Journal of Assisted Reproduction and Genetics最新文献

{"title":"Aneuploidy-driven gene expression profiling in human blastocysts: insights from RNA-Seq analysis.","authors":"Masood Bazrgar, Fattaneh Khajehoseini, Poopak Eftekhari-Yazdi, Mohammad Reza Bakhtiarizadeh, Hamid Gourabi, Parishad Saei, Reza Pazhoomand, Shirzad Hosseinishenatal, Reza Mohammadi","doi":"10.1007/s10815-024-03369-2","DOIUrl":"10.1007/s10815-024-03369-2","url":null,"abstract":"<p><strong>Purpose: </strong>Preimplantation aneuploidy in humans is one of the primary causes of implantation failure and embryo miscarriage. This study was conducted to gain insight into gene expression changes that may result from aneuploidy in blastocysts through RNA-Seq analysis.</p><p><strong>Methods: </strong>The surplus embryos of preimplantation genetic testing for aneuploidy (PGT-A) candidate couples with normal karyotype and maternal age < 38 were collected following identical ovarian stimulation protocol. The embryos were selected based on trophectoderm biopsy and array comparative genomic hybridization in three groups: normal group, small chromosomes aneuploidy group (SCA), including single aneuploidy for small chromosomes 16, 20, 21, 22, and other chromosomes aneuploidy group (OCA), including single aneuploidy for other chromosomes.</p><p><strong>Results: </strong>Principal component analysis revealed overall differentiation of transcriptome of the groups, confirming embryo classification. The Gene Ontology indicated that transcription, ubiquitination, autophagy, and DNA repair pathways were upregulated in aneuploid embryos. The overexpression of five genes, UBE2E2 and VPS4A, BUB1B, CDCA8, and COX14 was confirmed by quantitative real-time PCR. Additionally, overexpression was observed in translation and protein synthesis pathways in aneuploid embryos. Mitochondrial pathway upregulation was notable in both SCA and OCA groups, while the apoptosis pathway was overexpressed only in the OCA group. Only cellular lipid synthesis pathway differed between SCA and OCA, the two aneuploid groups.</p><p><strong>Conclusions: </strong>This study highlights the impact of aneuploidy on the gene expression in blastocysts independent of aneuploidy type and paves the way for understanding the molecular mechanisms underlying the generation of aneuploidy.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":"885-896"},"PeriodicalIF":3.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11950541/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142927418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Predicting factors of ovarian responses in infertile women with polycystic ovary syndrome undergoing IVF/ICSI.","authors":"Qiaoling Wang, Jingwen Lang, Yunqing Zhi, Xiuxian Zhu, Yonglun Fu","doi":"10.1007/s10815-024-03386-1","DOIUrl":"10.1007/s10815-024-03386-1","url":null,"abstract":"<p><strong>Purpose: </strong>Women with polycystic ovary syndrome (PCOS) show greater heterogeneity in ovarian responses during ovarian stimulation. We aimed to investigate the potential predicting factors among individualized basic parameters that affect poor or hyper ovarian responses in PCOS patients.</p><p><strong>Methods: </strong>We retrospectively screened 2058 women with PCOS who underwent their first cycle of in vitro fertilization/intracytoplasmic sperm injection. Spearman correlation analysis and multivariable linear regression model were applied to screen potential variables impacting the number of oocyte retrieved. Further, women with PCOS were divided into poor, sub-optimal, optimal, and hyper responders based on oocyte-retrieved numbers. Logistic regression model and receiver operating characteristic (ROC) curve were used to testify the predicting effect of screened parameters on ovarian response.</p><p><strong>Results: </strong>Multivariable linear regression showed that body mass index (BMI) and follicle-stimulating hormone (FSH) were significantly negatively correlated with oocyte numbers, while luteinizing hormone and anti-Müllerian hormone (AMH) showed a positive correlation. Logistic regression model showed that high BMI (RR: 1.141, 95% CI: 1.090, 1.195) and FSH (RR: 1.161, 95% CI: 1.043, 1.293) were risk factors for poor and sub-optimal ovarian response, but not for hyper response. High AMH level was a risk factor (RR: 1.118, 95% CI: 1.075, 1.163) for hyper ovarian response. The optimal cutoff value was BMI = 23.25 kg/cm², FSH = 6.375 IU/L, and AMH = 9.8 ng/mL, respectively.</p><p><strong>Conclusions: </strong>Individualized basic parameters including BMI, FSH, and AMH are crucial for predicting ovarian response of women with PCOS, providing valuable information for formulating personalized diagnosis and treatment plans.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":"865-874"},"PeriodicalIF":3.