Journal of Developmental Biology最新文献_第6页

A Refined Single Cell Landscape of Haematopoiesis in the Mouse Foetal Liver. 小鼠胎肝造血的精细单细胞图谱

IF 2.7

Journal of Developmental Biology Pub Date : 2023-03-23 DOI: 10.3390/jdb11020015

Elena Ceccacci, Emanuela Villa, Fabio Santoro, Saverio Minucci, Christiana Ruhrberg, Alessandro Fantin

{"title":"A Refined Single Cell Landscape of Haematopoiesis in the Mouse Foetal Liver.","authors":"Elena Ceccacci, Emanuela Villa, Fabio Santoro, Saverio Minucci, Christiana Ruhrberg, Alessandro Fantin","doi":"10.3390/jdb11020015","DOIUrl":"10.3390/jdb11020015","url":null,"abstract":"During prenatal life, the foetal liver is colonised by several waves of haematopoietic progenitors to act as the main haematopoietic organ. Single cell (sc) RNA-seq has been used to identify foetal liver cell types via their transcriptomic signature and to compare gene expression patterns as haematopoietic development proceeds. To obtain a refined single cell landscape of haematopoiesis in the foetal liver, we have generated a scRNA-seq dataset from a whole mouse E12.5 liver that includes a larger number of cells than prior datasets at this stage and was obtained without cell type preselection to include all liver cell populations. We combined mining of this dataset with that of previously published datasets at other developmental stages to follow transcriptional dynamics as well as the cell cycle state of developing haematopoietic lineages. Our findings corroborate several prior reports on the timing of liver colonisation by haematopoietic progenitors and the emergence of differentiated lineages and provide further molecular characterisation of each cell population. Extending these findings, we demonstrate the existence of a foetal intermediate haemoglobin profile in the mouse, similar to that previously identified in humans, and a previously unidentified population of primitive erythroid cells in the foetal liver.","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":"11 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2023-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10123705/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9382417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Principles of Zebrafish Nephron Segment Development. 斑马鱼肾细胞段发育的原理。

IF 2.7

Journal of Developmental Biology Pub Date : 2023-03-18 DOI: 10.3390/jdb11010014

Thanh Khoa Nguyen, Madeline Petrikas, Brooke E Chambers, Rebecca A Wingert

引用次数: 4

COMMD10 Is Essential for Neural Plate Development during Embryogenesis. COMMD10 对胚胎发生过程中的神经板发育至关重要

IF 2.7

Journal of Developmental Biology Pub Date : 2023-03-16 DOI: 10.3390/jdb11010013

Khanh P Phan, Panayiotis Pelargos, Alla V Tsytsykova, Erdyni N Tsitsikov, Graham Wiley, Chuang Li, Melissa Bebak, Ian F Dunn

{"title":"COMMD10 Is Essential for Neural Plate Development during Embryogenesis.","authors":"Khanh P Phan, Panayiotis Pelargos, Alla V Tsytsykova, Erdyni N Tsitsikov, Graham Wiley, Chuang Li, Melissa Bebak, Ian F Dunn","doi":"10.3390/jdb11010013","DOIUrl":"10.3390/jdb11010013","url":null,"abstract":"The COMMD (copper metabolism MURR1 domain containing) family includes ten structurally conserved proteins (COMMD1 to COMMD10) in eukaryotic multicellular organisms that are involved in a diverse array of cellular and physiological processes, including endosomal trafficking, copper homeostasis, and cholesterol metabolism, among others. To understand the role of COMMD10 in embryonic development, we used Commd10Tg(Vav1-icre)A2Kio/J mice, where the Vav1-cre transgene is integrated into an intron of the Commd10 gene, creating a functional knockout of Commd10 in homozygous mice. Breeding heterozygous mice produced no COMMD10-deficient (Commd10Null) offspring, suggesting that COMMD10 is required for embryogenesis. Analysis of Commd10Null embryos demonstrated that they displayed stalled development by embryonic day 8.5 (E8.5). Transcriptome analysis revealed that numerous neural crest-specific gene markers had lower expression in mutant versus wild-type (WT) embryos. Specifically, Commd10Null embryos displayed significantly lower expression levels of a number of transcription factors, including a major regulator of the neural crest, Sox10. Moreover, several cytokines/growth factors involved in early embryonic neurogenesis were also lower in mutant embryos. On the other hand, Commd10Null embryos demonstrated higher expression of genes involved in tissue remodeling and regression processes. Taken together, our findings show that Commd10Null embryos die by day E8.5 due to COMMD10-dependent neural crest failure, revealing a new and critical role for COMMD10 in neural development.","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":"11 1","pages":""},"PeriodicalIF":2.7,"publicationDate":"2023-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10051640/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9580880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heme Oxygenase-1 Is Upregulated during Differentiation of Keratinocytes but Its Expression Is Dispensable for Cornification of Murine Epidermis. 血红素加氧酶-1 在角质形成细胞分化过程中上调，但其表达对小鼠表皮的粟粒化是不可或缺的。

