Journal of biomedical materials research. Part B, Applied biomaterials最新文献_第8页

Use of Zebrafish (Danio rerio) for Biosafety Evaluation of Strontium Nanostructured Hydroxyapatite 利用斑马鱼（Danio rerio）评估锶纳米结构羟基磷灰石的生物安全性。

IF 3.2 4区医学

Journal of biomedical materials research. Part B, Applied biomaterials Pub Date : 2024-09-02 DOI: 10.1002/jbm.b.35478

Elisângela da Costa Silva, Augusto Monteiro de Souza, Alexandre Malta Rossi, Andrea Machado Costa, José Mauro Grangeiro, Ana Carolina Luchiari, Silvia Regina Batistuzzo de Medeiros

{"title":"Use of Zebrafish (Danio rerio) for Biosafety Evaluation of Strontium Nanostructured Hydroxyapatite","authors":"Elisângela da Costa Silva, Augusto Monteiro de Souza, Alexandre Malta Rossi, Andrea Machado Costa, José Mauro Grangeiro, Ana Carolina Luchiari, Silvia Regina Batistuzzo de Medeiros","doi":"10.1002/jbm.b.35478","DOIUrl":"10.1002/jbm.b.35478","url":null,"abstract":"<div>\u0000 \u0000 <p>Despite the numerous studies on biocompatibility with nano-biomaterials, the biological effects of strontium-substituted HA nanoparticles (nSrHA) need to be better understood. So, we conducted an embryotoxicity test using zebrafish (<i>Danio rerio</i>) according to the OECD 236 guideline, a model that represents a viable alternative that bridges the gap between in vitro and mammalian models. Zebrafish embryos were exposed for 120 h to microspheres containing nSrHA nanoparticles with low and high crystallinity, synthesized at temperatures of 5°C (nSrHA5) and 90°C (nSrHA90). We evaluated lethality, developmental parameters, and reactive oxygen species (ROS) production. The larval behavior was assessed at 168 hpf to determine if the biomaterials affected motor responses and anxiety-like behavior. The results showed that the survival rate decreased significantly for the nSrHA5 group (low crystalline particles), and an increase in ROS was also observed in this group. However, none of the biomaterials caused morphological changes indicative of toxicity during larval development. Additionally, the behavioral tests did not reveal any alterations in all experimental groups, indicating the absence of neurotoxic effects from exposure to the tested biomaterials. These findings provide valuable insights into the biosafety of modified HA-based nanostructured biomaterials, making them a promising strategy for bone tissue repair. As the use of hydroxyapatite-based biomaterials continues to grow, it is crucial to ensure rigorous control over the quality, reliability, and traceability of these materials.</p>\u0000 </div>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"112 9","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142119943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gd Added Mg Alloy for Biodegradable Implant Applications 用于生物降解植入物的钆添加镁合金

IF 3.2 4区医学

Journal of biomedical materials research. Part B, Applied biomaterials Pub Date : 2024-08-31 DOI: 10.1002/jbm.b.35474

Arun Kumar Surendran, Jithu Jayaraj, Rajinikanth Veerappan, Manoj Gupta, Srinivasan Amirthalingam, Raghu K Gopalan

