Journal of Assisted Reproduction and Genetics最新文献

{"title":"Impact of aging on spermatogenic function and reproductive outcomes in repro57 heterozygous male mice: A model for age-related infertility.","authors":"Yuto Aoki, Misaki Wakamatsu, Nanami Sono, Wei Xiao, Emi Ishii, Takeshi Nagai, Yasushi Nagai, Yasuhiro Fujiwara, Tetsuo Kunieda, Junko Otsuki","doi":"10.1007/s10815-025-03481-x","DOIUrl":"10.1007/s10815-025-03481-x","url":null,"abstract":"<p><strong>Purpose: </strong>This study aims to investigate the histological changes, sperm parameters, and their impact on embryo development rates and offspring numbers in advanced-age male repro57 heterozygous mice, corresponding to approximately 40 years of age in humans.</p><p><strong>Methods: </strong>Sperm parameters were assessed in both young and advanced-age repro57 heterozygous mice, as well as in young and advanced-age wild-type mice. Additionally, testis weight and histological analysis of seminiferous tubules were conducted to identify degenerative changes. Male mice from each group were mated with young wild-type females to compare offspring numbers, and in vitro fertilization (IVF) was used to evaluate fertilization and blastocyst formation rates.</p><p><strong>Results: </strong>No significant differences in sperm concentration and motility were observed between young and aged wild-type mice or between young wild-type and young repro57 heterozygous mice. However, advanced-age repro57 heterozygous mice exhibited significantly lower sperm parameters and testis weight compared to advanced-age wild-type mice. Histological analysis revealed increased Sertoli cell vacuolation in the seminiferous tubules of advanced-age repro57 heterozygous mice. Additionally, these advanced-age mice exhibited significantly lower blastocyst formation rates and produced fewer offspring compared to advanced-age wild-type mice.</p><p><strong>Conclusion: </strong>Advanced reproductive aging in repro57 heterozygous male mice is associated with marked senescence-like degenerative changes, leading to a decline in offspring numbers, attributed to increased Sertoli cell vacuolation and diminished sperm quality.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":"2019-2028"},"PeriodicalIF":3.2,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144015931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of novel variants and expansion of the phenotypic spectrum in PATL2, WEE2, and TUBB8 associated with human early embryonic arrest.","authors":"Haijing Zhao, Nengyong Ouyang, Songbang Ou, Haiyan Lin, Zaowen Liao, Wenyi Liu, Hui Chen, Ping Yuan","doi":"10.1007/s10815-025-03501-w","DOIUrl":"10.1007/s10815-025-03501-w","url":null,"abstract":"<p><strong>Purpose: </strong>This study aimed to identify the genetic variants associated with early embryonic developmental arrest (EDA) in infertile patients and to expand the genotypic and phenotypic spectrum of maternal-effect genes, including PATL2, WEE2, and TUBB8, which are critical for oocyte maturation arrest (OMA) and fertilization failure (FF) as previously reported.</p><p><strong>Methods: </strong>Whole-exome sequencing was performed on 84 unrelated patients who experienced multiple in vitro fertilization and embryo transfer failures due to EDA. The effects of the variants in arrested embryos were assessed by morphological observations. Variants in PATL2, WEE2, and TUBB8 were confirmed by Sanger sequencing, followed by bioinformatic analysis, structural modeling of proteins, and functional assays.</p><p><strong>Results: </strong>We identified seven variants in five patients, including five novel variants (PATL2: c.802G>C; WEE2: c.487T>A, c.1165_1168delAAAC; TUBB8: c.604A>T, c.848C>A) and two previously reported variants (PATL2: c.805C>A; TUBB8: c.322G>A). The variants were predicted to be deleterious, affecting amino acid residues that are highly conserved across species. In vitro experiments confirmed that the PATL2 missense mutation (p.Gln269Lys) resulted in elevated mRNA levels compared to the wild type in HEK293T cells, while the WEE2 variant (p.Tyr163Asn) showed a 20.97% reduction in enzymatic activity. The patients displayed a wide range of infertility phenotypes, including OMA, FF, cleavage failure, and EDA. A literature-based analysis further highlighted the broad and variable phenotype spectrum associated with variants in these genes, enhancing our understanding of genotype-phenotype correlations.