Journal of biomedical materials research. Part B, Applied biomaterials最新文献

{"title":"Characterization of Extracellular Matrix Derived From Porcine Organs Decellularized Using Different Methods","authors":"Vignesh Dhandapani,&nbsp;Pakindame Boabekoa,&nbsp;Martin Borduas,&nbsp;Patrick Vermette","doi":"10.1002/jbm.b.35498","DOIUrl":"10.1002/jbm.b.35498","url":null,"abstract":"<p>Regenerative medicine has extended the capacity of medicine to a point where tissues and organs could potentially be manufactured. This could resolve issues associated with organ transplantation. The extracellular matrix (ECM) provides a supportive scaffold and biochemical cues allowing cells to attach, proliferate, and differentiate. The ECM is composed of different fibrous proteins and proteoglycans. The extensive value of ECM lies in its dynamic microenvironment that aids in cell proliferation, differentiation, and regulation of intercellular communication. In this study, four methods were applied to decellularize porcine organs. The ECMs were characterized by histological methods illustrating the absence of nuclei and the presence of glycosaminoglycans (GAGs) and collagen. Hematoxylin and eosin analysis of native pancreas revealed necrosis by auto-digestion, also supported by a reduced dsDNA content, and could have led to the destruction of Type IV collagen, laminins, and other proteins in the resulting ECMs, as confirmed by mass spectrometry. DNA quantification of ECM revealed residual dsDNA contents lower than those of the native organs. Bicinchoninic acid (BCA) assay showed a difference in protein content between organs. Mass spectrometry coupled with proteomic analysis highlighted a significant difference in the protein composition. The number of different proteins, in some cases with more than 2700, in the produced ECM depended on the applied decellularization technique. Polarization microscopy indicated differences in the orientation of collagen fibers. This study provides a multimodal approach to characterize ECMs produced using different decellularization techniques, aiding in finding a balance between maintaining the ultrastructure and composition of ECM, while removing cellular components.</p>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"112 11","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.b.35498","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142581160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alginate-HEMA Hydrogels as Promising Biomaterials for Bone Regeneration: In Vitro and In Vivo Studies","authors":"M. L. Torres,&nbsp;A. Hurtado Cuba,&nbsp;T. G. Oberti,&nbsp;J. M. Fernandez","doi":"10.1002/jbm.b.35493","DOIUrl":"10.1002/jbm.b.35493","url":null,"abstract":"<div>\u0000 \u0000 <p>In the present work, the osteogenic and angiogenic properties of, previously developed, semi-interpenetrated HEMA-EGDMA polymeric networks (sIPN) with and without alginate with application in bone tissue engineering (BTE) were studied. In vitro characterization studies were performed using rat bone marrow progenitor cells (BMPCs), EA.hy926 endothelial cells, and rat vascular smooth muscle cells (VSMCs). Based on the in vitro results of both this work and previous ones, the hydrogels were selected to carry out in vivo studies to find out their capacity as a biomaterial using a bone regeneration model. Our results indicate that the incorporation of alginate into the HEMA-EGDMA polymeric network promotes osteogenic and angiogenic capacity in cell cultures of BMPCs and both EA.hy926 and VSMCs, respectively, and also increases bone formation and vascular structures in in vivo studies, demonstrating its potential use as a biomaterial in BTE.</p>\u0000 </div>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"112 11","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142545693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Implementing Chicken Chorioallantoic Membrane (CAM) Assays for Validating Biomaterials in Tissue Engineering: Rationale and Methods","authors":"Mélanie Dhayer,&nbsp;Amélia Jordao,&nbsp;Salim Dekiouk,&nbsp;Damien Cleret,&nbsp;Nicolas Germain,&nbsp;Philippe Marchetti","doi":"10.1002/jbm.b.35496","DOIUrl":"10.1002/jbm.b.35496","url":null,"abstract":"<p>Tissue engineering is a promising approach for generating or repairing living tissues. The development of innovative biomaterials for tissue