Journal of biomedical materials research. Part B, Applied biomaterials最新文献

{"title":"Unveiling the Synergistic Influence of TiO2 and Chitosan-Based Hydrogel in Precision Surface Co-Modification for Superior Pure Titanium Implant Performance","authors":"Shaahin Mohammadzadeh Asl,&nbsp;Babak Akbari,&nbsp;Marjan Bahraminasab,&nbsp;Samaneh Arab","doi":"10.1002/jbm.b.35605","DOIUrl":"https://doi.org/10.1002/jbm.b.35605","url":null,"abstract":"<div>\u0000 \u0000 <p>Bacterial infections are the primary cause of surgical failures associated with orthopedic implants. One promising avenue to address this challenge and capitalize on the effectiveness of antibiotic administration involves utilizing titania nanotubes (TNTs) loaded with vancomycin (Van). Electrochemical anodization offers a contemporary approach for producing Titania-NTs with applications in localized vancomycin delivery. However, the regulation of the drug release mechanism from these delivery systems is complex. This research delves into the controlled release of vancomycin from Titania-NT surfaces enriched with drug/polymer through physical absorption. The loading and in vitro releases of Van were analyzed using the Korsmeyer–Peppas kinetic model (R² = 0.99), which indicated non-Fickian diffusion (case II/I, <i>n</i> = 0.41). Antibacterial activity was tested against <i>Staphylococcus aureus</i> and <i>Pseudomonas aeruginosa</i> through turbidity measurements, CFU counts, and agar disc diffusion assays. Protein adsorption on titanium surfaces was evaluated, and gene expression analysis was performed to assess osteogenic markers (ALP and OCN). Vancomycin-loaded TNTs (11 μM) stimulated MC3T3-E1 cell proliferation, demonstrating enhanced cell viability. The presence of chitosan effectively controlled the Van delivery process. The morphology of anodized Ti surfaces played a key role in inhibiting bacterial growth. Results demonstrated reduced bacterial growth and significant protein adsorption on modified surfaces. Chitosan-loaded TNTs significantly upregulated osteogenic markers ALP and OCN, with T60-van/chit-20 showing the highest expression levels, enhancing osteogenic differentiation and bone integration.</p>\u0000 </div>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"113 8","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144673056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibacterial and Regenerative Potential of Zinc Oxide Nanoparticles and Carboxymethyl Cellulose for Infected Wound Healing in Rats","authors":"Nermeen - Shereef,&nbsp;Marwa Abass,&nbsp;Emad Tolba,&nbsp;Wafaa Ragab,&nbsp;Awad Rizk,&nbsp;Gamal Karrouf","doi":"10.1002/jbm.b.35607","DOIUrl":"https://doi.org/10.1002/jbm.b.35607","url":null,"abstract":"<div>\u0000 \u0000 <p>Infection remains a critical challenge in wound healing, often delaying recovery and complicating the case. The widespread misuse of antibiotics has further exacerbated this issue by driving antibiotic resistance, creating an urgent need for alternative therapeutic strategies. This study investigates the potential effects of Zinc Oxide Nanoparticles (ZnONPs) combined with Carboxymethyl Cellulose (CMC) as a solution to overcome these barriers. By employing a comprehensive approach, the effect of these materials was evaluated through histopathological, immunohistochemical, and bacteriological analyses, alongside macroscopic observations, which included body weight, wound size, and contraction assessment. Fifty-four male adult Albino Wistar rats were used in this study. A full-thickness circular <i>Staphylococcus aureus</i>-infected skin wound of a 23 mm diameter was induced in all rats. After 3 days, these rats were divided into 3 groups according to the treatment protocol: the infected group (treatment with normal saline 0.9%), the CMC group (treatment with CMC alone), and the CMC/ZnONPs group (treatment with CMC/ZnONPs gel). Rats were then euthanized, and samples were collected for histopathology and immunohistochemistry on days 7, 14, and 21 postoperatively. Swabs were taken from the wounds for bacterial examination on the 2nd, 7th, 14th, and 21st postoperatively. Although the CMC alone enhanced the wound healing, the combination of the CMC and the ZnONPs demonstrates a significantly superior effect by enhancing angiogenesis, reducing inflammation, and accelerating re-epithelialization. Additionally, for CMC to achieve an effective antibacterial effect, it must be applied four times daily, whereas the CMC/ZnONPs gel requires only twice-daily application. Therefore, the incorporation of ZnONPs appears essential for CMC to exert effective antibacterial activity.