Journal of Assisted Reproduction and Genetics最新文献

{"title":"Hepatitis B virus impacts embryonic development and methylation of maternal genes in assisted reproductive technology patients.","authors":"Xia Liu, Shixiang Dong, Yu Ding, Jinjin Li, Jingyuan Wang","doi":"10.1007/s10815-024-03359-4","DOIUrl":"https://doi.org/10.1007/s10815-024-03359-4","url":null,"abstract":"<p><strong>Purpose: </strong>In China, the prevalence of hepatitis B virus (HBV) infection among infertile couples is a significant clinical problem. It is necessary to determine the effect of HBV infection on embryo development.</p><p><strong>Methods: </strong>The 4301 fresh cycles and 5763 frozen embryo transfer (FET) cycles were grouped according to the couple with or without HBV infection. The embryo fertilization rate, cleavage rate, transplantable embryo rate, and rate of high-quality embryos were analysed. The methylation status of maternal antigen that embryos require (MATER), zygote arrest 1 (ZAR1) and growth differentiation factor 9 (GDF9) genes in the peripheral blood of assisted reproductive technology (ART) women was detected by methylation-specific polymerase chain reaction (MSP).</p><p><strong>Results: </strong>The pregnancy rate of the female HBV-positive group was significantly lower than that of the HBV-negative group. The fertilization rate of intracytoplasmic sperm injection (ICSI) cycles in the male HBV-positive group was significantly lower than that of the male HBV-negative group. There were no differences in biochemistry or clinical pregnancy rates among the FET groups. The promoter methylation of GDF9 in HBV-positive ART women was higher than that in HBV-negative ART women, and that of ZAR1 in HBV-positive ART women was lower than that in HBV-negative ART women.</p><p><strong>Conclusion: </strong>It was a detrimental effect of HBV infection on in vitro fertilization (IVF) and ICSI treatment outcomes in women. The HBV infection was associated with the maternal genes promoting methylation.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142894611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"FXYD1 was identified as a hub gene in recurrent miscarriage and involved in decidualization via regulating Na/K-ATPase activity.","authors":"Jie Gan, Long Yang, Shu-Han Yang, Wen-Wen Gu, Yan Gu, Yan Shi, Jia-Xin Shi, Hao-Ran Xu, Ya-Wei Xin, Xuan Zhang, Jian Wang","doi":"10.1007/s10815-024-03363-8","DOIUrl":"https://doi.org/10.1007/s10815-024-03363-8","url":null,"abstract":"<p><strong>Purpose: </strong>Recurrent miscarriage (RM) is a distressing and complicated adverse pregnancy outcome. It is commonly recognized that insufficient decidualization could result in RM, but the molecular mechanisms of decidual impairment are still not fully understood. Thus, this study aimed to identify novel key genes potentially involved in RM and explore their roles played in endometrial decidualization.</p><p><strong>Methods: </strong>Initially, a combinative analysis of decidual and mid-secretory endometrial transcriptomes was performed to discover hub genes involved in the etiology of RM. And the expression levels of hub genes were evaluated in both primary decidual stromal cells (DSCs) and decidual tissues. Subsequently, the immortalized human endometrial cell line, T-HESCs, was used to investigate whether FXYD1 overexpression affects decidualization by regulating Na/K-ATPase activity.</p><p><strong>Results: </strong>FXYD domain containing ion transport regulator 1 (FXYD1) was identified as a hub gene in the pathogenesis of RM through various bioinformatic methods. Abnormally increased FXYD1 expression was observed in DSCs and decidual tissues from RM patients compared to that of the normal group. Furthermore, in vitro decidualization was obviously inhibited by the overexpression of FXYD1. Additionally, Na/K-ATPase activity was significantly elevated during decidualization, whereas overexpression of FXYD1 reduced Na/K-ATPase activity. Bufalin, a Na/K-ATPase inhibitor, showed an effectively inhibitory effect on decidualization.</p><p><strong>Conclusions: </strong>Collectively, FXYD1 was discovered as a hub gene associated with RM, and its expression levels in RM patients were significantly upregulated. Increased FXYD1 expression might lead to decidualization defects by reducing Na/K-ATPase activity, of which presented a novel prospective treatment target for RM.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142894605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An isolated 17,20-lyase deficiency patient achieved a successful live birth after in vitro fertilization: a case report and narrative review.","authors":"Zhijin Hou, Fangjie Jiang, Wenan Li, Shenglan Fu, Yushi Meng","doi":"10.1007/s10815-024-03366-5","DOIUrl":"https://doi.org/10.1007/s10815-024-03366-5","url":null,"abstract":"<p><strong>Purpose: </strong>This study aimed to investigate the genetic etiology in an infertile patient presenting with consistently elevated progesterone levels.