Journal of Assisted Reproduction and Genetics最新文献

{"title":"Exploration of ferroptosis-related biomarkers with prognostic capability in RIF based on WGCNA.","authors":"Tingting Zhou, Qian Zhang, Wenhao Yu, Yuqian Cui, Junhao Yan, Tianxiang Ni, Xiaohua Fu, Junwei Li","doi":"10.1007/s10815-024-03370-9","DOIUrl":"10.1007/s10815-024-03370-9","url":null,"abstract":"<p><strong>Purpose: </strong>To explore the association of ferroptosis with repeated implantation failure (RIF) and prognostic capability of ferroptosis-related genes.</p><p><strong>Methods: </strong>Data in GSE106602 from the GEO database were used for gene co-expression network construction to confirm ferroptosis-related genes compared to gene sets that were downloaded from FerrDB. Then these genes were analyzed for functional enrichment and validated using endometrium samples from our center. ImplantScore and ROC curve were constructed for prognostic correlation analysis.</p><p><strong>Results: </strong>We observed that ferroptosis probably participated in RIF according to bioinformatics analysis on a gene set which exhibited a strong association with RIF from WGCNA. Fifty-four ferroptosis-related genes in the gene set were subsequently verified, and the PPI network was established for underlying interactions among them. There were 23 hub genes with differential expression in RIF and six of them (PML, LCN2, PRKAA1, BACH1, SLC7A11, and CAMKK2) showed significant correlation with implantation outcomes using samples collected from our center. Therefore, we combined the six genes and constructed an ImplantScore whose AUC reached 0.891, higher than the AUC of each single gene, respectively. ImplantScore of six genes with down-regulated expression in the group with failed implantation were much lower than that with successful outcome.</p><p><strong>Conclusion: </strong>Our results demonstrated the potential prognostic functions of ferroptosis-related biomarkers in RIF, which will provide novel perspectives for further research and clinical applications.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":"949-959"},"PeriodicalIF":3.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11950601/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142882153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oocyte maturation defect in women undergoing IVF: contributing factors and effects on mature sibling oocyte outcomes.","authors":"Marga Esbert, Andrés Reig, Agustín Ballestros, Emre Seli","doi":"10.1007/s10815-024-03353-w","DOIUrl":"10.1007/s10815-024-03353-w","url":null,"abstract":"<p><strong>Purpose: </strong>This study aimed to identify demographic and clinical factors associated with low maturation rates and to investigate if the rate of immature oocytes impacts the outcomes of mature sibling oocytes.</p><p><strong>Methods: </strong>Women undergoing their first IVF-ICSI cycle between 2018 and 2022 at a fertility clinic were included. Cycles were classified into five groups according to the proportion of Metaphase II stage oocytes (MII): Null (0% MII, n = 46), Poor (1-25% MII, n = 44), Low (26-50% MII, n = 453), Acceptable (51-75% MII, n = 1641), and Optimal (76-100% MII, n = 2642). Demographic characteristics and clinical outcomes were compared between the five groups. In patients with a Null/Poor maturation rate, subsequent cycle outcomes were also evaluated.</p><p><strong>Results: </strong>A total of 4826 cycles were included in the study; 69,909 oocytes were recovered, and 53,065 were MIIs (75.9%). The Null group was older, had lower levels of anti-Müllerian hormone (AMH), needed more gonadotropins and days of stimulation, had higher follicle stimulating hormone (FSH) levels on day 3, and had less follicles > 15 mm on the day of trigger. When the outcomes of mature oocytes were compared, fertilization, usable blastocyst, aneuploidy, and life birth rates were comparable among groups. A binary logistic regression model using number of oocytes, paternal age, and trigger type with live birth rate endpoint found no differences between the categories and the base line Poor category. When patients whose maturation rate was Null/Poor, 42 (47.0%) carried out a second cycle; the maturation rate increased (56.9 ± 31.5 vs. 11.6 ± 11.2%, P < 0.0001).