Journal of Developmental Biology最新文献

How the Oocyte Nucleolus Is Turned into a Karyosphere: The Role of Heterochromatin and Structural Proteins. 卵母细胞核仁如何变成核仁层？异染色质和结构蛋白的作用

IF 2.2

Journal of Developmental Biology Pub Date : 2024-10-18 DOI: 10.3390/jdb12040028

Venera Nikolova, Maya Markova, Ralitsa Zhivkova, Irina Chakarova, Valentina Hadzhinesheva, Stefka Delimitreva

{"title":"How the Oocyte Nucleolus Is Turned into a Karyosphere: The Role of Heterochromatin and Structural Proteins.","authors":"Venera Nikolova, Maya Markova, Ralitsa Zhivkova, Irina Chakarova, Valentina Hadzhinesheva, Stefka Delimitreva","doi":"10.3390/jdb12040028","DOIUrl":"https://doi.org/10.3390/jdb12040028","url":null,"abstract":"Oocyte meiotic maturation includes large-scale chromatin remodeling as well as cytoskeleton and nuclear envelope rearrangements. This review addresses the dynamics of key cytoskeletal proteins (tubulin, actin, vimentin, and cytokeratins) and nuclear envelope proteins (lamin A/C, lamin B, and the nucleoporin Nup160) in parallel with chromatin reorganization in maturing mouse oocytes. A major feature of this reorganization is the concentration of heterochromatin into a spherical perinucleolar rim called surrounded nucleolus or karyosphere. In early germinal vesicle (GV) oocytes with non-surrounded nucleolus (without karyosphere), lamins and Nup160 are at the nuclear envelope while cytoplasmic cytoskeletal proteins are outside the nucleus. At the beginning of karyosphere formation, lamins and Nup160 follow the heterochromatin relocation assembling a new spherical structure in the GV. In late GV oocytes with surrounded nucleolus (fully formed karyosphere), the nuclear envelope gradually loses its integrity and cytoplasmic cytoskeletal proteins enter the nucleus. At germinal vesicle breakdown, lamin B occupies the karyosphere interior while all the other proteins stay at the karyosphere border or connect to chromatin. In metaphase oocytes, lamin A/C surrounds the spindle, Nup160 localizes to its poles, actin and lamin B are attached to the spindle fibers, and cytoplasmic intermediate filaments associate with both the spindle fibers and the metaphase chromosomes.","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":"12 4","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11503394/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142501608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neural Circuit Remodeling: Mechanistic Insights from Invertebrates. 神经回路重塑：无脊椎动物的机制启示

IF 2.2

Journal of Developmental Biology Pub Date : 2024-10-11 DOI: 10.3390/jdb12040027

Samuel Liu, Kellianne D Alexander, Michael M Francis

{"title":"Neural Circuit Remodeling: Mechanistic Insights from Invertebrates.","authors":"Samuel Liu, Kellianne D Alexander, Michael M Francis","doi":"10.3390/jdb12040027","DOIUrl":"https://doi.org/10.3390/jdb12040027","url":null,"abstract":"As nervous systems mature, neural circuit connections are reorganized to optimize the performance of specific functions in adults. This reorganization of connections is achieved through a remarkably conserved phase of developmental circuit remodeling that engages neuron-intrinsic and neuron-extrinsic molecular mechanisms to establish mature circuitry. Abnormalities in circuit remodeling and maturation are broadly linked with a variety of neurodevelopmental disorders, including autism spectrum disorders and schizophrenia. Here, we aim to provide an overview of recent advances in our understanding of the molecular processes that govern neural circuit remodeling and maturation. In particular, we focus on intriguing mechanistic insights gained from invertebrate systems, such as the nematode Caenorhabditis elegans and the fruit fly Drosophila melanogaster. We discuss how transcriptional control mechanisms, synaptic activity, and glial engulfment shape specific aspects of circuit remodeling in worms and flies. Finally, we highlight mechanistic parallels across invertebrate and mammalian systems, and prospects for further advances in each.","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":"12 4","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11503349/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142501609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Delayed Blastocyst Formation Reduces the Quality and Hatching Ability of Porcine Parthenogenetic Blastocysts by Increasing DNA Damage, Decreasing Cell Proliferation, and Altering Transcription Factor Expression Patterns. 通过增加 DNA 损伤、减少细胞增殖和改变转录因子表达模式，延迟囊胚形成可降低猪孤雌生殖囊胚的质量和孵化能力。

