Journal of Assisted Reproduction and Genetics最新文献

{"title":"Exposure to nanoscale graphene oxide deteriorates the quality of porcine oocytes via induction of oxidative stress and the apoptosis.","authors":"Yang Gao, Fuziaton Baharudin, Yunhai Zhang, Kaixiang Tan, Yongteng Zhang, Mengchan Li, Zizheng Liang, Mengting Wu, Mianqun Zhang, Dandan Zhang","doi":"10.1007/s10815-025-03553-y","DOIUrl":"https://doi.org/10.1007/s10815-025-03553-y","url":null,"abstract":"<p><strong>Purpose: </strong>Nano graphene oxide (nGO), as a type of engineered carbon nanomaterial, has witnessed significant growth in biomedical applications. Given the likelihood of accumulation of these materials in human tissues or organs, it becomes imperative to comprehensively assess the toxicological profile of nGO, particularly concerning female reproductive health.</p><p><strong>Methods: </strong>Germinal vesicle (GV) porcine oocytes were cultured at 38.5 °C to the specific developmental stage for subsequent analysis. The nGO was diluted with the maturation medium to the final concentrations of 10, 50, 100 and 200 μg/ml, respectively. Immunostaining and fluorescence intensity quantification were applied to assess the effects of nGO exposure on the key processes during the oocyte meiotic maturation.</p><p><strong>Results: </strong>We observed that exposure to nGO led to compromised meiotic competency in porcine oocytes during in vitro culture. Specifically, nGO exposure resulted in reduced acetylation levels of α-tubulin and misattachment of kinetochore-microtubules, thereby disrupting spindle/chromosome organization and impeding meiotic progression. Furthermore, nGO exposure perturbed actin dynamics, potentially hindering spindle migration and cortical polarization during oocyte meiosis. Additionally, mislocalization and premature exocytosis of ovastacin were observed following nGO exposure. Notably, nGO exposure induced mitochondrial dysfunction, DNA damage, and oxidative stress, ultimately triggering apoptosis and impeding the maturation of porcine oocytes and the development of post-fertilized embryos.</p><p><strong>Conclusion: </strong>Our findings underscore the potential deleterious effects of nGO on mammalian oocyte quality, while also contributing valuable insights into the impact of environmental nanoparticle release on female germ cell development.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144540357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Autophagy-related biomarkers in non-obstructive azoospermia: insights from transcriptomics and single-cell sequencing.","authors":"Juncheng Yao, Yuanyuan Zhang, Tingting Lan, Yutao Li","doi":"10.1007/s10815-025-03559-6","DOIUrl":"https://doi.org/10.1007/s10815-025-03559-6","url":null,"abstract":"<p><strong>Objective: </strong>Autophagy, by modulating cellular degradation and recycling processes, affects sperm cell survival and differentiation and may be linked to the pathophysiology of non-obstructive azoospermia (NOA). However, the role of autophagy-related genes (ARGs) in NOA has not yet been fully explored.</p><p><strong>Methods: </strong>Transcriptomic datasets for NOA from public databases were used. By combining differential expression analysis between NOA and obstructive azoospermia (OA), weighted gene co-expression network analysis (WGCNA), and machine learning. algorithms, biomarkers were identified. Their performance was evaluated with ROC curves. Enrichment and immune analyses explored mechanisms, and validation was done via datasets (GSE9210 and GSE145467) and RT-qPCR. Single-cell level mechanisms were also studied.</p><p><strong>Results: </strong>A total of 321 differentially expressed genes (DEGs) were screened, related to germ cell development and nucleocytoplasmic transport. Four biomarkers (ATP6V1E2, UBQLN2, FYCO1, and ITPR1) from machine learning had good diagnostic performance. FYCO1 positively correlated with T follicular helper cells and negatively with regulatory T cells. Single-gene GSEA showed it was enriched in gametogenesis and cell adhesion molecules. Single-cell analysis revealed ATP6V1E2 had the highest expression in testicular gamete cells, while UBQLIN2, FYCO1, and ITPR1 in smooth muscle cells RT-qPCR results matched the dataset trends.