Journal of Developmental Biology最新文献_第8页

Organophosphate Insecticide Toxicity in Neural Development, Cognition, Behaviour and Degeneration: Insights from Zebrafish. 有机磷杀虫剂对神经发育、认知、行为和退化的毒性:来自斑马鱼的见解。

IF 2.7

Journal of Developmental Biology Pub Date : 2022-11-21 DOI: 10.3390/jdb10040049

Jeremy Neylon, Jarrad N Fuller, Chris van der Poel, Jarrod E Church, Sebastian Dworkin

引用次数: 8

Seeking Sense in the Hox Gene Cluster. 在 Hox 基因簇中寻找意义

IF 2.2

Journal of Developmental Biology Pub Date : 2022-11-15 DOI: 10.3390/jdb10040048

Stephen J Gaunt

引用次数: 0

Coordination of Cilia Movements in Multi-Ciliated Cells. 多纤毛细胞中纤毛运动的协调。

IF 2.7

Journal of Developmental Biology Pub Date : 2022-11-11 DOI: 10.3390/jdb10040047

Masaki Arata, Fumiko Matsukawa Usami, Toshihiko Fujimori

引用次数: 1

Appropriate Amounts and Activity of the Wilms' Tumor Suppressor Gene, wt1, Are Required for Normal Pronephros Development of Xenopus Embryos. 爪蟾胚胎正常原肾发育需要适当数量和活性的Wilms肿瘤抑制基因wt1。

IF 2.7

Journal of Developmental Biology Pub Date : 2022-10-29 DOI: 10.3390/jdb10040046

Taisei Shiraki, Takuma Hayashi, Jotaro Ozue, Minoru Watanabe

{"title":"Appropriate Amounts and Activity of the Wilms' Tumor Suppressor Gene, wt1, Are Required for Normal Pronephros Development of Xenopus Embryos.","authors":"Taisei Shiraki, Takuma Hayashi, Jotaro Ozue, Minoru Watanabe","doi":"10.3390/jdb10040046","DOIUrl":"https://doi.org/10.3390/jdb10040046","url":null,"abstract":"The Wilms' tumor suppressor gene, wt1, encodes a zinc finger-containing transcription factor that binds to a GC-rich motif and regulates the transcription of target genes. wt1 was first identified as a tumor suppressor gene in Wilms' tumor, a pediatric kidney tumor, and has been implicated in normal kidney development. The WT1 protein has transcriptional activation and repression domains and acts as a transcriptional activator or repressor, depending on the target gene and context. In Xenopus, an ortholog of wt1 has been isolated and shown to be expressed in the developing embryonic pronephros. To investigate the role of wt1 in pronephros development in Xenopus embryos, we mutated wt1 by CRISPR/Cas9 and found that the expression of pronephros marker genes was reduced. In reporter assays in which known WT1 binding sequences were placed upstream of the luciferase gene, WT1 activated transcription of the luciferase gene. The injection of wild-type or artificially altered transcriptional activity of wt1 mRNA disrupted the expression of pronephros marker genes in the embryos. These results suggest that the appropriate amounts and activity of WT1 protein are required for normal pronephros development in Xenopus embryos.","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":"10 4","pages":""},"PeriodicalIF":2.7,"publicationDate":"2022-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9680428/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10383228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Involvement of a Basic Helix-Loop-Helix Gene BHLHE40 in Specification of Chicken Retinal Pigment Epithelium. 碱性螺旋-环-螺旋基因BHLHE40参与鸡视网膜色素上皮的形成。

IF 2.7

Journal of Developmental Biology Pub Date : 2022-10-29 DOI: 10.3390/jdb10040045

Toshiki Kinuhata, Keita Sato, Tetsuya Bando, Taro Mito, Satoru Miyaishi, Tsutomu Nohno, Hideyo Ohuchi