{"title":"Gd Added Mg Alloy for Biodegradable Implant Applications","authors":"Arun Kumar Surendran, Jithu Jayaraj, Rajinikanth Veerappan, Manoj Gupta, Srinivasan Amirthalingam, Raghu K Gopalan","doi":"10.1002/jbm.b.35474","DOIUrl":"https://doi.org/10.1002/jbm.b.35474","url":null,"abstract":"<div>\u0000 \u0000 <p>Microstructure, mechanical, in vitro and in vivo behavior of extruded Mg alloys with varying Zn/Gd ratios, Mg-2Gd-2Zn-0.5Zr (Zn/Gd = 1), Mg-2Gd-6Zn-0.5Zr (Zn/Gd = 3), and Mg-10Gd-1Zn-0.5Zr (Zn/Gd = 0.1) were investigated. The results revealed that the major secondary phases such as W (Mg<sub>3</sub>Zn<sub>3</sub>Gd<sub>2</sub>), (Mg,Zn)<sub>3</sub>Gd, LPSO (Long period stacking order) and I (Mg<sub>3</sub>Zn<sub>6</sub>Gd) phase in alloys depended on Zn/Gd ratio. These second phases influenced the mechanical as well as biological characteristics of the alloys. Among studied alloys, Mg-10Gd-1Zn-0.5Zr alloy showed the highest yield strength and tensile strength of 270 (±9.29) and 330 MPa (±15.8), respectively, with a reasonably good elongation of 12% (±2.36). The presence of Gd<sub>2</sub>O<sub>3</sub> in the degradation film of Mg-10Gd-1Zn-0.5Zr enhanced the resistance offered by the film, which resulted in its lowest biodegradation, better viability, and cell proliferation under in vitro condition. The short term (subcutaneous implantation in rats for 1 month) in vivo studies showed that the alloy Mg-10Gd-1Zn-0.5Zr degraded at a rate of 0.35 mm/y (±0.02) and did not induce any toxicity to the vital organs.</p>\u0000 </div>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"112 9","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142100290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improving the Mechanical Properties of Glass Ionomer Cement With Nanocrystalline Cellulose From Rice Husk 用稻壳纳米纤维素改善玻璃离子聚合物水泥的力学性能

IF 3.2 4区医学

Journal of biomedical materials research. Part B, Applied biomaterials Pub Date : 2024-08-31 DOI: 10.1002/jbm.b.35472

Aninda Dwi Kartika Rini, Fifin Tresna Juwita, Riza Widyanti Bagjana, Sherly Octivany, Ryana Budi Purnama, Moch Saifur Rijal, Ahmad Miftahul Anwar, Bambang Sunendar Purwasasmita, Lia A. T. W. Asri

{"title":"Improving the Mechanical Properties of Glass Ionomer Cement With Nanocrystalline Cellulose From Rice Husk","authors":"Aninda Dwi Kartika Rini, Fifin Tresna Juwita, Riza Widyanti Bagjana, Sherly Octivany, Ryana Budi Purnama, Moch Saifur Rijal, Ahmad Miftahul Anwar, Bambang Sunendar Purwasasmita, Lia A. T. W. Asri","doi":"10.1002/jbm.b.35472","DOIUrl":"https://doi.org/10.1002/jbm.b.35472","url":null,"abstract":"<div>\u0000 \u0000 <p>This study aimed to evaluate the effect of incorporating nanocrystalline cellulose (NCC) sourced from rice husk on the mechanical properties of a commercial glass ionomer cement (GIC). NCC was isolated through acid hydrolysis, and its crystallinity, chemical structure, and morphology were characterized through x-ray diffractometry, Fourier-transform infrared spectroscopy, and transmission electron microscopy, respectively. Various concentrations of NCC (0%, 0.5%, 1%, and 1.5%) were added to reinforce the GIC matrix. Mechanical tests including compressive strength, flexural strength, hardness, and shear bond strength were conducted on the modified GIC samples. The addition of NCC resulted in increased hardness and shear bond strength values, with 1% NCC showing the highest values compared to other concentrations. However, there was no significant improvement observed in the compressive and flexural strength of the modified GIC. Failure mode test revealed a reduction in adhesive failure with the addition of NCC. Incorporating small amounts of NCC (0.5%–1%) suggests a promising and affordable modification of GIC restorative material using biomass residue, resulting in improved mechanical properties.</p>\u0000 </div>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"112 9","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142100289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel Honeycomb Nanoclay Frameworks With Hemostatic and Antibacterial Properties 具有止血和抗菌特性的新型蜂窝状纳米粘土框架

IF 3.2 4区医学

Journal of biomedical materials research. Part B, Applied biomaterials Pub Date : 2024-08-30 DOI: 10.1002/jbm.b.35477

