Journal of Assisted Reproduction and Genetics最新文献

{"title":"Two sides of the same algorithm: a qualitative study of ART professionals' and patients' views on the use of machine learning for embryo assessment and selection.","authors":"Amy N S Webb, Andrea Whittaker, Julian J Koplin, Cal Volks, Catherine Mills, Molly Johnston","doi":"10.1007/s10815-026-03932-z","DOIUrl":"10.1007/s10815-026-03932-z","url":null,"abstract":"<p><strong>Purpose: </strong>Machine learning (ML) is increasingly being introduced into assisted reproduction clinical practice, particularly for embryo grading and selection. This study aimed to explore how assisted reproductive technology (ART) professionals, regulators and patients understand the use of ML in embryo assessment.</p><p><strong>Methods: </strong>Semi-structured interviews were conducted with ten ART professionals/regulators and ten patients undertaking assisted reproduction in Australian clinics. Interviews explored themes identified as ethically salient to clinical implementation.</p><p><strong>Results: </strong>Both professionals and patients expressed mistrust of ML and emphasised the importance of human oversight in embryo selection. Participants identified uncertainties regarding how ML produces knowledge and raised questions about accountability and responsibility for ML-assisted decisions. Concerns were expressed that ML may lead to the discarding of viable embryos in the pursuit of selecting embryos. Participants called for full disclosure from clinics regarding the use of ML. Most professionals expressed a preference for transparent ML models and raised concerns about incorrect scoring due to poorly trained algorithms, handling errors, and potential algorithmic bias. Some professionals also expressed concern about over-reliance on ML and the potential for workforce deskilling.</p><p><strong>Conclusion: </strong>ART professionals and patients are cautious about the introduction of ML into embryo selection and emphasize the continued importance of human oversight, transparency, and accountability. Knowledge production with ML remains contested and there is uncertainty on how best to incorporate ML and reconfigure the work of embryologists and their professional expertise.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":"2391-2398"},"PeriodicalIF":2.8,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13598045/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148264747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Concordance, discordance, and conflation: mired in the admixture of ARTs and Add-ons.","authors":"David F Albertini","doi":"10.1007/s10815-026-04031-9","DOIUrl":"10.1007/s10815-026-04031-9","url":null,"abstract":"","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":"2303-2304"},"PeriodicalIF":2.8,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13598037/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148944651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel HPLC-laser-stimulated fluorescence profiling of seminal plasma amino acids linked to ejaculate quality.","authors":"Megha Naik, Huidrom Yaiphaba Meitei, Shubhashree Uppangala, Dhakshanya Predheepan, Satish Kumar Adiga, Ajeetkumar Patil","doi":"10.1007/s10815-026-03944-9","DOIUrl":"10.1007/s10815-026-03944-9","url":null,"abstract":"<p><strong>Purpose: </strong>Amino acids are vital biomolecules that can provide important insights into sperm functional competence. However, the level of amino acids (AAs) in seminal plasma and their implications on sperm characteristics remain less understood.</p><p><strong>Methods: </strong>For the first time, high-performance liquid chromatography (HPLC) in combination with a laser-stimulated fluorescence (LSF) system was used to profile AAs from the seminal plasma of 30 normozoospermic and 51 nonnormozoospermic ejaculates. Sperm characteristics, along with DNA integrity, were assessed and correlated with individual AAs.</p><p><strong>Results: </strong>The HPLC-LSF system was able to detect a total of 18 AAs in the seminal plasma. A significant variation was observed between the two groups for all AAs (p < 0.05), except for aspartate. Notably, the relative intensities of AAs were significantly lower in the non-normozoospermic group. Principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) further highlighted distinct clusters based on the AA profiles of the two groups. The pathway analysis indicated differences in the metabolic pathways between the normozoospermic and nonnormozoospermic groups.</p><p><strong>Conclusion: </strong>The HPLC-LSF platform could serve as a potential tool in identifying seminal plasma AA levels. The low relative abundance of AA in the non-normozoospermic men could be attributed to aberrant metabolic pathways, underscoring their significance in male infertility.