Journal of Industrial Microbiology & Biotechnology最新文献_第10页

Recent progress in adaptive laboratory evolution of industrial microorganisms. 工业微生物实验室适应性进化的最新进展。

IF 3.2 4区生物学

Journal of Industrial Microbiology & Biotechnology Pub Date : 2023-02-17 DOI: 10.1093/jimb/kuac023

Guanglu Wang, Qian Li, Zhan Zhang, Xianzhong Yin, Bingyang Wang, Xuepeng Yang

{"title":"Recent progress in adaptive laboratory evolution of industrial microorganisms.","authors":"Guanglu Wang, Qian Li, Zhan Zhang, Xianzhong Yin, Bingyang Wang, Xuepeng Yang","doi":"10.1093/jimb/kuac023","DOIUrl":"10.1093/jimb/kuac023","url":null,"abstract":"Adaptive laboratory evolution (ALE) is a technique for the selection of strains with better phenotypes by long-term culture under a specific selection pressure or growth environment. Because ALE does not require detailed knowledge of a variety of complex and interactive metabolic networks, and only needs to simulate natural environmental conditions in the laboratory to design a selection pressure, it has the advantages of broad adaptability, strong practicability, and more convenient transformation of strains. In addition, ALE provides a powerful method for studying the evolutionary forces that change the phenotype, performance, and stability of strains, resulting in more productive industrial strains with beneficial mutations. In recent years, ALE has been widely used in the activation of specific microbial metabolic pathways and phenotypic optimization, the efficient utilization of specific substrates, the optimization of tolerance to toxic substance, and the biosynthesis of target products, which is more conducive to the production of industrial strains with excellent phenotypic characteristics. In this paper, typical examples of ALE applications in the development of industrial strains and the research progress of this technology are reviewed, followed by a discussion of its development prospects.","PeriodicalId":16092,"journal":{"name":"Journal of Industrial Microbiology & Biotechnology","volume":"50 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2023-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/73/9d/kuac023.PMC9936214.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10805985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Strategic nutrient sourcing for biomanufacturing intensification. 强化生物制造的战略营养来源。

IF 3.4 4区生物学

Journal of Industrial Microbiology & Biotechnology Pub Date : 2023-02-17 DOI: 10.1093/jimb/kuad011

Kimia Noroozi Fashkhami, Laura R Jarboe

{"title":"Strategic nutrient sourcing for biomanufacturing intensification.","authors":"Kimia Noroozi Fashkhami, Laura R Jarboe","doi":"10.1093/jimb/kuad011","DOIUrl":"10.1093/jimb/kuad011","url":null,"abstract":"The successful design of economically viable bioprocesses can help to abate global dependence on petroleum, increase supply chain resilience, and add value to agriculture. Specifically, bioprocessing provides the opportunity to replace petrochemical production methods with biological methods and to develop novel bioproducts. Even though a vast range of chemicals can be biomanufactured, the constraints on economic viability, especially while competing with petrochemicals, are severe. There have been extensive gains in our ability to engineer microbes for improved production metrics and utilization of target carbon sources. The impact of growth medium composition on process cost and organism performance receives less attention in the literature than organism engineering efforts, with media optimization often being performed in proprietary settings. The widespread use of corn steep liquor as a nutrient source demonstrates the viability and importance of \"waste\" streams in biomanufacturing. There are other promising waste streams that can be used to increase the sustainability of biomanufacturing, such as the use of urea instead of fossil fuel-intensive ammonia and the use of struvite instead of contributing to the depletion of phosphate reserves. In this review, we discuss several process-specific optimizations of micronutrients that increased product titers by twofold or more. This practice of deliberate and thoughtful sourcing and adjustment of nutrients can substantially impact process metrics. Yet the mechanisms are rarely explored, making it difficult to generalize the results to other processes. In this review, we will discuss examples of nutrient sourcing and adjustment as a means of process improvement.One-sentence summary: The potential impact of nutrient adjustments on bioprocess performance, economics, and waste valorization is undervalued and largely undercharacterized.","PeriodicalId":16092,"journal":{"name":"Journal of Industrial Microbiology & Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2023-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/f7/99/kuad011.PMC10549214.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9527177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Mechanistic aspects of IPTG (isopropylthio-β-galactoside) transport across the cytoplasmic membrane of Escherichia coli-a rate limiting step in the induction of recombinant protein expression. IPTG（异丙基硫代-β-半乳糖苷）通过大肠杆菌质膜转运的机制方面——诱导重组蛋白表达的限速步骤。