Mélyssa Cambronel, Kan Wongkamhaeng, Christelle Blavignac, Christiane Forestier, Jean-Marie Nedelec, Isabelle Denry

{"title":"Novel Honeycomb Nanoclay Frameworks With Hemostatic and Antibacterial Properties","authors":"Mélyssa Cambronel, Kan Wongkamhaeng, Christelle Blavignac, Christiane Forestier, Jean-Marie Nedelec, Isabelle Denry","doi":"10.1002/jbm.b.35477","DOIUrl":"https://doi.org/10.1002/jbm.b.35477","url":null,"abstract":"<p>Our laboratory recently developed a new class of high surface area, honeycomb Nanoclay Microsphere Framework absorbents (NMFs) that prompt rapid hemostasis. In the present work, we propose a novel approach to develop antibacterial Topical Hemostatic Agents (THAs) by anchoring silver nanoparticles (AgNPs) onto NMFs. This combination was obtained by a chemical co-reduction approach, followed by freeze-processing, and was shown to ensure stability and on-site delivery of AgNPs, without altering the hemostatic properties of NMFs. Silver-loaded NMFs showed no change in their unique architecture and led to a 55% increase in clot strength, compared to standard control plasma or commercially available THA, and a significant decrease in mean fibrin fiber diameter. Silver nanoparticles were successfully released when solubilized and prevented the growth of both <i>Pseudomonas aeruginosa</i> and <i>Staphylococcus aureus</i> at concentrations of 22 and 30 ppm of silver released, respectively. Overall, cell mortality was between 9.1 ± 5.1% and 6.3 ± 3.2%, depending on AgNP concentration, confirming a low cytotoxicity. Silver-loaded nanoclay microsphere frameworks appear to constitute promising candidates as topical hemostatic agents for secondary management of hemostasis when infection control is needed.</p>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"112 9","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.b.35477","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142100438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluating the Feasibility of Short Dental Implants as Alternatives to Long Dental Implants in Mandibular Bone: A Finite Element Study 评估下颌骨中短牙种植体替代长牙种植体的可行性：有限元研究

IF 3.2 4区医学

Journal of biomedical materials research. Part B, Applied biomaterials Pub Date : 2024-08-30 DOI: 10.1002/jbm.b.35481

Prathamesh Deshmukh, Pankaj Dhatrak

{"title":"Evaluating the Feasibility of Short Dental Implants as Alternatives to Long Dental Implants in Mandibular Bone: A Finite Element Study","authors":"Prathamesh Deshmukh, Pankaj Dhatrak","doi":"10.1002/jbm.b.35481","DOIUrl":"https://doi.org/10.1002/jbm.b.35481","url":null,"abstract":"<div>\u0000 \u0000 <p>This study uses finite element analysis to investigate the potential application of shorter dental implants as a substitute for longer implants in the lower jaw (mandible). FEA allows the evaluation of the stress patterns around the implant-bone interface, a critical factor for successful osseointegration. Ten models were generated, encompassing five long (L1-L5) and five short implant models (S1-S5) with variations in diameter and length. Hypermesh software was utilized to meticulously prepare the FEA models, ensuring accurate mesh generation. The FEA simulations were conducted under four distinct loading scenarios (100 N occlusal load, 40 N lateral load, 100 N oblique at 30°, and 100 N oblique at 45°) to realistically mimic the forces exerted during biting, using an ABAQUS CAE solver. The results revealed that the von Mises stress generated within the short implant models was demonstrably lower compared to their long implants. Additionally, a significant drop in stress was observed with increasing the diameter of the short implants, to a certain diameter range. These findings suggest the potential for successful substitution of long implant model L4 with short implant model S4 due to the demonstrably lower stress values achieved. Furthermore, the data indicates the possibility of utilizing short implant models S3 and S5 as alternatives to long implant models L3 and L5, respectively. These observations hold significant promise for evaluating the feasibility of replacing long implants with shorter variants, potentially leading to a reduction in implant-related failures.</p>\u0000 </div>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"112 9","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142100439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Versatile cell cultivation on injectable poly(butylene adipate-co-terephthalate) microcarriers: Impact of surface properties across different cell types 注射用聚（丁烯-己二酸共对苯二甲酸酯）微载体上的多功能细胞培养：不同细胞类型表面特性的影响。

