Journal of Developmental Biology最新文献_第2页

HP1-Mediated Silencing of the Doublesex1 Gene for Female Determination in the Crustacean Daphnia magna. hp1介导的双双ex1基因沉默对大水蚤雌性决定的影响。

IF 2.5

Journal of Developmental Biology Pub Date : 2025-07-03 DOI: 10.3390/jdb13030023

Junya Leim, Nikko Adhitama, Quang Dang Nong, Pijar Religia, Yasuhiko Kato, Hajime Watanabe

{"title":"HP1-Mediated Silencing of the Doublesex1 Gene for Female Determination in the Crustacean Daphnia magna.","authors":"Junya Leim, Nikko Adhitama, Quang Dang Nong, Pijar Religia, Yasuhiko Kato, Hajime Watanabe","doi":"10.3390/jdb13030023","DOIUrl":"10.3390/jdb13030023","url":null,"abstract":"The crustacean Daphnia magna produces genetically identical females and males by parthenogenesis. Males are produced in response to environmental cues including crowding and lack of food. For male development, the DM-domain containing transcription factor Doublesex1 (DSX1) is expressed spatiotemporally in male-specific traits and orchestrates male trait formation in both somatic and gonadal tissues. However, it remains unknown how the dsx1 gene is silenced in females to avoid male trait development. Heterochromatin Protein 1 (HP1) plays a crucial role in epigenetic gene silencing during developmental processes. Here we report the identification of four HP1 orthologs in D. magna. None of these orthologs exhibited sexually dimorphic expression, and among them, HP1-1 was most abundantly expressed during embryogenesis. The knock-down of HP1-1 in female embryos led to the derepression of dsx1 in the male-specific traits, resulting in the development of male characteristics, such as the elongation of the first antennae. These results suggest that HP1-1 silences dsx1 for female development while environmental cues unlock this silencing to induce male production. We infer the HP1-dependent formation of a sex-specific chromatin structure on the dsx1 locus is a key process in the environmental sex determination of D. magna.","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":"13 3","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12286036/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144698703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigating Psychopharmaceutical Effects on Early Vertebrate Development Using a Zebrafish Model System. 利用斑马鱼模型系统研究精神药物对早期脊椎动物发育的影响。

IF 2.5

Journal of Developmental Biology Pub Date : 2025-06-27 DOI: 10.3390/jdb13030022

Nathan Zimmerman, Aaron Marta, Carly Baker, Zeljka Korade, Károly Mirnics, Annemarie Shibata

{"title":"Investigating Psychopharmaceutical Effects on Early Vertebrate Development Using a Zebrafish Model System.","authors":"Nathan Zimmerman, Aaron Marta, Carly Baker, Zeljka Korade, Károly Mirnics, Annemarie Shibata","doi":"10.3390/jdb13030022","DOIUrl":"10.3390/jdb13030022","url":null,"abstract":"Cholesterol homeostasis is necessary for normal vertebrate development. The disruption of cholesterol homeostasis can cause abnormal body and nervous system development and lead to dysfunctional behavior and increased mortality. Commonly prescribed psychopharmaceuticals can alter cholesterol synthesis and may disrupt early vertebrate development. A high-throughput vertebrate zebrafish model system was used to test the hypothesis that exposure to psychopharmaceutical medications alters cholesterol biosynthesis and disrupts gene transcription, early whole-body and brain development, and nervous system function, resulting in abnormal behavior. Exposure to cariprazine, aripiprazole, trazodone, and AY9944 increased 7-dehydrocholesterol levels compared to vehicle-treated zebrafish. Significant differences in disease-associated gene expression, brain structure, and functional behaviors were observed in psychopharmaceutical and AY9944-treated zebrafish compared to controls. These data reveal that the high-throughput zebrafish model system can discern psychopharmaceutical effects on cholesterol synthesis, gene transcription, and key features of early vertebrate development that influences behavior.","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":"13 3","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12286116/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144698704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Drosophila Males Differentially Express Small Proteins Regulating Stem Cell Division Frequency in Response to Mating. 雄性果蝇在交配条件下差异表达调节干细胞分裂频率的小蛋白。

IF 2.5

Journal of Developmental Biology Pub Date : 2025-06-23 DOI: 10.3390/jdb13030021

Manashree S Malpe, Leon F McSwain, Heath M Aston, Karl A Kudyba, Chun Ng, Megan P Wright, Cordula Schulz