engineering has the potential to address the unmet clinical needs in certain applications. However, before these biomaterials can be used in clinical settings, they must undergo preclinical testing to ensure safety and performance. The chicken chorioallantoic membrane (CAM) assay is a preferred screening tool for studying biocompatibility, angiogenesis, and inflammation induced by biomaterials owing to ethical and economic considerations. This CAM-based platform increased the throughput of biomaterial testing for tissue engineering before in vivo testing. In this paper, we discuss the advantages of the CAM model. We also provided a step-by-step guide for implementing the CAM model in a research laboratory, along with tips and tricks for successfully running CAM assays. Finally, we present examples of biomaterials screened using CAM assays. CAM assay is a powerful in vivo model for assessing the angiogenic potential of tissue-engineered scaffolds. This guide provides a framework for conducting the assay, but specific experimental conditions should be optimized based on the scaffold material and the research question.</p>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"112 11","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.b.35496","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142545703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Accelerated In Vitro Oxidative Degradation Testing of Ultra-High Molecular Weight Polyethylene (UHMWPE)","authors":"Tanmay Jain,&nbsp;Hunter Danesi,&nbsp;Anne Lucas,&nbsp;Benita Dair,&nbsp;Katherine Vorvolakos","doi":"10.1002/jbm.b.35495","DOIUrl":"10.1002/jbm.b.35495","url":null,"abstract":"<div>\u0000 \u0000 <p>Nonabsorbable polymers used in biomedical applications are assumed to be permanently stable based on short-term testing, but some may be susceptible to oxidative degradation over several years of implantation. Traditional in vitro oxidative degradation screenings employ hydrogen peroxide (H₂O₂) solutions. However, the inherent instability of H₂O₂ can compromise the consistency of oxidative conditions, especially over extended periods and at elevated temperatures used for accelerated testing. In this study, an automated reactive accelerated aging (aRAA) system, which integrates an electrochemical detection method and a feedback loop, was utilized to ensure precise control of H₂O₂ concentrations during polymer oxidative degradation testing. The reproducibility of the aRAA system was evaluated by comparing four identical setups. Its efficacy as an oxidation challenge was demonstrated on (i) medical-grade vitamin E (VE) blended ultra-high molecular weight polyethylene (UHMWPE) and (ii) highly crosslinked (HXL) UHMWPE as model materials. The aRAA-aged VE-UHMWPE and HXL-UHMWPE samples were also compared against samples aged via an existing accelerated aging standard, ASTM F2003-02(2022). Samples were analyzed using attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy to calculate their oxidation index per ASTM F2102-17. We observed that the aRAA system was more effective in oxidizing VE-UHMWPE and HXL-UHMWPE than the traditional ASTM F2003-02(2022) method. By providing a standardized and reliable approach to assess polymer oxidative degradation, the aRAA system could enhance the accuracy of long-term stability predictions for nonresorbable polymers in medical devices.</p>\u0000 </div>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"112 11","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142466413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biocompatible and Safe Decellularized Spinach With Antibacterial and Wound Healing Activity","authors":"Rihab Ksouri,&nbsp;Hamide Aksel,&nbsp;Hamza Saghrouchni,&nbsp;Yasemin Saygideger","doi":"10.1002/jbm.b.35489","DOIUrl":"10.1002/jbm.b.35489","url":null,"abstract":"<p>Creating acellular vascularized constructs from animal and plant tissue is one of the well-known strategies for scaffold assembly. Decellularization takes an important position among these strategies. The most common method is chemical decellularization. This approach employs high concentrations of detergents, primarily Triton X-100, sodium dodecyl sulfate (SDS), and sodium hypochlorite (SH). In this work, novel techniques for decellularizing spinach were developed using detergents frequently utilized in laboratories. Spinach leaves were