Journal of Assisted Reproduction and Genetics最新文献_第2页

Serum progesterone variability on embryo transfer day in hormone replacement therapy cycles using intramuscular injections during frozen embryo transfers. 冷冻胚胎移植期间肌内注射激素替代治疗周期中胚胎移植日血清孕酮变化。

IF 2.7 3区医学

Journal of Assisted Reproduction and Genetics Pub Date : 2025-09-09 DOI: 10.1007/s10815-025-03646-8

Fazilet Kübra Boynukalın, Yusuf Aytaç Tohma, Berfu Demir, Meral Gültomruk, Nikolaos P Polyzos, Mustafa Bahçeci, Gürkan Bozdağ

{"title":"Serum progesterone variability on embryo transfer day in hormone replacement therapy cycles using intramuscular injections during frozen embryo transfers.","authors":"Fazilet Kübra Boynukalın, Yusuf Aytaç Tohma, Berfu Demir, Meral Gültomruk, Nikolaos P Polyzos, Mustafa Bahçeci, Gürkan Bozdağ","doi":"10.1007/s10815-025-03646-8","DOIUrl":"https://doi.org/10.1007/s10815-025-03646-8","url":null,"abstract":"Purpose: To assess the intra-individual variability of serum progesterone (P) levels on embryo transfer (ET) day, when the same dose of intramuscular progesterone (IM-P) was used in two consecutive hormone replacement therapy (HRT) frozen embryo transfer (FET) cycles.Methods: A total of 75 patients undergoing two consecutive HRT-FET cycles in one year performed at Bahceci Ankara IVF Center between November 2019 and February 2022 were retrospectively analyzed. Serum P levels were measured at the 117th-119th hours of support by a single laboratory. The two measurements of P levels performed on the day of the first and the second FET were compared to evaluate the intra-individual variability of serum P levels.Results: Comparisons between the 1st and 2nd FET cycles revealed statistically significant intra-individual variation, with an average difference of -2.47 ng/mL (95% CI: -4.65 to -0.29, p = 0.027) between the two consecutive measurements. To assess their consistency, the limit of agreement was also tested with the Bland-Altman method, in which the mean difference (+ 1.96 × SD and -1.96 × SD) was -2.47 (16.1 and -21.1). Based on a previous study, the frequency of low P levels, as expressed by being > 20.6 ng/mL on ET day, was similar between the 1st and 2nd FET cycles (14.7% vs. 9.3%, p = 0.31). Notably, most patients had improved P levels in the second cycle if initially low, while decreases were rare among those with initially higher levels.Conclusion: Serum P levels may vary within the same individual across FET cycles despite the use of the same dosage of IM-P. Increasing maternal age, high body mass index, and fluctuating estradiol levels on the day of ET were identified as risk factors contributing to this variability.","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145029918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Determinants of fetal growth variability in ART conceptions. ART受孕中胎儿生长变异的决定因素。

IF 2.7 3区医学

Journal of Assisted Reproduction and Genetics Pub Date : 2025-09-08 DOI: 10.1007/s10815-025-03655-7

Mirko Pozzoni, Serena Girardelli, Paolo I Cavoretto

引用次数: 0

Ongoing pregnancy rate is associated with trigger-day progesterone level in letrozole/clomiphene intrauterine insemination cycles. 来曲唑/克罗米芬宫内人工授精周期中持续妊娠率与触发日孕酮水平相关。

IF 2.7 3区医学

Journal of Assisted Reproduction and Genetics Pub Date : 2025-09-07 DOI: 10.1007/s10815-025-03654-8

