Journal of Developmental Biology最新文献_第6页

Reproductive-Toxicity-Related Endpoints in C. elegans Are Consistent with Reduced Concern for Dimethylarsinic Acid Exposure Relative to Inorganic Arsenic. 与无机砷相比，虫草中与生殖毒性相关的终点表明二甲基砷酸暴露的关注度降低。

IF 2.2

Journal of Developmental Biology Pub Date : 2023-04-26 DOI: 10.3390/jdb11020018

Jessica A Camacho, Bonnie Welch, Robert L Sprando, Piper R Hunt

{"title":"Reproductive-Toxicity-Related Endpoints in C. elegans Are Consistent with Reduced Concern for Dimethylarsinic Acid Exposure Relative to Inorganic Arsenic.","authors":"Jessica A Camacho, Bonnie Welch, Robert L Sprando, Piper R Hunt","doi":"10.3390/jdb11020018","DOIUrl":"10.3390/jdb11020018","url":null,"abstract":"Exposures to arsenic and mercury are known to pose significant threats to human health; however, the effects specific to organic vs. inorganic forms are not fully understood. Caenorhabditis elegans' (C. elegans) transparent cuticle, along with the conservation of key genetic pathways regulating developmental and reproductive toxicology (DART)-related processes such as germ stem cell renewal and differentiation, meiosis, and embryonic tissue differentiation and growth, support this model's potential to address the need for quicker and more dependable testing methods for DART hazard identification. Organic and inorganic forms of mercury and arsenic had different effects on reproductive-related endpoints in C. elegans, with methylmercury (meHgCl) having effects at lower concentrations than mercury chloride (HgCl2), and sodium arsenite (NaAsO2) having effects at lower concentrations than dimethylarsinic acid (DMA). Progeny to adult ratio changes and germline apoptosis were seen at concentrations that also affected gravid adult gross morphology. For both forms of arsenic tested, germline histone regulation was altered at concentrations below those that affected progeny/adult ratios, while concentrations for these two endpoints were similar for the mercury compounds. These C. elegans findings are consistent with corresponding mammalian data, where available, suggesting that small animal model test systems may help to fill critical data gaps by contributing to weight of evidence assessments.","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":"11 2","pages":""},"PeriodicalIF":2.2,"publicationDate":"2023-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10204422/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9508968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Selecting Normalizers for MicroRNA RT-qPCR Expression Analysis in Murine Preimplantation Embryos and the Associated Conditioned Culture Media. 小鼠着床前胚胎及相关条件培养基中MicroRNA RT-qPCR表达分析的正常化因子选择

IF 2.7

Journal of Developmental Biology Pub Date : 2023-04-04 DOI: 10.3390/jdb11020017

David C Hawke, Andrew J Watson, Dean H Betts

{"title":"Selecting Normalizers for MicroRNA RT-qPCR Expression Analysis in Murine Preimplantation Embryos and the Associated Conditioned Culture Media.","authors":"David C Hawke, Andrew J Watson, Dean H Betts","doi":"10.3390/jdb11020017","DOIUrl":"https://doi.org/10.3390/jdb11020017","url":null,"abstract":"Normalizing RT-qPCR miRNA datasets that encompass numerous preimplantation embryo stages requires the identification of miRNAs that may be used as stable reference genes. A need has also arisen for the normalization of the accompanying conditioned culture media as extracellular miRNAs may serve as biomarkers of embryo developmental competence. Here, we evaluate the stability of six commonly used miRNA normalization candidates, as well as small nuclear U6, using five different means of evaluation (BestKeeper, NormFinder, geNorm, the comparative Delta Ct method and RefFinder comprehensive analysis) to assess their stability throughout murine preimplantation embryo development from the oocyte to the late blastocyst stages, both in whole embryos and the associated conditioned culture media. In descending order of effectiveness, miR-16, miR-191 and miR-106 were identified as the most stable individual reference miRNAs for developing whole CD1 murine preimplantation embryos, while miR-16, miR-106 and miR-103 were ideal for the conditioned culture media. Notably, the widely used U6 reference was among the least appropriate for normalizing both whole embryo and conditioned media miRNA datasets. Incorporating multiple reference miRNAs into the normalization basis via a geometric mean was deemed beneficial, and combinations of each set of stable miRNAs are further recommended, pending validation on a per experiment basis.","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":"11 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2023-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10123758/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9389214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of Pronase versus Manual Dechorionation of Zebrafish Embryos for Small Molecule Treatments. 用于小分子处理的斑马鱼胚胎的Pronase与手动脱氯的比较。

