Journal of Developmental Biology最新文献_第6页

Defective Neural Stem and Progenitor Cell Proliferation in Neurodevelopmental Disorders. 神经发育障碍的神经干细胞缺陷和祖细胞增殖。

IF 2.5

Journal of Developmental Biology Pub Date : 2025-11-07 DOI: 10.3390/jdb13040040

Aki Shigenaka, Eri Nitta, Tadashi Nakagawa, Makiko Nakagawa, Toru Hosoi

{"title":"Defective Neural Stem and Progenitor Cell Proliferation in Neurodevelopmental Disorders.","authors":"Aki Shigenaka, Eri Nitta, Tadashi Nakagawa, Makiko Nakagawa, Toru Hosoi","doi":"10.3390/jdb13040040","DOIUrl":"10.3390/jdb13040040","url":null,"abstract":"Neurodevelopmental disorders (NDDs), including autism spectrum disorder, intellectual disability, and attention deficit hyperactivity disorder, are increasingly recognized as disorders of early brain construction arising from defects in neural stem and progenitor cell (NSPC) proliferation. NSPCs are responsible for generating the diverse neuronal and glial lineages that establish cortical architecture and neural circuitry; thus, their expansion must be tightly coordinated by intrinsic cell cycle regulators and extrinsic niche-derived cues. Disruption of these mechanisms-through genetic mutations, epigenetic dysregulation, or environmental insults-can perturb the balance between NSPC self-renewal and differentiation, resulting in aberrant brain size and connectivity. Recent advances using animal models and human pluripotent stem cell-derived brain organoids have identified key signaling pathways, including Notch, Wnt, SHH, and PI3K-mTOR, as central hubs integrating proliferative cues, while transcriptional and chromatin regulators such as PAX6, CHD8, SETD5, and ANKRD11 govern gene expression essential for proper NSPC cycling. Furthermore, prenatal exposure to teratogens such as Zika virus infection, valproic acid, or metabolic stress in phenylketonuria can recapitulate proliferation defects and microcephaly, underscoring the vulnerability of NSPCs to environmental perturbation. This review summarizes emerging insights into the molecular and cellular mechanisms by which defective NSPC proliferation contributes to NDD pathogenesis, highlighting convergence among genetic and environmental factors on cell cycle control. A deeper understanding of these pathways may uncover shared therapeutic targets to restore neurodevelopmental trajectories and mitigate disease burden.","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":"13 4","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12641901/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145587584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recapitulating Liver Embryology-Lessons to Be Learned for Liver Diseases. 肝脏胚胎学综述：肝脏疾病的经验教训

IF 2.5

Journal of Developmental Biology Pub Date : 2025-11-04 DOI: 10.3390/jdb13040039

Rui Caetano Oliveira, Sandra Ferreira, Isabel Gonçalves, Maria Fátima Martins

引用次数: 0

Activity-Dependent Increases in Quantal Size at the Drosophila NMJ. 果蝇NMJ数量大小的活动依赖性增加。

IF 2.5

Journal of Developmental Biology Pub Date : 2025-10-28 DOI: 10.3390/jdb13040038

Andrew S Powers, Petar Gajic, Ethan Rittereiser, Kavindra Dasrat, Gregory A Lnenicka