Carrie A Malcom, Kerry Flannagan, Phillip A Romanski, Anthony N Imudia

{"title":"Ongoing pregnancy rate is associated with trigger-day progesterone level in letrozole/clomiphene intrauterine insemination cycles.","authors":"Carrie A Malcom, Kerry Flannagan, Phillip A Romanski, Anthony N Imudia","doi":"10.1007/s10815-025-03654-8","DOIUrl":"https://doi.org/10.1007/s10815-025-03654-8","url":null,"abstract":"Purpose: Prior studies in fresh embryo transfer IVF cycles have associated elevated serum progesterone level on day of ovulatory trigger, particularly if ≥ 1.5 ng/ml, with decreased pregnancy rates. A similar association has been found in intrauterine insemination (IUI) cycles using gonadotropins for ovulation induction. The purpose of this study was to evaluate the association of trigger-day progesterone level with pregnancy rates in IUI cycles using oral ovulation induction agents.Methods: A retrospective cohort study was conducted at a multicenter private practice. 4,866 IUI cycles using letrozole or clomiphene were analyzed from January 1, 2017, to December 31, 2023. The primary outcome measure was ongoing pregnancy per cycle as a function of the serum progesterone level obtained on the day of ovulatory trigger. Secondary outcome measures were positive pregnancy, clinical pregnancy, and pregnancy loss.Results: When compared to cycles with trigger-day progesterone < 1 ng/ml, ongoing pregnancy was significantly lower when progesterone was ≥ 1.5 ng/ml (11.9% versus 5.6%; Risk Ratio (RR) 0.46 (95% CI 0.25-0.84)). Ongoing pregnancy was comparable when progesterone was < 1 ng/ml or 1-1.49 ng/ml (11.9% versus 9.2%; (RR 0.75 (95% CI 0.55-1.03)). Positive pregnancy and clinical intrauterine pregnancy outcomes were also significantly lower in the ≥ 1.5 ng/ml versus < 1 ng/ml group, but comparable in the < 1 ng/ml and 1-1.49 ng/ml groups. Pregnancy loss was not significantly different between groups.Conclusion: Pregnancy outcomes are optimized when trigger-day progesterone level is < 1.5 ng/ml in IUI cycles using oral ovulation induction agents, highlighting the value of obtaining a progesterone level on the day of ovulatory trigger.","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145015469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correlation between AMH levels and embryonic aneuploidy rate in PGT-A patients: a retrospective study. AMH水平与PGT-A患者胚胎非整倍体率的相关性：一项回顾性研究。

IF 2.7 3区医学

Journal of Assisted Reproduction and Genetics Pub Date : 2025-09-04 DOI: 10.1007/s10815-025-03619-x

Shufang Tang, Panpan Zhao, Kaixuan Sun, Qian Zhang, Yuexin Yu

{"title":"Correlation between AMH levels and embryonic aneuploidy rate in PGT-A patients: a retrospective study.","authors":"Shufang Tang, Panpan Zhao, Kaixuan Sun, Qian Zhang, Yuexin Yu","doi":"10.1007/s10815-025-03619-x","DOIUrl":"https://doi.org/10.1007/s10815-025-03619-x","url":null,"abstract":"Objective: The association between anti-Müllerian hormone (AMH) levels and embryonic aneuploidy rates was investigated by analyzing clinical and embryo laboratory data from patients with preimplantation genetic testing for aneuploidy (PGT-A). However, the nonlinear relationship and threshold effect of AMH on aneuploidy risk remain poorly understood.Methods: This retrospective study analyzed the clinical data of 819 PGT-A cycles performed between January 2018 and August 2024 at the General Hospital of Northern Theater Command. We used restricted cubic spline (RCS) to investigate the dose-response relationship between the AMH levels and aneuploidy rate, adjusting for potential confounders.Results: Significant differences were observed in normal fertilization rates, day 3 high-quality embryo rates, blastocyst formation rates, euploidy embryo rates, aneuploid embryo rates, and mosaic embryo rates among the three AMH groups (P < 0.05). A statistically significant nonlinear relationship between AMH levels and aneuploidy rate was identified (P < 0.05). RCS and threshold effect analyses revealed that the risk of a positive (≥ 50%) aneuploidy rate increased by 40% for each 1-unit decrease in AMH when AMH ≤ 2.54 ng/mL.Conclusions: In the PGT-A population, advanced maternal age (AMA), recurrent spontaneous abortion (RSA), or recurrent implantation failure (RIF) have been identified as contributing factors. After adjusting for potential confounders such as female age, AMH remains a significant risk factor for embryonic aneuploidy rates. The findings suggested that lower AMH levels are associated with a higher risk of embryonic aneuploidy, indicating that ovarian reserve function may be correlated with oocyte quality. These results provide new insights for individualized decision-making in assisted reproduction. Trial registration ChiCTR2500099710 (03/27/2025).","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144992726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Screening for non-invasive rsRNA biomarkers to assess embryo quality utilizing ultra-sensitive pandora sequencing combined with machine learning. 利用超灵敏潘多拉测序结合机器学习筛选非侵入性rsRNA生物标志物以评估胚胎质量。

