Journal of Industrial Microbiology & Biotechnology最新文献

{"title":"Metabolic engineering of Escherichia coli for resveratrol production using food-grade D-xylose as a carbon source.","authors":"Huy Quang Nguyen, Hoa An Thi Nguyen, Luan Luong Chu","doi":"10.1093/jimb/kuag017","DOIUrl":"10.1093/jimb/kuag017","url":null,"abstract":"<p><p>Resveratrol is a high-value polyphenolic compound widely utilized in nutraceutical, cosmetic, and pharmaceutical applications. However, most microbial production systems rely on glucose as the primary carbon source, which limits flexibility for integrating alternative and renewable feedstocks. In this study, we developed an engineered Escherichia coli platform to investigate resveratrol biosynthesis under xylose-supporting conditions, using food-grade D-xylose as the carbon source. A heterologous pathway consisting of Populus tomentosa 4-coumarate: CoA ligase (Pt4CL) and Arachis hypogaea stilbene synthase (AhSTS) was introduced to convert externally supplied p-coumaric acid (PCA) into resveratrol. To improve precursor availability, intracellular malonyl-CoA supply was enhanced by introducing matB and matC from Streptomyces coelicolor A3(2) and overexpressing the acetyl-CoA carboxylase complex (ACC) from E. coli. Xylose assimilation was further strengthened by expressing xylE, xylA, and xylB, while carbon catabolite repression was alleviated using CRISPR interference (CRISPRi) targeting the glucose transporter gene ptsG. Under shake-flask conditions, the engineered strain produced up to 23.9 mg/L resveratrol from food-grade D-xylose, accompanied by near-complete xylose consumption and 93%-94% precursor conversion. This corresponded to an overall fermentation yield of approximately 12.5 mg resveratrol per g xylose consumed. Similar titers (27 mg/L) were obtained in a 5-L bioreactor, indicating stable pathway performance under controlled fermentation conditions. Overall, these results show that E. coli can be engineered to support efficient precursor-to-product conversion under xylose-supported conditions, providing a useful proof-of-concept framework for integrating alternative carbon sources into microbial production platforms for aromatic compounds. One sentence summary An engineered Escherichia coli system integrates xylose utilization, malonyl-CoA pathway optimization, and CRISPRi regulation to support resveratrol biosynthesis under xylose-supported conditions.</p>","PeriodicalId":16092,"journal":{"name":"Journal of Industrial Microbiology & Biotechnology","volume":" ","pages":""},"PeriodicalIF":4.5,"publicationDate":"2026-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13358167/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148375979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dynamics of contaminant microbes in bioethanol production from sugarcane.","authors":"Ana Laura R Romano, Natalia Coutouné, Artur Rego-Costa, Michael M Desai, Marcelo F Carazzolle, Andreas K Gombert","doi":"10.1093/jimb/kuag020","DOIUrl":"10.1093/jimb/kuag020","url":null,"abstract":"<p><p>The dynamics and impact of microbial contaminants in industrial sugarcane bioethanol production in Brazil were investigated through a 2-year metagenomic study across two biorefineries. Shotgun metagenomic sequencing revealed that temporal shifts in the contaminant microbiome dynamics within production seasons were more pronounced than inter-annual or inter-mill variations. While Saccharomyces spp. dominated, bacterial communities, primarily within the Firmicutes phylum and dominated by the genera Lactobacillus, Limosilactobacillus, and Bacillus, exhibited dynamic changes. Correlation analyses with industrial process parameters revealed a complex interplay: lower Lactobacillus levels in one mill were associated with increased ethanol yield, whereas higher levels in another mill correlated with reduced yeast viability and increased flocculation. The presence of Limosilactobacillus was linked to decreased yeast viability, whereas Bacillus showed potential for inhibiting both Lactobacillus and Limosilactobacillus. These findings highlight the nuanced and species-specific impacts of bacterial contaminants on bioethanol production, underscoring the need for strain-level functional studies and targeted interventions to optimize fermentation efficiency and stability in industrial settings. One-Sentence Summary The dynamics of contaminant bacterial populations during fuel ethanol production from sugarcane was unrevealed in two biorefineries and in two consecutive production years using shotgun metagenomics.