Journal of Assisted Reproduction and Genetics最新文献

{"title":"Abnormal sperm tails correlate with the sperm DNA fragmentation index, irrespective of progressive motility, according to an analysis of 5125 semen reports.","authors":"Yanjing Wang, Hongping Li, Minhao Hu, Xiaozhen Liu, Lejun Li","doi":"10.1007/s10815-025-03452-2","DOIUrl":"https://doi.org/10.1007/s10815-025-03452-2","url":null,"abstract":"<p><strong>Purpose: </strong>Sperm progressive motility (PR) is correlated with the sperm DNA fragmentation index (SDF). Moreover, abnormalities in sperm tails are closely associated with the quality of sperm PR. However, the correlation of abnormal sperm tails with the SDF remains unknown.</p><p><strong>Methods: </strong>Routine semen examination was performed according to the World Health Organization guidelines with a computer-assisted semen analysis system. SDF analysis was conducted via the sperm chromatin structure assay method. Spearman correlation was used to test the correlation of the SDF with age and conventional semen parameters. The relationship between the SDF and abnormal tails was explored by binary logistic regression analysis, restricted cubic spline (RCS) analyses and subgroup analysis. ROC curves were used to assess the predictive capacity of age, abnormal tails and PR for the SDF.</p><p><strong>Results: </strong>The SDF was associated with sperm morphology, with a significant correlation observed with abnormal sperm tails (r = 0.491, P < 0.001). Binary regression analysis revealed that abnormal sperm tails were an independent influencing factor of the SDF. RCS analyses and Trend analysis suggested that as the number of abnormal sperm tails increased, the risk of an abnormal SDF also increased, and the trend was statistically significant (P for trend < 0.001). The ROC curve demonstrated that abnormal sperm tails had good discriminatory ability for the SDF (AUC: 0.757, P < 0.001).</p><p><strong>Conclusion: </strong>Abnormal sperm tails are an independent influencing factor of the SDF, and a higher number of abnormal tails is associated with an increased risk of an abnormal SDF.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143709967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of endometrial preparation protocols and embryonic development stages with ectopic pregnancy in freeze-thaw cycles.","authors":"Jinliang Zhu, Wan Yang, Ming Li, Xinjie Zhuang, Yapeng Wang, Ying Lian, Rong Li, Ping Liu","doi":"10.1007/s10815-025-03451-3","DOIUrl":"https://doi.org/10.1007/s10815-025-03451-3","url":null,"abstract":"<p><strong>Purpose: </strong>To investigate the correlation between endometrial preparation protocols, embryonic development stages, and ectopic pregnancy.</p><p><strong>Methods: </strong>A total of 29,700 clinical pregnancies, all of which resulted from freeze-thaw cycles performed between January 2018 and December 2023, were analyzed in this retrospective study.</p><p><strong>Results: </strong>The ectopic pregnancy rate was significantly higher in artificial cycles than natural cycles (1.5% vs. 0.8%; P < 0.001); the ectopic pregnancy rate was significantly higher in stage 5-6 than stage 3-4 blastocyst transfer (1.3% vs. 0.7%; P = 0.019). After incorporating all independent confounders into a multivariate general estimating equation model, artificial versus natural cycle (adjusted RR = 1.68, 95% CI = 1.32-2.13; P < 0.001) was shown to be an increased risk factor for ectopic pregnancy, especially in the single embryo transfer subgroup (aRR = 1.75, 95% CI = 1.23-2.48; P = 0.002); additionally, day 3 embryo transfer versus blastocyst transfer (aRR = 1.97, 95% CI = 1.15-3.38; P = 0.014) and stage 5-6 blastocyst versus stage 3-4 blastocyst transfer (aRR = 1.70, 95% CI = 1.05-2.74; P = 0.031) were shown to be increased risk factors for ectopic pregnancy.</p><p><strong>Conclusions: </strong>This study found that endometrial preparation protocols and embryonic development stages are significantly associated with ectopic pregnancy.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143709407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Personalized prediction model for miscarriage: in-depth sperm DNA fragmentation.","authors":"Chun-I Lee, Tse-En Wang, Chien-Hong Chen, Chun-Chia Huang, Hui-Mei Tsao, Tsung-Hsien Lee, Tiencheng Arthur Chang, Cheng-Teng Hsu, Maw-Sheng Lee","doi":"10.1007/s10815-025-03445-1","DOIUrl":"https://doi.org/10.1007/s10815-025-03445-1","url":null,"abstract":"<p><strong>Purpose: </strong>We investigated how different types of sperm DNA fragmentation (SDF) in males, in conjunction with varying levels of female reproductive potential, jointly impact ICSI outcomes.