Journal of Food Biochemistry最新文献

{"title":"Network Pharmacology Prediction and Experimental Validation of Ferulic Acid’s Protective Effects against Diclofenac-Induced Liver Injury","authors":"Fatema S. Alatawi,&nbsp;Awatif M. E. Omran,&nbsp;Mohsen S. Alatawi,&nbsp;Eman Rashad,&nbsp;Noha A. E. Yasin,&nbsp;Ahmed F. Soliman","doi":"10.1155/2024/5592390","DOIUrl":"https://doi.org/10.1155/2024/5592390","url":null,"abstract":"<div>\u0000 <p>Despite being one of the most consumed analgesics worldwide, liver injury is an adverse effect of diclofenac (DF). In pursuit of reliable hepatoprotective natural remedies, this study aimed to investigate the potential protective effect of ferulic acid (FA) and its mechanism against DF-induced liver injury. Various network databases and datasets were used to collect targets corresponding to FA and DF-induced liver injury. Enrichment analyses of common targets were performed, a protein-protein interaction (PPI) network was constructed, the hub genes were identified, and the upstream miRNA interacting with the top hub gene was later predicted. A DF-induced liver injury rat model was established to verify FA’s protective effects, and the selected hub gene expression level with its upstream regulatory miRNA and a downstream set of targets was examined to elucidate the underlying mechanism. A total of 18 genes were identified as potential targets of FA to protect against DF-induced liver injury. Data from the enrichment and PPI analyses and the prediction of the upstream miRNAs indicated that the most worthwhile pair to study was miR-296-5p/<i>Jun</i>. <i>In vivo</i> findings showed that coadministration of FA significantly reduced the DF-induced alterations in the liver function indices, oxidative stress, and liver histology. Mechanistically, FA downregulated the expression of <i>Jun</i>, <i>Bim</i>, <i>Bax</i>, <i>Casp3</i>, IL-1<i>β</i>, IL-6, and TNF-<i>α</i>, whereas it upregulated the expression of rno-miR-296-5p and <i>Bcl2</i>. In conclusion, combining network pharmacology and an <i>in vivo</i> study revealed that miR-296-5p/<i>Jun</i> axis could mediate the mitigative effect of FA against DF-induced liver injury.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2024 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2024/5592390","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141631160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Abelmoschus esculentus Improves Hippocampal Function Associated with Dipeptidyl Peptidase-4 in High Fat Diet-Fed db/db Mice","authors":"Chiung-Huei Peng,&nbsp;Chau-Jong Wang,&nbsp;Yen-Yu Yang,&nbsp;Chien-Ning Huang,&nbsp;Huei-Jane Lee","doi":"10.1155/2024/5554538","DOIUrl":"https://doi.org/10.1155/2024/5554538","url":null,"abstract":"<div>\u0000 <p>Hippocampal function can be impaired by diabetes mellitus (DM) and obesity. <i>Abelmoschus esculentus</i> (AE) fractions reportedly mitigate the symptoms of Alzheimer’s disease (AD) by downregulating dipeptidyl peptidase-4 (DPP-4)-mediated insulin resistance. AE extracted by alcohol (fraction 1, F1) and water (fraction 2, F2) contained quercetin glycosides and polysaccharides, respectively. In this study, we investigated whether AE affects hippocampal function in <i>in vitro</i> and <i>in vivo</i> systems. Our results indicate that F1 or F2 enhanced neurogenesis and synapse formation in palmitate-treated hippocampal neural cells, presumably by downregulating DPP-4. In db/db mice fed with high fat diet, the hippocampal insulin resistance correlated spatial recognition, with fraction F2 improving hippocampal function. Of note, the alteration of neurogenesis seems interconnecting with changes in gut microbiota. In summary, AE can improve hippocampal function, attenuate insulin resistance, and promote neurogenesis by regulating DPP-4. AE, particularly F2, has the potential to serve as an adjuvant in preventing DM-associated AD.