IF 2.7 3区医学

Journal of Assisted Reproduction and Genetics Pub Date : 2025-09-04 DOI: 10.1007/s10815-025-03641-z

Lina Wei, Songbang Ou, Xinsheng Zhong, Zhengjie Liang, Yuhe Hong, Xuemin Yang, Yanfei Zhang, Yi Li

{"title":"Screening for non-invasive rsRNA biomarkers to assess embryo quality utilizing ultra-sensitive pandora sequencing combined with machine learning.","authors":"Lina Wei, Songbang Ou, Xinsheng Zhong, Zhengjie Liang, Yuhe Hong, Xuemin Yang, Yanfei Zhang, Yi Li","doi":"10.1007/s10815-025-03641-z","DOIUrl":"https://doi.org/10.1007/s10815-025-03641-z","url":null,"abstract":"Purpose: Accurate embryo selection is vital for the success of in vitro fertilization (IVF); however, existing morphological scoring methods are inherently subjective and fail to capture underlying molecular alterations. This study aimed to identify non-invasive molecular markers for embryo quality assessment by analyzing highly modified ribosomal small RNAs (rsRNAs) in embryo culture medium using ultra-sensitive sequencing and machine learning.Methods: Ultra-sensitive Pandora sequencing was employed to profile rsRNAs in embryo culture medium. Machine learning algorithms were used to identify rsRNA biomarkers linked to embryo quality. Candidate rsRNAs were further validated via quantitative reverse transcription polymerase chain reaction (qRT-PCR).Results: Four candidate rsRNAs (5S, 5.8S, 28-1S, 28-2S) were significantly associated with embryo quality through machine learning analysis, achieving high predictive accuracy (AUC = 0.955) in cross-validation. qRT-PCR confirmed that 5.8S and 28-2S rsRNA levels were markedly elevated in the culture medium of high-quality embryos.Conclusion: Specific rsRNAs, particularly 5.8S and 28-2S, may serve as non-invasive biomarkers for embryo selection in IVF. These findings highlight the potential roles of rsRNAs in embryonic development and provide a molecular basis for improving IVF outcomes.","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145000666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Vaginal microbiota correlations to gynecological symptoms, intimate hygiene practices, and background parameters of IVF patients: a cross-sectional study. 阴道微生物群与IVF患者妇科症状、亲密卫生习惯和背景参数的相关性：一项横断面研究

IF 2.7 3区医学

Journal of Assisted Reproduction and Genetics Pub Date : 2025-09-01 DOI: 10.1007/s10815-025-03629-9

Thor Haahr, Sophie Ovesen, Nina la Cour Freiesleben, Mette Brix Jensen, Helle Olesen Elbaek, Birgit Alsbjerg, Rita Laursen, Malene Tanderup, Lisbeth Prætorius, Henriette Svarre Nielsen, Anja Pinborg, Vibeke Hartvig, Thomas Roland Pedersen, Jørgen Skov Jensen, Peter Humaidan