IF 3.4 4区生物学

Journal of Industrial Microbiology & Biotechnology Pub Date : 2023-02-17 DOI: 10.1093/jimb/kuad034

Rodrigo G Simas, Adalberto Pessoa Junior, Paul F Long

{"title":"Mechanistic aspects of IPTG (isopropylthio-β-galactoside) transport across the cytoplasmic membrane of Escherichia coli-a rate limiting step in the induction of recombinant protein expression.","authors":"Rodrigo G Simas, Adalberto Pessoa Junior, Paul F Long","doi":"10.1093/jimb/kuad034","DOIUrl":"10.1093/jimb/kuad034","url":null,"abstract":"Coupling transcription of a cloned gene to the lac operon with induction by isopropylthio-β-galactoside (IPTG) has been a favoured approach for recombinant protein expression using Escherichia coli as a heterologous host for more than six decades. Despite a wealth of experimental data gleaned over this period, a quantitative relationship between extracellular IPTG concentration and consequent levels of recombinant protein expression remains surprisingly elusive across a broad spectrum of experimental conditions. This is because gene expression under lac operon regulation is tightly correlated with intracellular IPTG concentration due to allosteric regulation of the lac repressor protein (lacY). An in-silico mathematical model established that uptake of IPTG across the cytoplasmic membrane of E. coli by simple diffusion was negligible. Conversely, lacY mediated active transport was a rapid process, taking only some seconds for internal and external IPTG concentrations to equalize. Optimizing kcat and KM parameters by targeted mutation of the galactoside binding site in lacY could be a future strategy to improve the performance of recombinant protein expression. For example, if kcat were reduced whilst KM was increased, active transport of IPTG across the cytoplasmic membrane would be reduced, thereby lessening the metabolic burden on the cell and expediating accumulation of recombinant protein. The computational model described herein is made freely available and is amenable to optimize recombinant protein expression in other heterologous hosts.One-sentence summary: A computational model made freely available to optimize recombinant protein expression in Escherichia coli other heterologous hosts.","PeriodicalId":16092,"journal":{"name":"Journal of Industrial Microbiology & Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2023-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10639102/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41235973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The growth and metabolome of Saccharomyces uvarum in wine fermentations are strongly influenced by the route of nitrogen assimilation. 葡萄酒发酵过程中酿酒酵母的生长和代谢受到氮同化途径的强烈影响。

IF 3.4 4区生物学

Journal of Industrial Microbiology & Biotechnology Pub Date : 2023-02-13 DOI: 10.1093/jimb/kuac025

Angela Coral-Medina, John P Morrissey, Carole Camarasa

{"title":"The growth and metabolome of Saccharomyces uvarum in wine fermentations are strongly influenced by the route of nitrogen assimilation.","authors":"Angela Coral-Medina, John P Morrissey, Carole Camarasa","doi":"10.1093/jimb/kuac025","DOIUrl":"https://doi.org/10.1093/jimb/kuac025","url":null,"abstract":"Nitrogen is a critical nutrient in beverage fermentations, influencing fermentation performance and formation of compounds that affect organoleptic properties of the product. Traditionally, most commercial wine fermentations rely on Saccharomyces cerevisiae but the potential of alternative yeasts is increasingly recognised because of the possibility to deliver innovative products and process improvements. In this regard, Saccharomyces uvarum is an attractive non-traditional yeast that, while quite closely related to S. cerevisiae, displays a different fermentative and aromatic profile. Although S. uvarum is used in cider-making and in some winemaking, better knowledge of its physiology and metabolism is required if its full potential is to be realised. To address this gap, we performed a comparative analysis of the response of S. uvarum and S. cerevisiae to 13 different sources of nitrogen, assessing key parameters such as growth, fermentation performance, the production of central carbon metabolites and aroma volatile compounds. We observed that the two species differ in the production of acetate, succinate, medium-chain fatty acids, phenylethanol, phenylethyl acetate, and fusel/branched acids in ways that reflect different distribution of fluxes in the metabolic network. The integrated analysis revealed different patterns of yeast performance and activity linked to whether growth was on amino acids metabolised via the Ehrlich pathway or on amino acids and compounds assimilated through the central nitrogen core. This study highlights differences between the two yeasts and the importance that nitrogen metabolism can play in modulating the sensory profile of wine when using S. uvarum as the fermentative yeast.","PeriodicalId":16092,"journal":{"name":"Journal of Industrial Microbiology & Biotechnology","volume":"49 6","pages":""},"PeriodicalIF":3.4,"publicationDate":"2023-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/b8/a5/kuac025.PMC9923386.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10705195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Improvement of rimocidin production in Streptomyces rimosus M527 by reporter-guided mutation selection. 利用报告基因引导突变选择提高链霉菌M527的环莫西丁产量。