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":"2543-2555"},"PeriodicalIF":2.8,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13598000/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148368030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of embryo transfer catheter maneuvers on clinical pregnancy and live birth.","authors":"Jeremy Applebaum, Sophia Yin, Elizabeth Ginsburg, Andrea Lanes","doi":"10.1007/s10815-026-03945-8","DOIUrl":"10.1007/s10815-026-03945-8","url":null,"abstract":"<p><strong>Purpose: </strong>To investigate the association between embryo transfer (ET) catheter maneuvers on clinical pregnancy (CP) and live birth (LB).</p><p><strong>Methods: </strong>This retrospective cohort study included all in vitro fertilization and intracytoplasmic sperm injection cycles resulting in fresh or frozen ET at a single academic institution from 2013 to 2024. Catheter maneuvers were categorized as non-afterload or afterload. Non-afterload techniques included direct (straight outer sheath and inner catheter inserted together without manipulation), outer sheath curved, outer sheath extended, and outer sheath both curved and extended. Afterload techniques included: straight afterload (outer sheath retained, inner catheter removed, embryo loaded through another inner catheter), and curved afterload (curved outer sheath retained, inner catheter removed, and embryo loaded through another inner catheter). Adjusted log binomial regression assessed associations between ET catheter maneuvers and CP and LB.</p><p><strong>Results: </strong>Among 7882 ETs, 46.0% were direct, 1.9% curved, 8.8% extended, 10.2% curved and extended, 6.7% straight afterload, and 26.5% curved afterload. Afterload ETs were associated with lower adjusted relative risk (aRR) of CP (aRR 0.91, 95% confidence interval [CI] 0.85-0.98) and LB (aRR 0.88, 95% CI 0.81-0.96) compared to a direct technique. Curved afterload ETs had a lower aRR of both CP (aRR 0.91, 95% CI 0.85-0.98) and LB (aRR 0.92, 95% CI 0.86-0.99). Straight afterload ETs were associated with lower LB (aRR 0.89, 95% CI 0.81-0.99), but not CP (aRR 0.93, 95% CI 0.85-1.02). Non-afterload variations were not associated with outcome differences.</p><p><strong>Conclusion: </strong>ET afterload techniques are associated with lower CP and LB rates compared to a direct technique.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":"2485-2491"},"PeriodicalIF":2.8,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13598001/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148295163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ubiquitinated proteomics reveals potential epigenetic-energy metabolism mechanisms in senescent ovarian granulosa cells of advanced maternal age.","authors":"Xinxin Yang, Zhicheng Jia, Mengyu Shi, Xinwei Sun, Wenlong Qi, Ying Guo","doi":"10.1007/s10815-026-03926-x","DOIUrl":"10.1007/s10815-026-03926-x","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Purpose: &lt;/strong&gt;Ubiquitination represents a promising therapeutic target for age-associated diseases, yet its functional role in ovarian aging remains poorly defined. This study systematically characterizes ubiquitinated proteins in luteinized granulosa cells (GCs) from women of advanced maternal age (AMA), aiming to provide molecular insights into the ubiquitination-dependent regulatory network underlying ovarian aging and further elucidate the mechanisms governing energy metabolism reprogramming in senescent GCs.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;A total of 60 patients undergoing their first in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) were recruited in this study, including 30 patients with young maternal age (YMA) and normal ovarian reserve, and 30 patients with advanced maternal age (AMA) and ovarian aging. The recruitment period was from November 2024 to May 2025. Nine samples of luteinized GCs were randomly selected from each group for ubiquitinated proteomic sequencing analysis. Functional exploration of differentially ubiquitinated proteins was performed via Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses. Protein-protein interaction (PPI) network analysis was conducted to identify molecular targets of potential pathways. Additionally, the ubiquitination level and protein expression of alpha-enolase (ENO1) were validated by immunoprecipitation Western blot (IP-WB) and Western blot (WB) assays, respectively. Furthermore, an in vitro oxidative stress-induced ovarian aging model was established using hydrogen peroxide (H₂O₂)-treated human granulosa-like tumor cells line (KGN cells) to further investigate the regulatory role of ENO1 in energy metabolism reprogramming of GCs during ovarian aging.