{"title":"Vaginal microbiota correlations to gynecological symptoms, intimate hygiene practices, and background parameters of IVF patients: a cross-sectional study.","authors":"Thor Haahr, Sophie Ovesen, Nina la Cour Freiesleben, Mette Brix Jensen, Helle Olesen Elbaek, Birgit Alsbjerg, Rita Laursen, Malene Tanderup, Lisbeth Prætorius, Henriette Svarre Nielsen, Anja Pinborg, Vibeke Hartvig, Thomas Roland Pedersen, Jørgen Skov Jensen, Peter Humaidan","doi":"10.1007/s10815-025-03629-9","DOIUrl":"https://doi.org/10.1007/s10815-025-03629-9","url":null,"abstract":"Purpose: To investigate baseline parameters and vaginal intimate hygiene habits comparing IVF patients with and without vaginal dysbiosis, a cross-sectional study.Methods: Patients were grouped by the presence of vaginal dysbiosis status determined by a qPCR method (high quantities of Gardnerella spp. and/or Fannyhessea vaginae) and a 16S rRNA gene sequencing-based method (VALENCIA). Patients were asked to fill out a questionnaire alongside an interview with healthcare personnel. Prevalence ratios (PR) were computed in case of statistically significant findings between groups.Results: Among 1511 patients, the prevalence of vaginal dysbiosis by qPCR was 34%, and the prevalence of community state type IV was 32%. Prevalence of vaginal dysbiosis was higher with increasing BMI, BMI 25-30 (PR 1.23 (95% CI 1.05-1.44) and BMI > 30 (PR 1.50 (1.24-1.82). Fishy odor was reported relatively rarely, but vaginal dysbiosis was significantly more prevalent in the presence of fishy odor, PR 1.72 (95% CI 1.39-2.11). Finally, vaginal douching was highly prevalent and correlated significantly to vaginal dysbiosis, PR 1.31 (95% CI 1.11-1.53). In a sub-study, we interviewed N = 10 women about vaginal douching, and all of them reported the use of douching with the intention to feel clean, thus contradicting general recommendations.Conclusions: The high prevalence of vaginal dysbiosis in IVF patients is predominantly a subclinical condition which is linked to lifestyle and intimate hygiene habits.","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144955591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

LH prevents AMH action via receptor downregulation in human primary granulosa lutein cells. 在人原代颗粒叶黄素细胞中，LH通过受体下调来阻止AMH的作用。

IF 2.7 3区医学

Journal of Assisted Reproduction and Genetics Pub Date : 2025-08-28 DOI: 10.1007/s10815-025-03635-x

Francesca Liuzzi, Clara Lazzaretti, Serena De Carlini, Alice Michelini, Manuela Simoni, Livio Casarini, Antonio La Marca

{"title":"LH prevents AMH action via receptor downregulation in human primary granulosa lutein cells.","authors":"Francesca Liuzzi, Clara Lazzaretti, Serena De Carlini, Alice Michelini, Manuela Simoni, Livio Casarini, Antonio La Marca","doi":"10.1007/s10815-025-03635-x","DOIUrl":"https://doi.org/10.1007/s10815-025-03635-x","url":null,"abstract":"Purpose: Gonadotropins and anti-Müllerian hormone (AMH) regulate reproductive development and ovarian function. While AMH plays a well-established role in early folliculogenesis by counteracting gonadotropins, luteinizing hormone (LH) becomes more active later, during the antral phase, when granulosa and theca cells express their respective receptors. There are hints suggesting the existence of an interplay between these hormones regulating granulosa cell functions at late stages of the folliculogenesis., but the mechanisms remain unclear. In this context, we explored whether gonadotropin LH can modulate AMH action in cells of ovarian origin.Methods: Primary human granulosa lutein cells isolated from in vitro fertilization (IVF) patients were pretreated with recombinant LH, followed by stimulation with recombinant AMH. AMH receptor type II (AMHR2) expression, AMH signaling activation, and the expression of AMH-responsive genes were assessed through RT-PCR, ELISA, and Western blotting. The involvement of the LH receptor was confirmed using siRNA-mediated knockdown.Results: Our findings showed that LH treatment downregulates AMHR2 transcripts and protein, impairing AMH-induced phosphorylation of small mothers against decapentaplegic (SMAD) 1, 5, and preventing osterix (OSX) and matrix metalloprotease 2 (MMP2) gene expression.Conclusions: These data are consistent with the possible interplay between gonadotropins and AMH in cells from antral/luteal stages and provide the molecular basis for further studies evaluating the impact of LH in modulating AMH signaling and follicular responsiveness during the antral stage.","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144955593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances in supplementation strategies for enhancing bovine in vitro embryo production: a review of growth factors and antioxidants. 提高牛体外胚胎生产的补充策略进展：生长因子和抗氧化剂的综述。

