Journal of Developmental Biology最新文献_第5页

Andy Golden: Mentorship through the Years 安迪·戈尔登:多年的导师

Journal of Developmental Biology Pub Date : 2023-11-03 DOI: 10.3390/jdb11040041

Anna K. Allen, Xiaofei Bai, Edward S. Davis, Amy Fabritius, Aimee Jaramillo-Lambert, Peter A. Kropp, Christopher T. Richie, Jill M. Schumacher, Sanjay Shrestha, Kathryn Stein, Ann K. Corsi

引用次数: 0

Use of Farnesyl Transferase Inhibitors in an Ageing Model in Drosophila 法尼基转移酶抑制剂在果蝇衰老模型中的应用

Journal of Developmental Biology Pub Date : 2023-10-29 DOI: 10.3390/jdb11040040

Annely Brandt, Roman Petrovsky, Maria Kriebel, Jörg Großhans

{"title":"Use of Farnesyl Transferase Inhibitors in an Ageing Model in Drosophila","authors":"Annely Brandt, Roman Petrovsky, Maria Kriebel, Jörg Großhans","doi":"10.3390/jdb11040040","DOIUrl":"https://doi.org/10.3390/jdb11040040","url":null,"abstract":"The presence of farnesylated proteins at the inner nuclear membrane (INM), such as the Lamins or Kugelkern in Drosophila, leads to specific changes in the nuclear morphology and accelerated ageing on the organismal level reminiscent of the Hutchinson–Gilford progeria syndrome (HGPS). Farnesyl transferase inhibitors (FTIs) can suppress the phenotypes of the nuclear morphology in cultured fibroblasts from HGPS patients and cultured cells overexpressing farnesylated INM proteins. Similarly, FTIs have been reported to suppress the shortened lifespan in model organisms. Here, we report an experimental system combining cell culture and Drosophila flies for testing the activity of substances on the HGPS-like nuclear morphology and lifespan, with FTIs as an experimental example. Consistent with previous reports, we show that FTIs were able to ameliorate the nuclear phenotypes induced by the farnesylated nuclear proteins Progerin, Kugelkern, or truncated Lamin B in cultured cells. The subsequent validation in Drosophila lifespan assays demonstrated the applicability of the experimental system: treating adult Drosophila with the FTI ABT-100 reversed the nuclear phenotypes and extended the lifespan of experimentally induced short-lived flies. Since kugelkern-expressing flies have a significantly shorter average lifespan, half the time is needed for testing substances in the lifespan assay.","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":"47 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136135846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The New Nematicide Cyclobutrifluram Targets the Mitochondrial Succinate Dehydrogenase Complex in Caenorhabditis elegans. 新型线虫环丁氟仑靶向秀丽隐杆线虫线粒体琥珀酸脱氢酶复合体。

IF 2.7

Journal of Developmental Biology Pub Date : 2023-10-19 DOI: 10.3390/jdb11040039

Fariba Heydari, David Rodriguez-Crespo, Chantal Wicky

{"title":"The New Nematicide Cyclobutrifluram Targets the Mitochondrial Succinate Dehydrogenase Complex in Caenorhabditis elegans.","authors":"Fariba Heydari, David Rodriguez-Crespo, Chantal Wicky","doi":"10.3390/jdb11040039","DOIUrl":"10.3390/jdb11040039","url":null,"abstract":"Today, agriculture around the world is challenged by parasitic nematode infections. Plant-parasitic nematodes (PPNs) can cause significant damage and crop loss and are a threat to food security. For a long time, the management of PPN infection has relied on nematicides that impact not only parasitic nematodes but also other organisms. More recently, new nematicides have been developed that appear to specifically target PPN. Cyclobutrifluram belongs to this new category of nematicides. Using the nematode Caenorhabditis elegans as a model organism, we show here that cyclobutrifluram strongly impacts the survival and fertility rates of the worm by decreasing the number of germ cells. Furthermore, using a genetic approach, we demonstrate that cyclobutrifluram functions by inhibiting the mitochondrial succinate dehydrogenase (SDH) complex. Transcriptomic analysis revealed a strong response to cyclobutrifluram exposure. Among the deregulated genes, we found genes coding for detoxifying proteins, such as cytochrome P450s and UDP-glucuronosyl transferases (UGTs). Overall, our study contributes to the understanding of the molecular mode of action of cyclobutrifluram, to the finding of new approaches against nematicide resistance, and to the discovery of novel nematicides. Furthermore, this study confirms that C. elegans is a suitable model organism to study the mode of action of nematicides.","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":"11 4","pages":""},"PeriodicalIF":2.7,"publicationDate":"2023-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10594496/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49690788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regulation and Function of FOXC1 in Osteoblasts. FOXC1在成骨细胞中的调节和功能。