</p><p><strong>Conclusion: </strong>Our data suggest that poor responders are more likely to have low rates of oocyte maturation. The proportion of immature oocytes does not impact the outcomes of mature sibling oocytes. In patients with Null/Poor maturation in their first cycle, the subsequent cycle is often associated with improved maturation rates.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":"773-780"},"PeriodicalIF":3.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11950596/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142949594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of single versus group culture on cumulus-oocyte complexes from early antral follicles.","authors":"Mohammadreza Ebrahimi, Laura Mara, Sara Succu, Sergio Domenico Gadau, Maria Grazia Palmerini, Fabrizio Chessa, Maria Dattena, Francesca D Sotgiu, Valeria Pasciu, Ilaria Antenisca Mascitti, Guido Macchiarelli, Alberto Maria Luciano, Fiammetta Berlinguer","doi":"10.1007/s10815-025-03404-w","DOIUrl":"10.1007/s10815-025-03404-w","url":null,"abstract":"<p><strong>Purpose: </strong>This study aimed to evaluate the effectiveness of single versus group culture strategies for cumulus-oocyte complexes (COCs) derived from early antral follicles (EAFs), with the goal of optimizing culture conditions to increase oocyte availability for assisted reproductive technologies.</p><p><strong>Methods: </strong>COCs isolated from EAFs (350-450 µm) from sheep ovaries were cultured in TCM199 medium supplemented with 0.15 µg/mL Zn⁺⁺ as zinc sulfate, 10^-4 IU/mL FSH, 10 ng/mL estradiol, 50 ng/mL testosterone, 50 ng/mL progesterone, and 5 µM Cilostamide. After 5 days of long in vitro culture (LIVC), COCs underwent in vitro maturation. This study investigated the effects of single and group culture conditions on COCs, focusing on morphology (integrity of oocyte-granulosa cell complex), viability, oocyte diameter, chromatin configuration, and ultrastructure. Additional factors influencing developmental competence were assessed, including global transcriptional activity, gap junction communication, and meiotic competence. Intracellular reactive oxygen species (ROS) levels and mitochondrial activity were also measured.</p><p><strong>Results: </strong>No significant differences were observed between groups in terms of morphology, viability, oocyte diameter, chromatin configuration, ROS levels, or mitochondrial activity. However, group culture resulted in ultrastructural changes, with a notable reduction in global transcriptional activity, an increase in active gap junctions, and a higher rate of meiosis resumption (p < 0.01).</p><p><strong>Conclusion: </strong>Overall, group culture of COCs derived from sheep EAFs promoted meiosis resumption, suggesting that this approach could improve in vitro culture techniques, increase the availability of mature gametes, and support fertility preservation programs.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":"961-976"},"PeriodicalIF":3.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11950561/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143052235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oocyte/zygote/embryo maturation arrest: a clinical study expanding the phenotype of NOBOX variants.","authors":"Annelore Van Der Kelen, Sophie Uyttebroeck, Stefanie Van de Voorde, Ludovica Picchetta, Ingrid Segers, Jelle Vlaeminck, Bart Johan H Dequeker, Philippe Giron, Antonio Capalbo, Willem Verpoest, Frederik J Hes, Pieter Verdyck, Alexander Gheldof","doi":"10.1007/s10815-025-03402-y","DOIUrl":"10.1007/s10815-025-03402-y","url":null,"abstract":"<p><strong>Purpose: </strong>Primary ovarian insufficiency (POI) is an important cause of female infertility, stemming from follicle dysfunction or premature oocyte depletion. Pathogenic variants in genes such as NOBOX, GDF9, BMP15, and FSHR have been linked to POI. NOBOX, a transcription factor expressed in oocytes and granulosa cells, plays a pivotal role in folliculogenesis. Loss-of-function variants in NOBOX are reported in 1-2% of POI women. This study aims to describe the association of novel NOBOX variants with a distinct oocyte, zygote, and embryo maturation arrest (OZEMA) phenotype in infertile women.</p><p><strong>Methods: </strong>Three unrelated women experiencing OZEMA and undergoing multiple in vitro fertilization (IVF) cycles present with a germline NOBOX variant. The detected variants were cross-referenced with a large genetic database to explore their association with IVF outcomes.