Journal of Assisted Reproduction and Genetics最新文献

{"title":"Cryopreserved ovarian tissue autotransplantation in an acute myeloid leukaemia survivor following extensive minimal residual disease screening: first reported live birth in Europe.","authors":"Michelle Soares, Ingrid Segers, Michael De Brucker, Alessandra Camboni, Camille Hossay, Ileana Mateizel, Iebe De Quick, Ellen Van Moer, Dominik Selleslag, Steven Hellebaut, Marie-Madeleine Dolmans, Michel De Vos","doi":"10.1007/s10815-025-03447-z","DOIUrl":"10.1007/s10815-025-03447-z","url":null,"abstract":"<p><strong>Purpose: </strong>We report successful autotransplantation of ovarian tissue in a sterile acute myeloid leukaemia survivor after comprehensive minimal residual disease (MRD) screening.</p><p><strong>Methods: </strong>At age 18, the patient underwent ovarian tissue cryopreservation while in complete remission prior to allogeneic stem cell transplantation. Premature ovarian failure and the wish to conceive led her to request transplantation of her cryopreserved ovarian tissue 12 years later. Extensive MRD screening of frozen-thawed biopsy specimens, including histology, immunohistochemistry, PCR and 6-month xenotransplantation to immunodeficient mice, was conducted before ovarian tissue transplantation (OTT).</p><p><strong>Results: </strong>Histology and immunohistochemistry (MPO, p53, CD4, CD45) of thawed ovarian fragments showed no malignant cell contamination. Nested RT-PCR (MLL::AF9) was negative and the mouse grafted with ovarian tissue showed no disease after 6 months. The xenografted tissue was analyzed again (histology, immunohistochemistry and RT-PCR) and showed no contamination. Laparoscopic transplantation to the patient restored ovarian function after 4 months. Modified natural cycle IVF resulted in pregnancy. At 29 weeks, the patient had preterm premature rupture of membranes and delivered a healthy boy at 34.5 weeks. Three years post-transplantation, the patient remains disease-free.</p><p><strong>Conclusions: </strong>Concerns about malignant cell contamination limit cryopreserved OTT in leukemia survivors. This is the first reported European live birth in an acute myeloid leukemia survivor following frozen-thawed OTT. Safe OTT was achieved by retrieving ovarian tissue during complete remission and conducting thorough screening before transplantation. Obstetrical risks in leukemia survivors who had total body irradiation should also be thoroughly discussed during decision making before attempting motherhood.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":"1485-1490"},"PeriodicalIF":3.2,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12167415/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143788056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trustworthiness of large-scale RCT.","authors":"Raoul Orvieto","doi":"10.1007/s10815-025-03477-7","DOIUrl":"10.1007/s10815-025-03477-7","url":null,"abstract":"","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":"1739-1740"},"PeriodicalIF":3.2,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12167189/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143973078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of age, AMH, and gonadotropin dosing on IVF outcomes in fertility preservation cycles for patients less than 35 undergoing chemotherapeutic treatment: A retrospective cohort study.","authors":"Roisin M Mortimer, Andrea Lanes, Ian Waldman, Elizabeth S Ginsburg, Serene S Srouji","doi":"10.1007/s10815-025-03517-2","DOIUrl":"10.1007/s10815-025-03517-2","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the differences in total number of oocytes and MII oocytes retrieved with gonadotropin dosing in adolescent and young adult patients compared to older patients undergoing fertility preservation cycles for cancer diagnosis.</p><p><strong>Design: </strong>Retrospective cohort.</p><p><strong>Setting: </strong>Single academic institution.</p><p><strong>Patient(s): </strong>All patients less than 35 years old undergoing a fertility preservation cycle who have a cancer diagnosis, or a plan to receive gonadotoxic chemotherapy.</p><p><strong>Intervention(s): </strong>Fertility preservation cycles in patients < 22 years old (younger group) compared with those 22- < 35 years old (older group) stratifying by age, AMH, and average daily gonadotropin dosing.</p><p><strong>Main outcome measure(s): </strong>Total oocyte yield, total number of MII oocytes.