从建筑用水性涂料中分离出抗生物杀灭剂的油酸假单胞菌。

IF 3.2 4区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Muatasem Latif Ali, Lionel Ferrieres, Tuulia Hyötyläinen, Jana Jass
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引用次数: 0

摘要

杀菌剂在工业应用中对减少微生物生长和防止产品变质至关重要。水性建筑涂料在制造和储存过程中容易受到微生物污染,这会对产品性能产生不利影响,缩短保质期并导致大量商业损失。降低水性涂料中杀菌剂浓度的未来趋势引起了人们对耐杀菌剂微生物出现的担忧。本研究旨在鉴定和表征从建筑水性涂料中分离的抗杀菌剂微生物,以更好地了解其耐药机制。从一个生产设施的变质产品、原材料和水中共收集了63个样品,在所有经过杀菌剂处理的样品中都鉴定出了橄榄油假单胞菌P4A。对P. oleovorans参考菌株1045与P4A分离株进行比较,发现其菌落形态、生长速度和对杀菌剂和抗生素的敏感性均有显著差异。P4A对5-氯-2-甲基-异噻唑啉-3-酮(CMIT)的抗性比对照菌株高3倍,对苯并噻唑啉酮(BIT)的抗性比对照菌株高1.5倍。相反,与参考菌株相比,它对甲基异噻唑啉酮(MIT)的敏感性高1.4倍。未见抗生素交叉耐药。液相色谱结合高分辨率质谱对脂质和极性代谢物进行代谢组学分析,发现P4A的脂质含量相对较高,而1045的极性代谢物含量相对较高。油脂组成,特别是二酰基甘油、磷脂酸、磷脂酰胆碱和磷脂酰丝氨酸在P. oleovorans菌株1045和P4A之间存在显著差异。这些差异突出了P. oleovorans P4A的脂质代谢增加,这可能有助于其对杀菌剂的适应。微生物耐药性可以直接影响这些产品的有效性,导致对频繁维护和更换的需求增加,安全问题和环境影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Biocide-resistant Pseudomonas oleovorans isolated from water-based coatings used in construction.

Biocides are crucial in industrial applications to minimize microbial growth and prevent product spoilage. Water-based construction coatings are susceptible to microbial contamination during manufacturing and storage and this adversely impacts product properties, reduces shelf-life and leads to substantial commercial losses. The future trend to lower the biocide concentrations in water-based coatings raises concerns about the emergence of biocide-resistant microbes. This study aims to identify and characterize the biocide-resistant microbe isolated from construction water-based coating materials to better understand its mechanisms of resistance. A total of 63 samples were collected from spoiled products, raw materials, and water from a manufacturing facility, and Pseudomonas oleovorans P4A were identified in all biocides-treated samples. A comparison between a P. oleovorans reference strain, 1045, and the P4A isolate revealed distinct colony morphology, growth rate and sensitivity to biocides and antibiotics. The P4A isolate was 3-fold more resistant to 5-chloro-2-methyl-isothiazolin-3-one (CMIT) and 1.5-fold more resistant to benzothiazolinone (BIT) compared to the reference strain. Conversely, it was 1.4-fold more sensitive to methylisothiazolinone (MIT) compared to the reference strain. No cross-resistance to antibiotics was observed. Metabolomic analysis using liquid chromatography combined with high-resolution mass spectrometry of lipids and polar metabolites showed that P4A had a relatively higher amount of lipids, while 1045 had a relatively higher amount of polar metabolites identified. A significant difference in lipid composition, specifically in diacylglycerol, phosphatidic acid, phosphatidylcholine, and phosphatidylserine was observed between P. oleovorans strains 1045 and P4A. These distinctions highlight increased lipid metabolism in P. oleovorans P4A and this may contribute to its adaptation to biocides. Microbial resistance can directly affect the effectiveness of these products, leading to an increased need for frequent maintenance and replacement, safety concerns, and environmental implications.

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来源期刊
Journal of Industrial Microbiology & Biotechnology
Journal of Industrial Microbiology & Biotechnology 工程技术-生物工程与应用微生物
CiteScore
7.70
自引率
0.00%
发文量
25
审稿时长
3 months
期刊介绍: The Journal of Industrial Microbiology and Biotechnology is an international journal which publishes papers describing original research, short communications, and critical reviews in the fields of biotechnology, fermentation and cell culture, biocatalysis, environmental microbiology, natural products discovery and biosynthesis, marine natural products, metabolic engineering, genomics, bioinformatics, food microbiology, and other areas of applied microbiology
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