</p>\u0000 </div>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"113 8","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144673195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aligned Collagen Sponges Loaded With Myogenic Factors to Enhance Skeletal Muscle Tissue Regeneration","authors":"Natalie G. Kozan,&nbsp;Sean Caswell,&nbsp;Shreyas Bolla,&nbsp;Jonathan M. Grasman","doi":"10.1002/jbm.b.35619","DOIUrl":"https://doi.org/10.1002/jbm.b.35619","url":null,"abstract":"<div>\u0000 \u0000 <p>Volumetric muscle loss (VML) is an injury which leads to debilitating loss of functionality of a muscle. Autologous tissue grafts are used as a standard treatment; however, these grafts often result in complications. Current scaffolds are limited in their ability to restore functionality of the injured muscle, which may be due to lack of cell recruitment to the scaffold and/or lack of sufficient myofiber formation. Our approach involves the controlled release of insulin-like growth factor-1 (IGF-1) from a biopolymer scaffold, as IGF-1 enhances both the proliferation and differentiation of myoblasts, which we hypothesize will enhance regeneration after VML. In this study, to facilitate a more controlled release of IGF-1, we added IGF-binding protein 5 (IGFBP-5) to anisotropic collagen sponges with finely tuned pore sizes via heparin conjugation to stabilize the IGFBP-5/IGF-1 complex. Scaffolds containing heparin, IGFBP-5, and IGF-1 induced the highest level of myofiber formation for up to 4 weeks, suggesting this scaffold system supported the sustained release of active IGF-1. Future studies will be used to implant these scaffolds into mouse models of VML and determine their effects on regeneration in vivo as well as on restoration of force production of the muscle.</p>\u0000 </div>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"113 8","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144673052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hydrothermal Synthesis of Chlorogenic Acid-Functionalized Ag Nanoparticles and Their Antibacterial Activity","authors":"Shengkai Liu,&nbsp;Hongji Wang,&nbsp;Siqi He,&nbsp;Xiaoyu Li,&nbsp;Liying Cui,&nbsp;Zhongkai Liu,&nbsp;Xiaoshuai Wang,&nbsp;Mengqi Liu,&nbsp;Yujie Fu,&nbsp;Zhiguo Liu","doi":"10.1002/jbm.b.35616","DOIUrl":"https://doi.org/10.1002/jbm.b.35616","url":null,"abstract":"<div>\u0000 \u0000 <p>In this study, chlorogenic acid (CGA), a natural organic acid, was employed as both a reducing and stabilizing agent to synthesize chlorogenic acid-functionalized silver nanoparticles (CGA-AgNPs). The synthesis conditions of CGA-AgNPs, including reactant ratio, pH, temperature, and reaction time, were optimized. The synthesized CGA-functionalized AgNPs were characterized by ultraviolet–visible (UV–Vis) spectroscopy, X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), and transmission electron microscopy (TEM). TEM imaging results indicated that CGA-AgNPs were spherical nanoparticles with an average diameter of 36.70 ± 0.65 nm. FTIR spectroscopy results confirmed that CGA was covered on the surface of AgNPs. XPS results indicated that the surface of nanoparticles was made up of Ag atoms. The inhibitory activity of CGA-AgNPs against <i>Candida albicans</i> (<i>C. albicans</i>), <i>Acinetobacter baumanniiand</i> (<i>A. baumannii</i>), and <i>Staphylococcus aureus</i> (<i>S. aureus</i>) was measured and evaluated by the inhibition circle method and the minimum inhibitory concentration. The synthesized CGA-AgNPs under optimal conditions indicated good antibacterial activity against <i>C. albicans, A. baumannii</i>, and <i>S. aureus</i> with MIC values of 36.95 ± 0.22 μg/mL and MBC values of 73.90 ± 0.22 μg/mL. In summary, this study provides a facile and green synthesis method to prepare CGA-AgNPs with excellent antibacterial activities.