IF 2.2

Journal of Developmental Biology Pub Date : 2023-03-10 DOI: 10.3390/jdb11010012

Marta Surbek, Supawadee Sukseree, Attila Placido Sachslehner, Dragan Copic, Bahar Golabi, Ionela Mariana Nagelreiter, Erwin Tschachler, Leopold Eckhart

{"title":"Heme Oxygenase-1 Is Upregulated during Differentiation of Keratinocytes but Its Expression Is Dispensable for Cornification of Murine Epidermis.","authors":"Marta Surbek, Supawadee Sukseree, Attila Placido Sachslehner, Dragan Copic, Bahar Golabi, Ionela Mariana Nagelreiter, Erwin Tschachler, Leopold Eckhart","doi":"10.3390/jdb11010012","DOIUrl":"10.3390/jdb11010012","url":null,"abstract":"The epidermal barrier of mammals is initially formed during embryonic development and continuously regenerated by the differentiation and cornification of keratinocytes in postnatal life. Cornification is associated with the breakdown of organelles and other cell components by mechanisms which are only incompletely understood. Here, we investigated whether heme oxygenase 1 (HO-1), which converts heme into biliverdin, ferrous iron and carbon monoxide, is required for normal cornification of epidermal keratinocytes. We show that HO-1 is transcriptionally upregulated during the terminal differentiation of human keratinocytes in vitro and in vivo. Immunohistochemistry demonstrated expression of HO-1 in the granular layer of the epidermis where keratinocytes undergo cornification. Next, we deleted the Hmox1 gene, which encodes HO-1, by crossing Hmox1-floxed and K14-Cre mice. The epidermis and isolated keratinocytes of the resulting Hmox1f/f K14-Cre mice lacked HO-1 expression. The genetic inactivation of HO-1 did not impair the expression of keratinocyte differentiation markers, loricrin and filaggrin. Likewise, the transglutaminase activity and formation of the stratum corneum were not altered in Hmox1f/f K14-Cre mice, suggesting that HO-1 is dispensable for epidermal cornification. The genetically modified mice generated in this study may be useful for future investigations of the potential roles of epidermal HO-1 in iron metabolism and responses to oxidative stress.","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":"11 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2023-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10058925/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9580878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Scientific Papers by Developmental Biologists in Japan. 日本发育生物学家的科学论文。

IF 2.7

Journal of Developmental Biology Pub Date : 2023-03-10 DOI: 10.3390/jdb11010011

Hideyo Ohuchi, Tsutomu Nohno

引用次数: 0

In Vitro Comparison of Sex-Specific Splicing Efficiencies of fem Pre-mRNA under Monoallelic and Heteroallelic Conditions of csd, a Master Sex-Determining Gene in the Honeybee. 蜜蜂主要性别决定基因csd单等位基因和异等位基因条件下fem Pre-mRNA体外性别特异性剪接效率的比较

