Journal of Developmental Biology最新文献_第3页

Phase Separation as a Driver of Stem Cell Organization and Function during Development. 相分离是发育过程中干细胞组织和功能的驱动力

IF 2.7

Journal of Developmental Biology Pub Date : 2023-12-12 DOI: 10.3390/jdb11040045

Amalia S Parra, Christopher A Johnston

{"title":"Phase Separation as a Driver of Stem Cell Organization and Function during Development.","authors":"Amalia S Parra, Christopher A Johnston","doi":"10.3390/jdb11040045","DOIUrl":"10.3390/jdb11040045","url":null,"abstract":"A properly organized subcellular composition is essential to cell function. The canonical organizing principle within eukaryotic cells involves membrane-bound organelles; yet, such structures do not fully explain cellular complexity. Furthermore, discrete non-membrane-bound structures have been known for over a century. Liquid-liquid phase separation (LLPS) has emerged as a ubiquitous mode of cellular organization without the need for formal lipid membranes, with an ever-expanding and diverse list of cellular functions that appear to be regulated by this process. In comparison to traditional organelles, LLPS can occur across wider spatial and temporal scales and involves more distinct protein and RNA complexes. In this review, we discuss the impacts of LLPS on the organization of stem cells and their function during development. Specifically, the roles of LLPS in developmental signaling pathways, chromatin organization, and gene expression will be detailed, as well as its impacts on essential processes of asymmetric cell division. We will also discuss how the dynamic and regulated nature of LLPS may afford stem cells an adaptable mode of organization throughout the developmental time to control cell fate. Finally, we will discuss how aberrant LLPS in these processes may contribute to developmental defects and disease.","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":"11 4","pages":""},"PeriodicalIF":2.7,"publicationDate":"2023-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10743522/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138830038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Special Nuclear Structures in the Germinal Vesicle of the Common Frog with Emphasis on the So-Called Karyosphere Capsule. 普通青蛙生殖泡中的特殊核结构，重点是所谓的 "核膜囊"。

IF 2.7

Journal of Developmental Biology Pub Date : 2023-12-12 DOI: 10.3390/jdb11040044

Dmitry S Bogolyubov, Sergey V Shabelnikov, Alexandra O Travina, Maksim I Sulatsky, Irina O Bogolyubova

{"title":"Special Nuclear Structures in the Germinal Vesicle of the Common Frog with Emphasis on the So-Called Karyosphere Capsule.","authors":"Dmitry S Bogolyubov, Sergey V Shabelnikov, Alexandra O Travina, Maksim I Sulatsky, Irina O Bogolyubova","doi":"10.3390/jdb11040044","DOIUrl":"10.3390/jdb11040044","url":null,"abstract":"The karyosphere (karyosome) is a structure that forms in the oocyte nucleus-germinal vesicle (GV)-at the diplotene stage of meiotic prophase due to the assembly of all chromosomes in a limited portion of the GV. In some organisms, the karyosphere has an extrachromosomal external capsule, the marker protein of which is nuclear F-actin. Despite many years of theories about the formation of the karyosphere capsule (KC) in the GV of the common frog Rana temporaria, we present data that cast doubt on its existence, at least in this species. Specific extrachromosomal strands, which had been considered the main elements of the frog's KC, do not form a continuous layer around the karyosphere and, according to immunogold labeling, do not contain structural proteins, such as actin and lamin B. At the same time, F-actin is indeed noticeably concentrated around the karyosphere, creating the illusion of a capsule at the light microscopy/fluorescence level. The barrier-to-autointegration factor (BAF) and one of its functional partners-LEMD2, an inner nuclear membrane protein-are not localized in the strands, suggesting that the strands are not functional counterparts of the nuclear envelope. The presence of characteristic strands in the GV of R. temporaria late oocytes may reflect an excess of SMC1 involved in the structural maintenance of diplotene oocyte chromosomes at the karyosphere stage, since SMC1 has been shown to be the most abundant protein in the strands. Other characteristic microstructures-the so-called annuli, very similar in ultrastructure to the nuclear pore complexes-do not contain nucleoporins Nup35 and Nup93, and, therefore, they cannot be considered autonomous pore complexes, as previously thought. Taken together, our data indicate that traditional ideas about the existence of the R. temporaria KC as a special structural compartment of the GV are to be revisited.","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":"11 4","pages":""},"PeriodicalIF":2.7,"publicationDate":"2023-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10744300/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138830039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Epidermal Barrier Development via Corneoptosis: A Unique Form of Cell Death in Stratum Granulosum Cells. 通过角质凋亡发展表皮屏障：角质层细胞的一种独特细胞死亡形式

