Journal of Assisted Reproduction and Genetics最新文献

{"title":"Impact of COVID-19 on live birth and perinatal outcomes following subsequent frozen-thawed embryo transfer.","authors":"Yao Lu, Zhe Wei, Yaqiong He, Jiaan Huang, Qinling Zhu, Ying Ding, Wen Lin, Steven R Lindheim, Yun Sun","doi":"10.1007/s10815-025-03573-8","DOIUrl":"https://doi.org/10.1007/s10815-025-03573-8","url":null,"abstract":"<p><strong>Purpose: </strong>To evaluate whether prior SARS-CoV-2 infection adversely affects live birth and perinatal outcomes following subsequent frozen-thawed embryo transfer (FET) and to further investigate whether the time interval between infection and embryo transfer influences pregnancy outcomes.</p><p><strong>Methods: </strong>In this retrospective cohort study, women who tested positive for SARS-CoV-2 before FET were designated as the infected group (n = 1081), while asymptomatic and test-negative women were included as controls (n = 865). The infected group was further subdivided into four subgroups according to the time interval from infection to FET: ≤ 30, 31-60, 61-90, and ≥ 91 days.</p><p><strong>Results: </strong>Overall, positive HCG rate was significantly lower in the infected group compared to the non-infected group (62.9% vs. 67.5%, adjusted OR = 0.80, 95% CI = 0.66-0.97, P = 0.021), while no significant differences were observed in live birth rates (45.9% vs. 49.7%, adjusted OR = 0.84, 95% CI = 0.70-1.01, P = 0.062). There were no significant differences in clinical pregnancy, pregnancy loss, or any of the measured obstetric and neonatal outcomes between groups. However, women in the ≤ 30 days' subgroup were associated with a significant reduction in positive HCG (adjusted OR = 0.52, 95% CI = 0.33-0.84, P = 0.007), clinical pregnancy (adjusted OR = 0.60, 95% CI = 0.38-0.95, P = 0.029), and live birth rates (adjusted OR = 0.51, 95% CI = 0.31-0.83, P = 0.006) compared to those in the non-infected group, while no such association was observed with the other subgroups.</p><p><strong>Conclusions: </strong>In those undergoing FET, overall, prior SARS-CoV-2 infection does not adversely affect live birth and perinatal outcomes. However, considering the decreased likelihood of live birth among women with shorter intervals, delaying the subsequent FET for at least 30 days after infection could be beneficial.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144855310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of missense DMC1 variants in males with non-obstructive azoospermia.","authors":"Noor Ullah, Christopher Pombar, Rachel Hvasta-Gloria, Andrea J Berman, Michelle Malizio, Muhammad Jaseem Khan, Rubina Nazli, Sadia Fatima, Antoni Riera-Escamilla, Helen Castillo-Madeen, Agnieszka Malcher, Marta Olszewska, Miguel J Xavier, Kristiina Lillepea, Avirup Dutta, Carlos A Castro, Anu Valkna, Rain Inno, Kyle E Orwig, Donald F Conrad, Maciej Kurpisz, Joris A Veltman, Maris Laan, Alexander N Yatsenko","doi":"10.1007/s10815-025-03591-6","DOIUrl":"10.1007/s10815-025-03591-6","url":null,"abstract":"<p><strong>Purpose: </strong>Human male infertility is a significant reproductive condition, with non-obstructive azoospermia (NOA) being the most severe form, resulting from impaired spermatogenesis. Many genetic variants have been identified as negatively impacting sperm development and maturation at multiple stages, leading to spermatogenic failure (SPGF). Here, we aim to study such variants, particularly those in the critical, highly conserved, meiosis-specific DMC1 (DNA meiotic recombinase 1) gene, to identify genetic candidates for male infertility and to strengthen DMC1's existing genotype-phenotype relationships.</p><p><strong>Methods: </strong>We used whole exome sequencing (WES) and in silico analysis to investigate select DMC1 variants in a large cohort of infertile sporadic and familial cases (n = 3150).</p><p><strong>Results: </strong>Our familial analyses identified a homozygous DMC1 missense variant, p.Thr55Ile, in two NOA-affected male siblings. We also report additional homozygous missense variants, p.Thr164Ala and p.Tyr194Cys, and one notable, rare single heterozygous variant, p.Asp160Gly, in unrelated sporadic patients. Our 3D protein modeling indicates that each of our identified variants would significantly impact the structure and functional activity of DMC1 protein.