IF 2.7 3区医学

Journal of Assisted Reproduction and Genetics Pub Date : 2025-08-27 DOI: 10.1007/s10815-025-03636-w

Onalenna Kereilwe

{"title":"Advances in supplementation strategies for enhancing bovine in vitro embryo production: a review of growth factors and antioxidants.","authors":"Onalenna Kereilwe","doi":"10.1007/s10815-025-03636-w","DOIUrl":"https://doi.org/10.1007/s10815-025-03636-w","url":null,"abstract":"The optimization of bovine embryo culture media is crucial for enhancing reproductive efficiency in livestock production. This comprehensive review evaluates the efficacy of specific growth factors and antioxidants used in bovine embryo culture, focusing on their impact on embryo development and viability. Bovine embryos are pivotal in agricultural practices, and the quality of embryo culture significantly influences pregnancy rates and overall herd productivity. This review synthesizes current research on the molecular mechanisms by which these supplements promote optimal embryo development, including pathways regulating cell proliferation, oxidative stress, and apoptosis. Practical implications for practitioners in bovine reproduction are outlined, with guidance on supplement concentrations, timing of administration, and formulation strategies to enhance embryo quality and increase pregnancy success. Furthermore, the review identifies gaps in current research and suggests future directions, such as exploring synergistic combinations of supplements, optimizing dose-response strategies, and assessing long-term effects post-transfer. In conclusion, optimizing culture media through the strategic use of growth factors and antioxidants is essential for advancing bovine reproductive technologies. This review serves as a valuable resource for researchers and practitioners aiming to improve the consistency and effectiveness of bovine in vitro embryo production, ultimately contributing to enhanced livestock productivity and sustainability.","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144955291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of bioavailable inhibin B in predicting oocyte yield in expected poor responders: the forgotten marker? 生物可利用抑制素B在预测预期不良应答者卵母细胞产量中的作用：被遗忘的标记？

IF 2.7 3区医学

Journal of Assisted Reproduction and Genetics Pub Date : 2025-08-27 DOI: 10.1007/s10815-025-03637-9

Laura Melado, Ajay Kumar, Bhanu Kalra, Erkan Kalafat, Barbara Lawrenz, Human Fatemi