IF 2.7

Journal of Developmental Biology Pub Date : 2023-09-19 DOI: 10.3390/jdb11030038

Sarocha Suthon, Jianjian Lin, Rachel S Perkins, Gustavo A Miranda-Carboni, Susan A Krum

{"title":"Regulation and Function of FOXC1 in Osteoblasts.","authors":"Sarocha Suthon, Jianjian Lin, Rachel S Perkins, Gustavo A Miranda-Carboni, Susan A Krum","doi":"10.3390/jdb11030038","DOIUrl":"https://doi.org/10.3390/jdb11030038","url":null,"abstract":"Estrogens, which bind to estrogen receptor alpha (ERα), are important for proper bone mineral density. When women go through menopause, estrogen levels decrease, and there is a decrease in bone quality, along with an increased risk for fractures. We previously identified an enhancer near FOXC1 as the most significantly enriched binding site for estrogen receptor alpha (ERα) in osteoblasts. FOXC1 is a transcription factor belonging to a large group of proteins known as forkhead box genes and is an important regulator of bone formation. Here, we demonstrate that 17β-estradiol (E2) increases the mRNA and protein levels of FOXC1 in primary mouse and human osteoblasts. GATA4 is a pioneer factor for ERα and it is also recruited to enhancers near Foxc1. Knockdown of Gata4 in mouse osteoblasts in vitro decreases Foxc1 expression as does knockout of Gata4 in vivo. Functionally, GATA4 and FOXC1 interact and regulate osteoblast proteins such as RUNX2, as demonstrated by ChIP-reChIP and luciferase assays. The most enriched motif in GATA4 binding sites from ChIP-seq is for FOXC1, supporting the notion that GATA4 and FOXC1 cooperate in regulating osteoblast differentiation. Together, these data demonstrate the interactions of the transcription factors ERα, GATA4, and FOXC1 to regulate each other's expression and other osteoblast differentiation genes.","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":"11 3","pages":""},"PeriodicalIF":2.7,"publicationDate":"2023-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10531946/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41115526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identifying Molecular Roadblocks for Transcription Factor-Induced Cellular Reprogramming In Vivo by Using C. elegans as a Model Organism. 以秀丽隐杆线虫为模型生物鉴定转录因子诱导的体内细胞重编程的分子障碍。

IF 2.7

Journal of Developmental Biology Pub Date : 2023-08-31 DOI: 10.3390/jdb11030037

Ismail Özcan, Baris Tursun

{"title":"Identifying Molecular Roadblocks for Transcription Factor-Induced Cellular Reprogramming In Vivo by Using C. elegans as a Model Organism.","authors":"Ismail Özcan, Baris Tursun","doi":"10.3390/jdb11030037","DOIUrl":"https://doi.org/10.3390/jdb11030037","url":null,"abstract":"Generating specialized cell types via cellular transcription factor (TF)-mediated reprogramming has gained high interest in regenerative medicine due to its therapeutic potential to repair tissues and organs damaged by diseases or trauma. Organ dysfunction or improper tissue functioning might be restored by producing functional cells via direct reprogramming, also known as transdifferentiation. Regeneration by converting the identity of available cells in vivo to the desired cell fate could be a strategy for future cell replacement therapies. However, the generation of specific cell types via reprogramming is often restricted due to cell fate-safeguarding mechanisms that limit or even block the reprogramming of the starting cell type. Nevertheless, efficient reprogramming to generate homogeneous cell populations with the required cell type's proper molecular and functional identity is critical. Incomplete reprogramming will lack therapeutic potential and can be detrimental as partially reprogrammed cells may acquire undesired properties and develop into tumors. Identifying and evaluating molecular barriers will improve reprogramming efficiency to reliably establish the target cell identity. In this review, we summarize how using the nematode C. elegans as an in vivo model organism identified molecular barriers of TF-mediated reprogramming. Notably, many identified molecular factors have a high degree of conservation and were subsequently shown to block TF-induced reprogramming of mammalian cells.","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":"11 3","pages":""},"PeriodicalIF":2.7,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10531806/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41115525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Current Advances in Bovine In Vitro Maturation and Embryo Production Using Different Antioxidants: A Review. 使用不同抗氧化剂的牛体外成熟和胚胎生产的最新进展：综述。

IF 2.7

Journal of Developmental Biology Pub Date : 2023-08-29 DOI: 10.3390/jdb11030036

Roksana Naspinska, Maria Helena Moreira da Silva, Fernando Moreira da Silva

引用次数: 0

Immunolocalization of Some Epidermal Proteins and Glycoproteins in the Growing Skin of the Australian Lungfish (Neoceratodus forsteri). 澳大利亚肺鱼(Neoceratodus forsteri)生长皮肤中一些表皮蛋白和糖蛋白的免疫定位。

