Journal of Applied Oral Science最新文献

{"title":"Exploring polymorphisms in genes encoding growth factors associated with non-syndromic cleft lip with or without cleft palate and tooth agenesis.","authors":"Gabriela Fonseca-Souza, Vitória Somma Tessari, Rafaela Scariot, Christian Kirschneck, Ricardo Della Coletta, Erika Calvano Küchler, Juliana Feltrin-Souza","doi":"10.1590/1678-7757-2024-0501","DOIUrl":"10.1590/1678-7757-2024-0501","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the association between non-syndromic cleft lip with or without cleft palate (NSCL±P) and tooth agenesis (TA), as well as the association of both conditions with polymorphisms in genes encoding growth factors.</p><p><strong>Methodology: </strong>This cross-sectional study included children with NSCL±P and a control group of children without NSCL±P. Permanent teeth TA (excluding third molars) was evaluated using panoramic radiographs by a trained examiner. Only TA located outside the cleft was considered in the NSCL±P group. Genetic polymorphisms in Transforming Growth Factor Beta 1 (TGFB1)-rs1800470 and rs4803455-Transforming Growth Factor Beta Receptor 2 (TGFBR2)-rs3087465 and rs764522-Epidermal Growth Factor (EGF)-rs4444903 and rs2237051-and Epidermal Growth Factor Receptor (EGFR)-rs2227983- were genotyped by real-time PCR allele discrimination from buccal cell samples. Associations were tested by uni and multivariable Poisson regression models (5% significance level).</p><p><strong>Results: </strong>A total of 243 children-127 with NSCL±P (mean age = 8.80±2.14 years) and 116 without NSCL±P (mean age = 8.58±2.03 years) were included. TA was more frequent in the NSCL±P group (23.8%) than in the control group (6.2%) (p<0.01). The EGF rs2237051 was significantly associated with NSCL±P, independently of the other variables (PRa=1.41; p=0.042). Regarding TA, only the cleft presence was associated with a higher prevalence of TA regardless of different variables (PRa=3.70; p=0.001). There was no association between TA and the investigated genetic polymorphisms. When TA and NSCL±P were considered together, a borderline association was observed with rs1800470 in TGFB1 (p=0.06).</p><p><strong>Conclusion: </strong>NSCL±P is associated with TA outside the cleft area. The EGF rs2237051 was associated with NSCL±P. Polymorphisms in genes encoding growth factors are not associated with TA.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"33 ","pages":"e20240501"},"PeriodicalIF":2.2,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11978285/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143657328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunomodulatory effects of apical papilla cells on periodontal ligament fibroblasts stimulated with Escherichia coli lipopolysaccharide: an in vitro study.","authors":"Alexandre Guimarães Dos Santos, Karollyne Santos Spigariol, Letícia Martins Santos, Marinella Holzhausen, Carla Renata Sipert","doi":"10.1590/1678-7757-2024-0338","DOIUrl":"10.1590/1678-7757-2024-0338","url":null,"abstract":"<p><strong>Background: </strong>The role of human Stem Cells from the Apical Papilla (SCAP) in tissue regeneration has been described, but their impact on modulating the apical inflammatory process by other surrounding cell populations, such as periodontal ligament fibroblasts (PLFs), is unclear. Therefore, we investigated the role of SCAP in the activation of PLFs in vitro.</p><p><strong>Methods: </strong>Primary SCAP culture was used to obtain conditioned media (CM). A primary human PLF culture was established and stimulated with increasing concentrations of Escherichia coli lipopolysaccharide (LPS) (0.01, 0.1, and 1 µg/mL). At the 24 h time-point, an MTT viability assay was performed, and interleukin (IL)-6 and chemokine (CC-motif) ligand 2 (CCL2) levels were quantified by enzyme-linked immunosorbent assay. Then, PLFs were stimulated with LPS in the presence of SCAP-CM (1:5 dilution) for cell viability assessment and cytokine detection. The following groups were tested: PLF activated with LPS at concentrations of 0.01 and 1 µg/mL with or without SCAP-CM; a group with PLF stimulated by SCAP-CM alone; and a control group (proliferation medium only). The experiments were conducted in triplicate and sextuplicate. Statistical analyses were performed using analysis of variance followed by Tukey's post-hoc test, with statistical significance established at 5% (p=0.05).