Journal of Developmental Biology最新文献_第10页

Genetic Interaction of Thm2 and Thm1 Shapes Postnatal Craniofacial Bone Thm2和Thm1的遗传相互作用形成出生后颅面骨

IF 2.7

Journal of Developmental Biology Pub Date : 2022-05-11 DOI: 10.3390/jdb10020017

E. Bumann, Portia Hahn Leat, Henry H. Wang, Brittany M Hufft-Martinez, Wei Wang, P. Tran

{"title":"Genetic Interaction of Thm2 and Thm1 Shapes Postnatal Craniofacial Bone","authors":"E. Bumann, Portia Hahn Leat, Henry H. Wang, Brittany M Hufft-Martinez, Wei Wang, P. Tran","doi":"10.3390/jdb10020017","DOIUrl":"https://doi.org/10.3390/jdb10020017","url":null,"abstract":"Ciliopathies are genetic syndromes that link skeletal dysplasias to the dysfunction of primary cilia. Primary cilia are sensory organelles synthesized by intraflagellar transport (IFT)—A and B complexes, which traffic protein cargo along a microtubular core. We have reported that the deletion of the IFT-A gene, Thm2, together with a null allele of its paralog, Thm1, causes a small skeleton with a small mandible or micrognathia in juvenile mice. Using micro-computed tomography, here we quantify the craniofacial defects of Thm2−/−; Thm1aln/+ triple allele mutant mice. At postnatal day 14, triple allele mutant mice exhibited micrognathia, midface hypoplasia, and a decreased facial angle due to shortened upper jaw length, premaxilla, and nasal bones, reflecting altered development of facial anterior-posterior elements. Mutant mice also showed increased palatal width, while other aspects of the facial transverse, as well as vertical dimensions, remained intact. As such, other ciliopathy-related craniofacial defects, such as cleft lip and/or palate, hypo-/hypertelorism, broad nasal bridge, craniosynostosis, and facial asymmetry, were not observed. Calvarial-derived osteoblasts of triple allele mutant mice showed reduced bone formation in vitro that was ameliorated by Hedgehog agonist, SAG. Together, these data indicate that Thm2 and Thm1 genetically interact to regulate bone formation and sculpting of the postnatal face. The triple allele mutant mice present a novel model to study craniofacial bone development.","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2022-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49127090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Two Modulators of Skeletal Development: BMPs and Proteoglycans 骨骼发育的两种调节剂：BMP和蛋白聚糖

IF 2.7

Journal of Developmental Biology Pub Date : 2022-04-06 DOI: 10.3390/jdb10020015

Elham Koosha, B. Eames

{"title":"Two Modulators of Skeletal Development: BMPs and Proteoglycans","authors":"Elham Koosha, B. Eames","doi":"10.3390/jdb10020015","DOIUrl":"https://doi.org/10.3390/jdb10020015","url":null,"abstract":"During embryogenesis, skeletal development is tightly regulated by locally secreted growth factors that interact with proteoglycans (PGs) in the extracellular matrix (ECM). Bone morphogenetic proteins (BMPs) are multifunctional growth factors that play critical roles in cartilage maturation and bone formation. BMP signals are transduced from plasma membrane receptors to the nucleus through both canonical Smad and noncanonical p38 mitogen-activated protein kinase (MAPK) pathways. BMP signalling is modulated by a variety of endogenous and exogenous molecular mechanisms at different spatiotemporal levels and in both positive and negative manners. As an endogenous example, BMPs undergo extracellular regulation by PGs, which generally regulate the efficiency of ligand-receptor binding. BMP signalling can also be exogenously perturbed by a group of small molecule antagonists, such as dorsomorphin and its derivatives, that selectively bind to and inhibit the intracellular kinase domain of BMP type I receptors. In this review, we present a current understanding of BMPs and PGs functions in cartilage maturation and osteoblast differentiation, highlighting BMP–PG interactions. We also discuss the identification of highly selective small-molecule BMP receptor type I inhibitors. This review aims to shed light on the importance of BMP signalling and PGs in cartilage maturation and bone formation.","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2022-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47853505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Lizard Blastema Organoid Model Recapitulates Regenerated Tail Chondrogenesis. 蜥蜴类器官母细胞模型再生尾部软骨形成

