Journal of Innovative Optical Health Sciences最新文献

Photobiomodulation mitigates LPS-induced astrocyte neuroinflammation via the STAT5A/SOCS3 axis 光生物调节通过STAT5A/SOCS3轴减轻脂多糖诱导的星形胶质细胞神经炎症

3区医学

Journal of Innovative Optical Health Sciences Pub Date : 2025-07-18 DOI: 10.1142/s1793545825500282

Wenjing Chen, Xiaotong Gu, Sheng‐Nan Wu, Feifan Zhou

{"title":"Photobiomodulation mitigates LPS-induced astrocyte neuroinflammation via the STAT5A/SOCS3 axis","authors":"Wenjing Chen, Xiaotong Gu, Sheng‐Nan Wu, Feifan Zhou","doi":"10.1142/s1793545825500282","DOIUrl":"https://doi.org/10.1142/s1793545825500282","url":null,"abstract":"Neuroinflammation plays an important role in the occurrence and development of neurological diseases. In addition to microglia, the role of astrocytes in neuroinflammation has gradually attracted attention. Photobiomodulation (PBM), as a non-invasive treatment, has been shown potential to alleviate inflammation of microglia or astrocytes. In this study, the spatiotemporal regulation and molecular mechanism of PBM on astrocytes were deeply explored by analyzing the effects and genomics at different time points. The results showed that PBM significantly attenuated the upregulation of inflammatory factors and mitochondrial dysfunction in astrocytes under LPS stimulation for 4[Formula: see text]h and 24[Formula: see text]h. RNA-seq analysis showed that the JAK-STAT pathway played an important role in the early stage of both LPS-induced astrocytic neuroinflammation and PBM-alleviated astrocytic neuroinflammation. Under PBM treatment, Stat5a translocation to the nucleus and upregulated Socs3 expression were observed in LPS-treated astrocytes, which may inhibit the overactivation of the JAK-STAT inflammatory signaling pathway and thus alleviate astrocyte inflammation. Taken together, this study provides new insight into the molecular mechanism of the potential application of PBM in the treatment of neuroinflammation.","PeriodicalId":16248,"journal":{"name":"Journal of Innovative Optical Health Sciences","volume":"19 01","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147906455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rabbit corneal damage threshold induced by a tunable laser system at 254 nm 254 nm可调谐激光系统诱导兔角膜损伤阈值

3区医学

Journal of Innovative Optical Health Sciences Pub Date : 2025-04-11 DOI: 10.1142/s1793545825500166

Luyao Zhu, Zaiping Nie, Ming Qiu, Ying Wang, Congling Zhou, Liejin Guo, Jiarui Wang, Zaifu Yang, Luguang Jiao

引用次数: 1

Deep learning-enhanced NIR-II fluorescence volumetric microscopy for dynamic 3D vascular imaging 深度学习增强的NIR-II荧光体积显微镜用于动态三维血管成像

3区医学

Journal of Innovative Optical Health Sciences Pub Date : 2025-03-28 DOI: 10.1142/s1793545825500130

Shiyi Peng, Yuhuang Zhang, Xuanjie Mou, Tianxiang Wu, Mingxi Zhang, Jun Qian

{"title":"Deep learning-enhanced NIR-II fluorescence volumetric microscopy for dynamic 3D vascular imaging","authors":"Shiyi Peng, Yuhuang Zhang, Xuanjie Mou, Tianxiang Wu, Mingxi Zhang, Jun Qian","doi":"10.1142/s1793545825500130","DOIUrl":"https://doi.org/10.1142/s1793545825500130","url":null,"abstract":"Three-dimensional (3D) visualization of dynamic biological processes in deep tissue remains challenging due to the trade-off between temporal resolution and imaging depth. Here, we present a novel near-infrared-II (NIR-II, 900–1880[Formula: see text]nm) fluorescence volumetric microscopic imaging method that combines an electrically tunable lens (ETL) with deep learning approaches for rapid 3D imaging. The technology achieves volumetric imaging at 4.2 frames per second (fps) across a 200[Formula: see text] [Formula: see text]m depth range in live mouse brain vasculature. Two specialized neural networks are utilized: a scale-recurrent network (SRN) for image enhancement and a cerebral vessel interpolation (CVI) network that enables 16-fold axial upsampling. The SRN, trained on two-photon fluorescence microscopic data, improves both lateral and axial resolution of NIR-II fluorescence wide-field microscopic images. The CVI network, adapted from video interpolation techniques, generates intermediate frames between acquired axial planes, resulting in smooth and continuous 3D vessel reconstructions. Using this integrated system, we visualize and quantify blood flow dynamics in individual vessels and are capable of measuring blood velocity at different depths. This approach maintains high lateral resolution while achieving rapid volumetric imaging, and is particularly suitable for studying dynamic vascular processes in deep tissue. Our method demonstrates the potential of combining optical engineering with artificial intelligence to advance biological imaging capabilities.","PeriodicalId":16248,"journal":{"name":"Journal of Innovative Optical Health Sciences","volume":"18 03","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147915983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Near-infrared Aza-BODIPY nanoparticles based on D-A-D structure for bioimaging 基于D-A-D结构的近红外Aza-BODIPY纳米颗粒生物成像

