Journal of Developmental Biology最新文献_第8页

Scientific Papers by Developmental Biologists in Japan. 日本发育生物学家的科学论文。

IF 2.7

Journal of Developmental Biology Pub Date : 2023-03-10 DOI: 10.3390/jdb11010011

Hideyo Ohuchi, Tsutomu Nohno

引用次数: 0

In Vitro Comparison of Sex-Specific Splicing Efficiencies of fem Pre-mRNA under Monoallelic and Heteroallelic Conditions of csd, a Master Sex-Determining Gene in the Honeybee. 蜜蜂主要性别决定基因csd单等位基因和异等位基因条件下fem Pre-mRNA体外性别特异性剪接效率的比较

IF 2.7

Journal of Developmental Biology Pub Date : 2023-03-10 DOI: 10.3390/jdb11010010

Yukihiro Suzuki, Takafumi Yamada, Masataka G Suzuki

{"title":"In Vitro Comparison of Sex-Specific Splicing Efficiencies of fem Pre-mRNA under Monoallelic and Heteroallelic Conditions of csd, a Master Sex-Determining Gene in the Honeybee.","authors":"Yukihiro Suzuki, Takafumi Yamada, Masataka G Suzuki","doi":"10.3390/jdb11010010","DOIUrl":"https://doi.org/10.3390/jdb11010010","url":null,"abstract":"The sexual fate of honeybees is determined by the complementary sex determination (CSD) model: heterozygosity at a single locus (the CSD locus) determines femaleness, while hemizygosity or homozygosity at the CSD locus determines maleness. The csd gene encodes a splicing factor that regulates sex-specific splicing of the downstream target gene feminizer (fem), which is required for femaleness. The female mode of fem splicing occurs only when csd is present in the heteroallelic condition. To gain insights into how Csd proteins are only activated under the heterozygous allelic composition, we developed an in vitro assay system to evaluate the activity of Csd proteins. Consistent with the CSD model, the co-expression of two csd alleles, both of which lack splicing activity under the single-allele condition, restored the splicing activity that governs the female mode of fem splicing. RNA immunoprecipitation quantitative PCR analyses demonstrated that the CSD protein was specifically enriched in several exonic regions in the fem pre-mRNA, and enrichment in exons 3a and 5 was significantly greater under the heterozygous allelic composition than the single-allelic condition. However, in most cases csd expression under the monoallelic condition was capable of inducing the female mode of fem splicing contrary to the conventional CSD model. In contrast, repression of the male mode of fem splicing was predominant under heteroallelic conditions. These results were reproduced by real-time PCR of endogenous fem expression in female and male pupae. These findings strongly suggest that the heteroallelic composition of csd may be more important for the repression of the male splicing mode than for the induction of the female splicing mode of the fem gene.","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":"11 1","pages":""},"PeriodicalIF":2.7,"publicationDate":"2023-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10057164/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9204494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modeling Podocyte Ontogeny and Podocytopathies with the Zebrafish. 用斑马鱼模拟荚膜细胞的本体发育和荚膜病变

IF 2.2

Journal of Developmental Biology Pub Date : 2023-02-20 DOI: 10.3390/jdb11010009

Bridgette E Drummond, Wesley S Ercanbrack, Rebecca A Wingert

引用次数: 0

Neurogenin 2 and Neuronal Differentiation 1 Control Proper Development of the Chick Trigeminal Ganglion and Its Nerve Branches. 神经原素2和神经元分化1控制鸡三叉神经节及其神经分支的正常发育。