</p>","PeriodicalId":16092,"journal":{"name":"Journal of Industrial Microbiology & Biotechnology","volume":" ","pages":""},"PeriodicalIF":4.5,"publicationDate":"2026-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13615390/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148725938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protoplast fusion as a strategy to increase ploidy in Rhodotorula toruloides for strain development.","authors":"Daiane D Lopes, Bruce S Dien, Ronald E Hector, Stephanie R Thompson, Vijay Singh, Sujit S Jagtap, Nancy N Nichols, Christopher V Rao, Christopher Skory, Patricia J Slininger","doi":"10.1093/jimb/kuaf037","DOIUrl":"10.1093/jimb/kuaf037","url":null,"abstract":"<p><p>Rhodotorula toruloides is a red oleaginous yeast with growing commercial interest because of its hardiness and exceptional lipid production capacity. Because it is a basidiomycete yeast with a complex life cycle, many of the classical breeding methods used with ascomycetes are unavailable for strain improvement. However, we have been able to construct polyploid yeast by fusing protoplasts of parents with the same mating type. Fusing of Y-6985 (A2) and Y-48190 (A2), which had been transformed with complementary antibiotic markers, led to the recovery of two diploids and one triploid. The stability of the fusion yeasts was tested by plating them on non-selective medium after several growth cycles under antibiotics and then testing five colonies per strain for nuclear DNA contents using flow cytometry and standard cell cycle analysis: the triploid and one diploid were stable. Fusants inherited their mitochondria from a single parent, which was demonstrated using restriction fragment length polymorphism (RFLP) of mitochondrial DNA. The phenotypic properties of the parents and fusants were compared in glucose fed-batch bioreactor studies and cellulosic sugar batch cultures. The final lipid titers for the fed-batch cultures were 24.9-39.7 g/L with Y-6985 and the diploid and triploid performing the best and worst, respectively. The fusants demonstrated intermediate hardiness for growth on hydrolysate prepared with dilute-acid pretreated switchgrass and were outperformed by Y-48190. Unlike one of the haploid parents, the fusants grew in 70% v/v concentrated hydrolysate. However, they did not grow as fast as the other haploid. In this study, a modernized protoplast fusion method is resurrected a useful tool for strain development in this yeast, which is complementary with other available methods.</p>","PeriodicalId":16092,"journal":{"name":"Journal of Industrial Microbiology & Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12781891/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145708066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Citric acid treatment of Tetradesmus obliquus biomass reduces dry matter loss in handling, queuing, and long-term storage, while stimulating auto-fermentative production of succinic acid.","authors":"Bradley D Wahlen, Chelsea C St Germain, Lynn M Wendt, John McGowen, Yaqi You","doi":"10.1093/jimb/kuag008","DOIUrl":"10.1093/jimb/kuag008","url":null,"abstract":"<p><p>Post-harvest algae biomass is prone to degradation, resulting in mass loss and compositional changes, and preservation is vital to economic viability of algal products. Effective storage solutions are needed to mitigate for seasonal productivity variations (long-term storage) and to keep post-harvest biomass stable until processing. Ensiling has emerged as a long-term storage solution capable of preserving biomass up to 6 months with little loss without the energy demands of drying. Organic acids produced during ensiling lower biomass pH and prevent growth of degradative bacteria such as Clostridia. However, losses are front-loaded with a majority occurring within the first week before organic acids can accumulate. Currently, there is no information on the stability of algae biomass within the first 24 hr post-harvest or methods available to ensure stability during this period. Freshly harvested Tetradesmus obliquus UTEX 393 biomass was stored in three conditions: ambient atmosphere, anaerobic atmosphere without treatment, and anaerobic atmosphere with citric acid amendment. Citric acid treatment limited mass loss to 1% after 28 days, while untreated biomass experienced 4% mass loss after just 4 hr and 18% mass loss after 4 weeks. The carbohydrate fraction was most affected, with minimal changes to the elemental composition of biomass across treatments. Bacilli bacteria, including lactic acid bacteria, increased in abundance under all storage conditions. Untreated biomass showed a rise in Clostridia, but none were found in citric acid-treated biomass. After 28 days, organic acid composition differed significantly among treatments, with succinic acid being accumulated to 30% of dry cellular weight in citric acid treated UTEX 393 biomass. Citric acid treatment effectively mitigates biomass loss and, surprisingly, promotes substantial production of succinic acid. The unexpected autofermentation of UTEX 393 biomass to a versatile intermediate chemical such as succinic acid at high titers with minimal energy input could contribute to the economic viability of algae cultivation for fuels and chemicals. One-Sentence Summary  Tetradesmus obliquus biomass is susceptible to degradation immediately after harvest; citric acid treatment preserves biomass while stimulating succinic acid accumulation.