Journal of Industrial Microbiology & Biotechnology最新文献

Engineering Streptomyces coelicolor for heterologous expression of the thiopeptide GE2270A - a cautionary tale. 工程链霉菌异种表达硫肽GE2270A -一个警世故事。

IF 3.2 4区生物学

Journal of Industrial Microbiology & Biotechnology Pub Date : 2025-07-18 DOI: 10.1093/jimb/kuaf019

Francesco Del Carratore, Erik K R Hanko, Kamila Schmidt, Oksana Bilyk, Suhui Ye Huang, Marianna Iorio, Mercedes Pérez-Bonilla, Rosario Pérez-Redondo, Michelle Rudden, Emmanuele Severi, Arianna Tocchetti, Margherita Sosio, Emily J Johnson, Timothy Kirkwood, Dominic R Whittall, Alkisti Manousaki, Olga Genilloud, Antonio Rodríguez-García, Gavin H Thomas, Stefano Donadio, Rainer Breitling, Eriko Takano

{"title":"Engineering Streptomyces coelicolor for heterologous expression of the thiopeptide GE2270A - a cautionary tale.","authors":"Francesco Del Carratore, Erik K R Hanko, Kamila Schmidt, Oksana Bilyk, Suhui Ye Huang, Marianna Iorio, Mercedes Pérez-Bonilla, Rosario Pérez-Redondo, Michelle Rudden, Emmanuele Severi, Arianna Tocchetti, Margherita Sosio, Emily J Johnson, Timothy Kirkwood, Dominic R Whittall, Alkisti Manousaki, Olga Genilloud, Antonio Rodríguez-García, Gavin H Thomas, Stefano Donadio, Rainer Breitling, Eriko Takano","doi":"10.1093/jimb/kuaf019","DOIUrl":"https://doi.org/10.1093/jimb/kuaf019","url":null,"abstract":"The thiopeptide GE2270A is a clinically relevant, ribosomally synthesised and post-translationally modified peptide (RiPP) naturally produced by Planobispora rosea. Due to the genetically intractable nature of P. rosea, heterologous expression is considered a possible route to yield improvement. In this study, we focused on improving GE2270A production through heterologous expression of the biosynthetic gene cluster (BGC) in the model organism Streptomyces coelicolor M1146. A statistically significant yield improvement was obtained in the S. coelicolor system through the data-driven rational engineering of the BGC, including the introduction of additional copies of key biosynthetic and regulatory genes. However, despite our best effort, the highest production level observed in the strains generated in this study is 12 × lower than published titres achieved in the natural producer and 50 × lower than published titres obtained using Nonomuraea ATCC 39727 as expression host. These results suggest that, while using the most genetically amenable strain as host can be the right choice when exploring different BGC designs, the choice of the most suitable host has a major effect on the achievable yield and should be carefully considered. The analysis of the multi-omics data obtained in this study suggests an important role of PbtX in GE2270A biosynthesis and provides insights into the differences in production metabolic profiles between the different strains.","PeriodicalId":16092,"journal":{"name":"Journal of Industrial Microbiology & Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144659396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Beyond MRS Broth: A Soytone Medium towards affordable culturing of South African vaginal Lactobacillaceae isolates. 超越MRS肉汤：大豆培养基对南非阴道乳酸杆菌科分离株的可负担培养。

IF 3.2 4区生物学

Journal of Industrial Microbiology & Biotechnology Pub Date : 2025-07-14 DOI: 10.1093/jimb/kuaf021

Obakeng Luthando Jona, Marijke A Fagan-Endres, Anna-Ursula Happel, Brian Kullin, Jo-Ann S Passmore, Susan T L Harrison

