Journal of Assisted Reproduction and Genetics最新文献

Beyond the scale: navigating BMI, IVF candidacy, and retrieval settings through provider perspectives. 超越尺度：导航BMI，试管婴儿候选资格，并通过提供者的观点检索设置。

IF 2.7 3区医学

Journal of Assisted Reproduction and Genetics Pub Date : 2025-08-01 DOI: 10.1007/s10815-025-03604-4

Amanda Tjitro, Lisa Pappas, Erica Boiman Johnstone, Megan Link, Lauren Verrilli, Meredith Humphreys, Ijeoma Iko, Joseph Letourneau

{"title":"Beyond the scale: navigating BMI, IVF candidacy, and retrieval settings through provider perspectives.","authors":"Amanda Tjitro, Lisa Pappas, Erica Boiman Johnstone, Megan Link, Lauren Verrilli, Meredith Humphreys, Ijeoma Iko, Joseph Letourneau","doi":"10.1007/s10815-025-03604-4","DOIUrl":"https://doi.org/10.1007/s10815-025-03604-4","url":null,"abstract":"Purpose: To identify provider-level differences in the role of body mass index (BMI) in in vitro fertilization (IVF).Methods: A cross-sectional survey was distributed electronically to Society of Reproductive Endocrinology and Infertility members (n = 827). Analysis implemented descriptive statistics and Fisher's exact tests.Results: One hundred eighty-seven responses were received from practices across the USA (22% response rate). Eighty-four percent of responders turned away at least one patient, in the past year, due to either BMI (82%) or a medical comorbidity other than obesity (68%). Eighty-six percent of respondents implement a cutoff. Implementation of cutoffs did not differ significantly by practice setting or geographic region. The most common reported BMI cutoff was ≥ 40. 95% cited anesthesia requirements as the primary reason for cutoffs. Only 11% of respondents perform hospital retrievals, where all 20 providers were academically affiliated. Those not performing retrievals cited lack of access to a mobile embryology lab (60%), patient cost (39%), and scheduling difficulties (55%) as common barriers.Conclusions: Most surveyed REIs said they had excluded a patient due to BMI or a medical comorbidity in the past year, likely due to surgical and anesthetic concerns. Very few providers have access to hospital retrievals, but even outpatient retrievals for women with BMI ≥ 40 are considered safe. We agree with avoidance of a universal BMI cutoff; rather, we advocate for streamlined referral systems for otherwise excluded women, and further work in weight loss management interventions and cost effectiveness of IVF.","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144760221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microplastic exposure induces epithelial barrier alterations and increases collagen deposition in a 3D human endometrial model in vitro. 微塑料暴露诱导上皮屏障改变并增加体外3D人子宫内膜模型中的胶原沉积。

IF 2.7 3区医学

Journal of Assisted Reproduction and Genetics Pub Date : 2025-08-01 DOI: 10.1007/s10815-025-03566-7

Sharon Arcuri, Georgia Pennarossa, Daniela Bebbere, Fulvio Gandolfi, Sergio Ledda, Tiziana A L Brevini

{"title":"Microplastic exposure induces epithelial barrier alterations and increases collagen deposition in a 3D human endometrial model in vitro.","authors":"Sharon Arcuri, Georgia Pennarossa, Daniela Bebbere, Fulvio Gandolfi, Sergio Ledda, Tiziana A L Brevini","doi":"10.1007/s10815-025-03566-7","DOIUrl":"https://doi.org/10.1007/s10815-025-03566-7","url":null,"abstract":"Purpose: To investigate the effects of microplastics (MPs) on the human endometrium in vitro.Methods: A predictive 3D endometrial in vitro model was generated using highly porous scaffolds where human endometrial stromal (hESC) and epithelial (hEEC) cells were co-cultured for 35 days. The newly generated endometrial barrier was then exposed to different MP concentrations (from 0.25 to 50 mg/ml) for 24 h and 48 h, respectively. Histological staining and functional analyses were performed to assess the endometrial barrier integrity. Molecular studies and collagen deposition were evaluated to investigate the possible activation of pro-apoptotic and pro-fibrotic related pathways.Results: MP exposure for 24 h does not affect endometrial barrier integrity nor collagen synthesis and deposition. Similar responses are detected when concentrations between 0.25 and 1 mg/ml are used for 48 h. In contrast, 48-h incubations with higher doses (10-50 mg/ml MPs) induce epithelial barrier alterations, reduce TEER values and decrease ZO1 and CDH1 gene transcription. This is accompanied by the activation of pro-fibrotic signalling pathways resulting in collagen increment, which often accompanies endometriosis-related alterations.Conclusion: The data obtained suggest MP ability to exert deleterious effects in vitro on human endometrium, with a possible negative impact on its functionality and receptivity.","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144760222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-viscosity oil does not offer enhanced protection for osmolality changes in high-volume media drops during extended dry incubation. 在长时间的干燥培养过程中，高粘度油不能为大容量介质滴的渗透压变化提供增强的保护。