{"title":"Activity-Dependent Increases in Quantal Size at the Drosophila NMJ.","authors":"Andrew S Powers, Petar Gajic, Ethan Rittereiser, Kavindra Dasrat, Gregory A Lnenicka","doi":"10.3390/jdb13040038","DOIUrl":"10.3390/jdb13040038","url":null,"abstract":"We examined whether an increase in synaptic activity resulted in an increase in quantal size at the neuromuscular junction (NMJ) of third-instar Drosophila larvae. Spontaneous miniature excitatory postsynaptic currents (mEPSCs) or miniature excitatory postsynaptic potentials (mEPSPs) were recorded before and after nerve stimulation. We found that prolonged (60 s) or brief (1.25 s) nerve stimulation produced an increase in quantal size; this appears to be a general property of these synapses since it was seen at all four muscle fibers (MFs) used in this study. The effect was examined along Is and Ib terminals by expressing GCaMP in the MF membrane and examining postsynaptic Ca2+ signals produced by spontaneous transmitter release. The activity-dependent increase in quantal size occurred at both Is and Ib terminals, and the increase in frequency and amplitude of quantal events at individual synaptic boutons was correlated. Both the increase in quantal size and frequency were found to be dependent upon an increase in postsynaptic Ca2+, based on studies in which MFs were preinjected with the Ca2+ chelator BAPTA (1,2-Bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid). To examine the effect of postsynaptic activity on glutamate sensitivity, we iontophoresed glutamate pulses at the NMJ and recorded the glutamate-evoked excitatory postsynaptic potentials (gEPSPs). Trains of glutamate pulses produced an increase in gEPSP amplitude; this potentiation was not seen when Ca2+ was eliminated from the bath or after inhibiting calmodulin or CaMKII. The activity-dependent increase in quantal size may result from an increase in postsynaptic sensitivity due to activation of CaMKII.","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":"13 4","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12641814/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145587605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Supra-Physiological Dose of 2-Hydroxyestradiol Impairs Meiotic Progression and Developmental Competence of Mouse Antral Oocytes. 超生理剂量的2-羟基雌二醇损害小鼠心房卵母细胞减数分裂进程和发育能力。

IF 2.5

Journal of Developmental Biology Pub Date : 2025-10-15 DOI: 10.3390/jdb13040037

Valeria Merico, Paola Rebuzzini, Mario Zanoni, Maurizio Zuccotti, Silvia Garagna

{"title":"A Supra-Physiological Dose of 2-Hydroxyestradiol Impairs Meiotic Progression and Developmental Competence of Mouse Antral Oocytes.","authors":"Valeria Merico, Paola Rebuzzini, Mario Zanoni, Maurizio Zuccotti, Silvia Garagna","doi":"10.3390/jdb13040037","DOIUrl":"10.3390/jdb13040037","url":null,"abstract":"Estrogen metabolites (EMs) play a local regulatory role in mammalian ovarian function. Among them, 2-hydroxyestradiol (2-OHE2) exerts dose-dependent effects on reproductive physiology, supporting either normal ovarian processes or contributing to pathological conditions. Specifically, 2-OHE2 modulates ovarian vasculature and progesterone biosynthesis, and at 1-10 nM concentrations, it enhances in vitro developmental competence and blastocyst quality in mouse oocytes. Conversely, doses below 1 nM show no appreciable effects, suggesting the existence of a biological activity threshold. However, the impact of supra-physiological concentrations remains largely unexplored. In this study, we investigated the effects of increasing 2-OHE2 doses (0.05, 0.50, and 5.00 µM) on oocyte meiotic progression and quality. Exposure to 0.50 and 5.00 µM significantly impaired oocyte maturation, while only the highest dose notably reduced the percentage of embryos developing to the blastocyst stage. Morphometric analysis during the GV-to-MII transition revealed altered first polar body morphology, defective asymmetric division, and disruptions in cytoskeletal organization, including enlarged meiotic spindles, increased F-actin cap angles, and aberrant microtubule-organizing centers distribution. These structural alterations were paralleled by distinct changes in cytoplasmic movement velocity patterns observed through time-lapse imaging during meiotic resumption. Together, these findings demonstrate that supra-physiological exposure to 2-OHE2 compromises oocyte maturation and developmental competence by perturbing key cytoskeletal dynamics and cellular architecture necessary for successful meiosis and early embryogenesis.","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":"13 4","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12551066/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145354977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An Integrated Canonical and Non-Canonical Wnt Signaling Network Controls Early Anterior-Posterior Axis Formation in Sea Urchin Embryos. 一个整合的典型和非典型Wnt信号网络控制海胆胚胎早期前后轴的形成。