</p><p><strong>Methods: </strong>We retrospectively analyzed 195 couples undergoing ICSI, categorizing them by normal or poor prognosis according to POSEIDON criteria. Female factors included age, anti-Müllerian hormone (AMH), and oocyte retrieval numbers. Male factors included semen parameters, total SDF, and specific double-strand breaks (DSBs). Reproductive outcomes were followed up at different gestational stages, including clinical pregnancy, early gestation failure, live birth, and miscarriage. Risk factors were identified using univariate and multivariable logistic regression, and their predictive power was assessed via the receiver operating characteristic (ROC) curve.</p><p><strong>Results: </strong>In the normal group, female factors were primarily associated with reproductive failures. Non-pregnancy cases had lower AMH (4 ng/mL vs. 3.2 ng/mL), and miscarriage cases had fewer oocytes retrieved (15 vs. 10.5). However, the risk factor profile was distinct in poor prognosis. Male factors, including reduced sperm motility (68% vs. 54.5%), lower normal sperm morphology (5.5% vs. 2.5%), and elevated DSBs (7.5% vs. 18.8%) were linked to miscarriage. DSBs presented as the independent predictor of miscarriage risk (odds ratio: 1.19, 95% CI: 1.04-1.36), with a DSB cutoff of 19% providing 81% accuracy in predicting miscarriage.</p><p><strong>Conclusion: </strong>Paternal effect is pronounced in women with poor prognosis, where elevated DSBs are linked to an increased risk of miscarriage. We propose a refined pipeline in which DSB testing is considered as initial evaluation before assisted reproductive treatments, especially for infertile couples with poor prognosis.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143709417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Response to: Critical appraisal of \"pregnancy outcomes in patients with non-obstructive azoospermia undergoing micro-TESE: comparison of fresh vs. frozen-thawed testicular sperm\".","authors":"Lala Suleymanova, Hale Bayram, Yaprak Dönmez Çakıl, Belgin Selam, Mehmet Cıncık","doi":"10.1007/s10815-025-03458-w","DOIUrl":"https://doi.org/10.1007/s10815-025-03458-w","url":null,"abstract":"","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143709569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Incidence of cross-border reproductive care and pregnancy outcomes: a retrospective evaluation of IVF conceptions.","authors":"Suset Rodriguez, Kyara Marquez, Gemma St Louis, Arianna Vazquez, Leonardo Simonelli, Kara Lindsay, Janice Moscoso, George Roshdy Attia","doi":"10.1007/s10815-025-03449-x","DOIUrl":"https://doi.org/10.1007/s10815-025-03449-x","url":null,"abstract":"<p><strong>Purpose: </strong>This study aimed to evaluate the incidence of cross-border reproductive care (CBRC) and maternal and neonatal outcomes in pregnancies conceived through in vitro fertilization (IVF), comparing outcomes between pregnancies conceived domestically and through CBRC.</p><p><strong>Methods: </strong>A retrospective chart review was conducted on 4475 deliveries at a tertiary public hospital from February 1, 2023, to January 31, 2024. Maternal demographics, medical history, delivery outcomes, and complications were compared using statistical tests, with significance set at p < 0.05.</p><p><strong>Results: </strong>Among all deliveries, 95 were conceived via IVF. Among the IVF pregnancies, 23 (24.2%) were conceived through CBRC. While CBRC patients were older on average (40.3 vs. 37.8 years), this difference was not statistically significant (p = 0.07). The incidence of hypertensive disorders was significantly higher in CBRC pregnancies compared to domestic IVF pregnancies (69.6% vs. 37.7%, p = 0.01). Trends toward increased rates of postpartum hemorrhage (17.4% vs. 5.8%) and multiple gestations (26.1% vs. 11.6%) were also observed in the CBRC group, although these differences did not reach statistical significance. Neonatal outcomes, including rates of preterm birth, fetal growth restriction, and respiratory distress syndrome, were similar between the two groups.</p><p><strong>Conclusion: </strong>The study highlights a significantly higher percentage of hypertensive disorders among CBRC pregnancies, while other maternal and neonatal outcomes were comparable to domestic IVF pregnancies. These findings underscore the need for further research with larger sample sizes and survey-based studies to better understand patient motivations and clinical risks associated with CBRC.