</p>","PeriodicalId":16092,"journal":{"name":"Journal of Industrial Microbiology & Biotechnology","volume":" ","pages":""},"PeriodicalIF":4.5,"publicationDate":"2026-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13049484/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147458164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hybrid modeling for industrial fermentation processes with an \"Intra-Batch Experimental Design\".","authors":"Marc Lemperle, Pedram Ramin, Julian Kager, Benny Cassells, Stuart Stocks, Krist V Gernaey","doi":"10.1093/jimb/kuag014","DOIUrl":"10.1093/jimb/kuag014","url":null,"abstract":"<p><p>Successful development of a predictive digital twin or digital shadow enabling improved batch planning and process optimization of industrial fungal fermentation relies on the fidelity of oxygen transfer rate modeling. Such models depend, among other factors, on viscosity. Traditional process models use mechanistic approaches to describe the apparent viscosity but face challenges due to its inherent complex behavior, requiring many assumptions and often relying on tedious offline rheological measurements. This article presents a novel model development framework, which significantly reduces associated experimental costs and eliminates the need for offline rheological measurement. First, a method for developing a model for the oxygen mass transfer coefficient (kLa) at pilot scale is presented, reducing experimental effort from nine to two fermentations while achieving an R² of 0.92 with online data compared to an R² of 0.67 with offline data. Alongside the mechanistic model, which links biological yields to the fungal growth rate, three machine learning algorithms were evaluated as a data-driven soft sensor for predicting online viscosity across different strains and scales. The outcome is a hybrid model that requires less manual lab work for its development while predicting the dynamics of six pilot-scale fermentations under a variety of operating conditions with a modest improvement in accuracy. Results emphasize the importance of integrating online sensor technologies into mathematical model development and highlight their role in advancing data-driven methods. By lowering the costs and efforts of model development, this study contributes to the long-term vision of automated model development for industrial applications. One-sentence summary This study presents a novel hybrid modeling approach that minimizes experimental efforts by replacing offline viscosity measurements with online dynamic viscosity data to predict oxygen transfer for industrial fungal fermentation processes at a 550 L pilot scale, enabling more time, and cost-efficient bioprocess model development.</p>","PeriodicalId":16092,"journal":{"name":"Journal of Industrial Microbiology & Biotechnology","volume":" ","pages":""},"PeriodicalIF":4.5,"publicationDate":"2026-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13278493/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148149682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Kratom medium-chain dehydrogenase/reductase enzymes exhibit broad substrate and cofactor promiscuity.","authors":"Anna Koganitsky, Dong Oh Han, Vivian Liu, Amir Alwali, Sijin Li","doi":"10.1093/jimb/kuag012","DOIUrl":"10.1093/jimb/kuag012","url":null,"abstract":"<p><p>Medium-chain alcohol dehydrogenases/reductases (MDRs) are crucial to the biosynthesis of plant monoterpene indole alkaloids (MIAs), yet studies have focused solely on their catalytic activities with strictosidine aglycone derivatives and NADPH as the redox cofactor. Here, we combined computational and experimental approaches to characterize the substrate and cofactor promiscuity of five MDRs recently identified in the medicinal plant kratom, including MsMDR4, MsMDR11, MsTHAS (tetrahydroalstonine synthase), MsDCS1 (dihydrocorynantheine synthase), and MsHYS (heteroyohimbine synthase). In vitro and yeast-based biochemical analyses showed that MsMDR4, MsMDR11, and MsTHAS catalyze the aldehyde reduction for cinnamaldehyde and 8-oxogeraniol, while MsMDR11 and MsTHAS also catalyze the alcohol oxidation for cinnamyl alcohol and 8-hydroxygeraniol. In particular, MsMDR11 exhibits 8-hydroxygeraniol oxidoreductase activity, producing 8-oxogeranial, an upstream intermediate in the strictosidine pathway. Additionally, all five enzymes can accept NADH/NAD + as redox cofactors, though with decreased productivity. Swapping the reducing cofactor from NADPH to NADH further alters the distribution of MIAs produced by MsDCS1 and MsHYS. Together, kratom MDRs exhibit great plasticity and potential for future enzyme engineering. One-sentence summary Computational and biochemical analyses revealed that previously identified kratom medium-chain dehydrogenase/reductases exhibit broad substrate and cofactor promiscuity.