</p><p><strong>Results: </strong>The MTT assay showed no cytotoxicity of LPS or SCAP-CM on PLFs (p>0.05). The production of CCL2 and IL-6 significantly increased in the presence of SCAP-CM regardless of the presence of LPS (p<0.0001).</p><p><strong>Conclusion: </strong>SCAP-CM significantly enhanced the release of proinflammatory cytokines by PLFs in vitro.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"33 ","pages":"e20240338"},"PeriodicalIF":2.2,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11869941/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143657308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lipid nanocarrier containing eugenol for denture hygiene: evaluation of efficacy against Candida biofilms.","authors":"Irisvaldo Lima Guedes, Matheus Oliveira do Nascimento, Leandro de Sousa Dias, Alyne Rodrigues de Araujo-Nobre, Humberto Medeiros Barreto, Érika de Araújo Abi-Chacra, Ana Cristina Vasconcelos Fialho, Gláuber Campos Vale, André Luis Menezes Carvalho","doi":"10.1590/1678-7757-2024-0455","DOIUrl":"10.1590/1678-7757-2024-0455","url":null,"abstract":"<p><strong>Background: </strong>This article is derived from Irisvaldo Lima Guedes's Master's dissertation and is available at the address: https://sigaa.ufpi.br/sigaa/public/programa/noticias_desc.jsf?lc=pt_BR&id=370&noticia=519307121 Eugenol has demonstrated efficacy against Candida spp., which is highly prevalent in denture wearers. However, the low water solubility and high volatility limit its application. The encapsulation in nanostructured lipid carriers (NLCs) may be a viable approach for developing new sanitizing agents for denture hygiene.</p><p><strong>Objective: </strong>To develop a sanitizing dispersion for denture hygiene using nanostructured lipid carriers (NLCs) containing eugenol and to evaluate the efficacy against Candida spp. biofilms.</p><p><strong>Methodology: </strong>The formulation was prepared using the ultrasonication method and characterized in terms of particle size (PS), polydispersity index (PDI), zeta potential (ZP), and encapsulation efficiency (EE). The minimum inhibitory concentration (MIC) was determined by the broth microdilution method and the antifungal activity was evaluated by four treatment groups (nanostructured formulation containing eugenol (NFE), free eugenol (FE), saline solution (SS), and the drug-free formulation NFW after eight hours of immersion in biofilms of two Candida species (Candida albicans and Candida glabrata) adhered to polymethyl methacrylate resin specimens.</p><p><strong>Results: </strong>The nanoparticles of NFE showed a particle size of 199.5±2.55 nanometers (nm) as measured by DLS, high homogeneity (0.07±0.02), an EE of 83.07±0.23, and a negative ZP (-25.86±0.65). The MICs of FE for Candida albicans and Candida glabrata were up to 10 times (64 µg/mL) and eight times (128 µg/mL) higher, respectively, than the MICs of NFE (6 µg/mL and 16 µg/mL). The biofilms of these microorganisms showed a significant reduction after immersion in NFE compared to the other tested groups (FE, NBF, and SS) (P<0.0001).</p><p><strong>Conclusion: </strong>The NFE demonstrated fungicidal activity against the isolated strains and significantly reduced Candida biofilms, thus showing promising performance for the sanitization of dentures over eight hours.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"33 ","pages":"e20240455"},"PeriodicalIF":2.2,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12002739/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143639456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bmal1 knockout aggravates Porphyromonas gingivalis-induced periodontitis by activating the NF-κB pathway.","authors":"Ye Tian, Xinran Liu, Qiuyu Lu, Jiaxin Li, Tianqi Wang, Mei Tian, Yan Ding, Jinle Li","doi":"10.1590/1678-7757-2024-0388","DOIUrl":"10.1590/1678-7757-2024-0388","url":null,"abstract":"<p><strong>Background: </strong>Circadian rhythm disorders and NF-κB are closely linked and can exacerbate periodontitis. However, the mechanisms via which circadian rhythm-related genes influence periodontitis are not yet fully understood.</p><p><strong>Objective: </strong>We investigated the effect of brain and muscle Arnt-like protein-1 (BMAL1) on the NF-κB pathway and downstream inflammatory factors on periodontitis. In this study, Bmal1 homozygous knockout and periodontitis mouse models were established.