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143677009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From within and without the gonadal niche: Capably capacitating a gamete's destiny.","authors":"David F Albertini","doi":"10.1007/s10815-025-03448-y","DOIUrl":"https://doi.org/10.1007/s10815-025-03448-y","url":null,"abstract":"","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143663485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"m6A RNA methylation dynamics during in vitro maturation of cumulus-oocyte complexes derived from adult or prepubertal sheep.","authors":"Chiara Cosseddu, Sara Succu, Adele Frau, Francesca Mossa, Sylvia Virginie Versace, Tiziana A L Brevini, Sergio Ledda, Daniela Bebbere","doi":"10.1007/s10815-025-03444-2","DOIUrl":"https://doi.org/10.1007/s10815-025-03444-2","url":null,"abstract":"<p><strong>Purpose: </strong>N6-methyladenosine (m6A) is the most prevalent base epigenetic modification within eukaryotic mRNAs. It participates in post-transcriptional regulation, including maternal RNA maintenance and decay in mouse oocytes and during maternal-to-zygotic transition. The landscape in other mammalian species remains largely unexplored. The present work analyzed m6A dynamics in sheep cumulus oocyte complexes (COCs), during in vitro maturation. To explore potential relationships with oocyte developmental competence, a previously established model consisting of oocytes derived from adult and prepubertal sheep was adopted.</p><p><strong>Methods: </strong>m6a dynamics were analyzed in terms of m6A RNA methylation abundance in cumulus cells (CCs) by colorimetric assay and expression of key m6A methylation-related proteins (METTL3, METTL14, METTL16, VIRMA, YTHDC1, YTHDC2, YTHDF2, YTHDF3, ALKBH5, and FTO) in both cumulus cells and oocytes by real-time PCR.</p><p><strong>Results: </strong>We report the dynamics of m6A in sheep COCs, and reveal alterations in both oocytes and cumulus cells derived from prepubertal donors. These changes were observed in terms of m6A RNA methylation levels and transcript dynamics of several m6A methylation-related proteins. Notably, our study shows that dysregulations occur after IVM.</p><p><strong>Conclusion: </strong>Overall, this work describes for the first time the dynamics of m6A in sheep COCs and uncovers the involvement of m6A RNA methylation in oocyte developmental potential.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143648608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Infertility management in a patient with Swyer syndrome: a case report.","authors":"Laura Krygere, Ruta Bartasiene, Agne Kozlovskaja-Gumbriene, Egle Drejeriene","doi":"10.1007/s10815-025-03442-4","DOIUrl":"https://doi.org/10.1007/s10815-025-03442-4","url":null,"abstract":"<p><strong>Purpose: </strong>To highlight the successful use of assisted reproductive technologies in achieving pregnancy and healthy childbirth in a woman with Swyer syndrome, a rare disorder of sex development characterized by gonadal dysgenesis, elevated gonadotropin levels, oestrogen insufficiency, tall stature, delayed puberty, and primary amenorrhoea. This case highlights the efficacy of reproductive treatments for individuals with nonfunctional gonads resulting from complete gonadal dysgenesis.</p><p><strong>Methods: </strong>This report describes a woman diagnosed with Swyer syndrome at 18 years, presenting with primary amenorrhoea and delayed puberty. Following diagnosis, the patient underwent bilateral gonadectomy due to the increased risk of germ cell neoplasia and began hormone replacement therapy to address oestrogen insufficiency. At 31, the patient pursued fertility treatment, undergoing in vitro fertilisation (IVF) with donated oocytes.</p><p><strong>Results: </strong>The IVF treatment using donor oocytes led to a successful pregnancy without complications. The pregnancy culminated in the delivery of a healthy baby via caesarean section, marking a successful outcome for a patient with Swyer syndrome.</p><p><strong>Conclusion: </strong>Assisted reproductive technologies, particularly in vitro fertilisation (IVF) using oocyte donation, provide a feasible option for individuals diagnosed with Swyer syndrome to achieve parenthood. This case adds to the growing research on reproductive success in women with complete gonadal dysgenesis, emphasizing the potential for positive outcomes through specific reproductive approaches.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143648596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mitochondrial FIS1 level in cumulus cells correlates with morphological grades of human cleavage-stage embryos.","authors":"Yizhen Sima, Sanbao Shi, Zhunyuan Min, Yuning Chen, Yongning Lu, Hongying Sha, Suying Liu","doi":"10.1007/s10815-025-03431-7","DOIUrl":"https://doi.org/10.1007/s10815-025-03431-7","url":null,"abstract":"<p><strong>Purpose: </strong>Advanced-age women have a lower good-quality embryo rate (GQER) compared to young women. However, GQER varies widely within the same age group, suggesting that factors beyond age influence embryo quality. Mitochondria regulate cellular metabolism through dynamic fission and fusion alterations. Specifically, cumulus cell (CC) mitochondria regulate not only the metabolism of CCs but also of adjacent oocytes. This study aims to investigate the relationship between CC mitochondrial dynamics and oocyte developmental potential post-fertilization.