Journal of Assisted Reproduction and Genetics最新文献

{"title":"Ambient temperature transport of human oocytes: an unexpected research resource.","authors":"Maria G Gervasi, Maureen Kearnan, Ann A Kiessling, David F Albertini","doi":"10.1007/s10815-025-03548-9","DOIUrl":"10.1007/s10815-025-03548-9","url":null,"abstract":"<p><strong>Purpose: </strong>This study aimed to determine the viability and meiotic competence of human oocytes deemed not suitable for clinical use following controlled ovarian stimulation of young egg donors receiving treatment at an egg bank.</p><p><strong>Methods: </strong>A total of 432 oocytes were shipped at ambient temperature overnight, in a medium containing caffeine and dibutyryl cyclic-AMP to limit meiotic cell cycle progression, and estrogen and progesterone to mimic the intrafollicular environment. In some experiments, transport medium was also supplemented with 1 µg/ml ZnSO₄. Oocytes were either fixed immediately upon arrival or cultured for 20-24 or up to 143 h followed by fixation. Time-lapse imaging and fluorescence imaging were used to establish viability, meiotic status, and spontaneous activation.</p><p><strong>Results: </strong>Greater than 95% of transported oocytes retained viability, whether transported with or without added ZnSO₄, exhibiting meiotic progression and/or spontaneous activation following overnight culture. Time-lapse imaging and fluorescence imaging revealed a higher incidence of spontaneous activation and subsequent cleavage activity for up to 5 days in culture in samples transported in ZnSO₄.</p><p><strong>Conclusions: </strong>Under the experimental conditions described here, immature human oocytes retain viability and meiotic competence following ambient temperature transport, providing a novel and experimentally tractable resource for future research in human oocyte biology and the development of human parthenote stem cells.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144340170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing the risk of ectopic pregnancy following euploid frozen embryo transfer: a retrospective cohort study.","authors":"A Salame, B Lawrenz, E Kalafat, R Del Gallego, I ElKhatib, A Bayram, H Fatemi","doi":"10.1007/s10815-025-03560-z","DOIUrl":"10.1007/s10815-025-03560-z","url":null,"abstract":"<p><strong>Purpose: </strong>To evaluate possible risk factors for an ectopic pregnancy after euploid frozen embryo transfer (FET) at blastocyst stage.</p><p><strong>Methods: </strong>Retrospective cohort study of women with a positive pregnancy test after euploid frozen embryo transfer at blastocyst stage between April 2017 and June 2023.</p><p><strong>Results: </strong>An ectopic pregnancy occurred in 21 patients (1.19%) out of 1758 patients. Patient characteristics age, BMI, smoking status, infertility type, cycle/embryo characteristics (type of endometrial preparation and embryo quality), and presence of an isthmocele (yes/no) were not significantly different between the patients with versus without an ectopic pregnancy. The ratio measured to expected hCG levels was significantly lower in patients with ectopic pregnancy when compared to those with clinical pregnancy (0.7 vs 1, P < 0.001). The multinomial regression analysis showed increased odds of an ectopic pregnancy (EP) compared to an ongoing clinical pregnancy with lower observed/expected hCG levels in both single and double embryo transfers (OR, 0.4; 95% CI, 0.07-0.25; P < 0.001 and OR, 0.16; 95% CI, 0.09-0.30; P < 0.001).</p><p><strong>Conclusion: </strong>The risk of ectopic pregnancy in euploid frozen embryo transfer cycles at the blastocyst stage is comparable to spontaneous conceptions. Despite the huge sample size of euploid FET cycles, due to the very low number of ectopic pregnancies, we were unable to identify a particular risk factor for ectopic implantation.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144336536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: A novel small droplets freezing method for human sperm cryopreservation: maximizing utilization of limited samples.","authors":"Yael Harir, Rona Halevy Amiran, Miriam Almagor, Sheila Fieldust, Zeev Shoham, Yuval Or","doi":"10.1007/s10815-025-03561-y","DOIUrl":"10.1007/s10815-025-03561-y","url":null,"abstract":"","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144333228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of breastfeeding on treatment outcomes in in-vitro fertilization frozen embryo transfer cycles.","authors":"Alyssa Hochberg, Nir Kugelman, Uri Amikam, Victoria Bleau, Tzippy Shochat, Eva Suarthana, William Buckett","doi":"10.1007/s10815-025-03556-9","DOIUrl":"10.1007/s10815-025-03556-9","url":null,"abstract":"<p><strong>Purpose: </strong>While breastfeeding is known to impact unassisted conception, its effect on fertility treatment outcomes remains unclear. This study aimed to evaluate the effect of breastfeeding on in-vitro fertilization (IVF) frozen embryo transfer (FET) outcomes.