Journal of Food Biochemistry最新文献

{"title":"Antifatigue Effects of Deer-Hide Gelatin on Mice by Regulating Nrf2/Keap1 and AMPK/PGC1α Signaling Pathways and Intestinal Flora","authors":"Yuan Chang,&nbsp;Lulu Zhang,&nbsp;Zixuan Nie,&nbsp;Yixiang Miao,&nbsp;Tianzhu Jia,&nbsp;Ji Shi,&nbsp;Pengpeng Liu","doi":"10.1155/jfbc/6652369","DOIUrl":"https://doi.org/10.1155/jfbc/6652369","url":null,"abstract":"<div>\u0000 <p><b>Objective:</b> This study investigated the antifatigue effects of deer-hide gelatin (DHG) and its mechanism in mice through a weight-loaded swimming experiment.</p>\u0000 <p><b>Methods:</b> The subjects were assigned to the blank group (BC), positive group (PC), model group (MC), and high, medium, and low doses of DHG groups (HP, MP, and LP). After 4 weeks of treatment, the subjects were sacrificed to detect fatigue-related biochemical indicators and the protein and mRNA expressions of Nrf2/Keap1 and AMPK/PGC1<i>α</i> pathways. The morphological changes of skeletal muscle were detected. High-throughput sequencing technology was used to detect the changes in the relative abundance of intestinal flora and the content of short-chain fatty acids (SCFAs) in tired subjects.</p>\u0000 <p><b>Results:</b> Compared with MC, DHG could prolong the exhaustion time of weight-loaded swimming mice; reduce the CK, BUN, lactic acid, MDA, 5-HT, and GABA levels; and increase the LDH, SOD, CAT, Glycogen, MG, BG, ACH, and Glu levels. Moreover, DHG increased the protein and mRNA expression of Nrf2, HO-1, AMPK, PGC1<i>α</i>, and P-AMPK and reduced the protein and mRNA expression of Keap1. The 16S rDNA sequencing analysis also showed that DHG regulated the abundance of intestinal microbiota and the content of SCFAs and increased the growth of beneficial bacteria.</p>\u0000 <p><b>Conclusions:</b> DHG exhibited antifatigue effects on mice by activating Nrf2/Keap1 and AMPK/PGC1<i>α</i> pathways, reducing oxidative stress damage, and enhancing mitochondrial energy supply. The study’s findings confirmed the considerable antioxidant and antifatigue activities of DHG, providing a preliminary foundation and practical theory for the further development of DHG as a nutritional supplement.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2024 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/6652369","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142762088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Different Drying Methods on Drying Kinetic Models, Physicochemical Properties, and Drying Quality Composite Scores of Rosa roxburghii Tratt (Fruit)","authors":"Wei Ren,&nbsp;Jian Xu,&nbsp;Yongping Zhang,&nbsp;Yanyan Miao,&nbsp;Yao Liu","doi":"10.1155/jfbc/3900785","DOIUrl":"https://doi.org/10.1155/jfbc/3900785","url":null,"abstract":"<div>\u0000 <p>The aim of this study is to investigate efficient, cost-effective, and high-quality drying technologies for <i>Rosa roxburghii</i> Tratt. Five drying methods—hot air drying (HAD), microwave drying (MD), vacuum drying (VD), microwave vacuum drying (MVD), and freeze vacuum drying (FVD), were employed to dry <i>Rosa roxburghii</i> Tratt. The study focused on evaluating drying characteristics, colors, texture and structure, and microstructure, as well as the content of active ingredients and antioxidant activity of <i>Rosa roxburghii</i> Tratt. The results of drying kinetics indicated that the Wang and Singh model was the most suitable for FVD, while the Page model was optimal for the other four drying methods. <i>Rosa roxburghii</i> Tratt subjected to HAD exhibited the highest energy consumption per unit but the lowest <i>ΔE</i> and the highest TFC. The microstructure of <i>Rosa roxburghii</i> Tratt under different drying methods was analyzed. <i>Rosa roxburghii</i> Tratt dried using MD experienced serious cell rupture, resulting in a large loss of active ingredients and the worst antioxidant ability, but it had the fastest drying rate. On the other hand, <i>Rosa roxburghii</i> Tratt dried under VD had the microstructure with the least pore space, leading to slower drying speed and the highest total polyphenol content. <i>Rosa roxburghii</i> Tratt dried using MVD had the least