IF 2.7

Journal of Developmental Biology Pub Date : 2023-11-30 DOI: 10.3390/jdb11040043

Takeshi Matsui

{"title":"Epidermal Barrier Development via Corneoptosis: A Unique Form of Cell Death in Stratum Granulosum Cells.","authors":"Takeshi Matsui","doi":"10.3390/jdb11040043","DOIUrl":"10.3390/jdb11040043","url":null,"abstract":"Epidermal development is responsible for the formation of the outermost layer of the skin, the epidermis. The establishment of the epidermal barrier is a critical aspect of mammalian development. Proper formation of the epidermis, which is composed of stratified squamous epithelial cells, is essential for the survival of terrestrial vertebrates because it acts as a crucial protective barrier against external threats such as pathogens, toxins, and physical trauma. In mammals, epidermal development begins from the embryonic surface ectoderm, which gives rise to the basal layer of the epidermis. This layer undergoes a series of complex processes that lead to the formation of subsequent layers, including the stratum intermedium, stratum spinosum, stratum granulosum, and stratum corneum. The stratum corneum, which is the topmost layer of the epidermis, is formed by corneoptosis, a specialized form of cell death. This process involves the transformation of epidermal keratinocytes in the granular layer into flattened dead cells, which constitute the protective barrier. In this review, we focus on the intricate mechanisms that drive the development and establishment of the mammalian epidermis to gain insight into the complex processes that govern this vital biological system.","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":"11 4","pages":""},"PeriodicalIF":2.7,"publicationDate":"2023-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10744242/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138830037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SARS-CoV-2 Infection in Late Pregnancy and Childbirth from the Perspective of Perinatal Pathology. 从围产期病理学角度看妊娠晚期和分娩时SARS-CoV-2感染

IF 2.7

Journal of Developmental Biology Pub Date : 2023-11-16 DOI: 10.3390/jdb11040042

Larisa Debelenko

{"title":"SARS-CoV-2 Infection in Late Pregnancy and Childbirth from the Perspective of Perinatal Pathology.","authors":"Larisa Debelenko","doi":"10.3390/jdb11040042","DOIUrl":"10.3390/jdb11040042","url":null,"abstract":"This review focuses on SARS-CoV-2 infection in placental and fetal tissues. Viremia is rare in infected pregnant women, and the virus is seldom amplified from placental tissues. Definite and probable placental infection requires the demonstration of viral RNA or proteins using in situ hybridization (ISH) and immunohistochemistry (IHC). Small subsets (1.0-7.9%, median 2.8%) of placentas of SARS-CoV-2-positive women showed definite infection accompanied by a characteristic histopathology named SARS-CoV-2 placentitis (SP). The conventionally accepted histopathological criteria for SP include the triad of intervillositis, perivillous fibrin deposition, and trophoblast necrosis. SP was shown to be independent of the clinical severity of the infection, but associated with stillbirth in cases where destructive lesions affecting more than 75% of the placental tissue resulted in placental insufficiency and severe fetal hypoxic-ischemic injury. An association between maternal thrombophilia and SP was shown in a subset of cases, suggesting a synergy of the infection and deficient coagulation cascade as one of the mechanisms of the pathologic accumulation of fibrin in affected placentas. The virus was amplified from fetal tissues in approximately 40% of SP cases, but definite fetal involvement demonstrated using ISH or IHC is exceptionally rare. The placental pathology in SARS-CoV-2-positive women also includes chronic lesions associated with placental malperfusion in the absence of definite or probable placental infection. The direct viral causation of the vascular malperfusion of the placenta in COVID-19 is debatable, and common predispositions (hypertension, diabetes, and obesity) may play a role.","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":"11 4","pages":""},"PeriodicalIF":2.7,"publicationDate":"2023-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10660738/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138176347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Andy Golden: Mentorship through the Years 安迪·戈尔登:多年的导师

Journal of Developmental Biology Pub Date : 2023-11-03 DOI: 10.3390/jdb11040041

Anna K. Allen, Xiaofei Bai, Edward S. Davis, Amy Fabritius, Aimee Jaramillo-Lambert, Peter A. Kropp, Christopher T. Richie, Jill M. Schumacher, Sanjay Shrestha, Kathryn Stein, Ann K. Corsi

