Journal of Developmental Biology最新文献

{"title":"Neurogenin 2 and Neuronal Differentiation 1 Control Proper Development of the Chick Trigeminal Ganglion and Its Nerve Branches.","authors":"Parinaz Bina,&nbsp;Margaret A Hines,&nbsp;Johena Sanyal,&nbsp;Lisa A Taneyhill","doi":"10.3390/jdb11010008","DOIUrl":"https://doi.org/10.3390/jdb11010008","url":null,"abstract":"<p><p>The trigeminal ganglion contains the cell bodies of sensory neurons comprising cranial nerve V, which relays information related to pain, touch, and temperature from the face and head to the brain. Like other cranial ganglia, the trigeminal ganglion is composed of neuronal derivatives of two critical embryonic cell types, neural crest and placode cells. Neurogenesis within the cranial ganglia is promoted by Neurogenin 2 (Neurog2), which is expressed in trigeminal placode cells and their neuronal derivatives, and transcriptionally activates neuronal differentiation genes such as <i>Neuronal Differentiation 1</i> (<i>NeuroD1</i>). Little is known, however, about the role of Neurog2 and NeuroD1 during chick trigeminal gangliogenesis. To address this, we depleted Neurog2 and NeuroD1 from trigeminal placode cells with morpholinos and demonstrated that Neurog2 and NeuroD1 influence trigeminal ganglion development. While knockdown of both Neurog2 and NeuroD1 affected innervation of the eye, Neurog2 and NeuroD1 had opposite effects on ophthalmic nerve branch organization. Taken together, our results highlight, for the first time, functional roles for Neurog2 and NeuroD1 during chick trigeminal gangliogenesis. These studies shed new light on the molecular mechanisms underlying trigeminal ganglion formation and may also provide insight into general cranial gangliogenesis and diseases of the peripheral nervous system.</p>","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":"11 1","pages":""},"PeriodicalIF":2.7,"publicationDate":"2023-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9953625/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9233567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Introduction to the Development of Skin in Vertebrates.","authors":"Lorenzo Alibardi","doi":"10.3390/jdb11010007","DOIUrl":"https://doi.org/10.3390/jdb11010007","url":null,"abstract":"<p><p>The integument of vertebrates is a complex and large organ positioned at the interface with the aquatic or terrestrial environment, and is derived from the embryonic ectoderm (epidermis) and mesoderm (dermis and hypodermis) [...].</p>","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":"11 1","pages":""},"PeriodicalIF":2.7,"publicationDate":"2023-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9944064/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9313040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Complex Bridge between Aquatic and Terrestrial Life: Skin Changes during Development of Amphibians.","authors":"Esra Akat Çömden, Melodi Yenmiş, Berna Çakır","doi":"10.3390/jdb11010006","DOIUrl":"10.3390/jdb11010006","url":null,"abstract":"<p><p>Amphibian skin is a particularly complex organ that is primarily responsible for respiration, osmoregulation, thermoregulation, defense, water absorption, and communication. The skin, as well as many other organs in the amphibian body, has undergone the most extensive rearrangement in the adaptation from water to land. Structural and physiological features of skin in amphibians are presented within this review. We aim to procure extensive and updated information on the evolutionary history of amphibians and their transition from water to land-that is, the changes seen in their skin from the larval stages to adulthood from the points of morphology, physiology, and immunology.</p>","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":"11 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2023-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9944868/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9313038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Story of the Finest Armor: Developmental Aspects of Reptile Skin.","authors":"Melodi Yenmiş,&nbsp;Dinçer Ayaz","doi":"10.3390/jdb11010005","DOIUrl":"https://doi.org/10.3390/jdb11010005","url":null,"abstract":"<p><p>The reptile skin is a barrier against water loss and pathogens and an armor for mechanical damages. The integument of reptiles consists of two main layers: the epidermis and the dermis. The epidermis, the hard cover of the body which has an armor-like role, varies among extant reptiles in terms of structural aspects such as thickness, hardness or the kinds of appendages it constitutes. The reptile epithelial cells of the epidermis (keratinocytes) are composed of two main proteins: intermediate filament keratins (IFKs) and corneous beta proteins (CBPs). The outer horny layer of the epidermis, stratum corneum, is constituted of keratinocytes by means of terminal differentiation or cornification which is a result of the protein interactions where CBPs associate with and coat the initial scaffold of IFKs. Reptiles were able to colonize the terrestrial environment due to the changes in these epidermal structures, which led to various cornified epidermal appendages such as scales and scutes, a beak, claws or setae. Developmental and structural aspects of the epidermal CBPs as well as their shared chromosomal locus (EDC) indicate an ancestral origin that gave rise to the finest armor of reptilians.