IF 2.5

Journal of Developmental Biology Pub Date : 2025-10-08 DOI: 10.3390/jdb13040036

Jennifer L Fenner, Boyuan Wang, Cheikhouna Ka, Sujan Gautam, Ryan C Range

{"title":"An Integrated Canonical and Non-Canonical Wnt Signaling Network Controls Early Anterior-Posterior Axis Formation in Sea Urchin Embryos.","authors":"Jennifer L Fenner, Boyuan Wang, Cheikhouna Ka, Sujan Gautam, Ryan C Range","doi":"10.3390/jdb13040036","DOIUrl":"10.3390/jdb13040036","url":null,"abstract":"Wnt signaling is an ancient developmental mechanism that drives the initial specification and patterning of the primary axis in many metazoan embryos. Yet, it is unclear how exactly the various Wnt components interact in most Wnt-mediated developmental processes as well as in the molecular mechanism regulating adult tissue homeostasis. Recent work in invertebrate deuterostome sea urchin embryos indicates that three different Wnt signaling pathways (Wnt/β-catenin, Wnt/JNK, and Wnt/PKC) form an interconnected Wnt signaling network that specifies and patterns the primary anterior-posterior (AP) axis. Here, we detail our current knowledge of this critical regulatory process in sea urchin embryos. We also illustrate examples from a diverse group of metazoans, from cnidarians to vertebrates, that suggest aspects of the sea urchin AP Wnt signaling network are deeply conserved. We explore how the sea urchin is an excellent model to elucidate a detailed molecular understanding of AP axis specification and patterning that can be used for identifying unifying developmental principles across animals.","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":"13 4","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12550998/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145354928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High Concentrations of Non-Esterified Fatty Acids During Bovine In Vitro Fertilisation Are Detrimental for Spermatozoa Quality and Pre-Implantation Embryo Development. 牛体外受精过程中高浓度的非酯化脂肪酸对精子质量和着床前胚胎发育不利。

IF 2.5

Journal of Developmental Biology Pub Date : 2025-10-05 DOI: 10.3390/jdb13040035

Abdullah F Idriss, Edward J Okello, Roger G Sturmey, Miguel A Velazquez

{"title":"High Concentrations of Non-Esterified Fatty Acids During Bovine In Vitro Fertilisation Are Detrimental for Spermatozoa Quality and Pre-Implantation Embryo Development.","authors":"Abdullah F Idriss, Edward J Okello, Roger G Sturmey, Miguel A Velazquez","doi":"10.3390/jdb13040035","DOIUrl":"10.3390/jdb13040035","url":null,"abstract":"High non-esterified fatty acids (NEFAs) during negative energy balance in dairy cattle can impair reproduction. While their effects on oocyte maturation and preimplantation embryo development are known, their impact during fertilisation is largely unexplored. This study examined the effects of high NEFA exposure exclusively during in vitro fertilisation (IVF). Bovine oocytes were matured in vitro and fertilised under physiological or high NEFA concentrations. High NEFA concentrations decreased fertilisation, cleavage, and blastocyst rates. Reactive oxygen species production in zygotes was not affected, but blastocysts derived from the High-NEFA group had fewer cells. Spermatozoa exposed to high NEFA concentrations exhibited increased plasma membrane and acrosome damage, higher DNA fragmentation, and reduced mitochondrial membrane potential. The expression of H3K27me3, a repressive histone mark normally erased from fertilisation to embryonic genome activation, was higher in 2-cell than in 4-cell embryos on day 2 after IVF, but only in the High-NEFA group. This delayed H3K27me3 loss, along with increased DNA damage, could partially explain the reduced blastocyst formation observed. In conclusion, high NEFA concentrations can impair pre-implantation embryo development during zygote formation, potentially via effects on both the oocyte and spermatozoon. The latter warrants further investigation using an intracytoplasmic sperm injection model.","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":"13 4","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12550938/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145354931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the Regulation of Tmem182 Gene Expression in the Context of Retinoid X Receptor Signaling. 维甲酸X受体信号传导对Tmem182基因表达调控的研究