引用次数: 0

Use of Farnesyl Transferase Inhibitors in an Ageing Model in Drosophila 法尼基转移酶抑制剂在果蝇衰老模型中的应用

Journal of Developmental Biology Pub Date : 2023-10-29 DOI: 10.3390/jdb11040040

Annely Brandt, Roman Petrovsky, Maria Kriebel, Jörg Großhans

{"title":"Use of Farnesyl Transferase Inhibitors in an Ageing Model in Drosophila","authors":"Annely Brandt, Roman Petrovsky, Maria Kriebel, Jörg Großhans","doi":"10.3390/jdb11040040","DOIUrl":"https://doi.org/10.3390/jdb11040040","url":null,"abstract":"The presence of farnesylated proteins at the inner nuclear membrane (INM), such as the Lamins or Kugelkern in Drosophila, leads to specific changes in the nuclear morphology and accelerated ageing on the organismal level reminiscent of the Hutchinson–Gilford progeria syndrome (HGPS). Farnesyl transferase inhibitors (FTIs) can suppress the phenotypes of the nuclear morphology in cultured fibroblasts from HGPS patients and cultured cells overexpressing farnesylated INM proteins. Similarly, FTIs have been reported to suppress the shortened lifespan in model organisms. Here, we report an experimental system combining cell culture and Drosophila flies for testing the activity of substances on the HGPS-like nuclear morphology and lifespan, with FTIs as an experimental example. Consistent with previous reports, we show that FTIs were able to ameliorate the nuclear phenotypes induced by the farnesylated nuclear proteins Progerin, Kugelkern, or truncated Lamin B in cultured cells. The subsequent validation in Drosophila lifespan assays demonstrated the applicability of the experimental system: treating adult Drosophila with the FTI ABT-100 reversed the nuclear phenotypes and extended the lifespan of experimentally induced short-lived flies. Since kugelkern-expressing flies have a significantly shorter average lifespan, half the time is needed for testing substances in the lifespan assay.","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":"47 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136135846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The New Nematicide Cyclobutrifluram Targets the Mitochondrial Succinate Dehydrogenase Complex in Caenorhabditis elegans. 新型线虫环丁氟仑靶向秀丽隐杆线虫线粒体琥珀酸脱氢酶复合体。

IF 2.7

Journal of Developmental Biology Pub Date : 2023-10-19 DOI: 10.3390/jdb11040039

Fariba Heydari, David Rodriguez-Crespo, Chantal Wicky

{"title":"The New Nematicide Cyclobutrifluram Targets the Mitochondrial Succinate Dehydrogenase Complex in Caenorhabditis elegans.","authors":"Fariba Heydari, David Rodriguez-Crespo, Chantal Wicky","doi":"10.3390/jdb11040039","DOIUrl":"10.3390/jdb11040039","url":null,"abstract":"Today, agriculture around the world is challenged by parasitic nematode infections. Plant-parasitic nematodes (PPNs) can cause significant damage and crop loss and are a threat to food security. For a long time, the management of PPN infection has relied on nematicides that impact not only parasitic nematodes but also other organisms. More recently, new nematicides have been developed that appear to specifically target PPN. Cyclobutrifluram belongs to this new category of nematicides. Using the nematode Caenorhabditis elegans as a model organism, we show here that cyclobutrifluram strongly impacts the survival and fertility rates of the worm by decreasing the number of germ cells. Furthermore, using a genetic approach, we demonstrate that cyclobutrifluram functions by inhibiting the mitochondrial succinate dehydrogenase (SDH) complex. Transcriptomic analysis revealed a strong response to cyclobutrifluram exposure. Among the deregulated genes, we found genes coding for detoxifying proteins, such as cytochrome P450s and UDP-glucuronosyl transferases (UGTs). Overall, our study contributes to the understanding of the molecular mode of action of cyclobutrifluram, to the finding of new approaches against nematicide resistance, and to the discovery of novel nematicides. Furthermore, this study confirms that C. elegans is a suitable model organism to study the mode of action of nematicides.","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":"11 4","pages":""},"PeriodicalIF":2.7,"publicationDate":"2023-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10594496/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49690788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regulation and Function of FOXC1 in Osteoblasts. FOXC1在成骨细胞中的调节和功能。

