Journal of Evolutionary Biochemistry and Physiology最新文献

{"title":"Maternal Methyl-Enriched Diet Improves Episodic Memory but Does Not Affect Conditioned Fear Memory in Offspring of WAG/Rij Rats","authors":"E. A. Fedosova, A. B. Shatskova, K. Yu. Sarkisova","doi":"10.1134/s0022093024030311","DOIUrl":"https://doi.org/10.1134/s0022093024030311","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>This study was aimed to find out what effect the maternal\u0000methyl-enriched diet (MED) in the perinatal period exerts on cognitive\u0000functions in adult offspring of WAG/Rij rats, a genetic model of\u0000absence epilepsy with comorbid depression. The cognitive functions\u0000(learning and memory) were evaluated in the novel object recognition\u0000(NOR) and fear conditioning (for fear memory) tests. The experiments\u0000were carried out on 6-month-old male WAG/Rij rats. The mothers of\u0000the experimental group fed a MED, while the mothers of the control\u0000group fed a control diet. The NOR test was used to assess episodic memory.\u0000This test is based on innate exploratory (novelty preference) motivation,\u0000which is usually reduced in depression. The conditioning of fear\u0000memory is the classical Pavlovian defensive conditioned reflex,\u0000manifested as freezing in response to negative reinforcement (electric\u0000footshock). In the NOR test, in rats of the experimental group,\u0000the novel object recognition index (a measure of episodic memory)\u0000and the number of entries to the center of the arena (a measure\u0000of exploratory motivation) were significantly higher compared to\u0000the corresponding measures in the control group. However, no intergroup\u0000differences were revealed in the manifestation of conditioned fear\u0000memory (% of freezing responses) to a context and a sound cue. The\u0000results indicate that the maternal MED improves episodic memory\u0000in the NOR test but does not affect the conditioned fear memory\u0000associated with a context and sound cue in the adult offspring of\u0000WAG/Rij rats.</p>","PeriodicalId":15805,"journal":{"name":"Journal of Evolutionary Biochemistry and Physiology","volume":"10871 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141522271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Suppression of Aseptic Inflammation Reduces the Severity of Pulmonary Artery Remodeling and Improves the Clinical Course of Experimental Chronic Thromboembolic Pulmonary Hypertension","authors":"A. A. Karpov, A. A. Krylov, L. A. Shilenko, A. M. Mihailova, D. D. Vaulina, D. Yu. Ivkin, N. P. Isakova, A. V. Vorotilov, N. Yu. Semenova, V. A. Zinserling, M. M. Galagudza","doi":"10.1134/s002209302403030x","DOIUrl":"https://doi.org/10.1134/s002209302403030x","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Chronic thromboembolic pulmonary hypertension (CTEPH) is a\u0000complication of pulmonary embolism, characterized by high blood\u0000pressure in the pulmonary artery combined with impaired lysis of fibrin\u0000clots. Previously, the presence of aseptic inflammation in CTEPH\u0000was found in the wall of the pulmonary artery branches, as well\u0000as perivascularly. However, the role of this inflammation in shaping CTEPH\u0000is unknown. The aim of the work was to study the effect of aseptic\u0000inflammation on CTEPH formation and progression. The experiments\u0000were carried out on male Wistar rats (<i>n</i> =\u000054). The CTEPH model was reproduced by repeated intravenous administration\u0000of partially biodegradable microspheres (MS). Immediately after\u0000the last MS administration, all animals were randomly allocated\u0000into four groups: (1) control (c.CTEPH) group—physiological saline\u0000was administered intramuscularly (i.m.) for 6 weeks; (2) low-dose\u0000prednisolone (LDP) group—prednisolone was administered i.m. at a\u0000dose of 1.5 mg/kg; (3) high-dose prednisolone (HDP) group—prednisolone\u0000was administered i.m. at a dose of 6 mg/kg; (4) healthy or intact\u0000(INT) group. After 6 weeks, there were performed the treadmill test, transthoracic\u0000echocardiography, cardiac catheterization with blood pressure manometry,\u0000and lung tissue histological examination. In a separate series of\u0000experiments, the degree of vascular wall and perivascular space\u0000inflammatory infiltration was assessed immunohistochemically. In\u0000LDP group, the