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;A total of 174 ubiquitinated peptides with significant differences were screened between the two groups (|log₂ FC|&gt; 1, q &lt; 0.05). Compared with the YMA group, 80 ubiquitinated peptides were significantly upregulated and 94 were significantly downregulated in the AMA group. GO enrichment analysis revealed that differentially ubiquitinated proteins (DUPs) were enriched in the \"metabolic pathways\" of biological processes (P &lt; 0.05). Further KEGG pathway analysis suggested that the \"ubiquitin-mediated protein degradation\" and \"proteasome\" pathways may be involved in the regulation of ovarian aging. In addition, DUPs exhibited differential ubiquitination characteristics in energy metabolism pathways: the ubiquitination levels of proteins related to the \"tricarboxylic acid (TCA) cycle\" pathway were upregulated, while those related to the \"glycolysis\" and \"pentose phosphate pathway (PPP)\" were downregulated (P &lt; 0.05). PPI network analysis indicated that ENO1, a differentially ubiquitinated protein (FDR &lt; 0.05) and a key glycolytic enzyme, may serve as a critical target for energy metabolism reprogramming during ovarian aging. WB and IP-WB results show","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":"2619-2639"},"PeriodicalIF":2.8,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13598013/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148345609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of 46,XX/46,XY chimerism in a normal fertile male by low-pass copy number variation sequencing and NGS-based STR tests.","authors":"Yiming Sun, Lulu Xiang, Caixia Lei, Tao Li, Zhichao Li, Yijuan Sun, Xiaoxi Sun","doi":"10.1007/s10815-026-03938-7","DOIUrl":"10.1007/s10815-026-03938-7","url":null,"abstract":"<p><strong>Purpose: </strong>To describe a rare case of a normal fertile male with the specific tissue distribution of 46,XX in the peripheral blood and gonadal chimerism confirmed by the novel next-generation sequencing (NGS) methodology.</p><p><strong>Methods: </strong>Cytogenetic analyses, low-pass copy number variation sequencing (CNV-seq) and NGS-based short tandem repeat (STR) tests.</p><p><strong>Results: </strong>Routine peripheral blood karyotyping showed 46,XX. Fluorescence in situ hybridization (FISH) analysis of lymphocyte metaphase nuclei confirmed 46,XX and sex-determine region Y (SRY)-negative. Further CNV-seq revealed the presence of Y chromosome in the semen. NGS-based STR tests discovered low-level Y chromosome in the buccal mucosa and three alleles in the semen, suggesting that this chimerism is likely the outcome of a parthenogenetically activated oocyte fertilized by a Y and X sperm. The patient underwent intracytoplasmic sperm injection (ICSI) and resulted in a normal singleton pregnancy.</p><p><strong>Conclusion: </strong>Individuals with 46,XX/46,XY chimerism in the gonad can display normal phenotype and fertility. NGS has proved to be a promising alternative to conventional chimerism assays.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":"2659-2666"},"PeriodicalIF":2.8,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13598014/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148308520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Platelet-rich plasma hydrogel supports long-term germ cell maintenance in rat testicular organotypic culture.","authors":"Nilgün Öksel, Merve Bulut Özer, İlayda Yücel, Muzaffer Katar","doi":"10.1007/s10815-026-03936-9","DOIUrl":"10.1007/s10815-026-03936-9","url":null,"abstract":"<p><strong>Purpose: </strong>Fertility preservation has become an important clinical concern for prepubertal boys undergoing gonadotoxic cancer treatments. Preservation of germ cells within immature testicular tissue is therefore considered a promising strategy for future fertility restoration. The purpose of this study was to evaluate the effects of platelet-rich plasma (PRP), used as a culture medium supplement and/or as a hydrogel scaffold, on germ cell survival and spermatogenic progression in a three-dimensional rat testicular organotypic culture system and to explore its potential relevance for fertility preservation approaches.</p><p><strong>Methods: </strong>Testicular tissue fragments obtained from postnatal day 7-9 Wistar albino rats were cultured using an air-liquid interface organotypic culture system on agarose blocks. PRP was incorporated either as a hydrogel scaffold supporting the tissue fragments or as a soluble supplement in the culture medium. Cultures were maintained for 8 weeks, and samples were collected at weeks 1, 3, 4, 6, and 8. Spermatogenic progression was evaluated using histomorphometric and immunohistochemical analyses.