</p><p><strong>Conclusion: </strong>Our extensive genomic study identified three rare, recessive DMC1 variants in human NOA patients. Further, we report an alternative maturation arrest phenotype than previously observed in DMC1-related NOA. We also provide preliminary support for the possible exploration of select single heterozygous variants in the DMC1 gene, potentially expanding the male infertility field's understanding of the disease states and inheritance patterns associated with variants in DMC1.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144855309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Global, regional, and national burden and trend of infertility and its subtypes from 1990 to 2021, with projections to 2035.","authors":"Xi Cheng, Jinzhao Ma, Wenhua Wang, Xiting Cai, Biying Li, Li Chen, Bing Yao","doi":"10.1007/s10815-025-03592-5","DOIUrl":"https://doi.org/10.1007/s10815-025-03592-5","url":null,"abstract":"<p><strong>Purpose: </strong>To study the global, regional and national burden and trend of primary and secondary infertility, by sex and sociodemographic index (SDI), over the period 1990-2021.</p><p><strong>Methods: </strong>Data on the prevalence and disability-adjusted life years (DALYs) due to infertility and its subtypes were retrieved from the GBD study 2021 for 204 countries and territories from 1990 to 2021. The counts and age-standardized rates are presented, along with their corresponding 95% uncertainty intervals (UIs). Estimated annual percentage changes (EAPCs) were computed using linear regression analysis. Decomposition analysis was conducted to determine the effects of population growth, aging, and epidemiological shifts on disease burden. Frontier analysis was performed to identify potential improvement areas and disparities among countries by development status. Bayesian age-period-cohort analysis (BAPC) predicted global lung cancer mortality from 2020 to 2035.</p><p><strong>Results: </strong>Globally, the age-standardized prevalence rate (ASPR) of infertility changed from 4071.04 (95% UI: 2660.05, 6223.25) per 100,000 in 1990 to 5030.42 (3207.13, 7918.99) per 100,000 in 2021, with the EAPC being 0.65. The age-standardized DALYs rate (ASDR) changed from 23.15 (8.91, 53.73) per 100,000 in 1990 to 28.54 (11.05, 67.94) per 100,000 in 2021, with the EAPC being 0.52. It is anticipated that the ASPR exhibits a downward trend for overall primary infertility, whereas they are expected to show an upward trend for overall secondary infertility by 2035, in comparison with 2021.</p><p><strong>Conclusions: </strong>Governments and the public should recognize the magnitude of the issue of infertility and prioritize implementing targeted interventions and strategies to improve reproductive health worldwide.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144859252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating perceptions and usage of fertility supplements: a mixed methods analysis of a large online forum.","authors":"Ana F Tomlinson, Meghana Chapalamadugu, Aishwarya Hombal, Suset Rodriguez, Pasquale Patrizio","doi":"10.1007/s10815-025-03625-z","DOIUrl":"https://doi.org/10.1007/s10815-025-03625-z","url":null,"abstract":"<p><strong>Purpose: </strong>This study aims to characterize assisted reproductive technology patients' online discussions of fertility supplements to better understand how this audience uses supplements, their attitudes toward perceived effects of the supplement, and the topics patients sought advice on regarding supplements.</p><p><strong>Methods: </strong>This study used mixed methods, sequential exploratory design. We extracted public posts from the Reddit forum, \"r/IVF.\" Posts about fertility supplements were categorized by the described use of supplements and perceived effect of the supplement. Posts in each qualitative category were then quantified, and post author characteristics were analyzed using descriptive statistics.</p><p><strong>Results: </strong>Three hundred sixty-nine posts were included in the analysis. Two hundred nine posts identified specific supplements, and the most frequently mentioned included ubiquinone, vitamin D, omega-3 fatty acids, dehydroepiandrosterone, and myo-inositol. Two hundred seventy-nine authors reported taking supplements; 9.3% reported a positive perceived effect, 12.9% reported a negative perceived effect, and 21.1% asked for advice regarding their supplements. In the remaining 90 posts, 10% of authors expressed concerns and 90% expressed interest in taking supplements. One hundred ninety-seven posts included the indication for using assisted reproductive technology, the most common being diminished ovarian reserve or male factor infertility.