Journal of Assisted Reproduction and Genetics最新文献

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Application of a single "Universal warming protocol" for vitrified donor oocytes: A multicenter study.
IF 3.2 3区 医学
Journal of Assisted Reproduction and Genetics Pub Date : 2025-03-28 DOI: 10.1007/s10815-025-03423-7
Paloma Troncoso-Perez, Cristina Gonzalez-Navas, Maria Elisabetta Coccia, Rossella Fucci, Patrizia Falcone, Francesco Bertocci, Rita Picone, Daniel Fernando Sosa-Rosales, Nuria Lopez-Perez, Enrique Criado-Scholz, Miguel Angel Vilches-Ferron
{"title":"Application of a single \"Universal warming protocol\" for vitrified donor oocytes: A multicenter study.","authors":"Paloma Troncoso-Perez, Cristina Gonzalez-Navas, Maria Elisabetta Coccia, Rossella Fucci, Patrizia Falcone, Francesco Bertocci, Rita Picone, Daniel Fernando Sosa-Rosales, Nuria Lopez-Perez, Enrique Criado-Scholz, Miguel Angel Vilches-Ferron","doi":"10.1007/s10815-025-03423-7","DOIUrl":"https://doi.org/10.1007/s10815-025-03423-7","url":null,"abstract":"<p><strong>Purpose: </strong>The objective of the multicenter study is to demonstrate the efficacy of the \"Universal Warming Protocol (UWP)\" using different devitrification media in donor oocytes that were previously vitrified in a single egg bank.</p><p><strong>Method: </strong>A total of 2172 oocytes (metaphase II) were vitrified at Ovobank (Spain) using Kitazato vitrification media (Kitazato, Japan). The oocytes were devitrified at three different centers. Group A: 1021 oocytes devitrified with Kitazato media (Kitazato, Japan); Group B: 496 oocytes devitrified with Irvine media (Fujifilm Irvine Scientific, USA); Group C: 655 oocytes devitrified with RapidWarm Omni media (Vitrolife, Sweden). The study parameters included survival rate, fertilization rate, cleavage rate, blastulation rate, and a pregnancy rate confirmed by a biochemical test; transferring embryos at D + 3 and D + 5; and clinical pregnancy rate, transferring embryos at D + 3 and D + 5.</p><p><strong>Results: </strong>The survival rate was statistically higher in Group C (94%) compared to Group A (86%) and Group B (85%) (P value < 0.01). There were no statistically significant differences in the remaining parameters: fertilization rate (A: 77%, B: 74%, C: 73%); cleavage rate (A: 83%, B: 83%, C: 80%); blastulation rate (A: 52%, B: 58%, C: 57%); a pregnancy rate confirmed by a biochemical test in transferring embryos at D + 3 (A: 38%, B: 33%, C: 33%); a pregnancy rate confirmed by a biochemical test in transferring embryos at D + 5 (A: 54%, B: 76%, C: 65%); clinical pregnancy rate in transferring embryos at D + 3 (A: 27%, B: 13%, C: 18%); clinical pregnancy rate in transferring embryos at D + 5 (A: 39%, B: 57%, C: 60%).</p><p><strong>Conclusions: </strong>The idea that oocyte devitrification medium should always be from the same supplier is questionable. Therefore, it is feasible to use different devitrification media for donor oocytes that were vitrified with the same vitrification medium.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143736025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Up-regulated microRNAs in blastocoel fluid of human implanted embryos could control circuits of pluripotency and be related to embryo competence.
