Journal of Food Biochemistry最新文献

{"title":"Kaempferol Ameliorates Renal Remodelling by Inhibiting the Renin-Angiotensin System Cascade in Hypertensive Rats","authors":"Putcharawipa Maneesai,&nbsp;Metee Iampanichakul,&nbsp;Prapassorn Potue,&nbsp;Juthamas Khamseekaew,&nbsp;Terdthai Tong-Un,&nbsp;Parichat Prachaney,&nbsp;Wannapa Settheetham-Ishida,&nbsp;Poungrat Pakdeechote","doi":"10.1155/2024/8810152","DOIUrl":"https://doi.org/10.1155/2024/8810152","url":null,"abstract":"<div>\u0000 <p><i>Background</i>. Kaempferol is a natural flavonoid with a wide range of pharmacological effects. The current study tested whether kaempferol prevents hypertension-induced renal remodelling in rats. During the 5 weeks of experiments, rats (<i>n</i> = 7/group) were administered <i>N</i>_<i>ω</i>-nitro-L-arginine methyl ester hydrochloride (L-NAME) (40 mg/kg/day) with either vehicle or kaempferol (20 mg/kg/day) or kaempferol (40 mg/kg/day) or lisinopril (5 mg/kg/day). <i>Results</i>. Kaempferol treatment alleviated haemodynamic changes occurring in hypertensive rats, including increases in systolic and diastolic blood pressure, pulse pressure, mean arterial pressure, and heart rate (<i>p</i> &lt; 0.05). Kaempferol treatment prevented glomerular hypertrophy by reducing the increased glomerular cross-sectional area, glomerular tuft area, Bowman’s space area, glomerular volume, and the extent of renal tubulointerstitial fibrosis induced by hypertension (<i>p</i> &lt; 0.05). Furthermore, animals in the L-NAME group showed elevated angiotensin-converting enzyme (ACE) activity and angiotensin II (Ang II) levels compared to those in the kaempferol-treated group (<i>p</i> &lt; 0.05). Kaempferol treatment also reverted the elevations in levels of superoxide anions and malondialdehyde and reduced catalase and superoxide dismutase activity in hypertensive rats (<i>p</i> &lt; 0.05). L-NAME-treated rats showed overexpression of angiotensin II receptor type 1 (AT1), nicotinamide adenine dinucleotide phosphate oxidase 4 (NOX4), and matrix metalloproteinase-9 (MMP-9) proteins; conversely, the expression of these proteins was reduced in the kaempferol-treated group (<i>p</i> &lt; 0.05). <i>Conclusion</i>. Kaempferol treatment alleviated renal remodelling induced in rats by chronic hypertension. These mechanisms may be associated with the inhibition of ACE activity and suppression of the Ang II/AT1 receptor/NOX4/MMP-9 cell signalling pathway in renal tissue.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2024 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2024/8810152","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141968276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Holothuria tubulosa Extract Represses Proliferation and HIF-α Activity by Inhibiting Erk1/2 Phosphorylation in Liver Cancer Cell Line under Hypoxia","authors":"Christina Befani,&nbsp;Persephoni Giannouli,&nbsp;Aikaterini Diseri,&nbsp;Anastasia Bari,&nbsp;Chrysoula Apostologamvrou,&nbsp;Chryssanthi Antoniadou,&nbsp;Panagiotis Liakos,&nbsp;Dimitris Vafidis","doi":"10.1155/2024/9278021","DOIUrl":"https://doi.org/10.1155/2024/9278021","url":null,"abstract":"<div>\u0000 <p>Holothurians are prominent marine organisms in benthic ecosystems, exploited as food for centuries and, recently, as nutraceuticals. Moreover, they constitute a significant source of beneficial and medicinally valuable metabolites that have anticancer properties. Hypoxia, or low oxygen levels, and the stimulation of HIFs as a result of rapid cell proliferation and a restricted blood supply are characteristics shared by the majority of cancers, which give them resistance to standard treatment. The present study examines the effect of <i>Holothuria tubulosa</i> extract on the proliferation, HIF-<i>α</i> protein expression, and their transcriptional activity in hepatocarcinoma-derived Huh7 cells. Specimens of <i>H. tubulosa</i> were collected from the Aegean Sea, and their extract decreased the proliferation in Huh7 cells under normoxia and hypoxia in a dose-dependent manner by MTT assay. The extract modified HIF-1<i>α</i> protein expression by western blot analysis, downregulated hypoxia-induced VEGF promoter by luciferase assay, and decreased the expression of known HIF-1 and HIF-2-specific target genes, PGK and EPO, as revealed by real-time PCR. <i>H. tubulosa</i> extract had the ability to reduce the phosphorylated form of ERK1/2 explaining its inhibitory effect on proliferation and HIF activity. These data reveal new roles of <i>H. tubulosa</i> with promising anticancer properties in liver cancer cells.