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11950575/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142978558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"miR-361-5p regulates SLC25A24 to maintain mitochondrial function and alleviate granulosa cell dysfunction in diminished ovarian reserve.","authors":"Jinyuan Xu, Yan Jia","doi":"10.1007/s10815-024-03349-6","DOIUrl":"10.1007/s10815-024-03349-6","url":null,"abstract":"<p><strong>Background: </strong>The aim of this study was to investigate the role of miR-361-5p (a tumor suppressor) in regulating granulosa cell function by targeting SLC25A24, a key mitochondrial protein, to uncover potential therapeutic targets for diminished ovarian reserve (DOR).</p><p><strong>Methods: </strong>This study included patients undergoing assisted reproductive technology treatment at our hospital. Granulosa cells were isolated from follicular fluid, and KGN cells were used for in vitro experiments. miR-361-5p and SLC25A24 expression levels were manipulated using miRNA mimics and inhibitors, and their effects on cell viability, apoptosis, and mitochondrial function were assessed. Techniques employed included qRT-PCR, Western blot analysis, ELISA, JC-1 staining, and dual-luciferase reporter assays. Key quantitative metrics included changes in mitochondrial DNA (mtDNA), ATP production, and reactive oxygen species (ROS) levels.</p><p><strong>Results: </strong>miR-361-5p expression was significantly lower in DOR patients' granulosa cells compared to controls (P < 0.01). miR-361-5p inhibition markedly decreased KGN cells viability and increased apoptosis (P < 0.01), while miR-361-5p overexpression had the opposite effects (P < 0.01). SLC25A24 expression was inversely correlated with miR-361-5p levels, and its knockdown reversed the effects of miR-361-5p inhibition. Additionally, miR-361-5p modulation significantly affected mitochondrial function, with its overexpression reducing ROS levels and increasing ATP production (P < 0.01).</p><p><strong>Conclusion: </strong>miR-361-5p plays a pivotal role in maintaining mitochondrial function and reducing KGN cells dysfunction by targeting SLC25A24. These findings offer new insights into the molecular mechanisms of DOR and highlight miR-361-5p as a potential therapeutic target to enhance ovarian reserve and improve fertility outcomes.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":"923-936"},"PeriodicalIF":3.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11950524/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142983559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Patient perspectives after receiving simulated preconception polygenic risk scores (PRS) for family planning.","authors":"Maria Katz, Noor Siddiqui, Barry Behr, Dhruva Chandramohan, Qinnan Zhang, Funda Suer, Yuntao Xia, Benjamin Podgursky","doi":"10.1007/s10815-025-03397-6","DOIUrl":"10.1007/s10815-025-03397-6","url":null,"abstract":"<p><strong>Purpose: </strong>The study investigates patient perspectives on the use of Preimplantation Genetic Testing for Polygenic disease (PGT-P) to select embryos with lower risks for common polygenic diseases. Participant responses and attitudes were evaluated after receiving simulated embryo PRS generated from their personal genetic profile.</p><p><strong>Methods: </strong>Couples seeking OB/GYN or Reproductive Endocrinology and Infertility (REI) care with an interest in genetic risks for common diseases in their prospective children participated. A tool provided PRS scores for 11 conditions, using parental DNA to simulate genetic risks for hypothetical embryos produced during IVF. Participants received counseling, reviewed results online, and completed a post-test survey. Feedback from 90 participants assessed understanding and attitudes toward PRS use in IVF.</p><p><strong>Results: </strong>Participants were overall more supportive of screening embryos for childhood-onset diseases (80%) compared to adult-onset conditions (63%); however, among specific diseases, participants expressed the greatest interest in screening for adult-onset cognitive disorders (Schizophrenia, 86%, Alzheimer's disease, 82%). Participant-free responses noted the importance of personalized counseling and participants not of European ancestry expressed frustration with limited PRS applicability. Negative reactions to testing (nervousness or anxiety 5%, regret 2%) were explored.</p><p><strong>Conclusions: </strong>The findings examine the receipt of simulated embryo PRS in a patient population in which support for using PRS during embryo prioritization is high. Positive patient interest was consistent with other US studies; as prior studies identify significant clinician discomfort, these results highlight the need for comprehensive genetic counseling and inclusive stakeholder input in shaping guidelines for PRS during IVF.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":"997-1013"},"PeriodicalIF":3.