{"title":"Beyond MRS Broth: A Soytone Medium towards affordable culturing of South African vaginal Lactobacillaceae isolates.","authors":"Obakeng Luthando Jona, Marijke A Fagan-Endres, Anna-Ursula Happel, Brian Kullin, Jo-Ann S Passmore, Susan T L Harrison","doi":"10.1093/jimb/kuaf021","DOIUrl":"https://doi.org/10.1093/jimb/kuaf021","url":null,"abstract":"This study assesses a plant-based Soytone Medium as an alternative to the animal-derived standard MRS Broth for the cultivation of Lactobacillaceae. The application focuses on five isolates that have shown probiotic potential for bacterial vaginosis treatment. Cultivation was performed in 300 mL bench-scale bioreactors, monitored for cell density, pH, lactate production and glucose consumption. The media's carbon and nitrogen concentrations and costing were quantified. Though the medium's carbon concentrations were identical, the Soytone Medium had a higher carbon-to-nitrogen ratio than MRS (8.1 vs 6.6). Four strains achieved higher cell densities and maximum specific growth rates in the Soytone Medium. The greatest benefit was shown for L. crispatus 70.6PA, which had a 45% higher final cell density. A cost analysis showed that the Soytone Medium was 44% cheaper than MRS Broth. It was thus confirmed that the proposed plant-based Soytone Medium is a viable and less expensive alternative for Lactobacillaceae cultures in which exposure to animal products was also avoided.","PeriodicalId":16092,"journal":{"name":"Journal of Industrial Microbiology & Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144626544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Towards engineering agaricomycete fungi for terpenoid production. 用于萜类化合物生产的工程菌丝体真菌。

IF 3.2 4区生物学

Journal of Industrial Microbiology & Biotechnology Pub Date : 2025-07-12 DOI: 10.1093/jimb/kuaf020

Riccardo Iacovelli, Dominik Mojzita, Peter Richard, Yvonne Nygård

{"title":"Towards engineering agaricomycete fungi for terpenoid production.","authors":"Riccardo Iacovelli, Dominik Mojzita, Peter Richard, Yvonne Nygård","doi":"10.1093/jimb/kuaf020","DOIUrl":"https://doi.org/10.1093/jimb/kuaf020","url":null,"abstract":"Since ancient times, humans have harnessed the vast metabolic abilities of fungi to produce food, beverages, and medicines. Biotechnology and genetic engineering have opened new avenues to tailor and enhance these abilities, transforming fungi into powerful industrial workhorses. In this minireview, we focus on the biotechnological potential of Agaricomycetes, a class of basidiomycete fungi that includes the so-called 'true' mushrooms. Although many species are widely used in the food sector, their broader potential in biotechnology remains largely untapped. These fungi naturally produce a diverse array of metabolites with promising applications across various industries. Here, we highlight their ability to synthesize a wide range of terpenoids, many unique to this taxon, and we present recent advancements in genomics and genetic engineering tools developed for Agaricomycetes. We anticipate that continued progress in tailored genetic engineering tools and improved cultivation technologies will facilitate the establishment of these fungi as robust cell factories for producing valuable terpenoids, with significant contributions to the food, biotech, and pharmaceutical sectors.","PeriodicalId":16092,"journal":{"name":"Journal of Industrial Microbiology & Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144618609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome-mining-guided discovery of coumarubrin: A novel aminocoumarin-substituted rubromycin antibiotic. 基因组挖掘引导下香豆素的发现：一种新的氨基香豆素取代红霉素抗生素。

IF 3.2 4区生物学

Journal of Industrial Microbiology & Biotechnology Pub Date : 2025-07-04 DOI: 10.1093/jimb/kuaf018

Heiner G Weddeling, Sven T Sowa, Selina Bosshardt, Lukas Schwimbersky, Malik Rakhmanov, Robin Teufel