IF 2.7

Journal of Developmental Biology Pub Date : 2023-03-28 DOI: 10.3390/jdb11020016

Eva H Hasegawa, Gist H Farr, Lisa Maves

{"title":"Comparison of Pronase versus Manual Dechorionation of Zebrafish Embryos for Small Molecule Treatments.","authors":"Eva H Hasegawa, Gist H Farr, Lisa Maves","doi":"10.3390/jdb11020016","DOIUrl":"10.3390/jdb11020016","url":null,"abstract":"Zebrafish are a powerful animal model for small molecule screening. Small molecule treatments of zebrafish embryos usually require that the chorion, an acellular envelope enclosing the embryo, is removed in order for chemical compounds to access the embryo from the bath medium. For large-scale studies requiring hundreds of embryos, manual dechorionation, using forceps, can be a time-consuming and limiting process. Pronase is a non-specific protease that is widely used as an enzymatic alternative for dechorionating zebrafish embryos. However, whether pronase treatments alter the effects of subsequent small molecule treatments has not been addressed. Here, we provide a detailed protocol for large-scale pronase dechorionation of zebrafish embryos. We tested whether pronase treatment can influence the efficacy of drug treatments in zebrafish embryos. We used a zebrafish model for Duchenne muscular dystrophy (DMD) to investigate whether the efficacies of trichostatin-A (TSA) or salermide + oxamflatin, small molecule inhibitors known to ameliorate the zebrafish dmd muscle degeneration phenotype, are significantly altered when embryos are treated with pronase versus manual dechorionation. We also tested the effects of pronase on the ability of the anthracycline cancer drug doxorubicin to induce cardiotoxicity in zebrafish embryos. When comparing pronase- versus forceps-dechorionated embryos used in these small molecule treatments, we found no appreciable effects of pronase on animal survival or on the effects of the small molecules. The significant difference that was detected was a small improvement in the ability of salermide + oxamflatin to ameliorate the dmd phenotype in pronase-treated embryos when compared with manual dechorionation. Our study supports the use of pronase treatment as a dechorionation method for zebrafish drug screening experiments.","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":"11 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2023-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10123619/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9382422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Refined Single Cell Landscape of Haematopoiesis in the Mouse Foetal Liver. 小鼠胎肝造血的精细单细胞图谱

IF 2.7

Journal of Developmental Biology Pub Date : 2023-03-23 DOI: 10.3390/jdb11020015

Elena Ceccacci, Emanuela Villa, Fabio Santoro, Saverio Minucci, Christiana Ruhrberg, Alessandro Fantin

{"title":"A Refined Single Cell Landscape of Haematopoiesis in the Mouse Foetal Liver.","authors":"Elena Ceccacci, Emanuela Villa, Fabio Santoro, Saverio Minucci, Christiana Ruhrberg, Alessandro Fantin","doi":"10.3390/jdb11020015","DOIUrl":"10.3390/jdb11020015","url":null,"abstract":"During prenatal life, the foetal liver is colonised by several waves of haematopoietic progenitors to act as the main haematopoietic organ. Single cell (sc) RNA-seq has been used to identify foetal liver cell types via their transcriptomic signature and to compare gene expression patterns as haematopoietic development proceeds. To obtain a refined single cell landscape of haematopoiesis in the foetal liver, we have generated a scRNA-seq dataset from a whole mouse E12.5 liver that includes a larger number of cells than prior datasets at this stage and was obtained without cell type preselection to include all liver cell populations. We combined mining of this dataset with that of previously published datasets at other developmental stages to follow transcriptional dynamics as well as the cell cycle state of developing haematopoietic lineages. Our findings corroborate several prior reports on the timing of liver colonisation by haematopoietic progenitors and the emergence of differentiated lineages and provide further molecular characterisation of each cell population. Extending these findings, we demonstrate the existence of a foetal intermediate haemoglobin profile in the mouse, similar to that previously identified in humans, and a previously unidentified population of primitive erythroid cells in the foetal liver.","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":"11 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2023-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10123705/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9382417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Principles of Zebrafish Nephron Segment Development. 斑马鱼肾细胞段发育的原理。