IF 2.2

Journal of Developmental Biology Pub Date : 2024-10-01 DOI: 10.3390/jdb12040026

Ling Sun, Yan Wang, Mo Yang, Zhuang-Ju Xu, Juan Miao, Ying Bai, Tao Lin

{"title":"Delayed Blastocyst Formation Reduces the Quality and Hatching Ability of Porcine Parthenogenetic Blastocysts by Increasing DNA Damage, Decreasing Cell Proliferation, and Altering Transcription Factor Expression Patterns.","authors":"Ling Sun, Yan Wang, Mo Yang, Zhuang-Ju Xu, Juan Miao, Ying Bai, Tao Lin","doi":"10.3390/jdb12040026","DOIUrl":"https://doi.org/10.3390/jdb12040026","url":null,"abstract":"The purpose of this study was to investigate the influence of blastocyst formation timing on the quality of porcine embryos derived from parthenogenetic activation. Newly formed blastocysts at days 6, 7, and 8 of culture [termed formation 6, 7, and 8 blastocysts (F6, F7, and F8 blastocysts)] were obtained, and a series of parameters related to the quality of blastocysts, including apoptosis incidents, DNA replication, pluripotent factors, and blastocyst hatching capacity, were assessed. Delayed blastocyst formation (F7 and/or F8 blastocysts) led to increased levels of ROS, DNA damage, and apoptosis while decreasing the mitochondrial membrane potential, DNA replication, Oct4 levels, and numbers of Sox2-positive cells. F7 blastocysts showed a significantly reduced hatching rate compared to F6 blastocysts; however, F8 blastocysts were unable to develop to the hatching stage. Collectively, our findings suggest a negative correlation between delayed blastocyst formation and blastocyst quality.","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":"12 4","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11503403/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142501607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Myotube Guidance: Shaping up the Musculoskeletal System. 肌管引导：塑造肌肉骨骼系统。

IF 2.2

Journal of Developmental Biology Pub Date : 2024-09-17 DOI: 10.3390/jdb12030025

Aaron N Johnson

{"title":"Myotube Guidance: Shaping up the Musculoskeletal System.","authors":"Aaron N Johnson","doi":"10.3390/jdb12030025","DOIUrl":"10.3390/jdb12030025","url":null,"abstract":"Myofibers are highly specialized contractile cells of skeletal muscles, and dysregulation of myofiber morphogenesis is emerging as a contributing cause of myopathies and structural birth defects. Myotubes are the myofiber precursors and undergo a dramatic morphological transition into long bipolar myofibers that are attached to tendons on two ends. Similar to axon growth cones, myotube leading edges navigate toward target cells and form cell-cell connections. The process of myotube guidance connects myotubes with the correct tendons, orients myofiber morphology with the overall body plan, and generates a functional musculoskeletal system. Navigational signaling, addition of mass and volume, and identification of target cells are common events in myotube guidance and axon guidance, but surprisingly, the mechanisms regulating these events are not completely overlapping in myotubes and axons. This review summarizes the strategies that have evolved to direct myotube leading edges to predetermined tendon cells and highlights key differences between myotube guidance and axon guidance. The association of myotube guidance pathways with developmental disorders is also discussed.","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":"12 3","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11417883/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142288787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Roles of the NR2F Family in the Development, Disease, and Cancer of the Lung. NR2F 家族在肺部发育、疾病和癌症中的作用。

IF 2.2

Journal of Developmental Biology Pub Date : 2024-09-10 DOI: 10.3390/jdb12030024

Jiaxin Yang, Wenjing Sun, Guizhong Cui

引用次数: 0

Evolution and Spatiotemporal Expression of ankha and ankhb in Zebrafish. 斑马鱼ankha和ankhb的进化与时空表达