IF 2.2

Journal of Developmental Biology Pub Date : 2022-02-10 DOI: 10.3390/jdb10010012

Ariel C Vonk, Sarah C Hasel-Kolossa, Gabriela A Lopez, Megan L Hudnall, Darian J Gamble, Thomas P Lozito

{"title":"Lizard Blastema Organoid Model Recapitulates Regenerated Tail Chondrogenesis.","authors":"Ariel C Vonk, Sarah C Hasel-Kolossa, Gabriela A Lopez, Megan L Hudnall, Darian J Gamble, Thomas P Lozito","doi":"10.3390/jdb10010012","DOIUrl":"10.3390/jdb10010012","url":null,"abstract":"(1) Background: Lizard tail regeneration provides a unique model of blastema-based tissue regeneration for large-scale appendage replacement in amniotes. Green anole lizard (Anolis carolinensis) blastemas contain fibroblastic connective tissue cells (FCTCs), which respond to hedgehog signaling to create cartilage in vivo. However, an in vitro model of the blastema has not previously been achieved in culture. (2) Methods: By testing two adapted tissue dissociation protocols and two optimized media formulations, lizard tail FCTCs were pelleted in vitro and grown in a micromass blastema organoid culture. Pellets were analyzed by histology and in situ hybridization for FCTC and cartilage markers alongside staged original and regenerating lizard tails. (3) Results: Using an optimized serum-free media and a trypsin- and collagenase II-based dissociation protocol, micromass blastema organoids were formed. Organoid cultures expressed FCTC marker CDH11 and produced cartilage in response to hedgehog signaling in vitro, mimicking in vivo blastema and tail regeneration. (4) Conclusions: Lizard tail blastema regeneration can be modeled in vitro using micromass organoid culture, recapitulating in vivo FCTC marker expression patterns and chondrogenic potential.","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2022-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8883911/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46232219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic and Molecular Determinants of Lymphatic Malformations: Potential Targets for Therapy 淋巴畸形的遗传和分子决定因素:治疗的潜在目标

IF 2.7

Journal of Developmental Biology Pub Date : 2022-02-08 DOI: 10.3390/jdb10010011

Su Yeon Lee, E. Loll, A. Hassan, Mingyu Cheng, Aijun Wang, D. Farmer

引用次数: 5

Actin Filament in the First Cell Cycle Contributes to the Determination of the Anteroposterior Axis in Ascidian Development 第一个细胞周期中的肌动蛋白丝有助于决定腹水鞘发育的前后轴

IF 2.7

Journal of Developmental Biology Pub Date : 2022-02-04 DOI: 10.3390/jdb10010010

Toshiyuki Goto, Shuhei Torii, Aoi Kondo, Kazumasa Kanda, Junji Kawakami, Y. Kataoka, T. Nishikata

{"title":"Actin Filament in the First Cell Cycle Contributes to the Determination of the Anteroposterior Axis in Ascidian Development","authors":"Toshiyuki Goto, Shuhei Torii, Aoi Kondo, Kazumasa Kanda, Junji Kawakami, Y. Kataoka, T. Nishikata","doi":"10.3390/jdb10010010","DOIUrl":"https://doi.org/10.3390/jdb10010010","url":null,"abstract":"In many animal species, the body axis is determined by the relocalization of maternal determinants, organelles, or unique cell populations in a cytoskeleton-dependent manner. In the ascidian first cell cycle, the myoplasm, including mitochondria, endoplasmic reticulum (ER), and maternal mRNAs, move to the future posterior side concomitantly (called ooplasmic segregation or cytoplasmic and cortical reorganization). This translocation consists of first and second phases depending on the actin and microtubule, respectively. However, the transition from first to second phase, that is, translocation of myoplasmic components from microfilaments to microtubules, has been poorly investigated. In this study, we analyzed the relationship between these cytoskeletons and myoplasmic components during the first cell cycle and their role in morphogenesis by inhibitor experiments. Owing to our improved visualization techniques, there was unexpected F-actin accumulation at the vegetal pole during this transition period. When this F-actin was depolymerized, the microtubule structure was strongly affected, the myoplasmic components, including maternal mRNA, were mislocalized, and the anteroposterior axis formation was disordered. These results suggested the importance of F-actin during the first cell cycle and the existence of interactions between microfilaments and microtubules, implying the enigmatic mechanism of ooplasmic segregation. Solving this mystery leads us to an improved understanding of ascidian early development.","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2022-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44081351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Different Ectopic Hoxa2 Expression Levels in Mouse Cranial Neural Crest Cells Result in Distinct Craniofacial Anomalies and Homeotic Phenotypes 小鼠颅神经嵴细胞中不同的异位Hoxa2表达水平导致不同的颅面异常和同源表型