{"title":"Drosophila Males Differentially Express Small Proteins Regulating Stem Cell Division Frequency in Response to Mating.","authors":"Manashree S Malpe, Leon F McSwain, Heath M Aston, Karl A Kudyba, Chun Ng, Megan P Wright, Cordula Schulz","doi":"10.3390/jdb13030021","DOIUrl":"10.3390/jdb13030021","url":null,"abstract":"The germline stem cells (GSCs) in the male gonad of Drosophila can increase their division frequency in response to a demand for more sperm caused by repeated mating. However, the molecules and mechanisms regulating and mediating this response have yet to be fully explored. Here, we present the results of a transcriptome analysis comparing expression from the testis tips from non-mated and mated males. An overlapping set of 18 differentially expressed genes (DEGs) from two independent wild-type (wt) strains revealed that the majority of the DEGs encode secreted proteins, which suggests roles for them in cell-cell interactions. Consistent with a role for secretion in regulating GSC divisions, knocking down Signal Recognition Particle (SRP) components within the germline cells using RNA Interference (RNAi), prevented the increase in GSC division frequency in response to mating. The major class of DEGs encodes polypeptides below the size of 250 amino acids, also known as small proteins. Upon reducing germline expression of small proteins, males no longer increased GSC division frequency after repeated mating. We hypothesize that mating induces cellular interactions via small proteins to ensure continued GSC divisions for the production of sperm.","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":"13 3","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12286032/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144698701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cannabinoid Receptor 1 Regulates Zebrafish Renal Multiciliated Cell Development via cAMP Signaling. 大麻素受体1通过cAMP信号调控斑马鱼肾多纤体细胞发育。

IF 2.2

Journal of Developmental Biology Pub Date : 2025-06-17 DOI: 10.3390/jdb13020020

Thanh Khoa Nguyen, Sophia Baker, Julienne Angtuaco, Liana Arceri, Samuel Kaczor, Bram Fitzsimonds, Matthew R Hawkins, Rebecca A Wingert

{"title":"Cannabinoid Receptor 1 Regulates Zebrafish Renal Multiciliated Cell Development via cAMP Signaling.","authors":"Thanh Khoa Nguyen, Sophia Baker, Julienne Angtuaco, Liana Arceri, Samuel Kaczor, Bram Fitzsimonds, Matthew R Hawkins, Rebecca A Wingert","doi":"10.3390/jdb13020020","DOIUrl":"10.3390/jdb13020020","url":null,"abstract":"Endocannabinoid signaling plays a significant role in neurogenesis and nervous system physiology, but its roles in the development of other tissues are just beginning to be appreciated. Previous reports have shown the presence of the key endocannabinoid receptor Cannabinoid receptor 1 (CB1 or Cnr1) in multiciliated (MCC) tissues and its upregulation in kidney diseases, yet the relationship between Cnr1 and renal MCC development is unknown. Here, we report that Cnr1 is essential for cilia development across tissues and regulates renal MCCs via cyclic AMP (cAMP) signaling during zebrafish embryogenesis. Using a combination of genetic and pharmacological studies, we found that the loss of function, agonism and antagonism of cnr1 all lead to reduced mature renal MCC populations. cnr1 deficiency also led to reduced cilia development across tissues, including the pronephros, ear, Kupffer's vesicle (KV), and nasal placode. Interestingly, treatment with the cAMP activator Forskolin (FSK) restored renal MCC defects in agonist-treated embryos, suggesting that cnr1 mediates cAMP signaling in renal MCC development. Meanwhile, treatment with the cAMP inhibitor SQ-22536 alone or with cnr1 deficiency led to reduced MCC populations, suggesting that cnr1 also mediates renal MCC development independently of cAMP signaling. Our findings indicate that cnr1 has a critical role in controlling renal MCC development both via cAMP signaling and an independent pathway, further revealing implications for ciliopathies and renal diseases.","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":"13 2","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12194200/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144484609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ribosome Incorporation Transdifferentiates Chick Primary Cells and Induces Their Proliferation by Secreting Growth Factors. 核糖体掺入使鸡原代细胞转分化并通过分泌生长因子诱导其增殖。

IF 2.2

Journal of Developmental Biology Pub Date : 2025-06-01 DOI: 10.3390/jdb13020019

Shota Inoue, Arif Istiaq, Anamika Datta, Mengxue Lu, Shintaro Nakayama, Kousei Takashi, Nobushige Nakajo, Shigehiko Tamura, Ikko Kawashima, Kunimasa Ohta

