{"title":"副干酪乳杆菌N1115对2型糖尿病大鼠血糖、炎症、认知功能和肠道菌群组成的改善作用","authors":"Qing Li, Feng Feng, Yanqing Bian, Hanyi Zhao, Sijia Cao, Dongzhe Sun, Tianyue Jiao, Baohua Zhao, Liping Peng","doi":"10.1155/jfbc/6891209","DOIUrl":null,"url":null,"abstract":"<div>\n <p>This study aims to investigate the effect of probiotic <i>Lactobacillus paracasei</i> N1115 on Type 2 diabetes mellitus (T2DM) rats. SD rats were randomly divided into 5 groups, including control group (ND), diabetes mellitus (DM) group, and high (10<sup>9</sup> CFU/mL), medium (10<sup>8</sup> CFU/mL), or low (10<sup>7</sup> CFU/mL) doses of probiotics intervention groups. The ND rats were fed a normal diet, and the others were fed a high-fat diet and injected with streptozotocin (STZ) to build a T2DM model. In the three probiotic intervention groups, rats were given high, medium, or low doses of <i>L. paracasei</i> N1115 by gavage for 12 weeks. Results indicated that probiotics could prevent weight loss and decrease the blood glucose levels of T2DM rats, in a dose-dependent manner. A high dose of <i>L. paracasei</i> N1115 also led to a significant decline in the inflammatory response by regulating the TLR4/NF-κB inflammatory pathway and reducing proinflammatory cytokines, such as tumor necrosis factor-<i>α</i> (TNF-α), interleukin-1β (IL-1β), and lipopolysaccharide (LPS) (<i>p</i> < 0.05). Besides, the memory factor cAMP and the cognitive regulatory pathway BDNF/TrkB were increased by high dose of <i>L. paracasei</i> N1115 treatment (<i>p</i> < 0.05), revealing an enhanced cognitive memory competence. In addition, analysis of the gut microbiome showed that a high dose of <i>L.paracasei</i> N1115 decreased Firmicutes/Bacteroidota ratio and increased the relative abundance of beneficial bacteria, such as unclassified_Lachnospiraceae, Ligilactobacillus, and Lachnospiraceae_NK4A136_group. The microbial gene contents associated with metabolic pathways, nicotinate and nicotinamide metabolism, glycine, serine, and threonine metabolism and the citrate cycle were upregulated under <i>L. paracasei</i> N1115 treatment. In summary, this study elucidated the regulatory effects of <i>L. paracasei</i> N1115 on T2DM in rats and revealed a promising dietary supplement for the treatment of T2DM.</p>\n </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2025 1","pages":""},"PeriodicalIF":3.5000,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/6891209","citationCount":"0","resultStr":"{\"title\":\"Amelioratory Role of Lactobacillus paracasei N1115 on Blood Glucose, Inflammation, Cognitive Function, and Gut Microbiota Composition in Type 2 Diabetes Mellitus Rats\",\"authors\":\"Qing Li, Feng Feng, Yanqing Bian, Hanyi Zhao, Sijia Cao, Dongzhe Sun, Tianyue Jiao, Baohua Zhao, Liping Peng\",\"doi\":\"10.1155/jfbc/6891209\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n <p>This study aims to investigate the effect of probiotic <i>Lactobacillus paracasei</i> N1115 on Type 2 diabetes mellitus (T2DM) rats. SD rats were randomly divided into 5 groups, including control group (ND), diabetes mellitus (DM) group, and high (10<sup>9</sup> CFU/mL), medium (10<sup>8</sup> CFU/mL), or low (10<sup>7</sup> CFU/mL) doses of probiotics intervention groups. The ND rats were fed a normal diet, and the others were fed a high-fat diet and injected with streptozotocin (STZ) to build a T2DM model. In the three probiotic intervention groups, rats were given high, medium, or low doses of <i>L. paracasei</i> N1115 by gavage for 12 weeks. Results indicated that probiotics could prevent weight loss and decrease the blood glucose levels of T2DM rats, in a dose-dependent manner. A high dose of <i>L. paracasei</i> N1115 also led to a significant decline in the inflammatory response by regulating the TLR4/NF-κB inflammatory pathway and reducing proinflammatory cytokines, such as tumor necrosis factor-<i>α</i> (TNF-α), interleukin-1β (IL-1β), and lipopolysaccharide (LPS) (<i>p</i> < 0.05). Besides, the memory factor cAMP and the cognitive regulatory pathway BDNF/TrkB were increased by high dose of <i>L. paracasei</i> N1115 treatment (<i>p</i> < 0.05), revealing an enhanced cognitive memory competence. In addition, analysis of the gut microbiome showed that a high dose of <i>L.paracasei</i> N1115 decreased Firmicutes/Bacteroidota ratio and increased the relative abundance of beneficial bacteria, such as unclassified_Lachnospiraceae, Ligilactobacillus, and Lachnospiraceae_NK4A136_group. The microbial gene contents associated with metabolic pathways, nicotinate and nicotinamide metabolism, glycine, serine, and threonine metabolism and the citrate cycle were upregulated under <i>L. paracasei</i> N1115 treatment. In summary, this study elucidated the regulatory effects of <i>L. paracasei</i> N1115 on T2DM in rats and revealed a promising dietary supplement for the treatment of T2DM.