Journal of Innovative Optical Health Sciences最新文献_第6页

PARAMETRIC STUDY OF TISSUE OPTICAL CLEARING BY LOCALIZED MECHANICAL COMPRESSION USING COMBINED FINITE ELEMENT AND MONTE CARLO SIMULATION. 用有限元和蒙特卡罗模拟相结合的方法研究局部机械压缩组织光学清除的参数化。

IF 2.5 3区医学

Journal of Innovative Optical Health Sciences Pub Date : 2010-07-01 DOI: 10.1142/S179354581000099X

William C Vogt, Haiou Shen, Ge Wang, Christopher G Rylander

引用次数: 13

Photoacoustic generation of focused quasi-unipolar pressure pulses. 聚焦准单极压力脉冲的光声产生。

IF 2.5 3区医学

Journal of Innovative Optical Health Sciences Pub Date : 2010-01-01 DOI: 10.1142/S1793545810001118

Konstantin Maslov, Hao F Zhang, Lihong V Wang

引用次数: 4

ASSESSMENT OF TISSUE OPTICAL CLEARING AS A FUNCTION OF GLUCOSE CONCENTRATION USING OPTICAL COHERENCE TOMOGRAPHY. 利用光学相干断层成像评估组织光学清晰度作为葡萄糖浓度的函数。

IF 2.5 3区医学

Journal of Innovative Optical Health Sciences Pub Date : 2010-01-01 DOI: 10.1142/S1793545810001039

Narendran Sudheendran, Mohamed Mohamed, Mohamad G Ghosn, Valery V Tuchin, Kirill V Larin

{"title":"ASSESSMENT OF TISSUE OPTICAL CLEARING AS A FUNCTION OF GLUCOSE CONCENTRATION USING OPTICAL COHERENCE TOMOGRAPHY.","authors":"Narendran Sudheendran, Mohamed Mohamed, Mohamad G Ghosn, Valery V Tuchin, Kirill V Larin","doi":"10.1142/S1793545810001039","DOIUrl":"https://doi.org/10.1142/S1793545810001039","url":null,"abstract":"One of the major challenges in imaging biological tissues using optical techniques, such as optical coherence tomography (OCT), is the lack of light penetration due to highly turbid structures within the tissue. Optical clearing techniques enable the biological samples to be more optically homogeneous, allowing for deeper penetration of light into the tissue. This study investigates the effect of optical clearing utilizing various concentrations of glucose solution (10%, 30%, and 50%) on porcine skin. A gold-plated mirror was imaged beneath the tissue and percentage clearing was determined by monitoring the change in reflected light intensity from the mirror over time. The ratio of percentage clearing per tissue thickness for 10%, 30% and 50% glucose was determined to be 4.7 ± 1.6% mm(-1) (n = 6), 10.6 ± 2.0% mm(-1) (n = 7) and 21.8 ± 2.2% mm(-1) (n = 5), respectively. It was concluded that while higher glucose concentration has the highest optical clearing effect, a suitable concentration should be chosen for the purpose of clearing, considering the osmotic stress on the tissue sample.","PeriodicalId":16248,"journal":{"name":"Journal of Innovative Optical Health Sciences","volume":"3 3","pages":"169-176"},"PeriodicalIF":2.5,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1142/S1793545810001039","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30259050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 30

QUANTITATIVE REDOX SCANNING OF TISSUE SAMPLES USING A CALIBRATION PROCEDURE. 使用校准程序对组织样品进行定量氧化还原扫描。

IF 2.5 3区医学

Journal of Innovative Optical Health Sciences Pub Date : 2009-10-01 DOI: 10.1142/S1793545809000681