</p>","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":"11 1","pages":""},"PeriodicalIF":2.7,"publicationDate":"2023-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9944452/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9313037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acknowledgment to the Reviewers of <i>Journal of Developmental Biology</i> in 2022.","authors":"Jdb Editorial Office","doi":"10.3390/jdb11010004","DOIUrl":"https://doi.org/10.3390/jdb11010004","url":null,"abstract":"<p><p>High-quality academic publishing is built on rigorous peer review [...].</p>","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":"11 1","pages":""},"PeriodicalIF":2.7,"publicationDate":"2023-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9944099/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9327267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Periodic Replacement of Adhesive Setae in Pad Lamellae of Climbing Lizards Is Driven by Patterns of Corneous Layer Growth.","authors":"Lorenzo Alibardi","doi":"10.3390/jdb11010003","DOIUrl":"https://doi.org/10.3390/jdb11010003","url":null,"abstract":"<p><p>The adhesive digital pads in some gecko and anoline lizards are continuously utilized for movements on vertical surfaces that may determine wear and a decrease of adhesion efficiency. The pads are formed by lamellae bearing adhesive setae that are worn out following frequent usage and are replaced by new inner setae that maintain an efficient adhesion. Whether the extensive usage of adhesive setae determines a higher shedding frequency in the digital pads with respect to other body regions remains unknown. Setae replacement has been analyzed in embryos and adult lizards using autoradiography and 5BrdU-immunohistochemistry. The observation strongly suggests that during development and epidermal renewal in adult lamellae, there is a shifting of the outer setae toward the apex of the lamella. This movement is likely derived from the continuous addition of proteins in the beta- and alpha-layers sustaining the outer setae while the inner setae are forming. Ultrastructural and in situ hybridization studies indicate that the thin outer beta- and alpha-layers still contain mRNAs and ribosomes that may contribute to the continuous production of corneous beta proteins (CBPs) and keratins for the growth of the free margin at the apex of the lamella. This process determines the apical shifting and release of the old setae, while the new inner setae formed underneath becomes the new outer setae.</p>","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":"11 1","pages":""},"PeriodicalIF":2.7,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9844433/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9099763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Roles for the RNA-Binding Protein Caper in Reproductive Output in <i>Drosophila melanogaster</i>.","authors":"Erika J Tixtha,&nbsp;Meg K Super,&nbsp;M Brandon Titus,&nbsp;Jeremy M Bono,&nbsp;Eugenia C Olesnicky","doi":"10.3390/jdb11010002","DOIUrl":"https://doi.org/10.3390/jdb11010002","url":null,"abstract":"<p><p>RNA binding proteins (RBPs) play a fundamental role in the post-transcriptional regulation of gene expression within the germline and nervous system. This is underscored by the prevalence of mutations within RBP-encoding genes being implicated in infertility and neurological disease. We previously described roles for the highly conserved RBP Caper in neurite morphogenesis in the <i>Drosophila</i> larval peripheral system and in locomotor behavior. However, <i>caper</i> function has not been investigated outside the nervous system, although it is widely expressed in many different tissue types during embryogenesis. Here, we describe novel roles for Caper in fertility and mating behavior. We find that Caper is expressed in ovarian follicles throughout oogenesis but is dispensable for proper patterning of the egg chamber. Additionally, reduced <i>caper</i> function, through either a genetic lesion or RNA interference-mediated knockdown of <i>caper</i> in the female germline, results in females laying significantly fewer eggs than their control counterparts. Moreover, this phenotype is exacerbated with age. <i>caper</i> dysfunction also results in partial embryonic and larval lethality. Given that <i>caper</i> is highly conserved across metazoa, these findings may also be relevant to vertebrates.</p>","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":"11 1","pages":""},"PeriodicalIF":2.7,"publicationDate":"2022-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9844462/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9366862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in Understanding the Genetic Mechanisms of Zebrafish Renal Multiciliated Cell Development.","authors":"Hannah M Wesselman, Thanh Khoa Nguyen, Joseph M Chambers, Bridgette E Drummond, Rebecca A Wingert","doi":"10.3390/jdb11010001","DOIUrl":"10.3390/jdb11010001","url":null,"abstract":"<p><p>Cilia are microtubule-based organelles that project from the cell surface. In humans and other vertebrates, possession of a single cilium structure enables an assortment of cellular processes ranging from mechanosensation to fluid propulsion and locomotion. Interestingly, cells can possess a single cilium or many more, where so-called multiciliated cells (MCCs) possess apical membrane complexes with several dozen or even hundreds of motile cilia that beat in a coordinated fashion. Development of MCCs is, therefore, integral to control fluid flow and/or cellular movement in various physiological processes. As such, MCC dysfunction is associated with numerous pathological states. Understanding MCC ontogeny can be used to address congenital birth defects as well as acquired disease conditions. Today, researchers used both in vitro and in vivo experimental models to address our knowledge gaps about MCC specification and differentiation. In this review, we summarize recent discoveries from our lab and others that have illuminated new insights regarding the genetic pathways that direct MCC ontogeny in the embryonic kidney using the power of the zebrafish animal model.