decellularized using Tween-20, SDS, and SH at low concentrations to generate an acellular plant matrix for tissue engineering. We measured the quantities of DNA and protein, as well as the decellularization using hematoxylin and eosin (H&amp;E) staining. The biocompatibility and capacity of the biostructures to stimulate fibroblast wound healing were assessed using MTT and the Scratch assay. The antibacterial activity of the scaffolds was also tested against a gram-positive bacterium, <i>Staphilococcus aureus</i>, which is a common pathogen associated with wound healing. The best shape, evident vascularization, and good biocompatibility were seen in the Tween-20 decellularized samples at 1% concentration at 21°C and 37°C through the enhancement of cell proliferation and wound healing. In terms of antibacterial activity, all scaffold samples had a significant effect on <i>Staphilococcus aureus</i>, where the number of bacterial colonies in all six scaffold groups became zero after 4 h of treatment. The scaffolds also showed a 100% kill rate on <i>Staphilococcus aureus</i>, which could avoid wound infection during the repair process, and that can be suggested as a scaffold for tissue engineering applications and an important constituent for pharmacological activities.</p>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"112 10","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.b.35489","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142390836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In Vitro and In Vivo Biocompatibility of Bacterial Cellulose","authors":"Vincent-Daniel Girard,&nbsp;Jérémie Chaussé,&nbsp;Martin Borduas,&nbsp;Émile Dubuc,&nbsp;Christian Iorio-Morin,&nbsp;Simon Brisebois,&nbsp;Patrick Vermette","doi":"10.1002/jbm.b.35488","DOIUrl":"10.1002/jbm.b.35488","url":null,"abstract":"<p>Bacterial cellulose is a unique biomaterial produced by various species of bacteria that offers a range of potential applications in the biomedical field. To provide a cost-effective alternative to soft-tissue implants used in cavity infills, remodeling, and subdermal wound healing, in vitro cytotoxicity and in vivo biocompatibility of native bacterial cellulose were investigated. Cytotoxicity was assessed using a metabolic assay on Swiss 3T3 fibroblasts and INS-1832/13 rat insulinoma. Results showed no cytotoxicity, whether the cells were seeded over or under the bacterial cellulose scaffolds. Biocompatibility was performed on Sprague–Dawley rats (males and females, 8 weeks old) by implanting bacterial cellulose membranes subcutaneously for 1 or 12 weeks. The explanted scaffolds were then sliced and stained with hematoxylin and eosin for histological characterization. The first series of results revealed acute and chronic inflammation persisting over 12 weeks. Examination of the explants indicated a high number of granulocytes within the periphery of the bacterial cellulose, suggesting the presence of endotoxins within the membrane, confirmed by a Limulus amebocyte lysate test. This discovery motivated the development of non-pyrogenic bacterial cellulose scaffolds. Following this, a second series of animal experiments was done, in which materials were implanted for 1 or 2 weeks. The results revealed mild inflammation 1 week after implantation, which then diminished to minimal inflammation after 2 weeks. Altogether, this study highlights that unmodified, purified native bacterial cellulose membranes may be used as a cost-effective biomedical device provided that proper endotoxin clearance is achieved.</p>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"112 10","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.b.35488","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142365374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Biomarkers for In Vitro Thrombogenicity Assessment of Medical Device Materials","authors":"Mehulkumar Patel,&nbsp;Anna Parrish,&nbsp;Carlos Serna III,&nbsp;Megan Jamiolkowski,&nbsp;Keerthana Srinivasan,&nbsp;Richard Malinauskas,&nbsp;Qijin Lu","doi":"10.1002/jbm.b.35491","DOIUrl":"10.1002/jbm.b.35491","url":null,"abstract":"<div>\u0000 \u0000 <p>To develop standardized in vitro thrombogenicity test methods for evaluating medical device materials, three platelet activation biomarkers, beta-thromboglobulin (β-TG), platelet factor 4 (PF4), soluble p-selectin (CD62P), and a