Novel Supplementation of Fe3O4-Doped Green Carbonized Nanoparticles on Hydrogenases Genes and Microbial Biodiversity for Enhancing Biohydrogen Yield in Dark Fermentation Microbial Electrohydrogenesis Cells.

IF 3.2 4区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Hikmatullah Ahmadi, Anam Jalil, Sohail Khan, Irfan Ali Phulpoto, Zhang Chengyu, Zhisheng Yu
{"title":"Novel Supplementation of Fe3O4-Doped Green Carbonized Nanoparticles on Hydrogenases Genes and Microbial Biodiversity for Enhancing Biohydrogen Yield in Dark Fermentation Microbial Electrohydrogenesis Cells.","authors":"Hikmatullah Ahmadi, Anam Jalil, Sohail Khan, Irfan Ali Phulpoto, Zhang Chengyu, Zhisheng Yu","doi":"10.1093/jimb/kuaf016","DOIUrl":null,"url":null,"abstract":"<p><p>Achieving high-purity biohydrogen (Bio-H₂) production necessitates the suppression of hydrogenotrophic methanogens, as their activity can impede hydrogen yield. Various inoculum pretreatments have been employed to suppress methane-producing Microorganisms; however, these methods can negatively impact the enzymatic activity of hydrogen-producing microorganisms, thereby reducing hydrogen production. To address this challenge, this research investigates a novel approach to enhance Bio-H₂ production by activating microbial enzymes using magnetite Fe₃O₄-doped carbonized nanoparticles (NPs) derived from vegetable leaves (VLCFe₃O₄-NPs) within a coupled dark fermentation-microbial Electrohydrogenesis system. Characterization results revealed that VLCFe₃O₄-NPs exhibited cubic and spherical morphologies, with a small diameter of 1±100 nm and a mean crystallite size of 38.1 nm, indicating high purity. Fermentation tests investigated the impact of different nanoparticle dosages on Bio-H₂ generation, hydrogenase gene expression (Fe-Fe and Ni-Fe), and microbial biodiversity. Bio-H₂ production significantly improved with 500 mg/L VLCFe₃O₄-NPs, yielding 1.2-fold more than the control group, while even a low dose of 25 mg/L resulted in a 0.22-fold increase. Relative gene expression analysis using qPCR and the 2-ΔΔCT method demonstrated a 30-fold increase in Cbei 1773 (Fe-Fe hydrogenase) and a 23-fold increase in hucL (Ni-Fe hydrogenase) gene expression, along with an increase in 16S rDNA. Additionally, the abundance of biohydrogen-producing bacteria, Clostridium_sensu_stricto_1 and Clostridium_sensu_stricto_11, increased by 14.3% and 11.1%, respectively, compared to 4.9% and 3.9% in the control group. This research indicates that VLCFe₃O₄-NPs offer an eco-friendly solution for boosting biohydrogen production within DF-MECs systems, thereby supporting sustainable bioenergy generation.</p>","PeriodicalId":16092,"journal":{"name":"Journal of Industrial Microbiology & Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.2000,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Industrial Microbiology & Biotechnology","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1093/jimb/kuaf016","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Achieving high-purity biohydrogen (Bio-H₂) production necessitates the suppression of hydrogenotrophic methanogens, as their activity can impede hydrogen yield. Various inoculum pretreatments have been employed to suppress methane-producing Microorganisms; however, these methods can negatively impact the enzymatic activity of hydrogen-producing microorganisms, thereby reducing hydrogen production. To address this challenge, this research investigates a novel approach to enhance Bio-H₂ production by activating microbial enzymes using magnetite Fe₃O₄-doped carbonized nanoparticles (NPs) derived from vegetable leaves (VLCFe₃O₄-NPs) within a coupled dark fermentation-microbial Electrohydrogenesis system. Characterization results revealed that VLCFe₃O₄-NPs exhibited cubic and spherical morphologies, with a small diameter of 1±100 nm and a mean crystallite size of 38.1 nm, indicating high purity. Fermentation tests investigated the impact of different nanoparticle dosages on Bio-H₂ generation, hydrogenase gene expression (Fe-Fe and Ni-Fe), and microbial biodiversity. Bio-H₂ production significantly improved with 500 mg/L VLCFe₃O₄-NPs, yielding 1.2-fold more than the control group, while even a low dose of 25 mg/L resulted in a 0.22-fold increase. Relative gene expression analysis using qPCR and the 2-ΔΔCT method demonstrated a 30-fold increase in Cbei 1773 (Fe-Fe hydrogenase) and a 23-fold increase in hucL (Ni-Fe hydrogenase) gene expression, along with an increase in 16S rDNA. Additionally, the abundance of biohydrogen-producing bacteria, Clostridium_sensu_stricto_1 and Clostridium_sensu_stricto_11, increased by 14.3% and 11.1%, respectively, compared to 4.9% and 3.9% in the control group. This research indicates that VLCFe₃O₄-NPs offer an eco-friendly solution for boosting biohydrogen production within DF-MECs systems, thereby supporting sustainable bioenergy generation.

新型fe3o4掺杂绿色碳化纳米颗粒对加氢酶基因和微生物多样性的影响,以提高暗发酵微生物电氢细胞的生物产氢率。
实现高纯度的生物氢(Bio-H₂)生产需要抑制产氢产甲烷菌,因为它们的活性会阻碍产氢。各种接种预处理已被用于抑制产甲烷微生物;然而,这些方法会对产氢微生物的酶活性产生负面影响,从而减少产氢。为了解决这一挑战,该研究研究了一种新的方法,通过在一个耦合的暗发酵-微生物电氢系统中使用来自蔬菜叶的Fe₃O₄掺杂碳化纳米颗粒(VLCFe₃O₄-NPs)激活微生物酶来提高Bio-H₂的产量。表征结果表明,所制得的VLCFe₃O₄-NPs具有立方和球形形貌,粒径小(1±100 nm),平均晶粒尺寸为38.1 nm,纯度高。发酵试验考察了不同纳米颗粒用量对Bio-H₂生成、加氢酶基因表达(Fe-Fe和Ni-Fe)以及微生物多样性的影响。500 mg/L的VLCFe₃O₄-NPs显著提高了Bio-H₂的产量,比对照组提高了1.2倍,即使是25 mg/L的低剂量也能提高0.22倍。qPCR和2-ΔΔCT方法的相对基因表达分析显示,Cbei 1773 (Fe-Fe氢化酶)和hul (Ni-Fe氢化酶)基因表达分别增加了30倍和23倍,16S rDNA也增加了。产氢菌Clostridium_sensu_stricto_1和Clostridium_sensu_stricto_11的丰度分别比对照组的4.9%和3.9%提高了14.3%和11.1%。该研究表明,VLCFe₃O₄-NPs为促进df - mes系统内的生物氢生产提供了一种环保解决方案,从而支持可持续的生物能源生产。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Journal of Industrial Microbiology & Biotechnology
Journal of Industrial Microbiology & Biotechnology 工程技术-生物工程与应用微生物
CiteScore
7.70
自引率
0.00%
发文量
25
审稿时长
3 months
期刊介绍: The Journal of Industrial Microbiology and Biotechnology is an international journal which publishes papers describing original research, short communications, and critical reviews in the fields of biotechnology, fermentation and cell culture, biocatalysis, environmental microbiology, natural products discovery and biosynthesis, marine natural products, metabolic engineering, genomics, bioinformatics, food microbiology, and other areas of applied microbiology
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信