IF 3.4 4区生物学

Journal of Industrial Microbiology & Biotechnology Pub Date : 2023-02-13 DOI: 10.1093/jimb/kuac030

Yujie Jiang, Jinyao Zhang, Xinyi Huang, Zheng Ma, Yongyong Zhang, Andreas Bechthold, Xiaoping Yu

{"title":"Improvement of rimocidin production in Streptomyces rimosus M527 by reporter-guided mutation selection.","authors":"Yujie Jiang, Jinyao Zhang, Xinyi Huang, Zheng Ma, Yongyong Zhang, Andreas Bechthold, Xiaoping Yu","doi":"10.1093/jimb/kuac030","DOIUrl":"https://doi.org/10.1093/jimb/kuac030","url":null,"abstract":"In this study, we employed a reporter-guided mutation selection (RGMS) strategy to improve the rimocidin production of Streptomyces rimosus M527, which is based on a single-reporter plasmid pAN and atmospheric and room temperature plasma (ARTP). In plasmid pAN, PrimA, a native promoter of the loading module of rimocidin biosynthesis (RimA) was chosen as a target, and the kanamycin resistance gene (neo) under the control of PrimA was chosen as the reporter gene. The integrative plasmid pAN was introduced into the chromosome of S. rimosus M527 by conjugation to yield the initial strain S. rimosus M527-pAN. Subsequently, mutants of M527-pAN were generated by ARTP. 79 mutants were obtained in total, of which 67 mutants showed a higher level of kanamycin resistance (Kanr) than that of the initial strain M527-pAN. The majority of mutants exhibited a slight increase in rimocidin production compared with M527-pAN. Notably, 3 mutants, M527-pAN-S34, S38, and S52, which exhibited highest kanamycin resistance among all Kanr mutants, showed 34%, 52%, and 45% increase in rimocidin production compared with M527-pAN, respectively. Quantitative RT-PCR analysis revealed that the transcriptional levels of neo and rim genes were increased in mutants M527-pAN-S34, S38, and S52 compared with M527-pAN. These results confirmed that the RGMS approach was successful in improving the rimocidin production in S. rimosus M527.","PeriodicalId":16092,"journal":{"name":"Journal of Industrial Microbiology & Biotechnology","volume":"49 6","pages":""},"PeriodicalIF":3.4,"publicationDate":"2023-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/6a/37/kuac030.PMC9923380.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10758984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Co‑cultivation of anaerobic fungi with Clostridium acetobutylicum bolsters butyrate and butanol production from cellulose and lignocellulose. 厌氧真菌与乙酰丁酸梭菌的共培养促进了纤维素和木质纤维素生产丁酸盐和丁醇。

IF 3.4 4区生物学

Journal of Industrial Microbiology & Biotechnology Pub Date : 2023-02-13 DOI: 10.1093/jimb/kuac024

Jennifer L Brown, Matthew A Perisin, Candice L Swift, Marcus Benyamin, Sanchao Liu, Vasanth Singan, Yu Zhang, Emily Savage, Christa Pennacchio, Igor V Grigoriev, Michelle A O'Malley