hardness and chewiness, with slightly better Δ<i>E</i> and active ingredient content compared to MD. FVD <i>Rosa roxburghii</i> Tratt took the longest time to dry, with the greatest Δ<i>E</i> difference, hardness, and chewiness, but it had the highest Vitamin C content and the most powerful antioxidant ability. Principal component analysis (PCA)–entropy weighting method was used to provide comprehensive scores for the different drying methods, with FVD, HAD, VD, MVD, and MD were 0.8107, 0.7037, 0.4499, 0.2746, and 0.0586 respectively. Through comprehensive analysis, we demonstrated that the quality of <i>Rosa roxburghii</i> Tratt obtained via FVD and HAD is satisfactory. Additionally, HAD is characterized by its economic efficiency and convenience, providing valuable insights for the industrial production of <i>Rosa roxburghii</i> Tratt drying.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2024 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/3900785","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142749363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"γ-Glutamylcysteine Inhibits VSMC-Derived Foam Cell Formation via Upregulating Thioredoxin-1 Expression","authors":"Jianzhen Lei,&nbsp;Jinyi Zhou,&nbsp;Fen Zheng,&nbsp;Yang Yang,&nbsp;Qiuyue Wu,&nbsp;Xinyi Xia,&nbsp;Lan Luo","doi":"10.1155/jfbc/2360339","DOIUrl":"https://doi.org/10.1155/jfbc/2360339","url":null,"abstract":"<div>\u0000 <p>The formation and accumulation of foam cells within the arterial wall is the main early event of atherosclerosis. Vascular smooth muscle cells (VSMCs) are the most abundant cells in human atherosclerotic lesions and are suggested to contribute to atheroma foam cells. <i>γ</i>-glutamylcysteine (<i>γ</i>-GC), as an immediate precursor of glutathione, exhibits anti-inflammatory and antioxidant effects. However, the effects of <i>γ</i>-GC on the formation of atherosclerotic plaques are not yet clarified. Here, we display the effects of <i>γ</i>-GC against ox-LDL-induced formation of foamy VSMCs. Our results showed that <i>γ</i>-GC apparently reduced the total intracellular cholesterol content in mouse and rat VSMCs. The oil red O and fluorescent BODIPY staining indicated that <i>γ</i>-GC inhibited the formation of foam cell. Mechanistically, <i>γ</i>-GC reduced the expression of macrophage scavenger receptor 1 (Msr1) in VSMCs to attenuate cholesterol uptake by the cells. Further RNA-seq analysis, and immunofluorescence as well as immunoblotting measurements showed that thioredoxin-1 (Txn1) was important for <i>γ</i>-GC to reduce Msr1 expression in VSMCs and lipid uptake by the cells. Overall, our research indicated that <i>γ</i>-GC increases Txn1 expression, which causes scavenger receptor A reduction and lipid uptake inhibition in ox-LDL-stimulated VSMCs. Our study revealed that <i>γ</i>-GC has potential for the prevention of atherosclerosis.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2024 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/2360339","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142737617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pharmacological Appraisal of Aqueous Ethanolic Extract of Cuminum cyminum (Cumin Seeds) in Obesity and Endocrinopathies Associated With Polycystic Ovarian Syndrome in Animal Models","authors":"Wenwen Wang,&nbsp;Muhammad Qasim Barkat,&nbsp;Sehrish Amin,&nbsp;Abdulrahman Alshammari,&nbsp;Norah A. Albekairi,&nbsp;Hina Manzoor,&nbsp;Zunera Chauhdary,&nbsp;Rizwan Rashid Bazmi,&nbsp;Liaqat Hussain,&nbsp;Qin Chen","doi":"10.1155/jfbc/1184854","DOIUrl":"https://doi.org/10.1155/jfbc/1184854","url":null,"abstract":"<div>\u0000 <p>A multidisciplinary endocrinopathy affecting women in their reproductive years, polycystic ovarian syndrome, causes insulin resistance, hyperandrogenism, cardiovascular issues, obesity, and menstrual difficulties. <i>Cuminum cyminum</i> (cumin seed) is a famous spice traditionally used for various ailments such as indigestion, headache, diarrhea, obesity, and gynecological disorders. This study explored the pharmacological potential of aqueous ethanolic extract of <i>Cuminum cyminum</i> (AECC) in letrozole-induced PCOS