{"title":"Genome-mining-guided discovery of coumarubrin: A novel aminocoumarin-substituted rubromycin antibiotic.","authors":"Heiner G Weddeling, Sven T Sowa, Selina Bosshardt, Lukas Schwimbersky, Malik Rakhmanov, Robin Teufel","doi":"10.1093/jimb/kuaf018","DOIUrl":"https://doi.org/10.1093/jimb/kuaf018","url":null,"abstract":"Rubromycins are bacterial aromatic polyketides containing a hallmark spiroketal pharmacophore produced by type II polyketide synthases and accessory enzymes. They generally display cytotoxic and antimicrobial properties, frequently disrupting cellular processes and proteins associated with nucleic acids, such as DNA helicase or telomerase. Among the known rubromycin congeners, hyaluromycin stands out due to an 2-amino-3-hydroxycyclopent-2-enone (C5N) substitution that is presumably installed by an amide bond synthetase (ABS). Here, we used bioinformatic analysis to identify uncharacterized biosynthetic gene clusters (BGCs) and potential rubromycin producer strains encoding putative ABSs but lacking the enzymes responsible for C5N formation, suggesting potentially novel substituents. One of these strains, Lentzea tibetensis, was successfully cultivated and confirmed to produce a previously undescribed aminocoumarin-substituted rubromycin polyketide, named coumarubrin, as verified by HRMS and comprehensive NMR spectroscopy. ECD spectroscopy indicates an absolute configuration identical to that of previously characterized rubromycins, while first bioactivity assays demonstrated potent inhibitory activity against Gram-positive bacteria.","PeriodicalId":16092,"journal":{"name":"Journal of Industrial Microbiology & Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144560282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Finding of the positive impact of glucose on the production of indican over indigo in engineered Escherichia coli. 在工程大肠杆菌中发现葡萄糖对靛蓝生产的积极影响。

IF 3.2 4区生物学

Journal of Industrial Microbiology & Biotechnology Pub Date : 2024-12-31 DOI: 10.1093/jimb/kuae048

Hyun Jin Kim, Yeda Lee, Yuni Shin, Suhye Choi, Jinok Oh, Suwon Kim, Jungoh Ahn, Kwon-Young Choi, Jeong Chan Joo, Shashi Kant Bhatia, Yung-Hun Yang

{"title":"Finding of the positive impact of glucose on the production of indican over indigo in engineered Escherichia coli.","authors":"Hyun Jin Kim, Yeda Lee, Yuni Shin, Suhye Choi, Jinok Oh, Suwon Kim, Jungoh Ahn, Kwon-Young Choi, Jeong Chan Joo, Shashi Kant Bhatia, Yung-Hun Yang","doi":"10.1093/jimb/kuae048","DOIUrl":"10.1093/jimb/kuae048","url":null,"abstract":"Indigo is a plant-based natural blue dye that can be produced via chemical synthesis and biological pathways. However, the toxic reduction processes and intracellular production of indigo through microbial metabolism are often limited by insolubility of indigo and complex downstream processing, causing environmental issues in the dyeing processes. Additionally, indican, a precursor of indigo with a glucose moiety, is highly soluble and can be easily converted into indoxyl by β-glucosidase, forming indigo under mild conditions. We constructed an indican-producing strain Escherichia coli BL21 HI201 by introducing a UDP-glycosyltransferase (ugt) into an indoxyl production system containing tryptophanse (tnaA) and flavin-containing monooxygenase (FMO) genes, enabling conversion of tryptophan into indican. Testing of the effect by various carbon sources suggested that glucose is one of the major factors affecting the ratio of indigo to indican, and increase in glucose concentration to more than 1.5% could produce sole indican without indigo. Under optimal conditions, E. coli BL21 HI201 biosynthesized 5.65 mM indican from tryptophan. Additionally, after deletion of various β-glucosidase genes, the bglA knockout strain E. coli BL21 HI204 produced more indican, achieving 6.79 mM after 24 hr of cultivation. This study demonstrated the strategic production of indican through the installation of a production system, deletion of a byproduct pathway, and control of glucose concentration.One-sentence summary: This paper demonstrates the strategic enhancement of indican production in genetically engineered Escherichia coli BL21 by optimizing metabolic pathways and controlling glucose concentrations.","PeriodicalId":16092,"journal":{"name":"Journal of Industrial Microbiology & Biotechnology","volume":"52 ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11947661/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143730374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced amino acid biosynthesis in Phaffia rhodozyma via herbicide-induced selection. 除草剂诱导选择促进法菲酵母氨基酸生物合成。