{"title":"Ribosome Incorporation Transdifferentiates Chick Primary Cells and Induces Their Proliferation by Secreting Growth Factors.","authors":"Shota Inoue, Arif Istiaq, Anamika Datta, Mengxue Lu, Shintaro Nakayama, Kousei Takashi, Nobushige Nakajo, Shigehiko Tamura, Ikko Kawashima, Kunimasa Ohta","doi":"10.3390/jdb13020019","DOIUrl":"10.3390/jdb13020019","url":null,"abstract":"Previously, we reported that mammalian cells, specifically human dermal fibroblasts (HDFs), could be transdifferentiated by lactic acid bacteria (LAB). Later, we observed that HDFs incorporated LAB-derived ribosomes, forming the ribosome-induced cell clusters (RICs) and transdifferentiating into cells derived from all three germ layers. Based on this insight, we hypothesized that incorporating ribosomes into non-mammalian cells could reveal the universality of this mechanism and open the door to commercial applications. Our current study demonstrates that ribosome incorporation can transdifferentiate chick primary muscle-derived cells (CMCs) into adipocytes, osteoblasts, and chondrocytes. Furthermore, the culture medium supernatant from ribosome-incorporated CMCs was found to significantly enhance CMC's proliferation. RNA-seq analysis revealed that RICs-CMC exhibit increased expression of genes related to multi-lineage cell growth. In addition, we developed a novel technological shift in meat production-the \"CulNet System\"-which replicates organ interactions within mechanical systems for cell-cultured meat production. While significant efforts are still required to implement this technology in a cost-effective manner, we believe that combining the \"CulNet System\" with ribosome-incorporated multipotent cells that have prolonged culture capability could substantially improve the scalability and cost-effectiveness of cultured chicken meat production. This report highlights a promising approach for cell-culture-based meat production, offering a sustainable alternative to traditional methods.","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":"13 2","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12193757/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144484610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cornified Epithelial Teeth of Jawless Vertebrates Contain Proteins Similar to Keratin-Associated Proteins of Mammalian Skin Appendages. 无颌脊椎动物的角质上皮牙齿含有类似于哺乳动物皮肤附属物角蛋白相关蛋白的蛋白质。

IF 2.2

Journal of Developmental Biology Pub Date : 2025-05-19 DOI: 10.3390/jdb13020018

Attila Placido Sachslehner, David A D Parry, Leopold Eckhart

{"title":"Cornified Epithelial Teeth of Jawless Vertebrates Contain Proteins Similar to Keratin-Associated Proteins of Mammalian Skin Appendages.","authors":"Attila Placido Sachslehner, David A D Parry, Leopold Eckhart","doi":"10.3390/jdb13020018","DOIUrl":"10.3390/jdb13020018","url":null,"abstract":"Keratins and keratin-associated proteins (KRTAPs) are the main components of mammalian nails and hair. Comparative genomics and gene expression studies have revealed that keratins are conserved in all vertebrates, whereas KRTAPs exist only in mammals. Recently, we found hair keratin-like cysteine-rich keratins in jawless vertebrates with confirmed expression in the cornified epithelial teeth of the sea lamprey (Petromyzon marinus). Here, we report that KRTAP-like proteins are also present in the horny teeth of the lamprey. Mass spectrometry-based proteomics identified proteins that share features with KRTAPs, such as high contents of cysteine and tyrosine residues, which support intermolecular interactions, and abundant glycine residues, which endow the proteins with flexibility. Genes encoding KRTAP-like proteins are arranged in a cluster in P. marinus, and the presence of at least one KRTAP-like protein is conserved in phylogenetically diverse species of lamprey, including Lampetra fluviatilis, Lethenteron reissneri, Geotria australis, and Mordacia mordax. The KRTAP-like genes of lampreys contain two exons, whereas mammalian KRTAPs have only a single exon. Although KRTAPs and KRTAP-like proteins are products of independent evolution, their common expression in cornified skin appendages suggests that they fulfill similar functions.","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":"13 2","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12101200/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144127782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In Vitro Embryo Culture Impacts Heart Mitochondria in Male Adolescent Sheep. 体外胚胎培养对雄性青春期绵羊心脏线粒体的影响。

IF 2.2

Journal of Developmental Biology Pub Date : 2025-05-13 DOI: 10.3390/jdb13020017

Reza Amanollahi, Stacey L Holman, Ashley S Meakin, Monalisa Padhee, Kimberley J Botting-Lawford, Song Zhang, Severence M MacLaughlin, David O Kleemann, Simon K Walker, Jennifer M Kelly, Skye R Rudiger, I Caroline McMillen, Michael D Wiese, Mitchell C Lock, Janna L Morrison