IF 3.2 3区 医学
Journal of Assisted Reproduction and Genetics Pub Date : 2025-03-26 DOI: 10.1007/s10815-025-03457-x
Rosalia Battaglia, Angela Caponnetto, Carmen Ferrara, Anna Fazzio, Cristina Barbagallo, Michele Stella, Davide Barbagallo, Marco Ragusa, Maria Elena Vento, Placido Borzì, Paolo Scollo, Luca Carli, Michael Feichtinger, Evangelia Kasapi, Elias Tsakos, Simone Palini, Wojciech Sierka, Basilio Pecorino, Maria Rosaria Campitiello, Carlo Ronsini, Michele Purrello, Domenico Valerio, Salvatore Longobardi, Thomas D'Hooghe, Cinzia Di Pietro
{"title":"Up-regulated microRNAs in blastocoel fluid of human implanted embryos could control circuits of pluripotency and be related to embryo competence.","authors":"Rosalia Battaglia, Angela Caponnetto, Carmen Ferrara, Anna Fazzio, Cristina Barbagallo, Michele Stella, Davide Barbagallo, Marco Ragusa, Maria Elena Vento, Placido Borzì, Paolo Scollo, Luca Carli, Michael Feichtinger, Evangelia Kasapi, Elias Tsakos, Simone Palini, Wojciech Sierka, Basilio Pecorino, Maria Rosaria Campitiello, Carlo Ronsini, Michele Purrello, Domenico Valerio, Salvatore Longobardi, Thomas D'Hooghe, Cinzia Di Pietro","doi":"10.1007/s10815-025-03457-x","DOIUrl":"https://doi.org/10.1007/s10815-025-03457-x","url":null,"abstract":"<p><strong>Purpose: </strong>The paper aims to investigate the biological role of microRNAs secreted by preimplantation embryo into the blastocoel fluid and to detect a distinctive molecular signature for identifying embryos with the highest implantation potential.</p><p><strong>Methods: </strong>We carried on a multicenter retrospective study involving five European IVF centers. We collected 112 blastocoel fluid samples from embryos on day 5 post-fertilization, cultured individually, along with data on blastocyst grade and embryo transfer outcomes. Using a custom TLDA Array, we compared the expression levels of 89 miRNAs between 33 fluids from high-quality implanted embryos and 30 fluids from high-quality not-implanted embryos. Expression differences were assessed using SAM and t-test. Additionally, correlation and function enrichment analysis and network construction were conducted to identify the biological roles of deregulated microRNAs.</p><p><strong>Results: </strong>We identified six up-regulated microRNAs in the blastocoel fluid from implanted embryos, significantly and positively correlated across all samples (r ≥ 0.7; P ≤ 0.05). They could take part in pluripotency circuits, regulating and being regulated by transcription factors associated with stemness, cell growth, and embryo development. The ROC curve analysis confirmed the potential of these miRNAs as implantation classifiers.</p><p><strong>Conclusion: </strong>The six miRNAs up-regulated in blastocoel fluid from implanted embryos may represent a functional molecular signature for evaluating blastocyst quality and identifying the most competent embryos. Their evaluation associated with non-invasive preimplantation genetic testing, integrating epigenetic and genomic analyses, could enhance implantation grade and allow for identification of the euploid embryo not able to implant.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143719385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Abnormal sperm tails correlate with the sperm DNA fragmentation index, irrespective of progressive motility, according to an analysis of 5125 semen reports.