</p>\u0000 </div>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"113 8","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144673197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive Prediction of the Relative Modulus of Strut-Based Gyroid Lattice Structures Employing an ML-Based Surrogate Model","authors":"Naufal Muhammad Judawisastra,&nbsp;Satrio Wicaksono,&nbsp;Yohanes Bimo Dwianto,&nbsp;Andi Isra Mahyuddin,&nbsp;Tatacipta Dirgantara,&nbsp;Lavi Rizki Zuhal","doi":"10.1002/jbm.b.35613","DOIUrl":"https://doi.org/10.1002/jbm.b.35613","url":null,"abstract":"<div>\u0000 \u0000 <p>Due to its porous structure, tunable properties, and nearly isotropic characteristics, the Gyroid Lattice Structure (GLS) is widely utilized in orthopedic implant applications. To effectively reduce stress shielding and enhance implant longevity, it is essential to accurately predict the GLS's elastic moduli across a broad range of relative densities for various material selections. This study conducted a comprehensive finite element analysis of strut-based GLS models, considering unit cell arrangements, a wider range of relative densities, and variations in lattice orientations to predict its relative elastic moduli. GLS models with relative densities of 10%, 30%, 50%, and 75% were experimentally tested and numerically analyzed to capture properties across a broader density range. The well-known Gibson-Ashby model and a machine learning (ML)-based surrogate model employing Gaussian Process Regression were developed to extend predictions across the full-density spectrum. The results showed that different relative densities required varying numbers of unit cells to achieve elastic modulus convergence. The improved Gibson-Ashby model provided closer predictions to experiments over a wider density range but struggled to fully capture behavior at high relative densities near bulk material properties. In contrast, the ML-based surrogate model accurately predicts elastic moduli across the entire relative density range. Compared to experimental results, this approach demonstrates greater accuracy in predicting the elastic modulus of GLS, with reduced error compared to other methods. These findings are particularly valuable for optimizing implant and scaffold designs, as accurate modulus predictions contribute to improved performance and longevity, helping to mitigate the stress-shielding effect.</p>\u0000 </div>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"113 8","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144673196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ellagic Acid-Zinc Oxide Nanoparticles Show Antioxidant, Antibacterial Activities and Induce Apoptosis in Lung Cancer A549 Cells","authors":"Farkhondeh Ghoncheh-Moghadam,&nbsp;Ahmad Asoodeh,&nbsp;Abbas Vaezi-Kakhki","doi":"10.1002/jbm.b.35604","DOIUrl":"https://doi.org/10.1002/jbm.b.35604","url":null,"abstract":"<div>\u0000 \u0000 <p>Cancer is one of the biggest causes of death in today's world. In the field of cancer treatment, the use of natural compounds has attracted the attention of researchers. A variety of nanoparticles, including zinc oxide nanoparticles, represent a widely utilized therapeutic approach today due to their exceptional biocompatibility and safety profile. In this study, ellagic acid, which is a natural polyphenolic compound, was used together with synthesized zinc oxide nanoparticles. The loading of ellagic acid in zinc oxide nanoparticles was confirmed by FT-IR and XRD methods. Examination of the viability of A549 cancer cells showed that the cytotoxicity of nano drug Ellagic acid-Zno nanoparticle (EAN) is more than that of ellagic acid alone. The percentage of reactive oxygen species (ROS) produced by EAN was higher than that of ellagic acid. Apoptosis was observed in the treated cells by acridine-orange-ethidium-bromide staining. Investigating the cell migration of EAN caused a decrease in cell migration. Based on the research, suggested that EAN can be used as multifunctional nanomedicine nanoparticles.