Journal of Industrial Microbiology & Biotechnology最新文献

{"title":"Metagenomic mining unveils a novel GH130 enzyme with exclusive xylanase activity over a wide temperature and pH ranges.","authors":"Amr A Hemeda, Sara A Zahran, Marwa Ali-Tammam, Menna A Ewida, Mona T Kashef, Aymen S Yassin, Avishek Mitra, Noha H Youssef, Mostafa S Elshahed","doi":"10.1093/jimb/kuaf006","DOIUrl":"10.1093/jimb/kuaf006","url":null,"abstract":"<p><p>The equine gut harbors a diverse microbial community and represents a rich source of carbohydrate-active enzymes (CAZymes). To identify and characterize potentially novel CAZymes from a horse's hindgut metagenome, shotgun metagenomic sequencing was performed on DNA extracted from a stool sample of a male horse, followed by CAZyme annotation. Here, we report on the characterization of a novel enzyme (AH2) that was identified, synthesized, cloned, and characterized from the obtained CAZyme dataset. AH2 was identified as a GH130 family member and displayed exclusive xylanase activity, a trait hitherto unreported in prior characterization of GH130 CAZymes. AH2 displayed an optimal activity at a pH of 5.6 and a temperature of 50°C. AH2 maintained significant activity across a pH range of 4-10 (62-72%) and temperatures of 30-70°C (77-86%). The enzyme had remarkable stability, with minimal reductions in activity across a temperature range of 4-70°C and pH levels of 3, 7, and 9. Docking studies identified AH2's amino acids (Glu90 and Glu149) to be involved in substrate binding. Molecular dynamics simulation confirmed the structural stability of AH2 at pH 5.6 and 50°C, further supporting its resilience under these conditions. Our results expand on the known activities associated with the GH130 CAZyme family and demonstrate that the horse gut metagenome represents an unexplored source of novel CAZymes.</p><p><strong>One-sentence summary: </strong>A novel activity for members of the CAZyme family GH130.</p>","PeriodicalId":16092,"journal":{"name":"Journal of Industrial Microbiology & Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11905756/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143557068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbial biosynthesis of rare cannabinoids.","authors":"Chunsheng Yan, Ikechukwu C Okorafor, Colin W Johnson, Kendall N Houk, Neil K Garg, Yi Tang","doi":"10.1093/jimb/kuaf013","DOIUrl":"10.1093/jimb/kuaf013","url":null,"abstract":"<p><p>∆9-tetrahydrocannabinol (∆9-THC) and cannabidiol are the most abundant natural cannabinoids isolated from the different cultivars of the Cannabis plant. Other natural ∆9-THC analogs, especially those with different alkyl chain substitutions, display different and potent bioactivity. However, these rare cannabinoids are typically isolated in minuscule amounts and are difficult to synthesize. Targeted microbial biosynthesis can therefore be an attractive route to access such molecules. Here, we report the development of a Saccharomyces cerevisiae host to biosynthesize 2 rare cannabinoids from simple sugars. The yeast host is engineered to accumulate excess geranyl pyrophosphate, to overexpress a fungal pathway to 2,4-dihydroxy-6-alkyl-benzoic acids, as well as the downstream UbiA-prenyltransferase and ∆9-tetrahydrocannabinolic acid synthase. Two rare cannabinoid acids, the C1-substituted ∆9-tetrahydrocannabiorcolic acid (∼16 mg/L) and the C7-substituted ∆9-tetrahydrocannabiphorolic acid (∼5 mg/L) were obtained from this host; the latter was thermally decarboxylated to give ∆9-tetrahydrocannabiphorol. Given the diversity of fungal biosynthetic gene clusters that can produce resorcylic acids, this microbial platform offers the potential to produce other rare and new-to-nature cannabinoids. One Sentence Summary:  Saccharomyces cerevisiae as a host to produce rare cannabinoids.</p>","PeriodicalId":16092,"journal":{"name":"Journal of Industrial Microbiology & Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12134893/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143996141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacteria-powered self-healing concrete: Breakthroughs, challenges, and future prospects.","authors":"Ibrahim M Elgendy, Nehal E Elkaliny, Hoda M Saleh, Gehad O Darwish, Mervt M Almostafa, Kamel Metwally, Galal Yahya, Yehia A-G Mahmoud","doi":"10.1093/jimb/kuae051","DOIUrl":"10.1093/jimb/kuae051","url":null,"abstract":"<p><p>In a world where concrete structures face constant degradation from environmental forces, a revolutionary solution has emerged: bio-self-healing concrete. This innovation involves embedding dormant bacteria within the concrete mix, poised to spring into action when cracks form. As moisture seeps into the cracks, these bacterial agents are activated, consuming nutrients and converting them into calcium carbonate, a natural substance