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2024 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2024/9278021","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141968150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Onopordopicrin from the Japanese Leaf Burdock Exerts Antiallergic Effects through the Inhibition of I Kappa B Kinase β","authors":"Akihiro Maeta,&nbsp;Honoka Ishikawa,&nbsp;Yuka Okamoto,&nbsp;Kyoko Takahashi","doi":"10.1155/2024/3999202","DOIUrl":"https://doi.org/10.1155/2024/3999202","url":null,"abstract":"<div>\u0000 <p>Onopordopicrin (OPP), found in burdock leaves and stems, exerts antiallergic properties whose mechanism remains elusive. We aimed to elucidate its mechanism using rat basophilic leukemia cells <i>in vitro</i>. Purified OPP demonstrated concentration-dependent inhibition of degranulation after allergen or PMA/A23187 stimulation. OPP effectively suppressed TNF-<i>α</i> and PGD2 releases. However, OPP did not suppress the increase in intracellular Ca²⁺ concentration after allergen stimulation. The <i>α</i>, <i>β</i>-unsaturated carbonyl structure of OPP suggests potential electrophilic reactivity with polyfunctional thiol-trapping agents, such as cysteine residues. Indeed, the degranulation-inhibiting effect of OPP disappeared with the addition of cysteamine, which possesses a thiol group. I kappa B kinase <i>β</i> (IKK<i>β</i>), which regulates degranulation in an NF-<i>κ</i>B-independent manner, possesses a cysteine residue between the activation loop. Moreover, IKK<i>β</i> plays an important role in TNF-<i>α</i> and PGD2 production. OPP was found to reduce IKK<i>β</i> activity in a concentration-dependent manner. Together, our findings suggest that OPP exerts its antiallergic action via binding to cysteine residues in signal proteins such as IKK<i>β</i>, thereby inhibiting their activation.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2024 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2024/3999202","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141631212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Network Pharmacology Prediction and Experimental Validation of Ferulic Acid’s Protective Effects against Diclofenac-Induced Liver Injury","authors":"Fatema S. Alatawi,&nbsp;Awatif M. E. Omran,&nbsp;Mohsen S. Alatawi,&nbsp;Eman Rashad,&nbsp;Noha A. E. Yasin,&nbsp;Ahmed F. Soliman","doi":"10.1155/2024/5592390","DOIUrl":"https://doi.org/10.1155/2024/5592390","url":null,"abstract":"<div>\u0000 <p>Despite being one of the most consumed analgesics worldwide, liver injury is an adverse effect of diclofenac (DF). In pursuit of reliable hepatoprotective natural remedies, this study aimed to investigate the potential protective effect of ferulic acid (FA) and its mechanism against DF-induced liver injury. Various network databases and datasets were used to collect targets corresponding to FA and DF-induced liver injury. Enrichment analyses of common targets were performed, a protein-protein interaction (PPI) network was constructed, the hub genes were identified, and the upstream miRNA interacting with the top hub gene was later predicted. A DF-induced liver injury rat model was established to verify FA’s protective effects, and the selected hub gene expression level with its upstream regulatory miRNA and a downstream set of targets was examined to elucidate the underlying mechanism. A total of 18 genes were identified as potential targets of FA to protect against DF-induced liver injury. Data from the enrichment and PPI analyses and the prediction of the upstream miRNAs indicated that the most worthwhile pair to study was miR-296-5p/<i>Jun</i>. <i>In vivo</i> findings showed that coadministration of FA significantly reduced the DF-induced alterations in the liver function indices, oxidative stress, and liver histology. Mechanistically, FA downregulated the expression of <i>Jun</i>, <i>Bim</i>, <i>Bax</i>, <i>Casp3</i>, IL-1<i>β</i>, IL-6, and TNF-<i>α</i>, whereas it upregulated the expression of rno-miR-296-5p and <i>Bcl2</i>. In conclusion, combining network pharmacology and an <i>in vivo</i> study revealed that miR-296-5p/<i>Jun</i> axis could mediate the mitigative effect of FA against DF-induced liver injury.