Journal of Assisted Reproduction and Genetics最新文献

{"title":"Functional interplay between endoplasmic reticulum stress and mitochondrial activity in a post-ovulatory aging model of mouse oocytes.","authors":"Fumihiro Nakamura, Isao Takehara, Saki Hine, Midori Saito, Jun Matsukawa, Michi Nishi, Satoru Nagase","doi":"10.1007/s10815-025-03785-y","DOIUrl":"10.1007/s10815-025-03785-y","url":null,"abstract":"<p><strong>Purpose: </strong>To investigate whether targeting the ER-mitochondria axis can improve embryonic development in a post-ovulatory aging (POA) model by evaluating organelle-specific modulation.</p><p><strong>Methods: </strong>Fresh oocytes were collected immediately after ovulation, and post-ovulatory aged oocytes were obtained using a mouse model. Fresh oocytes were treated with thapsigargin (an ER stress inducer) or CCCP (a mitochondrial inhibitor), and ER stress (GRP78 fluorescence) and mitochondrial superoxide status (MitoSOX™ fluorescence) were assessed. Aged oocytes were treated with salubrinal (an ER stress modulator), 5-aminolevulinic acid (5-ALA, a mitochondrial modulator), or both. Embryonic development was evaluated via blastocyst formation and cell death rates.</p><p><strong>Results: </strong>In fresh oocytes, CCCP increased GRP78 fluorescence, and thapsigargin increased MitoSOX fluorescence, consistent with bidirectional ER-mitochondria stress propagation. In POA oocytes, salubrinal reduced GRP78 fluorescence but not MitoSOX, whereas 5-ALA reduced MitoSOX fluorescence but not GRP78; the combined treatment showed no additive effects on either proxy marker. Despite the lack of additivity at the marker level, blastocyst formation increased and apoptosis decreased in all treatment groups, with no additional benefit of combined treatment compared with single agents.</p><p><strong>Conclusions: </strong>In this POA model, ER-mitochondria coupling detected in freshly ovulated oocytes appeared attenuated. While our single-marker readouts (GRP78 and MitoSOX) are limited proxies, independent targeting of either organelle modestly improved blastocyst formation. These data support a hypothesis-generating framework for future studies in maternal aging models. Results were obtained under 20% O₂ culture and may be conservative relative to physiologic (5%) O₂ conditions.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":"851-864"},"PeriodicalIF":2.7,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12982706/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145850241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transvaginal ovarian drilling prior to a second IVF cycle may improve the rate of euploidy in patients with polycystic ovarian syndrome when compared to controls.","authors":"Moses Bibi, Sarah Rubin, Kaleb Noruzi, Rachel Stern, Shmuel Sashitzky, Adi Steinhart, Martin D Keltz","doi":"10.1007/s10815-025-03778-x","DOIUrl":"10.1007/s10815-025-03778-x","url":null,"abstract":"<p><strong>Purpose: </strong>To assess the impact of transvaginal ovarian drilling (TVOD) on euploidy rates following repeat in vitro fertilization (IVF) in subjects with polycystic ovarian syndrome (PCOS).</p><p><strong>Materials and methods: </strong>A single institution retrospective cohort study between January 2017 and December 2024, all patients with PCOS, as confirmed by Rotterdam criteria, who had TVOD performed prior to a repeat IVF cycle and underwent PGT-A in both the prior and repeat cycles were included and compared to a well-matched control. The primary outcome was the number of transferable embryos per cycle. Secondary outcomes included: blastocyst yield, euploid yield, and rates of aneuploidy.</p><p><strong>Results: </strong>Eighteen subjects met criteria in our study time frame and were compared to 57 control subjects. The mean age for the TVOD and control groups was 35.3 ± 4.6 and 36.5 ± 4.4 respectively (p = 0.34). TVOD was associated with a doubling of blastocyst yield, a sixfold increase in the yield of euploid blasts, and a nearly fourfold decrease in the percentage of aneuploid blasts. When compared to controls, TVOD resulted in a significant improvement in the yield of euploid embryos, from + 1.3 to + 2.4 (p = 0.01), and transferable embryos from + 1.5 to + 3.9 (p = 0.001). TVOD also resulted in a decrease in the percentage of aneuploid embryos from - 8.5 to - 49% (p < 0.001).