IF 2.7

Journal of Developmental Biology Pub Date : 2023-09-19 DOI: 10.3390/jdb11030038

Sarocha Suthon, Jianjian Lin, Rachel S Perkins, Gustavo A Miranda-Carboni, Susan A Krum

{"title":"Regulation and Function of FOXC1 in Osteoblasts.","authors":"Sarocha Suthon, Jianjian Lin, Rachel S Perkins, Gustavo A Miranda-Carboni, Susan A Krum","doi":"10.3390/jdb11030038","DOIUrl":"https://doi.org/10.3390/jdb11030038","url":null,"abstract":"Estrogens, which bind to estrogen receptor alpha (ERα), are important for proper bone mineral density. When women go through menopause, estrogen levels decrease, and there is a decrease in bone quality, along with an increased risk for fractures. We previously identified an enhancer near FOXC1 as the most significantly enriched binding site for estrogen receptor alpha (ERα) in osteoblasts. FOXC1 is a transcription factor belonging to a large group of proteins known as forkhead box genes and is an important regulator of bone formation. Here, we demonstrate that 17β-estradiol (E2) increases the mRNA and protein levels of FOXC1 in primary mouse and human osteoblasts. GATA4 is a pioneer factor for ERα and it is also recruited to enhancers near Foxc1. Knockdown of Gata4 in mouse osteoblasts in vitro decreases Foxc1 expression as does knockout of Gata4 in vivo. Functionally, GATA4 and FOXC1 interact and regulate osteoblast proteins such as RUNX2, as demonstrated by ChIP-reChIP and luciferase assays. The most enriched motif in GATA4 binding sites from ChIP-seq is for FOXC1, supporting the notion that GATA4 and FOXC1 cooperate in regulating osteoblast differentiation. Together, these data demonstrate the interactions of the transcription factors ERα, GATA4, and FOXC1 to regulate each other's expression and other osteoblast differentiation genes.","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":"11 3","pages":""},"PeriodicalIF":2.7,"publicationDate":"2023-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10531946/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41115526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identifying Molecular Roadblocks for Transcription Factor-Induced Cellular Reprogramming In Vivo by Using C. elegans as a Model Organism. 以秀丽隐杆线虫为模型生物鉴定转录因子诱导的体内细胞重编程的分子障碍。

IF 2.7

Journal of Developmental Biology Pub Date : 2023-08-31 DOI: 10.3390/jdb11030037

Ismail Özcan, Baris Tursun

{"title":"Identifying Molecular Roadblocks for Transcription Factor-Induced Cellular Reprogramming In Vivo by Using C. elegans as a Model Organism.","authors":"Ismail Özcan, Baris Tursun","doi":"10.3390/jdb11030037","DOIUrl":"https://doi.org/10.3390/jdb11030037","url":null,"abstract":"Generating specialized cell types via cellular transcription factor (TF)-mediated reprogramming has gained high interest in regenerative medicine due to its therapeutic potential to repair tissues and organs damaged by diseases or trauma. Organ dysfunction or improper tissue functioning might be restored by producing functional cells via direct reprogramming, also known as transdifferentiation. Regeneration by converting the identity of available cells in vivo to the desired cell fate could be a strategy for future cell replacement therapies. However, the generation of specific cell types via reprogramming is often restricted due to cell fate-safeguarding mechanisms that limit or even block the reprogramming of the starting cell type. Nevertheless, efficient reprogramming to generate homogeneous cell populations with the required cell type's proper molecular and functional identity is critical. Incomplete reprogramming will lack therapeutic potential and can be detrimental as partially reprogrammed cells may acquire undesired properties and develop into tumors. Identifying and evaluating molecular barriers will improve reprogramming efficiency to reliably establish the target cell identity. In this review, we summarize how using the nematode C. elegans as an in vivo model organism identified molecular barriers of TF-mediated reprogramming. Notably, many identified molecular factors have a high degree of conservation and were subsequently shown to block TF-induced reprogramming of mammalian cells.","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":"11 3","pages":""},"PeriodicalIF":2.7,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10531806/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41115525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Current Advances in Bovine In Vitro Maturation and Embryo Production Using Different Antioxidants: A Review. 使用不同抗氧化剂的牛体外成熟和胚胎生产的最新进展：综述。

IF 2.7

Journal of Developmental Biology Pub Date : 2023-08-29 DOI: 10.3390/jdb11030036

Roksana Naspinska, Maria Helena Moreira da Silva, Fernando Moreira da Silva

引用次数: 0