IF 2.7

Journal of Developmental Biology Pub Date : 2023-03-18 DOI: 10.3390/jdb11010014

Thanh Khoa Nguyen, Madeline Petrikas, Brooke E Chambers, Rebecca A Wingert

引用次数: 4

COMMD10 Is Essential for Neural Plate Development during Embryogenesis. COMMD10 对胚胎发生过程中的神经板发育至关重要

IF 2.7

Journal of Developmental Biology Pub Date : 2023-03-16 DOI: 10.3390/jdb11010013

Khanh P Phan, Panayiotis Pelargos, Alla V Tsytsykova, Erdyni N Tsitsikov, Graham Wiley, Chuang Li, Melissa Bebak, Ian F Dunn

{"title":"COMMD10 Is Essential for Neural Plate Development during Embryogenesis.","authors":"Khanh P Phan, Panayiotis Pelargos, Alla V Tsytsykova, Erdyni N Tsitsikov, Graham Wiley, Chuang Li, Melissa Bebak, Ian F Dunn","doi":"10.3390/jdb11010013","DOIUrl":"10.3390/jdb11010013","url":null,"abstract":"The COMMD (copper metabolism MURR1 domain containing) family includes ten structurally conserved proteins (COMMD1 to COMMD10) in eukaryotic multicellular organisms that are involved in a diverse array of cellular and physiological processes, including endosomal trafficking, copper homeostasis, and cholesterol metabolism, among others. To understand the role of COMMD10 in embryonic development, we used Commd10Tg(Vav1-icre)A2Kio/J mice, where the Vav1-cre transgene is integrated into an intron of the Commd10 gene, creating a functional knockout of Commd10 in homozygous mice. Breeding heterozygous mice produced no COMMD10-deficient (Commd10Null) offspring, suggesting that COMMD10 is required for embryogenesis. Analysis of Commd10Null embryos demonstrated that they displayed stalled development by embryonic day 8.5 (E8.5). Transcriptome analysis revealed that numerous neural crest-specific gene markers had lower expression in mutant versus wild-type (WT) embryos. Specifically, Commd10Null embryos displayed significantly lower expression levels of a number of transcription factors, including a major regulator of the neural crest, Sox10. Moreover, several cytokines/growth factors involved in early embryonic neurogenesis were also lower in mutant embryos. On the other hand, Commd10Null embryos demonstrated higher expression of genes involved in tissue remodeling and regression processes. Taken together, our findings show that Commd10Null embryos die by day E8.5 due to COMMD10-dependent neural crest failure, revealing a new and critical role for COMMD10 in neural development.","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":"11 1","pages":""},"PeriodicalIF":2.7,"publicationDate":"2023-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10051640/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9580880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heme Oxygenase-1 Is Upregulated during Differentiation of Keratinocytes but Its Expression Is Dispensable for Cornification of Murine Epidermis. 血红素加氧酶-1 在角质形成细胞分化过程中上调，但其表达对小鼠表皮的粟粒化是不可或缺的。

IF 2.2

Journal of Developmental Biology Pub Date : 2023-03-10 DOI: 10.3390/jdb11010012

Marta Surbek, Supawadee Sukseree, Attila Placido Sachslehner, Dragan Copic, Bahar Golabi, Ionela Mariana Nagelreiter, Erwin Tschachler, Leopold Eckhart