</p><p><strong>Methods: </strong>Genomic DNA was extracted from the patient's blood sample and subjected to whole-genome sequencing (NGS) using the Illumina NovaSeq platform. Bioinformatic analyses were conducted to identify single nucleotide variants (SNVs) and insertion-deletion mutations (Indels) potentially associated with the patient's clinical phenotype. These variants were subsequently validated using Sanger sequencing. To further assess the functional implications of these genetic variants, three-dimensional protein structure simulations and substrate molecular docking analyses were performed on the variant proteins.</p><p><strong>Results: </strong>A point mutation, c.1096 G > T (p.Val366Leu), was identified in the patient's CYP17A1 gene. Compared to the wild type, the mutant exhibited no significant changes in the overall or local three-dimensional structure, and molecular docking analysis showed no notable difference in binding energy. A literature review indicated that this mutation site is located in the region where the CYP17A1 enzyme interacts with cytochrome b5 (Cyt b5).</p><p><strong>Conclusions: </strong>We report, for the first time, that a novel mutation in the CYP17A1 gene in an infertile woman may have led to isolated 17,20-lyase deficiency. The patient successfully achieved pregnancy and delivered a healthy baby through in vitro fertilization (IVF).</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142894594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transvaginal ovarian drilling for polycystic ovary syndrome prior to in vitro fertilization dramatically improves embryo yield, implantation, and ongoing pregnancy rates.","authors":"Sarah C Rubin, Moses Bibi, Andrzej Breborowicz, Patricia Chau, Martin D Keltz","doi":"10.1007/s10815-024-03362-9","DOIUrl":"https://doi.org/10.1007/s10815-024-03362-9","url":null,"abstract":"<p><strong>Purpose: </strong>To evaluate the effect of transvaginal ovarian drilling (TVOD) on IVF outcomes in subjects with clomiphene-resistant PCOS and a history of IVF failure.</p><p><strong>Methods: </strong>Between 2008 and 2011, 19 subjects with sonographically PCOS and a history of failure to ovulate to high-dose clomiphene citrate were prospectively followed and underwent TVOD at a university hospital-based IVF program.</p><p><strong>Results: </strong>In 15 subjects who underwent 30 fresh paired IVF cycles TVOD resulted in a significantly higher number of oocytes retrieved (7.2 ± 5.9 vs. 13.2 ± 5.9, p = 0.007), mature oocytes retrieved (4.6 ± 3.4 vs. 9.5 ± 5.2, p = 0.002), embryos (3.8 ± 2.7 vs. 8.5 ± 4.5, p = 0.0002), and blastocysts (0.73 ± 1.33 vs. 2.77 ± 2.7, p = 0.037). Among all IVF cycles, 19 subjects underwent 23 fresh IVF cycles prior to TVOD and 21 fresh cycles within 6 months following TVOD lead to higher implantation (0.10 vs. 0.37, p = 0.001), clinical pregnancy (17.4% vs. 61.9%, p = 0.002), and ongoing pregnancy rates (4.4% vs. 47.6%, p = 0.014).</p><p><strong>Conclusion: </strong>In this prospective cohort study, TVOD appears to markedly improve IVF outcomes in subjects with clomiphene resistant PCOS and a history of IVF failure.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142882171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploration of ferroptosis-related biomarkers with prognostic capability in RIF based on WGCNA.","authors":"Tingting Zhou, Qian Zhang, Wenhao Yu, Yuqian Cui, Junhao Yan, Tianxiang Ni, Xiaohua Fu, Junwei Li","doi":"10.1007/s10815-024-03370-9","DOIUrl":"https://doi.org/10.1007/s10815-024-03370-9","url":null,"abstract":"<p><strong>Purpose: </strong>To explore the association of ferroptosis with repeated implantation failure (RIF) and prognostic capability of ferroptosis-related genes.</p><p><strong>Methods: </strong>Data in GSE106602 from the GEO database were used for gene co-expression network construction to confirm ferroptosis-related genes compared to gene sets that were downloaded from FerrDB. Then these genes were analyzed for functional enrichment and validated using endometrium samples from our center. ImplantScore and ROC curve were constructed for prognostic correlation analysis.</p><p><strong>Results: </strong>We observed that ferroptosis probably participated in RIF according to bioinformatics analysis on a gene set which exhibited a strong association with RIF from WGCNA. Fifty-four ferroptosis-related genes in the gene set were subsequently verified, and the PPI network was established for underlying interactions among them. There were 23 hub genes with differential expression in RIF and six of them (PML, LCN2, PRKAA1, BACH1, SLC7A11, and CAMKK2) showed significant correlation with implantation outcomes using samples collected from our center. Therefore, we combined the six genes and constructed an ImplantScore whose AUC reached 0.891, higher than the AUC of each single gene, respectively. ImplantScore of six genes with down-regulated expression in the group with failed implantation were much lower than that with successful outcome.