animals, which were also obese. HPLC analysis revealed the presence of chlorogenic acid, gallic acid, hydroxybenzoic acid (HB acid), caffeic acid, vanillic acid, kaempferol, sinapic acid, ferulic acid, salicylic acid, coumarin, quercetin, benzoic acid, rutin, and <i>p</i>-coumaric acid. Thirty adult female rats were divided into six groups (5 animals in each group). PCOS was induced by using letrozole (1 mg/kg). The groups were normal control, diseased control, standard (metformin, 20 mg/kg), and AECC (200, 400, and 600 mg/kg). Vaginal smear cytology was used to confirm the PCOS. The animals were euthanized after 12 weeks, and blood was drawn for hormonal and biochemical study. We used isoflurane 5% for euthanasia in a closed chamber. The ovaries were removed for histopathology. Ovarian histopathology showed abnormalities with cystic follicles in PCOS female rats. It also produced hyperinsulinemia, LH surge, lower FSH, perturbed LH/FSH ratio, and higher levels of testosterone and weight gain. Moreover, it also produced oxidative stress with ROS (reactive oxygen species) production. Meanwhile, AECC treatment converted cystic follicles into primary, secondary, and differentiating follicles with healthy granulosa cells and corpus luteum with significant (<i>p</i> &lt; 0.001) weight loss. AECC significantly (<i>p</i> &lt; 0.001) reduced serum insulin levels and normalized LH/FSH ratio, with improved FSH levels and control of LH surge. AECC also reduced oxidative stress and lipid peroxidation with significant (<i>p</i> &lt; 0.001) improvement of antioxidant markers. It has been concluded that AEEC can alleviate PCOS symptoms by improving insulin resistance, reducing weight, addressing hyperandrogenism, regulating the LH/FSH ratio, and overcoming oxidative stress and ROS production.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2024 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/1184854","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142708129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Flavonoids Isolated From Delphinium semibarbatum Flowering Aerial Parts With Their Antibacterial, Antibiofilm, and Antiswarming Activity Against Proteus mirabilis and Staphylococcus aureus","authors":"Mohammadreza Lotfaliani,&nbsp;Seyed Abdulmajid Ayatollahi,&nbsp;Farzad Kobarfard,&nbsp;Mohammad Bagher Majnooni,&nbsp;Arezoo Mirzaei,&nbsp;Mustafa Ghanadian,&nbsp;Mohammad Hossain Farzaei","doi":"10.1155/jfbc/9994944","DOIUrl":"https://doi.org/10.1155/jfbc/9994944","url":null,"abstract":"<div>\u0000 <p><i>Proteus mirabilis</i> and <i>Staphylococcus aureus</i> are pathogens associated with CAUTIs. Understanding their significance is crucial for effective prevention and treatment. In this study, the <i>Delphinium semibarbatum</i> flavonoid-rich fraction (DSF) was analyzed, and its antibacterial, antibiofilm, and antiswarming properties against these two bacterial species were evaluated. In the phytochemical analysis, four flavonoids, including kaempferol, 4′-O-methyl quercetin, quercetin, and kaempferol-3-O-beta-D-glucopyranoside (K3G), were isolated. The minimum inhibitory concentration (MIC₅₀) of DSF on <i>P. mirabilis</i> and <i>S. aureus</i> was 1000 <i>μ</i>g/mL. Similarly, kaempferol, K3G, and 4′-O-methyl quercetin exhibited inhibitory effects of 20%, 13%, and 12%, respectively, at a concentration of 1000 <i>μ</i>g/mL compared to the <i>P. mirabilis</i> control. However, the MIC₅₀ of kaempferol and 4′-O-methyl quercetin for <i>S. aureus</i> were 125 <i>μ</i>g/mL and 62.25 <i>μ</i>g/mL, respectively. K3G exhibited an inhibitory effect of 37% at a concentration of 1000 <i>μ</i>g/mL. DSF reduced biofilm at 1 mg/mL by 78% and 74% for <i>P. mirabilis</i> and <i>S. aureus</i>, respectively. Quercetin was the most effective in inhibiting <i>P. mirabilis</i> biofilm formation, with a 96% inhibition rate, followed by kaempferol and K3G, with 67% and 29%, respectively, at the same concentration. The best bioactive compound against biofilm inhibition of <i>S. aureus</i> was quercetin at 250 <i>μ</i>g/mL, exhibiting 90% inhibition, followed by K3G, 4′-O-methyl quercetin, and kaempferol with 81%, 45%, and 33%, respectively, at a concentration of 125 <i>μ</i>g/mL. In the swarming inhibition assay, DSF showed 78% inhibition at concentrations of 5 <i>μ</i>g/mL. Similarly, all bioactive compounds showed 45% antiswarming activity at this concentration. The docking results of the isolated flavonoids on two target proteins, SarA and MrpH, showed that the presence of the methoxy group in the structure of 4′-O-methyl quercetin reduced its interaction with these two proteins. In the ADMET prediction, isolated flavonoids showed good predicted properties suitable for treating these two microbial species in urinary infection diseases.