</p><p><strong>Methods: </strong>A retrospective cohort study at a single, urban, Canadian Fertility center, including women who underwent an FET(2015-2023). Breastfeeding women (study group) were compared to non-breastfeeding controls, matched 1:1 for age, treatment protocol, and year. We compared outcomes between the two groups. Primary outcomes were pregnancy rate (PR), clinical pregnancy rate (CPR),and live birth rate (LBR). Secondary outcomes included peak endometrial thickness prior to progesterone supplementation, biochemical PR and miscarriage rate. Multivariate logistic regression controlled for confounders. Two sub-analyses within the breastfeeding group examined treatment outcomes by breastfeeding duration (≤ 12 months; > 12 months) and prolactin levels (normal;elevated).</p><p><strong>Results: </strong>Overall, 118 women met inclusion criteria (59 breastfeeding, 59 controls). Breastfeeding women, compared to controls,were characterized by a younger median age at initial oocyte collection (34.8 years(31.9-37.11) vs. 35.85 years(33.2-39.2),p = 0.044); higher median parity (1(1-1) vs. 0(0-1), p < 0.0001);and higher rates of irregular cycles (35.59% vs. 18.64%,p = 0.016);and elevated prolactin levels (16.95% vs. 1.69%,p = 0.003). Utilizing multivariate logistic regression adjusting for breastfeeding status,parity, age at initial oocyte collection, and cycle regularity, only maternal age at oocyte collection was associated with PR (adjusted odds ratio 0.84, 95% confidence interval 0.75-0.94,p = 0.003). Both sub-analyses revealed similar PRs, CPRs and LBRs between groups.</p><p><strong>Conclusions: </strong>Breastfeeding during an FET cycle was not associated with treatment outcomes, including PR,CPR, or LBR, providing cautious reassurance to physicians and patients.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144333229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparing programmed, modified natural and natural cycle frozen embryo transfer on obstetric outcomes in polycystic ovary syndrome: a national cohort study.","authors":"Eun Hee Yu, Hyun Joo Lee, Jong Kil Joo, Yong Jin Na","doi":"10.1007/s10815-025-03523-4","DOIUrl":"https://doi.org/10.1007/s10815-025-03523-4","url":null,"abstract":"<p><strong>Purpose: </strong>Polycystic ovary syndrome (PCOS) is a common endocrine disorder associated with adverse reproductive and obstetric outcomes, particularly in pregnancies achieved through assisted reproductive technologies. This study aimed to compare obstetric outcomes among programmed cycle-frozen embryo transfer (PC-FET), modified natural cycle-frozen embryo transfer (mNC-FET), and natural cycle-frozen embryo transfer (NC-FET) in women with PCOS using a nationwide Korean health claims database.</p><p><strong>Methods: </strong>This population-based cohort study utilized data from the National Health Insurance Service-National Health Information Database of South Korea. A retrospective analysis was conducted on 3,873 PCOS patients with singleton pregnancies following FET between October 2017 and December 2021. Patients were categorized into PC-FET, mNC-FET, and NC-FET groups based on prescription records. Obstetric outcomes were analyzed using logistic regression models adjusted for age and medical comorbidities.</p><p><strong>Results: </strong>mN-FET was associated with significantly lower rates of miscarriage (23.9%) compared to PC-FET (31.3%) and NC-FET (32.0%), as well as a higher term birth rate (69.6% vs. 61.7% and 60.2%, respectively). mNC-FET also showed significantly lower rates of hypertensive disorders of pregnancy, gestational hypertension, cesarean section, and emergency cesarean section compared to PC-FET. No significant differences were observed between mNC-FET and NC-FET in most obstetric complications.</p><p><strong>Conclusion: </strong>mNC-FET was associated with favorable obstetric outcomes compared to PC-FET and NC-FET in women with PCOS. These findings suggest that mNC-FET may be an effective and safer endometrial preparation strategy in this population. Further prospective studies are needed to validate these results and optimize FET protocols based on individual patient characteristics.