</p><p><strong>Results: </strong>The younger group had 26% more oocytes retrieved and 28% more MII oocytes retrieved compared with the older group, when adjusting for BMI, chemotherapy prior to treatment and myelodysplasia (aRR 1.28, 95% CI 1.01-1.63). Starting, total, and average daily gonadotropin doses were similar between age groups. Cycles in the younger group (10.0%) were more likely to be canceled compared with the older group (2.8%) (RR 3.52, 95% CI 1.35-0.17); 35.9% of the younger group and 11.7% of the older group had chemotherapy prior to cycle start.</p><p><strong>Conclusion(s): </strong>Patients should be encouraged to undergo fertility preservation prior to receiving chemotherapy as this commonly decreases ovarian reserve and leads to a higher likelihood of cycle cancellation.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":"1443-1451"},"PeriodicalIF":3.2,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12167184/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144119320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ovarian function and response to gonadotropins after prolonged perfusion of whole ewe ovaries in a bioreactor.","authors":"Prajakta Hatekar, Panagiotis Tsiartas, Lucía de Miguel Gómez, Claudia Mateoiu, Edina Sehic, Mats Hellström, Pasquale Patrizio, Randa Akouri","doi":"10.1007/s10815-025-03432-6","DOIUrl":"10.1007/s10815-025-03432-6","url":null,"abstract":"<p><strong>Purpose: </strong>Fertility preservation for pre-pubertal girls undergoing gonadotoxic cancer treatments and women with systemic cancers at high risk for ovarian metastasis remains limited. Current options, such as ovarian cortex transplantation, risk reintroducing malignant cells. This study presents a novel approach focusing on ex vivo folliculogenesis and mature oocyte retrieval for cryopreservation, mitigating this risk.</p><p><strong>Methods: </strong>This experimental study optimized an ex vivo ovarian perfusion system in sheep, refining gonadotropin stimulation to yield mature oocytes. Eleven ovaries were divided into two experimental subgroups: Group 1 (n = 5) and Group 2 (n = 6). Both groups were perfused in a bioreactor for 4 to 8 days under distinct perfusion protocols, differing in gonadotropin administration overnight-Group 1 did not receive overnight gonadotropin stimulation, whereas Group 2 received basal gonadotropin stimulation overnight. Assessments included follicular proliferation, oocyte maturity, apoptosis, ovarian function-related gene expression, and the levels of hormones, metabolites, and electrolytes in the culture medium, compared across subgroups.</p><p><strong>Results: </strong>The protocol without overnight ovarian stimulation yielded mature MII oocytes, despite fewer secondary follicles and overexpression of the pro-apoptotic BAX gene. Conversely, ovaries with overnight stimulation yielded mostly GV-MI oocytes and exhibited reduced secondary follicle proliferation and higher HIF1A expression. Hormone levels, metabolites, and electrolytes remained stable across groups and time.</p><p><strong>Conclusions: </strong>This study is the first to report the successful harvesting of MII oocytes following extended ex vivo perfusion of intact ewe ovaries, highlighting the potential of the perfusion model to support advanced follicular development. Further investigations are warranted to elucidate underlying mechanisms and refine protocol efficiency.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":"1491-1507"},"PeriodicalIF":3.2,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12167194/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143501333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Three-dimensional culture in a bioengineered matrix and somatic cell complementation to improve growth and survival of bovine preantral follicles.","authors":"Juliana I Candelaria, Ramon C Botigelli, Carly Guiltinan, Ariella Shikanov, Anna C Denicol","doi":"10.1007/s10815-025-03497-3","DOIUrl":"10.1007/s10815-025-03497-3","url":null,"abstract":"<p><strong>Purpose: </strong>Here, we explored poly(ethylene glycol) (PEG) bioengineered hydrogels for bovine preantral follicle culture with or without ovarian cell co-culture and examined the potential for differentiation of bovine embryonic stem cells (bESCs) towards gonadal somatic cells to develop a system better mimicking the ovarian microenvironment.</p><p><strong>Methods: </strong>Bovine preantral follicles were first cultured in two-dimensional (2D) control or within PEG hydrogels (3D) and then co-cultured within PEG hydrogels with bovine ovarian cells (BOCs) to determine growth and viability. Finally, we tested conditions to drive differentiation of bESCs towards the intermediate mesoderm and bipotential gonad fate.