{"title":"In Vitro Embryo Culture Impacts Heart Mitochondria in Male Adolescent Sheep.","authors":"Reza Amanollahi, Stacey L Holman, Ashley S Meakin, Monalisa Padhee, Kimberley J Botting-Lawford, Song Zhang, Severence M MacLaughlin, David O Kleemann, Simon K Walker, Jennifer M Kelly, Skye R Rudiger, I Caroline McMillen, Michael D Wiese, Mitchell C Lock, Janna L Morrison","doi":"10.3390/jdb13020017","DOIUrl":"10.3390/jdb13020017","url":null,"abstract":"Assisted reproductive technology (ART)such as in vitro embryo culture (IVC), is widely used in human infertility treatments; however, its long-term effects on the cardiac health of offspring remain unclear. This study aimed to determine whether the effects of IVC on cardiac metabolism and associated signaling pathways persist after birth into adolescence. Embryos were either transferred to an intermediate ewe (ET) or cultured in vitro in the absence (IVC) or presence of human serum (IVCHS) with methionine supplementation (IVCHS+M) for 6 days after mating. Naturally mated (NM) ewes were used as controls. Protein expression and hormone concentrations in the left ventricle (LV) were analyzed using Western blot and LC-MS/MS analyses, respectively. IVC was associated with sex-specific alterations in cardiac mitochondria, with males exhibiting reduced mitochondrial abundance. Cardiac protein expression of oxidative phosphorylation (OXPHOS) complexes 1 and 4 was reduced by IVC. Additionally, IVC reduced protein expression of PDK-4 and Mn-SOD in the IVCHS+M group, which may impact energy efficiency and defense against oxidative stress. These changes may predispose IVC offspring to cardiac oxidative stress and mitochondrial dysfunction, particularly in males. This study provides insights into the sex-dependent effects of IVC on cardiac health, emphasizing the importance of evaluating long-term cardiovascular risks associated with IVC protocols.","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":"13 2","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12101424/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144127776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dynamic Changes of Immunoreactive CD34, CD117, and CD41 Hematopoietic Stem Cells in Human Placentas of Different Gestational Ages. 不同胎龄人胎盘中CD34、CD117和CD41造血干细胞免疫反应性的动态变化

IF 2.2

Journal of Developmental Biology Pub Date : 2025-05-09 DOI: 10.3390/jdb13020016

Sanja Jovicic, Ivan R Nikolic, Ljiljana Amidžić, Vesna Ljubojevic, Maja Barudzija, Ranko Skrbic

{"title":"Dynamic Changes of Immunoreactive CD34, CD117, and CD41 Hematopoietic Stem Cells in Human Placentas of Different Gestational Ages.","authors":"Sanja Jovicic, Ivan R Nikolic, Ljiljana Amidžić, Vesna Ljubojevic, Maja Barudzija, Ranko Skrbic","doi":"10.3390/jdb13020016","DOIUrl":"10.3390/jdb13020016","url":null,"abstract":"Background: The process of prenatal hematopoiesis occurs in various anatomical locations, including the placenta. The placenta is not merely a temporary hematopoietic reservoir, but it is one of the key sites for the synthesis of hematopoietic stem cells (HSCs). This study aimed to investigate the presence, distribution, and immunoprofiles of HSCs in the human placenta during different gestational periods. Materials and Methods: Placental samples of different gestational ages (first, second, and third trimesters) were analyzed using classical hematoxylin and eosin staining and immunohistochemical staining for CD34, CD117, and CD41 markers, with HSC quantification through numerical areal density (NA). Results: Highly immunoreactive CD34 HSCs were present in placentas throughout gestation, while highly immunoreactive CD117 and CD41 HSCs were observed during the first two trimesters. In the first trimester, HSCs were found within the lumen of blood vessels and as individual cells in the mesenchyme of chorionic villi. With advancing gestation, the number of HSCs in the mesenchyme of chorionic villi increased. Conclusions: Immunoreactive CD34, CD117, and CD41 cells are present in significant proportions in various parts of the placenta throughout gestation, indicating that the placenta provides a substantial proportion of HSCs for hematopoiesis.","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":"13 2","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12101343/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144127695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Probe Sequencing Analysis of Regenerating Lizard Tails Indicates Crosstalk Among Osteoclasts, Epidermal Cells, and Fibroblasts. 再生蜥蜴尾巴的探针测序分析表明破骨细胞、表皮细胞和成纤维细胞之间存在串扰。