IF 2.2

Journal of Developmental Biology Pub Date : 2024-09-09 DOI: 10.3390/jdb12030023

Nuwanthika Wathuliyadde, Katherine E Willmore, Gregory M Kelly

{"title":"Evolution and Spatiotemporal Expression of ankha and ankhb in Zebrafish.","authors":"Nuwanthika Wathuliyadde, Katherine E Willmore, Gregory M Kelly","doi":"10.3390/jdb12030023","DOIUrl":"10.3390/jdb12030023","url":null,"abstract":"Craniometaphyseal Dysplasia (CMD) is a rare skeletal disorder that can result from mutations in the ANKH gene. This gene encodes progressive anksylosis (ANK), which is responsible for transporting inorganic pyrophosphate (PPi) and ATP from the intracellular to the extracellular environment, where PPi inhibits bone mineralization. When ANK is dysfunctional, as in patients with CMD, the passage of PPi to the extracellular environment is reduced, leading to excess mineralization, particularly in bones of the skull. Zebrafish may serve as a promising model to study the mechanistic basis of CMD. Here, we provide a detailed analysis of the zebrafish Ankh paralogs, Ankha and Ankhb, in terms of their phylogenic relationship with ANK in other vertebrates as well as their spatiotemporal expression patterns during zebrafish development. We found that a closer evolutionary relationship exists between the zebrafish Ankhb protein and its human and other vertebrate counterparts, and stronger promoter activity was predicted for ankhb compared to ankha. Furthermore, we noted distinct temporal expression patterns, with ankha more prominently expressed in early development stages, and both paralogs also being expressed at larval growth stages. Whole-mount in situ hybridization was used to compare the spatial expression patterns of each paralog during bone development, and both showed strong expression in the craniofacial region as well as the notochord and somites. Given the substantial overlap in spatiotemporal expression but only subtle patterning differences, the exact roles of these genes remain speculative. In silico analyses predicted that Ankha and Ankhb have the same function in transporting PPi across the membrane. Nevertheless, this study lays the groundwork for functional analyses of each ankh paralog and highlights the potential of using zebrafish to find possible targeted therapies for CMD.","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":"12 3","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11417794/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142288785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From Germ Cells to Implantation: The Role of Extracellular Vesicles. 从生殖细胞到植入：细胞外囊泡的作用

IF 2.2

Journal of Developmental Biology Pub Date : 2024-08-23 DOI: 10.3390/jdb12030022

Anna Fazzio, Angela Caponnetto, Carmen Ferrara, Michele Purrello, Cinzia Di Pietro, Rosalia Battaglia

{"title":"From Germ Cells to Implantation: The Role of Extracellular Vesicles.","authors":"Anna Fazzio, Angela Caponnetto, Carmen Ferrara, Michele Purrello, Cinzia Di Pietro, Rosalia Battaglia","doi":"10.3390/jdb12030022","DOIUrl":"10.3390/jdb12030022","url":null,"abstract":"Extracellular vesicles represent a large heterogeneous class of near and long-distance intercellular communication mediators, released by both prokaryotic and eukaryotic cells. Specifically, the scientific community has shown growing interest in exosomes, which are nano-sized vesicles with an endosomal origin. Not so long ago, the physiological goal of exosome generation was largely unknown and required more investigation; at first, it was hypothesized that exosomes are able to remove excess, reject and unnecessary constituents from cells to preserve cellular homeostasis. However, thanks to recent studies, the central role of exosomes in regulating cellular communication has emerged. Exosomes act as vectors in cell-cell signaling by their cargo, proteins, lipids, and nucleic acids, and influence physiological and pathological processes. The findings on exosomes are widespread in a large spectrum of biomedical applications from diagnosis and prognosis to therapies. In this review, we describe exosome biogenesis and the current methods for their isolation and characterization, emphasizing the role of their cargo in female reproductive processes, from gametogenesis to implantation, and the potential involvement in human female disorders.","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":"12 3","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11417829/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142288786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lowered GnT-I Activity Decreases Complex-Type N-Glycan Amounts and Results in an Aberrant Primary Motor Neuron Structure in the Spinal Cord. GnT-I 活性降低会减少复合型 N-糖的数量，导致脊髓中的初级运动神经元结构异常。