3区医学

Journal of Innovative Optical Health Sciences Pub Date : 2025-01-24 DOI: 10.1142/s1793545825410032

Haitao Liao, Qingxuan Meng, Yuhao Li, Senqiang Zhu, Rui Liu, Hongjun Zhu

{"title":"Near-infrared Aza-BODIPY nanoparticles based on D-A-D structure for bioimaging","authors":"Haitao Liao, Qingxuan Meng, Yuhao Li, Senqiang Zhu, Rui Liu, Hongjun Zhu","doi":"10.1142/s1793545825410032","DOIUrl":"https://doi.org/10.1142/s1793545825410032","url":null,"abstract":"Optical imaging in vivo holds significant implications for disease diagnosis, and nanoprobes with near-infrared (NIR) emission leverage the deep tissue penetration and high spatiotemporal resolution provided by NIR light, demonstrating considerable application potential. This study presents the design and synthesis of three nitrogen-doped boron–dipyrrin (Aza-BODIPY) molecules: Aza–BDP–OCH 3 , Aza–BDP–OH, and Aza–BDP-I. Leveraging the strong electron-accepting properties of the Aza-BODIPY core, we developed a donor–acceptor–donor (D-A-D) structure for Aza–BDP–OCH 3 through modifications with triphenylamine and methoxy groups, resulting in NIR fluorescence. Aza–BDP–OH was obtained via demethylation using boron tribromide, whereas Aza–BDP-I was synthesized by introducing iodine into Aza–BDP–OCH 3 . These three molecules self-assemble with the amphiphilic polymer PMHC[Formula: see text]-mPEG to form nanoparticles (NPs), yielding optical nanoprobes. The resulting NPs exhibit NIR emission, good water solubility, and biocompatibility. At a concentration of 100[Formula: see text] [Formula: see text], these NPs demonstrate low biological toxicity, highlighting their potential for biological applications. Following tail vein injection, Aza–BDP-I NPs accumulate in tumors and effectively illuminate them via the enhanced permeability and retention (EPR) effect. Furthermore, these organic NPs were metabolized by the liver. Therefore, Aza-BODIPY-based NIR fluorescent NPs offer a promising platform for the development of in vivo optical nanoprobes.","PeriodicalId":16248,"journal":{"name":"Journal of Innovative Optical Health Sciences","volume":"18 03","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147909265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Correlating NAD(P)H lifetime shifts to tamoxifen resistance in breast cancer cells: A metabolic screening study with time-resolved flow cytometry. 乳腺癌细胞中NAD(P)H寿命变化与他莫昔芬耐药性的相关性：一项使用时间分辨流式细胞术的代谢筛选研究

IF 2.3 3区医学

Journal of Innovative Optical Health Sciences Pub Date : 2025-01-01 DOI: 10.1142/s1793545824500202

