the journal also welcomes work that addresses the global agenda of oral infectious diseases and articles that present new strategies for treatment and prevention or improvements to existing strategies. Topics: ''oral health'', microbiome, genomics, host-pathogen interactions, oral infections, aetiologic agents, pathogenesis, molecular microbiology systemic diseases, ecology/environmental microbiology, treatment, diagnostics, epidemiology, basic oral microbiology, and taxonomy/systematics. Article types: original articles, notes, review articles, mini-reviews and commentaries
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