Journal of Assisted Reproduction and Genetics最新文献

{"title":"Ovulatory cycle frozen embryo transfer yields similar pregnancy outcomes to programmed transfer while avoiding painful injections.","authors":"Katie White, Jessica Cheung, Prachi Godiwala, Reeva Makhijani, Allison Bartolucci, Claudio Benadiva, Lawrence Engmann, Daniel Grow","doi":"10.1007/s10815-025-03446-0","DOIUrl":"https://doi.org/10.1007/s10815-025-03446-0","url":null,"abstract":"<p><strong>Purpose: </strong>Optimal endometrial preparation is essential for the transfer of a euploid embryo. Recent works have shown advantages of ovulatory cycle frozen-thawed embryo transfer (Ovu-FET) for simplicity, implantation rate, and reduction of hypertensive disorders of pregnancy; however, few women over age 40 years were included in these studies. This study compared pregnancy outcomes in patients aged 40-45 years between Ovu-FET and programmed cycle FET (PC-FET).</p><p><strong>Methods: </strong>Retrospective cohort study in a university-affiliated fertility center. Two hundred eighty-seven FET cycles with euploid embryo transfer between 2015 and 2022 were included, with 116 cycles utilizing the PC-FET and 171 the Ovu-FET protocol. The use of letrozole was permitted for patients with irregular cycles. The primary outcome was ongoing pregnancy rate with secondary outcomes including pregnancy rate, clinical pregnancy rate, biochemical rate, and clinical loss rate. Multivariate regression was utilized to adjust for potential covariates.</p><p><strong>Results: </strong>The ongoing pregnancy rates were similar between the Ovu-FET versus PC-FET groups (62.6% vs. 58.6%, P = 0.50). Pregnancy rates (77.2% vs. 82.8%, P = 0.25) and clinical pregnancy rates (69.5% vs. 68.1%, P = 0.79) were also similar. Clinical loss rates were similar between the two groups (7.0% vs. 9.5%, P = 0.41). Biochemical loss rates were slightly lower for the Ovu-FET versus PC-FET groups (7.6% vs. 14.7%, P = 0.06) without reaching statistical significance.</p><p><strong>Conclusion: </strong>Our study supports the use of Ovu-FET in women aged 40 years and older undergoing euploid embryo transfers. The results should strengthen confidence in the use of Ovu-FET for older patients.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143752945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fertility preservation in female cancer patients: assessing awareness, attitudes, concerns, experiences, and the role of counseling in quality of life.","authors":"Eman Sbaity, Nagham Ramadan, Ali Slim, Tasnim Diab, Houry Kazarian, Ali Tarhini, Maya Charafeddine, Ghina Ghazeeri, Ali Bazarbachi, Jean El Cheikh, Nagi S El Saghir, Hazem I Assi","doi":"10.1007/s10815-025-03461-1","DOIUrl":"https://doi.org/10.1007/s10815-025-03461-1","url":null,"abstract":"<p><strong>Background: </strong>Cancer poses significant fertility challenges for women of childbearing age, yet the adoption of fertility preservation (FP) measures remains limited. This study aimed to explore patients' understanding, attitudes, concerns, psychological well-being, and factors influencing their decisions regarding FP during treatment.</p><p><strong>Methods: </strong>This prospective cohort study involved women aged 18 to 42 diagnosed with non-metastatic breast cancer or lymphoma, treated at the American University of Beirut Medical Center. Patients completed questionnaires at intervals over a 2- to 3-year period following diagnosis.</p><p><strong>Results: </strong>Among the 123 women studied, 71.5% were married, and 13.5% had no children. Although 87.8% were informed about the fertility risks of their treatment, only 45.4% expressed a desire for FP. Multivariable analysis revealed that parity was significantly associated with fertility concerns (p = 0.019). Decisions favoring FP were significantly influenced by younger age at diagnosis, lower parity, and higher education levels (p = 0.001, p < 0.001, and p = 0.036, respectively). While 56% of patients received FP counseling-mostly from oncologists (83%)-the preference for FP methods varied, with 47% choosing medical approaches and 53% opting for procedural interventions. Despite recognizing the importance of FP, 91% reported that it did not significantly influence their treatment choices. Patients showed greater satisfaction when oncologists discussed fertility-related topics (p < 0.001). Over time, overall FertiQoL scores and DASS-42 scores indicated improved psychological well-being.</p><p><strong>Conclusion: </strong>Our study highlights the complexities of FP decisions among cancer survivors, emphasizing the need for proper counseling to support their quality of life and psychological well-being.