{"title":"Heme Oxygenase-1 Is Upregulated during Differentiation of Keratinocytes but Its Expression Is Dispensable for Cornification of Murine Epidermis.","authors":"Marta Surbek, Supawadee Sukseree, Attila Placido Sachslehner, Dragan Copic, Bahar Golabi, Ionela Mariana Nagelreiter, Erwin Tschachler, Leopold Eckhart","doi":"10.3390/jdb11010012","DOIUrl":"10.3390/jdb11010012","url":null,"abstract":"The epidermal barrier of mammals is initially formed during embryonic development and continuously regenerated by the differentiation and cornification of keratinocytes in postnatal life. Cornification is associated with the breakdown of organelles and other cell components by mechanisms which are only incompletely understood. Here, we investigated whether heme oxygenase 1 (HO-1), which converts heme into biliverdin, ferrous iron and carbon monoxide, is required for normal cornification of epidermal keratinocytes. We show that HO-1 is transcriptionally upregulated during the terminal differentiation of human keratinocytes in vitro and in vivo. Immunohistochemistry demonstrated expression of HO-1 in the granular layer of the epidermis where keratinocytes undergo cornification. Next, we deleted the Hmox1 gene, which encodes HO-1, by crossing Hmox1-floxed and K14-Cre mice. The epidermis and isolated keratinocytes of the resulting Hmox1f/f K14-Cre mice lacked HO-1 expression. The genetic inactivation of HO-1 did not impair the expression of keratinocyte differentiation markers, loricrin and filaggrin. Likewise, the transglutaminase activity and formation of the stratum corneum were not altered in Hmox1f/f K14-Cre mice, suggesting that HO-1 is dispensable for epidermal cornification. The genetically modified mice generated in this study may be useful for future investigations of the potential roles of epidermal HO-1 in iron metabolism and responses to oxidative stress.","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":"11 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2023-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10058925/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9580878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Scientific Papers by Developmental Biologists in Japan. 日本发育生物学家的科学论文。

IF 2.7

Journal of Developmental Biology Pub Date : 2023-03-10 DOI: 10.3390/jdb11010011

Hideyo Ohuchi, Tsutomu Nohno

引用次数: 0

In Vitro Comparison of Sex-Specific Splicing Efficiencies of fem Pre-mRNA under Monoallelic and Heteroallelic Conditions of csd, a Master Sex-Determining Gene in the Honeybee. 蜜蜂主要性别决定基因csd单等位基因和异等位基因条件下fem Pre-mRNA体外性别特异性剪接效率的比较

IF 2.7

Journal of Developmental Biology Pub Date : 2023-03-10 DOI: 10.3390/jdb11010010

Yukihiro Suzuki, Takafumi Yamada, Masataka G Suzuki

{"title":"In Vitro Comparison of Sex-Specific Splicing Efficiencies of fem Pre-mRNA under Monoallelic and Heteroallelic Conditions of csd, a Master Sex-Determining Gene in the Honeybee.","authors":"Yukihiro Suzuki, Takafumi Yamada, Masataka G Suzuki","doi":"10.3390/jdb11010010","DOIUrl":"https://doi.org/10.3390/jdb11010010","url":null,"abstract":"The sexual fate of honeybees is determined by the complementary sex determination (CSD) model: heterozygosity at a single locus (the CSD locus) determines femaleness, while hemizygosity or homozygosity at the CSD locus determines maleness. The csd gene encodes a splicing factor that regulates sex-specific splicing of the downstream target gene feminizer (fem), which is required for femaleness. The female mode of fem splicing occurs only when csd is present in the heteroallelic condition. To gain insights into how Csd proteins are only activated under the heterozygous allelic composition, we developed an in vitro assay system to evaluate the activity of Csd proteins. Consistent with the CSD model, the co-expression of two csd alleles, both of which lack splicing activity under the single-allele condition, restored the splicing activity that governs the female mode of fem splicing. RNA immunoprecipitation quantitative PCR analyses demonstrated that the CSD protein was specifically enriched in several exonic regions in the fem pre-mRNA, and enrichment in exons 3a and 5 was significantly greater under the heterozygous allelic composition than the single-allelic condition. However, in most cases csd expression under the monoallelic condition was capable of inducing the female mode of fem splicing contrary to the conventional CSD model. In contrast, repression of the male mode of fem splicing was predominant under heteroallelic conditions. These results were reproduced by real-time PCR of endogenous fem expression in female and male pupae. These findings strongly suggest that the heteroallelic composition of csd may be more important for the repression of the male splicing mode than for the induction of the female splicing mode of the fem gene.","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":"11 1","pages":""},"PeriodicalIF":2.7,"publicationDate":"2023-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10057164/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9204494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modeling Podocyte Ontogeny and Podocytopathies with the Zebrafish. 用斑马鱼模拟荚膜细胞的本体发育和荚膜病变

IF 2.2

Journal of Developmental Biology Pub Date : 2023-02-20 DOI: 10.3390/jdb11010009

Bridgette E Drummond, Wesley S Ercanbrack, Rebecca A Wingert

引用次数: 0