He N Xu, Baohua Wu, Shoko Nioka, Britton Chance, Lin Z Li

{"title":"QUANTITATIVE REDOX SCANNING OF TISSUE SAMPLES USING A CALIBRATION PROCEDURE.","authors":"He N Xu, Baohua Wu, Shoko Nioka, Britton Chance, Lin Z Li","doi":"10.1142/S1793545809000681","DOIUrl":"10.1142/S1793545809000681","url":null,"abstract":"The fluorescence properties of reduced nicotinamide adenine dinucleotide (NADH) and oxidized flavoproteins (Fp) including flavin adenine dinucleotide (FAD) in the respiratory chain are sensitive indicators of intracellular metabolic states and have been applied to the studies of mitochondrial function with energy-linked processes. The redox scanner, a three-dimensional (3D) low temperature imager previously developed by Chance et al., measures the in vivo metabolic properties of tissue samples by acquiring fluorescence images of NADH and Fp. The redox ratios, i.e. Fp/(Fp+NADH) and NADH/(Fp+NADH), provided a sensitive index of the mitochondrial redox state and were determined based on relative signal intensity ratios. Here we report the further development of the redox scanning technique by using a calibration method to quantify the nominal concentration of the fluorophores in tissues. The redox scanner exhibited very good linear response in the range of NADH concentration between 165-1318μM and Fp between 90-720 μM using snap-frozen solution standards. Tissue samples such as human tumor mouse xenografts and various mouse organs were redox-scanned together with adjacent NADH and Fp standards of known concentration at liquid nitrogen temperature. The nominal NADH and Fp concentrations as well as the redox ratios in the tissue samples were quantified by normalizing the tissue NADH and Fp fluorescence signal to that of the snap-frozen solution standards. This calibration procedure allows comparing redox images obtained at different time, independent of instrument settings. The quantitative multi-slice redox images revealed heterogeneity in mitochondrial redox state in the tissues.","PeriodicalId":16248,"journal":{"name":"Journal of Innovative Optical Health Sciences","volume":"2 4","pages":"375-385"},"PeriodicalIF":2.5,"publicationDate":"2009-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1142/S1793545809000681","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37449359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 26

MITOCHONDRIAL REDOX IMAGING FOR CANCER DIAGNOSTIC AND THERAPEUTIC STUDIES. 线粒体氧化还原成像用于癌症诊断和治疗研究。

IF 2.5 3区医学

Journal of Innovative Optical Health Sciences Pub Date : 2009-10-01 DOI: 10.1142/S1793545809000735

Lin Z Li, He N Xu, Mahsa Ranji, Shoko Nioka, Britton Chance

{"title":"MITOCHONDRIAL REDOX IMAGING FOR CANCER DIAGNOSTIC AND THERAPEUTIC STUDIES.","authors":"Lin Z Li, He N Xu, Mahsa Ranji, Shoko Nioka, Britton Chance","doi":"10.1142/S1793545809000735","DOIUrl":"10.1142/S1793545809000735","url":null,"abstract":"Mitochondrial redox states provide important information about energy-linked biological processes and signaling events in tissues for various disease phenotypes including cancer. The redox scanning method developed at the Chance laboratory about 30 years ago has allowed 3D high-resolution (~ 50 × 50 × 10 μm3) imaging of mitochondrial redox state in tissue on the basis of the fluorescence of NADH (reduced nicotinamide adenine dinucleotide) and Fp (oxidized flavoproteins including flavin adenine dinucleotide, i.e., FAD). In this review, we illustrate its basic principles, recent technical developments, and biomedical applications to cancer diagnostic and therapeutic studies in small animal models. Recently developed calibration procedures for the redox imaging using reference standards allow quantification of nominal NADH and Fp concentrations, and the concentration-based redox ratios, e.g., Fp/(Fp+NADH) and NADH/(Fp+NADH) in tissues. This calibration facilitates the comparison of redox imaging results acquired for different metabolic states at different times and/or with different instrumental settings. A redox imager using a CCD detector has been developed to acquire 3D images faster and with a higher in-plane resolution down to 10 μm. Ex vivo imaging and in vivo imaging of tissue mitochondrial redox status have been demonstrated with the CCD imager. Applications of tissue redox imaging in small animal cancer models include metabolic imaging of glioma and myc-induced mouse mammary tumors, predicting the metastatic potentials of human melanoma and breast cancer mouse xenografts, differentiating precancerous and normal tissues, and monitoring the tumor treatment response to photodynamic therapy. Possible future directions for the development of redox imaging are also discussed.","PeriodicalId":16248,"journal":{"name":"Journal of Innovative Optical Health Sciences","volume":"2 4","pages":"325-341"},"PeriodicalIF":2.5,"publicationDate":"2009-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4442014/pdf/nihms687594.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33338838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fc-DIRECTED ANTIBODY CONJUGATION OF MAGNETIC NANOPARTICLES FOR ENHANCED MOLECULAR TARGETING. 磁性纳米颗粒定向抗体偶联增强分子靶向。