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143752942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlation analysis of a novel artificial intelligence optical microscope-assisted semen assessment system with IVF outcomes.","authors":"Shiqi Yi, Li Yang, Yihong Yang, Fan Wei, Xiao Zhun, Yan Wang","doi":"10.1007/s10815-025-03453-1","DOIUrl":"https://doi.org/10.1007/s10815-025-03453-1","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Purpose: &lt;/strong&gt;On the day of fresh oocyte retrieval in in vitro fertilization (IVF) cycles, a novel portable artificial intelligence optical microscope (AIOM) was employed to assist in the assessment of semen parameters. This study analyzed the correlation between sperm kinetic and morphological parameters with short-term IVF outcomes. Additionally, it explored whether these parameters could serve as predictive indicators for rescue intracytoplasmic sperm injection (R-ICSI) in IVF patients.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;A retrospective analysis was conducted on patients undergoing short-term IVF at the West China Second Hospital of Sichuan University between May 2021 and May 2024. Based on fertilization outcomes, the short-term IVF patients were categorized into a successful fertilization group (group A, n = 281) and a group requiring R-ICSI after failed fertilization (group B, n = 49). AIOM was utilized to analyze semen parameters including pH, sperm concentration, sperm motility parameters, sperm movement trajectory parameters, and sperm morphological parameters. The study further investigated the correlation between these short-term IVF fertilization-related laboratory indicators and IVF outcomes.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;No statistically significant difference was observed in semen pH between the two groups. However, there were significant differences in sperm concentration and the majority of motility parameters. Specifically, compared to group A, patients in group B exhibited lower sperm concentration (p = 0.01), motility (p = 0.01), local motility (p = 0.01), progressive motility (PR) (p = 0.00), total motility (p = 0.01), and amplitude of lateral head displacement (ALH) (p &lt; 0.01), along with higher immotility (p = 0.00). No statistically significant differences were found between the two groups in other sperm motility, velocity, or trajectory parameters. Additionally, sperm morphological parameters were also associated with short-term IVF fertilization outcomes. Compared to group A, group B had higher sperm head length mean (p &lt; 0.01), head perimeter mean (p &lt; 0.01), and head area mean (p = 0.01), as well as lower tail length mean (p = 0.01). Multivariate regression analysis of fertilization outcomes indicated that higher immotility (p = 0.01) and head length mean (p &lt; 0.01), along with lower tail length mean (p = 0.04), were independent risk factors affecting successful short-term IVF fertilization. Notably, head length mean showed a significant negative correlation with polyspermy rate (p &lt; 0.01), whereas tail length mean was significantly positively correlated with polyspermy rate (p &lt; 0.01).&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusion: &lt;/strong&gt;Optimization of semen parameters with AIOM at the time of fertilization is significantly associated with short-term IVF fertilization outcomes. Abnormal semen parameters at fertilization-specifically, higher immotility and head length mean, along with lower tail length mean-can be consider","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143752940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Changing sperm donors-a shortcut to pregnancy or just a myth?","authors":"Shimi Barda, Yael Eliner, Noga Fuchs Weizman, Hadar Amir, Sandra E Kleiman, Foad Azem, Ron Hauser","doi":"10.1007/s10815-025-03464-y","DOIUrl":"https://doi.org/10.1007/s10815-025-03464-y","url":null,"abstract":"<p><strong>Purpose: </strong>Changing sperm donors after unsuccessful intrauterine insemination (IUI) cycles is a common yet understudied practice. This study evaluates whether switching sperm donors impacts the number of IUI cycles required to achieve pregnancy.</p><p><strong>Methods: </strong>This retrospective cohort study analyzed 312 women undergoing donor sperm IUI at Lis Maternity Hospital, Tel Aviv Sourasky Medical Center, from 1992 to 2020. Participants were divided into two groups: Group A (conceived using only one donor) and Group B (switched donors after initial unsuccessful attempts). The primary outcome was the number of IUI cycles until pregnancy. Statistical analyses included t-tests, ANOVA, and multivariate analysis of covariance (MANCOVA).</p><p><strong>Results: </strong>Women in Group A required fewer cycles (mean 3.78 ± 1.90) to achieve pregnancy compared to Group B (mean 6.07 ± 2.95, P < .001). However, after switching donors, the mean number of cycles needed in Group B (2.23 ± 1.61) was significantly lower than the total cycles required by Group A (P < .001). Cumulative live birth rates were higher in Group A (50.5% after three cycles; 81.5% after six cycles) compared to Group B (26.0% after three cycles; 61.9% after six cycles).