vascular wall hypertrophy index (HI) and the percentage\u0000of collagen fibers in the vascular wall were reduced compared to\u0000the control group, with the HI being reduced significantly greater\u0000than in HDP group. In the latter, there was revealed a positive\u0000effect of high-dose prednisolone on the percentage of collagen fibers\u0000in the vascular wall, with this parameter being non-significantly\u0000different from that in intact animals. By immunohistochemical data,\u0000low-dose prednisolone effectively suppressed inflammatory infiltration\u0000of the vascular wall and perivascular space. Thus, we revealed the\u0000ability of low-dose prednisolone to reduce the degree of remodeling\u0000of the pulmonary artery branches by suppressing aseptic inflammation.</p>","PeriodicalId":15805,"journal":{"name":"Journal of Evolutionary Biochemistry and Physiology","volume":"25 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141522270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Synergistic Trio of Metabolic Master Regulators Sustaining a Vicious Circle of Pathological Processes in Post-Traumatic Stress Disorder","authors":"M. V. Kondashevskaya, K. A. Artemyeva, L. M. Mikhaleva","doi":"10.1134/s0022093024030116","DOIUrl":"https://doi.org/10.1134/s0022093024030116","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Post-traumatic stress disorder (PTSD) is a maladaptive response\u0000to the exposure of an extraordinary-intensity stressor. The animal\u0000and human body responds to any external challenges at the systemic, organismal\u0000and cellular levels. In stress-sensitive individuals, the collaboration\u0000of stress-implementing and stress-limiting systems is compromised,\u0000causing the transformation of behavior, cognitive abilities and\u0000other functions of the central nervous system. It is commonly accepted\u0000now that changes in the abundance and repertoire of the intestinal\u0000microbiota play an important role in the pathogenesis of PTSD. In\u0000this regard, the methods of improving the microflora are high on\u0000the agenda. Analyzing the literature data, we came to the conclusion\u0000that metabolic, somatic and mental health largely depends on the\u0000coordinated functioning of the key interdependent metabolic components:\u0000hepatobiliary system, intestinal microbiota and, as we believe,\u0000the state of mast cells. Close scrutiny of the interplay of these components\u0000will help identify new therapeutic targets and the most effective\u0000methods to treat PTSD.</p>","PeriodicalId":15805,"journal":{"name":"Journal of Evolutionary Biochemistry and Physiology","volume":"96 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141522290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Glibenclamide Prevents Inflammation by Targeting NLRP3 Inflammasome Activation in vitro","authors":"E. D. Khilazheva, Yu. A. Panina, A. I. Mosiagina, O. S. Belozor, Yu. K. Komleva","doi":"10.1134/s002209302403013x","DOIUrl":"https://doi.org/10.1134/s002209302403013x","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>The NLRP3 inflammasome plays a significant role in the development\u0000of neurodegeneration, physiological aging and metabolic inflammation,\u0000which actualizes the search for effective NLRP3 inflammasome inhibitors\u0000and assessing their effects. This study was aimed to assess the\u0000effect of pharmacological modulation of NLRP3 activity by an indirect\u0000NLRP3 inflammasome inhibitor, glibenclamide, on the in vitro expression\u0000of metaflammasome components in brain cells derived from middle-aged\u0000mice. It was found that glibenclamide reduces the expression of\u0000pro-inflammatory markers NLRP3 and IL18 in cell culture, which in\u0000turn prevents the phosphorylation of metaflammasome complex protein\u0000kinases, PKR and IKKβ. At the same time, there were no changes in\u0000the expression of pathologically phosphorylated IRS, as well as\u0000in the number of senescent cells in cultures after glibenclamide\u0000exposure.