</p><p><strong>Results: </strong>Primary follicles grew over the 10-day culture period in PEG hydrogels compared to 2D control. Early secondary follicles maintained a similar diameter within the PEG while control follicles decreased in size. Follicles lost viability after co-encapsulation with BOCs; BOCs lost stromal cell signature over the culture period within hydrogels. Induction of bESCs towards gonadal somatic fate under WNT signaling was sufficient to upregulate intermediate mesoderm (LHX1) and early coelomic epithelium/bipotential gonad markers (OSR1, GATA4, WT1). Higher BMP4 concentrations upregulated the lateral plate mesoderm marker FOXF1. PAX3 expression was not induced, indicating absence of the paraxial mesoderm lineage.</p><p><strong>Conclusions: </strong>Culture of primary stage preantral follicles in PEG hydrogels promoted growth compared to controls; BOCs did not maintain identity in the PEG hydrogels. Collectively, we demonstrate that PEG hydrogels can be a potential culture system for early preantral follicles pending refinements, which could include addition of ESC-derived ovarian somatic cells using the protocol described here.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":"1509-1523"},"PeriodicalIF":3.2,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12167402/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144110728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of pregnancy outcomes in patients with unexplained recurrent miscarriage assisted by IVF/ICSI with or without PGT-A.","authors":"Ninghua Xu, Yulin Du, Zhuolun Su, Shuang Yu, Wenjing Li, Yichun Guan, Hua Lou","doi":"10.1007/s10815-025-03438-0","DOIUrl":"10.1007/s10815-025-03438-0","url":null,"abstract":"<p><strong>Purpose: </strong>The objective of this study was to evaluate the efficacy of preimplantation genetic testing for aneuploidy (PGT-A) in reducing the incidence of early miscarriage among patients diagnosed with unexplained recurrent spontaneous abortion (URSA).</p><p><strong>Methods: </strong>This investigation was designed as a retrospective cohort study, examining patients who underwent freeze-thaw embryo transfer (FET) of single blastocysts from January 2018 to August 2023. A total of 675 FET cycles involving patients with URSA were included in the study. The primary outcome measure was the early miscarriage rate, while secondary outcome measures included the clinical pregnancy rate, ongoing pregnancy rate, and live birth rate.</p><p><strong>Results: </strong>A total of 316 patients with URSA who underwent PGT-A utilizing next-generation sequencing (NGS) technology were designated as the PGT-A group. Additionally, 359 URSA patients who underwent in vitro fertilization (IVF)/intra-cytoplasmic sperm injection (ICSI) during the same time frame were selected as the control group. Following comprehensive embryo cryopreservation, the blastocyst exhibiting the highest morphological score was chosen for the initial FET cycle in both groups. The pregnancy outcomes between the two groups were subsequently compared. In patients with URSA, the application of PGT-A was associated with improved clinical pregnancy rates (64.2% vs. 45.7%; aOR, 2.012; 95% CI, 1.303 to 3.108; P = 0.002), ongoing pregnancy rates (53.2% vs. 34.0%; aOR, 2.121; 95% CI, 1.379 to 3.260; P = 0.001), and live birth rates (51.3% vs. 32.9%; aOR, 2.019; 95% CI, 1.316 to 3.097; P = 0.001). In patients aged 38 years and older with unexplained recurrent miscarriages, PGT-A not only increased the rate of ongoing pregnancies (50.0% vs. 17.5%; aOR, 4.325; 95% CI, 1.31 to 14.281; P = 0.016) and live birth rates (46.7% vs. 17.5%; aOR, 3.684; 95% CI, 1.141 to 11.893; P = 0.029), but also significantly reduced the rate of early miscarriage (16.7% vs. 40.0%; aOR, 0.098; 95% CI, 0.01 to 0.956; P = 0.046).</p><p><strong>Conclusions: </strong>PGT-A has been demonstrated to enhance clinical pregnancy rates, ongoing pregnancy rates, and live birth rates in patients experiencing unexplained recurrent miscarriages. Furthermore, the implementation of PGT-A significantly reduced the rate of early miscarriage among older patients aged 38 years and above.