</p><p><strong>Conclusions: </strong>This study highlights the diverse phenotypic outcomes associated with variants in PATL2, WEE2, and TUBB8. The findings provide a clearer picture of the genetic and phenotypic spectrums in patients, contributing to the advancement of molecular diagnostics in infertility.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":"1961-1973"},"PeriodicalIF":3.2,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144119346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pharmacogenetic analysis using artificial intelligence (AI) to identify polymorphisms associated with sub-optimal ovarian response and hyper-response.","authors":"José A Ortiz, B Lledó, L Luque, R Morales, S Myles, María Pérez, J Guerrero, A Bernabeu","doi":"10.1007/s10815-025-03471-z","DOIUrl":"10.1007/s10815-025-03471-z","url":null,"abstract":"<p><strong>Purpose: </strong>To identify genetic variants associated with an increased likelihood of sub-optimal ovarian response or hyper-response by machine learning.</p><p><strong>Methods: </strong>This retrospective observational study, conducted between March 2018 and April 2022, analyses 495 ovarian stimulations in oocyte donors. Only each donor's first ovarian stimulation was considered. The egg donors were healthy women aged 18 to 35 years. Donor characteristics and ovarian stimulation data were recorded, as well as genotypes of 31 polymorphisms previously identified as modulators of ovarian response. Models to predict the type of ovarian response (sub-optimal, normal, or hyper-response) were performed using 5 different classification machine-learning algorithms. The most important variables were determined by SHAP (Shapley-Additive-exPlanations) values.</p><p><strong>Results: </strong>Despite being young with good ovarian reserves and using similar stimulation protocols, 15.15% of oocyte donors had a sub-optimal response (4-9 oocytes), while 27.27% showed a hyper-response (over 20 oocytes). The best predictive model was random forest, with an AUC of 0.822. Six significant genetic polymorphisms were identified: three in hormone receptors-oestrogen receptor (ESR2; c.*39G > A, c.984G > A), follicle-stimulating hormone receptor (FSHR; p.Asn680Ser, c.-29G > A), and AMH receptor (AMHR2; c.622-6C > T) and one in growth differentiation factor 9 (GDF9; c.398-39G > C). Four polymorphisms (ESR2, FSHR) were linked to sub-optimal response, while two (AMHR2, GDF9) were associated with hyper-response.</p><p><strong>Conclusions: </strong>By using a predictive model to asses ovarian response, we identified six genetic polymorphisms associated with ovarian response. Women who carry these genetic variants may be suitable candidates for personalised ovarian stimulation treatments to help prevent inadequate responses.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":"1863-1873"},"PeriodicalIF":3.2,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143764077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quality of life, spiritual needs, and well-being of people affected by infertility and its treatment: quantitative results of a mixed-methods study.","authors":"Madeleine Bernet, Alexander M Quaas, Michael von Wolff, Alessandro Santi, Isabelle Streuli, Dorothea Wunder, Eva Soom Ammann, Arndt Büssing","doi":"10.1007/s10815-025-03463-z","DOIUrl":"10.1007/s10815-025-03463-z","url":null,"abstract":"<p><strong>Purpose: </strong>Infertility is a growing global health issue that significantly affects quality of life (QoL). Understanding its impact on QoL is essential for developing effective healthcare interventions. This study explored QoL, well-being, and spiritual needs among individuals affected by infertility, with implications for healthcare provision.</p><p><strong>Methods: </strong>Standardized questionnaire data from an anonymous mixed-methods study that was conducted from October 2022 to January 2023 in Switzerland and addressing QoL (FertiQoL), spiritual needs (SpNQ-20), and psychological well-being (WHO-5) of individuals undergoing fertility treatments.