An Examination of the Role of Luteolin in Doxorubicin-Induced Testicular Damage

IF 0.6 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of Evolutionary Biochemistry and Physiology Pub Date : 2024-06-26 DOI:10.1134/s0022093024030086

N. Elagül-Tombul, İ. Söğüt, A. Köse-Vuruşkan

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Abstract

The purpose of this study was to assess the impact of luteolin (LUT) on testicular histology and biochemical changes in rats subjected to doxorubicin (DOX)-induced testicular damage. We used 34 male rats (8 weeks old) divided into four groups: Control group treated with normal saline on days 1, 7, 14, 21, and 28; LUT treated group (20 µg/kg daily for 28 days); DOX treated group (5 mg/kg on the same days as for the control group); DOX+LUT group (same scheme of treatment as for the LUT and DOX groups). Rats were sacrificed on day 29. Testicular tissue of the LUT group had normal histological structure. The DOX group had severe histopathological changes compared to the control which were significantly reversed by LUT treatment. Serum testosterone level in the DOX+LUT group was higher compared to the control and DOX groups while the malondialdehyde level decreased compared to the DOX group. Testicular malondialdehyde level of the DOX and DOX+LUT groups were higher than in the control and the total oxidant status of the DOX group was higher compared to the control while LUT treatment increased total antioxidant status in the DOX+LUT group. Luteolin had visible protective effect against testicular damage induced by doxorubicin, producing the improvement of histological structure by significantly enhancing antioxidant levels and reducing oxidant levels.

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研究叶黄素在多柔比星诱发的睾丸损伤中的作用

摘要本研究的目的是评估木犀草素（LUT）对多柔比星（DOX）所致大鼠睾丸损伤的睾丸组织学和生化变化的影响。我们将 34 只大鼠（8 周大）分为四组：对照组在第 1、7、14、21 和 28 天用生理盐水治疗；LUT 治疗组（每天 20 微克/千克，共 28 天）；DOX 治疗组（每天 5 毫克/千克，与对照组相同）；DOX+LUT 组（LUT 和 DOX 治疗方案相同）。LUT 组大鼠的睾丸组织结构正常，而 DOX 组大鼠的睾丸组织病理变化较对照组严重，LUT 治疗后可显著逆转。DOX+LUT组的血清睾酮水平高于对照组和DOX组，而丙二醛水平则低于DOX组。DOX组和DOX+LUT组的睾丸丙二醛水平高于对照组，DOX组的总抗氧化剂水平高于对照组，而LUT治疗则提高了DOX+LUT组的总抗氧化剂水平。木犀草素对多柔比星诱导的睾丸损伤具有明显的保护作用，通过显著提高抗氧化剂水平和降低氧化剂水平改善了组织学结构。

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来源期刊

Journal of Evolutionary Biochemistry and Physiology 生物-进化生物学

自引率

33.30%

发文量

110

审稿时长

6-12 weeks

期刊介绍： Journal of Evolutionary Biochemistry and Physiology publishes original experimental and theoretical and review articles related to evolution of the main forms of metabolism in connection with life origin; comparative and ontogenetic physiology and biochemistry, biochemical evolution of animal world; as well as evolution of functions; morphology, pharmacology, pathophysiology and ecological physiology. The journal welcomes manuscripts from all countries in the English or Russian language.