IF 2.7

Journal of Developmental Biology Pub Date : 2022-01-31 DOI: 10.3390/jdb10010009

Taro Kitazawa, Maryline Minoux, S. Ducret, F. Rijli

{"title":"Different Ectopic Hoxa2 Expression Levels in Mouse Cranial Neural Crest Cells Result in Distinct Craniofacial Anomalies and Homeotic Phenotypes","authors":"Taro Kitazawa, Maryline Minoux, S. Ducret, F. Rijli","doi":"10.3390/jdb10010009","DOIUrl":"https://doi.org/10.3390/jdb10010009","url":null,"abstract":"Providing appropriate positional identity and patterning information to distinct rostrocaudal subpopulations of cranial neural crest cells (CNCCs) is central to vertebrate craniofacial morphogenesis. Hox genes are not expressed in frontonasal and first pharyngeal arch (PA1) CNCCs, whereas a single Hox gene, Hoxa2, is necessary to provide patterning information to second pharyngeal arch (PA2) CNCCs. In frog, chick and mouse embryos, ectopic expression of Hoxa2 in Hox-negative CNCCs induced hypoplastic phenotypes of CNCC derivatives of variable severity, associated or not with homeotic transformation of a subset of PA1 structures into a PA2-like identity. Whether these different morphological outcomes are directly related to distinct Hoxa2 overexpression levels is unknown. To address this issue, we selectively induced Hoxa2 overexpression in mouse CNCCs, using a panel of mouse lines expressing different Hoxa2 ectopic expression levels, including a newly generated Hoxa2 knocked-in mouse line. While ectopic Hoxa2 expression at only 60% of its physiological levels was sufficient for pinna duplication, ectopic Hoxa2 expression at 100% of its normal level was required for complete homeotic repatterning of a subset of PA1 skeletal elements into a duplicated set of PA2-like elements. On the other hand, ectopic Hoxa2 overexpression at non-physiological levels (200% of normal levels) led to an almost complete loss of craniofacial skeletal structures. Moreover, ectopic Hoxa5 overexpression in CNCCs, while also resulting in severe craniofacial defects, did not induce homeotic changes of PA1-derived CNCCs, indicating Hoxa2 specificity in repatterning a subset of Hox-negative CNCCs. These results reconcile some discrepancies in previously published experiments and indicate that distinct subpopulations of CNCCs are differentially sensitive to ectopic levels of Hox expression.","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2022-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45633766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Acknowledgment to Reviewers of Journal of Developmental Biology in 2021 感谢《发育生物学杂志》2021年审稿人

IF 2.7

Journal of Developmental Biology Pub Date : 2022-01-28 DOI: 10.3390/jdb10010008

引用次数: 0

Expression and Function of Toll Pathway Components in the Early Development of the Wasp Nasonia vitripennis 玻璃砂蜂早期发育过程中Toll通路组分的表达和功能

IF 2.7

Journal of Developmental Biology Pub Date : 2022-01-26 DOI: 10.3390/jdb10010007

D. Pers, Thomas Buchta, Orhan Özüak, S. Roth, Jeremy A. Lynch

引用次数: 1

Differentiation of Cells Isolated from Human Femoral Heads into Functional Osteoclasts 从人股骨头分离的细胞分化为功能性破骨细胞