that fills and repairs the fractures, restoring the material's integrity. This fascinating process represents a cutting-edge approach to maintaining concrete infrastructure, turning once-vulnerable materials into self-sustaining systems capable of healing themselves. The ongoing research into bio-self-healing concrete is focused on selecting bacterial strains that can withstand the extreme conditions within concrete, including its highly alkaline environment. The bacteria must also form resilient spores, remaining viable until they are needed for repair. Additionally, the study explores various challenges associated with this technology, such as the cost of production, the bacteria's long-term viability, and their potential environmental impact. Advancements in genetic engineering and smart technology are being explored to enhance these bacterial strains, making them more efficient and robust in their role as microscopic repair agents. This review delves into the potential of bio-self-healing concrete to revolutionize how we approach infrastructure maintenance, offering a glimpse into a future where concrete structures not only endure but actively repair themselves, extending their lifespan and reducing the need for costly repairs.</p><p><strong>One-sentence summary: </strong>Bio-self-healing concrete utilizes bacteria that activate upon crack formation to repair structures by producing calcium carbonate, offering a sustainable solution to prolong the lifespan of concrete infrastructure.</p>","PeriodicalId":16092,"journal":{"name":"Journal of Industrial Microbiology & Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11730074/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142824235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pseudoalteromonas agarivorans-derived novel ulvan lyase of polysaccharide lyase family 40: Potential application of ulvan and partially hydrolyzed products in cosmetic industry.","authors":"Navindu Dinara Gajanayaka, Eunyoung Jo, Minthari Sakethanika Bandara, Svini Dileepa Marasinghe, Sachithra Amarin Hettiarachchi, Sithumini Wijewickrama, Gun-Hoo Park, Chulhong Oh, Youngdeuk Lee","doi":"10.1093/jimb/kuaf004","DOIUrl":"10.1093/jimb/kuaf004","url":null,"abstract":"<p><p>Ulvan is a complex sulfated polysaccharide in the cell walls of green algae with extensive applications in food, pharmaceutical, and agricultural industries, prompting extensive studies on ulvan, its oligosaccharides, monosaccharides, and cost-effective depolymerization methods. Our primary objectives were to investigate novel ulvan-utilizing marine bacteria, perform recombinant engineering of genes responsible for ulvan depolymerization, and determine their potential industrial applications. Samples were collected from Jeju Island, which is a South Korean region with significant excessive green algal growth, especially that of Ulva species. The marine bacterium Pseudoalteromonas agarivorans efficiently uses ulvan as its primary carbon source, indicating its potential for ulvan degradation. Through whole-genome sequencing the paul40 gene, which is a polysaccharide lyase family 40 (PL40) member, was identified and subsequently engineered into the pET-16b vector for expression as a His-tagged 95 kDa fusion protein. The ulvan depolymerization process was evaluated and confirmed using various analytical techniques including dinitrosalicylic acid assay, thin-layer chromatography, and gel permeation chromatography. Optimal enzyme activity occurred at 35°C, pH 8.0 in phosphate buffer, and 2.5 mM of NaCl. Furthermore, enzyme characterization and specific activity measurements were performed. This study is the first to report hyaluronidase and elastase inhibition by ulvan and its derivatives along with the characterization of an ulvan lyase enzyme from the PL40 family.</p><p><strong>One-sentence summary: </strong>This study reports the identification and recombinant expression of a novel ulvan-degrading enzyme from Pseudoalteromonas agarivorans, demonstrating its potential for cosmetic industrial applications by revealing ulvan's and partially hydrolyzed ulvan's hyaluronidase and elastase inhibition properties.