</p><p><strong>Methodology: </strong>Bone marrow-derived macrophages (BMDMs) from Bmal1-/- mice were cultured and stimulated with lipopolysaccharides. Bone resorption was detected using micro-computed tomography and histological analyses. Gene and cytokine expression was assessed using quantitative reverse-transcription PCR and ELISA. The nuclear translocation of p65 was detected using immunofluorescence.</p><p><strong>Results: </strong>Our findings indicate that Bmal1 knockout exacerbates periodontitis severity in mice by activating the NF-κB signaling pathway with increased nuclear translocation of p65 (p<0.05), as well as increased expression of Il-1b, Il-6, and Tnfα (p<0.01), along with decreased Nr1d1 expression (p<0.05) in BMDMs under inflammation.</p><p><strong>Conclusion: </strong>The results highlight the protective role of Bmal1 in periodontitis and suggest its potential link to the circadian clock's influence on the disease.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"33 ","pages":"e20240388"},"PeriodicalIF":2.2,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11869942/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143501241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ERRATUM.","authors":"","doi":"10.1590/1678-7757-2024er002","DOIUrl":"10.1590/1678-7757-2024er002","url":null,"abstract":"<p><p>[This corrects the article doi: 10.1590/1678-7757-2024-0245].</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"33 ","pages":"e2024er002"},"PeriodicalIF":2.2,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11869940/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143501243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic anti-cancer effects of metformin and cisplatin on YD-9 oral squamous carcinoma cells via AMPK pathway.","authors":"Paras Man Pradhan, Young-Hee Lee, Sungil Jang, Ho-Keun Yi","doi":"10.1590/1678-7757-2024-0385","DOIUrl":"10.1590/1678-7757-2024-0385","url":null,"abstract":"<p><strong>Objective: </strong>This study evaluated whether hypoglycemic drug metformin enhances the anti-cancer effects of cisplatin in YD-9 cells.</p><p><strong>Methodology: </strong>YD-9 cells, derived from oral mucosal squamous cell carcinoma of oral mucosa, were used to assess the combined effects of metformin and cisplatin by means of MTT assay, live and dead cell staining, and colony formation assays to evaluate cell viability and proliferation. Reactive oxygen species level was measured using a Muse cell analyzer. Apoptosis, epithelial-mesenchymal transition, and related molecular pathways were analyzed by western blot. Wound healing assays and Transwell migration assays examined cell migration, whereas monophosphate-activated protein kinase inhibitor Compound C, was utilized to investigate the AMPK pathway.</p><p><strong>Results: </strong>Sequential treatment of YD-9 cells with metformin and cisplatin resulted in decreased cell viability and proliferation, increased ROS levels, and elevated apoptosis compared with the individual drugs. Moreover, the treatment inhibited EMT, wound healing, and cell migration. These results correlated with increased AMPK phosphorylation, a key regulator of cellular energy homeostasis. Introduction of Compound C pre-treatment upregulated N-cadherin and α-smooth muscle actin along with enhanced cell migration.</p><p><strong>Conclusion: </strong>This study found synergism in anti-cancer effects between metformin and cisplatin. Additionally, introduction of Compound C confirmed that EMT inhibition is AMPK dependent. These findings indicate the potential use of metformin as an adjunct drug in anti-cancer treatments, warranting further investigation.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"33 ","pages":"e20240385"},"PeriodicalIF":2.2,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143501246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effects of Stemregen® host modulation therapy on experimentally induced apical periodontitis in rats.","authors":"Fatma Gönüllü, Mevlüt Sinan Ocak, Serkan Dundar, İbrahim Hanifi Özercan","doi":"10.1590/1678-7757-2024-0446","DOIUrl":"10.1590/1678-7757-2024-0446","url":null,"abstract":"<p><strong>Objective: </strong>This study evaluated the effect of Stemregen® nutritional supplement on inflammation and resorption in apical periodontitis using a rat model.