IF 2.7

Journal of Developmental Biology Pub Date : 2022-01-18 DOI: 10.3390/jdb10010006

Daniel R Halloran, Brian P. Heubel, Connor MacMurray, D. Root, M. Eskander, Sean McTague, Heather Pelkey, A. Nohe

{"title":"Differentiation of Cells Isolated from Human Femoral Heads into Functional Osteoclasts","authors":"Daniel R Halloran, Brian P. Heubel, Connor MacMurray, D. Root, M. Eskander, Sean McTague, Heather Pelkey, A. Nohe","doi":"10.3390/jdb10010006","DOIUrl":"https://doi.org/10.3390/jdb10010006","url":null,"abstract":"Proper formation of the skeleton during development is crucial for the mobility of humans and the maintenance of essential organs. The production of bone is regulated by osteoblasts and osteoclasts. An imbalance of these cells can lead to a decrease in bone mineral density, which leads to fractures. While many studies are emerging to understand the role of osteoblasts, less studies are present about the role of osteoclasts. This present study utilized bone marrow cells isolated directly from the bone marrow of femoral heads obtained from osteoarthritic (OA) patients after undergoing hip replacement surgery. Here, we used tartrate resistant acid phosphatase (TRAP) staining, Cathepsin K, and nuclei to identity osteoclasts and their functionality after stimulation with macrophage-colony stimulation factor (M-CSF) and receptor activator of nuclear factor kappa-β ligand (RANKL). Our data demonstrated that isolated cells can be differentiated into functional osteoclasts, as indicated by the 92% and 83% of cells that stained positive for TRAP and Cathepsin K, respectively. Furthermore, isolated cells remain viable and terminally differentiate into osteoclasts when stimulated with RANKL. These data demonstrate that cells isolated from human femoral heads can be differentiated into osteoclasts to study bone disorders during development and adulthood.","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2022-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49384927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

The Immunoglobulin Superfamily Members syg-2 and syg-1 Regulate Neurite Development in C. elegans. 免疫球蛋白超家族成员syg-2和syg-1调节秀丽隐杆线虫的神经突发育。

IF 2.7

Journal of Developmental Biology Pub Date : 2022-01-09 DOI: 10.3390/jdb10010003

Dana K Tucker, Chloe S Adams, Gauri Prasad, Brian D Ackley

{"title":"The Immunoglobulin Superfamily Members syg-2 and syg-1 Regulate Neurite Development in C. elegans.","authors":"Dana K Tucker, Chloe S Adams, Gauri Prasad, Brian D Ackley","doi":"10.3390/jdb10010003","DOIUrl":"https://doi.org/10.3390/jdb10010003","url":null,"abstract":"Neurons form elaborate networks by guiding axons and dendrites to appropriate destinations. Neurites require information about the relative body axes during the initial projection from the cell body, and failure to receive or interpret those cues correctly can result in outgrowth errors. We identified a mutation in the Ig superfamily member syg-2 in a screen for animals with anterior/posterior (A/P) axon guidance defects. We found that syg-2 and its cognate Ig family member syg-1 appear to function in a linear genetic pathway to control the outgrowth of GABAergic axons. We determined that this pathway works in parallel to Wnt signaling. Specifically, mutations in syg-2 or syg-1 selectively affected the embryonically derived Dorsal D-type (DD) GABAergic neurons. We found no evidence that these mutations affected the Ventral D-type neurons (VD) that form later, during the first larval stage. In addition, mutations in syg-1 or syg-2 could result in the DD neurons forming multiple processes, becoming bipolar, rather than the expected pseudounipolar morphology. Given SYG-2's essential function in synaptogenesis of the hermaphrodite-specific neurons (HSNs), we also examined DD neuron synapses in syg-2 mutants. We found syg-2 mutants had a decreased number of synapses formed, but synaptic morphology was largely normal. These results provide further evidence that the GABAergic motorneurons use multiple guidance pathways during development.","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":"10 1","pages":""},"PeriodicalIF":2.7,"publicationDate":"2022-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8788504/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9575193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1