</p><p><strong>Results: </strong>The analysis included 326 participants. FertiQoL scores were lowest in the emotional domain (M = 46.35) and highest in the relational domain (M = 68.51), with a mean overall score of 56.69, indicating moderate QoL impacts. Participants without children reported significantly lower FertiQoL and WHO-5 well-being scores than those with children (p < 0.05). The WHO-5 mean score indicated moderate well-being (M = 13.89). SpNQ-20 results showed the highest needs in inner peace (M = 1.82), followed by generativity (M = 1.09) and existential needs (M = 0.86), with religious needs scoring the lowest (M = 0.43). Participants without children had significantly higher spiritual needs (p < 0.05).</p><p><strong>Conclusion: </strong>This study highlights the emotional and spiritual challenges of infertility, revealing differences in experiences between individuals with and without children. It emphasizes the importance of addressing mental health and well-being in infertility care. Further research should focus on the psychological impacts of fertility treatments, including depressive mood states.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":"1853-1862"},"PeriodicalIF":3.2,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143752956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diverse developmental velocities of blastocyst development emerge as early as a few hours after insemination: morphokinetic analysis of fertilisation in days 4-7 blastocysts.","authors":"Nanoha Fujiwara, Kenji Ezoe, Giovanni Coticchio, Danilo Cimadomo, Laura Rienzi, Keiichi Kato","doi":"10.1007/s10815-025-03476-8","DOIUrl":"10.1007/s10815-025-03476-8","url":null,"abstract":"<p><strong>Purpose: </strong>The time at which embryo developmental velocity variations occur remains unknown. Therefore, we analysed the morphokinetic trajectory of human early fertilisation from the extrusion of the second polar body (PB2) to pronuclear breakdown (PNBD) and retrospectively compared its alterations during the fertilisation of day 4-7 blastocysts.</p><p><strong>Methods: </strong>Patients (n = 905) who underwent oocyte retrieval and blastocyst expansion between October 2019 and December 2020 were included. This study involved time-lapse technology monitoring of day 4 (n = 29), 5 (n = 1181), 6 (n = 244), and 7 (n = 12) blastocysts generated over 1009 intracytoplasmic sperm injection cycles. Morphokinetics during fertilisation were compared among the day 4-7 blastocyst groups.</p><p><strong>Results: </strong>The timing of fertilisation events in the cytoplasm and pronuclei (PN) differed among the day 4-7 blastocyst groups from the outset. PB2 extrusion occurred significantly earlier in day 4 blastocysts than in day 5 blastocysts. The duration of the cytoplasmic halo was shortened in day 4 blastocysts and prolonged in day 6 blastocysts compared with that in day 5 blastocysts. Paternal PN appeared earlier in day 4 blastocysts than in day 5 blastocysts, whereas their appearance was delayed in day 6 blastocysts. In paternal and maternal PN, the time interval from PN appearance to PNBD was shortened in day 4 blastocysts but was prolonged in day 6 and 7 blastocysts.</p><p><strong>Conclusions: </strong>Embryos that reached the blastocyst stage between days 4 and 7 displayed different morphokinetic speeds throughout fertilisation and as early as PB2 extrusion. Aberrant cytoplasmic granules, organelles, and PN rearrangement may contribute to these differences.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":"1781-1793"},"PeriodicalIF":3.2,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144002349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Letrozole-stimulated cycles versus hormone replacement treatment cycles for frozen embryo transfer in women with polycystic ovary syndrome: a prospective randomized controlled trial.","authors":"Linlin Jiang, Jia Huang, Yanxin Xie, Lin Li, Ping Pan, Ruiyi Zhu, Dongzi Yang, Yu Li","doi":"10.1007/s10815-025-03500-x","DOIUrl":"10.1007/s10815-025-03500-x","url":null,"abstract":"<p><strong>Purpose: </strong>This study aimed to compare the pregnancy outcomes of letrozole (LE)-induced ovulation and hormone replacement treatment (HRT) in endometrial preparation for frozen embryo transfer (FET) in women with polycystic ovary syndrome (PCOS).</p><p><strong>Methods: </strong>A randomized controlled trial involved 200 patients with PCOS from December 2017 to December 2022. Participants, who underwent FET with one or two good-quality blastocysts or cleavage-stage embryos, were randomly assigned to the LE group or HRT group in a 1:1 ratio. The primary outcome was the clinical pregnancy rate. Secondary outcomes included biochemical pregnancy, implantation, ectopic pregnancy, miscarriage, multiple pregnancy, and live birth rates.