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2024 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2024/5554538","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141631213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antiviral Activity of Curcuminoids Derived from Deverra tortuosa Plant","authors":"Eman Elsharkawy,&nbsp;May Alqurashi,&nbsp;Mona Alshathly,&nbsp;A. K. M. Moyeenul Huq","doi":"10.1155/2024/5548871","DOIUrl":"https://doi.org/10.1155/2024/5548871","url":null,"abstract":"<div>\u0000 <p>Curcuminoids are natural products with widespread biological activity mostly extracted from <i>Curcuma longa</i> and its family. In the current study, we report the first time to isolate curcuminoids from another phyto source. Three compounds (curcumin, desmethoxycurcumin (DMC), and bisdemethoxycurcumin (BDMC)) were isolated from the plant Deverra tortuosa using methanolic extract, which was then fractionated and separated by puriFlash. The purity of compounds was monitored by thin-layer chromatography and confirmed by high-performance liquid chromatography. The mass spectrometry identified the isolated compound by their electrospray ionisation. Molecular docking then demonstrated that curcumin and BDMC bind at the same cavity (187 Å) on SARS-CoV-2 M^pro protein indicating similarities while DMC binds to a cavity with a larger size (372 Å). Among the three curcuminoids, BDMC was shown to give the highest binding energy (−7.5 kcal/mol) followed by curcumin (−7.4 kcal/mol) and DMC (−7.3 kcal/mol). Calculating the half-maximal cytotoxic concentration and inhibitory median concentration of both DMC and BDMC showed low-to-moderate antiviral activity against SARS-CoV-2.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2024 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2024/5548871","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141608008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Edible Bird’s Nest on Protecting against Cognitive Deficit and Ameliorating Beta-Amyloid in Hippocampal Rats’ Model of Cerebral Ischemia","authors":"Nualpun Sirinupong,&nbsp;Worapanit Chansuwan,&nbsp;Pratchaya Kaewkaen","doi":"10.1155/2024/7367894","DOIUrl":"https://doi.org/10.1155/2024/7367894","url":null,"abstract":"<div>\u0000 <p>Cerebral ischemia has been identified as the primary cause of global mortality, but there is currently no effective therapy for treating this condition. Consequently, the search for novel neuroprotective agents that can guard against stroke remains essential. Recent research has demonstrated the importance of alternative treatments in the pathology of cerebral ischemia leading to vascular dementia. The purpose of this study was to determine the effect of neurocognitive behavior and beta-amyloid markers in terms of the neuroprotective effects of Edible Bird’s Nest (EBN). Male adult Wistar rats were orally administered EBN at concentrations of 10, 50, and 100 mg/kg for 2 weeks prior to and 3 weeks after middle cerebral artery occlusion surgery to mimic vascular dementia. The rodents were then classified based on their neurological score, beta-amyloid accumulation, volume of cerebral infarction, and acetylcholinesterase activity. We assessed the animals’ spatial memory and administered the Morris water maze and radial arm maze tests. We found that EBN substantially decreased beta-amyloid in the hippocampus, decreased acetylcholinesterase activity, and enhanced the animals’ neurological scores and spatial memory. We conclude that EBN contains potential substances that promote learning and memory pathways. However, additional research is still necessary to confirm these findings.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2024 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2024/7367894","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141565814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Glucosamine Hydrochloride and Glucosamine-Gallic Acid Nanoparticles for the Treatment of Osteoarthritis: Synthesis, Antioxidant, and Anti-Inflammatory","authors":"Alika Jafari,&nbsp;Mehdi Tabarsa,&nbsp;Hossein Naderi-Manesh,&nbsp;Hassan Ahmadi Gavlighi,&nbsp;SangGuan You,&nbsp;Zahra Vaezi","doi":"10.1155/2024/3272099","DOIUrl":"https://doi.org/10.1155/2024/3272099","url":null,"abstract":"<div>\u0000 <p>This study aimed to evaluate the antioxidant and anti-inflammatory effects of glucosamine nanoparticles (GNPs) grafted with gallic acid (GNPs-<i>g</i>-GA). Glucosamine hydrochloride (G-HCl) was produced from shrimp shell, and then GNPs synthesized using ionic gelation method. GNPs-<i>g</i>-GA was prepared by coupling GNPs with GA via 1-ethy-3-(3-dimethylaminopropyl)-carbodiimide (EDC) in combination with N-hydroxysuccinimide (NHS) cross-linking agents. The results indicated that the grafting of GA onto GNPs at different ratios increased the average size of the nanoparticles from 195.7 to 294.2 nm with various grafting degrees ranging from 73.3 to 146.4 mg GA/g GNPs-<i>g</i>-GA. The SEM images revealed the formation of spherical-shaped GNPs-<i>g</i>-GA