IF 2.5 3区医学

Journal of Innovative Optical Health Sciences Pub Date : 2009-10-01 DOI: 10.1142/S1793545809000693

Robabeh Rezaeipoor, Renu John, Steven G Adie, Eric J Chaney, Marina Marjanovic, Amy L Oldenburg, Stephanie A Rinne, Stephen A Boppart

{"title":"Fc-DIRECTED ANTIBODY CONJUGATION OF MAGNETIC NANOPARTICLES FOR ENHANCED MOLECULAR TARGETING.","authors":"Robabeh Rezaeipoor, Renu John, Steven G Adie, Eric J Chaney, Marina Marjanovic, Amy L Oldenburg, Stephanie A Rinne, Stephen A Boppart","doi":"10.1142/S1793545809000693","DOIUrl":"https://doi.org/10.1142/S1793545809000693","url":null,"abstract":"In this study, we report the fabrication of engineered iron oxide magnetic nanoparticles (MNPs) functionalized with anti-human epidermal growth factor receptor type 2 (HER2) antibody to target the tumor antigen HER2. The Fc-directed conjugation of antibodies to the MNPs aids their efficient immunospecific targeting through free Fab portions. The directional specificity of conjugation was verified on a macrophage cell line. Immunofluorescence studies on macrophages treated with functionalized MNPs and free anti-HER2 antibody revealed that the antibody molecules bind to the MNPs predominantly through their Fc portion. Different cell lines with different HER2 expression levels were used to test the specificity of our functionalized nanoprobe for molecular targeting applications. The results of cell line targeting demonstrate that these engineered MNPs are able to differentiate between cell lines with different levels of HER2 expression.","PeriodicalId":16248,"journal":{"name":"Journal of Innovative Optical Health Sciences","volume":"2 4","pages":"387-396"},"PeriodicalIF":2.5,"publicationDate":"2009-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1142/S1793545809000693","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29915736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 19

PRELIMINARY STUDY ON SKIN CANCER DETECTION IN SENCAR MICE USING MUELLER OPTICAL COHERENCE TOMOGRAPHY. 穆勒光学相干断层扫描检测小鼠皮肤癌的初步研究。

IF 2.5 3区医学

Journal of Innovative Optical Health Sciences Pub Date : 2009-07-01 DOI: 10.1142/s1793545809000577

Miloš Todorović, Shuliang Jiao, George Stoica, Lihong V Wang

引用次数: 3

Live Imaging of Early Developmental Processes in Mammalian Embryos with Optical Coherence Tomography. 光学相干断层成像在哺乳动物胚胎早期发育过程中的实时成像。

IF 2.5 3区医学

Journal of Innovative Optical Health Sciences Pub Date : 2009-01-01 DOI: 10.1142/S1793545809000619

Kirill V Larin, Irina V Larina, Michael Liebling, Mary E Dickinson

引用次数: 53

ULTRASHORT PULSE MULTISPECTRAL NONLINEAR OPTICAL MICROSCOPY. 超短脉冲多光谱非线性光学显微镜。

IF 2.5 3区医学

Journal of Innovative Optical Health Sciences Pub Date : 2009-01-01 DOI: 10.1142/S1793545809000292

Adam M Larson, Anthony Lee, Po-Feng Lee, Kayla J Bayless, Alvin T Yeh

引用次数: 4

Simultaneous imaging of a lacZ-marked tumor and microvasculature morphology in vivo by dual-wavelength photoacoustic microscopy. 双波长光声显微镜同时成像lacz标记肿瘤和体内微血管形态。

IF 2.5 3区医学

Journal of Innovative Optical Health Sciences Pub Date : 2008-10-01 DOI: 10.1142/S1793545808000212

Li Li, Hao F Zhang, Roger J Zemp, Konstantin Maslov, Lihong Wang

引用次数: 46