IF 2.7 3区医学

Journal of Assisted Reproduction and Genetics Pub Date : 2025-07-29 DOI: 10.1007/s10815-025-03586-3

Louise Murray, Nairuti Patel, Sinan Ozkavukcu, Federica Lopes

{"title":"High-viscosity oil does not offer enhanced protection for osmolality changes in high-volume media drops during extended dry incubation.","authors":"Louise Murray, Nairuti Patel, Sinan Ozkavukcu, Federica Lopes","doi":"10.1007/s10815-025-03586-3","DOIUrl":"https://doi.org/10.1007/s10815-025-03586-3","url":null,"abstract":"Purpose: While new technologies support undisturbed embryo culture, non-humidified incubation raises concerns about medium stability. High-viscosity oil (HVO) was proposed to maintain osmolality, but its validation remains limited. This study investigates whether HVO offers advantages over conventional light oil (CLO) in preventing evaporation during extended dry incubation using a high-volume media drop setting and its impact on embryo development and temperature control.Methods: HVO and CLO were overlaid on embryo culture media drops and incubated for 5 days under either dry or humid conditions. At various intervals throughout the experiment, measurements of osmolality, pH, temperature, and sodium concentration were taken. To assess toxicity, mouse embryo assays and lipid peroxidation detection were conducted in post-culture media.Results: The dry incubation significantly increased osmolality and sodium concentrations, regardless of oil type, from the first day of incubation, under the current experimental conditions. After 5 days of incubation, HVO was not superior to CLO in preventing these changes under dry conditions (289.867 ± 1.10 vs. 284.93 ± 1.03, respectively, p > 0.05). The mean osmolality values in CLO vs. HVO in dry conditions provided mild protection against temperature loss when dishes were removed from incubators and exposed to room temperature for 5 min, simulating a suboptimal setting. No significant differences were observed between HVO and CLO regarding lipid peroxidation or embryo development.Conclusion: These findings indicate that a high-viscosity oil does not confer better protection against evaporation than a conventional oil when uninterrupted culture is performed in dry incubation and high-volume media drops are used.","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144731150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Triplet pregnancies after single blastocyst embryo transfer-how often and why do these occur? 单囊胚移植后三胞胎妊娠——发生的频率和原因？

IF 2.7 3区医学

Journal of Assisted Reproduction and Genetics Pub Date : 2025-07-28 DOI: 10.1007/s10815-025-03593-4

Ariel Woods, Steven R Lindheim, Saira Kothari, Dean E Morbeck, Emily N Liu, Amy Zhu, Nigel Pereira

{"title":"Triplet pregnancies after single blastocyst embryo transfer-how often and why do these occur?","authors":"Ariel Woods, Steven R Lindheim, Saira Kothari, Dean E Morbeck, Emily N Liu, Amy Zhu, Nigel Pereira","doi":"10.1007/s10815-025-03593-4","DOIUrl":"https://doi.org/10.1007/s10815-025-03593-4","url":null,"abstract":"Single embryo transfer (sET) has been adopted by assisted reproductive technology (ART) clinics globally to increase the rate of singleton births and reduce multi-fetal pregnancies. Independent ART registry-based studies have reported an association between monozygotic (MZ) splitting and sET, especially after single blastocyst embryo transfer (sBET). While MZ twins after sBET are not uncommon, MZ triplet pregnancies after sBET are exceptionally rare. In this context, we report two cases of MZ triplet pregnancies after sBET. The first case is of a 29-year-old woman with a unicornuate uterus who conceived a tri-chorionic triplet pregnancy after single euploid blastocyst transfer in a medicated frozen embryo transfer (FET) cycle. The second case is of a 34-year-old woman with a triplet pregnancy consisting of monochorionic diamniotic twins and a singleton after the transfer of an untested blastocyst in a natural FET cycle. Using these cases, we focus on the epidemiology and putative mechanisms of MZ splitting. Given that sBET does not eliminate the risk of multiple pregnancy entirely, it is important to counsel couples about the risks of MZ twins and triplets with sBET.","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144731152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Does ovarian morphology type affect the reproductive outcomes of in-vitro maturation cycles in polycystic ovary syndrome? 卵巢形态类型是否影响多囊卵巢综合征体外成熟周期的生殖结果？