nanoparticles with approximate sizes ranging from 275.3 to 303.6 nm. The appearance of characteristic signals in the FT-IR (C=C, C–O/C–C, and NH₂) and ¹H-NMR (H-2 and H-6 at 6.95 ppm) spectra and the red shift in UV-Vis spectrum provided further support of GNPs-<i>g</i>-GA successful synthesis. DPPH radical scavenging (from 20.0 to 70.4%) and ABTS radical scavenging (from 18.7 to 79.0%) activities and reducing power (nearly fivefold) sharply improved in GNPs-<i>g</i>-GA. Moreover, GNPs-<i>g</i>-GA was found nontoxic and drastically reduced the level of nitric oxide release and downregulated the synthesis of TNF-<i>α</i>, IL-1<i>β</i>, and IL-6 in LPS-induced RAW2647 murine macrophage cells through NF-<i>κ</i>B and MAPKs signaling pathways. Overall, these results suggested that the grafting of GNPs and GA is an effective strategy for the suppression of inflammation response and oxidation reaction in osteoarthritis.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2024 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2024/3272099","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141487790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effects of Chia Supplementation on Lipid Profile in Patients Suffering from Metabolic Disorders: A Systematic Review and Meta-Analysis","authors":"Qi Run,&nbsp;Haoming Yan,&nbsp;Pedram Pam,&nbsp;Parsa Jamilian,&nbsp;Meysam Zarezadeh,&nbsp;Hongyan Zhang","doi":"10.1155/2024/5587140","DOIUrl":"https://doi.org/10.1155/2024/5587140","url":null,"abstract":"<div>\u0000 <p><i>Background</i>. Dyslipidemia is a major public health challenge worldwide, and chia seed, a nutrient-dense functional food, could be beneficial in metabolic disorders. This systematic review and meta-analysis were conducted to assess how chia seed intake affects lipid profiles. <i>Methods</i>. A comprehensive literature search was conducted on electronic databases including PubMed, Scopus, Web of Science, Cochrane, and Google Scholar up to January 2024. All randomized controlled trials (RCTs) addressing the effect of chia seed on triglyceride (TG), total cholesterol (TC), high-density lipoprotein (HDL), and low-density lipoprotein (LDL) were included. Data were analyzed using a random-effects model and reported as weighted mean differences (WMD) with 95% confidence intervals (CI). Subgroup and sensitivity analyses were also performed. The quality of studies was evaluated using the Cochrane risk of bias tool. <i>Results</i>. A total of seven RCTs involving 304 participants were included in the meta-analysis. The results showed that chia consumption had no considerable effect on TG (WMD: −18.73 mg/dl; 95% CI: −55.46 to 18.00; <i>p</i> = 0.31), TC (WMD: −7.49 mg/dl; 95% CI: −15.60 to 0.63; <i>p</i> = 0.07), HDL (WMD: −2.85 mg/dl; 95% CI: −6.74 to 1.05; <i>p</i> = 0.15), and LDL (WMD: −8.09 mg/dl; 95% CI: −18.17 to 1.99; <i>p</i> = 0.11). However, subgroup analyses indicated that chia could decrease TC in patients with obesity and type 2 diabetes mellitus (T2DM) and LDL in patients with obesity. <i>Conclusion</i>. Chia consumption had no effect on lipid profiles including triglyceride, total cholesterol, LDL, and HDL. Nevertheless, patients with obesity and T2DM might benefit from its supplementation.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2024 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2024/5587140","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141488347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Screening of Functional Lactic Acid Bacteria with Potential Production of Antimicrobial Peptides from Milk Proteins for Application as Preservative Dairy Starter","authors":"Khaled Elsaadany,&nbsp;Ehab Kheadr,&nbsp;Sarah Elmesseiry,&nbsp;Saeed Hamdy,&nbsp;Nassra Dabour","doi":"10.1155/2024/7634462","DOIUrl":"https://doi.org/10.1155/2024/7634462","url":null,"abstract":"<div>\u0000 <p>This study aimed to assess the molecular screening and safety of 14 lactic acid bacteria isolates for their capacity to produce antimicrobial peptides from milk proteins. The isolates were molecularly identified as <i>Enterococcus faecium</i>, <i>Enterococcus durans</i>, <i>Lactococcus lactis</i> subsp. <i>lactis</i>, <i>Lactococcus lactis</i> subsp. <i>hordniae</i>, <i>Lactiplantibacillus plantarum</i>, and <i>Weissella confusa</i>. The tested lactococci isolates produced bioactive peptides with a wild-type antimicrobial spectrum against <i>Salmonella typhimurium</i>, <i>Pseudomonas aeruginosa</i>, <i>Bacillus cereus</i>, and <i>Listeria monocytogenes</i>. The majority of isolates were susceptible to chloramphenicol, tetracycline, and ampicillin but resistant to nalidixic acid. <i>Lactococcus lactis</i> FFNL-2034 and <i>Weissella confusa</i> FFNL-1850 presented antibiotic multiresistance. Antibiotic resistance genes <i>bla</i>, <i>ermB</i>, <i>tetK</i>, <i>cat</i>, <i>vanA</i>, and <i>vanC</i> were absent in most of the tested isolates. The <i>ermC</i>, <i>strA</i>, <i>strB,</i> and <i>tetM</i> genes were positive in some isolates. These results provide safe LAB, which could act as a potent biopreservative agent for functional dairy products.