</p>\u0000 </div>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"113 8","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144666443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Surface Modification of Stainless Steel 316L by Electrophoretic Deposition of Hydroxyapatite for Enhanced Biocompatibility","authors":"Sehrish Mukhtar,&nbsp;Muhammad Hamza,&nbsp;Asima Tayyeb,&nbsp;Isbah Ashfaq,&nbsp;Faraz Hussain,&nbsp;Muhammad Ishtiaq,&nbsp;Sung-Gyu Kang","doi":"10.1002/jbm.b.35618","DOIUrl":"https://doi.org/10.1002/jbm.b.35618","url":null,"abstract":"<div>\u0000 \u0000 <p>Stainless Steel 316L (SS316L) was coated with hydroxyapatite (HA) by electrophoretic deposition method. The uncoated and two coated samples (at 20 V for 20 min, and 30 V for 30 min) were compared to investigate the coating effect on hardness, corrosion resistance, and biocompatibility. The microstructures of uncoated and HA-coated samples were evaluated by scanning electron microscope (SEM) and energy dispersive X-ray diffraction (EDX). Scratch test and micro Vickers hardness test were used to evaluate the adhesion and hardness of HA-coated samples. The electrochemical and in vitro biocompatibility analyses were carried out using Potentiostat and Cytotoxicity Assay. Cross-sectional SEM images showed uniform, homogenous, and crack-free HA coating on SS316L with a thickness of 45 μm at 20 V and 55 μm at 30 V. EDX analysis revealed the presence of calcium, phosphorous, and oxygen elements in the coated SS316L surface. The average surface hardness was 245 HV and 289 HV for coated samples at 20 and 30 V respectively. The scratch test showed better adhesive strength of coating, and electrochemical testing in Ringer Lactate solution showed improved corrosion resistance for the coated sample at 30 V as compared to 20 V and the uncoated sample. The cytotoxicity assay confirmed improved cell viability and growth on SS316L samples coated with HA at 30 V as compared to 20 V and uncoated samples. SS316L with HA coating at 30 V, with its better mechanical properties, low corrosion resistance, and good biocompatibility, represents a potential biomaterial candidate for biomedical applications.</p>\u0000 </div>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"113 7","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144606462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Peri-Implant Bone Repair of Commercially Pure Titanium or Titanium-Aluminum-Vanadium Alloy Implants With or Without Laser-Modified Surface","authors":"Laís Kawamata de Jesus,&nbsp;Henrique Hadad,&nbsp;Luis Fernando Alves da Silva,&nbsp;Letícia Gabriella de Souza Rodrigues,&nbsp;Ana Flávia Piquera Santos,&nbsp;Thallita Pereira Queiroz,&nbsp;Rodolfo Debone Piazza,&nbsp;Fernando Pozzi Semeghini Guastaldi,&nbsp;Pier Paolo Poli,&nbsp;Carlo Maiorana,&nbsp;Antonio Carlos Guastaldi,&nbsp;Roberta Okamoto,&nbsp;Francisley Ávila Souza","doi":"10.1002/jbm.b.35610","DOIUrl":"https://doi.org/10.1002/jbm.b.35610","url":null,"abstract":"<div>\u0000 \u0000 <p>To evaluate the peri-implant bone repair of implants manufactured from commercially pure titanium <i>(cp-Ti)</i> or titanium-aluminum-vanadium alloy <i>(Ti-6Al-4 V)</i> with machined <i>(MS)</i> or laser-modified <i>(LS)</i> surfaces. Implants (<i>n</i> = 192) were installed in the tibias of 96 rats and divided according to the implant installed: (1) cpMS: <i>cp-Ti</i> with an MS surface, (2) cpLS: <i>cp-Ti</i> with an LS surface, (3) alloyMS: <i>Ti-6Al-4 V</i> with an MS surface, and (4) alloyLS: <i>Ti-6Al-4 V</i> with an LS surface. Scanning electron microscopy-energy dispersive X-ray (SEM–EDX) analysis was performed prior to implant placement. At 14, 21, and 42 days, biomechanical analysis, SEM–EDX of the removed implants, and measurement of the percentage of bone-implant contact (%BIC) and neoformed bone area (%NBA) were performed. At 42 days, the mineral apposition rate (MAR) and bone dynamics were analyzed. At 14 and 21 days, the alloyLS group showed higher removal torque values than cpLS (<i>p</i> &lt; 0.05). For SEM–EDX, the cpLS and alloyLS groups showed surfaces covered by bone tissue and higher peaks of calcium and phosphorus. No statistical differences were observed for %BIC, and at 14 days, a higher %NBA was found for alloyLS than cpLS (<i>p</i> &lt; 0.05). At 42 days, in bone dynamics, alloyLS showed a higher amount of calcein compared to cpLS (<i>p</i> &lt; 0.05), and higher MAR values were observed for cpLS (<i>p</i> &lt; 0.05). The alloyLS group promoted accelerated peri-implant bone repair at 14 and 21 days. However, at 42 days, cpLS surpassed the values of removal torque, %BIC, %NBA, bone dynamics, and MAR.