IF 3.2 4区医学

Journal of biomedical materials research. Part B, Applied biomaterials Pub Date : 2024-08-28 DOI: 10.1002/jbm.b.35464

Sena Koç, Menemşe Gümüşderelioğlu

{"title":"Versatile cell cultivation on injectable poly(butylene adipate-co-terephthalate) microcarriers: Impact of surface properties across different cell types","authors":"Sena Koç, Menemşe Gümüşderelioğlu","doi":"10.1002/jbm.b.35464","DOIUrl":"10.1002/jbm.b.35464","url":null,"abstract":"<p>Injectable cell therapies offer several advantages compared with traditional open surgery, including less trauma to the patient, shorter recovery time, and lower risk of infection. However, a significant problem is the difficulty in developing effective cell delivery carriers that are cyto-compatible and maintain cell viability both during and after injection. In the presented study, it was aimed to develop poly(butylene adipate-co-terephthalate) (PBAT) microcarriers using the emulsion preparation-solvent evaporation technique. The optimized diameter of the PBAT microcarriers was determined as 104 ± 15 μm at 700 rpm and there would be no blockage after injection due to the nonswelling feature of microcarriers. Furthermore, the cellular activities of PBAT microcarriers were evaluated in static culture for 7 days using L929 mouse fibroblasts, MC3T3-E1 mouse pre-osteoblasts, and rat adipose-derived mesenchymal cells (AdMSCs). 3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide results and Sscanning electron microscope images showed that PBAT microcarriers increased the adhesion and proliferation properties of pre-osteoblasts and stem cells, while L929 fibroblasts formed aggregates by adhering to certain regions of the microcarrier surface and did not spread on the surface. These results emphasize that PBAT microcarriers can be used as injectable carriers, especially in stem cell therapies, but their surface properties need to be modified for some cells.</p>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"112 9","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.b.35464","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142080429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Interleukin-4-Loaded Gelatin Methacryloyl Hydrogel Promotes Subcutaneous Chondrogenesis of Engineered Auricular Cartilage in a Rabbit Model 白细胞介素-4负载明胶甲基丙烯酰水凝胶促进兔模型中耳廓软骨的皮下软骨生成

IF 3.2 4区医学

Journal of biomedical materials research. Part B, Applied biomaterials Pub Date : 2024-08-28 DOI: 10.1002/jbm.b.35473

Jinqiao Li, Xia Liu, Haiyue Jiang, Mingyong Yang

{"title":"Interleukin-4-Loaded Gelatin Methacryloyl Hydrogel Promotes Subcutaneous Chondrogenesis of Engineered Auricular Cartilage in a Rabbit Model","authors":"Jinqiao Li, Xia Liu, Haiyue Jiang, Mingyong Yang","doi":"10.1002/jbm.b.35473","DOIUrl":"https://doi.org/10.1002/jbm.b.35473","url":null,"abstract":"<div>\u0000 \u0000 <p>Tissue engineering technology offers a promising solution for ear reconstruction; however, it faces the challenge of foreign body reaction and neocartilage malformation. This study investigates the impact of interleukin-4 (IL-4), an anti-inflammatory factor, on cartilage regeneration of hydrogel encapsulating autologous auricular chondrocytes in a rabbit subcutaneous environment. Initially, we assessed the influence of IL-4 on chondrocyte proliferation and determined the appropriate concentration using the CCK-8 test in vitro. Subsequently, we loaded IL-4 into gelatin methacryloyl (GelMA) hydrogel containing chondrocytes and measured its release profile through ELISA. The constructs were then implanted autologously into rabbits' subcutis, and after 3, 7, 14, and 28 days, cartilage matrix formation was evaluated by histological examinations, and gene expression levels were detected by qRT-PCR. Results demonstrated that IL-4 promotes chondrocyte proliferation in vitro, and maximum release from constructs occurred during the first week. In the rabbit subcutaneous implantation model, IL-4-loaded constructs (20 ng/mL) maintained a superior chondrocytic phenotype compared to controls with increased expression of anti-inflammatory factors. These findings highlight IL-4 as a potential strategy for promoting chondrogenesis in a subcutaneous environment and improving ear reconstruction.</p>\u0000 </div>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"112 9","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142089899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of pH on hydroxyapatite coatings obtained by spray pyrolysis and their use as matrices for antibiotic adsorption by spin coating and release properties pH 值对通过喷雾热解获得的羟基磷灰石涂层的影响，以及将其用作通过旋涂吸附抗生素的基质和释放特性。