IF 3.2 3区 医学
Journal of Assisted Reproduction and Genetics Pub Date : 2025-03-25 DOI: 10.1007/s10815-025-03452-2
Yanjing Wang, Hongping Li, Minhao Hu, Xiaozhen Liu, Lejun Li
{"title":"Abnormal sperm tails correlate with the sperm DNA fragmentation index, irrespective of progressive motility, according to an analysis of 5125 semen reports.","authors":"Yanjing Wang, Hongping Li, Minhao Hu, Xiaozhen Liu, Lejun Li","doi":"10.1007/s10815-025-03452-2","DOIUrl":"https://doi.org/10.1007/s10815-025-03452-2","url":null,"abstract":"<p><strong>Purpose: </strong>Sperm progressive motility (PR) is correlated with the sperm DNA fragmentation index (SDF). Moreover, abnormalities in sperm tails are closely associated with the quality of sperm PR. However, the correlation of abnormal sperm tails with the SDF remains unknown.</p><p><strong>Methods: </strong>Routine semen examination was performed according to the World Health Organization guidelines with a computer-assisted semen analysis system. SDF analysis was conducted via the sperm chromatin structure assay method. Spearman correlation was used to test the correlation of the SDF with age and conventional semen parameters. The relationship between the SDF and abnormal tails was explored by binary logistic regression analysis, restricted cubic spline (RCS) analyses and subgroup analysis. ROC curves were used to assess the predictive capacity of age, abnormal tails and PR for the SDF.</p><p><strong>Results: </strong>The SDF was associated with sperm morphology, with a significant correlation observed with abnormal sperm tails (r = 0.491, P < 0.001). Binary regression analysis revealed that abnormal sperm tails were an independent influencing factor of the SDF. RCS analyses and Trend analysis suggested that as the number of abnormal sperm tails increased, the risk of an abnormal SDF also increased, and the trend was statistically significant (P for trend < 0.001). The ROC curve demonstrated that abnormal sperm tails had good discriminatory ability for the SDF (AUC: 0.757, P < 0.001).</p><p><strong>Conclusion: </strong>Abnormal sperm tails are an independent influencing factor of the SDF, and a higher number of abnormal tails is associated with an increased risk of an abnormal SDF.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143709967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Association of endometrial preparation protocols and embryonic development stages with ectopic pregnancy in freeze-thaw cycles.
IF 3.2 3区 医学
Journal of Assisted Reproduction and Genetics Pub Date : 2025-03-25 DOI: 10.1007/s10815-025-03451-3
Jinliang Zhu, Wan Yang, Ming Li, Xinjie Zhuang, Yapeng Wang, Ying Lian, Rong Li, Ping Liu
{"title":"Association of endometrial preparation protocols and embryonic development stages with ectopic pregnancy in freeze-thaw cycles.","authors":"Jinliang Zhu, Wan Yang, Ming Li, Xinjie Zhuang, Yapeng Wang, Ying Lian, Rong Li, Ping Liu","doi":"10.1007/s10815-025-03451-3","DOIUrl":"https://doi.org/10.1007/s10815-025-03451-3","url":null,"abstract":"<p><strong>Purpose: </strong>To investigate the correlation between endometrial preparation protocols, embryonic development stages, and ectopic pregnancy.</p><p><strong>Methods: </strong>A total of 29,700 clinical pregnancies, all of which resulted from freeze-thaw cycles performed between January 2018 and December 2023, were analyzed in this retrospective study.</p><p><strong>Results: </strong>The ectopic pregnancy rate was significantly higher in artificial cycles than natural cycles (1.5% vs. 0.8%; P < 0.001); the ectopic pregnancy rate was significantly higher in stage 5-6 than stage 3-4 blastocyst transfer (1.3% vs. 0.7%; P = 0.019). After incorporating all independent confounders into a multivariate general estimating equation model, artificial versus natural cycle (adjusted RR = 1.68, 95% CI = 1.32-2.13; P < 0.001) was shown to be an increased risk factor for ectopic pregnancy, especially in the single embryo transfer subgroup (aRR = 1.75, 95% CI = 1.23-2.48; P = 0.002); additionally, day 3 embryo transfer versus blastocyst transfer (aRR = 1.97, 95% CI = 1.15-3.38; P = 0.014) and stage 5-6 blastocyst versus stage 3-4 blastocyst transfer (aRR = 1.70, 95% CI = 1.05-2.74; P = 0.031) were shown to be increased risk factors for ectopic pregnancy.</p><p><strong>Conclusions: </strong>This study found that endometrial preparation protocols and embryonic development stages are significantly associated with ectopic pregnancy.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143709407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Personalized prediction model for miscarriage: in-depth sperm DNA fragmentation.