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2024 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2024/5592390","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141631160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Abelmoschus esculentus Improves Hippocampal Function Associated with Dipeptidyl Peptidase-4 in High Fat Diet-Fed db/db Mice","authors":"Chiung-Huei Peng,&nbsp;Chau-Jong Wang,&nbsp;Yen-Yu Yang,&nbsp;Chien-Ning Huang,&nbsp;Huei-Jane Lee","doi":"10.1155/2024/5554538","DOIUrl":"https://doi.org/10.1155/2024/5554538","url":null,"abstract":"<div>\u0000 <p>Hippocampal function can be impaired by diabetes mellitus (DM) and obesity. <i>Abelmoschus esculentus</i> (AE) fractions reportedly mitigate the symptoms of Alzheimer’s disease (AD) by downregulating dipeptidyl peptidase-4 (DPP-4)-mediated insulin resistance. AE extracted by alcohol (fraction 1, F1) and water (fraction 2, F2) contained quercetin glycosides and polysaccharides, respectively. In this study, we investigated whether AE affects hippocampal function in <i>in vitro</i> and <i>in vivo</i> systems. Our results indicate that F1 or F2 enhanced neurogenesis and synapse formation in palmitate-treated hippocampal neural cells, presumably by downregulating DPP-4. In db/db mice fed with high fat diet, the hippocampal insulin resistance correlated spatial recognition, with fraction F2 improving hippocampal function. Of note, the alteration of neurogenesis seems interconnecting with changes in gut microbiota. In summary, AE can improve hippocampal function, attenuate insulin resistance, and promote neurogenesis by regulating DPP-4. AE, particularly F2, has the potential to serve as an adjuvant in preventing DM-associated AD.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2024 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2024/5554538","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141631213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antiviral Activity of Curcuminoids Derived from Deverra tortuosa Plant","authors":"Eman Elsharkawy,&nbsp;May Alqurashi,&nbsp;Mona Alshathly,&nbsp;A. K. M. Moyeenul Huq","doi":"10.1155/2024/5548871","DOIUrl":"https://doi.org/10.1155/2024/5548871","url":null,"abstract":"<div>\u0000 <p>Curcuminoids are natural products with widespread biological activity mostly extracted from <i>Curcuma longa</i> and its family. In the current study, we report the first time to isolate curcuminoids from another phyto source. Three compounds (curcumin, desmethoxycurcumin (DMC), and bisdemethoxycurcumin (BDMC)) were isolated from the plant Deverra tortuosa using methanolic extract, which was then fractionated and separated by puriFlash. The purity of compounds was monitored by thin-layer chromatography and confirmed by high-performance liquid chromatography. The mass spectrometry identified the isolated compound by their electrospray ionisation. Molecular docking then demonstrated that curcumin and BDMC bind at the same cavity (187 Å) on SARS-CoV-2 M^pro protein indicating similarities while DMC binds to a cavity with a larger size (372 Å). Among the three curcuminoids, BDMC was shown to give the highest binding energy (−7.5 kcal/mol) followed by curcumin (−7.4 kcal/mol) and DMC (−7.3 kcal/mol). Calculating the half-maximal cytotoxic concentration and inhibitory median concentration of both DMC and BDMC showed low-to-moderate antiviral activity against SARS-CoV-2.