{"title":"Involvement of a Basic Helix-Loop-Helix Gene BHLHE40 in Specification of Chicken Retinal Pigment Epithelium.","authors":"Toshiki Kinuhata, Keita Sato, Tetsuya Bando, Taro Mito, Satoru Miyaishi, Tsutomu Nohno, Hideyo Ohuchi","doi":"10.3390/jdb10040045","DOIUrl":"https://doi.org/10.3390/jdb10040045","url":null,"abstract":"The first event of differentiation and morphogenesis in the optic vesicle (OV) is specification of the neural retina (NR) and retinal pigment epithelium (RPE), separating the inner and outer layers of the optic cup, respectively. Here, we focus on a basic helix-loop-helix gene, BHLHE40, which has been shown to be expressed by the developing RPE in mice and zebrafish. Firstly, we examined the expression pattern of BHLHE40 in the developing chicken eye primordia by in situ hybridization. Secondly, BHLHE40 overexpression was performed with in ovo electroporation and its effects on optic cup morphology and expression of NR and RPE marker genes were examined. Thirdly, we examined the expression pattern of BHLHE40 in LHX1-overexpressed optic cup. BHLHE40 expression emerged in a subset of cells of the OV at Hamburger and Hamilton stage 14 and became confined to the outer layer of the OV and the ciliary marginal zone of the retina by stage 17. BHLHE40 overexpression in the prospective NR resulted in ectopic induction of OTX2 and repression of VSX2. Conversely, BHLHE40 was repressed in the second NR after LHX1 overexpression. These results suggest that emergence of BHLHE40 expression in the OV is involved in initial RPE specification and that BHLHE40 plays a role in separation of the early OV domains by maintaining OTX2 expression and antagonizing an NR developmental program.","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":"10 4","pages":""},"PeriodicalIF":2.7,"publicationDate":"2022-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9680343/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10672974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The Shape of the Jaw-Zebrafish Col11a1a Regulates Meckel's Cartilage Morphogenesis and Mineralization. 颌-斑马鱼Col11a1a的形状调节梅克尔软骨的形态发生和矿化。

IF 2.7

Journal of Developmental Biology Pub Date : 2022-09-22 DOI: 10.3390/jdb10040040

Jonathon C Reeck, Julia Thom Oxford

{"title":"The Shape of the Jaw-Zebrafish Col11a1a Regulates Meckel's Cartilage Morphogenesis and Mineralization.","authors":"Jonathon C Reeck, Julia Thom Oxford","doi":"10.3390/jdb10040040","DOIUrl":"https://doi.org/10.3390/jdb10040040","url":null,"abstract":"The expression of the col11a1a gene is essential for normal skeletal development, affecting both cartilage and bone. Loss of function mutations have been shown to cause abnormalities in the growth plate of long bones, as well as in craniofacial development. However, the specific effects on Meckel's cartilage have not been well studied. To further understand the effect of col11a1a gene function, we analyzed the developing jaw in zebrafish using gene knockdown by the injection of an antisense morpholino oligonucleotide using transgenic Tg(sp7:EGFP) and Tg(Fli1a:EGFP) EGFP reporter fish, as well as wildtype AB zebrafish. Our results demonstrate that zebrafish col11a1a knockdown impairs the cellular organization of Meckel's cartilage in the developing jaw and alters the bone formation that occurs adjacent to the Meckel's cartilage. These results suggest roles for Col11a1a protein in cartilage intermediates of bone development, the subsequent mineralization of the bony collar of long bones, and that which occurs adjacent to Meckel's cartilage in the developing jaw.","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":"10 4","pages":""},"PeriodicalIF":2.7,"publicationDate":"2022-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9590009/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10723300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Extracellular Vesicles and Membrane Protrusions in Developmental Signaling. 发育信号中的细胞外小泡和膜突起

IF 2.2

Journal of Developmental Biology Pub Date : 2022-09-21 DOI: 10.3390/jdb10040039

Callie M Gustafson, Laura S Gammill

引用次数: 0

Tissue Rotation of the Xenopus Anterior-Posterior Neural Axis Reveals Profound but Transient Plasticity at the Mid-Gastrula Stage. 非洲爪蟾前后神经轴的组织旋转显示出在中胃泌素阶段的深刻但短暂的可塑性。