IF 3.2 3区 医学
Journal of Assisted Reproduction and Genetics Pub Date : 2025-03-25 DOI: 10.1007/s10815-025-03445-1
Chun-I Lee, Tse-En Wang, Chien-Hong Chen, Chun-Chia Huang, Hui-Mei Tsao, Tsung-Hsien Lee, Tiencheng Arthur Chang, Cheng-Teng Hsu, Maw-Sheng Lee
{"title":"Personalized prediction model for miscarriage: in-depth sperm DNA fragmentation.","authors":"Chun-I Lee, Tse-En Wang, Chien-Hong Chen, Chun-Chia Huang, Hui-Mei Tsao, Tsung-Hsien Lee, Tiencheng Arthur Chang, Cheng-Teng Hsu, Maw-Sheng Lee","doi":"10.1007/s10815-025-03445-1","DOIUrl":"https://doi.org/10.1007/s10815-025-03445-1","url":null,"abstract":"<p><strong>Purpose: </strong>We investigated how different types of sperm DNA fragmentation (SDF) in males, in conjunction with varying levels of female reproductive potential, jointly impact ICSI outcomes.</p><p><strong>Methods: </strong>We retrospectively analyzed 195 couples undergoing ICSI, categorizing them by normal or poor prognosis according to POSEIDON criteria. Female factors included age, anti-Müllerian hormone (AMH), and oocyte retrieval numbers. Male factors included semen parameters, total SDF, and specific double-strand breaks (DSBs). Reproductive outcomes were followed up at different gestational stages, including clinical pregnancy, early gestation failure, live birth, and miscarriage. Risk factors were identified using univariate and multivariable logistic regression, and their predictive power was assessed via the receiver operating characteristic (ROC) curve.</p><p><strong>Results: </strong>In the normal group, female factors were primarily associated with reproductive failures. Non-pregnancy cases had lower AMH (4 ng/mL vs. 3.2 ng/mL), and miscarriage cases had fewer oocytes retrieved (15 vs. 10.5). However, the risk factor profile was distinct in poor prognosis. Male factors, including reduced sperm motility (68% vs. 54.5%), lower normal sperm morphology (5.5% vs. 2.5%), and elevated DSBs (7.5% vs. 18.8%) were linked to miscarriage. DSBs presented as the independent predictor of miscarriage risk (odds ratio: 1.19, 95% CI: 1.04-1.36), with a DSB cutoff of 19% providing 81% accuracy in predicting miscarriage.</p><p><strong>Conclusion: </strong>Paternal effect is pronounced in women with poor prognosis, where elevated DSBs are linked to an increased risk of miscarriage. We propose a refined pipeline in which DSB testing is considered as initial evaluation before assisted reproductive treatments, especially for infertile couples with poor prognosis.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143709417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Response to: Critical appraisal of "pregnancy outcomes in patients with non-obstructive azoospermia undergoing micro-TESE: comparison of fresh vs. frozen-thawed testicular sperm".