IF 3.2 4区生物学

Journal of Industrial Microbiology & Biotechnology Pub Date : 2024-12-31 DOI: 10.1093/jimb/kuaf011

Svetlana Raita, Iveta Kuzmika, Taras Mika, Zane Geiba, Kriss Spalvins

{"title":"Enhanced amino acid biosynthesis in Phaffia rhodozyma via herbicide-induced selection.","authors":"Svetlana Raita, Iveta Kuzmika, Taras Mika, Zane Geiba, Kriss Spalvins","doi":"10.1093/jimb/kuaf011","DOIUrl":"10.1093/jimb/kuaf011","url":null,"abstract":"According to the Food and Agricultural Organisation 2024 statement, developing single-cell protein technology is important to reduce the burden on conventional feed protein production sectors. In this regard, improved commercial strains rich in amino acids, especially Lys and Met, may provide a sustainable alternative source of protein in aquaculture diets. The developed and laboratory-validated methodology for creating protein-synthesizing mutants will strengthen the competitiveness of SCP production technology. The present work provides unique results on improving the protein-producing properties of wild-type Phaffia rhodozyma DSM 5626 by mutagenesis and screening on herbicide-containing medium as a selective agent for amino acid biosynthesis inhibition. Inhibitory concentrations of pure herbicide actives were determined for S-(2-aminoethyl)-L-cysteine (AEC) hydrochloride and glufosinate-ammonium (GA) for complete inhibition and strong inhibition of the DSM 5626 strain. GA at a concentration of 50 mM and 100 mM and AEC at 0.5 mM and 2.5 mM were chosen for mutant selection after chemical mutagenesis. The use of herbicides resulted in the selection of mutants with significantly improved synthesis of Met and Lys. Specifically, mutants GA6/4 and GA7/5 exhibited 37% and 26% higher Met levels, respectively, while GA6/3 had a 14% increase in Lys compared to the wild-type strain. The AEC3/9 mutant demonstrated a 35% increase in Met, 24% in Lys, 8% in Ile, and 6% in Phe, underscoring the efficacy of this screening approach in enhancing essential amino acid content. The protein quality parameters essential amino acid index and amino acid score of these mutants became higher compared with commercial strains of SCP yeast such as C. utilis, S. cerevisiae, K. marxianus, etc.One-sentence summary: Mutagenesis combined with selective screening using herbicides is an effective approach to enhancing amino acid biosynthesis in yeast.","PeriodicalId":16092,"journal":{"name":"Journal of Industrial Microbiology & Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12107244/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144021174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent advancement of glucosamine and N-acetyl glucosamine production using microorganisms: A review. 微生物生产氨基葡萄糖和n -乙酰氨基葡萄糖的研究进展

IF 3.2 4区生物学

Journal of Industrial Microbiology & Biotechnology Pub Date : 2024-12-31 DOI: 10.1093/jimb/kuaf014

