Kirill V Larin, Irina V Larina, Michael Liebling, Mary E Dickinson
{"title":"Live Imaging of Early Developmental Processes in Mammalian Embryos with Optical Coherence Tomography.","authors":"Kirill V Larin, Irina V Larina, Michael Liebling, Mary E Dickinson","doi":"10.1142/S1793545809000619","DOIUrl":"10.1142/S1793545809000619","url":null,"abstract":"<p><p>Early embryonic imaging of cardiovascular development in mammalian models requires a method that can penetrate through and distinguish the many tissue layers with high spatial and temporal resolution. In this paper we evaluate the capability of Optical Coherence Tomography (OCT) technique for structural 3D embryonic imaging in mouse embryos at different stages of the developmental process ranging from 7.5 dpc up to 10.5 dpc. Obtained results suggest that the collected data is suitable for quantitative and qualitative measurements to assess cardiovascular function in mouse models, which is likely to expand our knowledge of the complexity of the embryonic heart, and its development into an adult heart.</p>","PeriodicalId":16248,"journal":{"name":"Journal of Innovative Optical Health Sciences","volume":"2 3","pages":"253-259"},"PeriodicalIF":2.3,"publicationDate":"2009-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2891056/pdf/nihms203889.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29082649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Adam M Larson, Anthony Lee, Po-Feng Lee, Kayla J Bayless, Alvin T Yeh
{"title":"ULTRASHORT PULSE MULTISPECTRAL NONLINEAR OPTICAL MICROSCOPY.","authors":"Adam M Larson, Anthony Lee, Po-Feng Lee, Kayla J Bayless, Alvin T Yeh","doi":"10.1142/S1793545809000292","DOIUrl":"10.1142/S1793545809000292","url":null,"abstract":"<p><p>Ultrashort pulse, multispectral nonlinear optical microscopy (NLOM) is developed and used to image, simultaneously, a mixed population of cells expressing different fluorescent protein mutants in a 3D tissue model of angiogenesis. Broadband, sub-10-fs pulses are used to excite multiple fluorescent proteins and generate second harmonic in collagen simultaneously. A 16-channel multispectral detector is used to delineate the multiple nonlinear optical signals, pixel by pixel, in NLOM. The ability to image multiple fluorescent protein mutants and collagen, simultaneously, enables serial measurements of cell-cell and cell-matrix interactions in our 3D tissue model and characterization of fundamental processes in angiogenic morphogenesis.</p>","PeriodicalId":16248,"journal":{"name":"Journal of Innovative Optical Health Sciences","volume":"2 1","pages":"27-35"},"PeriodicalIF":2.3,"publicationDate":"2009-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2773561/pdf/nihms146927.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28496282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Li Li, Hao F Zhang, Roger J Zemp, Konstantin Maslov, Lihong Wang
{"title":"Simultaneous imaging of a lacZ-marked tumor and microvasculature morphology in vivo by dual-wavelength photoacoustic microscopy.","authors":"Li Li, Hao F Zhang, Roger J Zemp, Konstantin Maslov, Lihong Wang","doi":"10.1142/S1793545808000212","DOIUrl":"10.1142/S1793545808000212","url":null,"abstract":"<p><p>Photoacoustic molecular imaging, combined with the reporter-gene technique, can provide a valuable tool for cancer research. The expression of the lacZ reporter gene can be imaged using photoacoustic imaging following the injection of X-gal, a colorimetric assay for the lacZ-encoded enzyme β-galactosidase. Dual-wavelength photoacoustic microscopy was used to non-invasively image the detailed morphology of a lacZ-marked 9L gliosarcoma and its surrounding microvasculature simultaneously in vivo, with a superior resolution on the order of 10 μm. Tumor-feeding vessels were found, and the expression level of lacZ in tumor was estimated. With future development of new absorption-enhancing reporter-gene systems, we anticipate this strategy can lead to a better understanding of the role of tumor metabolism in cancer initiation, progression, and metastasis, and in its response to therapy.</p>","PeriodicalId":16248,"journal":{"name":"Journal of Innovative Optical Health Sciences","volume":"1 2","pages":"207-215"},"PeriodicalIF":2.3,"publicationDate":"2008-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2782593/pdf/nihms91188.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28535715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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