</p><p><strong>Results: </strong>SCP3( +) spermatocytes were better preserved in PRP hydrogel-supported cultures, with persistence observed up to week 8 only in this group. In addition, PLZF( +) undifferentiated spermatogonia remained detectable at week 8 in PRP hydrogel cultures, indicating improved preservation of the spermatogonial population. In vitro spermatogenesis progressed up to the spermatocyte stage in all experimental conditions, whereas differentiation into round spermatids was not observed.</p><p><strong>Conclusions: </strong>This organotypic culture approach may support germ cell maintenance during long-term culture and may contribute to the development of future fertility preservation strategies.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":"2593-2604"},"PeriodicalIF":2.8,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13598019/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148308523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"\"Extended ovarian stimulation\": a prolonged ovarian exposure to gonadotrophins leads to an increase in the oocyte yield-evidence from the DuoStim experience.","authors":"Antonio La Marca, Martina Capuzzo","doi":"10.1007/s10815-026-03922-1","DOIUrl":"10.1007/s10815-026-03922-1","url":null,"abstract":"<p><p>The recent better understanding of folliculogenesis throughout the ovarian cycle (the \"continuous/waves\" theory), as well as the improvement of embryo survival rates after vitrification (which permits untangling the timing of embryo transfer from ovarian stimulation and oocyte yield) has permitted to elevate ovarian stimulation from the old classic single stimulation starting in the early follicular phase to novel strategies of ovarian stimulation, such as random start, luteal phase, and double ovarian stimulation. The role of FSH in follicular recruitment and growth is well known and is the cornerstone of ovarian stimulation. Prolonged exposure to FSH, as it happens in the double stimulation protocol, may lead to a potential increase in the number of oocytes obtainable after the second stimulation. A very high number of studies on DuoStim showed a greater number of oocytes retrieved, expressed as cumulus-oocyte complexes (COCs) or mature oocytes (MIIs), in the second ovarian stimulation rather than the first ovarian stimulation. This was particularly significant when a GnRH agonist was used to trigger the ovulation. Different hypotheses can be formulated to explain this eventuality including increased synchronization of antral follicles at the time of the second stimulation, enhanced granulosa cell sensitivity to FSH, the flare-up effect of the GnRH agonist at the time of the first triggering, and an increased rate of progression of small follicles to the antral recruitable stage as a consequence of the prolonged exposure to gonadotropins. The evidence that repeated back-to-back ovarian stimulations could lead to a progressive increase in the number of oocytes retrieved reported in our review opens the way to the consideration of innovative stimulation protocols based on this new concept of \"Extended ovarian stimulation.\" If future prospective studies designed on purpose will confirm our thesis that the prolonged FSH stimulation would involve a clear gain in the number of oocytes retrieved, this strategy could become the first therapeutic choice when the goal is to maximize the oocyte yield.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":"2341-2349"},"PeriodicalIF":2.8,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13597988/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148217999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The impact of prior hormonal intrauterine device (IUD) use on endometrial lining thickness in the fertility clinic setting: a retrospective cohort study.","authors":"Esther Chung, Fiona Wang, Jiaqi Zhang, Michael Strug, Lusine Aghajanova, Ruth Lathi","doi":"10.1007/s10815-026-03950-x","DOIUrl":"10.1007/s10815-026-03950-x","url":null,"abstract":"<p><strong>Purpose: </strong>Despite wide-spread use of intrauterine devices (IUDs), research on an IUD's long-term impact on endometrial growth and function is limited. Endometrial atrophy is a known mechanistic action of hormonal IUDs, and characterizing its reversibility is critical. Our study compared endometrial lining thickness (EMT) in patients undergoing fertility treatment with and without prior hormonal IUD use.