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2024 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2024/5548871","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141608008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Edible Bird’s Nest on Protecting against Cognitive Deficit and Ameliorating Beta-Amyloid in Hippocampal Rats’ Model of Cerebral Ischemia","authors":"Nualpun Sirinupong,&nbsp;Worapanit Chansuwan,&nbsp;Pratchaya Kaewkaen","doi":"10.1155/2024/7367894","DOIUrl":"https://doi.org/10.1155/2024/7367894","url":null,"abstract":"<div>\u0000 <p>Cerebral ischemia has been identified as the primary cause of global mortality, but there is currently no effective therapy for treating this condition. Consequently, the search for novel neuroprotective agents that can guard against stroke remains essential. Recent research has demonstrated the importance of alternative treatments in the pathology of cerebral ischemia leading to vascular dementia. The purpose of this study was to determine the effect of neurocognitive behavior and beta-amyloid markers in terms of the neuroprotective effects of Edible Bird’s Nest (EBN). Male adult Wistar rats were orally administered EBN at concentrations of 10, 50, and 100 mg/kg for 2 weeks prior to and 3 weeks after middle cerebral artery occlusion surgery to mimic vascular dementia. The rodents were then classified based on their neurological score, beta-amyloid accumulation, volume of cerebral infarction, and acetylcholinesterase activity. We assessed the animals’ spatial memory and administered the Morris water maze and radial arm maze tests. We found that EBN substantially decreased beta-amyloid in the hippocampus, decreased acetylcholinesterase activity, and enhanced the animals’ neurological scores and spatial memory. We conclude that EBN contains potential substances that promote learning and memory pathways. However, additional research is still necessary to confirm these findings.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2024 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2024/7367894","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141565814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Glucosamine Hydrochloride and Glucosamine-Gallic Acid Nanoparticles for the Treatment of Osteoarthritis: Synthesis, Antioxidant, and Anti-Inflammatory","authors":"Alika Jafari,&nbsp;Mehdi Tabarsa,&nbsp;Hossein Naderi-Manesh,&nbsp;Hassan Ahmadi Gavlighi,&nbsp;SangGuan You,&nbsp;Zahra Vaezi","doi":"10.1155/2024/3272099","DOIUrl":"https://doi.org/10.1155/2024/3272099","url":null,"abstract":"<div>\u0000 <p>This study aimed to evaluate the antioxidant and anti-inflammatory effects of glucosamine nanoparticles (GNPs) grafted with gallic acid (GNPs-<i>g</i>-GA). Glucosamine hydrochloride (G-HCl) was produced from shrimp shell, and then GNPs synthesized using ionic gelation method. GNPs-<i>g</i>-GA was prepared by coupling GNPs with GA via 1-ethy-3-(3-dimethylaminopropyl)-carbodiimide (EDC) in combination with N-hydroxysuccinimide (NHS) cross-linking agents. The results indicated that the grafting of GA onto GNPs at different ratios increased the average size of the nanoparticles from 195.7 to 294.2 nm with various grafting degrees ranging from 73.3 to 146.4 mg GA/g GNPs-<i>g</i>-GA. The SEM images revealed the formation of spherical-shaped GNPs-<i>g</i>-GA nanoparticles with approximate sizes ranging from 275.3 to 303.6 nm. The appearance of characteristic signals in the FT-IR (C=C, C–O/C–C, and NH₂) and ¹H-NMR (H-2 and H-6 at 6.95 ppm) spectra and the red shift in UV-Vis spectrum provided further support of GNPs-<i>g</i>-GA successful synthesis. DPPH radical scavenging (from 20.0 to 70.4%) and ABTS radical scavenging (from 18.7 to 79.0%) activities and reducing power (nearly fivefold) sharply improved in GNPs-<i>g</i>-GA. Moreover, GNPs-<i>g</i>-GA was found nontoxic and drastically reduced the level of nitric oxide release and downregulated the synthesis of TNF-<i>α</i>, IL-1<i>β</i>, and IL-6 in LPS-induced RAW2647 murine macrophage cells through NF-<i>κ</i>B and MAPKs signaling pathways. Overall, these results suggested that the grafting of GNPs and GA is an effective strategy for the suppression of inflammation response and oxidation reaction in osteoarthritis.