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2024 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2024/7634462","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141488557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Production of Smart Packaging Chitosan Films Utilizing Malva sylvestris Mucilage and Rubia tinctorum Extract for Enhancing Fish Freshness and Prolonging Shelf Life","authors":"Meysam Soleymani Najafabadi,&nbsp;Amir Shakerian,&nbsp;Zohreh Mashak,&nbsp;Ebrahim Rahimi","doi":"10.1155/2024/7275427","DOIUrl":"https://doi.org/10.1155/2024/7275427","url":null,"abstract":"<div>\u0000 <p>In the dynamic landscape of food technology, where ensuring freshness and extending shelf life are paramount concerns, active packaging stands at the forefront of innovation. This study explores the utilization of natural resources in the development of smart packaging solutions aimed at enhancing the freshness of perishable foods. Specifically, chitosan films incorporating <i>Malva sylvestris</i> mucilage and <i>Rubia tinctorum</i> extract are investigated for their efficacy in preserving the quality of fish products. Through a series of experiments and analyses, this research elucidates the potential of these bio-based materials to revolutionize packaging practices, offering a sustainable and effective means of prolonging the shelf life of perishable goods while maintaining their quality and safety. Assessment of the antimicrobial properties of <i>Rubia tinctorum</i> hydroethanolic extract revealed that <i>Staphylococcus aureus</i> displayed higher susceptibility to this extract with the lowest MIC value (2.60 ± 0.09 mg/ml) and MBC value (5.20 ± 0.09 mg/ml) as well as the highest inhibition zone (16.33 ± 0.50 mm). For the composites with different concentrations of hydroethanolic <i>Rubia tinctorum</i> extracts, the lightness value significantly (<i>p</i> &lt; 0.05) increased at neutral pH. After days of storage, the pH in the fish sample surpassed the threshold (7), leading to a corresponding change in the indicator film’s color. Also, samples coated with higher levels of <i>Rubia tinctorum</i> extract in active/smart chitosan films with Malva sylvestris mucilage had lower pH, thiobarbituric acid, total volatile nitrogen, and microbial population (mesophile, psychrophile, and <i>Enterobacteriaceae</i>). The highest appearance score, surface glaze score, and overall odor acceptance in samples 4 (fish coated with active/smart chitosan with <i>Malva sylvestris</i> containing 0.6% <i>Rubia tinctorum</i> extract) and 5 (fish coated with active/smart chitosan with <i>Malva sylvestris</i> contained 1.2% <i>Rubia tinctorum</i> extract) (<i>p</i> ≤ 0.05) suggest that this application may contribute to the observed perceptual qualities. This novel indicator film has the potential to function as intelligent packaging for monitoring fish freshness. Additional research can delve into the precise mechanisms driving these effects and fine-tune the formulation for particular applications.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2024 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2024/7275427","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141488382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preharvest Salicylic Acid and Oxalic Acid Decrease Bioactive and Quality Loss in Blackberry (cv. Chester) Fruits during Cold Storage","authors":"Derya Erbas,&nbsp;Kerem Mertoglu,&nbsp;Ilknur Eskimez,&nbsp;Mehmet Polat,&nbsp;Mehmet A. Koyuncu,&nbsp;Melekber S. Durul,&nbsp;Ibrahim Bulduk,&nbsp;Barış Kaki,&nbsp;Tuba Esatbeyoglu","doi":"10.1155/2024/4286507","DOIUrl":"https://doi.org/10.1155/2024/4286507","url":null,"abstract":"<div>\u0000 <p>In this study, we investigated the effects of preharvest oxalic acid (2.5 mM (OA1) and 5 mM (OA2)) and salicylic