IF 2.2

Journal of Developmental Biology Pub Date : 2024-08-16 DOI: 10.3390/jdb12030021

Cody J Hatchett, M Kristen Hall, Abel R Messer, Ruth A Schwalbe

{"title":"Lowered GnT-I Activity Decreases Complex-Type N-Glycan Amounts and Results in an Aberrant Primary Motor Neuron Structure in the Spinal Cord.","authors":"Cody J Hatchett, M Kristen Hall, Abel R Messer, Ruth A Schwalbe","doi":"10.3390/jdb12030021","DOIUrl":"10.3390/jdb12030021","url":null,"abstract":"The attachment of sugar to proteins and lipids is a basic modification needed for organismal survival, and perturbations in glycosylation cause severe developmental and neurological difficulties. Here, we investigated the neurological consequences of N-glycan populations in the spinal cord of Wt AB and mgat1b mutant zebrafish. Mutant fish have reduced N-acetylglucosaminyltransferase-I (GnT-I) activity as mgat1a remains intact. GnT-I converts oligomannose N-glycans to hybrid N-glycans, which is needed for complex N-glycan production. MALDI-TOF MS profiles identified N-glycans in the spinal cord for the first time and revealed reduced amounts of complex N-glycans in mutant fish, supporting a lesion in mgat1b. Further lectin blotting showed that oligomannose N-glycans were more prevalent in the spinal cord, skeletal muscle, heart, swim bladder, skin, and testis in mutant fish relative to WT AB, supporting lowered GnT- I activity in a global manner. Developmental delays were noted in hatching and in the swim bladder. Microscopic images of caudal primary (CaP) motor neurons of the spinal cord transiently expressing EGFP in mutant fish were abnormal with significant reductions in collateral branches. Further motor coordination skills were impaired in mutant fish. We conclude that identifying the neurological consequences of aberrant N-glycan processing will enhance our understanding of the role of complex N-glycans in development and nervous system health.","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":"12 3","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11348029/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142072995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Canonical and Non-Canonical Wnt Signaling Generates Molecular and Cellular Asymmetries to Establish Embryonic Axes. 典型和非典型 Wnt 信号产生分子和细胞不对称，从而建立胚轴

IF 2.2

Journal of Developmental Biology Pub Date : 2024-08-02 DOI: 10.3390/jdb12030020

De-Li Shi

{"title":"Canonical and Non-Canonical Wnt Signaling Generates Molecular and Cellular Asymmetries to Establish Embryonic Axes.","authors":"De-Li Shi","doi":"10.3390/jdb12030020","DOIUrl":"10.3390/jdb12030020","url":null,"abstract":"The formation of embryonic axes is a critical step during animal development, which contributes to establishing the basic body plan in each particular organism. Wnt signaling pathways play pivotal roles in this fundamental process. Canonical Wnt signaling that is dependent on β-catenin regulates the patterning of dorsoventral, anteroposterior, and left-right axes. Non-canonical Wnt signaling that is independent of β-catenin modulates cytoskeletal organization to coordinate cell polarity changes and asymmetric cell movements. It is now well documented that components of these Wnt pathways biochemically and functionally interact to mediate cell-cell communications and instruct cellular polarization in breaking the embryonic symmetry. The dysfunction of Wnt signaling disrupts embryonic axis specification and proper tissue morphogenesis, and mutations of Wnt pathway genes are associated with birth defects in humans. This review discusses the regulatory roles of Wnt pathway components in embryonic axis formation by focusing on vertebrate models. It highlights current progress in decoding conserved mechanisms underlying the establishment of asymmetry along the three primary body axes. By providing an in-depth analysis of canonical and non-canonical pathways in regulating cell fates and cellular behaviors, this work offers insights into the intricate processes that contribute to setting up the basic body plan in vertebrate embryos.","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":"12 3","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11348223/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142072994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Planar Cell Polarity Signaling: Coordinated Crosstalk for Cell Orientation. 平面细胞极性信号：细胞定向的协调串扰

IF 2.7

Journal of Developmental Biology Pub Date : 2024-04-29 DOI: 10.3390/jdb12020012

Sandeep Kacker, Varuneshwar Parsad, Naveen Singh, Daria Hordiichuk, Stacy Alvarez, Mahnoor Gohar, Anshu Kacker, Sunil Kumar Rai

{"title":"Planar Cell Polarity Signaling: Coordinated Crosstalk for Cell Orientation.","authors":"Sandeep Kacker, Varuneshwar Parsad, Naveen Singh, Daria Hordiichuk, Stacy Alvarez, Mahnoor Gohar, Anshu Kacker, Sunil Kumar Rai","doi":"10.3390/jdb12020012","DOIUrl":"10.3390/jdb12020012","url":null,"abstract":"The planar cell polarity (PCP) system is essential for positioning cells in 3D networks to establish the proper morphogenesis, structure, and function of organs during embryonic development. The PCP system uses inter- and intracellular feedback interactions between components of the core PCP, characterized by coordinated planar polarization and asymmetric distribution of cell populations inside the cells. PCP signaling connects the anterior-posterior to left-right embryonic plane polarity through the polarization of cilia in the Kupffer's vesicle/node in vertebrates. Experimental investigations on various genetic ablation-based models demonstrated the functions of PCP in planar polarization and associated genetic disorders. This review paper aims to provide a comprehensive overview of PCP signaling history, core components of the PCP signaling pathway, molecular mechanisms underlying PCP signaling, interactions with other signaling pathways, and the role of PCP in organ and embryonic development. Moreover, we will delve into the negative feedback regulation of PCP to maintain polarity, human genetic disorders associated with PCP defects, as well as challenges associated with PCP.","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":"12 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11130840/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141158350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0