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2024 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2024/3272099","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141487790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effects of Chia Supplementation on Lipid Profile in Patients Suffering from Metabolic Disorders: A Systematic Review and Meta-Analysis","authors":"Qi Run,&nbsp;Haoming Yan,&nbsp;Pedram Pam,&nbsp;Parsa Jamilian,&nbsp;Meysam Zarezadeh,&nbsp;Hongyan Zhang","doi":"10.1155/2024/5587140","DOIUrl":"https://doi.org/10.1155/2024/5587140","url":null,"abstract":"<div>\u0000 <p><i>Background</i>. Dyslipidemia is a major public health challenge worldwide, and chia seed, a nutrient-dense functional food, could be beneficial in metabolic disorders. This systematic review and meta-analysis were conducted to assess how chia seed intake affects lipid profiles. <i>Methods</i>. A comprehensive literature search was conducted on electronic databases including PubMed, Scopus, Web of Science, Cochrane, and Google Scholar up to January 2024. All randomized controlled trials (RCTs) addressing the effect of chia seed on triglyceride (TG), total cholesterol (TC), high-density lipoprotein (HDL), and low-density lipoprotein (LDL) were included. Data were analyzed using a random-effects model and reported as weighted mean differences (WMD) with 95% confidence intervals (CI). Subgroup and sensitivity analyses were also performed. The quality of studies was evaluated using the Cochrane risk of bias tool. <i>Results</i>. A total of seven RCTs involving 304 participants were included in the meta-analysis. The results showed that chia consumption had no considerable effect on TG (WMD: −18.73 mg/dl; 95% CI: −55.46 to 18.00; <i>p</i> = 0.31), TC (WMD: −7.49 mg/dl; 95% CI: −15.60 to 0.63; <i>p</i> = 0.07), HDL (WMD: −2.85 mg/dl; 95% CI: −6.74 to 1.05; <i>p</i> = 0.15), and LDL (WMD: −8.09 mg/dl; 95% CI: −18.17 to 1.99; <i>p</i> = 0.11). However, subgroup analyses indicated that chia could decrease TC in patients with obesity and type 2 diabetes mellitus (T2DM) and LDL in patients with obesity. <i>Conclusion</i>. Chia consumption had no effect on lipid profiles including triglyceride, total cholesterol, LDL, and HDL. Nevertheless, patients with obesity and T2DM might benefit from its supplementation.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2024 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2024/5587140","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141488347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Screening of Functional Lactic Acid Bacteria with Potential Production of Antimicrobial Peptides from Milk Proteins for Application as Preservative Dairy Starter","authors":"Khaled Elsaadany,&nbsp;Ehab Kheadr,&nbsp;Sarah Elmesseiry,&nbsp;Saeed Hamdy,&nbsp;Nassra Dabour","doi":"10.1155/2024/7634462","DOIUrl":"https://doi.org/10.1155/2024/7634462","url":null,"abstract":"<div>\u0000 <p>This study aimed to assess the molecular screening and safety of 14 lactic acid bacteria isolates for their capacity to produce antimicrobial peptides from milk proteins. The isolates were molecularly identified as <i>Enterococcus faecium</i>, <i>Enterococcus durans</i>, <i>Lactococcus lactis</i> subsp. <i>lactis</i>, <i>Lactococcus lactis</i> subsp. <i>hordniae</i>, <i>Lactiplantibacillus plantarum</i>, and <i>Weissella confusa</i>. The tested lactococci isolates produced bioactive peptides with a wild-type antimicrobial spectrum against <i>Salmonella typhimurium</i>, <i>Pseudomonas aeruginosa</i>, <i>Bacillus cereus</i>, and <i>Listeria monocytogenes</i>. The majority of isolates were susceptible to chloramphenicol, tetracycline, and ampicillin but resistant to nalidixic acid. <i>Lactococcus lactis</i> FFNL-2034 and <i>Weissella confusa</i> FFNL-1850 presented antibiotic multiresistance. Antibiotic resistance genes <i>bla</i>, <i>ermB</i>, <i>tetK</i>, <i>cat</i>, <i>vanA</i>, and <i>vanC</i> were absent in most of the tested isolates. The <i>ermC</i>, <i>strA</i>, <i>strB,</i> and <i>tetM</i> genes were positive in some isolates. These results provide safe LAB, which could act as a potent biopreservative agent for functional dairy products.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2024 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2024/7634462","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141488557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}