Samantha Valentino, Karla Ortega-Sandoval, Kevin D Houston, Jessica P Houston

{"title":"Correlating NAD(P)H lifetime shifts to tamoxifen resistance in breast cancer cells: A metabolic screening study with time-resolved flow cytometry.","authors":"Samantha Valentino, Karla Ortega-Sandoval, Kevin D Houston, Jessica P Houston","doi":"10.1142/s1793545824500202","DOIUrl":"10.1142/s1793545824500202","url":null,"abstract":"Time-resolved flow cytometry (TRFC) was used to measure metabolic differences in estrogen receptor-positive breast cancer cells. This specialty cytometry technique measures fluorescence lifetimes as a single-cell parameter thereby providing a unique approach for high-throughput cell counting and screening. Differences in fluorescence lifetime were detected and this was associated with sensitivity to the commonly prescribed therapeutic tamoxifen. Differences in fluorescence lifetime are attributed to the binding states of the autofluorescent metabolite NAD(P)H. The function of NAD(P)H is well described and in general involves cycling from a reduced to oxidized state to facilitate electron transport for the conversion of pyruvate to lactate. NAD(P)H fluorescence lifetimes depend on the bound or unbound state of the metabolite, which also relates to metabolic transitions between oxidative phosphorylation and glycolysis. To determine if fundamental metabolic profiles differ for cells that are sensitive to tamoxifen compared to those that are resistant, large populations of MCF-7 breast cancer cells were screened and fluorescence lifetimes were quantified. Additionally, metabolic differences associated with tamoxifen sensitivity were measured with a Seahorse HS mini metabolic analyzer (Agilent Technologies Inc. Santa Clara, CA) and confocal imaging. Results show that tamoxifen-resistant breast cancer cells have increased utilization of glycolysis for energy production compared to tamoxifen-sensitive breast cancer cells. This work is impacting because it establishes an early step toward developing a reliable screening technology in which large cell censuses can be differentiated for drug sensitivity in a label-free fashion.","PeriodicalId":16248,"journal":{"name":"Journal of Innovative Optical Health Sciences","volume":"18 1","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11841857/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143468321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recombinant PASylated nanobody probes with improved blood circulation and tumor targeting 具有改善血液循环和肿瘤靶向性的重组PASylated纳米体探针

3区医学

Journal of Innovative Optical Health Sciences Pub Date : 2024-11-08 DOI: 10.1142/s1793545825410019

Yicheng Yang, Lingyue Jin, You Zhang, Siyu Zhou, Weijun Wei, Gang Huang, Changfeng Wu

引用次数: 5

Machine learning-enhanced SERS for accurate azoospermia diagnosis via seminal plasma exosome analysis 机器学习增强SERS通过精浆外泌体分析准确诊断无精子症

3区医学

Journal of Innovative Optical Health Sciences Pub Date : 2024-10-30 DOI: 10.1142/s1793545825500038

Jiarui Wang, Shiyan Jiang, Jiaxin Shi, Jing Wang, Shengrong Du, Zufang Huang

引用次数: 3

Clickable HaloTag ligands for live cell labeling and imaging 可点击的HaloTag配体用于活细胞标记和成像

3区医学

Journal of Innovative Optical Health Sciences Pub Date : 2024-10-04 DOI: 10.1142/s1793545824410037

Yunhe Luo, Siyu Zhou, Mingyue Gong, Changfeng Wu, Xiaofeng Fang

{"title":"Clickable HaloTag ligands for live cell labeling and imaging","authors":"Yunhe Luo, Siyu Zhou, Mingyue Gong, Changfeng Wu, Xiaofeng Fang","doi":"10.1142/s1793545824410037","DOIUrl":"https://doi.org/10.1142/s1793545824410037","url":null,"abstract":"Self-labeling protein (SLP) tags, such as HaloTag, have gained considerable interest as advanced tools for live cell labeling. However, the chloroalkane-based substrates that can be directly used for protein labeling are limited. Here, we report two bioorthogonal small molecule linkers, chloroalkane-tetrazine (CA-Tz) and chloroalkane-azide (CA-N 3 ), which can penetrate cell membranes and facilitate click chemistry-based labeling in live cells. We compare their labeling capability using two clickable silicon rhodamine dyes (SiR-PEG 3 -TCO and SiR-PEG 4 -DBCO). Confocal imaging results demonstrate that using CA-Tz and SiR-PEG 3 -TCO dye exhibits superior intracellular labeling with low nonspecific signals. We subsequently compared the photostability of SiR dyes with that of green fluorescent proteins (mEmerald). Total internal reflection fluorescence (TIRF) imaging indicates that SiR dyes exhibit superior photostability under identical excitation conditions, making them suitable for long-term cell imaging. Furthermore, SiR dyes labeling also shows high structure retention for the fourth-order super-resolution optical fluctuation imaging (SOFI) compared to fluorescent proteins. This study presents clickable HaloTag linkers as effective tools for live cell labeling and imaging, highlighting the high-quality labeling of chloroalkane linkers and clickable dyes for live cell imaging.","PeriodicalId":16248,"journal":{"name":"Journal of Innovative Optical Health Sciences","volume":"18 03","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147916191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

A photo-triggered bioactive nanohydrogel loaded with niobium carbide for stem cell osteogenic differentiation 负载碳化铌的光触发生物活性纳米水凝胶用于干细胞成骨分化

3区医学

Journal of Innovative Optical Health Sciences Pub Date : 2024-06-24 DOI: 10.1142/s1793545824420021