</p><p><strong>Methods: </strong>This was a retrospective cohort study of 163 patients aged 18-45, each with at least 1 treatment cycle with ultrasound monitoring at a single academic institution between 2018 and 2023. Patients with prior hormonal IUD use (n = 72) were compared with patients without prior IUD exposure (n = 91). Primary outcomes included mid-cycle endometrial thickness (EMT) in the first monitored cycle, thin lining (< 7 mm), and persistently thin lining (< 7 mm in ≥ 2 cycles). Secondary outcomes included trilaminar endometrial pattern. Multivariable regression models adjusted for age, BMI, parity, infertility diagnosis, uterine pathology, history of uterine procedures, miscarriage history, and treatment protocol type. Exploratory analyses evaluated duration of hormonal IUD exposure.</p><p><strong>Results: </strong>Median EMT in the first monitored cycle was significantly lower among patients with prior hormonal IUD exposure compared with patients without prior IUD exposure (6.70 mm vs 8.60 mm, p < 0.001). Thin endometrium in the first cycle occurred in 63% of HORMONAL IUD users versus 16% of controls (RR 3.79, 95% CI 2.31-6.23, p < 0.001). Among patients with multiple cycles, persistently thin lining occurred in 64% of hormonal IUD users versus 10% of controls (RR 6.32, 95% CI 3.05-13.13, p < 0.001). In multivariable analyses, prior hormonal IUD exposure remained associated with thinner EMT in the first cycle (adjusted mean difference - 1.9 mm, 95% CI - 2.6 to - 1.2, p < 0.01) and increased risk of thin lining (adjusted RR 4.09, p < 0.01). Cycle-level analyses similarly demonstrated thinner endometrium and higher rates of thin lining among hormonal IUD users after adjustment for treatment protocol type. Longer duration of hormonal IUD use was not significantly associated with greater risk of thin lining.</p><p><strong>Conclusions: </strong>Prior hormonal IUD use was significantly associated with thinner EMT in patients undergoing fertility treatment. While thinner lining after IUD use is concerning in this specific population, future prospective studies are needed to evaluate the clinically relevant outcomes of live birth.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":"2473-2483"},"PeriodicalIF":2.8,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13598057/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148317265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Embryonic mosaicism in severe male factor infertility is unrelated to sperm retrieval technique.","authors":"Tamar Alkon, Carlos Hernandez-Nieto, Joseph Lee, Morgan Baird, Natan Bar-Chama, Martha Luna, Alan Copperman, Erkan Buyuk","doi":"10.1007/s10815-026-03968-1","DOIUrl":"10.1007/s10815-026-03968-1","url":null,"abstract":"<p><strong>Purpose: </strong>To evaluate the association between the method of sperm retrieval (ejaculated versus testicular sperm) and embryonic mosaicism in infertile couples with severe male factor infertility undergoing in vitro fertilization (IVF) with preimplantation genetic testing for aneuploidy (PGT-A).</p><p><strong>Methods: </strong>This retrospective study analyzed 298 patients with severe male factor infertility undergoing IVF with PGT-A from 2019 to 2025. Patients were divided into two groups based on sperm source: ejaculated sperm (n = 229) and testicular sperm obtained via testicular sperm extraction (TESE) (n = 69). All cycles used fresh sperm collected on the day of oocyte retrieval. The primary outcome was the rate of embryonic mosaicism among biopsied blastocysts. Secondary outcomes included euploidy, aneuploidy, mosaicism level, inconclusive embryo count, clinical pregnancy and live birth rates.</p><p><strong>Results: </strong>A total of 1124 embryos were biopsied (250 from testicular sperm extraction; 874 from ejaculate). Mosaicism rates were similar between testicular and ejaculated sperm groups (9.6% vs. 9.4%, p = 0.62). Sperm retrieval method was not associated with mosaicism (OR 0.98, CI 0.61-1.58), euploidy (OR 0.90, CI 0.70-1.08), or aneuploidy (OR 0.89, CI 0.60-1.06). High- and low-level mosaicism were also comparable between groups (OR 0.96, CI 0.75-1.18 and OR 0.99, CI 0.81-1.22). No differences were observed in clinical pregnancy and live birth rates among cohorts.</p><p><strong>Conclusions: </strong>Sperm retrieval technique does not influence embryonic mosaicism rates in patients with severe male factor infertility. These findings support patient counseling by indicating that invasive procedures should not be pursued solely for the purpose of reducing mosaicism rates.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":"2515-2523"},"PeriodicalIF":2.8,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13598039/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148421371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}