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":"1679-1687"},"PeriodicalIF":3.2,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12167211/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143573097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Up-regulated microRNAs in blastocoel fluid of human implanted embryos could control circuits of pluripotency and be related to embryo competence.","authors":"Rosalia Battaglia, Angela Caponnetto, Carmen Ferrara, Anna Fazzio, Cristina Barbagallo, Michele Stella, Davide Barbagallo, Marco Ragusa, Maria Elena Vento, Placido Borzì, Paolo Scollo, Luca Carli, Michael Feichtinger, Evangelia Kasapi, Elias Tsakos, Simone Palini, Wojciech Sierka, Basilio Pecorino, Maria Rosaria Campitiello, Carlo Ronsini, Michele Purrello, Domenico Valerio, Salvatore Longobardi, Thomas D'Hooghe, Cinzia Di Pietro","doi":"10.1007/s10815-025-03457-x","DOIUrl":"10.1007/s10815-025-03457-x","url":null,"abstract":"<p><strong>Purpose: </strong>The paper aims to investigate the biological role of microRNAs secreted by preimplantation embryo into the blastocoel fluid and to detect a distinctive molecular signature for identifying embryos with the highest implantation potential.</p><p><strong>Methods: </strong>We carried on a multicenter retrospective study involving five European IVF centers. We collected 112 blastocoel fluid samples from embryos on day 5 post-fertilization, cultured individually, along with data on blastocyst grade and embryo transfer outcomes. Using a custom TLDA Array, we compared the expression levels of 89 miRNAs between 33 fluids from high-quality implanted embryos and 30 fluids from high-quality not-implanted embryos. Expression differences were assessed using SAM and t-test. Additionally, correlation and function enrichment analysis and network construction were conducted to identify the biological roles of deregulated microRNAs.</p><p><strong>Results: </strong>We identified six up-regulated microRNAs in the blastocoel fluid from implanted embryos, significantly and positively correlated across all samples (r ≥ 0.7; P ≤ 0.05). They could take part in pluripotency circuits, regulating and being regulated by transcription factors associated with stemness, cell growth, and embryo development. The ROC curve analysis confirmed the potential of these miRNAs as implantation classifiers.</p><p><strong>Conclusion: </strong>The six miRNAs up-regulated in blastocoel fluid from implanted embryos may represent a functional molecular signature for evaluating blastocyst quality and identifying the most competent embryos. Their evaluation associated with non-invasive preimplantation genetic testing, integrating epigenetic and genomic analyses, could enhance implantation grade and allow for identification of the euploid embryo not able to implant.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":"1635-1649"},"PeriodicalIF":3.2,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12167203/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143719385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ovulatory cycle frozen embryo transfer yields similar pregnancy outcomes to programmed transfer while avoiding painful injections.","authors":"Katie White, Jessica Cheung, Prachi Godiwala, Reeva Makhijani, Allison Bartolucci, Claudio Benadiva, Lawrence Engmann, Daniel Grow","doi":"10.1007/s10815-025-03446-0","DOIUrl":"10.1007/s10815-025-03446-0","url":null,"abstract":"<p><strong>Purpose: </strong>Optimal endometrial preparation is essential for the transfer of a euploid embryo. Recent works have shown advantages of ovulatory cycle frozen-thawed embryo transfer (Ovu-FET) for simplicity, implantation rate, and reduction of hypertensive disorders of pregnancy; however, few women over age 40 years were included in these studies. This study compared pregnancy outcomes in patients aged 40-45 years between Ovu-FET and programmed cycle FET (PC-FET).</p><p><strong>Methods: </strong>Retrospective cohort study in a university-affiliated fertility center. Two hundred eighty-seven FET cycles with euploid embryo transfer between 2015 and 2022 were included, with 116 cycles utilizing the PC-FET and 171 the Ovu-FET protocol. The use of letrozole was permitted for patients with irregular cycles. The primary outcome was ongoing pregnancy rate with secondary outcomes including pregnancy rate, clinical pregnancy rate, biochemical rate, and clinical loss rate. Multivariate regression was utilized to adjust for potential covariates.</p><p><strong>Results: </strong>The ongoing pregnancy rates were similar between the Ovu-FET versus PC-FET groups (62.6% vs. 58.6%, P = 0.50). Pregnancy rates (77.2% vs. 82.8%, P = 0.25) and clinical pregnancy rates (69.5% vs. 68.1%, P = 0.79) were also similar. Clinical loss rates were similar between the two groups (7.0% vs. 9.5%, P = 0.41). Biochemical loss rates were slightly lower for the Ovu-FET versus PC-FET groups (7.6% vs. 14.7%, P = 0.06) without reaching statistical significance.