</p>","PeriodicalId":15805,"journal":{"name":"Journal of Evolutionary Biochemistry and Physiology","volume":"4 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141522292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spatial-Temporal Structure Features of Muscle Synergies during Profiling Elements Implementation in Rhythmic Gymnastics","authors":"S. A. Moiseev, S. M. Ivanov","doi":"10.1134/s0022093024030165","DOIUrl":"https://doi.org/10.1134/s0022093024030165","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>The article presents an analysis of the muscle synergies involved\u0000in making ball throws in different directions and from different\u0000starting positions. The study involved 6 highly qualified athletes\u0000engaged in rhythmic gymnastics. Electromyograms of the superficial\u0000muscles of the upper extremities and trunk were recorded synchronously\u0000during the movements, as well as video capture of the movements\u0000of body segments. Synergy parameters were extracted from the recorded\u0000signals using factor analysis using the principal component method.\u0000It has been established that individuals who have mastered a complex motor\u0000skill can demonstrate similar motor control strategies. This is\u0000manifested in the structuring of intermuscular interaction in the\u0000form of two general modules, the degree of involvement of muscles\u0000in which is highly similar in the implementation of different motor\u0000tasks. The first muscle module implements active movement of the\u0000upper limb by jointly activating the muscles of the forearm, shoulder and\u0000upper shoulder girdle, and also provides stabilization of the position\u0000of the trunk by synchronizing the activity of the muscles of the\u0000back and abdomen. The second muscle module is specific and is mainly associated\u0000with high involvement in the synergy of the muscles of the upper\u0000shoulder girdle and forearm for the implementation of exercises\u0000performed without visual control, or from starting positions that limit\u0000the mobility of certain parts of the body. Thus, the solution to\u0000the problem of coordination of many elements of the motor system\u0000may consist not only in reducing the number of executive organs—muscles involved\u0000in control, but also in organizing control variables in the form\u0000of modules at the kinematic level and their stabilization through\u0000muscle synergies.</p>","PeriodicalId":15805,"journal":{"name":"Journal of Evolutionary Biochemistry and Physiology","volume":"2012 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141522359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Importance of Adrenal Hormones in the Implementation of Stress-Protective Effect of Low L-Thyroxine Doses","authors":"E. A. Gusakova, I. V. Gorodetskaya","doi":"10.1134/s0022093024030141","DOIUrl":"https://doi.org/10.1134/s0022093024030141","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Although low L-thyroxine doses have previously been found\u0000to increase body’s stress resistance, it has remained unclear whether\u0000this effect is related to the action of L-thyroxine itself or is\u0000mediated by its activating/potentiating impact on other hormones.\u0000The aim of this work was to study the role of adrenal hormones in\u0000the implementation of the protective effect of low L-thyroxine doses\u0000under stress. The work was carried out on white mongrel male rats;\u0000stress was modeled by a time deficit situation. L-thyroxine was\u0000administered at low, close to physiological, doses according to\u0000the following schedule: week 1—1.5 µg/kg, week 2—2.0 µg/kg, week\u00003—2.5 µg/kg, week 4—3.0 µg/kg. It was found that stress exposure\u0000(1) increases the blood concentration of iodine-containing thyroid\u0000hormones (ITH) by 23–44%, corticosteroids by 40%, and adrenal relative\u0000mass (RM) by 31%, (2) reduces thymic and splenic RMs by 26 and 14%,\u0000respectively, and insulin level by 46%, (3) causes damage to the\u0000gastric mucosa (GM) with a damage index (DI) of 1.36, (4) increases\u0000animal physical endurance, locomotor activity and anxiety, while\u0000decreasing the intensity of exploratory responses. Bilateral adrenalectomy\u0000(BA) prevents the activation of thyroid function, abolishes the\u0000increase in physical endurance, and alters the structure of behavior,\u0000as well as determines a more severe damage to the GM (DI 2.7), an\u0000increase in anxiety, and the suppression of exploratory behavior\u0000under stress. BA limits the protective effect of L-thyroxine under stress,\u0000as manifested by lower values of rat horizontal locomotor and exploratory\u0000activity, higher anxiety, and damage to the GM (after L-thyroxine\u0000administration, DI under stress without BA is 0.12, against BA is\u00001.19). This corresponds to a lower blood ITH concentration (by 14–43%).