</p><p><strong>Conclusion: </strong>Our results demonstrated the potential prognostic functions of ferroptosis-related biomarkers in RIF, which will provide novel perspectives for further research and clinical applications.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142882153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The intricate \"ART\" of ICSI.","authors":"Olena M Kocur, Philip Xie, Stephanie Cheung, Lily Ng, Angela De Jesus, Zev Rosenwaks, Gianpiero D Palermo","doi":"10.1007/s10815-024-03322-3","DOIUrl":"https://doi.org/10.1007/s10815-024-03322-3","url":null,"abstract":"<p><p>This manuscript explores the intricacies and nuances of the Intracytoplasmic Sperm Injection (ICSI) procedure, drawing on insights from three decades of experience at a specialized center managing numerous cases of male factor infertility. Our center is comprised of an embryology laboratory, an andrology and assisted fertilization laboratory, and a Preimplantation Genetic Testing for Aneuploidy (PGT-A) laboratory, each fostering specialized expertise independently. Collaboration among these laboratories, alongside reproductive physicians and urologists, ensures comprehensive feedback and optimal care for patients undergoing infertility treatment. The manuscript specifically focuses on the andrology laboratory's pivotal role in evaluating and treating infertile patients, highlighting critical preparations for the ICSI procedure, and the key considerations essential to its successful implementation, including the selection of the ideal spermatozoon, oocyte dysmaturity, proper equipment, and most importantly the execution of the procedure itself.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142877292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of SARS-CoV-2 on the male reproductive tract: insights from semen analysis and cryopreservation.","authors":"Silvia Giugliano, Alessandro M Mozzarelli, Annalisa Navarra, Gabriele De Simone, Maria Rescigno, Paolo E Levi-Setti, Elena Albani","doi":"10.1007/s10815-024-03321-4","DOIUrl":"https://doi.org/10.1007/s10815-024-03321-4","url":null,"abstract":"<p><strong>Purpose: </strong>Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus behind the COVID-19 pandemic, affects multiple organs, including the male reproductive system. While viral infections can harm male fertility through cytokine storms, the effects of SARS-CoV-2 on fertility are still unclear. Thus, this study aimed to examine the persistence of viral RNA and inflammatory responses in semen following SARS-CoV-2 infection and the safety of conventional freezing and vitrification techniques.</p><p><strong>Methods: </strong>Semen samples from 20 patients were collected 3 months post-SARS-CoV-2 infection. Samples underwent freezing and vitrification. Molecular and cellular analysis separated seminal plasma and pellets. Flow cytometry characterized immune cells. Viral RNA was extracted from plasma and sperm, followed by RT-qPCR. Cytometric Bead Array measured cytokine levels.</p><p><strong>Results: </strong>Angiotensin-converting enzyme 2 (ACE2) and transmembrane serine protease 2 (TMPRSS2) receptors were detected in both plasma and sperm fractions. Five patients exhibited viral RNA-dependent RNA polymerase, indicating potential persistence. Elevated inflammatory cytokines in plasma implied persistent inflammation affecting sperm vitality. Immune cells associated with viral clearance were identified in semen, correlating with receptor expression and cytokines. Both conventional freezing and vitrification were found safe procedures for preserving male fertility.</p><p><strong>Conclusions: </strong>Our study highlights the impact of SARS-CoV-2 on male reproductive health, emphasizing the persistence of viral entry receptors, potential viral RNA presence, the inflammatory environment, and the involvement of immune populations in the male reproductive tract post-infection. Importantly, we confirm the safety of conventional freezing and vitrification techniques for preserving male fertility in assisted reproductive technology programs amidst the COVID-19 pandemic.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142854298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A review of the association between in vitro fertilization and children's neurocognitive development.","authors":"Nicholas Aderinto, Emmanuel Kokori, Gbolahan Olatunji, Ikponmwosa Jude Ogieuhi, Israel Charles Abraham, Bonaventure Michael Ukoaka, Adetola Emmanuel Babalola, Yewande Abigail Adebayo, Chimezirim Ezeano, Oluwaseun Oyewo, Ganiyat Adekemi Adeshina","doi":"10.1007/s10815-024-03348-7","DOIUrl":"https://doi.org/10.1007/s10815-024-03348-7","url":null,"abstract":"<p><p>This review investigated the current research on the association between in vitro fertilization and children's neurocognitive development. Twenty studies were analyzed, encompassing over 23,000 children conceived through IVF, and compared to those conceived naturally. The findings on overall cognitive function were mixed, as measured by IQ. Some studies showed no significant differences between IVF and naturally conceived