Ya Li, Yuanzhi Zhang, Derong Xu, Ming Gao, Kunpeng Su, Yingjie Chen, Chen Wang, Xinming Geng, Youchang Liu, Chuanli Zhou, Meng Qiu

{"title":"A photo-triggered bioactive nanohydrogel loaded with niobium carbide for stem cell osteogenic differentiation","authors":"Ya Li, Yuanzhi Zhang, Derong Xu, Ming Gao, Kunpeng Su, Yingjie Chen, Chen Wang, Xinming Geng, Youchang Liu, Chuanli Zhou, Meng Qiu","doi":"10.1142/s1793545824420021","DOIUrl":"https://doi.org/10.1142/s1793545824420021","url":null,"abstract":"Photo-Cross-Linkable hydrogel has attracted immense interest in the regeneration of bone repair and regeneration strategies due to its superior biocompatibility and tunable mechanical properties. Recently, Nb was reported to strongly promote the bone regeneration process via an accelerated osteoblast-modulated alkaline phosphatase activity mechanism. In particular, Nb 2 C MXenes have drawn widespread attention due to their excellent biocompatibility and ability to induce bone formation. However, the easy agglomeration of Nb 2 C nanosheets and subsequent low cell endocytosis efficacy greatly suppressed the osteogenesis effect. In this study, a subtractive nanopore-engineered Nb 2 C MXene was prepared through a microwave combustion method, gelatin methacrylate was used as the carrier hydrogel, and the photo-triggered Porous-Nb 2 C@GelMA hydrogel was fabricated by a photo-triggered process. The pore-forming strategy not only successfully improved the distribution of Nb 2 C and formed more homogenous Porous-Nb 2 C@GelMA hydrogels but also guided bone marrow mesenchymal stem cells (BMSCs) toward osteoblast differentiation. Porous Nb 2 C provided convenient cellular grasping and endocytosis for BMSCs, which further created a favorable environment for differentiation and osteogenesis. This, in turn, leads to an increase in the expression of osteogenic markers, such as ALP and ARS, as well as osteogenic factors, such as BMP-2, COL-1, OCN, and OPN. Consequently, enhancing the regenerative microenvironment by incorporating porous Nb 2 C composite hydrogels shows promise for application in bone regeneration.","PeriodicalId":16248,"journal":{"name":"Journal of Innovative Optical Health Sciences","volume":"18 04","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147332564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Label-free in-vivo classification and tracking of red blood cells and platelets using Dynamic-YOLOv4 network 使用动态-YOLOv4 网络对红细胞和血小板进行无标记体内分类和跟踪

IF 2.5 3区医学

Journal of Innovative Optical Health Sciences Pub Date : 2024-05-25 DOI: 10.1142/s1793545824500093

Caizhong Guan, Bin He, Hongting Zhang, Shangpan Yang, Yang Xu, Honglian Xiong, Yaguang Zeng, Mingyi Wang, Xunbin Wei

{"title":"Label-free in-vivo classification and tracking of red blood cells and platelets using Dynamic-YOLOv4 network","authors":"Caizhong Guan, Bin He, Hongting Zhang, Shangpan Yang, Yang Xu, Honglian Xiong, Yaguang Zeng, Mingyi Wang, Xunbin Wei","doi":"10.1142/s1793545824500093","DOIUrl":"https://doi.org/10.1142/s1793545824500093","url":null,"abstract":"In-vivo flow cytometry is a noninvasive real-time diagnostic technique that facilitates continuous monitoring of cells without perturbing their natural biological environment, which renders it a valuable tool for both scientific research and clinical applications. However, the conventional approach for improving classification accuracy often involves labeling cells with fluorescence, which can lead to potential phototoxicity. This study proposes a label-free in-vivo flow cytometry technique, called dynamic YOLOv4 (D-YOLOv4), which improves classification accuracy by integrating absorption intensity fluctuation modulation (AIFM) into YOLOv4 to demodulate the temporal features of moving red blood cells (RBCs) and platelets. Using zebrafish as an experimental model, the D-YOLOv4 method achieved average precisions (APs) of 0.90 for RBCs and 0.64 for thrombocytes (similar to platelets in mammals), resulting in an overall AP of 0.77. These scores notably surpass those attained by alternative network models, thereby demonstrating that the combination of physical models with neural networks provides an innovative approach toward developing label-free in-vivo flow cytometry, which holds promise for diverse in-vivo cell classification applications.","PeriodicalId":16248,"journal":{"name":"Journal of Innovative Optical Health Sciences","volume":"40 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141188217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0