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2024 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/9994944","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142708298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Herbacetin Attenuates Oxidative Stress via Activating Nrf2/HO-1 Signaling Pathway in RAW 264.7 Cells and Caenorhabditis elegans","authors":"Wei Wang,&nbsp;Jia-Qi Luo,&nbsp;Bing-Yi Lv,&nbsp;Zhan-Wei Dong,&nbsp;Chong-Shuo Zhai,&nbsp;Yu-Han Hu,&nbsp;Zhen Jin,&nbsp;Dan Du,&nbsp;You-Zhi Tang","doi":"10.1155/jfbc/5515410","DOIUrl":"https://doi.org/10.1155/jfbc/5515410","url":null,"abstract":"<div>\u0000 <p>Flavonoids have long been used as food additives due to their antioxidant properties. To discover bioactive natural flavonoids, the antioxidant capabilities of a range of natural flavonoids were investigated in RAW 264.7 cells and <i>Caenorhabditis elegans</i> induced by H₂O₂. Chemical antioxidant activity of a range of flavonoids was performed by DPPH and ABTS⁺ radicals scavenging assays. Then, the cellular antioxidant activity assays were employed to detect the antioxidant mechanisms by flow cytometry and qRT-PCR analyses. The underlying antioxidant mechanisms were explored by western blotting, small interfering RNA (siRNA), molecular docking, and cellular thermal shift assay (CETSA). Next, the antioxidant potential of active compounds was verified in H₂O₂-induced <i>Caenorhabditis elegans</i>. Our studies showed herbacetin exhibited the most pronounced DPPH and ABTS ⁺ radicals scavenging capacity among all the tested flavonoids. And herbacetin also showed improved antioxidant activity against H₂O₂-induced oxidative stress in RAW 264.7 cells compared to quercetin and Trolox. The structure-activity relationship (SAR) analysis indicated that hydroxyl groups at the 7′ and 8′ positions on the A-ring of herbacetin played a crucial role in inhibiting H₂O₂-induced cellular oxidative stress. Interestingly, herbacetin’s activation of HO-1 was closely associated with its binding to Keap1. Notably, in vivo assays demonstrated that herbacetin protected <i>Caenorhabditis elegans</i> from H₂O₂-induced oxidative stress. This study screens and elucidates the excellent antioxidant capacity of herbacetin both in vitro and in vivo. These findings suggest that herbacetin holds promise as a natural agent for antioxidant therapy.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2024 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/5515410","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142708218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-Inflammatory and Bacteriostatic Effects of Hydrogen-Rich Water on Rats With Periodontitis","authors":"Yang Bai,&nbsp;Lisheng Zhao,&nbsp;Lin Wang,&nbsp;Yanbo Shan,&nbsp;Wei Zhang,&nbsp;Ning Wen","doi":"10.1155/jfbc/5917799","DOIUrl":"https://doi.org/10.1155/jfbc/5917799","url":null,"abstract":"<div>\u0000 <p><b>Objective:</b> To investigate the anti-inflammatory and bacteriostatic effects of hydrogen-rich water (HRW) intake on Wistar rats with periodontitis.</p>\u0000 <p><b>Methods:</b> The periodontitis model of rats was established by silk ligature and <i>Porphyromonas gingivalis</i> (Pg) solution smearing. The rats were divided into normal control group, model group, and HRW group. The rats in normal control group were given normal feeding without any intervention, while periodontitis was induced in the rats of model group and HRW group, and the rats were given HRW intake and normal saline, respectively, during the whole procedure of the experiments. The antibacterial effect and clinical symptoms (plaque index and gingival sulcus bleeding index (SBI)) were then observed, and peripheral blood (PB), gingival sulcus fluid, and jaw samples were collected to evaluate the changes of inflammatory factors and the curative effect.