</p><p><strong>Results: </strong>A heterozygous NM_001080413.3 (NOBOX): c.1797_1798del, p.(Cys600Phefs*27) variant was detected in a woman with oocyte maturation arrest. Another heterozygous variant, NM_001080413.3 (NOBOX): c.1849C > T, p.(His617Tyr), was detected in two women experiencing embryonic developmental arrest. Segregation analysis in one of the two latter families revealed the presence of the p.(His617Tyr) variant in an affected sister, while the two fertile sisters did not carry this variant. Furthermore, the p.(His617Tyr) variant was found in three women in a large database of whom two presented with an embryonic developmental arrest.</p><p><strong>Conclusion: </strong>Two heterozygous NOBOX variants were identified in women with an OZEMA phenotype. Where pathogenic NOBOX variants are typically linked to POI, our clinical findings suggest that NOBOX plays a role in subsequent oocyte maturation and early embryo development.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":"763-771"},"PeriodicalIF":3.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11950555/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143052719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Family misfortune caused by hereditary bias: a reflection on mitochondrial disease diagnosis in a family.","authors":"Yan Meng, Lin Wan, Xi-Ying Yang, Fang Han, Meng-Na Zhang, Wen He, Xiu-Yu Shi, Guang Yang, Li-Ping Zou, Lin-Yan Hu","doi":"10.1007/s10815-025-03399-4","DOIUrl":"10.1007/s10815-025-03399-4","url":null,"abstract":"<p><strong>Aim: </strong>Assisted reproductive technology (ART) is an invaluable strategy for preventing the inheritance of genetic disorders and promoting the birth of healthy children. Nevertheless, the general public's limited understanding of genetics and low awareness of available services obstruct effective utilization of genetic counseling. Our analysis of a family affected by mitochondrial genetic disease aims to improve public understanding of genetic knowledge and the importance of genetic counseling.</p><p><strong>Methods: </strong>We gathered comprehensive data on a family with mitochondrial disease and scrutinized the genetic sequencing and diagnostic procedures used to identify mitochondrial disease within the family.</p><p><strong>Results: </strong>In a case involving a family with two daughters, both began to exhibit symptoms such as abnormal gait, myodystonia, and excessive fatigue at the age of 4. These symptoms were incorrectly assumed to be paternally inherited, as the mother believed the father had a mild intellectual disability. As a result, the family opted for ART, specifically in vitro fertilization (IVF) with donor sperm, without thorough genetic counseling or a conclusive diagnosis for the children. Despite these precautions, the son born from IVF presented with symptoms mirroring his sisters' at the age of 6, including typical MRI abnormal signals in the bilateral basal ganglia. Furthermore, the eldest daughter's naturally conceived child also started to show identical symptoms by the age of 3. Subsequent genetic testing revealed a homoplasmic pathogenic mutation in the MT-ND6 gene (m.14459G>A), confirming that the dystonia was maternally inherited, with the mother exhibiting an 89.2% heteroplasmic variation in the same gene.</p><p><strong>Conclusions: </strong>This case study demonstrates the significant consequences of a lack of genetic knowledge and prevailing misconceptions when applying ART. It underscores the urgent need to bolster genetic literacy and emphasizes the vital importance of informed decision-making within genetic healthcare services.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":"991-996"},"PeriodicalIF":3.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11950491/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143399404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Global, regional, and national burden of infertility, 1990-2021: systematic analysis of the Global Burden of Disease Study 2021.","authors":"Jialyu Huang, Yajie Liao, Maolin Yu, Jing Zhu, Ziqi Yang, Haisheng Wu, Yan Zhao","doi":"10.1007/s10815-025-03429-1","DOIUrl":"10.1007/s10815-025-03429-1","url":null,"abstract":"","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":"1025-1028"},"PeriodicalIF":3.