</p>","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":"11 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2022-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9844391/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9099767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Primary Cilia Dysfunction in Neurodevelopmental Disorders beyond Ciliopathies.","authors":"Vasiliki Karalis, Kathleen E Donovan, Mustafa Sahin","doi":"10.3390/jdb10040054","DOIUrl":"10.3390/jdb10040054","url":null,"abstract":"<p><p>Primary cilia are specialized, microtubule-based structures projecting from the surface of most mammalian cells. These organelles are thought to primarily act as signaling hubs and sensors, receiving and integrating extracellular cues. Several important signaling pathways are regulated through the primary cilium including Sonic Hedgehog (Shh) and Wnt signaling. Therefore, it is no surprise that mutated genes encoding defective proteins that affect primary cilia function or structure are responsible for a group of disorders collectively termed ciliopathies. The severe neurologic abnormalities observed in several ciliopathies have prompted examination of primary cilia structure and function in other brain disorders. Recently, neuronal primary cilia defects were observed in monogenic neurodevelopmental disorders that were not traditionally considered ciliopathies. The molecular mechanisms of how these genetic mutations cause primary cilia defects and how these defects contribute to the neurologic manifestations of these disorders remain poorly understood. In this review we will discuss monogenic neurodevelopmental disorders that exhibit cilia deficits and summarize findings from studies exploring the role of primary cilia in the brain to shed light into how these deficits could contribute to neurologic abnormalities.</p>","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":"10 4","pages":""},"PeriodicalIF":2.2,"publicationDate":"2022-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9782889/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10428911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Development of the <i>Chimaeroid</i> Pelvic Skeleton and the Evolution of Chondrichthyan Pelvic Fins.","authors":"Jacob B Pears,&nbsp;Carley Tillett,&nbsp;Rui Tahara,&nbsp;Hans C E Larsson,&nbsp;Kate Trinajstic,&nbsp;Catherine A Boisvert","doi":"10.3390/jdb10040053","DOIUrl":"https://doi.org/10.3390/jdb10040053","url":null,"abstract":"<p><p>Pelvic girdles, fins and claspers are evolutionary novelties first recorded in jawed vertebrates. Over the course of the evolution of chondrichthyans (cartilaginous fish) two trends in the morphology of the pelvic skeleton have been suggested to have occurred. These evolutionary shifts involved both an enlargement of the metapterygium (basipterygium) and a transition of fin radial articulation from the pelvic girdle to the metapterygium. To determine how these changes in morphology have occurred it is essential to understand the development of extant taxa as this can indicate potential developmental mechanisms that may have been responsible for these changes. The study of the morphology of the appendicular skeleton across development in chondrichthyans is almost entirely restricted to the historical literature with little contemporary research. Here, we have examined the morphology and development of the pelvic skeleton of a holocephalan chondrichthyan, the elephant shark (<i>Callorhinchus milii</i>), through a combination of dissections, histology, and nanoCT imaging and redescribed the pelvic skeleton of <i>Cladoselache kepleri</i> (NHMUK PV P 9269), a stem holocephalan. To put our findings in their evolutionary context we compare them with the fossil record of chondrichthyans and the literature on pelvic development in elasmobranchs from the late 19th century. Our findings demonstrate that the pelvic skeleton of <i>C. milii</i> initially forms as a single mesenchymal condensation, consisting of the pelvic girdle and a series of fin rays, which fuse to form the basipterygium. The girdle and fin skeleton subsequently segment into distinct components whilst chondrifying. This confirms descriptions of the early pelvic development in <i>Scyliorhinid</i> sharks from the historical literature and suggests that chimaeras and elasmobranchs share common developmental patterns in their pelvic anatomy. Alterations in the location and degree of radial fusion during early development may be the mechanism responsible for changes in pelvic fin morphology over the course of the evolution of both elasmobranchs and holocephalans, which appears to be an example of parallel evolution.</p>","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":"10 4","pages":""},"PeriodicalIF":2.7,"publicationDate":"2022-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9782884/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10428913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}