Synergistic Protection of Alpha-Glucosyl Hesperidin and Procyanidin Against Testicular Ischemia-Reperfusion Injury

IF 3.5 2区农林科学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of Food Biochemistry Pub Date : 2025-02-24 DOI:10.1155/jfbc/5634210

Ehsan Zeynali, Ali Soleimanzadeh, Saeed Azizi

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Abstract

The present study was designed to examine the protective effects of alpha-glucosyl hesperidin (AGH) and procyanidin (PC) against testicular ischemia/reperfusion (I/R) injury, which is a severe medical condition. Seven groups of male rats were divided into control, torsion/detorsion (T/D), T/D+50 mg/kg PC, T/D+25 mg/kg AGH, T/D+50 mg/kg AGH, T/D+50 mg/kg PC+25 mg/kg AGH, and T/D+50 mg/kg PC+50 mg/kg AGH. The evaluation of testicular tissue, sperm quality, oxidative status, gene activity, and fertility levels after T/D was conducted using various tests. The results showed that sperm motility, viability, and plasma membrane functionality, as well as total antioxidant capacity (TAC), superoxide dismutase (SOD), catalase (CAT), and Bcl-2 gene expression levels, all increased after treatment with AGH or PC and coadministration. Additionally, the levels of glutathione (GSH), malondialdehyde (MDA), Bax, and caspase-3 gene expression and abnormal morphology and DNA damage decreased compared to the T/D group. The study found that AGH and PC had a more substantial synergistic effect when used together to improve reproductive health following T/D damage.

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本研究旨在探讨α-葡萄糖基橙皮甙（AGH）和原花青素（PC）对睾丸缺血再灌注损伤（I/R）的保护作用。七组雄性大鼠被分为对照组、扭转/离体（T/D）组、T/D+50 mg/kg PC组、T/D+25 mg/kg AGH组、T/D+50 mg/kg AGH组、T/D+50 mg/kg PC+25 mg/kg AGH组和T/D+50 mg/kg PC+50 mg/kg AGH组。通过各种测试对T/D后的睾丸组织、精子质量、氧化状态、基因活性和生育力水平进行了评估。结果显示，使用 AGH 或 PC 及联合用药治疗后，精子活力、存活率、质膜功能以及总抗氧化能力（TAC）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和 Bcl-2 基因表达水平均有所提高。此外，与T/D组相比，谷胱甘肽（GSH）、丙二醛（MDA）、Bax和Caspase-3基因表达水平以及异常形态和DNA损伤均有所下降。研究发现，当 AGH 和 PC 一起用于改善 T/D 损伤后的生殖健康时，会产生更显著的协同效应。

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来源期刊

Journal of Food Biochemistry 生物-生化与分子生物学

CiteScore

7.80

自引率

5.00%

发文量

488

审稿时长

3.6 months

期刊介绍： The Journal of Food Biochemistry publishes fully peer-reviewed original research and review papers on the effects of handling, storage, and processing on the biochemical aspects of food tissues, systems, and bioactive compounds in the diet. Researchers in food science, food technology, biochemistry, and nutrition, particularly based in academia and industry, will find much of great use and interest in the journal. Coverage includes: -Biochemistry of postharvest/postmortem and processing problems -Enzyme chemistry and technology -Membrane biology and chemistry -Cell biology -Biophysics -Genetic expression -Pharmacological properties of food ingredients with an emphasis on the content of bioactive ingredients in foods Examples of topics covered in recently-published papers on two topics of current wide interest, nutraceuticals/functional foods and postharvest/postmortem, include the following: -Bioactive compounds found in foods, such as chocolate and herbs, as they affect serum cholesterol, diabetes, hypertension, and heart disease -The mechanism of the ripening process in fruit -The biogenesis of flavor precursors in meat -How biochemical changes in farm-raised fish are affecting processing and edible quality