plasma coagulation marker, thrombin–antithrombin complex (TAT), were investigated. Whole blood, drawn from six healthy human volunteers into Anticoagulant Citrate Dextrose Solution A was recalcified and heparinized over a concentration range of 0.5–1.5 U/mL. The blood was incubated with test materials with different thrombogenic potentials for 60 min at 37°C, using a 6 cm²/mL material surface area to blood volume ratio. After incubation, the blood platelet count was measured before centrifuging the blood to prepare platelet-poor plasma (PPP) and platelet-free plasma (PFP) for enzyme-linked immunosorbent assay analysis of the biomarkers. The results show that all four markers effectively differentiated the materials with different thrombogenic potentials at heparin concentrations from 1.0 to 1.5 U/mL. When a donor-specific heparin concentration (determined by activated clotting time) was used, the markers were able to differentiate materials consistently for blood from all the donors. Additionally, using PFP instead of PPP further improved the test method's ability to differentiate the thrombogenic materials from the negative control for β-TG and TAT. Moreover, the platelet activation markers were able to detect reversible platelet activation induced by adenosine diphosphate (ADP). In summary, all three platelet activation markers (β-TG, PF4, and CD62P) can distinguish thrombogenic potentials of different materials and detect ADP-induced reversible platelet activation. Test consistency and sensitivity can be enhanced by using a donor-specific heparin concentration and PFP. The same test conditions are applicable to the measurement of coagulation marker TAT.</p>\u0000 </div>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"112 10","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142347406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Production and Characterization of Electrospun Chitosan, Nanochitosan and Hyaluronic Acid Membranes for Skin Wound Healing","authors":"Edimar Dal Ponte,&nbsp;Alexia de Almeida Ignatowicz,&nbsp;Gabriel Raio Volpato,&nbsp;João Vitor Taffarel,&nbsp;Priscila Ayumi Takahashi,&nbsp;Rafael Messias Luiz,&nbsp;Felipe Eduardo Bueno Silva,&nbsp;Gabriel Nardi Fraga,&nbsp;Douglas Cardoso Dragunski,&nbsp;Ana Carla Zarpelon-Schutz,&nbsp;Helton José Alves,&nbsp;Juliana Bernardi-Wenzel","doi":"10.1002/jbm.b.35485","DOIUrl":"https://doi.org/10.1002/jbm.b.35485","url":null,"abstract":"<div>\u0000 \u0000 <p>The development of new wound dressings made from biomaterials, which offer a better cost–benefit ratio and accelerate the healing process, is increasing nowadays. Various biopolymers can be electrospun to form functional membranes for wound healing. Therefore, in this study, chitosan and nanochitosan membranes with or without hyaluronic acid were prepared using the electrospinning technique, characterized and evaluated in the healing of skin wounds in rats. Chitosan and nanochitosan solutions, with or without hyaluronic acid, were prepared at concentrations of 1%–4% using PEO (polyethylene oxide) and subjected to the electrospinning process to obtain membranes characterized by scanning electron microscopy (SEM), mechanical tests, and antimicrobial activity. The healing effect of the membranes was evaluated by monitoring the area of the lesions, contraction of the wounds, histologic analysis, and induction of pro-inflammatory cytokine (IL-1 α and TNF-α) production in rats. The nanochitosan and nanochitosan membranes with hyaluronic acid achieved greater fiber diameter and uniformity, resistance, elasticity, and thermal stability, in addition to good adhesion to the wound bed and permeation capacity. Despite not presenting antimicrobial activity in vitro, they contributed to the production of pro-inflammatory interleukins in the animals tested, provided physical protection, reduced the wound area more markedly until the seventh day of the evaluation, with an acceleration of the healing process and especially when functionalized with hyaluronic acid. These results indicate that the membranes may be promising for accelerating the healing process of chronic wounds in humans.