IF 3.2 3区 医学
Journal of Assisted Reproduction and Genetics Pub Date : 2025-03-25 DOI: 10.1007/s10815-025-03458-w
Lala Suleymanova, Hale Bayram, Yaprak Dönmez Çakıl, Belgin Selam, Mehmet Cıncık
{"title":"Response to: Critical appraisal of \"pregnancy outcomes in patients with non-obstructive azoospermia undergoing micro-TESE: comparison of fresh vs. frozen-thawed testicular sperm\".","authors":"Lala Suleymanova, Hale Bayram, Yaprak Dönmez Çakıl, Belgin Selam, Mehmet Cıncık","doi":"10.1007/s10815-025-03458-w","DOIUrl":"https://doi.org/10.1007/s10815-025-03458-w","url":null,"abstract":"","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143709569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Incidence of cross-border reproductive care and pregnancy outcomes: a retrospective evaluation of IVF conceptions. 跨境生殖护理的发生率和妊娠结果:对试管婴儿受孕的回顾性评估。
IF 3.2 3区 医学
Journal of Assisted Reproduction and Genetics Pub Date : 2025-03-22 DOI: 10.1007/s10815-025-03449-x
Suset Rodriguez, Kyara Marquez, Gemma St Louis, Arianna Vazquez, Leonardo Simonelli, Kara Lindsay, Janice Moscoso, George Roshdy Attia
{"title":"Incidence of cross-border reproductive care and pregnancy outcomes: a retrospective evaluation of IVF conceptions.","authors":"Suset Rodriguez, Kyara Marquez, Gemma St Louis, Arianna Vazquez, Leonardo Simonelli, Kara Lindsay, Janice Moscoso, George Roshdy Attia","doi":"10.1007/s10815-025-03449-x","DOIUrl":"https://doi.org/10.1007/s10815-025-03449-x","url":null,"abstract":"<p><strong>Purpose: </strong>This study aimed to evaluate the incidence of cross-border reproductive care (CBRC) and maternal and neonatal outcomes in pregnancies conceived through in vitro fertilization (IVF), comparing outcomes between pregnancies conceived domestically and through CBRC.</p><p><strong>Methods: </strong>A retrospective chart review was conducted on 4475 deliveries at a tertiary public hospital from February 1, 2023, to January 31, 2024. Maternal demographics, medical history, delivery outcomes, and complications were compared using statistical tests, with significance set at p < 0.05.</p><p><strong>Results: </strong>Among all deliveries, 95 were conceived via IVF. Among the IVF pregnancies, 23 (24.2%) were conceived through CBRC. While CBRC patients were older on average (40.3 vs. 37.8 years), this difference was not statistically significant (p = 0.07). The incidence of hypertensive disorders was significantly higher in CBRC pregnancies compared to domestic IVF pregnancies (69.6% vs. 37.7%, p = 0.01). Trends toward increased rates of postpartum hemorrhage (17.4% vs. 5.8%) and multiple gestations (26.1% vs. 11.6%) were also observed in the CBRC group, although these differences did not reach statistical significance. Neonatal outcomes, including rates of preterm birth, fetal growth restriction, and respiratory distress syndrome, were similar between the two groups.</p><p><strong>Conclusion: </strong>The study highlights a significantly higher percentage of hypertensive disorders among CBRC pregnancies, while other maternal and neonatal outcomes were comparable to domestic IVF pregnancies. These findings underscore the need for further research with larger sample sizes and survey-based studies to better understand patient motivations and clinical risks associated with CBRC.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143677009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
m6A RNA methylation dynamics during in vitro maturation of cumulus-oocyte complexes derived from adult or prepubertal sheep.
IF 3.2 3区 医学
Journal of Assisted Reproduction and Genetics Pub Date : 2025-03-18 DOI: 10.1007/s10815-025-03444-2
Chiara Cosseddu, Sara Succu, Adele Frau, Francesca Mossa, Sylvia Virginie Versace, Tiziana A L Brevini, Sergio Ledda, Daniela Bebbere