\u0000Hence, low L-thyroxine doses have a stress-protective effect. BA\u0000limits but does not completely prevents its implementation, suggesting\u0000that adrenal hormones play an important, albeit not crucial, role\u0000in shaping the L-thyroxine anti-stress effect.</p>","PeriodicalId":15805,"journal":{"name":"Journal of Evolutionary Biochemistry and Physiology","volume":"6 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141522293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of Rodent Grooming and Its Microstructure in Modern Neurobiological Studies","authors":"K. V. Apukhtin, A. D. Shevlyakov, M. M. Kotova, S. V. Amikishiev, V. D. Riga, A. D. Volgin, A. V. Kalueff","doi":"10.1134/s0022093024030219","DOIUrl":"https://doi.org/10.1134/s0022093024030219","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Grooming is a complex innate animal behavior that can serve\u0000as a marker of physiological state of rodents under stress. Here,\u0000we analyze the impact of various experimental factors, including\u0000genetic, pharmacological and physiological, on self-grooming behavior\u0000of laboratory mice and rats. Analyses of grooming behavior assess\u0000not only the number and duration of its bouts but also its microstructure,\u0000i.e. frequency, sequence, localization, and consistency. Rodent\u0000grooming and its microstructure can serve as a sensitive marker\u0000for changes in the brain, its response to stress, and predisposition\u0000to pathological conditions simulating such human mental disorders\u0000as obsessive-compulsive disorder, autism and depression. Studying\u0000rodent self-grooming microstructure can provide valuable information\u0000about the mechanisms of brain pathogenesis, having multiple translational\u0000implications for neuroscience research.</p>","PeriodicalId":15805,"journal":{"name":"Journal of Evolutionary Biochemistry and Physiology","volume":"31 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141522364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Ovarian Gonadotropin Stimulation under Conditions of Chronic Psychosocial Stress on the Quality of Murine Oocytes","authors":"D. A. Lebedeva, T. N. Igonina, E. Yu. Brusentsev, N. A. Shavshaeva, S. Ya. Amstislavsky","doi":"10.1134/s0022093024030244","DOIUrl":"https://doi.org/10.1134/s0022093024030244","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Chronic psychosocial stress can negatively affect the female\u0000reproductive system. Meanwhile, the effect of ovarian stimulation\u0000with gonadotropins during stress on the quality of oocytes remains\u0000poorly studied. This work was aimed to investigate the effects of\u0000chronic psychosocial stress on the quality of murine cumulus-oocyte\u0000complexes under the natural estrus cycle, as well as during the\u0000ovarian stimulation with exogenous gonadotropins, which is an important\u0000part of modern assisted reproductive technologies. Here we demonstrate\u0000that psychosocial stress does not affect the number of ovulating oocytes\u0000but deteriorates their quality, i.e. reduces the percentage of mature\u0000oocytes. In addition, stressed mice exhibited an enhanced accumulation\u0000of reactive oxygen species (ROS) in oocytes, accompanied by an increased\u0000apoptosis level in cumulus cells. Hormonal stimulation of the ovaries\u0000with gonadotropins alleviates the negative changes associated with\u0000psychosocial stress, normalizing ROS overproduction in oocytes and\u0000reducing apoptosis levels in cumulus cells.</p>","PeriodicalId":15805,"journal":{"name":"Journal of Evolutionary Biochemistry and Physiology","volume":"25 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141522367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Central Responses to Peripheral Inflammation May Include Decreased Expression of Key Apoptotic Protease Caspase-3 in the Brainstem","authors":"A. V. Bannova, G. T. Shishkina, N. N. Dygalo","doi":"10.1134/s0022093024030037","DOIUrl":"https://doi.org/10.1134/s0022093024030037","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Microglia activation by proinflammatory stimuli, including\u0000lipopolysaccharide (LPS), is considered among the risk factors for\u0000neurodegeneration, although LPS treatment may also have a neuroprotective effect,\u0000necessitating further analysis of the relationship between microglial\u0000activation and cell death regulators. Here, we carried out a comparative\u0000analysis of the expression of Iba-1, a protein marker for microglia\u0000activation, and caspase-3, a key executor protease of apoptosis,\u0000in the brainstem and prefrontal cortex of Wistar rats, depending\u0000on LPS dose and schedule of its intraperitoneal administration.