</p><p><strong>Conclusion: </strong>TVOD appears to have positively impacted the yield of transferable embryos and euploid embryos in patients with PCOS when compared to a well-matched control.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":"779-787"},"PeriodicalIF":2.7,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12982796/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145933477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acute anomalous high-temperature exposure impacts on mouse ovaries.","authors":"Luhan T Zhou, Dilan Gokyer, Sophia Akinboro, Francesca E Duncan, Elnur Babayev","doi":"10.1007/s10815-026-03801-9","DOIUrl":"10.1007/s10815-026-03801-9","url":null,"abstract":"<p><strong>Purpose: </strong>The purpose of this study is to assess the impact of acute anomalous high-temperature exposure on mouse ovaries, hormone profiles, estrous cyclicity, and folliculogenesis.</p><p><strong>Methods: </strong>Reproductively young CD-1 mice (6-12 weeks old) were housed in temperature-adjustable chambers under control (28 °C) or heat stress conditions (35 °C) for 1 or 4 consecutive days. Ovaries were collected either immediately after exposure or after a 3-week delay period.</p><p><strong>Results: </strong>Body weights remained unchanged across groups. Ovary weight was significantly reduced immediately following 4 days of heat exposure, while ovary length trended toward a decrease. Serum hormones-AMH, FSH, and progesterone-were not significantly different between groups. All animals cycled at least twice during a 2-week monitoring period. Follicle counts across all follicle stages were also not significantly different between groups.</p><p><strong>Conclusion: </strong>Short-term exposure to elevated temperature (35 °C) leads to a transient reduction in ovary weight without altering hormone levels, cyclicity, or folliculogenesis. These findings suggest that acute heat stress may primarily affect the ovarian stroma, warranting further investigation into its structural and functional consequences.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":"997-1005"},"PeriodicalIF":2.7,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12982804/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145998257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Joint effects of IVF and cancer history on birth outcomes.","authors":"Abby L Chen, Shufeng Li, Valerie L Baker, Philip J Lupo, Hazel B Nichols, Michael L Eisenberg, Barbara Luke","doi":"10.1007/s10815-026-03803-7","DOIUrl":"10.1007/s10815-026-03803-7","url":null,"abstract":"<p><strong>Purpose: </strong>To examine how live birth rates, preterm birth, and major birth defects are affected by parental cancer history and conception method.</p><p><strong>Methods: </strong>IVF births were identified by linking the Society for Assisted Reproductive Technology Clinic Outcomes Reporting System to birth certificates in three States. For each IVF-conceived birth, the subsequent 10 naturally conceived births (from birth certificates) created the comparison group. Parental cancer history was identified by linkage to state cancer registries. Preterm birth (PTB) was defined from birth certificates; birth defects (BD) from state BD registries. Adjusted odds ratios (aOR) and 95% confidence intervals (CI) for PTB and BDs were calculated with logistic regression.</p><p><strong>Results: </strong>Overall, 30.5% of female cancer survivors who underwent IVF had a live birth. Of 814,658 total births, 9.1% were PT and 4.4% had BDs. IVF was associated with increased risk of PTB and major BDs regardless of parental cancer history. Maternal, but not paternal, cancer history was associated with an increased risk of PTB. No increase in BDs was observed with parental cancer history alone. Risks of both PTB and BDs were highest among multiple births.</p><p><strong>Conclusions: </strong>IVF use was associated with increased risk of PTB and major BDs. Parental cancer history did not elevate the risk of BDs, which remains low. Based on these data, cancer survivors attempting to conceive with or without IVF can be counseled that the magnitude of risk for PTB and major BD was similar for cancer survivors compared with those who conceive without a parental cancer history.