</p><p><strong>Methods: </strong>CCs were collected from 183 women aged 25-45 undergoing single sperm intracytoplasmic injection-embryo transfer treatments. Samples were stratified by age into young (< 35) and advanced age (≥ 35) groups. Each group was further subdivided into high and low subgroups based on day 3 GQER. Mitochondrial morphology, dynamics, fission-fusion gene expression, and mitochondrial functions were compared among groups and subgroups.</p><p><strong>Results: </strong>Consistent with the literature, data analysis from our laboratory revealed significant variances in GQER among individuals of the same age group. Morphological analysis suggested a negative correlation between GQER and mitochondrial length in CCs (P < 0.0001, r = - 0.38). Live-cell imaging showed that both fission and fusion frequencies of CC mitochondria in the advanced-age group were lower than those in the young group (P = 0.009, P = 0.01). Additionally, within the advanced-age group, CC mitochondria from the low GQER subgroup exhibited lower fission frequency and fission-fusion ratios compared to the high GQER subgroup (P = 0.04, P = 0.01). Consequently, GQER positively correlated with mitochondrial fission-fusion ratio in CCs (P = 0.01, r = 0.44). Notably, there were no significant differences in the expression of mitochondrial fusion-related proteins (OPA1, MFN1, and MFN2) between the advanced-age and young groups or among the subgroups. However, levels of fission proteins, including FIS1 and MFF, were significantly lower in the advanced-age group compared to the young group and in the low GQER subgroup compared to their high GQER counterparts. qPCR results further indicated that fis1 and mff mRNA levels in CCs were positively correlated with GQER (P < 0.0001, r = 0.55; P = 0.0025, r = 0.41). The CCs from the low GQER subgroup exhibit a higher level of mitochondrial dysfunction.</p><p><strong>Conclusions: </strong>Mitochondrial morphology, fission-fusion balance, and fission-fusion gene expression in CCs influence early embryonic development, independent of age. Of these factors, the FIS1 level shows the most robust correlation with GQER.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143648613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preimplantation genetic testing and disorders of placental implantation: a systematic review and meta-analysis.","authors":"Isaac J Chamani, Lauren L Taylor, Hailie Ciomperlik, Timothy Dunn, Anna C Reynolds, Beatriz Varman, Karin A Fox, Laura Detti","doi":"10.1007/s10815-025-03441-5","DOIUrl":"https://doi.org/10.1007/s10815-025-03441-5","url":null,"abstract":"<p><strong>Purpose: </strong>To evaluate whether embryo biopsy for preimplantation genetic testing (PGT) during in vitro fertilization (IVF) increases the risk of disorders related to abnormal placental implantation.</p><p><strong>Methods: </strong>A systematic search of Medline, Embase, and Web of Science was performed on February 17, 2023, and identified studies on placental implantation anomalies following PGT in IVF patients. The primary outcome was placenta accreta spectrum (PAS), while secondary outcomes included placental abruption, placenta previa, preterm premature rupture of membranes (PPROM), and hypertensive pregnancy disorders. Only peer-reviewed randomized controlled trials, cohort, case-control, and cross-sectional studies in English were considered. Two reviewers independently screened citations, extracted data, and assessed study quality. Data analysis was performed using RevMan 5.4.</p><p><strong>Results: </strong>Of 282 citations retrieved by our search, 8 studies met inclusion criteria. There was no statistically significant difference in PAS risk between groups (aggregated OR 0.78, 95% CI 0.22-2.76, P = 0.70). Amongst secondary outcomes, mean BMI was lower in PGT patients (mean difference - 0.57, 95% CI - 0.98 to - 0.17, P = 0.005), and PPROM rates were higher (OR 1.29, 95% CI 1.04-1.60, P = 0.02). Other secondary outcomes showed no significant differences.</p><p><strong>Conclusion: </strong>Embryo biopsy for PGT in IVF was not associated with an increased risk of PAS. However, PGT was more commonly performed in patients with lower BMI, and an elevated risk of PPROM was observed, primarily driven by a single study. Further investigation is needed to clarify this finding and its implications.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143624894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}