Anica Tasnim Protity, Shengde Zhou

{"title":"Recent advancement of glucosamine and N-acetyl glucosamine production using microorganisms: A review.","authors":"Anica Tasnim Protity, Shengde Zhou","doi":"10.1093/jimb/kuaf014","DOIUrl":"10.1093/jimb/kuaf014","url":null,"abstract":"Glucosamine (GlcN) and GlcN-based supplements, e.g. glucosamine hydrochloride, glucosamine sulfate, and N-acetyl glucosamine (GlcNAc), provide symptomatic relief to osteoarthritis patients and have been used as one of the most popular nutraceuticals. To meet the increasing demands, scientists have explored cost-effective methods for GlcN and GlcNAc production using low-cost raw materials such as seafood waste. However, the commercially available GlcN and GlcNAc production methods are environmentally harmful because of the use of toxic reagents. Moreover, the raw material used might be unsafe for consumers with shrimp allergies. On the other hand, bio-based GlcN production is gaining popularity because of its eco-friendly production approach and optimum reaction conditions. In this mini-review, we will discuss the recent developments to produce GlcN and GlcNAc through (1) the chemical and enzyme-mediated approaches of crude chitin hydrolysis, primarily obtained from shrimp and crabs; (2) the whole cell-based systems for fungal derived chitin bio-transformation and fungal fermentation; and (3) the metabolic engineering and the adaptive evolution based microbial biocatalyst for a balanced cell growth and optimal production of GlcN and GlcNAc. One-Sentence Summary: This article summarizes the mechanism of glucosamine and N-acetyl glucosamine production using bacteria, fungi, and chemical processes.","PeriodicalId":16092,"journal":{"name":"Journal of Industrial Microbiology & Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12168751/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144159638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Production of extracellular L-arginase by Alcaligenes aquatilis BC2 isolated from soda lakes (Lake Chitu) of Ethiopia. 埃塞俄比亚奇图湖Alcaligenes aquatilis BC2产胞外L-精氨酸酶的研究。

IF 3.2 4区生物学

Journal of Industrial Microbiology & Biotechnology Pub Date : 2024-12-31 DOI: 10.1093/jimb/kuaf017

Birhan Getie Assega, Kefyalew Ayalew Getahun, Tamene Milkessa, Tsehayneh Geremew Yohannes, Feleke Moges, Mulugeta Aemero, Berhanu Andualem

{"title":"Production of extracellular L-arginase by Alcaligenes aquatilis BC2 isolated from soda lakes (Lake Chitu) of Ethiopia.","authors":"Birhan Getie Assega, Kefyalew Ayalew Getahun, Tamene Milkessa, Tsehayneh Geremew Yohannes, Feleke Moges, Mulugeta Aemero, Berhanu Andualem","doi":"10.1093/jimb/kuaf017","DOIUrl":"10.1093/jimb/kuaf017","url":null,"abstract":"L-Arginase is a therapeutic enzyme that hydrolyzes L-arginine to ornithine and urea. The L-arginase extracted from bacteria has an anticancer activity by causing starvation of nutrients for cancer cells. This study aimed to screen and characterize L-arginase-producing bacteria and to optimize different factors influencing L-arginase production. Isolation and primary screening were carried out by using mineral arginine agar media using phenol red as an indicator. Molecular identification of the isolates was employed by using 16S ribosomal RNA sequencing and phylogenetic tree construction. L-Arginase assay by colorimetric method was carried out to measure the amount of urea liberated from the hydrolysis of L-arginine for quantitative screening. From 31 water samples, 102 colonies were isolated, and those colonies that convert the media to pink were selected as arginase-producing bacteria. 7 isolates were screened from qualitative screening method. Based on quantitative screening, the highest L-arginase was produced from bacteria Alcaligenes aquatilis BC2 (92.46 ± 0.19 U/ml) followed by Paenalcaligenes suwonensis BCW8 (59.29 ± 0.66 U/ml). Following their mean difference, isolate BC2 was selected for further optimization process of 8 parameters. After optimization, the isolate shows the maximum (163.85 U/ml) enzyme activity. The result of this study implies that novel bacteria were isolated from soda lakes that produce a considerable amount of L-arginase, which can be used as a promising anticancer activity. One-Sentence Summary: This study successfully isolated and characterized a novel L-arginase-producing bacterium, Alcaligenes aquatilis BC2, from Ethiopian soda lakes and optimized its enzyme production parameters for potential anticancer applications.","PeriodicalId":16092,"journal":{"name":"Journal of Industrial Microbiology & Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12259280/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144505960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biocatalytic diversification of abietic acid in Streptomyces. 链霉菌中枞酸的生物催化多样化。

IF 3.2 4区生物学

Journal of Industrial Microbiology & Biotechnology Pub Date : 2024-12-31 DOI: 10.1093/jimb/kuaf003