</p>\u0000 </div>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"112 10","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142324708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cationic Polystyrene Latex Nanocarriers for Immunostimulatory Long Double-Stranded RNA Delivery to Ovarian Cancer Cells","authors":"N. L. Aldor,&nbsp;N. A. Jadaa,&nbsp;S. Y. Miller,&nbsp;I. Alla,&nbsp;S. Richardson,&nbsp;V. Kitaev,&nbsp;S. J. Poynter","doi":"10.1002/jbm.b.35487","DOIUrl":"https://doi.org/10.1002/jbm.b.35487","url":null,"abstract":"<p>Long double-stranded (ds)RNA, a potent stimulator of type I interferon and the innate immune response. In the present study, we demonstrated, for the first time, the efficacy of cationic polystyrene latex nanostructures (clNPs) as a dsRNA carrier, improving cellular delivery and robustly potentiating the immunostimulatory capacity of dsRNA in the ovarian cancer cell line SKOV3. The clNPs complexed with an in vitro transcribed dsRNA molecule, were bound by SKOV3 cells, and had increased cellular association compared to uncomplexed clNPs. clNPs complexed with dsRNA induced a more robust innate immune response compared to dsRNA alone. Transcript expression of two interferon-stimulated genes, were increased 47- and 108-fold over dsRNA and induced a significant antiviral state against vesicular-stomatitis virus, resulting in a 3.3-fold improvement on the efficacy of dsRNA. These data highlight the potential of polystyrene latex nanostructures as dsRNA carriers for anticancer immunotherapies, improving the uptake and efficacy of the nucleic acid.</p>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"112 10","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.b.35487","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142316875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Osseointegration and Histopathological Evaluation of Titanium–Titanium Diboride Composite Compared to Pure Titanium Implant Materials Prepared by Powder Metallurgy (In Vivo Study)","authors":"Ali Mohammad Ali Aljafery,&nbsp;Abdalbseet A. Fatalla,&nbsp;Julfikar Haider","doi":"10.1002/jbm.b.35490","DOIUrl":"10.1002/jbm.b.35490","url":null,"abstract":"<div>\u0000 \u0000 <p>The efficacy and osseointegration rate of an implant depend on its biocompatibility. Modern implantology seeks fast and reliable osseointegration, which is essential for clinical success. The objective of this research was to assess the osseointegration and biocompatibility of a titanium–titanium diboride composite (Ti-TiB₂) in rabbits in contrast to those of pure titanium (Ti). A total of 64 cylindrical implant specimens were fabricated, consisting of two sets: pure Ti (32 implants) and Ti-TiB₂ composite (32 implants). In this study, two implants were implanted per tibia (left and right tibias) in 16 white male New Zealand rabbits, for a total of four implants per rabbit (4 × 16 = 64 implants). A pushout test was used to assess implant specimen-bone bonding after 2 and 6 weeks of healing. The experiment utilized five rabbits per healing phase, which means that 20 implants per time point were used for the pushout tests. (10 for pure Ti and 10 for the composite). Histology was used to examine the tissue response to biocompatibility, and histomorphometry was used to measure new bone growth at the two time points. With three rabbits per time point, 12 implants were employed for the histological analyses. After implantation, the pushout shear strength results revealed that the mean shear strength of the Ti-TiB₂ implant specimens (5.4 ± 0.029 MPa for 2 weeks, 7.9 ± 0.029 MPa for 6 weeks) was statistically greater (<i>p</i> &lt; 0.0001) than that of the pure Ti implant specimens (5.1 ± 0.015 MPa for 2 weeks, 6.6 ± 0.047 MPa for 6 weeks). After 2 weeks, woven bone tissues were observed around the pure titanium implants, and active osteoid tissue around the composite implants exhibited significant differences in new bone formation areas (NBFAs) (0.54 ± 0.004 mm² for Ti and 0.65 ± 0.003 mm² for the composite). After 6 weeks, there was new bone formation with osteocytes around the pure titanium implants (NBFA of 2.44 mm²) and osteoid maturation with the observation of reversal lines around the composite implants (NBFA of 2.89 mm²). The developed Ti-TiB₂ material was biocompatible and demonstrated superior bone growth compared to that of the pure Ti materials after 2 and 6 weeks.</p>\u0000 </div>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"112 10","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142307786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}