{"title":"Co‑cultivation of anaerobic fungi with Clostridium acetobutylicum bolsters butyrate and butanol production from cellulose and lignocellulose.","authors":"Jennifer L Brown, Matthew A Perisin, Candice L Swift, Marcus Benyamin, Sanchao Liu, Vasanth Singan, Yu Zhang, Emily Savage, Christa Pennacchio, Igor V Grigoriev, Michelle A O'Malley","doi":"10.1093/jimb/kuac024","DOIUrl":"https://doi.org/10.1093/jimb/kuac024","url":null,"abstract":"A system for co-cultivation of anaerobic fungi with anaerobic bacteria was established based on lactate cross-feeding to produce butyrate and butanol from plant biomass. Several co-culture formulations were assembled that consisted of anaerobic fungi (Anaeromyces robustus, Neocallimastix californiae, or Caecomyces churrovis) with the bacterium Clostridium acetobutylicum. Co-cultures were grown simultaneously (e.g., 'one pot'), and compared to cultures where bacteria were cultured in fungal hydrolysate sequentially. Fungal hydrolysis of lignocellulose resulted in 7-11 mM amounts of glucose and xylose, as well as acetate, formate, ethanol, and lactate to support clostridial growth. Under these conditions, one-stage simultaneous co-culture of anaerobic fungi with C. acetobutylicum promoted the production of butyrate up to 30 mM. Alternatively, two-stage growth slightly promoted solventogenesis and elevated butanol levels (∼4-9 mM). Transcriptional regulation in the two-stage growth condition indicated that this cultivation method may decrease the time required to reach solventogenesis and induce the expression of cellulose-degrading genes in C. acetobutylicum due to relieved carbon-catabolite repression. Overall, this study demonstrates a proof of concept for biobutanol and bio-butyrate production from lignocellulose using an anaerobic fungal-bacterial co-culture system.","PeriodicalId":16092,"journal":{"name":"Journal of Industrial Microbiology & Biotechnology","volume":"49 6","pages":""},"PeriodicalIF":3.4,"publicationDate":"2023-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9923384/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10257713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

De novo Synthesis of 2-phenylethanol from Glucose by Metabolically Engineered Escherichia coli. 利用代谢工程大肠杆菌从葡萄糖中重新合成2-苯乙醇。

IF 3.4 4区生物学

Journal of Industrial Microbiology & Biotechnology Pub Date : 2023-02-13 DOI: 10.1093/jimb/kuac026

Guanglu Wang, Mengyuan Wang, Jinchu Yang, Qian Li, Nianqing Zhu, Lanxi Liu, Xianmei Hu, Xuepeng Yang

{"title":"De novo Synthesis of 2-phenylethanol from Glucose by Metabolically Engineered Escherichia coli.","authors":"Guanglu Wang, Mengyuan Wang, Jinchu Yang, Qian Li, Nianqing Zhu, Lanxi Liu, Xianmei Hu, Xuepeng Yang","doi":"10.1093/jimb/kuac026","DOIUrl":"https://doi.org/10.1093/jimb/kuac026","url":null,"abstract":"2-Phenylethanol (2- PE) is an aromatic alcohol with wide applications, but there is still no efficient microbial cell factory for 2-PE based on Escherichia coli. In this study, we constructed a metabolically engineered E. coli capable of de novo synthesis of 2-PE from glucose. Firstly, the heterologous styrene-derived and Ehrlich pathways were individually constructed in an L-Phe producer. The results showed that the Ehrlich pathway was better suited to the host than the styrene-derived pathway, resulting in a higher 2-PE titer of ∼0.76 ± 0.02 g/L after 72 h of shake flask fermentation. Furthermore, the phenylacetic acid synthase encoded by feaB was deleted to decrease the consumption of 2-phenylacetaldehyde, and the 2-PE titer increased to 1.75 ± 0.08 g/L. As phosphoenolpyruvate (PEP) is an important precursor for L-Phe synthesis, both the crr and pykF genes were knocked out, leading to ∼35% increase of the 2-PE titer, which reached 2.36 ± 0.06 g/L. Finally, a plasmid-free engineered strain was constructed based on the Ehrlich pathway by integrating multiple ARO10 cassettes (encoding phenylpyruvate decarboxylases) and overexpressing the yjgB gene. The engineered strain produced 2.28 ± 0.20 g/L of 2-PE with a yield of 0.076 g/g glucose and productivity of 0.048 g/L/h. To our best knowledge, this is the highest titer and productivity ever reported for the de novo synthesis of 2-PE in E. coli. In a 5-L fermenter, the 2-PE titer reached 2.15 g/L after 32 h of fermentation, suggesting that the strain has the potential to efficiently produce higher 2-PE titers following further fermentation optimization.","PeriodicalId":16092,"journal":{"name":"Journal of Industrial Microbiology & Biotechnology","volume":"49 6","pages":""},"PeriodicalIF":3.4,"publicationDate":"2023-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/0b/73/kuac026.PMC9923381.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10696454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A multi-plex protein expression system for production of complex enzyme formulations in Trichoderma reesei. 里氏木霉生产复合酶制剂的多重蛋白表达系统。