</p><p><strong>Methodology: </strong>Rats were divided in three groups: negative control (n=7), positive control (n=10), and Stemregen® (Stem) (n=10). Apical periodontitis was induced in the positive control and Stem groups, and all rats were sacrificed on the 30th day. Serum phosphorus (P), calcium (Ca), and alkaline phosphatase (ALP) were analyzed. Histopathological assessments measured osteoblastic and osteoclastic activity, inflammation, fibrosis, and abscess density. Immunohistochemical analyses evaluated RANKL, TRAP, and OPG levels.</p><p><strong>Results: </strong>Results showed significantly lower osteoblastic activity in the negative control compared to Stem and positive control groups (p=0.005). Osteoclastic activity was higher in the positive control (p=0.032). Inflammation and abscess formation were reduced in the Stem group compared to the positive control (p<0.001). OPG levels were lower in the negative control compared to the other groups (p=0.005).</p><p><strong>Conclusion: </strong>Stemregen® effectively reduced inflammation and bone destruction, suggesting potential benefits for apical periodontitis management, though further research is needed.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"33 ","pages":"e20240446"},"PeriodicalIF":2.2,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11816948/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143189523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbial signatures in head and neck squamous cell carcinoma: an in silico study.","authors":"Loganathan Kavitha, Manogaran Kuzhalmozhi, Jayaseelan Vijayashree Priyadharsini, Arunachalam Arun Kumar, Krishna Mohan Rao Umadevi, Kannan Ranganathan","doi":"10.1590/1678-7757-2024-0392","DOIUrl":"10.1590/1678-7757-2024-0392","url":null,"abstract":"<p><strong>Objectives: </strong>The oral cavity harbors a plethora of bacterial species. Dysbiosis of oral and gut microbiota is associated with several oral and systemic pathologies, such as cancer, obesity, diabetes, atherosclerosis and gastrointestinal diseases. Imbalance in the oral-gut microbial axis has been associated with head and neck squamous cell carcinoma (HNSCC). This study aims to analyze the bacterial profile of HNSCC across various taxonomic units, investigate molecular patterns associated with prevalent bacterial phylum in HNSCC, and compare the bacterial profile in HNSCC and gastrointestinal (GI) carcinoma using computational analysis.</p><p><strong>Methodology: </strong>The microbe-host transcriptomic, proteomic, and epigenetic analyses of HNSCC and GI carcinomas were performed using The Cancer Microbiome Atlas (TCMA) database. The differential expression of the host's mRNA transcripts and proteins associated with tumor microbiome were analyzed using The University of Alabama at Birmingham Cancer data analysis (UALCAN) and Clinical Proteomic Tumor Analysis Consortium (CPTAC) websites.</p><p><strong>Results: </strong>A decrease in Actinobacteria and an enrichment of Flavobacteria at the class level, Neisseriales, Pasteurellales, and Campylobacterales at the order level, Pasteurellaceae, Flavobacteriaceae, Campylobacteraceae, and Peptoniphilaceae at the family level, and Hemophilus, Porphyromonas, and Leptotrichia at the genus level were observed in HNSCC compared to the normal mucosa. RICTOR protein, mRNA transcripts (HIST1H2BB, SCARNA11, TBC1D21 gene), and hsa-miR-200a-5p miRNA were significantly correlated with prevalent bacterial species in HNSCC. A major increase in Actinobacteria, Fusobacteria, and Spirochaetes was observed in HNSCC compared to GI carcinoma.</p><p><strong>Conclusion: </strong>The oral-gut microbial dysbiosis, as reflected by the differential abundance of bacterial species in oral and GI carcinomas, suggests the implication of tumor microbiome and their genomic interactions with the host in carcinogenesis.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"33 ","pages":"e20240392"},"PeriodicalIF":2.2,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11816647/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143189521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study of the role of transmembrane emp24 domain-containing protein 2 in oral squamous cell carcinoma.","authors":"Ruan Zhao-Wei, Jiao Xue-Feng, Xiao Gao-Tian, Chen Jin-Ling, L I Jun, L V Shu-Ying","doi":"10.1590/1678-7757-2024-0305","DOIUrl":"10.1590/1678-7757-2024-0305","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to investigate the role of transmembrane emp24 domain-containing protein 2 (TMED2) in oral squamous cell carcinoma (OSCC).