</p><p><strong>Conclusion: </strong>Switching sperm donors after repeated unsuccessful IUI attempts significantly reduces the additional number of cycles needed to achieve pregnancy. These findings suggest that sperm-oocyte compatibility may influence IUI success. Clinicians should consider donor replacement after multiple failures. Prospective studies are required to confirm these results and investigate underlying biological mechanisms.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143752935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of low versus high oxygen tension on human oocyte maturation during biphasic capacitation IVM (CAPA-IVM).","authors":"Huy H Pham, Viet Q Tran, Anh H Le, Duy L Nguyen, Toan D Pham, Anh L Vu, Tien K Le, Ho L Le, Bao G Huynh, Tuong M Ho, Lan N Vuong","doi":"10.1007/s10815-025-03459-9","DOIUrl":"https://doi.org/10.1007/s10815-025-03459-9","url":null,"abstract":"<p><strong>Purpose: </strong>This study investigated the impact of low versus high oxygen tension on the oocyte maturation rate in biphasic CAPA-IVM.</p><p><strong>Methods: </strong>A sibling oocyte pilot study of cumulus-oocyte complexes (COCs) from participants with polycystic ovary syndrome was performed at an academic IVF center in Vietnam from November 2023 to May 2024. At collection, COCs were allocated to undergo CAPA-IVM culture with low oxygen tension (5%) or high oxygen tension (20%). Culture of COCs took place in two benchtop incubators, each equipped with commercial mix-gas bottles to establish the respective oxygen conditions. The primary outcome was the oocyte maturation rate. Secondary outcomes were rates of two-pronuclei fertilization, day-3 embryos, blastocyst formation, and good-quality embryos.</p><p><strong>Results: </strong>A total of 554 COCs from 20 participants were assigned to the low oxygen (276 COCs) or high oxygen (278 COCs) tension groups. The oocyte maturation rate was significantly lower in the low oxygen tension versus high oxygen tension group (53.6% vs. 65.8%, risk ratio 0.81, 95% confidence interval 0.71-0.94; p=0.004). The two-pronuclei fertilization rate was significantly lower in the low oxygen tension versus high oxygen tension group (61.5% vs. 72.7%, p=0.03). Numbers of day-3 embryos, blastocysts, and good-quality blastocysts were slightly, but not significantly, lower in the low oxygen tension group compared with the high oxygen tension group.</p><p><strong>Conclusions: </strong>Low oxygen tension of 5% during both steps of biphasic CAPA-IVM reduced the number of matured oocytes and 2-pronuclei fertilized oocytes compared with the use of high oxygen tension (20%) during CAPA-IVM.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143742896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between hatching status and pregnancy outcomes in single blastocyst transfers: a retrospective cohort analysis.","authors":"Weimin Yang, Qingkai Wang, Bin Zhang, Ross Ka-Kit Leung, Kai Deng, Shuangshuang Geng, Jinfeng Xu, Yu Qiao, Hui Gao, Dongchuan Li, Liyi Cai","doi":"10.1007/s10815-025-03450-4","DOIUrl":"https://doi.org/10.1007/s10815-025-03450-4","url":null,"abstract":"<p><strong>Objective: </strong>To examine the potential association between blastocyst hatching status and pregnancy outcomes following single blastocyst transfer.</p><p><strong>Methods: </strong>This is a retrospective cohort. We screened all frozen-thawed single blastocyst transfer cycles from January 1, 2020, to April 30, 2022, at the authors' center. The hatching status was graded into four categories: unhatched, early hatching (hatched portion < the diameter of zona pellucida), late hatching (hatched portion > the diameter of zona pellucida), and fully hatched. Multivariate logistic regression was used to examine the association between hatching status and pregnancy outcomes (clinical pregnancy and live birth).</p><p><strong>Results: </strong>The final analysis included 906 cycles. The hatching status was unhatched in 116 cycles, early hatching in 556 cycles, late hatching in 197 cycles, and fully hatched in 37 cycles. The clinical pregnancy rate was 54.3%, 63.3%, 74.6%, and 54.1%, respectively (p = 0.001) in the unhatched, early-hatching, late-hatching, and fully hatched groups, respectively. The live birth rate was 39.7%, 51.6%, 58.3%, and 40.5%, respectively (p = 0.008). In pairwise comparisons, the late-hatching group had significantly higher rates of clinical pregnancy and live birth versus the unhatched category (p = 0.001 and p = 0.008, respectively). In multivariate logistic regression analysis, embryo hatching status, the duration until blastocyst formation, the grading of blastocyst cells, and the thickness of the endometrium were associated with clinical pregnancy and live birth.