IF 2.7 3区医学

Journal of Assisted Reproduction and Genetics Pub Date : 2025-07-28 DOI: 10.1007/s10815-025-03610-6

Alyssa Hochberg, Shai Elizur, Bernice Goorah, Samer Tannus, Weon Young Son, Michael H Dahan

{"title":"Does ovarian morphology type affect the reproductive outcomes of in-vitro maturation cycles in polycystic ovary syndrome?","authors":"Alyssa Hochberg, Shai Elizur, Bernice Goorah, Samer Tannus, Weon Young Son, Michael H Dahan","doi":"10.1007/s10815-025-03610-6","DOIUrl":"https://doi.org/10.1007/s10815-025-03610-6","url":null,"abstract":"Purpose: To examine whether ovarian morphology in polycystic ovary syndrome (PCOS) affects reproductive outcomes in in-vitro-maturation (IVM) cycles.Methods: A two-year, single-center retrospective cohort study including PCOS patients undergoing IVM. PCOS diagnosis followed the 2003 Rotterdam criteria, and all subjects had a sonographic criterion (≥ 12 follicles in one ovary). Patients were categorized based on ovarian morphology imaged by trans-vaginal ultrasonography: i)peripheral cystic pattern (PCP), with follicles at the ovarian periphery; ii)general cystic pattern (GCP), with follicles dispersed throughout the ovarian stroma.Results: Of 127 subjects, 72 were in the PCP group and 55 in the GCP group. Women with PCP, compared to GCP, had higher rates of hirsutism and oligomenorrhea, and a higher antral follicle count. IVM outcomes showed that women with PCP had significantly more total retrieved oocytes; additional metaphase II oocytes 24- and 48-h post-retrieval;total metaphase II oocytes; IVM metaphase II oocytes; total cleavage stage embryos; and total good-quality embryos. PRs and CPRs were comparable between groups. Multivariable logistic regression adjusting for ovarian morphology type, female age,male age, gravidity, and total testosterone levels, demonstrated that ovarian morphology was not associated with PR or CPR. However, PCP was a predictor for ≥ 10 retrieved oocytes (aOR 12.5, 95%CI 2.44-100,p = 0.003),and ≥ 15 retrieved oocytes (aOR 10, 95%CI 1.75-50,p = 0.009). Additionally, increasing gravidity was associated with a reduced likelihood of ≥ 10 retrieved oocytes (aOR 0.48, 95%CI 0.26-0.91,p = 0.024).Conclusions: PCP ovarian morphology in PCOS patients correlated with higher oocyte and mature oocyte yield, cleavage stage embryos,and good-quality cleavage stage embryos, while pregnancy outcomes were comparable.","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144731138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oocyte cryopreservation for fertility preservation in transgender and gender diverse individuals: a SWOT analysis. 跨性别和性别差异个体的卵母细胞冷冻保存保存生育能力：SWOT分析。

IF 2.7 3区医学

Journal of Assisted Reproduction and Genetics Pub Date : 2025-07-28 DOI: 10.1007/s10815-025-03579-2

Julia E Barbe, Federico Del Vento, Dehlia Moussaoui, Victoria L Crofts, Michal Yaron, Isabelle Streuli

引用次数: 0

An efficient advanced YOLOv8 framework for sperm motility detection. 高效先进的YOLOv8精子活力检测框架。

IF 2.7 3区医学

Journal of Assisted Reproduction and Genetics Pub Date : 2025-07-28 DOI: 10.1007/s10815-025-03589-0

Chuanjiang Li, Wenjing Xia, Ang Li, Liangshou Gao, Chongming Zhang, Erlei Zhi, Zheng Li