IF 2.7

Journal of Developmental Biology Pub Date : 2023-03-10 DOI: 10.3390/jdb11010010

Yukihiro Suzuki, Takafumi Yamada, Masataka G Suzuki

{"title":"In Vitro Comparison of Sex-Specific Splicing Efficiencies of fem Pre-mRNA under Monoallelic and Heteroallelic Conditions of csd, a Master Sex-Determining Gene in the Honeybee.","authors":"Yukihiro Suzuki, Takafumi Yamada, Masataka G Suzuki","doi":"10.3390/jdb11010010","DOIUrl":"https://doi.org/10.3390/jdb11010010","url":null,"abstract":"The sexual fate of honeybees is determined by the complementary sex determination (CSD) model: heterozygosity at a single locus (the CSD locus) determines femaleness, while hemizygosity or homozygosity at the CSD locus determines maleness. The csd gene encodes a splicing factor that regulates sex-specific splicing of the downstream target gene feminizer (fem), which is required for femaleness. The female mode of fem splicing occurs only when csd is present in the heteroallelic condition. To gain insights into how Csd proteins are only activated under the heterozygous allelic composition, we developed an in vitro assay system to evaluate the activity of Csd proteins. Consistent with the CSD model, the co-expression of two csd alleles, both of which lack splicing activity under the single-allele condition, restored the splicing activity that governs the female mode of fem splicing. RNA immunoprecipitation quantitative PCR analyses demonstrated that the CSD protein was specifically enriched in several exonic regions in the fem pre-mRNA, and enrichment in exons 3a and 5 was significantly greater under the heterozygous allelic composition than the single-allelic condition. However, in most cases csd expression under the monoallelic condition was capable of inducing the female mode of fem splicing contrary to the conventional CSD model. In contrast, repression of the male mode of fem splicing was predominant under heteroallelic conditions. These results were reproduced by real-time PCR of endogenous fem expression in female and male pupae. These findings strongly suggest that the heteroallelic composition of csd may be more important for the repression of the male splicing mode than for the induction of the female splicing mode of the fem gene.","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":"11 1","pages":""},"PeriodicalIF":2.7,"publicationDate":"2023-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10057164/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9204494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modeling Podocyte Ontogeny and Podocytopathies with the Zebrafish. 用斑马鱼模拟荚膜细胞的本体发育和荚膜病变

IF 2.2

Journal of Developmental Biology Pub Date : 2023-02-20 DOI: 10.3390/jdb11010009

Bridgette E Drummond, Wesley S Ercanbrack, Rebecca A Wingert

引用次数: 0

Neurogenin 2 and Neuronal Differentiation 1 Control Proper Development of the Chick Trigeminal Ganglion and Its Nerve Branches. 神经原素2和神经元分化1控制鸡三叉神经节及其神经分支的正常发育。

IF 2.7

Journal of Developmental Biology Pub Date : 2023-02-19 DOI: 10.3390/jdb11010008

Parinaz Bina, Margaret A Hines, Johena Sanyal, Lisa A Taneyhill

{"title":"Neurogenin 2 and Neuronal Differentiation 1 Control Proper Development of the Chick Trigeminal Ganglion and Its Nerve Branches.","authors":"Parinaz Bina, Margaret A Hines, Johena Sanyal, Lisa A Taneyhill","doi":"10.3390/jdb11010008","DOIUrl":"https://doi.org/10.3390/jdb11010008","url":null,"abstract":"The trigeminal ganglion contains the cell bodies of sensory neurons comprising cranial nerve V, which relays information related to pain, touch, and temperature from the face and head to the brain. Like other cranial ganglia, the trigeminal ganglion is composed of neuronal derivatives of two critical embryonic cell types, neural crest and placode cells. Neurogenesis within the cranial ganglia is promoted by Neurogenin 2 (Neurog2), which is expressed in trigeminal placode cells and their neuronal derivatives, and transcriptionally activates neuronal differentiation genes such as Neuronal Differentiation 1 (NeuroD1). Little is known, however, about the role of Neurog2 and NeuroD1 during chick trigeminal gangliogenesis. To address this, we depleted Neurog2 and NeuroD1 from trigeminal placode cells with morpholinos and demonstrated that Neurog2 and NeuroD1 influence trigeminal ganglion development. While knockdown of both Neurog2 and NeuroD1 affected innervation of the eye, Neurog2 and NeuroD1 had opposite effects on ophthalmic nerve branch organization. Taken together, our results highlight, for the first time, functional roles for Neurog2 and NeuroD1 during chick trigeminal gangliogenesis. These studies shed new light on the molecular mechanisms underlying trigeminal ganglion formation and may also provide insight into general cranial gangliogenesis and diseases of the peripheral nervous system.","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":"11 1","pages":""},"PeriodicalIF":2.7,"publicationDate":"2023-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9953625/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9233567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Introduction to the Development of Skin in Vertebrates. 脊椎动物皮肤发育概论。

IF 2.7

Journal of Developmental Biology Pub Date : 2023-01-31 DOI: 10.3390/jdb11010007

Lorenzo Alibardi

引用次数: 0

The Complex Bridge between Aquatic and Terrestrial Life: Skin Changes during Development of Amphibians. 水生生物与陆生生物之间的复杂桥梁：两栖动物发育过程中的皮肤变化

IF 2.2

Journal of Developmental Biology Pub Date : 2023-01-30 DOI: 10.3390/jdb11010006

Esra Akat Çömden, Melodi Yenmiş, Berna Çakır

引用次数: 0