</p>","PeriodicalId":16092,"journal":{"name":"Journal of Industrial Microbiology & Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11835017/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143370648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel supplementation of Fe3O4-doped green carbonized nanoparticles on hydrogenases genes and microbial biodiversity for enhancing biohydrogen yield in dark fermentation microbial electrohydrogenesis cells.","authors":"Hikmatullah Ahmadi, Anam Jalil, Sohail Khan, Irfan Ali Phulpoto, Zhang Chengyu, Zhisheng Yu","doi":"10.1093/jimb/kuaf016","DOIUrl":"10.1093/jimb/kuaf016","url":null,"abstract":"<p><p>Achieving high-purity biohydrogen (Bio-H₂) production necessitates the suppression of hydrogenotrophic methanogens, as their activity can impede hydrogen yield. Various inoculum pretreatments have been employed to suppress methane-producing microorganisms; however, these methods can negatively impact the enzymatic activity of hydrogen-producing microorganisms, thereby reducing hydrogen production. To address this challenge, this research investigates a novel approach to enhance Bio-H₂ production by activating microbial enzymes using magnetite Fe₃O4-doped carbonized nanoparticles (NPs) derived from vegetable leaves (VLCFe₃O4-NPs) within a coupled dark fermentation-microbial Electrohydrogenesis system. Characterization results revealed that VLCFe₃O4-NPs exhibited cubic and spherical morphologies, with a small diameter of 1 ± 100 nm and a mean crystallite size of 38.1 nm, indicating high purity. Fermentation tests investigated the impact of different nanoparticle dosages on Bio-H₂ generation, hydrogenase gene expression (Fe-Fe and Ni-Fe), and microbial biodiversity. Bio-H₂ production significantly improved with 500 mg/L VLCFe₃O4-NPs, yielding 1.2-fold more than the control group, while even a low dose of 25 mg/L resulted in a 0.22-fold increase. Relative gene expression analysis using qPCR and the 2-ΔΔCT method demonstrated a 30-fold increase in Cbei 1773 (Fe-Fe hydrogenase) and a 23-fold increase in hucL (Ni-Fe hydrogenase) gene expression, along with an increase in 16S rDNA. Additionally, the abundance of biohydrogen-producing bacteria, Clostridium_sensu_stricto_1 and Clostridium_sensu_stricto_11, increased by 14.3% and 11.1%, respectively, compared to 4.9% and 3.9% in the control group. This research indicates that VLCFe₃O4-NPs offer an eco-friendly solution for boosting biohydrogen production within microbial electrohydrogenesis cells with dark fermentation systems, thereby supporting sustainable bioenergy generation. One-sentence summary: Green carbonized nanoparticles Fe3O4-doped have been shown to turn on the genes of bacteria (Fe-Fe and Ne-Fe) and increase the biodiversity of microbes, both of which are important for biohydrogen production.</p>","PeriodicalId":16092,"journal":{"name":"Journal of Industrial Microbiology & Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12259281/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144511990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biocide-resistant Pseudomonas oleovorans isolated from water-based coatings used in construction.","authors":"Muatasem Latif Ali, Lionel Ferrieres, Tuulia Hyötyläinen, Jana Jass","doi":"10.1093/jimb/kuaf015","DOIUrl":"10.1093/jimb/kuaf015","url":null,"abstract":"<p><p>Biocides are crucial in industrial applications to minimize microbial growth and prevent product spoilage. Water-based construction coatings are susceptible to microbial contamination during manufacturing and storage and this adversely impacts product properties, reduces shelf-life, and leads to substantial commercial losses. The future trend to lower the biocide concentrations in water-based coatings raises concerns about the emergence of biocide-resistant microbes. This study aims to identify and characterize the biocide-resistant microbe isolated from construction water-based coating materials to better understand its mechanisms of resistance. A total of 63 samples were collected from spoiled products, raw materials, and water from a manufacturing facility, and Pseudomonas oleovorans P4A were identified in all biocides-treated samples. A comparison between a P. oleovorans reference strain, 1045, and the P4A isolate revealed distinct colony morphology, growth rate and sensitivity to biocides and antibiotics. The P4A isolate was threefold more resistant to 5-chloro-2-methyl-isothiazolin-3-one and 1.5-fold more resistant to benzothiazolinone (BIT) compared to the reference strain. Conversely, it was 1.4-fold more sensitive to methylisothiazolinone (MIT) compared to the reference strain. No cross-resistance to antibiotics was observed. Metabolomic analysis using liquid chromatography combined with high-resolution mass spectrometry of lipids and polar metabolites showed that P4A had a relatively higher amount of lipids, while 1045 had a relatively higher amount of polar metabolites identified. A significant difference in lipid composition, specifically in diacylglycerol, phosphatidic acid, phosphatidylcholine, and phosphatidylserine was observed between P. oleovorans strains 1045 and P4A. These distinctions highlight increased lipid metabolism in P. oleovorans P4A and this may contribute to its adaptation to biocides. Microbial resistance can directly affect the effectiveness of these products, leading to an increased need for frequent maintenance and replacement, safety concerns, and environmental implications. One-Sentence Summary: Biocide-resistant Pseudomonas oleovorans isolate exhibited reduced growth rate and increased lipid levels relative to the reference strain.