IF 2.2

Journal of Developmental Biology Pub Date : 2022-09-10 DOI: 10.3390/jdb10030038

Lyuba Bolkhovitinov, Bryan T Weselman, Gladys A Shaw, Chen Dong, Janhavi Giribhattanavar, Margaret S Saha

{"title":"Tissue Rotation of the Xenopus Anterior-Posterior Neural Axis Reveals Profound but Transient Plasticity at the Mid-Gastrula Stage.","authors":"Lyuba Bolkhovitinov, Bryan T Weselman, Gladys A Shaw, Chen Dong, Janhavi Giribhattanavar, Margaret S Saha","doi":"10.3390/jdb10030038","DOIUrl":"10.3390/jdb10030038","url":null,"abstract":"The establishment of anterior-posterior (AP) regional identity is an essential step in the appropriate development of the vertebrate central nervous system. An important aspect of AP neural axis formation is the inherent plasticity that allows developing cells to respond to and recover from the various perturbations that embryos continually face during the course of development. While the mechanisms governing the regionalization of the nervous system have been extensively studied, relatively less is known about the nature and limits of early neural plasticity of the anterior-posterior neural axis. This study aims to characterize the degree of neural axis plasticity in Xenopus laevis by investigating the response of embryos to a 180-degree rotation of their AP neural axis during gastrula stages by assessing the expression of regional marker genes using in situ hybridization. Our results reveal the presence of a narrow window of time between the mid- and late gastrula stage, during which embryos are able undergo significant recovery following a 180-degree rotation of their neural axis and eventually express appropriate regional marker genes including Otx, Engrailed, and Krox. By the late gastrula stage, embryos show misregulation of regional marker genes following neural axis rotation, suggesting that this profound axial plasticity is a transient phenomenon that is lost by late gastrula stages.","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":"10 3","pages":""},"PeriodicalIF":2.2,"publicationDate":"2022-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9503425/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9194361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spina Bifida: A Review of the Genetics, Pathophysiology and Emerging Cellular Therapies. 脊柱裂:遗传学、病理生理学和新兴细胞疗法的综述。

IF 2.7

Journal of Developmental Biology Pub Date : 2022-06-06 DOI: 10.3390/jdb10020022

Abd-Elrahman Said Hassan, Yimeng Lina Du, Su Yeon Lee, Aijun Wang, Diana Lee Farmer

引用次数: 7

A Review of Delayed Delivery Models and the Analysis Method in Mice 小鼠延迟分娩模型及分析方法综述

IF 2.7

Journal of Developmental Biology Pub Date : 2022-05-20 DOI: 10.3390/jdb10020020

Hiroshi Yomogita, N. Miyasaka, M. Kanai-Azuma

{"title":"A Review of Delayed Delivery Models and the Analysis Method in Mice","authors":"Hiroshi Yomogita, N. Miyasaka, M. Kanai-Azuma","doi":"10.3390/jdb10020020","DOIUrl":"https://doi.org/10.3390/jdb10020020","url":null,"abstract":"In humans, the incidence of post-term delivery is 1–10%. Post-term delivery significantly increases the risk of cesarean section or neonatal intensive care unit (NICU) admission. Despite these serious challenges, the cause of prolonged delivery remains unclear. Several common factors of delayed parturition between mice and humans will help elucidate the mechanisms of pregnancy and labor. At present, gene modification techniques are rapidly developing; however, there are limited reviews available describing the mouse phenotype analysis as a human model for post-term delivery. We classified the delayed-labor mice into nine types according to their causes. In mice, progesterone (P₄) maintains pregnancy, and the most common cause of delayed labor is luteolysis failure. Other contributing factors include humoral molecules in the fetus/placenta, uterine contractile dysfunction, poor cervical ripening, and delayed implantation. The etiology of delayed parturition is overexpression of the pregnancy maintenance mechanism or suppression of the labor induction mechanism. Here, we describe how to investigated their causes using mouse genetic analysis. In addition, we generated a list to identify the causes. Our review will help understand the findings obtained using the mouse model, providing a foundation for conducting more systematic research on delayed delivery.","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2022-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41460287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2