Journal of Assisted Reproduction and Genetics最新文献

{"title":"Establishment and application of an RNA-seq-based predictive model for the hourly precision window of implantation (WOI) in patients with recurrent implantation failure.","authors":"Aihua He, Tianli Yang, Sijia Lu, Yangyun Zou, Cheng Wan, Yulin Chen, Jing Zhao, Nenghui Liu, Donge Liu, Yumei Li, Yonggang Wang, Bin Xu, Jie Hao, Jing Fu, Qiong Zhang, Baisheng Wang, Yanping Li","doi":"10.1007/s10815-025-03468-8","DOIUrl":"10.1007/s10815-025-03468-8","url":null,"abstract":"<p><strong>Purpose: </strong>This study aims to establish a single-time point RNA-sequencing-based endometrial receptivity test (rsERT) to provide hourly precision of endometrial WOI for patients with recurrent implantation failure (RIF).</p><p><strong>Methods: </strong>A two-phase study was conducted. In the first phase, a total of 70 patients with successful intrauterine pregnancy after personalized embryo transfer (pET) guided by three-time points rsERT were recruited for modified rsERT. Another 21 patients who underwent the prototype of single-time point rsERT were further included for optimization. In the second phase, another cohort of 574 patients with RIF that was recruited and assigned to the experimental group underwent pET guided by the modified rsERT (n = 261) or the control group underwent conventional ET (n = 313). The positive β-human chorionic gonadotropin (β-hCG), intrauterine pregnancy rate (IPR), implantation rate (IR), ongoing pregnancy rate, and live birth rate of the two groups were analyzed.</p><p><strong>Result: </strong>The modified rsERT provided an hour-based predictive result of endometrial WOI with an average accuracy of 94.51% and sensitivity and specificity of 92.73% and 96.29%, respectively. The positive β-hCG, IPR, IR, ongoing pregnancy rate, and live birth rate of the experimental group were significantly different from those in the control group. After propensity score matching (PSM) control for the confounders, positive β-hCG, IR, and early spontaneous abortion rate showed significant differences.</p><p><strong>Conclusion: </strong>The modified rsERT provided hourly precision WOI prediction using a single-time point endometrial biopsy and pregnancy outcomes were significantly improved, providing an enhanced endometrial receptivity test as an alternative requiring only a single-time point sampling for patients with RIF.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":"2067-2082"},"PeriodicalIF":3.2,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143968122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"GDNF enhances HGF-induced tubulogenesis and organization of Sertoli cell.","authors":"Begum Durkut-Kuzu, Ciler Celik-Ozenci","doi":"10.1007/s10815-025-03493-7","DOIUrl":"10.1007/s10815-025-03493-7","url":null,"abstract":"<p><strong>Purpose: </strong>Hepatocyte growth factor (HGF), secreted by Sertoli cells, activates the c-Met receptor, facilitating seminiferous tubule formation. Glial cell-derived neurotrophic factor (GDNF) promotes embryonic Sertoli cell proliferation and cord formation. This study aimed to investigate whether exogenous GDNF contributes to HGF-induced in vitro organization of Sertoli cells in a Matrigel-based three-dimensional (3D) culture system.</p><p><strong>Methods: </strong>Four experimental groups were established using the 15P-1 Sertoli cell line: control, HGF-treated, GDNF-treated, and combined HGF- and GDNF-treated. Tubular structure length and branching were quantified using image analysis software, while the expression of ZO-1 protein was assessed via immunofluorescence staining in 3D Matrigel-based culture for 5 days. Additionally, Gfrα-1, Ret, Ncam, and Met mRNA expression in Sertoli cells were analyzed. The expression levels of ZO-1, c-MET, and p-c-MET were analyzed in two-dimensional (2D) culture after 5 days.</p><p><strong>Results: </strong>Quantitative analysis revealed a significant increase in the length and branching of tubular-like structures when GDNF was co-administered with HGF, compared to other groups. Additionally, there was a notable increase in ZO-1 protein expression with the combined administration of GDNF and HGF, surpassing levels observed in the control group. Furthermore, co-administration of HGF and GDNF significantly elevated phospho-c-Met levels compared to the control group.