</p><p><strong>Conclusion: </strong>After adjusting for confounding factors, late-hatching status of the blastocysts was associated with a higher rate of clinical pregnancy and live birth.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143736045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of a single \"Universal warming protocol\" for vitrified donor oocytes: A multicenter study.","authors":"Paloma Troncoso-Perez, Cristina Gonzalez-Navas, Maria Elisabetta Coccia, Rossella Fucci, Patrizia Falcone, Francesco Bertocci, Rita Picone, Daniel Fernando Sosa-Rosales, Nuria Lopez-Perez, Enrique Criado-Scholz, Miguel Angel Vilches-Ferron","doi":"10.1007/s10815-025-03423-7","DOIUrl":"https://doi.org/10.1007/s10815-025-03423-7","url":null,"abstract":"<p><strong>Purpose: </strong>The objective of the multicenter study is to demonstrate the efficacy of the \"Universal Warming Protocol (UWP)\" using different devitrification media in donor oocytes that were previously vitrified in a single egg bank.</p><p><strong>Method: </strong>A total of 2172 oocytes (metaphase II) were vitrified at Ovobank (Spain) using Kitazato vitrification media (Kitazato, Japan). The oocytes were devitrified at three different centers. Group A: 1021 oocytes devitrified with Kitazato media (Kitazato, Japan); Group B: 496 oocytes devitrified with Irvine media (Fujifilm Irvine Scientific, USA); Group C: 655 oocytes devitrified with RapidWarm Omni media (Vitrolife, Sweden). The study parameters included survival rate, fertilization rate, cleavage rate, blastulation rate, and a pregnancy rate confirmed by a biochemical test; transferring embryos at D + 3 and D + 5; and clinical pregnancy rate, transferring embryos at D + 3 and D + 5.</p><p><strong>Results: </strong>The survival rate was statistically higher in Group C (94%) compared to Group A (86%) and Group B (85%) (P value < 0.01). There were no statistically significant differences in the remaining parameters: fertilization rate (A: 77%, B: 74%, C: 73%); cleavage rate (A: 83%, B: 83%, C: 80%); blastulation rate (A: 52%, B: 58%, C: 57%); a pregnancy rate confirmed by a biochemical test in transferring embryos at D + 3 (A: 38%, B: 33%, C: 33%); a pregnancy rate confirmed by a biochemical test in transferring embryos at D + 5 (A: 54%, B: 76%, C: 65%); clinical pregnancy rate in transferring embryos at D + 3 (A: 27%, B: 13%, C: 18%); clinical pregnancy rate in transferring embryos at D + 5 (A: 39%, B: 57%, C: 60%).</p><p><strong>Conclusions: </strong>The idea that oocyte devitrification medium should always be from the same supplier is questionable. Therefore, it is feasible to use different devitrification media for donor oocytes that were vitrified with the same vitrification medium.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143736025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Up-regulated microRNAs in blastocoel fluid of human implanted embryos could control circuits of pluripotency and be related to embryo competence.","authors":"Rosalia Battaglia, Angela Caponnetto, Carmen Ferrara, Anna Fazzio, Cristina Barbagallo, Michele Stella, Davide Barbagallo, Marco Ragusa, Maria Elena Vento, Placido Borzì, Paolo Scollo, Luca Carli, Michael Feichtinger, Evangelia Kasapi, Elias Tsakos, Simone Palini, Wojciech Sierka, Basilio Pecorino, Maria Rosaria Campitiello, Carlo Ronsini, Michele Purrello, Domenico Valerio, Salvatore Longobardi, Thomas D'Hooghe, Cinzia Di Pietro","doi":"10.1007/s10815-025-03457-x","DOIUrl":"https://doi.org/10.1007/s10815-025-03457-x","url":null,"abstract":"<p><strong>Purpose: </strong>The paper aims to investigate the biological role of microRNAs secreted by preimplantation embryo into the blastocoel fluid and to detect a distinctive molecular signature for identifying embryos with the highest implantation potential.</p><p><strong>Methods: </strong>We carried on a multicenter retrospective study involving five European IVF centers. We collected 112 blastocoel fluid samples from embryos on day 5 post-fertilization, cultured individually, along with data on blastocyst grade and embryo transfer outcomes. Using a custom TLDA Array, we compared the expression levels of 89 miRNAs between 33 fluids from high-quality implanted embryos and 30 fluids from high-quality not-implanted embryos. Expression differences were assessed using SAM and t-test. Additionally, correlation and function enrichment analysis and network construction were conducted to identify the biological roles of deregulated microRNAs.</p><p><strong>Results: </strong>We identified six up-regulated microRNAs in the blastocoel fluid from implanted embryos, significantly and positively correlated across all samples (r ≥ 0.7; P ≤ 0.05). They could take part in pluripotency circuits, regulating and being regulated by transcription factors associated with stemness, cell growth, and embryo development. The ROC curve analysis confirmed the potential of these miRNAs as implantation classifiers.