Caitlin A McCadden, Tyler A Alsup, Ion Ghiviriga, Jeffrey D Rudolf

{"title":"Biocatalytic diversification of abietic acid in Streptomyces.","authors":"Caitlin A McCadden, Tyler A Alsup, Ion Ghiviriga, Jeffrey D Rudolf","doi":"10.1093/jimb/kuaf003","DOIUrl":"10.1093/jimb/kuaf003","url":null,"abstract":"Biocatalysis provides access to synthetically challenging molecules and commercially and pharmaceutically relevant natural product analogs while adhering to principles of green chemistry. Cytochromes P450 (P450s) are among the most superlative and versatile oxidative enzymes found in nature and are desired regio- and stereoselective biocatalysts, particularly for structurally complex hydrocarbon skeletons. We used 10 genome-sequenced Streptomyces strains, selected based on their preponderance of P450s, to biotransform the bioactive diterpenoid abietic acid. We isolated and structurally characterized seven oxidized abietic acid derivatives from three different strains, including four products that are new bacterial biotransformants or enzymatic products. Oxidations (hydroxylation, dehydrogenation, and aromatization) were seen on both the B and C rings of abietic acid and five products had multiple modifications. Notable conversions observed in the study were that of abietic acid to 15-hydroxy-7-oxo-8,11,13-abietatrien-18-oic acid, 7, which involves multiple hydroxylation reactions and dehydrogenation. The findings from this study will lead to identifying P450s or other enzymes that may act as general biocatalysts to modify abietanes and other labdane-type diterpenoid skeletons.One-sentence summary: Genome-guided biotransformation of the bioactive diterpenoid abietic acid in Streptomyces yielded seven oxidized derivatives including four that have not been previously seen from bacteria.","PeriodicalId":16092,"journal":{"name":"Journal of Industrial Microbiology & Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11812575/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143065574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An overview of key industrial product citric acid production by Aspergillus niger and its application. 黑曲霉生产关键工业产品柠檬酸及其应用综述。

IF 3.2 4区生物学

Journal of Industrial Microbiology & Biotechnology Pub Date : 2024-12-31 DOI: 10.1093/jimb/kuaf007

Ambreen Latif, Noor Hassan, Hazrat Ali, Muhammad Bilal Khan Niazi, Zaib Jahan, Iqra Latif Ghuman, Farwa Hassan, Anam Saqib

{"title":"An overview of key industrial product citric acid production by Aspergillus niger and its application.","authors":"Ambreen Latif, Noor Hassan, Hazrat Ali, Muhammad Bilal Khan Niazi, Zaib Jahan, Iqra Latif Ghuman, Farwa Hassan, Anam Saqib","doi":"10.1093/jimb/kuaf007","DOIUrl":"10.1093/jimb/kuaf007","url":null,"abstract":"Citric acid possesses high economic value and is considered as the world's largest consumed organic acid in numerous industries. Citric acid applications range from food to beverage industries, pharmaceuticals, cosmetics, and the environment. It is mostly produced by microbial fermentation, but Aspergillus niger is considered as the main workhorse for large-scale production of citric acid. In the current review, special devotion has been made toward addressing the latest and innovative literature related to production of citric acid by A. niger. The review article discusses A. niger historical involvement in citric acid production, fermentation technologies, molecular biology, biosynthesis, accumulation of citric acid, methods for enhanced production of citric acid, different operational factors also influencing citric acid production, and various techniques used for citric acid recovery. Also, copious biotechnological applications of citric acid are summarized for a fundamental comprehension of the subject and its critical role in diverse fields of industries.One-sentence summary: This review describes the historical role of Aspergillus niger in the production of citric acid, fermentation technologies, molecular biology, techniques for increased citric acid production, and other physical and chemical variables influencing the production of citric acid.","PeriodicalId":16092,"journal":{"name":"Journal of Industrial Microbiology & Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11956825/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143742898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0