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144325890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mental health screening and psychological support should be the standard of care in fertility clinics.","authors":"Alison J Meyers","doi":"10.1007/s10815-025-03543-0","DOIUrl":"https://doi.org/10.1007/s10815-025-03543-0","url":null,"abstract":"<p><p>Infertility affects up to one in six individuals, a common diagnosis that, like most chronic health conditions, comes with an array of physical, emotional, social, and economical challenges. Currently, less than a fourth of individuals undergoing care for infertility utilize mental health services for a myriad of reasons including but not limited to access, cost, travel time, and lack of knowledge of what care would entail. By improving psychological well-being, infertility patients may increase their relationship satisfaction, coping mechanisms for grief and guilt that frequently come with the diagnosis, adherence to treatment, and even chances at achieving a successful pregnancy. In this paper, I argue that mental health services should be incorporated into standard fertility care visits, spearheaded by licensed and experienced mental health professionals to screen and assess a patient's needs and risk of distress, to not only achieve the aforementioned benefits of mental health counseling, but also improve their overall well-being.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144317027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-Müllerian hormone binds heat shock protein glucose-regulated protein 78 in human follicular fluid.","authors":"Caitlin R Sacha, Nicholas Nagykery, Marie-Charlotte Meinsohn, Karine de Mattos, James A Howard, Hadi Ramadan, Evelyn Minis, LiHua Zhang, Victoria Fitz, Karissa Hammer, Irene C Souter, Thomas B Thompson, Patricia K Donahoe, David Pépin","doi":"10.1007/s10815-025-03541-2","DOIUrl":"https://doi.org/10.1007/s10815-025-03541-2","url":null,"abstract":"<p><strong>Purpose: </strong>Anti-Müllerian hormone (AMH) regulates key steps in folliculogenesis, yet unlike other TGF-β family members, no soluble AMH-binding proteins have been identified. We hypothesized that AMH may bind shuttling proteins in human follicular fluid (FF).</p><p><strong>Methods: </strong>Discarded pooled FF was collected from women undergoing oocyte retrieval at an academic fertility center. AMH-binding soluble proteins in FF were co-immunoprecipitated with AMH, identified by mass spectrometry, and their association was validated by western blot. We characterized the spatiotemporal expression of candidate binding protein glucose-regulated protein 78 (GRP78) in the mouse ovary across the cycle by immunohistochemistry. We used enzyme-linked immunoassay (ELISA) to measure AMH and GRP78 concentrations in patient FF. We used transient transfections in Chinese hamster ovary (CHO) cells to understand the interaction between AMH and GRP78.</p><p><strong>Results: </strong>Co-immunoprecipitation of AMH in FF identified multiple soluble AMH-binding candidates in the heat-shock protein family, which was confirmed by reciprocal co-immunoprecipitation. We found that GRP78, the most abundant candidate, was enriched in antral follicles of mice at estrus and was present in higher concentrations in human FF than AMH. Finally, we found that AMH overexpression increased endoplasmic reticulum stress and induced GRP78 expression in CHO cells; further ectopic overexpression of GRP78 facilitated the secretion of AMH.</p><p><strong>Conclusion: </strong>This work describes AMH-binding protein candidates identified in human FF and suggests that GRP78 may chaperone AMH during secretion and remain bound in FF. Further study is warranted to understand how heat shock proteins may modulate other biological aspects of AMH and their effects on fertility.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144309986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Embryoid bodies as a model system for exploring early human embryonic development.","authors":"Daria I Zhigalina, Evgeny V Denisov, Igor N Lebedev, Nikolay A Skryabin","doi":"10.1007/s10815-025-03546-x","DOIUrl":"https://doi.org/10.1007/s10815-025-03546-x","url":null,"abstract":"<p><p>This review article aims to summarize the existing concepts related to embryoid bodies (EBs) and explore their potential as a model system for studying various aspects of human embryonic development. The review involves the collection and analysis of information about the characteristics of EBs and the properties of stem cells that give rise to them. The results show that EBs derived from pluripotent stem cells are a promising model that closely replicates processes occurring in human embryos after implantation. The review also provides a comparative analysis of the advantages and limitations of models based on induced pluripotent stem cells (iPSCs) and embryonic stem cells (ESCs), with particular attention given to summarizing the results of limited studies on RNA sequencing in individual cells from human and mouse EBs. In conclusion, we would like to emphasize that embryoid bodies are an effective model system for studying early human embryogenesis. This opens up new possibilities for reproductive genetics and medicine.