</p><p><strong>Conclusion: </strong>Many patients are using supplements with the goal of increasing their chance of assisted reproductive technology success and are seeking guidance on their use online. Some frequently mentioned supplements have limited research and unknown efficacy. The extensive discourse about supplements observed in this study reflects a need for increased guidance and evidence-based medical advice on how patients may use supplements safely and appropriately.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144846639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between triglyceride-glucose index combinations and infertility: findings from a nationally representative study.","authors":"Zi-Qi Dai, Ming-Yue Kong, Tian-Jiao Li, Xu-Jin Wu, Xue-Song Zhang, Rui Li, Xin Chen","doi":"10.1007/s10815-025-03618-y","DOIUrl":"https://doi.org/10.1007/s10815-025-03618-y","url":null,"abstract":"<p><strong>Background: </strong>Infertility has emerged as a significant public health concern with implications for population development. Despite its relevance, there has been limited research addressing the association between infertility and triglyceride-glucose (TyG) index-derived indicators. This study examined the relationship between infertility and three TyG-related composite indices: TyG-BMI (TyG index combined with body mass index), TyG-WC (TyG index combined with waist circumference), and TyG-WHtR (TyG index combined with waist-to-height ratio).</p><p><strong>Methods: </strong>Data were obtained from the 2013-2020 National Health and Nutrition Examination Survey, including a representative sample of 2856 adult participants. TyG-related indices were calculated using fasting triglyceride and glucose concentrations in combination with anthropometric measurements. Associations between infertility and TyG, TyG-BMI, TyG-WC, and TyG-WHtR were assessed using weighted multivariate logistic regression, subgroup analyses, smooth curve fitting, and threshold effect modeling.</p><p><strong>Results: </strong>Significant positive associations were observed between infertility and TyG-BMI (odds ratio (OR), 1.003; 95% confidence interval (CI), 1.001-1.004), TyG-WC (OR, 1.001; 95% CI, 1.000-1.002), and TyG-WHtR (OR, 1.186; 95% CI, 1.058-1.329). Subgroup analyses indicated consistent positive associations within specific populations. Among Mexican American participants, all TyG-related indicators were significantly associated with infertility. In married participants and non-Hispanic White participants, TyG-BMI, TyG-WC, and TyG-WHtR demonstrated significant associations with infertility. Two-stage linear regression analyses did not depict any evidence of nonlinear relationships between these indices and infertility.</p><p><strong>Conclusion: </strong>TyG-BMI, TyG-WC, and TyG-WHtR were identified as risk factors for infertility, with no observed nonlinear associations. These findings highlight the relevance of TyG-related metabolic indicators in reproductive health, warranting further investigation into their underlying pathophysiological mechanisms.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144846638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlation of fetal heartbeat outcome after Day 3 or Day 5 single embryo transfer of morphologically selected embryos with an annotation-free deep learning scoring system: Results from a multi-center study.","authors":"Wei Han, Bo Huang, Jiahong Zhu, Jiayi Zou, Xia Xue, Yufei Yao, Lei Jin, Yanlin Ma, Juanzi Shi, Guoning Huang","doi":"10.1007/s10815-025-03570-x","DOIUrl":"https://doi.org/10.1007/s10815-025-03570-x","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate whether the use of a fully automated AI-based scoring system (iDAScore V2) for selecting viable embryos using fetal heartbeat (FHB) as an indicator is equivalent to morphology assessment.</p><p><strong>Methods: </strong>A retrospective observational cohort study across four fertility centers analyzed embryos selected for single embryo transfer on Day 3 or Day 5 + based on morphology and time-lapse video. All transferred embryos from participating centers were retrospectively scored using a fully automated AI-based embryo scoring algorithm and standardized morphology assessment. The predictive ability of both methods for implantation (FHB rate) was compared for Day 3 and Day 5 + transfer.