IF 2.5

Journal of Developmental Biology Pub Date : 2025-09-24 DOI: 10.3390/jdb13040034

Saadia Khilji, Munerah Hamed, Jihong Chen, Qiao Li

{"title":"Exploring the Regulation of Tmem182 Gene Expression in the Context of Retinoid X Receptor Signaling.","authors":"Saadia Khilji, Munerah Hamed, Jihong Chen, Qiao Li","doi":"10.3390/jdb13040034","DOIUrl":"10.3390/jdb13040034","url":null,"abstract":"We have previously established that bexarotene, a clinically approved agonist of retinoid X receptor (RXR), promotes the differentiation and fusion of skeletal myoblasts. We have also analyzed the genomic programs underlying rexinoid-enhanced myogenic differentiation to identify novel regulatory pathways. As such, we observed a significant upregulation of a transcript encoding a predicted transmembrane protein, Tmem182, during C2C12 myoblast differentiation. Despite the documentation of Tmem182 expression in skeletal muscles, its regulation had yet to be explored. Here, we show that Tmem182 gene expression is markedly augmented in early myoblast differentiation and further enhanced by RXR signaling. In addition, Tmem182 expression is specific to muscle tissues and related to muscle master regulator MyoD. We found that MyoD and histone acetyltransferase p300 are bound to the Tmem182 promoter, and Tmem182 expression is p300-dependent. Thus, our data display a putative epigenetic signature associated with p300 and histone acetylation in rexinoid-responsive locus activation and transcription of myogenic targets.","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":"13 4","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12550946/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145354934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Signaling Pathways in Human Blastocyst Development: From Molecular Mechanisms to In Vitro Optimization. 人囊胚发育中的信号通路：从分子机制到体外优化。

IF 2.5

Journal of Developmental Biology Pub Date : 2025-09-09 DOI: 10.3390/jdb13030033

Yan Jiao, Jiapeng Liu, Congge Li, Yuexin Hu, Sanjun Zhao

引用次数: 0

Zebrafish Unga Is Required for Genomic Maintenance upon Genotoxic Stress and Male Fertility. 斑马鱼Unga基因在基因毒性应激和雄性生育能力下的基因组维持是必需的。

IF 2.4

Journal of Developmental Biology Pub Date : 2025-09-02 DOI: 10.3390/jdb13030032

Latifa Kazzazy, Flóra Huba, Bálint Lóránt Hausz, Dávid Mező, Viktória Perey-Simon, Bálint Jezsó, Abdulrahman Seddik, Zoran Marinović, Judit Tóth, Angéla Békési, Beáta G Vértessy, Máté Varga

引用次数: 0

Profilin and Non-Canonical Wnt Signaling: Coordinating Cytoskeletal Dynamics from Development to Disease. Profilin和Non-Canonical Wnt信号：从发育到疾病的协调细胞骨架动力学。

IF 2.5

Journal of Developmental Biology Pub Date : 2025-09-01 DOI: 10.3390/jdb13030031

Samira Alam, Danielle Duncan, Sharmin Hasan

{"title":"Profilin and Non-Canonical Wnt Signaling: Coordinating Cytoskeletal Dynamics from Development to Disease.","authors":"Samira Alam, Danielle Duncan, Sharmin Hasan","doi":"10.3390/jdb13030031","DOIUrl":"10.3390/jdb13030031","url":null,"abstract":"Vertebrate embryonic development relies on tightly regulated signaling pathways that guide morphogenesis, cell fate specification, and tissue organization. Among these, the Wnt signaling pathway plays a central role, orchestrating key developmental events. The non-canonical Wnt pathways, including the Planar Cell Polarity and Wnt/Ca2+ branches, are especially critical for regulating cytoskeletal dynamics during gastrulation. Recent studies highlight that these pathways interface with cytoskeletal effectors to control actin remodeling in response to extracellular cues. One such effector is Profilin, a small, evolutionarily conserved actin-binding protein that modulates actin polymerization and cellular architecture. Profilins, particularly Profilin1 and 2, are known to interact with Daam1, a formin protein downstream of PCP signaling, thereby linking Wnt signals to actin cytoskeletal regulation. Emerging evidence suggests that Profilins are active signaling intermediates that contribute to morphogenetic processes. Their context-dependent interactions and differential expression across species also suggest that they play specialized roles in development and disease. This review synthesizes the current understanding of Profilin's role in non-canonical Wnt signaling, examining its molecular interactions and contributions to cytoskeletal control during development. By integrating data across model systems, we aim to clarify how Profilins function at the intersection of signaling and cytoskeletal dynamics, with implications for both developmental biology and disease pathogenesis.","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":"13 3","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12452346/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145113332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0