</p><p><strong>Results: </strong>The clinical pregnancy rate was 66.0% in the LE group compared to 53.0% in the HRT group (absolute difference, 13.0% [95% CI, - 0.5 to 26.5%]; RR, 1.25 [95% CI, 0.98 to 1.57]; P = 0.061). The biochemical pregnancy rate was higher in the LE group (71.0% vs 57.0%; absolute difference, 14.0% [95% CI, 0.8 to 27.2%]; RR, 1.25 [95% CI, 1.01 to 1.54]; P = 0.039). No significant differences were observed for the other secondary outcomes. The LE group showed higher biochemical pregnancy and live birth rates in the normal weight and normal androgen subgroups. Pregnancy outcomes were similar in the overweight and hyperandrogenic subgroups.</p><p><strong>Conclusion: </strong>There were no statistically significant differences in clinical pregnancy rates between the LE and HRT cycles for FET in women with PCOS. However, the LE protocol may be a preferable option to HRT for women with normal weight or normal androgen levels.</p><p><strong>Clinical trial registration: </strong>This study was registered at Chinese Clinical Trial Registry ( http://www.chictr.org.cn/ ; ChiCTR-IOR-17014124).</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":"1907-1915"},"PeriodicalIF":3.2,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144008742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Semen HPV and IVF: insights from infection prevalence to embryologic outcomes.","authors":"Jynfiaf Francis, Panagiotis Tsiartas, Julius Hreinsson, Maria Andersson, Jonas Hermansson, Periklis Gogas, Theophilos Papadimitriou, Cecilia Kärrberg, Mats Brännström, Randa Akouri","doi":"10.1007/s10815-025-03513-6","DOIUrl":"10.1007/s10815-025-03513-6","url":null,"abstract":"<p><strong>Purpose: </strong>Human papillomavirus (HPV), the most common sexually transmitted infection, has been proposed as a potential factor in male infertility. This study aimed to assess the prevalence of HPV in semen samples from men undergoing in vitro fertilization (IVF) in Sweden and evaluate its association with semen parameters and embryological outcomes.</p><p><strong>Methods: </strong>This prospective cohort study was conducted at Sahlgrenska University Hospital, Gothenburg, Sweden, between January 2023 and February 2024. Men (n = 246) undergoing IVF provided fresh semen samples for HPV DNA testing using real-time PCR. Semen analysis followed WHO guidelines, and fertilization and embryo quality assessments were conducted according to the Istanbul Consensus. Machine learning (ML) models were employed to predict fertilization and blastocyst formation outcomes.</p><p><strong>Results: </strong>HPV was detected in 8.9% of semen samples. No significant differences in semen parameters were found between HPV-positive and HPV-negative men. However, in the non-male infertility subgroup, HPV-positive men had significantly higher total motility (median 65 vs. 60%, p = 0.021) and progressive motility (median 65 vs. 55%, p = 0.016). Similarly, in the unexplained infertility subgroup, progressive motility was higher in HPV-positive men (median 60 vs. 50%, p = 0.033). No significant differences were found in fertilization or blastocyst formation rates, and ML analysis confirmed that HPV presence did not influence predictive model accuracy.</p><p><strong>Conclusion: </strong>HPV is detectable in the semen of a notable number of men undergoing IVF, but its presence does not significantly impact fertilization or embryo development. These findings suggest that routine HPV screening in semen may not be necessary for predicting IVF outcomes.</p><p><strong>Trial registration: </strong>The study was registered on ClinicalTrials.gov (ID: NCT06161727).</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":"2053-2066"},"PeriodicalIF":3.2,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144119475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Focusing on the current situation and future challenges of fertility preservation for gynecologic cancer patients in China.","authors":"Tiantaixi Tu","doi":"10.1007/s10815-025-03534-1","DOIUrl":"10.1007/s10815-025-03534-1","url":null,"abstract":"","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144187103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Elective fertility preservation among graduate medical trainees: a survey study of perceived barriers and the impact of employer-sponsored financial benefits.","authors":"Sydney Daviskiba, Maheen Irshad, Samantha Katz, Monique Swain","doi":"10.1007/s10815-025-03531-4","DOIUrl":"https://doi.org/10.1007/s10815-025-03531-4","url":null,"abstract":"<p><strong>Purpose: </strong>To assess the impact of employer-provided financial support on graduate medical trainees' decision to pursue fertility preservation and to identify perceived barriers to participation in fertility preservation.