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":"911-922"},"PeriodicalIF":2.7,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12982715/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146018692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Validation and clinical study of single-step vitrification combined with single-step warming of human blastocysts.","authors":"Jan Gunst, Hubert Joris, Matthijs Vynck, Kristof Godderis, Martine Vercammen, Sylvie Roggeman, Arne van de Vijver","doi":"10.1007/s10815-025-03790-1","DOIUrl":"10.1007/s10815-025-03790-1","url":null,"abstract":"<p><strong>Purpose: </strong>This study assesses the feasibility of single-step vitrification and warming (SSV/SSW) for human blastocysts. Previous animal study models have shown possibilities of shorter cryopreservation methodologies by cryoprotectant and temperature adjustments. The main research question investigates whether SSV/SSW can serve as an effective alternative to stepwise vitrification (SWV) and warming procedures, ensuring comparable embryological and clinical outcomes.</p><p><strong>Method: </strong>The study was conducted at a single center. Validation of SSV combined with SSW assessing survival, re-expansion, and development after 24 h was performed using research blastocysts. Cryoprotectant (CPA) levels were measured in three different SSW sucrose solutions after SSV or SWV. We introduced the valid method in the clinic and report laboratory and clinical outcomes of 333 SWV/SSW against 568 SSV/SSW blastocyst transfers.</p><p><strong>Results: </strong>During validation, SSV at 37 °C, compared to SWV, combined with 1 M sucrose SSW demonstrated comparable collapsing (89.6% vs. 98.1%) and re-expansion outcomes (97.9% vs. 88.7%). Extracellular CPA analysis (79.3 mg/L vs. 127.3 mg/L; p < 0.001) indicated significantly lower intracellular uptake following SSV compared to SWV. Clinical data showed significantly lower rates of collapsing (67.8% vs. 92.5%, p < 0.001) and re-expansion (72.0% vs. 89.5%, p < 0.001) after SSV compared to SWV, but similar rates of ongoing pregnancy (25.9% vs. 26.1%) and secondary clinical outcomes.</p><p><strong>Conclusion: </strong>SSV combined with SSW is a feasible, operationally efficient alternative to SWV and warming procedures open to further optimize the collapse status before or during vitrification. The method reduces handling time, CPA exposure, and operator-related errors while maintaining ongoing pregnancy rates.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":"837-850"},"PeriodicalIF":2.7,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12982725/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146180121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ethical and logistical considerations for the development of institutional posthumous sperm retrieval policies.","authors":"Emma Donigan, Leigh Meyer, Kirsten A Riggan, Megan A Allyse","doi":"10.1007/s10815-025-03768-z","DOIUrl":"10.1007/s10815-025-03768-z","url":null,"abstract":"","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":"1007-1012"},"PeriodicalIF":2.7,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12982816/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145714446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hydrogel-enabled modulation of cryoinjury in oocyte vitrification: A pharmaceutical formulation perspective.","authors":"Yifei Yang, Wenjing Gong, Longfa Kou","doi":"10.1007/s10815-025-03797-8","DOIUrl":"10.1007/s10815-025-03797-8","url":null,"abstract":"","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":"1023-1024"},"PeriodicalIF":2.7,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12982709/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145998216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Donor sperm IVF pregnancies exhibit elevated risk of new-onset hypertensive disorders: a retrospective cohort study.","authors":"Binbin Tu, Mengjie Fan, Ping Liu, Rong Li, Jie Qiao, Rui Yang","doi":"10.1007/s10815-026-03834-0","DOIUrl":"10.1007/s10815-026-03834-0","url":null,"abstract":"<p><strong>Purpose: </strong>To evaluate the incidence of new-onset Hypertensive Disorders of Pregnancy (HDP) in donor sperm IVF (DS-IVF) compared with partner sperm IVF (PS-IVF) pregnancies.</p><p><strong>Methods: </strong>We retrospectively analyzed a total of 855 DS-IVF cycles and 4,816 PS-IVF cycles of pregnancies delivering after 20 weeks' gestation achieved live birth between January 2010 and December 2022. All patients underwent fresh embryo transfer after oocyte retrieval. The incidence of new-onset HDP was compared between the two groups.