</p><p><strong>Conclusion: </strong>This study screened four ARGs (ATP6V1E2, UBQLN2, FYCO1, and ITPR1) as biomarkers for NOA, which could serve as potential diagnostic tools and therapeutic targets, potentially providing new treatment strategies.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Successful pre-implantation genetic testing for autosomal recessive cutis laxa: clinical utility of a multidisciplinary team approach.","authors":"Qiuxiang Huang, Xiurong Yu, Lihua Mao, Chunli Lin, Caixia Wang, Juan Lin, Yun Liu, Zhihong Wang","doi":"10.1007/s10815-025-03565-8","DOIUrl":"https://doi.org/10.1007/s10815-025-03565-8","url":null,"abstract":"<p><strong>Purpose: </strong>Pre-implantation genetic testing for monogenic disorders (PGT-M) enables couples at risk of transmitting serious genetic disorders to their offspring to give birth to healthy children. Here, we present an illustrative case of the use of PGT-M in a couple who were both carriers of autosomal recessive cutis laxa (ARCL) and describe the application of PGT-M in our reproductive center using a multidisciplinary team approach.</p><p><strong>Methods: </strong>The couple experienced four adverse pregnancy outcomes, and both partners were subsequently identified as carriers of ARCL caused by PYCR1 gene variants during a genetic evaluation. They chose PGT-M after counseling and being fully informed of the potential risks and benefits. Trophectoderm cells were biopsied on day 5 or 6 and whole-genome amplification was performed using multiple annealing and looping-based amplification cycles (MALBAC). Mutated allele revealed by sequencing with aneuploidy and linkage analysis (MARSALA) was used to detect the copy number variations and the carrier status of the PYCR1 gene. Prenatal diagnosis was performed to validate the PGT-M results. Psychological support was provided throughout.</p><p><strong>Results: </strong>PGT-M was successfully performed, and transfer of a euploid blastocyst carrying a paternal variant in the third cycle resulted in a pregnancy. Prenatal diagnosis confirmed the PGT-M results. The woman gave birth to a healthy boy with normal skin and growth and developmental milestones.</p><p><strong>Conclusion: </strong>This study illustrates the clinical applicability of PGT-M in ARCL carrier couples, highlighting the importance of multidisciplinary consultation, and the implementation of comprehensive genetic counseling protocols and tailored psychosocial interventions.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sperm abnormality: Differential expression of microRNAs.","authors":"Sara Rahbar, Behnaz Sadeghzadeh Oskouei, Nahid Azad, Jafar Rezaie, Rana Jahanban Esfahlan","doi":"10.1007/s10815-025-03562-x","DOIUrl":"https://doi.org/10.1007/s10815-025-03562-x","url":null,"abstract":"<p><p>Adult male fertility requires continuous production of sperm through the spermatogenesis process involving the correct timing of regulatory signals to spermatogonial stem cells (SSCs) to support continuous gamete generation. MicroRNAs (miRNAs) modulate gene expression post-transcriptionally and are shown to regulate various biological pathways in both humans and animal models. miRNAs in Sertoli cells and germ cells respond to regulatory signals and downregulate or upregulate required mRNAs and proteins that function in different pathways to support successful spermatogenesis. In addition, differential expression of miRNAs in sperm and seminal plasma of men with sperm disorders and normozoospermia is reported. However, the role of miRNAs in the pathogenesis of patients with sperm disorders and its mechanisms have not been comprehensively studied. The aim of this review is to describe the mechanisms through which the dysregulation of miRNAs can lead to male infertility. Further, the possibility of using miRNAs as diagnostic and therapeutic targets is discussed.