IF 2.7

Journal of Developmental Biology Pub Date : 2023-02-19 DOI: 10.3390/jdb11010008

Parinaz Bina, Margaret A Hines, Johena Sanyal, Lisa A Taneyhill

{"title":"Neurogenin 2 and Neuronal Differentiation 1 Control Proper Development of the Chick Trigeminal Ganglion and Its Nerve Branches.","authors":"Parinaz Bina, Margaret A Hines, Johena Sanyal, Lisa A Taneyhill","doi":"10.3390/jdb11010008","DOIUrl":"https://doi.org/10.3390/jdb11010008","url":null,"abstract":"The trigeminal ganglion contains the cell bodies of sensory neurons comprising cranial nerve V, which relays information related to pain, touch, and temperature from the face and head to the brain. Like other cranial ganglia, the trigeminal ganglion is composed of neuronal derivatives of two critical embryonic cell types, neural crest and placode cells. Neurogenesis within the cranial ganglia is promoted by Neurogenin 2 (Neurog2), which is expressed in trigeminal placode cells and their neuronal derivatives, and transcriptionally activates neuronal differentiation genes such as Neuronal Differentiation 1 (NeuroD1). Little is known, however, about the role of Neurog2 and NeuroD1 during chick trigeminal gangliogenesis. To address this, we depleted Neurog2 and NeuroD1 from trigeminal placode cells with morpholinos and demonstrated that Neurog2 and NeuroD1 influence trigeminal ganglion development. While knockdown of both Neurog2 and NeuroD1 affected innervation of the eye, Neurog2 and NeuroD1 had opposite effects on ophthalmic nerve branch organization. Taken together, our results highlight, for the first time, functional roles for Neurog2 and NeuroD1 during chick trigeminal gangliogenesis. These studies shed new light on the molecular mechanisms underlying trigeminal ganglion formation and may also provide insight into general cranial gangliogenesis and diseases of the peripheral nervous system.","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":"11 1","pages":""},"PeriodicalIF":2.7,"publicationDate":"2023-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9953625/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9233567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Introduction to the Development of Skin in Vertebrates. 脊椎动物皮肤发育概论。

IF 2.7

Journal of Developmental Biology Pub Date : 2023-01-31 DOI: 10.3390/jdb11010007

Lorenzo Alibardi

引用次数: 0

The Complex Bridge between Aquatic and Terrestrial Life: Skin Changes during Development of Amphibians. 水生生物与陆生生物之间的复杂桥梁：两栖动物发育过程中的皮肤变化

IF 2.2

Journal of Developmental Biology Pub Date : 2023-01-30 DOI: 10.3390/jdb11010006

Esra Akat Çömden, Melodi Yenmiş, Berna Çakır

引用次数: 0

The Story of the Finest Armor: Developmental Aspects of Reptile Skin. 最好的盔甲的故事:爬行动物皮肤的发育方面。

IF 2.7

Journal of Developmental Biology Pub Date : 2023-01-28 DOI: 10.3390/jdb11010005

Melodi Yenmiş, Dinçer Ayaz

{"title":"The Story of the Finest Armor: Developmental Aspects of Reptile Skin.","authors":"Melodi Yenmiş, Dinçer Ayaz","doi":"10.3390/jdb11010005","DOIUrl":"https://doi.org/10.3390/jdb11010005","url":null,"abstract":"The reptile skin is a barrier against water loss and pathogens and an armor for mechanical damages. The integument of reptiles consists of two main layers: the epidermis and the dermis. The epidermis, the hard cover of the body which has an armor-like role, varies among extant reptiles in terms of structural aspects such as thickness, hardness or the kinds of appendages it constitutes. The reptile epithelial cells of the epidermis (keratinocytes) are composed of two main proteins: intermediate filament keratins (IFKs) and corneous beta proteins (CBPs). The outer horny layer of the epidermis, stratum corneum, is constituted of keratinocytes by means of terminal differentiation or cornification which is a result of the protein interactions where CBPs associate with and coat the initial scaffold of IFKs. Reptiles were able to colonize the terrestrial environment due to the changes in these epidermal structures, which led to various cornified epidermal appendages such as scales and scutes, a beak, claws or setae. Developmental and structural aspects of the epidermal CBPs as well as their shared chromosomal locus (EDC) indicate an ancestral origin that gave rise to the finest armor of reptilians.","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":"11 1","pages":""},"PeriodicalIF":2.7,"publicationDate":"2023-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9944452/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9313037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Acknowledgment to the Reviewers of Journal of Developmental Biology in 2022. 感谢《发育生物学杂志》2022年审稿人。