</p>\u0000 </div>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"113 7","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144558077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nitinol in Orthopedic Applications: Clinical Insights, Performance Challenges, and Future Directions","authors":"Niyou Wang,&nbsp;Veluru Jagadeesh Babu,&nbsp;Namith Rangaswamy,&nbsp;Si Jian Hui,&nbsp;Joshua K,&nbsp;James Thomas Patrick Decourcy Hallinan,&nbsp;Senthil Kumar A,&nbsp;Naresh Kumar","doi":"10.1002/jbm.b.35615","DOIUrl":"https://doi.org/10.1002/jbm.b.35615","url":null,"abstract":"<p>The increasing prevalence of spinal disorders has spurred continuous innovation in implant design and biomaterials. Among emerging options, Nitinol has gained significant interest due to its unique combination of shape memory effect, superelasticity, and mechanical compatibility with bone tissue. These characteristics make it a promising candidate for spinal implants that support minimally invasive surgery and motion preservation. This review offers a comprehensive overview of Nitinol's clinical applications in orthopedics, with a particular focus on spinal procedures. It explores key material properties, device types, and performance benefits, including reduced stress shielding and improved implant-tissue integration. A wide range of Nitinol-based devices such as screws, plates and rods, nails, artificial discs, staples, and dynamic systems are discussed alongside clinical outcomes and case studies. Emerging directions such as additive manufacturing for patient-specific implants, and smart Nitinol systems with integrated sensing or actuation capabilities are discussed. In addition, challenges in thermal control, manufacturing reproducibility, and evolving regulatory standards are addressed. By synthesizing current advancements and unmet needs, this review highlights the clinical potential of Nitinol implants and outlines future directions for their safe, effective, and scalable integration into orthopedic practice.</p>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"113 7","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.b.35615","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144536967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibody Mediated Osseous Regeneration (AMOR) in Conjunction With Decortication in Rabbit Calvaria Model","authors":"Seiko Min,&nbsp;Thomas Nguyen,&nbsp;Keitetsu Kure,&nbsp;Akira Hasuike,&nbsp;Shuichi Sato,&nbsp;Homayoun Zadeh","doi":"10.1002/jbm.b.35609","DOIUrl":"https://doi.org/10.1002/jbm.b.35609","url":null,"abstract":"<p>This study aimed to assess the osteogenic potential of chimeric monoclonal antibodies targeting BMP-2 (Anti-BMP-2 mAb) in conjunction with decortication in a rabbit calvaria model using titanium domes. Anti-BMP-2 mAb and an isotype matched control mAb were immobilized on anorganic bovine bone mineral with 10% collagen scaffold (ABBM-C) and placed within titanium domes positioned over rabbit calvaria (<i>n</i> = 6), with surfaces either decorticated or intact. Four groups were defined: (1) anti-BMP-2 mAb + decortication (AMD); (2) anti-BMP-2 mAb + no-decortication (AM); (3) isotype matched control mAb + decortication (IsoD); (4) isotype matched control mAb + no-decortication (Iso). After 3 months, rabbits were euthanized, and histologic observation and histomorphometric analysis were performed. ANOVA assessed group differences. Vital bone volume for the groups was: (1) 34.2% ± 7.8%; (2) 22.3% ± 4.1%, (<i>p</i> &lt; 0.02 for groups AMD vs. AM); (3) 23.4% ± 6.2%, (<i>p</i> &lt; 0.04 for AMD vs. IsoD); and (4) 17.5% ± 5.9%, (<i>p</i> &lt; 0.003 for groups AMD vs. Iso). All groups exhibited decreased bone formation further from the calvaria bone, yet the Anti-BMP-2 mAb + decortication group maintained 30%–40% vital bone formation within 3 mm from the calvaria bone. This study highlights the superior osteogenic efficacy of Anti-BMP-2 mAb with decortication. Decortication notably enhanced the osteogenic potential of scaffolds functionalized with Anti-BMP-2 mAb.</p>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"113 7","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.b.35609","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144536898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}