</p><p><strong>Conclusion: </strong>Our study reveals that exogenous GDNF enhances the HGF-induced tubular organization of Sertoli cells in vitro. The concurrent administration of GDNF and HGF markedly augments the formation of tubular structures and the expression of the ZO-1 protein, indicating a potential synergistic influence on the organizational and signaling cascades within Sertoli cells.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":"2083-2098"},"PeriodicalIF":3.2,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144119322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial Expression of Concern: PICSI vs. MACS for abnormal sperm DNA fragmentation ICSI cases: a prospective randomized trial.","authors":"Eman Hasanen, Khaled Elqusi, Salma ElTanbouly, Abd ElGhafar Hussin, Hanaa AlKhadr, Hosam Zaki, Ralf Henkel, Ashok Agarwal","doi":"10.1007/s10815-025-03533-2","DOIUrl":"10.1007/s10815-025-03533-2","url":null,"abstract":"","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":"2115"},"PeriodicalIF":3.2,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144159249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Artificial oocyte activation technology as adjuvant therapy for primary ciliary dyskinesia: a report of eight cases and literature review.","authors":"Jiao Liu, Yabing Zhang, Tingting Ji, Hongxing Li, Bin Mao, Xiaoling Ma","doi":"10.1007/s10815-025-03486-6","DOIUrl":"10.1007/s10815-025-03486-6","url":null,"abstract":"<p><strong>Objective: </strong>To explore the clinical application effect and feasibility of intracytoplasmic sperm injection (ICSI) combined with artificial oocyte activation (AOA) technology in the treatment of male infertility caused by primary ciliary dyskinesia (PCD).</p><p><strong>Cases report: </strong>Between April 2022 and April 2024, our hospital's reproductive center treated a total of eight patients diagnosed with PCD and concurrent male infertility. Among them, six patients were treated with ICSI in conjunction with AOA as an adjuvant therapy, with their oocytes being subjected to ionomycin treatment for a duration of 15 min post-ICSI. One patient underwent ICSI alone, while another patient chose to use donor sperm. Ultimately, we assessed the fertilization rates and transferable embryo rates of all patients. The statistical results showed that the six patients who received ICSI combined with AOA achieved an average fertilization rate of 77.9% and a transferable embryo rate of 66.7%. Four of these patients achieved clinical pregnancy and live birth after embryo transfer. In contrast, the patient who received only ICSI had a fertilization rate of 51.7% and a transferable embryo rate of 20%.</p><p><strong>Conclusions: </strong>AOA can be used as an adjuvant treatment in ICSI cycles for patients with PCD accompanied by male infertility. It not only increases the fertilization rate but also potentially improves embryo quality, thereby enhancing the transferable embryo rate.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":"1885-1893"},"PeriodicalIF":3.2,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144003412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of cumulative live birth rate outcomes of four ovarian stimulation protocols in Poseidon groups 3/4 patients with adenomyosis.","authors":"Jiayi Guo, Jintao Peng, Yajie Chang, Yanfang Wang, Xiaoyan Liang, Rui Xiang","doi":"10.1007/s10815-025-03488-4","DOIUrl":"10.1007/s10815-025-03488-4","url":null,"abstract":"<p><strong>Purpose: </strong>To compare the effects of the gonadotrophin-releasing hormone (GnRH) agonist protocol, GnRH antagonist protocol, progestin-primed ovarian stimulation (PPOS) protocol, and mild stimulation/natural cycle protocol on the cumulative live birth rate (CLBR) in patients with adenomyosis and Poseidon group 3/4.</p><p><strong>Methods: </strong>A total of 1090 patients diagnosed with adenomyosis and Poseidon groups 3/4 group who underwent in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI) from January 2013 to December 2020 were included. Demographic characteristics, laboratory indicators, and clinical outcomes of the four ovarian stimulation protocols were compared. The primary outcome was the CLBR, with binary logistic regression analysis used to explore the factors influencing CLBR.