Journal of Developmental Biology最新文献_第9页

Coordination of Cilia Movements in Multi-Ciliated Cells. 多纤毛细胞中纤毛运动的协调。

IF 2.7

Journal of Developmental Biology Pub Date : 2022-11-11 DOI: 10.3390/jdb10040047

Masaki Arata, Fumiko Matsukawa Usami, Toshihiko Fujimori

引用次数: 1

Appropriate Amounts and Activity of the Wilms' Tumor Suppressor Gene, wt1, Are Required for Normal Pronephros Development of Xenopus Embryos. 爪蟾胚胎正常原肾发育需要适当数量和活性的Wilms肿瘤抑制基因wt1。

IF 2.7

Journal of Developmental Biology Pub Date : 2022-10-29 DOI: 10.3390/jdb10040046

Taisei Shiraki, Takuma Hayashi, Jotaro Ozue, Minoru Watanabe

{"title":"Appropriate Amounts and Activity of the Wilms' Tumor Suppressor Gene, wt1, Are Required for Normal Pronephros Development of Xenopus Embryos.","authors":"Taisei Shiraki, Takuma Hayashi, Jotaro Ozue, Minoru Watanabe","doi":"10.3390/jdb10040046","DOIUrl":"https://doi.org/10.3390/jdb10040046","url":null,"abstract":"The Wilms' tumor suppressor gene, wt1, encodes a zinc finger-containing transcription factor that binds to a GC-rich motif and regulates the transcription of target genes. wt1 was first identified as a tumor suppressor gene in Wilms' tumor, a pediatric kidney tumor, and has been implicated in normal kidney development. The WT1 protein has transcriptional activation and repression domains and acts as a transcriptional activator or repressor, depending on the target gene and context. In Xenopus, an ortholog of wt1 has been isolated and shown to be expressed in the developing embryonic pronephros. To investigate the role of wt1 in pronephros development in Xenopus embryos, we mutated wt1 by CRISPR/Cas9 and found that the expression of pronephros marker genes was reduced. In reporter assays in which known WT1 binding sequences were placed upstream of the luciferase gene, WT1 activated transcription of the luciferase gene. The injection of wild-type or artificially altered transcriptional activity of wt1 mRNA disrupted the expression of pronephros marker genes in the embryos. These results suggest that the appropriate amounts and activity of WT1 protein are required for normal pronephros development in Xenopus embryos.","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2022-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9680428/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10383228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Involvement of a Basic Helix-Loop-Helix Gene BHLHE40 in Specification of Chicken Retinal Pigment Epithelium. 碱性螺旋-环-螺旋基因BHLHE40参与鸡视网膜色素上皮的形成。

IF 2.7

Journal of Developmental Biology Pub Date : 2022-10-29 DOI: 10.3390/jdb10040045

Toshiki Kinuhata, Keita Sato, Tetsuya Bando, Taro Mito, Satoru Miyaishi, Tsutomu Nohno, Hideyo Ohuchi

{"title":"Involvement of a Basic Helix-Loop-Helix Gene BHLHE40 in Specification of Chicken Retinal Pigment Epithelium.","authors":"Toshiki Kinuhata, Keita Sato, Tetsuya Bando, Taro Mito, Satoru Miyaishi, Tsutomu Nohno, Hideyo Ohuchi","doi":"10.3390/jdb10040045","DOIUrl":"https://doi.org/10.3390/jdb10040045","url":null,"abstract":"The first event of differentiation and morphogenesis in the optic vesicle (OV) is specification of the neural retina (NR) and retinal pigment epithelium (RPE), separating the inner and outer layers of the optic cup, respectively. Here, we focus on a basic helix-loop-helix gene, BHLHE40, which has been shown to be expressed by the developing RPE in mice and zebrafish. Firstly, we examined the expression pattern of BHLHE40 in the developing chicken eye primordia by in situ hybridization. Secondly, BHLHE40 overexpression was performed with in ovo electroporation and its effects on optic cup morphology and expression of NR and RPE marker genes were examined. Thirdly, we examined the expression pattern of BHLHE40 in LHX1-overexpressed optic cup. BHLHE40 expression emerged in a subset of cells of the OV at Hamburger and Hamilton stage 14 and became confined to the outer layer of the OV and the ciliary marginal zone of the retina by stage 17. BHLHE40 overexpression in the prospective NR resulted in ectopic induction of OTX2 and repression of VSX2. Conversely, BHLHE40 was repressed in the second NR after LHX1 overexpression. These results suggest that emergence of BHLHE40 expression in the OV is involved in initial RPE specification and that BHLHE40 plays a role in separation of the early OV domains by maintaining OTX2 expression and antagonizing an NR developmental program.","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2022-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9680343/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10672974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Quantitative Experimental Embryology: A Modern Classical Approach. 定量实验胚胎学：现代经典方法》。