children, while others suggested slight variations. There is emerging evidence that IVF might correlate with specific cognitive domains like language and motor skills, although more research is needed. Several established factors, including maternal age, education level, and birth weight, are associated with children's cognitive development, regardless of conception method. Future research should explore how these factors interact with IVF and investigate a broader range of cognitive domains. Socioeconomic background and parental involvement are essential considerations for understanding a child's developmental trajectory. The inconclusive nature of some findings highlights the need for further research with larger sample sizes, more extended follow-up periods, and robust methodologies. This research has potential implications for parents considering IVF or ICSI, healthcare professionals providing guidance, and future efforts to tailor support systems for children conceived through assisted conception techniques. Open communication about the current state of knowledge and responsible communication of research findings is crucial.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142836609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Infertility care and physician trainees: a survey on elective fertility preservation.","authors":"Rajesh Reddy, Mackenzie N Naert, Miriam A Andrusier, Andrea Pelletier, Mark D Hornstein","doi":"10.1007/s10815-024-03355-8","DOIUrl":"https://doi.org/10.1007/s10815-024-03355-8","url":null,"abstract":"<p><strong>Purpose: </strong>Female physicians experience a higher rate of infertility than the general population, are more likely to delay childbearing in training and use assisted reproductive technologies. Little is known about how physician trainees use infertility services or perceive an employer-sponsored fertility preservation benefit. This study assessed physician trainee knowledge of and experiences with infertility and gauged interest in an employer-sponsored fertility preservation benefit.</p><p><strong>Methods: </strong>A 44-question survey was offered from December 2021 to March 2022 to all physician trainees within a single academic hospital system. Descriptive statistics were performed to discern patterns of infertility care.</p><p><strong>Results: </strong>Of 2495 invited trainees, 550 (22.0%) responded, of whom 11.6% had experienced infertility, 15.1% had undergone a consultation, and 8.4% had received treatment (90.0% and 67.2% of those who had experienced infertility, respectively). Almost half (48.8%) of respondents had considered fertility preservation, of which only 6.3% had used this service (32.8% among those who had experienced infertility).</p><p><strong>Conclusion: </strong>Trainees experience considerable rates of infertility, are concerned by fertility preservation costs, and support an employer-sponsored benefit.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142846767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The emotions, concerns and reactions of sperm donor candidates to their rejection by a sperm bank.","authors":"Guido Pennings, Emilie Lassen, Josephine G Lemmen, Lina Thirup, Allan Pacey, Anne-Bine Skytte","doi":"10.1007/s10815-024-03357-6","DOIUrl":"10.1007/s10815-024-03357-6","url":null,"abstract":"<p><strong>Purpose: </strong>To explore the emotions, concerns and reactions of sperm donor candidates to their rejection by the sperm bank.</p><p><strong>Methods: </strong>An online questionnaire was administered at Cryos International in the USA and Denmark to sperm donor candidates who had received notification that they were ineligible to donate.</p><p><strong>Results: </strong>A total of 101 rejected donor candidates completed the questionnaire. The majority were disappointed and surprised by the rejection but only a relatively small group had concerns about their health, fertility or manhood. More than half of them (54.5%) were satisfied with the way they were informed of their ineligibility while 17.8% were neutral, and 27.7% were dissatisfied. Approximately half (47.5%) thought that the information provided to them was sufficient while 24.8% were neutral and one in four (27.8%) considered it as insufficient. The most frequent suggestions for improvement were to provide an information leaflet specific to the reason of rejection, offer personal counselling and explain better what the implications are for them.</p><p><strong>Conclusion: </strong>There is room for improvement in the information provided to donor candidates about the reason for their rejection. Although some information is already currently provided, a minority of donor candidates considered this to be insufficient. However, rejected candidates do not request more information on their own initiative. Routine provision of an information sheet specific to each reason for rejection may improve the experience of these men.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142828719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}