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"\"Forever fertile\": ovarian tissue cryopreservation for an extended reproductive lifespan.","authors":"Claudia Massarotti, Chiara Selmi, Antonio La Marca","doi":"10.1007/s10815-025-03554-x","DOIUrl":"https://doi.org/10.1007/s10815-025-03554-x","url":null,"abstract":"<p><p>The global decline in birth rates highlights the need for effective fertility preservation strategies. Even though oocyte cryopreservation is a well-established technique in cancer patients and is increasingly requested for elective fertility preservation, its success is limited by age at freezing and restoration of ovarian activity is not provided. Ovarian tissue cryopreservation is emerging as a promising alternative for both fertility preservation and reproductive lifespan extension. Unlike oocyte cryopreservation, ovarian tissue cryopreservation restores endocrine function, potentially delaying menopause and reducing associated health risks. Although concerns exist regarding graft longevity and surgical invasiveness, recent advancements-such as improved cryopreservation techniques, neovascularization strategies, and minimally invasive approaches-enhance its feasibility. Additionally, ovarian tissue cryopreservation allows for spontaneous conception, reducing the need for assisted reproductive technologies. As demand for reproductive longevity increases, the medical community must address ethical and regulatory implications while refining clinical applications. Integrating ovarian tissue cryopreservation into elective fertility preservation can provide women with more reproductive choices, aligning with advances in longevity medicine. Future research should focus on optimizing graft survival and assessing long-term health outcomes of delayed menopause to fully unlock the potential of ovarian tissue cryopreservation.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Developing a live birth prediction model for single euploid frozen embryo transfer using machine learning: a novel approach.","authors":"Tiantaixi Tu","doi":"10.1007/s10815-025-03568-5","DOIUrl":"https://doi.org/10.1007/s10815-025-03568-5","url":null,"abstract":"","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prospective evaluation of mid-luteal endometrial BCL6/SIRT and correlation with outcomes of euploid frozen embryo transfer: a prospective cohort study.","authors":"Michael Strug, Lusine Aghajanova, Maliha Khan, Jiaqi Zhang, Dan Angress, Steven L Young, Bruce Lessey, Ruth B Lathi","doi":"10.1007/s10815-025-03558-7","DOIUrl":"https://doi.org/10.1007/s10815-025-03558-7","url":null,"abstract":"<p><strong>Purpose: </strong>To study whether mid-luteal endometrial B-cell lymphoma 6 (BCL6) or sirtuin-1 (SIRT1) immunostaining in an ovarian stimulation (fresh) in vitro fertilization (IVF) cycle was predictive of risk for endometriosis. Additionally, to evaluate for association with future euploid frozen embryo transfer (FET) pregnancy outcomes.</p><p><strong>Methods: </strong>Prospective, blinded observational cohort study in an academic fertility center. Patients pursuing IVF with euploid FET within 1 year who met inclusion criteria for one of three groups: (G1) surgically confirmed endometriosis (n = 10), (G2) unexplained infertility or recurrent pregnancy loss (n = 42), or (G3) controls without identifiable female infertility (n = 24). BCL6 and SIRT1 immunostaining was quantified in endometrial samples obtained 5-7 days after oocyte retrieval (HSCORE > 1.4 considered positive). Euploid FET in a subsequent cycle was blinded to BCL6/SIRT1 assessment. Demographic and pregnancy outcomes for each group were correlated with BCL6/SIRT1 levels.