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11950577/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143390975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prenatal diagnosis following preimplantation genetic testing (PGT): recommendations of the Italian Society of Human Genetics (SIGU).","authors":"Francesca Romana Grati, Antonio Capalbo, Ilaria Gabbiato, Paola Battaglia, Maria Carla Pittalis, Domenico Bizzoco, Laura Cardarelli, Valentina Gatta, Fortunato Lonardo, Antonio Novelli, Laura Bernardini, Daniela Zuccarello","doi":"10.1007/s10815-024-03358-5","DOIUrl":"10.1007/s10815-024-03358-5","url":null,"abstract":"<p><p>This document aims to provide good practice recommendations in order to support maternal-foetal medicine specialists, clinical geneticists and clinical laboratory geneticists in the management of pregnancies obtained after the transfer of an embryo tested with preimplantation genetic testing (PGT). It was drafted by geneticists expert in preimplantation genetics and prenatal genetic diagnosis belonging to the \"Working Group in Cytogenomics, Prenatal and Reproductive Genetics\" of the \"Italian Society of Human Genetics\" (SIGU). In particular, the paper addresses the diagnostic algorithm to be applied in prenatal follow-up depending on the type of PGT performed, the results obtained and the related diagnostic value based on the most recent literature data and Italian and international recommendations.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":"1015-1024"},"PeriodicalIF":3.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11950599/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143051860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Critical appraisal of 'pregnancy outcomes in patients with non-obstructive azoospermia undergoing micro-TESE: comparison of fresh vs. frozen-thawed testicular sperm'.","authors":"Hossam Elzeiny","doi":"10.1007/s10815-025-03419-3","DOIUrl":"10.1007/s10815-025-03419-3","url":null,"abstract":"","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":"1029-1031"},"PeriodicalIF":3.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11950481/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143458029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A review of the association between in vitro fertilization and children's neurocognitive development.","authors":"Nicholas Aderinto, Emmanuel Kokori, Gbolahan Olatunji, Ikponmwosa Jude Ogieuhi, Israel Charles Abraham, Bonaventure Michael Ukoaka, Adetola Emmanuel Babalola, Yewande Abigail Adebayo, Chimezirim Ezeano, Oluwaseun Oyewo, Ganiyat Adekemi Adeshina","doi":"10.1007/s10815-024-03348-7","DOIUrl":"10.1007/s10815-024-03348-7","url":null,"abstract":"<p><p>This review investigated the current research on the association between in vitro fertilization and children's neurocognitive development. Twenty studies were analyzed, encompassing over 23,000 children conceived through IVF, and compared to those conceived naturally. The findings on overall cognitive function were mixed, as measured by IQ. Some studies showed no significant differences between IVF and naturally conceived children, while others suggested slight variations. There is emerging evidence that IVF might correlate with specific cognitive domains like language and motor skills, although more research is needed. Several established factors, including maternal age, education level, and birth weight, are associated with children's cognitive development, regardless of conception method. Future research should explore how these factors interact with IVF and investigate a broader range of cognitive domains. Socioeconomic background and parental involvement are essential considerations for understanding a child's developmental trajectory. The inconclusive nature of some findings highlights the need for further research with larger sample sizes, more extended follow-up periods, and robust methodologies. This research has potential implications for parents considering IVF or ICSI, healthcare professionals providing guidance, and future efforts to tailor support systems for children conceived through assisted conception techniques. Open communication about the current state of knowledge and responsible communication of research findings is crucial.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":"697-706"},"PeriodicalIF":3.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11950484/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142836609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transvaginal ovarian drilling for polycystic ovary syndrome prior to in vitro fertilization dramatically improves embryo yield, implantation, and ongoing pregnancy rates.","authors":"Sarah C Rubin, Moses Bibi, Andrzej Breborowicz, Patricia Chau, Martin D Keltz","doi":"10.1007/s10815-024-03362-9","DOIUrl":"10.1007/s10815-024-03362-9","url":null,"abstract":"<p><strong>Purpose: </strong>To evaluate the effect of transvaginal ovarian drilling (TVOD) on IVF outcomes in subjects with clomiphene-resistant PCOS and a history of IVF failure.</p><p><strong>Methods: </strong>Between 2008 and 2011, 19 subjects with sonographically PCOS and a history of failure to ovulate to high-dose clomiphene citrate were prospectively followed and underwent TVOD at a university hospital-based IVF program.