IF 2.7

Journal of Developmental Biology Pub Date : 2023-08-14 DOI: 10.3390/jdb11030035

Lorenzo Alibardi

{"title":"Immunolocalization of Some Epidermal Proteins and Glycoproteins in the Growing Skin of the Australian Lungfish (Neoceratodus forsteri).","authors":"Lorenzo Alibardi","doi":"10.3390/jdb11030035","DOIUrl":"https://doi.org/10.3390/jdb11030035","url":null,"abstract":"Here we report the immunolocalization of mucin, nestin, elastin and three glycoproteins involved in tissue mineralization in small and large juveniles of Neoceratodus forsteri. Both small and larger juvenile epidermis are mucogenic and contain a diffuse immunolabeling for nestin. Sparse PCNA-labeled cells, indicating proliferation, are found in basal and suprabasal epidermal layers. No scales are formed in small juveniles but are present in a 5 cm long juvenile and in larger juveniles. Elastin and a mineralizing matrix are localized underneath the basement membrane of the tail epidermis where lepidotriches are forming. The latter appears as \"circular bodies\" in cross sections and are made of elongated cells surrounding a central amorphous area containing collagen and elastin-like proteins that undergo calcification as evidenced using the von Kossa staining. However, the first calcification sites are the coniform teeth of the small juveniles of 2-3 cm in length. In the superficial dermis of juveniles (16-26 cm in length) where scales are formed, the spinulated outer bony layer (squamulin) of the elasmoid scales contains osteonectin, alkaline phosphatase, osteopontin, and calcium deposits that are instead absent in the underlying layer of elasmodin. In particular, these glycoproteins are localized along the scale margin in juveniles where scales grow, as indicated by the presence of PCNA-labeled cells (proliferating). These observations suggest a continuous deposition of new bone during the growth of the scales, possibly under the action of these mineralizing glycoproteins, like in the endoskeleton of terrestrial vertebrates.","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":"11 3","pages":""},"PeriodicalIF":2.7,"publicationDate":"2023-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10443291/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10060891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Vasa, Piwi, and Pl10 Expression during Sexual Maturation and Asexual Reproduction in the Annelid Pristina longiseta. Vasa, Piwi和Pl10在环节动物Pristina longiseta性成熟和无性繁殖中的表达。

IF 2.2

Journal of Developmental Biology Pub Date : 2023-08-09 DOI: 10.3390/jdb11030034

Roman P Kostyuchenko, Natalia P Smirnova

{"title":"Vasa, Piwi, and Pl10 Expression during Sexual Maturation and Asexual Reproduction in the Annelid Pristina longiseta.","authors":"Roman P Kostyuchenko, Natalia P Smirnova","doi":"10.3390/jdb11030034","DOIUrl":"10.3390/jdb11030034","url":null,"abstract":"Naidids are tiny, transparent freshwater oligochaetes, which are well known for their ability to propagate asexually. Despite the fact that sexually mature individuals and cocoons with embryos are sometimes found in nature, in long-period laboratory cultures, worms reproduce agametically only. In this paper, we showed, for the first time, the expression of Vasa, Piwi, and Pl10 homologs in mature Pristina longiseta worms with well-developed reproductive system structures and germ cells. Although the animals have been propagated asexually by paratomic fission for over 20 years in our lab, some individuals become sexualized under standard conditions for our laboratory culture and demonstrate various stages of maturation. The fully matured animals developed a complete set of sexual apparatus including spermatheca, atrium, seminal vesicles, and ovisac. They also had a clitellum and were able to form cocoons. The cues for the initiation of sexual maturation are still unknown for P. longiseta; nevertheless, our data suggest that the laboratory strain of P. longiseta maintains the ability to become fully sexually mature and to establish germline products even after a long period of agametic reproduction. On the other hand, many of the sexualized worms formed a fission zone and continued to reproduce asexually. Thus, in this species, the processes of asexual reproduction and sexual maturation do not preclude each other, and Vasa, Piwi, and Pl10 homologs are expressed in both somatic and germline tissue including the posterior growth zone, fission zone, nervous system, germline cells, and gametes.","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":"11 3","pages":""},"PeriodicalIF":2.2,"publicationDate":"2023-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10443295/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10058805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Decreased Expression of Pulmonary Homeobox NKX2.1 and Surfactant Protein C in Developing Lungs That Over-Express Receptors for Advanced Glycation End-Products (RAGE). 过度表达高级糖化终产物受体（RAGE）的发育中肺部同源染色体 NKX2.1 和表面活性蛋白 C 表达减少