</p>\\n </div>\",\"PeriodicalId\":15802,\"journal\":{\"name\":\"Journal of Food Biochemistry\",\"volume\":\"2025 1\",\"pages\":\"\"},\"PeriodicalIF\":3.5000,\"publicationDate\":\"2025-02-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/6891209\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Food Biochemistry\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1155/jfbc/6891209\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Food Biochemistry","FirstCategoryId":"97","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1155/jfbc/6891209","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
Amelioratory Role of Lactobacillus paracasei N1115 on Blood Glucose, Inflammation, Cognitive Function, and Gut Microbiota Composition in Type 2 Diabetes Mellitus Rats
This study aims to investigate the effect of probiotic Lactobacillus paracasei N1115 on Type 2 diabetes mellitus (T2DM) rats. SD rats were randomly divided into 5 groups, including control group (ND), diabetes mellitus (DM) group, and high (109 CFU/mL), medium (108 CFU/mL), or low (107 CFU/mL) doses of probiotics intervention groups. The ND rats were fed a normal diet, and the others were fed a high-fat diet and injected with streptozotocin (STZ) to build a T2DM model. In the three probiotic intervention groups, rats were given high, medium, or low doses of L. paracasei N1115 by gavage for 12 weeks. Results indicated that probiotics could prevent weight loss and decrease the blood glucose levels of T2DM rats, in a dose-dependent manner. A high dose of L. paracasei N1115 also led to a significant decline in the inflammatory response by regulating the TLR4/NF-κB inflammatory pathway and reducing proinflammatory cytokines, such as tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and lipopolysaccharide (LPS) (p < 0.05). Besides, the memory factor cAMP and the cognitive regulatory pathway BDNF/TrkB were increased by high dose of L. paracasei N1115 treatment (p < 0.05), revealing an enhanced cognitive memory competence. In addition, analysis of the gut microbiome showed that a high dose of L.paracasei N1115 decreased Firmicutes/Bacteroidota ratio and increased the relative abundance of beneficial bacteria, such as unclassified_Lachnospiraceae, Ligilactobacillus, and Lachnospiraceae_NK4A136_group. The microbial gene contents associated with metabolic pathways, nicotinate and nicotinamide metabolism, glycine, serine, and threonine metabolism and the citrate cycle were upregulated under L. paracasei N1115 treatment. In summary, this study elucidated the regulatory effects of L. paracasei N1115 on T2DM in rats and revealed a promising dietary supplement for the treatment of T2DM.
期刊介绍：
The Journal of Food Biochemistry publishes fully peer-reviewed original research and review papers on the effects of handling, storage, and processing on the biochemical aspects of food tissues, systems, and bioactive compounds in the diet.
Researchers in food science, food technology, biochemistry, and nutrition, particularly based in academia and industry, will find much of great use and interest in the journal. Coverage includes:
-Biochemistry of postharvest/postmortem and processing problems
-Enzyme chemistry and technology
-Membrane biology and chemistry
-Cell biology
-Biophysics
-Genetic expression
-Pharmacological properties of food ingredients with an emphasis on the content of bioactive ingredients in foods
Examples of topics covered in recently-published papers on two topics of current wide interest, nutraceuticals/functional foods and postharvest/postmortem, include the following:
-Bioactive compounds found in foods, such as chocolate and herbs, as they affect serum cholesterol, diabetes, hypertension, and heart disease
-The mechanism of the ripening process in fruit
-The biogenesis of flavor precursors in meat
-How biochemical changes in farm-raised fish are affecting processing and edible quality
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