{"title":"The role of bioavailable inhibin B in predicting oocyte yield in expected poor responders: the forgotten marker?","authors":"Laura Melado, Ajay Kumar, Bhanu Kalra, Erkan Kalafat, Barbara Lawrenz, Human Fatemi","doi":"10.1007/s10815-025-03637-9","DOIUrl":"https://doi.org/10.1007/s10815-025-03637-9","url":null,"abstract":"Background: Inhibin B (InhB) is secreted by granulosa cells and reflects early follicular activity. While anti-Müllerian hormone (AMH) and antral follicle count (AFC) are established ovarian reserve markers, the role of bioavailable inhibin B (bio-InhB) in predicting oocyte yield remains uncertain. This is particularly relevant in women with low ovarian reserve, where reliable prediction of ovarian response is clinically important.Objective: To assess whether baseline bio-InhB levels correlate with oocyte yield and add predictive value beyond AMH and AFC in women with low ovarian reserve undergoing IVF/ICSI.Methods: This was a prospective observational study including 72 women with AMH < 1.1 ng/mL undergoing ovarian stimulation for IVF/ICSI using a GnRH-antagonist protocol. Baseline serum bio-InhB was measured on cycle day 2/3. Ovarian response was assessed by the number of cumulus-oocyte complexes (COCs) and metaphase II (MII) oocytes retrieved.Results: Bio-InhB showed moderate correlation with AFC (rs = 0.38), AMH (rs = 0.35), and oocyte yield (COCs rs = 0.32; MII rs = 0.35). Women with high bio-InhB levels had significantly higher AFC and COC counts. However, in multivariate analysis, bio-InhB was not a significant predictor of oocyte yield, and its inclusion in predictive models did not improve accuracy based on Root Mean Square Error (RMSE) comparison (p = 0.272).Conclusion: Although bio-InhB correlates with ovarian reserve markers and oocyte yield, it does not improve predictive accuracy beyond AMH and AFC. It may still serve as a complementary marker in selected cases with discordant ovarian reserve profiles or unreliable AMH results, such as in cases of AMH mutations.Trial registration: Clinicaltrials.gov (NCT03826888).","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144955612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chronic endometritis and its association with implantation history, BCL6, and ERA in infertility patients. 不孕症患者慢性子宫内膜炎及其与着床史、BCL6和ERA的关系

IF 2.7 3区医学

Journal of Assisted Reproduction and Genetics Pub Date : 2025-08-26 DOI: 10.1007/s10815-025-03614-2

Bahar D Yilmaz, Kaia M Schwartz, Meagan Chan, Marcelle I Cedars, Hakan Cakmak, David Huang

{"title":"Chronic endometritis and its association with implantation history, BCL6, and ERA in infertility patients.","authors":"Bahar D Yilmaz, Kaia M Schwartz, Meagan Chan, Marcelle I Cedars, Hakan Cakmak, David Huang","doi":"10.1007/s10815-025-03614-2","DOIUrl":"https://doi.org/10.1007/s10815-025-03614-2","url":null,"abstract":"Purpose: To compare the prevalence of chronic endometritis (CE) in patients with and without a history of implantation failure and to assess whether CE is associated with BCL6 overexpression or a nonreceptive endometrial receptivity analysis (ERA).Methods: Retrospective cohort study of infertility patients at the University of California, San Francisco (01/2019-12/2024) who underwent evaluation for CE. Endometrial biopsies were performed in the luteal phase. CE was diagnosed by CD138 immunostaining performed by a single third-party laboratory (ReceptivaDx™). Concurrent BCL6 (ReceptivaDx™) and ERA® (Igenomix) results from the same tissue were analyzed when performed. CE prevalence was compared based on implantation history, and associations with BCL6 and ERA findings were evaluated.Results: A total of 314 patients underwent endometrial sampling for CE evaluation; 43 were excluded due to prior antibiotic exposure. Of the 271 biopsies analyzed, CE was diagnosed in 7.4% (20/271) of cases, with no significant difference between those with and without prior implantation failure (7.9% vs. 6.3%; p = 0.645). Among patients with concurrent BCL6 testing, overexpression was identified in 45.0% of the CE group and 41.9% of the non-CE group (p = 0.789). ERA results were available for 158 patients; nonreceptive endometrium was observed in 20.0% of those with CE and 18.9% in those without (p = 0.933).Conclusions: CE was uncommon in patients with infertility and not more prevalent in those with prior implantation failure. CE diagnosis was not associated with BCL6 overexpression or nonreceptive ERA. Routine CE evaluation in the context of failed embryo transfer may contribute to unnecessary interventions.","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144955572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0