IF 3.2 4区医学

Journal of biomedical materials research. Part B, Applied biomaterials Pub Date : 2024-08-28 DOI: 10.1002/jbm.b.35470

Iván D. Rojas-Montoya, Luis Sergio Escamilla-Atriano, Antonio Reina, Sandra Ramírez-Rave, Perla García-Guzmán, Ma Josefa Bernad-Bernad, Jesús Gracia-Mora

{"title":"Effect of pH on hydroxyapatite coatings obtained by spray pyrolysis and their use as matrices for antibiotic adsorption by spin coating and release properties","authors":"Iván D. Rojas-Montoya, Luis Sergio Escamilla-Atriano, Antonio Reina, Sandra Ramírez-Rave, Perla García-Guzmán, Ma Josefa Bernad-Bernad, Jesús Gracia-Mora","doi":"10.1002/jbm.b.35470","DOIUrl":"10.1002/jbm.b.35470","url":null,"abstract":"<p>Calcium phosphate materials, particularly hydroxyapatite (HA), are extensively used in biomedical applications because of their prominence as primary inorganic constituents of human hard tissues. This study investigates the synthesis of HA coatings via spray pyrolysis using various precursors, including HA derived from bovine bone. The effects of pH on the formation and properties of HA coatings were systematically examined. Samples exposed to acidic conditions or left without pH adjustment led to the formation of HA, contrasting with the outcomes observed through dissolution methods. Different characterization techniques, such as scanning electron microscopy (SEM), transmission electron microscopy (TEM), and X-ray diffraction (XRD), were employed to evaluate the quality and crystallinity of the coatings. Among the samples, those exhibiting superior crystallinity and nanostructured features, including bovine HA, were selected for further surface functionalization with the antibiotic enrofloxacin using spin coating. As expected, the antibiotic loading on each material's surface depended on the amount of HA deposited on the substrate. However, the desorption results indicated that, in all cases, desorption persisted beyond 38 h, implying that HA-loaded matrices could be effective systems for controlled and prolonged drug release, which could be useful in dental or orthopedic implants for inhibiting the growth of bacterial biofilms.</p>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"112 9","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.b.35470","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142080428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

3D-printed silicate porous bioceramics promoted the polarization of M2-macrophages that enhanced the angiogenesis in bone regeneration 三维打印硅酸盐多孔生物陶瓷促进了M2-巨噬细胞的极化，从而增强了骨再生过程中的血管生成

IF 3.2 4区医学

Journal of biomedical materials research. Part B, Applied biomaterials Pub Date : 2024-08-28 DOI: 10.1002/jbm.b.35469

Chengwu Zang, Min Che, Hang Xian, Xin Xiao, Tengfei Li, Yongxiang Chen, Yaxiong Liu, Rui Cong