</p><p><strong>Results: </strong>The overall incidence of new-onset HDP was 1.7%, with rates of 2.7% in DS-IVF group and 1.6% in PS-IVF group. Multivariate Analysis of risk factors in the entire cohort identified twin pregnancy (OR, 2.483: 95%CI: 1.649-3.739, P < 0.001), elevated body mass index (BMI) (OR, 1.150: 95%CI: 1.095-1.209, P < 0.001), and the use of donor sperm (OR, 2.063: 95%CI: 1.229-3.463, P = 0.006) as independent risk factors for new-onset HDP. In subgroup analyses of both DS-IVF and PS-IVF cohorts, twin pregnancy [DS-IVF: OR, 2.575: 95%CI: 1.085-6.115, P = 0.032; PS-IVF: OR, 2.454: 95%CI: 1.542-3.906, p < 0.001] and elevated BMI [DS-IVF: OR, 1.152: 95%CI: 1.026-1.292, P = 0.016; PS-IVF: OR, 1.150: 95%CI: 1.089-1.215, p < 0.001] were identified as independent risk factors for new-onset HDP.</p><p><strong>Conclusion: </strong>The use of donor sperm is an independent risk factor for new-onset HDP in IVF-conceived pregnancies.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":"789-796"},"PeriodicalIF":2.7,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12982812/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147283932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The burden of terminology in IVF: refining efficiency metrics beyond \"time to pregnancy\".","authors":"Francesco Capodanno, Lucia De Santis, Laura Girardi, Enrica Capitanio, Alessia Galimberti, Laura Zanga, Sara Melis, Fernanda Maciel Madureira Lins, Antonella Di Pasqua, Francesco Maria Fusi","doi":"10.1007/s10815-026-03837-x","DOIUrl":"https://doi.org/10.1007/s10815-026-03837-x","url":null,"abstract":"","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147321689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nonlinear association of serum luteinizing hormone on hCG trigger day with cumulative live birth in IVF cycles: secondary analysis of a randomized clinical trial.","authors":"Ling Guo, Binbin Zhao, Zirui Peng, Keke Wei, Zi-Jiang Chen, Junhao Yan, Yan Li, Xinbo Xu, Nan Lu, Yaxin Su","doi":"10.1007/s10815-025-03773-2","DOIUrl":"10.1007/s10815-025-03773-2","url":null,"abstract":"<p><strong>Purpose: </strong>To investigate the impact of serum luteinizing hormone (LH) levels on the day of human chorionic gonadotropin (hCG) trigger on cumulative live birth in women undergoing in vitro fertilization (IVF) cycles.</p><p><strong>Methods: </strong>This was a secondary analysis of a multicenter randomized controlled trial (NCT03118141). A total of 1212 infertile women undergoing their first IVF cycle with a good prognosis for live birth were included. Participants were stratified into three groups according to tertiles of serum LH levels on the hCG trigger day (hLH): low-hLH (< 1.28 IU/L), medium-hLH (1.28-3.26 IU/L), and high-hLH (> 3.26 IU/L). Logistic regression and restricted cubic spline models were used to evaluate associations, adjusting for maternal age, body mass index, and baseline hormones.</p><p><strong>Results: </strong>Compared to the medium-hLH group, the low-hLH group exhibited significantly lower cumulative clinical pregnancy and live birth rates, along with a higher biochemical pregnancy loss rate (P < 0.05). The high-hLH group demonstrated a reduced cumulative live birth rate, while other pregnancy outcomes were comparable to the medium-hLH group. A restricted cubic spline analysis revealed a significant nonlinear, inverted U-shaped relationship between serum hLH levels and cumulative live birth rates, with the optimal threshold around 1.95 IU/L. Stratified analysis indicated that the impact of hLH levels was more pronounced in women undergoing GnRH antagonist protocols.</p><p><strong>Conclusions: </strong>Serum hLH levels exhibit a nonlinear association with cumulative live birth rates in IVF cycles, highlighting 1.95 IU/L as a potential threshold. Individualized adjustment of hLH levels may improve pregnancy outcomes, especially in GnRH antagonist protocols.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":"759-769"},"PeriodicalIF":2.7,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12982701/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145762799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}