Fc-DIRECTED ANTIBODY CONJUGATION OF MAGNETIC NANOPARTICLES FOR ENHANCED MOLECULAR TARGETING.

IF 2.3 3区医学 Q2 OPTICS

Journal of Innovative Optical Health Sciences Pub Date : 2009-10-01 DOI:10.1142/S1793545809000693

Robabeh Rezaeipoor, Renu John, Steven G Adie, Eric J Chaney, Marina Marjanovic, Amy L Oldenburg, Stephanie A Rinne, Stephen A Boppart

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引用次数: 19

Abstract

In this study, we report the fabrication of engineered iron oxide magnetic nanoparticles (MNPs) functionalized with anti-human epidermal growth factor receptor type 2 (HER2) antibody to target the tumor antigen HER2. The Fc-directed conjugation of antibodies to the MNPs aids their efficient immunospecific targeting through free Fab portions. The directional specificity of conjugation was verified on a macrophage cell line. Immunofluorescence studies on macrophages treated with functionalized MNPs and free anti-HER2 antibody revealed that the antibody molecules bind to the MNPs predominantly through their Fc portion. Different cell lines with different HER2 expression levels were used to test the specificity of our functionalized nanoprobe for molecular targeting applications. The results of cell line targeting demonstrate that these engineered MNPs are able to differentiate between cell lines with different levels of HER2 expression.

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磁性纳米颗粒定向抗体偶联增强分子靶向。

在这项研究中，我们报道了用抗人表皮生长因子受体2型(HER2)抗体功能化的工程氧化铁磁性纳米颗粒(MNPs)的制备，以靶向肿瘤抗原HER2。fc导向的抗体与MNPs的结合有助于它们通过游离Fab部分进行有效的免疫特异性靶向。在巨噬细胞系上验证了偶联的定向特异性。用功能化MNPs和游离抗her2抗体处理巨噬细胞的免疫荧光研究显示，抗体分子主要通过MNPs的Fc部分与MNPs结合。我们利用不同HER2表达水平的细胞系来测试我们的功能化纳米探针在分子靶向应用中的特异性。细胞系靶向的结果表明，这些工程化的MNPs能够区分具有不同水平HER2表达的细胞系。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Innovative Optical Health Sciences OPTICS-RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING

CiteScore

4.50

自引率

20.00%

发文量

审稿时长

>12 weeks

期刊介绍： JIOHS serves as an international forum for the publication of the latest developments in all areas of photonics in biology and medicine. JIOHS will consider for publication original papers in all disciplines of photonics in biology and medicine, including but not limited to: -Photonic therapeutics and diagnostics- Optical clinical technologies and systems- Tissue optics- Laser-tissue interaction and tissue engineering- Biomedical spectroscopy- Advanced microscopy and imaging- Nanobiophotonics and optical molecular imaging- Multimodal and hybrid biomedical imaging- Micro/nanofabrication- Medical microsystems- Optical coherence tomography- Photodynamic therapy. JIOHS provides a vehicle to help professionals, graduates, engineers, academics and researchers working in the field of intelligent photonics in biology and medicine to disseminate information on the state-of-the-art technique.