IF 2.2

Journal of Developmental Biology Pub Date : 2022-10-18 DOI: 10.3390/jdb10040044

Lara Busby, Dillan Saunders, Guillermo Serrano Nájera, Benjamin Steventon

引用次数: 0

Germ Granules in Animal Oogenesis. 动物卵发生中的胚芽颗粒。

IF 2.7

Journal of Developmental Biology Pub Date : 2022-10-09 DOI: 10.3390/jdb10040043

Mikhail A Dobrynin, Ekaterina O Bashendjieva, Natella I Enukashvily

引用次数: 2

Zebrafish Model of Stickler Syndrome Suggests a Role for Col2a1a in the Neural Crest during Early Eye Development. 斑马鱼Stickler综合征模型提示Col2a1a在早期眼睛发育过程中的神经嵴中的作用

IF 2.7

Journal of Developmental Biology Pub Date : 2022-10-01 DOI: 10.3390/jdb10040042

Antionette L Williams, Brenda L Bohnsack

{"title":"Zebrafish Model of Stickler Syndrome Suggests a Role for Col2a1a in the Neural Crest during Early Eye Development.","authors":"Antionette L Williams, Brenda L Bohnsack","doi":"10.3390/jdb10040042","DOIUrl":"https://doi.org/10.3390/jdb10040042","url":null,"abstract":"Most cases of Stickler syndrome are due to autosomal-dominant COL2A1 gene mutations leading to abnormal type II collagen. Ocular findings include axial eye lengthening with vitreal degeneration and early-onset glaucoma, which can result in vision loss. Although COL2A1 is a major player in cartilage and bone formation, its specific role in eye development remains elusive. We investigated the role of Col2a1a in neural crest migration and differentiation during early zebrafish eye development. In situ hybridization, immunofluorescence, live imaging, exogenous treatments [10 μM diethylaminobenzaldehyde (DEAB), 100 nM all-trans retinoic acid (RA) and 1-3% ethanol (ETOH)] and morpholino oligonucleotide (MO) injections were used to analyze wildtype Casper (roy-/-;nacre-/-), TgBAC(col2a1a::EGFP), Tg(sox10::EGFP) and Tg(foxd3::EGFP) embryos. Col2a1a colocalized with Foxd3- and Sox10-positive cells in the anterior segment and neural crest-derived jaw. Col2a1a expression was regulated by RA and inhibited by 3% ETOH. Furthermore, MO knockdown of Col2a1a delayed jaw formation and disrupted the ocular anterior segment neural crest migration of Sox10-positive cells. Interestingly, human COL2A1 protein rescued the MO effects. Altogether, these results suggest that Col2a1a is a downstream target of RA in the cranial neural crest and is required for both craniofacial and eye development.","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9589970/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40583980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Zebrafish Slit2 and Slit3 Act Together to Regulate Retinal Axon Crossing at the Midline. 斑马鱼Slit2和Slit3共同作用调节视网膜轴突中线交叉。

IF 2.7

Journal of Developmental Biology Pub Date : 2022-09-23 DOI: 10.3390/jdb10040041

Camila Davison, Gabriela Bedó, Flavio R Zolessi

{"title":"Zebrafish Slit2 and Slit3 Act Together to Regulate Retinal Axon Crossing at the Midline.","authors":"Camila Davison, Gabriela Bedó, Flavio R Zolessi","doi":"10.3390/jdb10040041","DOIUrl":"https://doi.org/10.3390/jdb10040041","url":null,"abstract":"Slit-Robo signaling regulates midline crossing of commissural axons in different systems. In zebrafish, all retinofugal axons cross at the optic chiasm to innervate the contralateral tectum. Here, the mutant for the Robo2 receptor presents severe axon guidance defects, which were not completely reproduced in a Slit2 ligand null mutant. Since slit3 is also expressed around this area at the stage of axon crossing, we decided to analyze the possibility that it collaborates with Slit2 in this process. We found that the disruption of slit3 expression by sgRNA-Cas9 injection caused similar, albeit slightly milder, defects than those of the slit2 mutant, while the same treatment in the slit2-/-mz background caused much more severe defects, comparable to those observed in robo2 mutants. Tracking analysis of in vivo time-lapse experiments indicated differential but complementary functions of these secreted factors in the correction of axon turn errors around the optic chiasm. Interestingly, RT-qPCR analysis showed a mild increase in slit2 expression in slit3-deficient embryos, but not the opposite. Our observations support the previously proposed \"repulsive channel\" model for Slit-Robo action at the optic chiasm, with both Slits acting in different manners, most probably relating to their different spatial expression patterns.","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2022-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9590056/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40583979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Shape of the Jaw-Zebrafish Col11a1a Regulates Meckel's Cartilage Morphogenesis and Mineralization. 颌-斑马鱼Col11a1a的形状调节梅克尔软骨的形态发生和矿化。