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144317026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between ovarian hyperstimulation syndrome and adverse obstetric and neonatal outcomes in Korea: a nationwide health insurance database study.","authors":"Yunjeong Park, Heechul Jeong, Jung-Ho Shin, Geum Joon Cho, Yong Jin Kim","doi":"10.1007/s10815-025-03542-1","DOIUrl":"https://doi.org/10.1007/s10815-025-03542-1","url":null,"abstract":"<p><strong>Purpose: </strong>To investigate the association of ovarian hyperstimulation syndrome (OHSS) and adverse maternal and neonatal outcomes.</p><p><strong>Methods: </strong>This retrospective study used combined Korea National Health Insurance (KNHI) claims and National Health Screening Program for Infants and Children data. Women who gave birth through in vitro fertilization using fresh embryo transfer during 2017-2022 were enrolled. Z-scores were calculated. Student's t-test, analysis of variance, Pearson's chi-squared (χ²) test, and multivariate logistic regression analyses were performed.</p><p><strong>Results: </strong>The non-OHSS group included 27,337 women, while the OHSS group included 1,570 women (mild to moderate, 1,308; severe, 262). The OHSS group was significantly younger (34.86 ± 3.54 vs. 36.21 ± 3.58 years, p < 0.0001) and had significantly higher rates of primiparity, cesarean section, and polycystic ovary syndrome (88.98% vs. 82.89%, p < 0.0001; 75.8% vs. 65.75%, p < 0.0001; and 29.24% vs. 14.37%, p < 0.0001, respectively). OHSS was associated with a decreased incidence of low birth weight and with an increased incidence of macrosomia (adjusted odds ratio [95% confidence interval], 0.53 [0.38-0.72], p < 0.0001; and 1.83 [1.42-2.35], p < 0.0001). OHSS was associated with an increased incidence of transient tachypnea of the newborn (TTN) and respiratory distress syndrome (RDS) (1.32 [1.02-1.71], p = 0.03; and 1.57 [1.30-1.91], p < 0.0001).</p><p><strong>Conclusion: </strong>OHSS is associated with a decreased incidence of low birth weight and an increased incidence of macrosomia, TTN, and RDS.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144317025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cumulative live birth rates among over 13,000 poor ovarian responders from 2015 to 2023: a retrospective cohort study assessing the efficacy of natural cycle and controlled ovarian stimulation.","authors":"Jiaxin Lv, Wei Guo, Tian Tian, Lixue Chen, Xiumei Zhen, Rong Li, Jie Qiao, Rui Yang","doi":"10.1007/s10815-025-03544-z","DOIUrl":"https://doi.org/10.1007/s10815-025-03544-z","url":null,"abstract":"<p><strong>Background: </strong>Natural cycle in vitro fertilization (NC-IVF) represents a convenient and safe assisted reproductive technology, making it particularly advantageous for patients with poor ovarian response (POR). This research evaluates the effectiveness of NC-IVF for women with POR, aiming to inform personalized treatment decisions.</p><p><strong>Method: </strong>This retrospective cohort study encompassed 13,013 cycles involving women diagnosed with poor ovarian response according to the Bologna criteria. These patients underwent either natural cycles or controlled ovarian stimulation cycles. The primary outcome measure was the cumulative live birth rates, and the secondary outcomes included laboratory and clinical outcomes.</p><p><strong>Results: </strong>A total of 1073 natural cycles and 11,940 COS cycles were analyzed, with 5956 undergoing low-dose gonadotropin treatment and 5984 receiving high-dose gonadotropin. The basic characteristics were comparable among the three groups. In both fresh and frozen embryo transfer cycles, clinical pregnancy rates, implantation rates, and live birth rates were comparable across all three groups. Furthermore, no statistically significant differences were observed in cumulative live birth rates or time to first live birth between the groups examined. Expenditures in the natural cycle group were substantially lower than those in both COS cohorts. Importantly, further analysis indicated that there were no significant differences among the three groups concerning either pregnancy complications or neonatal outcomes.</p><p><strong>Conclusion: </strong>Our findings indicate that for women demonstrating a poor ovarian response, NC treatment yields comparable pregnancy and live birth rates when compared to controlled ovarian stimulation methods. The natural cycle represents a safe, effective, and economically viable treatment option for this patient population.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144293775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}