{"title":"3D-printed silicate porous bioceramics promoted the polarization of M2-macrophages that enhanced the angiogenesis in bone regeneration","authors":"Chengwu Zang, Min Che, Hang Xian, Xin Xiao, Tengfei Li, Yongxiang Chen, Yaxiong Liu, Rui Cong","doi":"10.1002/jbm.b.35469","DOIUrl":"https://doi.org/10.1002/jbm.b.35469","url":null,"abstract":"<p>The failure of bone regeneration has been considered as a serious problem that troubling patients for decades, most of which was resulted by the poor angiogenesis and chronic inflammation after surgery. Among multiple materials applied in the repair of bone defect, silicate bioceramics attracted researchers because of its excellent bioactivity. The purpose of this study was to detect the effect of specific bioactive glass ceramic (AP40, based on crystalline phases of apatite and wollastonite) on angiogenesis and the subsequent bone growth through the modulation of macrophages. Two groups were included in this study: control group (macrophages without any stimulation, denominated as Control) and AP40 group (macrophages incubated on AP40). This study investigated the effect of AP40 on macrophages polarization (RAW264.7) and angiogenesis in vitro and in vivo. Additionally, the changes of angiogenic ability regulated by macrophages were explored. AP40 showed excellent angiogenesis potential and the expression of CD31 was promoted through the modulation of macrophages toward M2 subtype. Additionally, the macrophages incubated on AP40 synthesized more PDGF-BB comparing to macrophages without any stimulation, which contributed to the improved angiogenetic ability of human umbilical vein endothelial cells (HUVECs). Results of in vivo studies indicated increased bone ingrowth along the implants, which indicated the potential of bioceramics for bone defect repair clinically.</p>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"112 9","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142099961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Carbon fiber felt scaffold from Brazilian textile PAN fiber for regeneration of critical size bone defects in rats: A histomorphometric and microCT study 利用巴西纺织品 PAN 纤维制成的碳纤维毡支架用于大鼠临界尺寸骨缺损的再生：组织形态计量学和显微 CT 研究。

IF 3.2 4区医学

Journal of biomedical materials research. Part B, Applied biomaterials Pub Date : 2024-08-23 DOI: 10.1002/jbm.b.35467

Kauê Alberto Pereira, Letícia Cavassini Torquato, Clarissa Carvalho Martins Maciel, Camilla Magnoni Moretto Nunes, Ludmilla Oliveira Mantovani, Nátaly Domingues Almeida, Sergio Lucio Pereira Castro Lopes, Luana Marotta Reis de Vasconcellos, Maria Aparecida Neves Jardini, Jossano Saldanha Marcuzzo, Andrea Carvalho De Marco

{"title":"Carbon fiber felt scaffold from Brazilian textile PAN fiber for regeneration of critical size bone defects in rats: A histomorphometric and microCT study","authors":"Kauê Alberto Pereira, Letícia Cavassini Torquato, Clarissa Carvalho Martins Maciel, Camilla Magnoni Moretto Nunes, Ludmilla Oliveira Mantovani, Nátaly Domingues Almeida, Sergio Lucio Pereira Castro Lopes, Luana Marotta Reis de Vasconcellos, Maria Aparecida Neves Jardini, Jossano Saldanha Marcuzzo, Andrea Carvalho De Marco","doi":"10.1002/jbm.b.35467","DOIUrl":"10.1002/jbm.b.35467","url":null,"abstract":"<p>The objective of the present study was to evaluate the carbon fiber obtained from textile PAN fiber, in its different forms, as a potential scaffolds synthetic bone. Thirty-four adult rats were used (<i>Rattus norvegicus</i>, albinus variation), two critical sized bone defects were made that were 5 mm in diameter. Twenty-four animals were randomly divided into four groups: control (C)—bone defect + blood clot, non-activated carbon fiber felt (NACFF)—bone defect + NACFF, activated carbon fiber felt (ACFF)—bone defect + ACFF, and silver activated carbon fiber felt (Ag-ACFF)—bone defect + Ag-ACFF, and was observed by 15 and 60 days for histomorphometric, three-dimensional computerized microtomography (microCT) and mineral apposition analysis. On histomorphometric and microCT analyses, NACFF were associated with higher proportion of neoformed bone and maintenance of bone structure. On fluorochrome bone label, there was no differences between the groups. NACFF has shown to be a promising synthetic material as a scaffold for bone regeneration.</p>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"112 9","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142046667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0