</p><p><strong>Results: </strong>Among the patients with adenomyosis and Poseidon groups 3/4, the CLBR in GnRH agonist protocol group was higher than that in PPOS protocol group (45.78% vs. 22.33%, P = 0.005). Binary logistic regression analysis revealed that GnRH agonist protocol had a higher CLBR compared to PPOS protocol (adjusted odds ratio (aOR) 3.26, 95% confidence interval (CI): 1.56-6.83, P = 0.002), while no significant differences were found when comparing PPOS protocol with GnRH antagonist protocol (P = 0.093) and mild stimulation/natural cycle protocol (P = 0.125). In the < 35 years subgroup, the CLBR of GnRH agonist protocol was significantly higher (55.26% vs. 33.82%; P = 0.032), which wasn't observed in elder subgroups (P > 0.05).</p><p><strong>Conclusion: </strong>For patients with both adenomyosis and Poseidon groups 3/4, GnRH agonist protocol showed a higher CLBR, especially in patients < 35 years (Poseidon group 3).</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":"1939-1947"},"PeriodicalIF":3.2,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143986297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical application of preimplantation genetic testing based on low-coverage next-generation sequencing with linkage analyses in hereditary hearing loss families.","authors":"Wenjing Wang, Jing Guan, Minyue Ma, Xiaonan Wu, Rui Zhou, Wenkai Ji, Manman Dou, Xiaohui Zhao, Guohui Chen, Lan Lan, Jian Sun, Yuan Gao, Hongmei Peng, Qiuju Wang","doi":"10.1007/s10815-025-03504-7","DOIUrl":"10.1007/s10815-025-03504-7","url":null,"abstract":"<p><strong>Purpose: </strong>In order to explore the clinical effect of preimplantation genetic testing (PGT) in hereditary hearing loss (HHL), we explore the related factors affecting the pregnancy outcome of PGT of hereditary hearing loss and provide more evidence for clinical work.</p><p><strong>Methods: </strong>From January 2015 to April 2024, we select 288 couples of child-bearing age who are at risk of conceiving children with HHL from 1444 pedigrees in Chinese Deafness Genome Project (CDGP). After genetic counseling, 19 couples elected to undergo PGT. The embryo genotypes were diagnosed by low-coverage sequencing combined with SNP linkage analysis, and followed up during pregnancy and after delivery.</p><p><strong>Results: </strong>The 19 couples include variants of autosomal recessive hearing loss gene GJB2, SLC26A4, USH2A, CDH23, and autosomal dominant hearing loss gene MITF, WFS1, and GSDME. The 135 embryos from the 19 couples were cultured in vitro, 93.33% (126/135) embryos got reliable genetic diagnosis, and nine embryos (6.67%) had no diagnosis. The depth of embryonic 2-3 × WGS of 205 human hearing loss genes are sufficient. Eleven women got pregnancy, and eight newborns with normal hearing have been delivered through assisted reproduction; clinical pregnancy rate was 57.89% (11/19). Pregnancy outcome is associated with the female age (P = 0.037), male age (P = 0.015), and number of transferable blastocysts obtained (P = 0.000) in per ART cycle.</p><p><strong>Conclusions: </strong>PGT based on low-coverage next-generation sequencing with linkage analyses can block the transmission of deafness-related mutations to offspring. 2-3 × depth of embryo sequencing data enabled a credible testing of 205 deafness-related mutations loci. Couple age and number of retrieved oocytes are related to pregnancy outcome and can be considered prognostic indicators of PGT of HHL.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":"1989-2002"},"PeriodicalIF":3.2,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144101851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Human menopausal gonadotropin (HMG) combined different doses of letrozole for treating anovulatory infertility in patients with polycystic ovary syndrome: a randomized controlled trial.","authors":"Jingyi Li, Yuan Peng, Xin Dai, Ling Zhang, Xuefeng Long, Xiuping Wang, Weiwei Li, Yuqing Fang, Wenqian Xiong, Yi Liu","doi":"10.1007/s10815-025-03455-z","DOIUrl":"10.1007/s10815-025-03455-z","url":null,"abstract":"<p><strong>Objective: </strong>To optimize ovulation induction protocols for infertile women with PCOS, ovulation effect and adverse reactions of different doses of letrozole (2.5 vs 5.0 mg) combined sequentially HMG therapy were compared in infertility PCOS patients.