IF 2.2

Journal of Developmental Biology Pub Date : 2025-05-03 DOI: 10.3390/jdb13020015

Darian J Gamble, Samantha Lopez, Melody Yazdi, Toni Castro-Torres, Thomas P Lozito

{"title":"Probe Sequencing Analysis of Regenerating Lizard Tails Indicates Crosstalk Among Osteoclasts, Epidermal Cells, and Fibroblasts.","authors":"Darian J Gamble, Samantha Lopez, Melody Yazdi, Toni Castro-Torres, Thomas P Lozito","doi":"10.3390/jdb13020015","DOIUrl":"10.3390/jdb13020015","url":null,"abstract":"Lizards are distinguished as the only amniotes, and closest relatives of mammals, capable of multilineage epimorphic regeneration. Tail blastemas of green anole lizards (Anolis carolinensis) consist of col3a1+ fibroblastic connective tissue cells enclosed in krt5+ wound epidermis (WE), both of which are required for regeneration. Blastema and WE formation are known to be closely associated with phagocytic cell populations, including macrophages and osteoclasts. However, it remains unclear what specific phagocytic cell types are required to stimulate regeneration. Here, we explicitly assess the roles of osteoclast activity during blastema and WE formation in regenerating lizard tails. First, probe sequencing was performed at regenerative timepoints on fibroblasts isolated based on col3a1 expression toward establishing pathways involved in stimulating blastema formation and subsequent tail regrowth. Next, treatments with osteoclast inhibitor zoledronic acid (ZA) were used to assess the roles of osteoclast activity in lizard tail regeneration and fibroblast signaling. ZA treatment stunted lizard tail regrowth, suggesting osteoclast activity was required for blastema formation and regeneration. Transcriptomic profiling of fibroblasts isolated from ZA-treated and control lizards linked inhibition of osteoclast activity with limitations in fibroblasts to form pro-regenerative extracellular matrix and support WE formation. These results suggest that crosstalk between osteoclasts and fibroblasts regulates blastema and WE formation during lizard tail regeneration.","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":"13 2","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12101308/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144127703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Follicular Fluid from Cows That Express Estrus During a Fixed-Time Artificial Insemination Protocol Promotes Blastocyst Development. 在固定时间人工授精方案中表达发情奶牛的卵泡液促进囊胚发育。

IF 2.2

Journal of Developmental Biology Pub Date : 2025-04-25 DOI: 10.3390/jdb13020014

Audra W Harl, Verónica M Negrón-Pérez, Jacob W Stewart, George A Perry, Alan D Ealy, Michelle L Rhoads

{"title":"Follicular Fluid from Cows That Express Estrus During a Fixed-Time Artificial Insemination Protocol Promotes Blastocyst Development.","authors":"Audra W Harl, Verónica M Negrón-Pérez, Jacob W Stewart, George A Perry, Alan D Ealy, Michelle L Rhoads","doi":"10.3390/jdb13020014","DOIUrl":"10.3390/jdb13020014","url":null,"abstract":"It is not yet understood why cows that exhibit estrus and ovulate are more likely to become pregnant than those that ovulate but do not exhibit estrus during a fixed-time artificial insemination (FTAI) protocol. The objective of this work was to determine whether the follicular fluid from cows that exhibit estrus contributes to the increased likelihood of pregnancy. Lactating crossbred cows were subjected to an FTAI estrous synchronization protocol. Estrous behavior was observed and recorded prior to transvaginal follicle aspiration from cows that did (estrus, n = 7) or did not exhibit estrus (non-estrus, n = 6). Follicular fluid (25%) was then added to in vitro maturation media for the maturation of oocytes (n = 1489) from slaughterhouse ovaries. Cleavage rates were not affected by the estrous status of the cows from which the follicular fluid was collected. Blastocyst rates, however, were greater following maturation in the presence of follicular fluid from estrus cows compared to non-estrus cows (p ≤ 0.01). This difference in blastocyst rates was not related to blastocyst cell numbers (inner cell mass, trophoblast, and total), as they did not differ between estrus and non-estrus animals. This study demonstrates that the follicular fluid, and thus, the follicular environment just prior to ovulation does indeed contribute to improved pregnancy rates following FTAI.","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":"13 2","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12101264/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144127699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0