{"title":"m6A RNA methylation dynamics during in vitro maturation of cumulus-oocyte complexes derived from adult or prepubertal sheep.","authors":"Chiara Cosseddu, Sara Succu, Adele Frau, Francesca Mossa, Sylvia Virginie Versace, Tiziana A L Brevini, Sergio Ledda, Daniela Bebbere","doi":"10.1007/s10815-025-03444-2","DOIUrl":"https://doi.org/10.1007/s10815-025-03444-2","url":null,"abstract":"<p><strong>Purpose: </strong>N6-methyladenosine (m6A) is the most prevalent base epigenetic modification within eukaryotic mRNAs. It participates in post-transcriptional regulation, including maternal RNA maintenance and decay in mouse oocytes and during maternal-to-zygotic transition. The landscape in other mammalian species remains largely unexplored. The present work analyzed m6A dynamics in sheep cumulus oocyte complexes (COCs), during in vitro maturation. To explore potential relationships with oocyte developmental competence, a previously established model consisting of oocytes derived from adult and prepubertal sheep was adopted.</p><p><strong>Methods: </strong>m6a dynamics were analyzed in terms of m6A RNA methylation abundance in cumulus cells (CCs) by colorimetric assay and expression of key m6A methylation-related proteins (METTL3, METTL14, METTL16, VIRMA, YTHDC1, YTHDC2, YTHDF2, YTHDF3, ALKBH5, and FTO) in both cumulus cells and oocytes by real-time PCR.</p><p><strong>Results: </strong>We report the dynamics of m6A in sheep COCs, and reveal alterations in both oocytes and cumulus cells derived from prepubertal donors. These changes were observed in terms of m6A RNA methylation levels and transcript dynamics of several m6A methylation-related proteins. Notably, our study shows that dysregulations occur after IVM.</p><p><strong>Conclusion: </strong>Overall, this work describes for the first time the dynamics of m6A in sheep COCs and uncovers the involvement of m6A RNA methylation in oocyte developmental potential.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143648608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Infertility management in a patient with Swyer syndrome: a case report. 斯韦尔综合征患者的不孕症治疗:病例报告。
IF 3.2 3区 医学
Journal of Assisted Reproduction and Genetics Pub Date : 2025-03-18 DOI: 10.1007/s10815-025-03442-4
Laura Krygere, Ruta Bartasiene, Agne Kozlovskaja-Gumbriene, Egle Drejeriene
{"title":"Infertility management in a patient with Swyer syndrome: a case report.","authors":"Laura Krygere, Ruta Bartasiene, Agne Kozlovskaja-Gumbriene, Egle Drejeriene","doi":"10.1007/s10815-025-03442-4","DOIUrl":"https://doi.org/10.1007/s10815-025-03442-4","url":null,"abstract":"<p><strong>Purpose: </strong>To highlight the successful use of assisted reproductive technologies in achieving pregnancy and healthy childbirth in a woman with Swyer syndrome, a rare disorder of sex development characterized by gonadal dysgenesis, elevated gonadotropin levels, oestrogen insufficiency, tall stature, delayed puberty, and primary amenorrhoea. This case highlights the efficacy of reproductive treatments for individuals with nonfunctional gonads resulting from complete gonadal dysgenesis.</p><p><strong>Methods: </strong>This report describes a woman diagnosed with Swyer syndrome at 18 years, presenting with primary amenorrhoea and delayed puberty. Following diagnosis, the patient underwent bilateral gonadectomy due to the increased risk of germ cell neoplasia and began hormone replacement therapy to address oestrogen insufficiency. At 31, the patient pursued fertility treatment, undergoing in vitro fertilisation (IVF) with donated oocytes.</p><p><strong>Results: </strong>The IVF treatment using donor oocytes led to a successful pregnancy without complications. The pregnancy culminated in the delivery of a healthy baby via caesarean section, marking a successful outcome for a patient with Swyer syndrome.</p><p><strong>Conclusion: </strong>Assisted reproductive technologies, particularly in vitro fertilisation (IVF) using oocyte donation, provide a feasible option for individuals diagnosed with Swyer syndrome to achieve parenthood. This case adds to the growing research on reproductive success in women with complete gonadal dysgenesis, emphasizing the potential for positive outcomes through specific reproductive approaches.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143648596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Mitochondrial FIS1 level in cumulus cells correlates with morphological grades of human cleavage-stage embryos. 积液细胞中线粒体 FIS1 的水平与人类分裂期胚胎的形态等级相关。
IF 3.2 3区 医学
Journal of Assisted Reproduction and Genetics Pub Date : 2025-03-17 DOI: 10.1007/s10815-025-03431-7
Yizhen Sima, Sanbao Shi, Zhunyuan Min, Yuning Chen, Yongning Lu, Hongying Sha, Suying Liu
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