</p><p><strong>Results: </strong>A multi-center analysis revealed that AI-based embryo scoring significantly outperformed morphological embryo assessment in predicting FHB for both Day 3 (n = 2965) and Day 5 + (n = 6970) transfers (P < 0.0001). Similarly, the discrimination of low versus high scores regarding FHB resulted in a significantly better area under the curve (AUC) for iDAScore V2 compared to standardized morphology assessment for Day 3 (0.63; 95% CI: 0.61-0.65 versus 0.59; 95% CI: 0.58-0.61) and for Day 5 + (0.59; 95% CI: 0.57-0.60 versus 0.55; 95% CI: 0.54-0.57).</p><p><strong>Conclusions: </strong>As a multi-center validation of fully automated embryo assessment, this study confirms that AI-based selection provides outcomes that are either equivalent to or superior to morphological embryo assessment, without compromising clinical outcomes.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144821552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of advanced degrees in reproductive endocrinology and infertility specialists.","authors":"Elisheva Knopf, Elana Eisenreich, Shalakha Bonthu, Pranav Meka, Leah Roberts, Michele Retrouvey","doi":"10.1007/s10815-025-03590-7","DOIUrl":"https://doi.org/10.1007/s10815-025-03590-7","url":null,"abstract":"<p><strong>Purpose: </strong>Many physicians pursue advanced degrees (ADs), defined as a master's or doctoral-level degree, in addition to a medical degree to enhance professional development. This study aimed to examine how holding an AD influences the career trajectory amongst reproductive endocrinology and infertility (REI) specialists.</p><p><strong>Methods: </strong>A list of REI physicians was obtained from the American Society of Reproductive Medicine database. Physicians were divided based on AD status and compared across demographics, academic leadership, journal board, and reproductive society officer positions, and research productivity, measured by h-index and number of publications and citations. Fisher's Exact T-tests, Chi-Squared analysis, and Mann-Whitney U tests were used for associations.</p><p><strong>Results: </strong>Amongst 768 REI physicians, 163 (21%) possessed an AD. Proportionally more female physicians held an AD compared to males (23.4% vs. 19.4%, p < 0.001). Those with ADs were more likely to have an allopathic medical degree (98.8%), practice in the Northeast of the USA (33.7%), and work in private practice (55.8%) (p < 0.001). REI physicians with ADs demonstrated higher research productivity, reflected by higher average h-index (18.33 vs. 11.96), publications number (58.8 vs. 36.25), and citations number (2524 vs. 1531) (p < 0.001). They were also more likely to hold academic leadership, journal board, or reproductive society officer positions (p < 0.001).</p><p><strong>Conclusions: </strong>Holding an AD was associated with greater research productivity and higher representation in academic leadership roles among REI physicians. These insights may help current and aspiring REIs evaluate the role of additional qualifications in achieving their professional goals.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144821553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Decrease or increase in sperm concentration? The data among fertile Chinese males in 40 years.","authors":"Haiying Geng, Elizabeth R Rayburn, Qiuhong Wang, Yunting Chen, Xuejun Shang, Haibo Li","doi":"10.1007/s10815-025-03563-w","DOIUrl":"https://doi.org/10.1007/s10815-025-03563-w","url":null,"abstract":"<p><strong>Purpose: </strong>This study aims to investigate the trends in sperm concentration among fertile Chinese males over the past four decades, addressing whether there has been a change in sperm concentration and identifying potential causes.</p><p><strong>Methods: </strong>The research included 94 Chinese and English literature sources, covering 27 provinces and cities across China, encompassing a total of 14,634 individuals. A least squares linear regression model was applied to analyze sperm concentration data.</p><p><strong>Results: </strong>The analysis indicated a significant downward trend overall from 1984 to 2023 (P = 8.42e - 10). A single-factor analysis of variance was performed, categorized by the year of sample collection, showing a decline in sperm concentration across each decade of the collection years (P = 1.01e - 08), with the most significant decline noted between 2004 and 2013 (P = 0.0035).