</p>","PeriodicalId":16092,"journal":{"name":"Journal of Industrial Microbiology & Biotechnology","volume":" ","pages":""},"PeriodicalIF":4.5,"publicationDate":"2026-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13146661/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147773712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Field-validated microbial deodorant reduces hazardous gas exposure and improves air quality in rural household toilets.","authors":"Shangyi Shu, Yujun Shen, Lage Wuri, Yiman Jia, Ao Li, Jingtao Ding, Huihui Wang","doi":"10.1093/jimb/kuag024","DOIUrl":"10.1093/jimb/kuag024","url":null,"abstract":"<p><p>Malodorous emissions from rural sanitation systems pose a direct environmental health concern. This study developed a composite microbial deodorant (CMD) using five bacterial strains isolated from activated sludge, combined in an optimal 1:1:1:1:1 ratio determined by orthogonal testing, to mitigate emissions of NH₃ and H₂S-key hazardous and odorous pollutants. In laboratory simulations, the CMD reduced NH₃ and H₂S by 82.1% and 86.1%, respectively. A pivotal 6-month field evaluation in three common types of rural household toilets demonstrated sustained, real-world efficacy, with peak removal rates of 79.43% for NH₃ and 80.83% for H₂S. The intervention also conserved nitrogen, reducing total nitrogen loss by 14.8%. Metagenomic analysis revealed the mechanistic basis: the CMD reshaped microbial metabolic pathways by upregulating genes for nitrification (hao) and sulfur oxidation (sqr), while suppressing reductive genes (nif, aprA), thus curbing emissions at source. This work provides robust field evidence that an in situ microbial strategy can effectively and sustainably improve household air quality in rural settings, offering a practical, low-cost, and eco-friendly solution for in situ odor control in rural sanitation- a critical step for sustaining the health benefits of the ''Toilet Revolution'' and improving rural living environments. One-Sentence Summary A composite microbial deodorant effectively reduces ammonia and hydrogen sulfide emissions and improves air quality in rural household toilets through 6-month field validation, providing an eco-friendly and low-cost odor control solution for rural sanitation.</p>","PeriodicalId":16092,"journal":{"name":"Journal of Industrial Microbiology & Biotechnology","volume":"53 ","pages":""},"PeriodicalIF":4.5,"publicationDate":"2026-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13662806/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149181794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multicriteria evaluation of ethylene glycol assimilation pathways.","authors":"Michelle Feigis, Radhakrishnan Mahadevan","doi":"10.1093/jimb/kuag007","DOIUrl":"10.1093/jimb/kuag007","url":null,"abstract":"<p><p>Biomanufacturing can play a pivotal role in the transition away from fossil fuel dependence for the production of chemicals and fuels. There is growing interest in inexpensive alternative bioproduction feedstocks from renewable sources that avoid competing with food production for land use. Ethylene glycol (EG), a C2 compound that can be recovered from plastic waste or derived from carbon dioxide, is gaining attention as a carbon source for microbial processes. Here, we systematically evaluate natural and synthetic metabolic pathways for EG assimilation using theoretical modeling approaches. We analyzed five pathways for their maximum theoretical yields, thermodynamic favourability, enzyme costs, and orthogonality to cell growth and identify favourable traits for each of these pathways for a given product. Our results reveal distinct trade-offs between pathway types. Synthetic pathways achieved higher theoretical yields for biomass and most bioproducts, with synthetic glycolaldehyde assimilation (SAGA) pathways showing the best overall yields and the synthetic acetyl-CoA assimilation (SACA) pathway demonstrating the highest thermodynamic favourability and lowest enzyme costs. Among natural pathways, the glycerate pathway exhibited favourable thermodynamics and moderate enzyme costs comparable to synthetic alternatives, while being particularly advantageous for glycolate production despite carbon losses. The β-hydroxyaspartate cycle (BHAC) showed the poorest thermodynamic performance and highest enzyme burden. However, natural pathways exhibited equal or higher orthogonality to growth-associated reactions, making them potentially suitable for dynamically controlled production systems. These findings provide guidance for selecting optimal EG assimilation strategies based on target products and process requirements, supporting the development of sustainable bioprocesses utilizing this promising unconventional feedstock. One-sentence summary This article reviews and compares metabolic pathways for the utilization of the compound EG in the context of bioproduction.