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11950474/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143458071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acceptance rates and reasons for social oocyte cryopreservation among women: systematic review and meta-analysis.","authors":"Özden Tandoğan, Gözde Küğcümen, İlkay Güngör Satılmış","doi":"10.1007/s10815-025-03425-5","DOIUrl":"https://doi.org/10.1007/s10815-025-03425-5","url":null,"abstract":"<p><p>The aim of this systematic review and meta-analysis was to determine the acceptance rates and reasons for social oocyte cryopreservation (SOC) in the general population (subgroup 1) and healthcare professionals/students (subgroup 2) according to the current literature. Relevant studies published between 2007 and Sept 2023 were identified from electronic databases, including PubMed, EBSCO MEDLINE Complete, Web of Science, Science Direct, Scopus, and CINAHL. Quantitative studies reporting women's acceptance rates and reasons for social oocyte cryopreservation were eligible. A total of 20 quantitative studies were included in this process. Meta-analyses were conducted using random-effects models to evaluate study effect sizes. A total of 20 articles were analyzed. While the acceptance rate of SOC women in the general population was 56.5% (95% CI = 47.8-64.9%; τ² = 0.98, df = 20; p < 0.001), this rate was between 42 and 66% in female healthcare professionals/students (p > 0.05; I² = 98.46%; df = 20; p < 0.001). Among the acceptable reasons for SOC, cost-recovery (67.9%, 95% CI = 58-76%, I² = 97.88%, τ² = 0.95, df = 6; p < 0.001) and inability to find a suitable partner (45.7%, 95% CI = 32.6-59.5%; I² = 97.96%, df = 10; p < 0.001) stand out. Acceptance rates did not differ significantly between the general population and healthcare professionals/students' inability to find a suitable partner (p > 0.05). The meta-analysis shows that more than half of women accept SOC, with the acceptance rate increasing in the absence of a partner and if the cost is affordable.Trial registration CRD42023455656.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143537208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling clinic variation in utilization of ICSI, PGT, and blastocyst transfer: a comprehensive study using the SART database (2014-2020).","authors":"Andrew Claffey, Anh Ngo, Donald McIntire, Kathleen Doody, Kevin Doody","doi":"10.1007/s10815-025-03413-9","DOIUrl":"10.1007/s10815-025-03413-9","url":null,"abstract":"<p><strong>Purpose: </strong>Many national recommendations have been made regarding practice patterns of assisted reproductive technology utilization (intracytoplasmic sperm injection (ICSI), preimplantation genetic testing (PGT), and blastocyst stage embryo transfer). This study seeks to assess individual clinic changes in practice behaviors from 2014 to 2020 in response to national guidelines.</p><p><strong>Methods: </strong>This retrospective cohort study examined data from individual clinics using the Society of Assisted Reproductive Technology Clinical Outcome Reporting System (SART-CORS) database. Each clinic was weighed individually and equally regardless of clinic volume to avoid skewing national data with larger-volume clinics. The trends of ICSI, PGT, and blastocyst stage embryo transfer were examined across five age groups (< 35, 35-37, 38-40, 40-42, and > 42).</p><p><strong>Results: </strong>ICSI usage from 2014 to 2020 increased in all age groups (p < 0.001). In 2020, more than half the clinics in this study utilized ICSI in ≥ 90% of IVF cycles (per oocyte retrieval). Utilization of prolonged embryo culture increased in all age groups across the seven years of this study, with a more extensive adoption of this practice in patients ≤ 40 years (p < 0.001). Between 2014 and 2020, PGT usage increased by 3-4 times across all age groups (p < 0.001). By 2020, PGT was performed after egg retrievals in approximately 40 to 50% (mean) of patients < 42 years old.</p><p><strong>Conclusions: </strong>Nationally, the application of all three reproductive technologies (ICSI, PGT, and blastocyst stage transfer) has drastically increased. The authors suspect that these technology expansions are interrelated and may be associated with the rise of rates of PGT.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":"799-807"},"PeriodicalIF":3.