</p><p><strong>Conclusion: </strong>The findings suggest a general decline in sperm concentration among fertile Chinese males over the past forty years, with a more pronounced decline after the twenty-first century, potentially associated with escalating environmental pollution due to heavy industrialization, poor lifestyle habits, and radiation from electronic devices. Further research is essential to elucidate the causes of and broader implications associated with this downward trend.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144816689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-oocyte follicles in the bovine ovary: occurrence, activation, and growth during early in vitro folliculogenesis in a dynamic culture bioreactor.","authors":"Andrea Candela, Vincenza De Gregorio, Vincenzo Genovese, Angela Travaglione, Mario Cimmino, Riccardo Talevi, Roberto Gualtieri","doi":"10.1007/s10815-025-03600-8","DOIUrl":"https://doi.org/10.1007/s10815-025-03600-8","url":null,"abstract":"<p><strong>Purpose: </strong>Multi-oocyte follicles (MOFs) have been identified in various mammals, including humans, yet their origin and function remain controversial. This study aimed to investigate the occurrence and early developmental dynamics of MOFs in bovine ovaries, comparing them to single-oocyte follicles (SOFs).</p><p><strong>Methods: </strong>Ovarian tissues from Bos taurus taurus individuals, including one case with an unusually high MOF incidence, were cultured in vitro under dynamic conditions. MOF frequency and their progression through early folliculogenesis stages were assessed histologically and via confocal microscopy.</p><p><strong>Results: </strong>MOFs were observed at varying frequencies, with one individual showing a notably high incidence (15.8%). In vitro culture confirmed that MOFs can activate and progress to secondary stages, similar to SOFs. However, differences in activation rates and oocyte number dynamics were noted between MOFs and SOFs, and between fresh and cultured tissues. No de novo formation of MOFs was detected in vitro.</p><p><strong>Conclusions: </strong>MOFs retain the capacity for early folliculogenesis comparable to SOFs, without increased atresia. Their stable frequency postculture supports a prenatal origin. These findings offer new insights into MOF biology and suggest a possible physiological relevance in mammalian reproductive systems.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144804192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spermatozoal sialidases NEU1 and NEU3 are potential predictors for fertilization rate in IVF: a prospective cohort study.","authors":"Xin Yuan, Jiajie Li, Tang Deng, Li Yang, Yilun Wu, Liangchen Xiang, Fang Yuan, Xinrui Sun, Yi Xia, Yihong Yang, Yi Zhang, Fang Ma, Yan Wang","doi":"10.1007/s10815-025-03602-6","DOIUrl":"https://doi.org/10.1007/s10815-025-03602-6","url":null,"abstract":"<p><strong>Purpose: </strong>This study aimed to explore the roles of spermatozoal sialidase NEU1 and NEU3 in affecting the fertilization rate of in vitro fertilization (IVF), and whether sperm sialidase NEU1 and NEU3 can be used as predictors of IVF outcome.</p><p><strong>Material and methods: </strong>This was a prospective cohort study that collected semen samples from 194 IVF couples between January 2024 and April 2024. Patients were grouped based on oocyte maturity and routine semen analysis. Detection of sperm NEU1 and NEU3 was carried out in semen from the same ejaculate for IVF by using flow cytometry, followed by correlation analysis with fertilization rates.</p><p><strong>Results: </strong>NEU1 and NEU3 are independent indicators separate from regular semen parameters. With a metaphase II (MII) rate ≥80% and normal semen routine analysis, sperm NEU1 (P = 0.0300) and NEU3 (P = 0.0253) levels were significantly different demarcated by 80% fertilization rate. Lower NEU1 and NEU3 expression on sperm is associated with lower fertilization rates. ROC curve analyses displaying the diagnostic accuracy of NEU1 (P = 0.0306, AUC = 0.660) and NEU3 (P = 0.0259, AUC = 0.665). χ² test analyzes the fertilization rate and the different cut-off rate for NEU1 (χ² = 4.671, P = 0.0307) or NEU3 (χ² = 6.377, P = 0.0116).</p><p><strong>Conclusions: </strong>NEU1 and NEU3 on sperm can predict the IVF fertilization outcome in the group with oocyte MII rate ≥80%. The protein levels of NEU1 and NEU3 on sperm could be a new index for assisted reproductive technology (ART).</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144799212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}