</p><p><strong>Methods: </strong>This open-label randomized controlled trial (RCT) included 174 infertile women aged 18-40 who met the Rotterdam criteria for PCOS at the Wuhan Union Hospital of China from May 2021 to January 2022. They were randomly assigned at a 1:1 ratio to 2.5 mg LE or 5.0 mg LE on cycle days 3-7 with sequential HMG injections (n = 87 for each).</p><p><strong>Results: </strong>There is no difference in ovulation rate between LE (2.5 mg) + HMG group and LE (5.0 mg) + HMG group in infertile women with PCOS (85.1 vs 85.1%). The ongoing pregnancy rate was no different between the two groups (33.3 vs 25.3%). The percentage of type B endometrial tissues on HCG injection day was higher in the LE (2.5 mg) + HMG group (88.5% vs 69.0%). The monofollicular development rate was significantly higher in the LE (2.5 mg) + HMG group (67.8% vs. 46.0%).</p><p><strong>Conclusions: </strong>Application of 5.0 mg LE followed with HMG does not improve the pregnancy rate compared to 2.5 mg LE in infertile women with PCOS. An increased dose of LE to 5.0 mg may increase the risks of OHSS and multiple pregnancies. Therapy of LE (2.5 mg) + HMG may be a more beneficial and optimal treatment protocol for improving endometrial receptivity and promoting mono-follicle development for patients with PCOS.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":"1895-1906"},"PeriodicalIF":3.2,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143795583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Scoping review on the embryonic developmental potential of tripronuclear zygotes and their use for clinical and research purposes.","authors":"Anne Mayeur, Khadija Ouaziz, Brian Sperelakis-Beedham, Paula Rubens, Nadine Gigarel, Julie Steffann, Nelly Frydman","doi":"10.1007/s10815-025-03467-9","DOIUrl":"10.1007/s10815-025-03467-9","url":null,"abstract":"<p><p>We reviewed the published literature on human 3PN zygotes, focusing on their developmental potential, genetic status, and possible correction, either spontaneous (autocorrection) or through micromanipulation. A total of 29 articles published between 2000 and 2023 were deemed eligible for this scoping review. Significant differences exist between the developmental potential of bipronucleated zygotes and 3PN zygotes, with the latter showing a lower blastulation rate and altered cleavage patterns, and this is related to the fertilization method used. 3PN embryos obtained by conventional IVF are most often diandric, and arrest their development more quickly with a lower blastulation rate, compared to those obtained by ICSI. This result may be linked to the number of centrioles establishing the first cytokinesis. Autocorrection can occur in 3PN zygotes, resulting in diploid embryos, and there have been reports of live births following the transfer of 3PN embryos. Induced diploidization through pronuclear removal by micromanipulation is technically feasible and associated with good survival rates. Because tripronuclear embryos are destined to be discarded, they can serve as a source of additional embryos for research purposes without major ethical concerns. Furthermore, the transfer of 3PN embryos, corrected either spontaneously or by micromanipulation, holds the potential to increase the pool of embryos eligible for transfer in IVF centers. However, this cannot be considered without genetic screening aiming to evaluate diploidy, euploidy, and heteroparental inheritance, and no studies have yet evaluated these three genetic aspects comprehensively. Further research is essential to fully understand the physiology and potential of corrected 3PN zygotes in the fields of assisted reproduction and research.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":"1743-1753"},"PeriodicalIF":3.2,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144026154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insights gained from a cleavage-stage embryo freeze-all policy in in vitro maturation cycles.","authors":"Ingrid Segers, Lisbet Van Landuyt, Maria-Petra Agius, Linde Mostinckx, Wilfried Cools, Celine Schoemans, Koen Wouters, Neelke De Munck, Michel De Vos","doi":"10.1007/s10815-025-03469-7","DOIUrl":"10.1007/s10815-025-03469-7","url":null,"abstract":"<p><strong>Purpose: </strong>Cycles of in vitro maturation (IVM) of oocytes show asynchrony between embryo development and endometrial receptivity. Hence, elective embryo vitrification/warming (V/W) for embryo transfer (FET) is routinely performed. However, clinical outcomes after IVM are lower compared to conventional ovarian stimulation, mainly due to lower embryo quality. Vitrification at cleavage stage, rather than blastocyst stage, is used to optimize embryo utilization while maintaining acceptable pregnancy rates. The aim of this study is to ascertain the vulnerability to V/W of IVM-derived cleavage-stage embryos and to identify characteristics that predict pregnancy.