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11950454/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143255679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"B cell translocation gene 2 expression levels in human granulosa cells is negatively associated with in vitro fertilization/intracytoplasmic sperm injection outcomes: a pilot study.","authors":"Jiahuan Luo, Huawei Wang, Ling Zhou, Longda Wang, Jinyuan Wang, Mengjie Song, Yulin Cheng, Meng Rao, Shuhua Zhao, Li Tang","doi":"10.1007/s10815-025-03391-y","DOIUrl":"10.1007/s10815-025-03391-y","url":null,"abstract":"<p><strong>Purpose: </strong>This study aimed to monitor the expression of B-cell translocation gene 2 (BTG2) in granulosa cells of patients undergoing IVF/ICSI with respect blastocyst quality outcomes.</p><p><strong>Methods: </strong>We recruited 181 women undergoing IVF/ICSI cycles for infertility. Granulosa cells were extracted from follicular fluid. BTG2 expression level of granulosa cells were stratified into tertiles (low, middle, and high), and the patients of each tertile were compared for outcome indicators by Kruskal-Wallis analysis. Spearman's correlation analyses were used to evaluate the correlation between BTG2 mRNA levels and outcome indicators. Generalized linear models and generalized additive models with smoothing splines were used to adjust for potential confounders.</p><p><strong>Results: </strong>Patients in the low BTG2 tertile had higher oocyte retrieval, fertilization, blastocyst formation, and high-quality blastocyst rates than those in the high BTG2 tertile. Patients in the high BTG2 tertile exhibited a downward trend in implantation and clinical pregnancy rates compared to those in the low or middle BTG2 tertiles, whereas the early pregnancy loss rate showed an upward trend, although the difference was not significant. After adjusting for confounding factors, the expression level of BTG2 was negatively correlated with oocyte retrieval, blastocyst formation, and high-quality blastocyst rates. Stratified analysis of AMH > 4 ng/ml showed elevated BTG2 expression was associated with reduced oocyte retrieval, fertilization, cleavage, blastocyst formation, and high-quality blastocyst rates. No differences in these outcomes were observed in patients with AMH ≤ 4 ng/ml.</p><p><strong>Conclusion: </strong>In women with high AMH levels (> 4 ng/ml) elevated BTG2 expression in granulosa cells was associated with poor quality blastocyst outcomes.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":"909-922"},"PeriodicalIF":3.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11950548/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143038906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Growth hormone improves the pregnancy outcomes in poor ovarian responders undergoing in vitro fertilization: an umbrella review.","authors":"Yongmei Liu, Fengxing Ding, Yuan Yang, Bin Ma","doi":"10.1007/s10815-025-03389-6","DOIUrl":"10.1007/s10815-025-03389-6","url":null,"abstract":"<p><p>Poor ovarian response (POR) significantly impacts the success of assisted reproductive technology (ART), and growth hormone (GH) has been proposed as an adjuvant treatment to improve outcomes in POR patients undergoing in vitro fertilization (IVF). A systematic review and meta-analysis were conducted to evaluate the effectiveness of GH in enhancing pregnancy outcomes, registering a protocol on PROSPERO and searching multiple databases up to September 2023. Twelve systematic reviews/meta-analysis and 20 randomized controlled trials (RCTs) involving 1984 patients were included. Quality assessment was performed using AMSTAR 2, GRADE, and RoB tools. The meta-analysis revealed that GH significantly increased live birth rates [OR=1.80, 95% CI (1.22, 2.64)] and clinical pregnancy rates [OR=1.92, 95% CI (1.51, 2.43)] compared to the control group. Subgroup analysis indicated that administering 5-10 IU/d of GH combined with a long protocol during the middle and late follicular stages maximized these benefits. Despite these promising findings, most outcome indicators exhibited low-quality evidence, highlighting the need for improved research standards to ensure solid evidence supports treatment strategies for POR, thereby promoting reliable application of GH in IVF treatments.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":"721-736"},"PeriodicalIF":3.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11950600/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143038912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}