</p>\u0000 <p><b>Results:</b> In the rats of model group, alveolar bone resorption was obvious, which indicated that the rats with periodontitis were successfully constructed, and HRW intake mitigated it. Pg colony number of the rats fed with HRW was significantly lower than that of the model rats. The contents of proinflammatory factors tumor necrosis factor alpha (TNF-<i>α</i>), interleukin-1 beta (IL-1<i>β</i>), and interleukin-6 (IL-6) in PB serum and gingival crevicular fluid (GCF) of rats in model group were significantly increased, and HRW intake reversed these and promoted the level of anti-inflammatory factor interleukin-10 (IL-10).</p>\u0000 <p><b>Conclusion:</b> HRW can effectively inhibit the growth of periodontal pathogens and reduce inflammation and may be used to prevent or ameliorate periodontitis.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2024 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/5917799","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142708230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Screening and Characterization of Potential Acetylcholinesterase Inhibitors From Phyllanthus emblica L. Fruits Using Affinity Ultrafiltration Combined With Ultra-High-Performance Liquid Chromatography–Mass Spectrometry","authors":"Yangzom Dawa,&nbsp;Juan Chen","doi":"10.1155/jfbc/6643716","DOIUrl":"https://doi.org/10.1155/jfbc/6643716","url":null,"abstract":"<div>\u0000 <p>In this study, targeting acetylcholinesterase (AChE) related to Alzheimer’s disease, a screening method, affinity ultrafiltration combined with ultra-high-performance liquid chromatography–quadrupole-time-of-flight mass spectrometry (AUF–UHPLC–Q-TOF MS) was developed for the discovery and identification of AChE inhibitors from <i>Phyllanthus emblica</i> L. fruits, a medicinal and food homologous plant. The 30% ethanol extract of <i>P</i>. <i>emblica</i> fruit was incubated with AChE allowing active components to form complexes with AChE. Subsequently, the complexes were separated from the incubation and dissociated to release active components, followed by UHPLC–Q-TOF MS analysis. Ultimately, a total of 18 compounds bound to AChE were screened out and identified. Among them, elaeocarpusin, putranjivain A, and chebulagic acid were confirmed to possess the highest affinity to AChE by molecular docking. Subsequently, the AChE inhibitory activity of commercially available chebulagic acid and corilagin was verified in vitro. Ultimately, cellular assays demonstrated that both chebulagic acid and corilagin enhanced cell viability in a concentration-dependent manner when compared to a model of AD cells induced by A<i>β</i>_25–35. Moreover, it was noted that chebulagic acid exhibited superior protective effects relative to corilagin against A<i>β</i>_25–35-induced injury in PC12 cells. These findings indicate that the developed methodology is not only straightforward and rapid but also reliable, offering significant insights for the screening of active compounds from complex medicinal and food homologous plants.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2024 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/6643716","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142691329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Applying an Untargeted Metabolomic Strategy to Explore the Interventional Effect and Mechanism of NMN on Aging Model Mice","authors":"Yuxian Lin,&nbsp;Yan Wu,&nbsp;Yuanying Zhu,&nbsp;Ting Luo,&nbsp;Zheng Sun,&nbsp;Xuecun Liu,&nbsp;Yingcong Yu,&nbsp;Hui Xu","doi":"10.1155/2024/8472130","DOIUrl":"https://doi.org/10.1155/2024/8472130","url":null,"abstract":"<div>\u0000 <p><b>Background:</b> Aging is usually accompanied by a significant decline in nicotinamide adenine dinucleotide (NAD⁺) level. Nicotinamide mononucleotide (NMN) is a crucial precursor molecule of NAD⁺ with a variety of bioactivities beneficial to health, and the present study just aimed to explore the antiaging effects of NMN and the mechanism of action by using a nontargeted metabolomic strategy.