</p><p><strong>Results: </strong>There was high BCL6 positivity with a significant interaction among groups (G1: 80%, G2: 97.6%, G3: 100%; p = 0.044), but pairwise comparisons did not demonstrate a difference between individual groups. Median BCL6 levels by H-SCORE were similarly high in all groups [median (inter-quartile range); G1: 3.7 (3.45, 4.0), G2: 3.8 (3.6, 3.8), G3: 3.8 (3.4, 4.0); p = 0.95]. Median SIRT1 levels also did not differ between groups. Pregnancy outcomes following FET were comparable between groups (live birth rate G1: 57.1%, G2: 62.1%, G3: 54.5%; p = 0.68). BCL6 levels were inversely correlated with serum progesterone level on the day of endometrial biopsy (τ =  - 0.223, p = 0.01). Correlation analysis of pregnancy outcomes for all patients included in the study revealed no difference between BCL6 or SIRT1 levels for patients who did or did not experience a live birth.</p><p><strong>Conclusion: </strong>Endometrial BCL6 and SIRT1 levels collected in a fresh ovarian stimulation cycle did not correlate with endometriosis diagnosis nor pregnancy outcomes. BCL6 levels were inversely correlated with serum progesterone levels.</p><p><strong>Clinical trial id: </strong>NCT0410712.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144505793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sirtuins, redox, and metabolic pathways in the brain of female PCOS mice.","authors":"Teresa Vergara, Giovanni Casoli, Andrea Bianchi, Martina Placidi, Maria Grazia Palmerini, Domenica Cocciolone, Stefano Falone, Arturo Bevilacqua, Carla Tatone, Valeria Cordone, Giovanna Di Emidio","doi":"10.1007/s10815-025-03557-8","DOIUrl":"https://doi.org/10.1007/s10815-025-03557-8","url":null,"abstract":"<p><strong>Purpose: </strong>Recent studies emphasize the role of neuroendocrine dysfunctions and sirtuins in polycystic ovarian syndrome (PCOS). We investigated whether altered SIRT1 and SIRT3 levels contribute to brain changes and oxidative stress, identifying these pathways as potential therapeutic targets for PCOS-related complications.</p><p><strong>Methods: </strong>Using a DHEA-induced PCOS mouse model, we examined brain expression of pathways related to SIRT1 and SIRT3 and to oxidative/glycative stress changes. SH-SY5Y cells treated with DHEA were used to confirm direct neuronal effects.</p><p><strong>Results: </strong>We found decreased levels of Sirt1 and Sirt3 transcripts but increased protein expression and activity of both sirtuins in brains of DHEA-treated mice. The DHEA group showed elevated oxidative and glycative stress, including an overall increased lipid peroxidation and DNA damage, as well as accumulation of advanced glycation endproducts (AGEs) in isocortices. Differences in Cpt1 isoform expressions suggested disrupted metabolic processing in the PCOS brains. Neuronal degeneration was also observed, alongside unchanged Bdnf and TrkB mRNA levels in DHEA brains. Exposure of differentiated SH-SY5Y neuron-like cells to high concentrations (≥ 100 µM) led to increased oxidative stress, altered sirtuins expression, and ultimately cell toxicity. While low concentrations of DHEA (1 µM) did not elicit such responses.</p><p><strong>Conclusions: </strong>These findings reveal a complex interplay between oxidative stress, metabolic dysregulation, and neuronal health in PCOS brain, underscoring the need for further investigations into the underlying mechanisms, including research in genetic components. This research provides foundational insights into how PCOS may influence neurobiological processes and helps clarify some aspects of its pathogenesis.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144496787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Three different protocols for pituitary suppression: progestins (dydrogesterone or medroxyprogesterone acetate) versus GnRH antagonist-balancing efficacy and cost in ovarian stimulation.","authors":"Pınar Özcan, Emre Goksan Pabuccu, Ege Mertoğlu, Tunç Timur, Elif Cigdem Keleş, Bilge Pınar Keskinsoy, Recai Pabuççu","doi":"10.1007/s10815-025-03555-w","DOIUrl":"https://doi.org/10.1007/s10815-025-03555-w","url":null,"abstract":"<p><strong>Purpose: </strong>To compare the clinical effectiveness and overall treatment cost of three ovarian stimulation protocols-dydrogesterone (DYD), medroxyprogesterone acetate (MPA), and GnRH antagonist-in women undergoing in vitro fertilization (IVF).