</p><p><strong>Methods: </strong>In this single-center retrospective cohort study, 442 day-3 IVM-derived embryos from PCOS patients were investigated. Cell survival upon warming, cell cycle progression during overnight culture and clinical outcome in 425 FET cycles were analyzed.</p><p><strong>Results: </strong>From 442 V/W embryos, 85% were fully intact. Cell loss reduced the cell cycle progression after overnight culture of V/W embryos (p = 0.047) and tended to lower clinical pregnancy rates (16% vs 23%, p = 0.22) compared to intact embryos. Better fresh embryo quality was associated with enhanced cell cycle resumption after overnight culture (p < 0.0001). Cell cycle resumption was required for pregnancy to occur. Additionally, the extent of cell cycle progression (OR = 0.439, CI = 0.24-0.78, p < 0.001) and the number of available top-quality embryos (OR = 0.174, CI = 0.04-0.32, p = 0.01) were indicative for success.</p><p><strong>Conclusions: </strong>A clinical pregnancy rate of 23% per FET with V/W IVM-derived cleavage-stage embryo was obtained with cell cycle resumption after overnight culture as dominant predictive factor.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":"1795-1804"},"PeriodicalIF":3.2,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143772539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of acupuncture-related therapies on pregnancy outcomes among women undergoing in vitro fertilization and embryo transfer: a Bayesian network meta-analysis.","authors":"Chengli Bin, Hanzhi Zhong, Shipeng Zhang, Yiping Luo, Jiali Su, Maoya Li, Shaobin Wei","doi":"10.1007/s10815-025-03489-3","DOIUrl":"10.1007/s10815-025-03489-3","url":null,"abstract":"<p><strong>Background: </strong>This network meta-analysis aimed to assess the efficacy of different acupuncture-related therapies in improving pregnancy outcomes among women undergoing in vitro fertilization and embryo transfer (IVF-ET).</p><p><strong>Methods: </strong>Randomized controlled trials (RCTs) examining acupuncture-related therapies as adjuncts to IVF-ET were systematically searched in eight databases from inception until January 15, 2025. Dichotomous outcomes concerning efficacy were evaluated as odds risk (OR) and continuous data as mean difference (MD) and 95% credible intervals (CrI) utilizing R 4.1.2 and Stata 16.1.</p><p><strong>Results: </strong>Through a comprehensive literature search, we ultimately identified 96 RCTs that involved 14,736 participants and 15 interventions in this systematic analysis. Based on the clinical pregnancy rate outcome, warm acupuncture for three menstrual cycles before oocyte retrieval (WA-TTP, OR 3.56, 95% CrI 2.05 to 6.25, low certainty, SUCRA = 89.54%), acupuncture combined with moxibustion for three menstrual cycles before oocyte retrieval (AC + M-TTP, OR 3.31, 95% CrI 1.05 to 11.77, low certainty, SUCRA = 78.70%), and acupuncture for one menstrual cycle before oocyte retrieval (AC-OTP, OR 2.69, 95% CrI 1.76 to 4.09, moderate certainty, SUCRA = 77.98%) demonstrated potential superiority compared to false acupuncture or no treatment (F/N). Significant subgroup differences between clinical pregnancy rates were observed by subgroup analysis.</p><p><strong>Conclusion: </strong>Acupuncture-related therapies can potentially enhance clinical pregnancy rates among women undergoing IVF-ET, with WA-TTP, AC + M-TTP, and AC-OTP demonstrating potential superiority. AC-TTP demonstrated a greater efficacy in improving live birth rates, increasing endometrial thickness, and reducing pulsation index. Our findings emphasize that acupuncture-related therapies with a limited number of sessions before or after embryo transfer show minimal clinical benefit except auricular acupressure.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":"1925-1938"},"PeriodicalIF":3.2,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143992941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}