</p><p><strong>Methods: </strong>An anonymous online survey study addressing perspectives surrounding participation in elective fertility preservation while in training was distributed in August 2024 to residents and fellows in graduate medical programs across the United States.</p><p><strong>Results: </strong>Of 306 medical trainee respondents, 202 (67.1%) had intentionally delayed childbearing due to their medical training. There were 66.4% (n = 200) who reported that their sex/gender impacted their interest in fertility preservation, including 87% (n = 172) of whom had been assigned female at birth. High costs and time constraints of training were the most commonly cited factors (63.1%) negatively impacting the decision to participate in elective fertility preservation. Employer financial support was reported by 32.9% (n = 99) as a factor that would make them more likely to pursue fertility preservation. Trainees who worked an average of 60 to > 80 hours per week had the highest rate of reported intentional delay in childbearing, and the time constraints of training negatively impacted their interest in fertility preservation. Only 24% knew whether coverage for fertility services was mandated in their state, 25.2% were unfamiliar with fertility preservation, and 35.3% did not know how to access fertility services.</p><p><strong>Conclusion: </strong>Medical trainees face significant financial and structural barriers to accessing fertility preservation. Widespread financial benefits and systemic changes in training programs may improve the feasibility of fertility preservation for graduate medical trainees.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144187101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of an improved medium for the preservation of human spermatozoa.","authors":"Alena J Hungerford, Natasha Harrison, Hassan W Bakos, Robert J Aitken","doi":"10.1007/s10815-025-03525-2","DOIUrl":"https://doi.org/10.1007/s10815-025-03525-2","url":null,"abstract":"<p><strong>Purpose: </strong>To create a novel medium that retained human sperm quality following cryopreservation at a higher level than that seen with currently available commercial cryoprotectants.</p><p><strong>Methods: </strong>Cryopreservation was achieved via 1:1 dilution with cryoprotectant followed by slow-programmed freezing. A NaCl-free cryopreservation carrier medium based on the use of histidine as the major osmolyte was designed that was capable of sustaining human sperm motility over 6 days at ambient temperature. This medium was supplemented with ethylene glycol, glycerol, and DMSO to create the basis for a novel cryopreservation medium. Dose-dependent studies with various supplements were then conducted to optimize the effectiveness of this formulation including assessments of vitamin C, EDTA, crocin, zinc, ergothioneine, and myo-inositol, as well as the potential replacement of DMSO by Cyrene™. Post-thaw samples were assessed for motility, vitality, and DNA integrity and then reassessed following sperm isolation with the Felix™ System.</p><p><strong>Results: </strong>The completed cryopreservation formulation comprised 4.5% ethylene glycol, 4.5% glycerol, 1% DMSO in a carrier medium supplemented with 0.4 mM vitamin C, 1 mM EDTA, and 22 mM myo-inositol. Spermatozoa frozen in this medium and isolated using the Felix™ System had significantly greater total motility, progressive motility, vitality, and DNA integrity than spermatozoa frozen in a commercially available product that is widely used in infertility clinics.</p><p><strong>Conclusion: </strong>A novel cryopreservation medium has been developed in this study that represents a significant improvement over existing technologies.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144181445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}