</p><p><strong>Results: </strong>In 15 subjects who underwent 30 fresh paired IVF cycles TVOD resulted in a significantly higher number of oocytes retrieved (7.2 ± 5.9 vs. 13.2 ± 5.9, p = 0.007), mature oocytes retrieved (4.6 ± 3.4 vs. 9.5 ± 5.2, p = 0.002), embryos (3.8 ± 2.7 vs. 8.5 ± 4.5, p = 0.0002), and blastocysts (0.73 ± 1.33 vs. 2.77 ± 2.7, p = 0.037). Among all IVF cycles, 19 subjects underwent 23 fresh IVF cycles prior to TVOD and 21 fresh cycles within 6 months following TVOD lead to higher implantation (0.10 vs. 0.37, p = 0.001), clinical pregnancy (17.4% vs. 61.9%, p = 0.002), and ongoing pregnancy rates (4.4% vs. 47.6%, p = 0.014).</p><p><strong>Conclusion: </strong>In this prospective cohort study, TVOD appears to markedly improve IVF outcomes in subjects with clomiphene resistant PCOS and a history of IVF failure.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":"839-845"},"PeriodicalIF":3.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11950547/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142882171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The impact of spillover and crossover effects of job stressors on pregnancy rates in couples undergoing in vitro fertilization treatments.","authors":"Keren Turgeman Lupo, Ya'arit Bokek-Cohen, Netanella Miller, Einat Haikin Herzberger, Amir Wiser","doi":"10.1007/s10815-024-03373-6","DOIUrl":"10.1007/s10815-024-03373-6","url":null,"abstract":"<p><strong>Purpose: </strong>Studies have shown mixed findings regarding the impact of stress on the success of fertility treatments. To the best of our knowledge, stress in the context of the workplace has not been investigated to date in relation to the success of fertility treatments. This research investigates the impact of work-related stress and emotional exhaustion experienced by both partners on in vitro fertilization (IVF) treatment outcomes.</p><p><strong>Methods: </strong>We conducted a cross-sectional study that included 44 heterosexual couples (N = 88) in which both partners filled out the research questionnaire. The couples were recruited in a hospital IVF unit in the center of Israel.</p><p><strong>Results: </strong>Women's job-related stress and emotional exhaustion lowered their chances of achieving pregnancy when undergoing IVF treatments. Moreover, when partners' emotional exhaustion was relatively low, the job-related stress of women did not affect pregnancy outcomes.</p><p><strong>Conclusion: </strong>This is the first study to explore whether job-related stressors of both partners may have an impact on success rates of IVF treatments. We propose some practical implications as to how to eradicate their negative impact on IVF outcomes.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":"847-853"},"PeriodicalIF":3.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11950490/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142926775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimal embryo management strategies for patients undergoing antagonist protocols in IVF treatment.","authors":"Hao Wei, BaoPing Zhu, LeiYu Deng, MeiFang Zeng, JinLiang Duan","doi":"10.1007/s10815-024-03365-6","DOIUrl":"10.1007/s10815-024-03365-6","url":null,"abstract":"<p><strong>Purpose: </strong>Selection of optimal embryo transfer strategies for IVF patients treated with antagonist protocols.</p><p><strong>Methods: </strong>A retrospective study was conducted to assess whether whole embryo culture to the blastocyst stage could enhance the cumulative live birth rate (CLBR). The study included data from the first oocyte retrieval cycle of 4131 patients who underwent IVF treatment between January 2018 and June 2022. Patients were categorized into two strategies based on whether they underwent partial blastocyst culture (PBC) or whole embryo culture (WEC), and were further subdivided into three subgroups according to the number of high-quality embryos on Day 3: 0-2 embryos (subgroup 1), 3-7 embryos (subgroup 2), and 8 or more embryos (subgroup 3). Propensity score matching was used to perform a 1:1 match for patients across the three subgroups. The primary outcome measure was the CLBR per oocyte retrieval cycle.</p><p><strong>Results: </strong>After propensity score matching, there were no statistically significant differences in the baseline data among patients across the three corresponding subgroups for the PBC and WEC strategies. The analysis of CLBR in single oocyte retrieval cycles revealed that subgroup 1 had a significantly higher rate with the PBC strategy compared to the WEC strategy (33.0% vs. 25.7%, P = 0.018). In subgroups 2 and 3, there were no statistically significant differences in the CLBR between patients using the two embryo management strategies.</p><p><strong>Conclusions: </strong>When patients have 0-2 high-quality embryos on Day 3, opting for Day 3 embryo transfer rather than blastocyst culture can increase the chances of embryo transfer and improve the CLBR.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":"827-838"},"PeriodicalIF":3.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11950445/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142909676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}