</p><p><strong>Conclusion: </strong>Our study supports the use of Ovu-FET in women aged 40 years and older undergoing euploid embryo transfers. The results should strengthen confidence in the use of Ovu-FET for older patients.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":"1663-1670"},"PeriodicalIF":3.2,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12167218/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143752945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Congenital anomalies observed in children conceived through assisted reproductive technology-a systematic review and meta-analysis.","authors":"Paripoorna Bhat, Vijay Shree Dhyani, Vani Lakshmi, Shubhashree Uppangala, Satish Kumar Adiga, Prashanth Adiga, Pratap Kumar, Aditi Gupta","doi":"10.1007/s10815-025-03454-0","DOIUrl":"10.1007/s10815-025-03454-0","url":null,"abstract":"<p><p>Congenital anomalies (CAs) are a leading cause of perinatal and child mortality. With the increasing use of assisted reproductive technology (ART), there is a growing need for research on the health outcomes of children conceived through ART. This systematic review was performed to assess the incidence of CAs in ART-conceived children compared to those conceived naturally. This review followed the PRISMA 2020 guidelines and was registered with PROSPERO. A total of 113 studies were included in this meta-analysis, comprising 768,929 children in the ART group and 40,709,337 children in the control group which comprised spontaneously conceived (SC) children. The primary findings indicated that ART-conceived children have a marginally higher risk of CAs compared to the control group. Subgroup analyses showed that children conceived via ICSI, Day 3 transfer, and fresh embryo transfer (ET) had a slightly higher risk of CAs than those conceived via IVF, Day 5 transfer, or frozen embryo transfer (FET). The study highlights a slightly increased incidence of CAs among ART-conceived children over SC children, underscoring the importance of improving ART methods and closely monitoring the health of these children to reduce the risk of CAs.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":"1547-1565"},"PeriodicalIF":3.2,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12167209/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143752937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spindle observation aids decision-making for early rescue ICSI.","authors":"Yang Gao, Jiahong Zhu, Shun Xiong, Jiang Wang, Xiangwei Hao, Haiyuan Liao, Wei Han, Junxia Liu, Guoning Huang","doi":"10.1007/s10815-025-03474-w","DOIUrl":"10.1007/s10815-025-03474-w","url":null,"abstract":"<p><strong>Objective: </strong>To investigate whether spindle visualization using polarized light microscopy (PLM) can assist in identifying unfertilized oocytes prior to early rescue intracytoplasmic sperm injection (R-ICSI).</p><p><strong>Methods: </strong>This retrospective cohort study included 356 early R-ICSI cycles, of which 24 were excluded from the analysis. The remaining 332 cycles were divided into study group and control group based on whether spindle observation had been performed before early R-ICSI. The fertilization rate, embryo development, and clinical outcome were compared between the two groups.</p><p><strong>Results: </strong>Of the 356 participants included, 332 completed follow-up analysis. The study group demonstrated a significantly higher normal fertilization rate (82.55% vs. 71.49%, P < 0.01) and a lower 3PN rate (3.17% vs. 8.89%, P < 0.01) after following R-ICSI. For oocytes without visible spindles in the study group or those with fragmented polar bodies in the control group, in vitro fertilization (IVF) was performed, resulting in a higher normal fertilization rate in the study group (53.37% vs. 36.43%, P < 0.01). However, there was no significant difference between the two groups in terms of embryo development and implantation potential. In the high-age subgroup (over 35 years old), the normal cleavage rate was significantly higher in the spindle examination group compared to the non-observation group (38.60% vs. 22.47%, P < 0.05).</p><p><strong>Conclusion: </strong>Utilizing PLM for spindle observation can effectively identify oocytes suitable for early R-ICSI to improve fertilization outcomes and optimize embryo development in women over 35 years old.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":"1625-1633"},"PeriodicalIF":3.2,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12167208/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143803394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}