</p><p><strong>Methods: </strong>This prospective, multicenter cohort study was conducted at two IVF units from March 2023 to March 2024. A total of 307 women undergoing IVF were divided into three groups based on their pituitary suppression protocol: DYD (n = 99), MPA (n = 101), and GnRH antagonist (n = 107). Ovarian stimulation parameters, pregnancy outcomes, and detailed cost analyses were then compared across these groups.</p><p><strong>Results: </strong>The number of mature oocytes (MII) retrieved and follicular output rate (FOI) were comparable among the three groups (MII, p = 0.67; FOI, p = 0.74). Gonadotropin consumption and estradiol levels on trigger day were significantly higher in the MPA group (p < 0.001 and p = 0.009, respectively). Clinical pregnancy rates (DYD 37.4%, MPA 32.7%, GnRH antagonist 34.6%; p = 0.78) and ongoing pregnancy rates (DYD 32.3%, MPA 28.7%, GnRH antagonist 29.9%; p = 0.85) did not differ significantly among groups. While the LH suppression cost per cycle was highest in the GnRH antagonist group (257.7 USD), the total cycle cost for this group was the lowest, as it typically involves fresh embryo transfer compared to frozen embryo transfer (FET) in PPOS (progestin-primed ovarian stimulation) protocols.</p><p><strong>Conclusion: </strong>PPOS protocols (DYD or MPA) offer clinical outcomes comparable to the GnRH antagonist protocol. While PPOS regimens may provide cost advantages in freeze-all settings due to lower LH suppression, the overall economic benefit hinges on the embryo transfer strategy. Therefore, optimal protocol selection should be individualized, considering both clinical characteristics and cost.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144496788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seminal hyperviscosity is associated with poor sperm quality and function.","authors":"Jiao Qin, Qingyuan Cheng, Yingbi Wu, Siyu Long, Yuanyuan Zeng, Wenrui Zhao, Lin Yu, Fuping Li","doi":"10.1007/s10815-025-03551-0","DOIUrl":"https://doi.org/10.1007/s10815-025-03551-0","url":null,"abstract":"<p><strong>Purpose: </strong>Male infertility affects many couples worldwide. Seminal hyperviscosity (SHV) is increasingly recognized as a contributor to male infertility by impairing sperm movement. However, the precise mechanisms by which SHV affects sperm parameters and functions remain unclear.</p><p><strong>Methods: </strong>This is a retrospective study. We evaluated the prevalence of subjects with SHV in a large population (including 55,733 semen samples) referring to the Department of Andrology, West China Second University Hospital, as well as the relationship between SHV and sperm quality, DNA damage, Acrosome function, mitochondria function, and seminal plasma composition.</p><p><strong>Results: </strong>SHV was identified in 12.4% of samples (mild: 7.7%, moderate: 4.1%, severe: 0.6%), with severity correlating to poorer semen parameters, including reduced volume, total sperm count, progressive motility (PR), viability, and normal morphology (all p < 0.05). Notably, SHV samples exhibited impaired acrosomal function and lower mitochondrial membrane potential (all p < 0.05), despite paradoxically lower DNA fragmentation indices (DFI) in mild/moderate cases. Severe SHV showed elevated high DNA stainability (HDS) and chromatin condensation anomalies (p < 0.05). Seminal plasma analysis revealed higher rates of abnormal fructose levels in SHV groups, suggesting seminal vesicle dysfunction as a potential etiology.</p><p><strong>Conclusion: </strong>These findings highlight SHV's multifaceted detrimental effects on sperm functionality beyond conventional motility metrics, emphasizing its clinical relevance in fertility assessments. All these results remind that clinicians should pay more attention to the index of semen viscosity. In addition, standardized viscosity evaluation protocols and targeted interventions for SHV-related glandular deficiencies are warranted to improve diagnostic accuracy and treatment outcomes.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144484536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}