</p>","PeriodicalId":16092,"journal":{"name":"Journal of Industrial Microbiology & Biotechnology","volume":" ","pages":""},"PeriodicalIF":4.5,"publicationDate":"2026-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12994477/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146202073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Finding of the positive impact of glucose on the production of indican over indigo in engineered Escherichia coli.","authors":"Hyun Jin Kim, Yeda Lee, Yuni Shin, Suhye Choi, Jinok Oh, Suwon Kim, Jungoh Ahn, Kwon-Young Choi, Jeong Chan Joo, Shashi Kant Bhatia, Yung-Hun Yang","doi":"10.1093/jimb/kuae048","DOIUrl":"10.1093/jimb/kuae048","url":null,"abstract":"<p><p>Indigo is a plant-based natural blue dye that can be produced via chemical synthesis and biological pathways. However, the toxic reduction processes and intracellular production of indigo through microbial metabolism are often limited by insolubility of indigo and complex downstream processing, causing environmental issues in the dyeing processes. Additionally, indican, a precursor of indigo with a glucose moiety, is highly soluble and can be easily converted into indoxyl by β-glucosidase, forming indigo under mild conditions. We constructed an indican-producing strain Escherichia coli BL21 HI201 by introducing a UDP-glycosyltransferase (ugt) into an indoxyl production system containing tryptophanse (tnaA) and flavin-containing monooxygenase (FMO) genes, enabling conversion of tryptophan into indican. Testing of the effect by various carbon sources suggested that glucose is one of the major factors affecting the ratio of indigo to indican, and increase in glucose concentration to more than 1.5% could produce sole indican without indigo. Under optimal conditions, E. coli BL21 HI201 biosynthesized 5.65 mM indican from tryptophan. Additionally, after deletion of various β-glucosidase genes, the bglA knockout strain E. coli BL21 HI204 produced more indican, achieving 6.79 mM after 24 hr of cultivation. This study demonstrated the strategic production of indican through the installation of a production system, deletion of a byproduct pathway, and control of glucose concentration.</p><p><strong>One-sentence summary: </strong>This paper demonstrates the strategic enhancement of indican production in genetically engineered Escherichia coli BL21 by optimizing metabolic pathways and controlling glucose concentrations.</p>","PeriodicalId":16092,"journal":{"name":"Journal of Industrial Microbiology & Biotechnology","volume":"52 ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11947661/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143730374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioprospecting and phylogenetic analysis of Priestia flexa AW3: an industrially significant amylase-producing bacterium from unexplored contaminated soil in Layyah.","authors":"Sobia Ramzan, Musrat Shaheen, Mohsin Khurshid, Farhat Jabeen, Sara Mahmood, Ayesha Sarwar, Shahzad Ahmad","doi":"10.1093/jimb/kuaf034","DOIUrl":"10.1093/jimb/kuaf034","url":null,"abstract":"<p><p>In spite of wonderful industrial applications of microbial enzymes, still most of habitats in various parts of world are unexplored for bioprospecting of industrial potent microbes. This study represents the first bioprospecting effort in Layyah district to explore indigenous bacterial diversity, enzymatic potential, and phylogenetic relationships in untapped contaminated soil habitat. Contaminated soils serve as reservoirs of industrially significant bacteria with unique enzymatic degradation capabilities, offering solutions for sustainable industrial applications and environmental remediation. An effort for comparative bioprospecting-based study for bacterial diversity exhibiting amylase potential across unaddressed contaminated soil samples [industrial, household, poultry, and animal waste (AW)] using qualitative and quantitative methods, was conducted. AW-contaminated soil exhibited the highest bacterial load (2.51 × 1010 CFUs) and amylase activity (51, amylase zones), whereas industrial waste soil showed the lowest CFUs (1.24 × 1010). Household waste soil, however, displayed the greatest Shannon diversity index (H'= 2.192262) for amylase-producing bacteria. Among isolates, Priestia flexa AW3 (OQ446563) demonstrated exceptional amylase production, forming 30 mm hydrolysis zones on starch agar and achieving optimal activity (1.76 ± 0.05 OD; 1.23 ± 0.03 AU/mL) at pH 7 and 37°C after 48 h. Notably, the strain retained enzymatic stability under extreme conditions, temperature up to 50°C, NaCl concentrations (0.5%-10%), and a broad pH range. Phylogenetic analysis via 16S rRNA sequencing confirmed its identity as P. flexa. This study underscores the untapped potential of contaminated soils in Layyah as sources of robust industrial microbes and highlights the value of bioprospecting in discovering novel bacterial strains for biotechnology and environmental sustainability. One-Sentence Summary: Bioprospecting for industrially important bacteria with unique enzymatic potential from untapped habitats is highly needed to solve sever environmental problems.</p>","PeriodicalId":16092,"journal":{"name":"Journal of Industrial Microbiology & Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12640123/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145481523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}