IF 3.4 4区生物学

Journal of Industrial Microbiology & Biotechnology Pub Date : 2023-02-13 DOI: 10.1093/jimb/kuac027

Venkataramanan Subramanian, Samuel J Farmer, Kelsey L Heiland, Kyle T Moore, Todd A Vander Wall, Weiman Sun, Yogesh B Chaudhari, Michael E Himmel, Stephen R Decker

{"title":"A multi-plex protein expression system for production of complex enzyme formulations in Trichoderma reesei.","authors":"Venkataramanan Subramanian, Samuel J Farmer, Kelsey L Heiland, Kyle T Moore, Todd A Vander Wall, Weiman Sun, Yogesh B Chaudhari, Michael E Himmel, Stephen R Decker","doi":"10.1093/jimb/kuac027","DOIUrl":"https://doi.org/10.1093/jimb/kuac027","url":null,"abstract":"Heterologous protein production has been challenging in the hyper-cellulolytic fungus, Trichoderma reesei as the species is known for poor transformation efficiency, low homologous recombination frequency, and marginal screening systems for the identification of successful transformants. We have applied the 2A-peptide multi-gene expression system to co-express four proteins, which include three cellulases: a cellobiohydrolase (CBH1), an endoglucanase (EG1), and a β-D-glucosidase (BGL1), as well as the enhanced green fluorescent protein (eGFP) marker protein. We designed a new chassis vector, pTrEno-4X-2A, for this work. Expression of these cellulase enzymes was confirmed by real-time quantitative reverse transcription PCR and immunoblot analysis. The activity of each cellulase was assessed using chromogenic substrates, which confirmed the functionality of the enzymes. Expression and activity of these enzymes were proportional to the level of eGFP fluorescence, thereby validating the reliability of this screening technique. An 18-fold differencein protein expression was observed between the first and third genes within the 2A-peptide construct. The availability of this new multi-gene expression and screening tool is expected to greatly impact multi-enzyme applications, such as the production of complex commercial enzyme formulations and metabolic pathway enzymes, especially those destined for cell-free applications.","PeriodicalId":16092,"journal":{"name":"Journal of Industrial Microbiology & Biotechnology","volume":"49 6","pages":""},"PeriodicalIF":3.4,"publicationDate":"2023-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9923369/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10700572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of nitrogen sources on the tolerance of Lacticaseibacillus rhamnosus to heat stress and oxidative stress. 氮源对鼠李糖乳杆菌耐热性和氧化性的影响。

IF 3.4 4区生物学

Journal of Industrial Microbiology & Biotechnology Pub Date : 2022-10-13 DOI: 10.1093/jimb/kuac020

Chenchen Zhang, Yuemei Han, Ya Gui, Yunchao Wa, Dawei Chen, Yujun Huang, Boxing Yin, Ruixia Gu