</p>","PeriodicalId":16092,"journal":{"name":"Journal of Industrial Microbiology & Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12231568/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144505959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploration of alternative microfiltration modalities for the harvest and clarification of diverse recombinant proteins from high-density E. coli culture and lysate using hollow fibre, flat sheet cassette, and vibro membrane filtration technologies.","authors":"Jennifer Reid, Joyce Ni, Airong Chen, Patricia Gomes, Andrew Szto, Analyn Yu, Angela Luo, Belinda Kong, Calvin Adams, Neveathan Jeyachandran, Anumta Amir, Xavier Teixeira, Tao Yuan, Cédric Charretier","doi":"10.1093/jimb/kuaf008","DOIUrl":"10.1093/jimb/kuaf008","url":null,"abstract":"<p><p>Industrial bioprocess optimization has significantly increased the productivity of biomass and biologics in upstream production. Such process improvement in fermentation often translates to challenges in recovering intracellularly expressed recombinant proteins due to increased matrix complexity, resulting in a higher performance burden in midstream. Tangential flow filtration (TFF) is a popular industry standard for buffer exchange and protein separation from cellular debris. However, due to variations in the physicochemical properties of recombinant proteins, solutions for E. coli-based protein clarification remain challenging and often necessitate extensive exploration and process optimization. With growing options in filtration-based technologies, the identification of a near-universal clarification platform is desirable to accelerate bioprocess development overall. In this study, three TFF modalities, hollow fibre (HF), flat-sheet cassette (CAS), and vibro membrane filtration (VMF), were assessed in parallel to evaluate their clarification performance for three E. coli recombinant proteins with different biochemical properties. Reverse phase liquid chromatography data showed target protein recovery was uniformly higher for VMF than HF at equivalent loading. Cell density and lysate protein load were comparable for HF and VMF, and lower for CAS. These results support the choice of VMF and HF as easily optimized and operated TFF modalities for clarification of recombinant protein from complex crude bacterial matrix, where either can be efficiently performed with ease and minimum supervision. Both TFF applications were successfully demonstrated in primary cell harvest, cell wash and cell lysate clarification, for E. coli-based recombinant proteins.</p><p><strong>One-sentence summary: </strong>High-density E. coli microfiltration and lysate clarification were tested for three diverse recombinant proteins, where hollow fibre and vibro membrane filtration outperformed flat sheet cassette in terms of process time, suspended solid loading, and target protein recovery.</p>","PeriodicalId":16092,"journal":{"name":"Journal of Industrial Microbiology & Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12022607/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143788516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular evolution of nucleoside deoxyribosyl transferase to enhance the activity toward 2'-fluoro-2'-deoxynucleoside.","authors":"Su-Been Yang, Yeon-Jin Yoo, Kanghyun Choi, Byungkyun Kim, Si-Sun Choi, Seung-Hoon Kang, Eung-Soo Kim","doi":"10.1093/jimb/kuaf005","DOIUrl":"10.1093/jimb/kuaf005","url":null,"abstract":"<p><p>Nucleoside deoxyribosyl transferase (NDT) is an enzyme that catalyzes the transfer of purine and pyrimidine bases between 2'-deoxyribonucleosides and is widely used for synthesizing nucleoside analogs in various biotechnological applications. While NDT exhibits high activity toward natural nucleosides, its activity toward unnatural nucleoside analogs is significantly lower. Previously, the NDT mutant named fNDT(L59Q) was identified displaying 4.4-fold higher activity toward 2'-fluoro-2'-deoxyuridine (2FDU). In this study, molecular evolution strategies using error-prone PCR were employed to further generate mutant enzymes with enhanced activity toward 2FDU. After two rounds of mutational screening, two mutant clones that exhibited high activity against 2FDU were identified as fNDT-i1 (V52A) and fNDT-i2 (L28I), respectively. A double mutant, fNDT-i4, was subsequently constructed by combining the V52A and L28I mutations. Whole-cell-based activity measurements showed that fNDT-i4 exhibited 4.0- and 20.6-fold higher activity at 40°C and 50°C, respectively, compared to the wild-type NDT. The detailed characterization of the purified enzymes conducted under various conditions, including temperature, pH, thermal stability, and enzyme kinetics experiments, showed that fNDT-i1 and fNDT-i4 exhibited 3.1- and 3.7-fold higher catalytic efficiency, respectively than wild-type NDT. The L59Q mutation was identified as a key factor in improving the thermal stability, whereas the V52A and L28I mutations were critical for improving substrate affinity and reaction efficiency. These findings provide the potential of fNDT-i1 and fNDT-i4 as highly efficient biocatalysts for developing industrially relevant nucleoside analog synthesis.