IF 2.2

Journal of Developmental Biology Pub Date : 2023-07-15 DOI: 10.3390/jdb11030033

Derek M Clarke, Katrina L Curtis, Ryan A Wendt, Brendan M Stapley, Evan T Clark, Nathan Beckett, Kennedy M Campbell, Juan A Arroyo, Paul R Reynolds

{"title":"Decreased Expression of Pulmonary Homeobox NKX2.1 and Surfactant Protein C in Developing Lungs That Over-Express Receptors for Advanced Glycation End-Products (RAGE).","authors":"Derek M Clarke, Katrina L Curtis, Ryan A Wendt, Brendan M Stapley, Evan T Clark, Nathan Beckett, Kennedy M Campbell, Juan A Arroyo, Paul R Reynolds","doi":"10.3390/jdb11030033","DOIUrl":"10.3390/jdb11030033","url":null,"abstract":"Receptors for advanced glycation end-products (RAGE) are multi-ligand cell surface receptors of the immunoglobin superfamily prominently expressed by lung epithelium. Previous experiments demonstrated that over-expression of RAGE by murine alveolar epithelium throughout embryonic development causes neonatal lethality coincident with significant lung hypoplasia. In the current study, we evaluated the expression of NKX2.1 (also referred to as TTF-1), a homeodomain-containing transcription factor critical for branching morphogenesis, in mice that differentially expressed RAGE. We also contextualized NKX2.1 expression with the abundance of FoxA2, a winged double helix DNA binding protein that influences respiratory epithelial cell differentiation and surfactant protein expression. Conditional RAGE over-expression was induced in mouse lung throughout gestation (embryonic day E0-18.5), as well as during the critical saccular period of development (E15.5-18.5), and analyses were conducted at E18.5. Histology revealed markedly less lung parenchyma beginning in the canalicular stage of lung development and continuing throughout the saccular period. We discovered consistently decreased expression of both NKX2.1 and FoxA2 in lungs from transgenic (TG) mice compared to littermate controls. We also observed diminished surfactant protein C in TG mice, suggesting possible hindered differentiation and/or proliferation of alveolar epithelial cells under the genetic control of these two critical transcription factors. These results demonstrate that RAGE must be specifically regulated during lung formation. Perturbation of epithelial cell differentiation culminating in respiratory distress and perinatal lethality may coincide with elevated RAGE expression in the lung parenchyma.","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":"11 3","pages":""},"PeriodicalIF":2.2,"publicationDate":"2023-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10366714/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10251557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evolutionary Change in Gut Specification in Caenorhabditis Centers on the GATA Factor ELT-3 in an Example of Developmental System Drift. 在发育系统漂移的一个例子中，隐杆线虫肠道规格的进化变化以GATA因子ELT-3为中心。

IF 2.7

Journal of Developmental Biology Pub Date : 2023-07-08 DOI: 10.3390/jdb11030032

Gina Broitman-Maduro, Morris F Maduro

{"title":"Evolutionary Change in Gut Specification in Caenorhabditis Centers on the GATA Factor ELT-3 in an Example of Developmental System Drift.","authors":"Gina Broitman-Maduro, Morris F Maduro","doi":"10.3390/jdb11030032","DOIUrl":"https://doi.org/10.3390/jdb11030032","url":null,"abstract":"Cells in a developing animal embryo become specified by the activation of cell-type-specific gene regulatory networks. The network that specifies the gut in the nematode Caenorhabditis elegans has been the subject of study for more than two decades. In this network, the maternal factors SKN-1/Nrf and POP-1/TCF activate a zygotic GATA factor cascade consisting of the regulators MED-1,2 → END-1,3 → ELT-2,7, leading to the specification of the gut in early embryos. Paradoxically, the MED, END, and ELT-7 regulators are present only in species closely related to C. elegans, raising the question of how the gut can be specified without them. Recent work found that ELT-3, a GATA factor without an endodermal role in C. elegans, acts in a simpler ELT-3 → ELT-2 network to specify gut in more distant species. The simpler ELT-3 → ELT-2 network may thus represent an ancestral pathway. In this review, we describe the elucidation of the gut specification network in C. elegans and related species and propose a model by which the more complex network might have formed. Because the evolution of this network occurred without a change in phenotype, it is an example of the phenomenon of Developmental System Drift.","PeriodicalId":15563,"journal":{"name":"Journal of Developmental Biology","volume":"11 3","pages":""},"PeriodicalIF":2.7,"publicationDate":"2023-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10366740/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10232972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0