\u0000One day after a single LPS administration at a dose of 0.5 mg/kg,\u0000Iba-1 and caspase-3 expression levels were indistinguishable from\u0000control values in both brain structures. A fourfold LPS administration\u0000at the same dose over 7 days (once in 2 days) led to a significant\u0000increase in Iba-1 expression levels in the brainstem one day after\u0000the last injection, which was accompanied by a significant decrease\u0000in caspase-3 expression. In the brainstem, the same effects were\u0000observed 7 days after a single LPS administration at a higher dose of\u00005 mg/kg. In the frontal cortex, by contrast, no changes in caspase-3\u0000expression were found in a 7-day experiment, while Iba-1 expression\u0000rose only following a single LPS administration at 5 mg/kg. The detected\u0000decrease in caspase-3 expression in the brainstem under neuroinflammatory\u0000conditions may reflect the development of adaptive neuroprotective\u0000processes, particularly important for the brain structure responsible\u0000for such key body’s functions as respiration, blood pressure, and\u0000heartbeat.</p>","PeriodicalId":15805,"journal":{"name":"Journal of Evolutionary Biochemistry and Physiology","volume":"28 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141505973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Biopolymers and Functionalized by Them Vaterite Microparticles on Platelet Aggregation","authors":"D. V. Grigorieva, E. V. Mikhalchik, N. G. Balabushevich, D. V. Mosievich, M. A. Murina, O. M. Panasenko, A. V. Sokolov, I. V. Gorudko","doi":"10.1134/s0022093024030281","DOIUrl":"https://doi.org/10.1134/s0022093024030281","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Vaterite microparticles, metastable form of calcium carbonate,\u0000are promising forms of delivery of medicinal compounds. For more\u0000efficient delivery of target molecules (increased incorporation\u0000and retention), vaterite microparticles must be functionalized with\u0000biopolymers. In this article the effect of polysaccharides, mucin\u0000and vaterite microparticles, as well as hybrid vaterite microparticles\u0000with the above-mentioned biopolymers was studied on platelet aggregation.\u0000It was found that fucoidan, heparin and dextran sulfate (when added\u0000to platelet-rich plasma) and mucin (when added to isolated platelets) initiated\u0000cell aggregation. Pectin and chondroitin sulfate inhibited ADP-\u0000and thrombin-induced aggregation in a dose-dependent manner, mucin\u0000suppressed ADP-induced, and dextran sulfate suppressed thrombin-induced\u0000platelet aggregation. Vaterite microparticles at a concentration\u0000of 100–1000 µg/mL did not affect the aggregation of isolated platelets,\u0000but caused 10–15% cell aggregation in plasma; at the same time,\u0000at a concentration of 1000 µg/mL vaterite microparticles prevented\u0000agonist-induced cell aggregation by ~30%. It has been established\u0000that hybrid vaterite microparticles with fucoidan or heparin, when\u0000added both to platelet-rich plasma and to isolated cells, are capable\u0000to initiate platelet aggregation. Vaterite microparticles functionalized\u0000with pectin or chondroitin sulfate had no effect on spontaneous\u0000cell aggregation, and did not affect (with chondroitin sulfate)\u0000or inhibit (with pectin) agonist-induced platelet aggregation. Thus,\u0000the use of hybrid vaterite microparticles with pectin or fucoidan/heparin\u0000may be promising for the delivery of drugs aimed at modulating (inhibition\u0000with pectin or activation with fucoidan/heparin) the platelet component\u0000of hemostasis.</p>","PeriodicalId":15805,"journal":{"name":"Journal of Evolutionary Biochemistry and Physiology","volume":"21 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141522268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}