</p><p><strong>Conclusion: </strong>The six miRNAs up-regulated in blastocoel fluid from implanted embryos may represent a functional molecular signature for evaluating blastocyst quality and identifying the most competent embryos. Their evaluation associated with non-invasive preimplantation genetic testing, integrating epigenetic and genomic analyses, could enhance implantation grade and allow for identification of the euploid embryo not able to implant.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143719385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Abnormal sperm tails correlate with the sperm DNA fragmentation index, irrespective of progressive motility, according to an analysis of 5125 semen reports.","authors":"Yanjing Wang, Hongping Li, Minhao Hu, Xiaozhen Liu, Lejun Li","doi":"10.1007/s10815-025-03452-2","DOIUrl":"https://doi.org/10.1007/s10815-025-03452-2","url":null,"abstract":"<p><strong>Purpose: </strong>Sperm progressive motility (PR) is correlated with the sperm DNA fragmentation index (SDF). Moreover, abnormalities in sperm tails are closely associated with the quality of sperm PR. However, the correlation of abnormal sperm tails with the SDF remains unknown.</p><p><strong>Methods: </strong>Routine semen examination was performed according to the World Health Organization guidelines with a computer-assisted semen analysis system. SDF analysis was conducted via the sperm chromatin structure assay method. Spearman correlation was used to test the correlation of the SDF with age and conventional semen parameters. The relationship between the SDF and abnormal tails was explored by binary logistic regression analysis, restricted cubic spline (RCS) analyses and subgroup analysis. ROC curves were used to assess the predictive capacity of age, abnormal tails and PR for the SDF.</p><p><strong>Results: </strong>The SDF was associated with sperm morphology, with a significant correlation observed with abnormal sperm tails (r = 0.491, P < 0.001). Binary regression analysis revealed that abnormal sperm tails were an independent influencing factor of the SDF. RCS analyses and Trend analysis suggested that as the number of abnormal sperm tails increased, the risk of an abnormal SDF also increased, and the trend was statistically significant (P for trend < 0.001). The ROC curve demonstrated that abnormal sperm tails had good discriminatory ability for the SDF (AUC: 0.757, P < 0.001).</p><p><strong>Conclusion: </strong>Abnormal sperm tails are an independent influencing factor of the SDF, and a higher number of abnormal tails is associated with an increased risk of an abnormal SDF.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143709967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of endometrial preparation protocols and embryonic development stages with ectopic pregnancy in freeze-thaw cycles.","authors":"Jinliang Zhu, Wan Yang, Ming Li, Xinjie Zhuang, Yapeng Wang, Ying Lian, Rong Li, Ping Liu","doi":"10.1007/s10815-025-03451-3","DOIUrl":"https://doi.org/10.1007/s10815-025-03451-3","url":null,"abstract":"<p><strong>Purpose: </strong>To investigate the correlation between endometrial preparation protocols, embryonic development stages, and ectopic pregnancy.</p><p><strong>Methods: </strong>A total of 29,700 clinical pregnancies, all of which resulted from freeze-thaw cycles performed between January 2018 and December 2023, were analyzed in this retrospective study.</p><p><strong>Results: </strong>The ectopic pregnancy rate was significantly higher in artificial cycles than natural cycles (1.5% vs. 0.8%; P < 0.001); the ectopic pregnancy rate was significantly higher in stage 5-6 than stage 3-4 blastocyst transfer (1.3% vs. 0.7%; P = 0.019). After incorporating all independent confounders into a multivariate general estimating equation model, artificial versus natural cycle (adjusted RR = 1.68, 95% CI = 1.32-2.13; P < 0.001) was shown to be an increased risk factor for ectopic pregnancy, especially in the single embryo transfer subgroup (aRR = 1.75, 95% CI = 1.23-2.48; P = 0.002); additionally, day 3 embryo transfer versus blastocyst transfer (aRR = 1.97, 95% CI = 1.15-3.38; P = 0.014) and stage 5-6 blastocyst versus stage 3-4 blastocyst transfer (aRR = 1.70, 95% CI = 1.05-2.74; P = 0.031) were shown to be increased risk factors for ectopic pregnancy.</p><p><strong>Conclusions: </strong>This study found that endometrial preparation protocols and embryonic development stages are significantly associated with ectopic pregnancy.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143709407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}