{"title":"Influence of nitrogen sources on the tolerance of Lacticaseibacillus rhamnosus to heat stress and oxidative stress.","authors":"Chenchen Zhang, Yuemei Han, Ya Gui, Yunchao Wa, Dawei Chen, Yujun Huang, Boxing Yin, Ruixia Gu","doi":"10.1093/jimb/kuac020","DOIUrl":"https://doi.org/10.1093/jimb/kuac020","url":null,"abstract":"It has been found that 32 genes related to nitrogen source metabolism in Lacticaseibacillus rhamnosus are downregulated under both heat stress and oxidative stress. In this study, the influence of different nitrogen sources within the growth medium on the tolerance of L. rhamnosus to heat stress and oxidative stress was investigated. Tryptone-free MRS was found to enhance the tolerance of L. rhamnosus hsryfm 1301 to heat stress and oxidative stress during the whole growth period, and this result was universal for all L. rhamnosus species analyzed. The strongest strengthening effect occurred when the OD600 value reached 2.0, at which the survival rates under heat stress and oxidative stress increased 130-fold and 40-fold, respectively. After supplementing phenylalanine, isoleucine, glutamate, valine, histidine, or tryptophan into the tryptone-free MRS, the tolerance of L. rhamnosus to heat stress and oxidative stress exhibited a sharp drop. The spray drying survival rate of L. rhamnosus hsryfm 1301 cultured in the tryptone-free MRS rose to 75% (from 30%), and the spray dried powder also performed better in the experimentally simulated gastrointestinal digestion. These results showed that decreasing the intake of amino acids is an important mechanism for L. rhamnosus to tolerate heat stress and oxidative stress. When L. rhamnosus is cultured for spray drying, the concentration of the nitrogen source's components should be an important consideration.","PeriodicalId":16092,"journal":{"name":"Journal of Industrial Microbiology & Biotechnology","volume":"49 5","pages":""},"PeriodicalIF":3.4,"publicationDate":"2022-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/7e/41/kuac020.PMC9559300.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33449256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Bioindustrial manufacturing readiness levels (BioMRLs) as a shared framework for measuring and communicating the maturity of bioproduct manufacturing processes. 生物工业制造准备水平(BioMRLs)作为衡量和交流生物产品制造过程成熟度的共享框架。

IF 3.4 4区生物学

Journal of Industrial Microbiology & Biotechnology Pub Date : 2022-10-13 DOI: 10.1093/jimb/kuac022

Michael J Smanski, Aristos Aristidou, Ryan Carruth, John Erickson, Mark Gordon, Sandeep B Kedia, Kelvin H Lee, Darcy Prather, John E Schiel, Heather Schultheisz, Thomas P Treynor, Steven L Evans, Douglas C Friedman, Melanie Tomczak

{"title":"Bioindustrial manufacturing readiness levels (BioMRLs) as a shared framework for measuring and communicating the maturity of bioproduct manufacturing processes.","authors":"Michael J Smanski, Aristos Aristidou, Ryan Carruth, John Erickson, Mark Gordon, Sandeep B Kedia, Kelvin H Lee, Darcy Prather, John E Schiel, Heather Schultheisz, Thomas P Treynor, Steven L Evans, Douglas C Friedman, Melanie Tomczak","doi":"10.1093/jimb/kuac022","DOIUrl":"https://doi.org/10.1093/jimb/kuac022","url":null,"abstract":"Readiness level (RL) frameworks such as technology readiness levels and manufacturing readiness levels describe the status of a technology/manufacturing process on its journey from initial conception to commercial deployment. More importantly, they provide a roadmap to guide technology development and scale-up from a ''totality of system'' approach. Commercialization risks associated with too narrowly focused R&D efforts are mitigated. RLs are defined abstractly so that they can apply to diverse industries and technology sectors. However, differences between technology sectors make necessary the definition of sector specific RL frameworks. Here, we describe bioindustrial manufacturing readiness levels (BioMRLs), a classification system specific to bioindustrial manufacturing. BioMRLs will give program managers, investors, scientists, and engineers a shared vocabulary for prioritizing goals and assessing risks in the development and commercialization of a bioindustrial manufacturing process.","PeriodicalId":16092,"journal":{"name":"Journal of Industrial Microbiology & Biotechnology","volume":"49 5","pages":""},"PeriodicalIF":3.4,"publicationDate":"2022-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9559305/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33479834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1