</p>\u0000 <p><b>Methods:</b> An aging mouse model induced by D-galactosamine (D-gal) was established, which was followed by treatment with NMN (at doses of 100 to 500 mg/kg per day) via oral administration for 10 weeks. The physiological and biochemical changes involved in the aging process were closely monitored to investigate the interventional effects of NMN. Plasma samples were subjected to assay by UPLC-Q-Orbitrap HRMS to reveal the mechanism of action via systematic data mining and statistical analysis.</p>\u0000 <p><b>Results:</b> D-gal injection at a dose of 500 mg/kg per day for 7 weeks successfully induced the aging model in mice. Long-term supplementation with NMN could significantly improve the antioxidant and immune function, boost lipid metabolism, and reduce aging-related inflammatory response in a dose- and time-dependent manner. The nontargeted metabolomic analysis further demonstrated that the interventional effect of NMN on aging mice may be closely related to the regulation of lipid and purine metabolism.</p>\u0000 <p><b>Conclusions:</b> Long-term NMN supplementation could display robust antiaging effects mainly by regulating lipid and purine metabolism, which thus deserves great attention for antiaging intervention.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2024 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2024/8472130","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142685255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phenolic Profiling and Inhibitory Activities of Cholinesterase and β-Amyloid Aggregation in Ziziphus Species Fruit Fractions","authors":"Ezzouhra El Maaiden","doi":"10.1155/jfbc/4695284","DOIUrl":"https://doi.org/10.1155/jfbc/4695284","url":null,"abstract":"<div>\u0000 <p>In recent years, there has been growing interest in the potential of medicinal plants for preventing and treating neurodegenerative diseases. This study investigated the neuroprotective properties of aqueous extracts derived from the pulp, seed, and almonds of <i>Ziziphus spina-christi (L.) Desf.</i> and <i>Ziziphus lotus (L.) Lam.</i> These extracts were assessed in vitro for their impact on acetylcholinesterase, butyrylcholinesterase, and <i>β</i>-amyloid aggregation, all key factors associated with neurodegenerative disorders (NDs). Given the strong connection between NDs and oxidative damage, as well as the role of metal accumulation in exacerbating oxidative stress and amyloid plaque formation, the antioxidant abilities of the extracts were also evaluated using various methods including ferric-reducing antioxidant power, cupric-reducing antioxidant capacity, and metal chelating assays. Furthermore, the study analyzed the total phenolic and flavonoid contents using standard spectrophotometric techniques, and the secondary metabolite composition was examined through UHPLC-DAD analysis. To better understand the relationship between different plant species and their organ types with specific phenolic compounds and biological activities, principal component analysis (PCA) was employed for data analysis. Results revealed the presence of 25 secondary metabolites categorized into phenolic and flavonoid compounds. Interestingly, the seed extracts exhibited the highest bioactivity, significantly inhibiting antioxidant and cholinergic enzymes. In addition, almond extracts displayed promising antiamyloidogenic properties, effectively suppressing oligomers, fibrillation of A<i>β</i>42, and the amyloid cascade. In conclusion, this study highlights the potential of seed and almond extracts from <i>Z. spina-christi (L.) Desf.</i> and <i>Z. lotus (L.) Lam.</i> as valuable sources of natural compounds with neuroprotective properties against neurodegenerative diseases.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2024 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/4695284","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142692058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}