</p><p><strong>One-sentence summary: </strong>The nucleoside deoxyribosyl transferase mutant were engineered to enhance biological activity and physical resistance for production of fluorinated deoxynucleoside as a raw material of oligonucleotide therapeutics.</p>","PeriodicalId":16092,"journal":{"name":"Journal of Industrial Microbiology & Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11892431/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143501903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adoption of a novel medium for the industrial (3000 L) production of Serendipita indica employing a nutrient limitation strategy using insoluble carbon and phosphate sources.","authors":"Jubair Al Rashid, Md Abuhena, Md Dilshad Karim, Lutfur Rahman, Jingjing Wang, Zhiyong Huang","doi":"10.1093/jimb/kuaf009","DOIUrl":"https://doi.org/10.1093/jimb/kuaf009","url":null,"abstract":"<p><p>The use of the endophytic fungus Serendipita indica has rapidly increased due to its wide range of host species, ability to foster plant-growth, and ability to confer tolerance to a number of stresses. However, its industrial-scale production is still in its infancy due to its low-biomass yield and prolonged cultivation time. Thus far, Hill-Kafer medium has traditionally been used for S. indica cultivation, resulting in lower yields and excessively long incubation times. Here, we adopted a simple insoluble carbon and phosphate input medium for rapidly generating high biomass. We developed and optimized the SIF1 medium, achieving maximum biomass production (424.5 ± 1.9 g/L), significantly outperforming Hill-Kafer medium. Statistical optimization of SIF1 identified optimal levels (15 g/L oats, 7.5 g/L tricalcium phosphate, 95-hr incubation). Validated results in the laboratory (FUS-10 L: 484.4 ± 4.7), pilot (300 L: 496.5 ± 7 g/L), and industrial (3000L: 492.4 ± 7.1 g/L) bioreactors proved the efficacy of SIF1. Compared to Hill-Kafer (54.8 ± 3.7 g/L), SIF1 showed nine-fold higher biomass productivity and reduced cultivation time by approximately 6 days. Based on our findings, it appears that SF1 will be a highly efficient medium for producing S. indica on an industrial scale and expanding its use.</p><p><strong>One-sentence summary: </strong>This study presents a rapid industrial production strategy for the beneficial fungus Serendipita indica, providing a scalable solution for wider applications and contributing to global food security and environmental sustainability.</p>","PeriodicalId":16092,"journal":{"name":"Journal of Industrial Microbiology & Biotechnology","volume":"52 ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12010874/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143969704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Purple non-sulfur bacteria for biotechnological applications.","authors":"Hailee M Morrison, Arpita Bose","doi":"10.1093/jimb/kuae052","DOIUrl":"10.1093/jimb/kuae052","url":null,"abstract":"<p><p>In this review, we focus on how purple non-sulfur bacteria can be leveraged for sustainable bioproduction to support the circular economy. We discuss the state of the field with respect to the use of purple bacteria for energy production, their role in wastewater treatment, as a fertilizer, and as a chassis for bioplastic production. We explore their ability to serve as single-cell protein and production platforms for fine chemicals from waste materials. We also introduce more Avant-Garde technologies that leverage the unique metabolisms of purple bacteria, including microbial electrosynthesis and co-culture. These technologies will be pivotal in our efforts to mitigate climate change and circularize the economy in the next two decades.</p><p><strong>One-sentence summary: </strong>Purple non-sulfur bacteria are utilized for a range of biotechnological applications, including the production of bio-energy, single cell protein, fertilizer, bioplastics, fine chemicals, in wastewater treatment and in novel applications like co-cultures and microbial electrosynthesis.</p>","PeriodicalId":16092,"journal":{"name":"Journal of Industrial Microbiology & Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11730080/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142895168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}