{"title":"An efficient advanced YOLOv8 framework for sperm motility detection.","authors":"Chuanjiang Li, Wenjing Xia, Ang Li, Liangshou Gao, Chongming Zhang, Erlei Zhi, Zheng Li","doi":"10.1007/s10815-025-03589-0","DOIUrl":"https://doi.org/10.1007/s10815-025-03589-0","url":null,"abstract":"Purpose: Sperm motility detection is crucial for reproductive health research, which is closely related to human development. Due to the small size and fast movement of sperm, coupled with the complex background, traditional image processing methods can hardly obtain accurate detection results.Methods: To address these issues, an advanced YOLOv8-based sperm detection model is proposed, named YOLOv8-STA. First, the SPD_Conv structure is integrated into the backbone network to improve the ability for extracting fine-grained information about sperm targets. Secondly, in order to construct effective relationships between input features while preserving more original image information, the partial C2F modules in the backbone and neck networks of the YOLOv8 model are replaced with C2F-Triplet modules, which can capture interactions across dimensions, build channel, and spatial attentions with minimal parameters. Finally, a new 104 <math><mo>×</mo></math> 104 pixel small object detection head is added specifically in the detection layer to construct advanced feature maps.Results: Experimental results on the public EVISAN dataset show that the improved YOLOv8-STA model can increase the mAP@0.5 by 4.2% and mAP@0.5:0.95 by 5.4%, compared with the baseline YOLOv8 model.Conclusion: These experimental results verify the effectiveness and superiority of the proposed method in sperm motility detection.","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144731137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigations on semen analysis capabilities and quality control measures in laboratories with different management modes. 不同管理模式实验室精液分析能力及质量控制措施的调查。

IF 3.2 3区医学

Journal of Assisted Reproduction and Genetics Pub Date : 2025-07-26 DOI: 10.1007/s10815-025-03595-2

Hong-Lin Li, Jin-Chun Lu, Chao Zhao, Xi Zhang

{"title":"Investigations on semen analysis capabilities and quality control measures in laboratories with different management modes.","authors":"Hong-Lin Li, Jin-Chun Lu, Chao Zhao, Xi Zhang","doi":"10.1007/s10815-025-03595-2","DOIUrl":"https://doi.org/10.1007/s10815-025-03595-2","url":null,"abstract":"Purpose: To perform a survey on the semen analysis capabilities and quality control measures of medical institutions conducting male infertility-related testing in Jiangsu, China, by the WeChat, aiming to explore the possibility of mutual recognition of testing results among different laboratories.Methods: This study designed a survey questionnaire consisting of 105 questions and investigated and compared semen analysis capabilities and quality control measures between regulated laboratories for assisted reproductive technology (RLART) and non-regulated laboratories for assisted reproductive technology (non-RLART) in Jiangsu, China.Results: RLART had higher percentages of technicians received training on the WHO manual (97.14% [34/35] vs. 57.14% [28/49], P < 0.01), dedicated rooms for semen collection (100% [35/35] vs. 24.49% [12/49], P < 0.01), adherence to the staining methods recommended by the WHO manual (100% [32/32] vs. 33.33% [11/33], P < 0.01), internal quality control for sperm concentration (77.14% [27/35] vs. 6.12% [3/49], P < 0.01), and participation in external quality assessment (74.29% [26/35] vs. 14.29% [7/49], P < 0.01) compared to non-PLART.Conclusions: Establishing unified recognition criteria for semen analysis laboratories and conducting regular supervision can improve semen analysis capabilities and promote mutual recognition of semen testing results.","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144717983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sperm DNA fragmentation index did not associate with assisted reproductive outcomes: a cohort retrospective study of 5784 IVF/ICSI cycles. 精子DNA断裂指数与辅助生殖结果无关：一项5784个IVF/ICSI周期的队列回顾性研究。

IF 3.2 3区医学

Journal of Assisted Reproduction and Genetics Pub Date : 2025-07-26 DOI: 10.1007/s10815-025-03574-7

Ting-Feng Fang, Yan-Hong Zhang, Wen-Chang Yu, Neng-Yong Ouyang, Jing-Hua Chen, Hai-Xia Liang, Hui-Ling Zhong, Hui Chen, Yiu-Leung Chan, Wen-Jun Wang