IF 2.7

Journal of Developmental Biology Pub Date : 2023-01-17 DOI: 10.3390/jdb11010004

Jdb Editorial Office

引用次数: 0

The Periodic Replacement of Adhesive Setae in Pad Lamellae of Climbing Lizards Is Driven by Patterns of Corneous Layer Growth. 攀爬蜥蜴垫片粘附刚毛的周期性更替是由角质层生长模式驱动的。

IF 2.7

Journal of Developmental Biology Pub Date : 2022-12-30 DOI: 10.3390/jdb11010003

Lorenzo Alibardi

{"title":"The Periodic Replacement of Adhesive Setae in Pad Lamellae of Climbing Lizards Is Driven by Patterns of Corneous Layer Growth.","authors":"Lorenzo Alibardi","doi":"10.3390/jdb11010003","DOIUrl":"https://doi.org/10.3390/jdb11010003","url":null,"abstract":"The adhesive digital pads in some gecko and anoline lizards are continuously utilized for movements on vertical surfaces that may determine wear and a decrease of adhesion efficiency. The pads are formed by lamellae bearing adhesive setae that are worn out following frequent usage and are replaced by new inner setae that maintain an efficient adhesion. Whether the extensive usage of adhesive setae determines a higher shedding frequency in the digital pads with respect to other body regions remains unknown. Setae replacement has been analyzed in embryos and adult lizards using autoradiography and 5BrdU-immunohistochemistry. The observation strongly suggests that during development and epidermal renewal in adult lamellae, there is a shifting of the outer setae toward the apex of the lamella. This movement is likely derived from the continuous addition of proteins in the beta- and alpha-layers sustaining the outer setae while the inner setae are forming. Ultrastructural and in situ hybridization studies indicate that the thin outer beta- and alpha-layers still contain mRNAs and ribosomes that may contribute to the continuous production of corneous beta proteins (CBPs) and keratins for the growth of the free margin at the apex of the lamella. This process determines the apical shifting and release of the old setae, while the new inner setae formed underneath becomes the new outer setae.","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":"11 1","pages":""},"PeriodicalIF":2.7,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9844433/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9099763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Roles for the RNA-Binding Protein Caper in Reproductive Output in Drosophila melanogaster. rna结合蛋白Caper在果蝇生殖输出中的作用。

IF 2.7

Journal of Developmental Biology Pub Date : 2022-12-23 DOI: 10.3390/jdb11010002

Erika J Tixtha, Meg K Super, M Brandon Titus, Jeremy M Bono, Eugenia C Olesnicky

{"title":"Roles for the RNA-Binding Protein Caper in Reproductive Output in Drosophila melanogaster.","authors":"Erika J Tixtha, Meg K Super, M Brandon Titus, Jeremy M Bono, Eugenia C Olesnicky","doi":"10.3390/jdb11010002","DOIUrl":"https://doi.org/10.3390/jdb11010002","url":null,"abstract":"RNA binding proteins (RBPs) play a fundamental role in the post-transcriptional regulation of gene expression within the germline and nervous system. This is underscored by the prevalence of mutations within RBP-encoding genes being implicated in infertility and neurological disease. We previously described roles for the highly conserved RBP Caper in neurite morphogenesis in the Drosophila larval peripheral system and in locomotor behavior. However, caper function has not been investigated outside the nervous system, although it is widely expressed in many different tissue types during embryogenesis. Here, we describe novel roles for Caper in fertility and mating behavior. We find that Caper is expressed in ovarian follicles throughout oogenesis but is dispensable for proper patterning of the egg chamber. Additionally, reduced caper function, through either a genetic lesion or RNA interference-mediated knockdown of caper in the female germline, results in females laying significantly fewer eggs than their control counterparts. Moreover, this phenotype is exacerbated with age. caper dysfunction also results in partial embryonic and larval lethality. Given that caper is highly conserved across metazoa, these findings may also be relevant to vertebrates.","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":"11 1","pages":""},"PeriodicalIF":2.7,"publicationDate":"2022-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9844462/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9366862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0