IF 2.7

Journal of Developmental Biology Pub Date : 2022-09-22 DOI: 10.3390/jdb10040040

Jonathon C Reeck, Julia Thom Oxford

{"title":"The Shape of the Jaw-Zebrafish Col11a1a Regulates Meckel's Cartilage Morphogenesis and Mineralization.","authors":"Jonathon C Reeck, Julia Thom Oxford","doi":"10.3390/jdb10040040","DOIUrl":"https://doi.org/10.3390/jdb10040040","url":null,"abstract":"The expression of the col11a1a gene is essential for normal skeletal development, affecting both cartilage and bone. Loss of function mutations have been shown to cause abnormalities in the growth plate of long bones, as well as in craniofacial development. However, the specific effects on Meckel's cartilage have not been well studied. To further understand the effect of col11a1a gene function, we analyzed the developing jaw in zebrafish using gene knockdown by the injection of an antisense morpholino oligonucleotide using transgenic Tg(sp7:EGFP) and Tg(Fli1a:EGFP) EGFP reporter fish, as well as wildtype AB zebrafish. Our results demonstrate that zebrafish col11a1a knockdown impairs the cellular organization of Meckel's cartilage in the developing jaw and alters the bone formation that occurs adjacent to the Meckel's cartilage. These results suggest roles for Col11a1a protein in cartilage intermediates of bone development, the subsequent mineralization of the bony collar of long bones, and that which occurs adjacent to Meckel's cartilage in the developing jaw.","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2022-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9590009/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10723300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Extracellular Vesicles and Membrane Protrusions in Developmental Signaling. 发育信号中的细胞外小泡和膜突起

IF 2.2

Journal of Developmental Biology Pub Date : 2022-09-21 DOI: 10.3390/jdb10040039

Callie M Gustafson, Laura S Gammill

引用次数: 0

Tissue Rotation of the Xenopus Anterior-Posterior Neural Axis Reveals Profound but Transient Plasticity at the Mid-Gastrula Stage. 非洲爪蟾前后神经轴的组织旋转显示出在中胃泌素阶段的深刻但短暂的可塑性。

IF 2.2

Journal of Developmental Biology Pub Date : 2022-09-10 DOI: 10.3390/jdb10030038

Lyuba Bolkhovitinov, Bryan T Weselman, Gladys A Shaw, Chen Dong, Janhavi Giribhattanavar, Margaret S Saha

{"title":"Tissue Rotation of the Xenopus Anterior-Posterior Neural Axis Reveals Profound but Transient Plasticity at the Mid-Gastrula Stage.","authors":"Lyuba Bolkhovitinov, Bryan T Weselman, Gladys A Shaw, Chen Dong, Janhavi Giribhattanavar, Margaret S Saha","doi":"10.3390/jdb10030038","DOIUrl":"10.3390/jdb10030038","url":null,"abstract":"The establishment of anterior-posterior (AP) regional identity is an essential step in the appropriate development of the vertebrate central nervous system. An important aspect of AP neural axis formation is the inherent plasticity that allows developing cells to respond to and recover from the various perturbations that embryos continually face during the course of development. While the mechanisms governing the regionalization of the nervous system have been extensively studied, relatively less is known about the nature and limits of early neural plasticity of the anterior-posterior neural axis. This study aims to characterize the degree of neural axis plasticity in Xenopus laevis by investigating the response of embryos to a 180-degree rotation of their AP neural axis during gastrula stages by assessing the expression of regional marker genes using in situ hybridization. Our results reveal the presence of a narrow window of time between the mid- and late gastrula stage, during which embryos are able undergo significant recovery following a 180-degree rotation of their neural axis and eventually express appropriate regional marker genes including Otx, Engrailed, and Krox. By the late gastrula stage, embryos show misregulation of regional marker genes following neural axis rotation, suggesting that this profound axial plasticity is a transient phenomenon that is lost by late gastrula stages.","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2022-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9503425/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9194361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0