{"title":"Sperm DNA fragmentation index did not associate with assisted reproductive outcomes: a cohort retrospective study of 5784 IVF/ICSI cycles.","authors":"Ting-Feng Fang, Yan-Hong Zhang, Wen-Chang Yu, Neng-Yong Ouyang, Jing-Hua Chen, Hai-Xia Liang, Hui-Ling Zhong, Hui Chen, Yiu-Leung Chan, Wen-Jun Wang","doi":"10.1007/s10815-025-03574-7","DOIUrl":"https://doi.org/10.1007/s10815-025-03574-7","url":null,"abstract":"Purpose: The debate regarding the predictive value of the sperm DNA fragmentation index (DFI) on assisted reproductive technology (ART) outcomes continues. This study aimed to investigate the impact of sperm DFI on IVF/ICSI outcomes.Methods: This retrospective cohort study included 4319 patients undergoing 5784 fresh IVF/ICSI cycles from January 2015 to December 2022. Data on clinical characteristics, embryological development, pregnancy, and neonatal outcomes were collected to assess the differences between the DFI ≤ 30% and > 30% groups in IVF/ICSI cycles.Results: The mean (SD) sperm DFI were 10.85 (6.36) % for IVF cycles and 12.96 (7.24) % for ICSI cycles in the DFI ≤ 30% group. Conversely, the mean (SD) DFI were 39.53 (8.83) % for IVF cycles and 42.58 (11.58) % for ICSI cycles in the DFI > 30% group. No significant differences were observed between the DFI ≤ 30% and > 30% groups in 2PN fertilization rate, blastocyst formation rate, implantation rate, clinical pregnancy rate, live birth rate, and miscarriage rate (P > 0.05). A total of 1471 neonates were followed, and the incidence of congenital malformations was comparable across the two groups (P > 0.05). Multivariable analyses showed male age significantly associated with sperm DFI. Compared with men aged < 35 years, those aged 35-45 and > 45 years had higher DFI risk (adjusted odds ratio [OR] = 1.37, 95% CI: 1.06-1.77, P = 0.016; adjusted OR = 2.29, 95% CI: 1.05-5.02, P = 0.038). Immotile sperm percentage also positively correlated with DFI (adjusted OR = 1.04, 95% CI: 1.03-1.05, P < 0.001).Conclusion: Elevated sperm DFI did not significantly impact IVF/ICSI outcomes, suggesting limited utility as a routine pretreatment assessment in ART.","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144717984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cellular therapies in primary ovarian insufficiency and poor ovarian reserve: a systematic review. 细胞治疗原发性卵巢功能不全和卵巢储备不良：系统综述。

IF 3.2 3区医学

Journal of Assisted Reproduction and Genetics Pub Date : 2025-07-24 DOI: 10.1007/s10815-025-03599-y

Burakhan Seyihoglu, Sude Gucuk, Alp Can

{"title":"Cellular therapies in primary ovarian insufficiency and poor ovarian reserve: a systematic review.","authors":"Burakhan Seyihoglu, Sude Gucuk, Alp Can","doi":"10.1007/s10815-025-03599-y","DOIUrl":"https://doi.org/10.1007/s10815-025-03599-y","url":null,"abstract":"Purpose: To evaluate the efficacy, safety, and limitations of cellular therapy in primary ovarian insufficiency (POI) and poor ovarian response (POR).Methods: A comprehensive search was conducted through the PubMed, Scopus, Web of Science, and Cochrane databases. The study type was limited to clinical trials, pilot studies, and case reports performed on human patients. Animal experiments, in vitro studies, correlational and longitudinal studies, and study designs and protocols were excluded. Twenty-seven studies with 694 patients from 2014 were found by May 2025.Results: Most studies (59.3%) were from Asia, and 77.8% lacked control groups. Autologous bone marrow mesenchymal stem/stromal cells (MSCs) were used in 37% of studies, rising to 51.8% when combined with platelet-rich plasma. Intraovarian injection was the most common delivery method (70.3%), administered laparoscopically (29.6%), or transvaginally (37%). Follow-up durations of 7-12 months were most common (40.7%). Pregnancy outcomes were reported in 59.2% of studies with control groups. Of the 522 patients treated, 60 (11.7%) became pregnant, compared to 13 (7.6%) of 172 controls. In two-arm studies (22.2% of all), the pregnancy rate was 22.6% (40 patients). Menstruation resumed in 37 (7.1%) of 522 treated patients, dropping to 5.1% in controlled studies.Conclusion: Variability in sample sizes and protocols affects the reliability of findings. Larger trials and standardized protocols for cell type, delivery, and outcomes are essential. Seven to twelve months of follow-up seems suitable for evaluating efficacy but may not be optimal for evaluating the safety. Stratifying patients by age, cause, and reserve could improve outcomes.Trial registration: Prospero (ID: CRD420251047104).","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144698632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0