acid (0.5 mM (SA1) and 1 mM (SA2)) treatments on the storage and quality of blackberry cv. Chester. We applied salicylic acid and oxalic acid to the plants seven and fourteen days before harvest and recorded the physiological, physicochemical, and external appearance characteristics of the fruits during the cold storage period (nine days). The results showed that the treatments decreased ethylene production by 29.5% (SA1) at harvest and by 28.5% (SA2) at the end of the storage period. In addition, the respiration rate was reduced between 13.0% (OA2) and 28.0% (SA2) compared to the control. Although fruit weigh loss increased as storage extended, the highest losses were observed in control with 1.35%. Titratable acidity decreased from harvest (0.46%) to the end of storage (0.39%) in the control, whereas it ranged from 0.52% (SA1) to 0.62% (OA1) in the treatment groups. Malic acid and syringic acid were the dominant organic acid and phenolic, respectively, and although their content decreased continuously in the control group, no loss was observed in the treated groups after the sixth day. These effects were positively reflected in the external appearance of the fruits, total phenolic content, total flavonoid content, and properties related to antioxidant activity. As a result of the cumulative evaluation of all parameters, it can be suggested that Chester fruits can be stored for nine days in marketable quality with the SA2 applications. Among oxalic acid concentrations, 2.5 mM can be recommended for phytochemical accumulation at harvest and short-term storage of three days.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2024 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2024/4286507","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141488221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary Supplementation with Omega-3 PUFAs Modulates Gut Microbiota and Protects the Intestinal Barrier in Senescence-Accelerated Mice","authors":"Jun Qiu,&nbsp;Shuo Qi,&nbsp;Chengming Ding,&nbsp;Haiping Jiang,&nbsp;Guodong Chen,&nbsp;Xin Lv","doi":"10.1155/2024/8925691","DOIUrl":"https://doi.org/10.1155/2024/8925691","url":null,"abstract":"<div>\u0000 <p>Intestinal mucosal barrier aging is a major cause of the occurrence and development of many chronic diseases in older adults and is closely associated with gut microbiota. However, it remains unclear whether exogenous supplementation with omega-3 polyunsaturated fatty acids (<i>ω</i>-3 PUFAs) can ameliorate aging-induced intestinal mucosal barrier damage by regulating the gut microbiota. This study was conducted to explore the roles of <i>ω</i>-3 PUFAs and gut microbiota in the process of maintaining the intestinal mucosal barrier. Senescence-accelerated mouse prone 8 (SAMP8) mice were used to establish a geriatric animal model and given reasonable doses of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) supplemented diets for six months to validate the effect of <i>ω</i>-3 PUFAs. Differences in the composition and function of gut microbiota were analyzed using 16S rRNA gene sequencing. This study revealed that aging SAMP8 mice showed increased intestinal permeability and gut microbiota disorder. Long-term supplementation with <i>ω</i>-3 PUFAs reduced intestinal mucosal permeability (<i>P</i> &lt; 0.05), regulated gut microbiota, strengthened the microecological interactions, and enhanced enrichment of metabolic pathways producing short-chain fatty acids (<i>P</i> &lt; 0.05), ultimately ameliorating intestinal mucosal barrier dysfunction. Furthermore, we demonstrated that <i>ω</i>-3 PUFAs could maintain the aging intestinal mucosal barrier by regulating the relative abundance of eight key characteristic microbial strains to increase the concentration of DHA and EPA in the plasma and colon tissue and reduced intestinal permeability of SAMP8 mice. <i>ω</i>-3 PUFAs can significantly ameliorate intestinal mucosal barrier function in senescence-accelerated mice, and this effect is mediated by the gut microbiota. The findings of our study provide a scientific basis for the rational intake of <i>ω</i>-3 PUFAs in older adults to maintain intestinal mucosal barrier function.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2024 1","pages":""},"PeriodicalIF":4.0,"publicationDate":"2024-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2024/8925691","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141424800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}