超短脉冲多光谱非线性光学显微镜。

IF 2.3 3区医学 Q2 OPTICS

Journal of Innovative Optical Health Sciences Pub Date : 2009-01-01 DOI:10.1142/S1793545809000292

Adam M Larson, Anthony Lee, Po-Feng Lee, Kayla J Bayless, Alvin T Yeh

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引用次数: 4

摘要

超短脉冲，多光谱非线性光学显微镜(NLOM)被开发并用于成像，同时，在血管生成的三维组织模型中表达不同荧光蛋白突变的混合细胞群。宽带，低于10-fs脉冲用于激发多种荧光蛋白，并同时在胶原蛋白中产生二次谐波。在NLOM中，采用16通道多光谱检测器对多个非线性光信号进行逐像素描绘。同时成像多种荧光蛋白突变体和胶原蛋白的能力，使我们能够在3D组织模型中连续测量细胞-细胞和细胞-基质相互作用，并表征血管生成形态发生的基本过程。

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ULTRASHORT PULSE MULTISPECTRAL NONLINEAR OPTICAL MICROSCOPY.

Ultrashort pulse, multispectral nonlinear optical microscopy (NLOM) is developed and used to image, simultaneously, a mixed population of cells expressing different fluorescent protein mutants in a 3D tissue model of angiogenesis. Broadband, sub-10-fs pulses are used to excite multiple fluorescent proteins and generate second harmonic in collagen simultaneously. A 16-channel multispectral detector is used to delineate the multiple nonlinear optical signals, pixel by pixel, in NLOM. The ability to image multiple fluorescent protein mutants and collagen, simultaneously, enables serial measurements of cell-cell and cell-matrix interactions in our 3D tissue model and characterization of fundamental processes in angiogenic morphogenesis.

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来源期刊

Journal of Innovative Optical Health Sciences OPTICS-RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING

CiteScore

4.50

自引率

20.00%

发文量

审稿时长

>12 weeks

期刊介绍： JIOHS serves as an international forum for the publication of the latest developments in all areas of photonics in biology and medicine. JIOHS will consider for publication original papers in all disciplines of photonics in biology and medicine, including but not limited to: -Photonic therapeutics and diagnostics- Optical clinical technologies and systems- Tissue optics- Laser-tissue interaction and tissue engineering- Biomedical spectroscopy- Advanced microscopy and imaging- Nanobiophotonics and optical molecular imaging- Multimodal and hybrid biomedical imaging- Micro/nanofabrication- Medical microsystems- Optical coherence tomography- Photodynamic therapy. JIOHS provides a vehicle to help professionals, graduates, engineers, academics and researchers working in the field of intelligent photonics in biology and medicine to disseminate information on the state-of-the-art technique.