</p><p><strong>Methodology: </strong>A bioinformatics analysis was first conducted to explore TMED2 expression in OSCC and its relation with overall survival. The analysis results were further verified by assessing TMED2 expression levels in human normal oral keratinocyte cells and human OSCC cell lines using quantitative real-time polymerase chain reaction and the Western blot. Finally, the effects of TMED2 knockdown and overexpression on the expression levels of TMED2, ADP-ribosylation factor 1, extracellular signal-regulated kinase ½, and phospho-extracellular signal-regulated kinase ½ proteins were examined in cells using the Western blot.</p><p><strong>Results: </strong>The GEPIA2 database showed that OSCC tissues expressed more TMED2 than normal tissues. At the cellular level, TMED2 expression significantly increased in SCC-4, HSC-3, and CAL-27 cells than in human normal oral keratinocyte cells. TMED2 knockdown reduced cell proliferation, increased the apoptosis rate in SCC-4 cells, and led to a higher proportion of cells in the G0/G1 phase and a lower proportion in the S phase.</p><p><strong>Conclusion: </strong>TMED2 may promote OSCC cell proliferation and inhibit apoptosis, potentially by activation of the ADP-ribosylation factor 1/ extracellular signal-regulated kinase ½ signaling pathway.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"33 ","pages":"e20240305"},"PeriodicalIF":2.2,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11816950/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143065844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of ozone oil and non-surgical periodontal treatment in patients with type 2 diabetes. In-vivo and in-vitro studies with fibroblasts and Candida albicans.","authors":"Raquel Alves do Carmo, Ana Carolina Organista Cörner, Eduardo Ferreira Martins, Gabriel Ouverney, Julio Cesar Thurler Júnior, Bruno Kaufmann Robbs, Vinicius D'Avila Bitencourt Pascoal, Elisa Esposito, Luciane Portas Capelo, Gabriela Alessandra da Cruz Galhardo Camargo","doi":"10.1590/1678-7757-2024-0080","DOIUrl":"10.1590/1678-7757-2024-0080","url":null,"abstract":"<p><strong>Aim: </strong>To evaluate the clinical effectiveness of ozonated sunflower oil (Oz) as an adjunctive of non-surgical periodontal therapy in patients with type 2 diabetes mellitus (DM2), on fibroblast cell viability and migration and the effectiveness of Oz on a Candida albicans (C. albicans) culture.</p><p><strong>Methodology: </strong>In total, 32 sites in 16 DM2 with moderate to advanced periodontal disease with periodontal pocket depths ≥5mm were selected. The treatments were divided into two groups: control, saline solution (SS) as an adjunctive of scaling and root planing (SRP+SS), and test, Oz as an adjunctive of SRP (SRP+Oz). Hematological [fasting glucose level (FGL) and hemoglobin A1c (HbA1c)] and microbiological samples were collected from the participants at baseline and three months after periodontal treatment and the microbiological samples were analyzed by PCR. C. albicans was previously tested by the agar diffusion test. The effect of Oz was tested on cell viability and fibroblast migration.</p><p><strong>Results: </strong>The groups showed no statistically significant differences (paired t-test-p>0.05) regarding hematological parameters, FGL (median - baseline 171.41, 3 months 164mg/dL), and HbA1c (baseline 8%, 3 months 7.5%) (Kruskal-Wallis One-Way Nonparametric-p>0.05) after periodontal therapy. The groups showed statistical differences for periodontal parameters between baseline and three months (paired t-test-p<0.05). PCR analysis showed a reduction in the percentage of C. albicans in the SRP+Oz group after three months (McNemar's test-p=0.002). Cell viability was lower in the high glucose Dulbecco's modified Eagle's medium (4500 mg/L) than in low glucose (1000 mg/L) (RM-ANOVA-p<0.0001). The wound healing test showed reduced fibroblast migration (one-way ANOVA with Dunnett's post-test-p<0.01). Oz showed high C. albicans antifungal inhibition (Kruskal-Wallis test-p=0.0001).</p><p><strong>Conclusions: </strong>SRP+Oz effectively reduced C. albicans in-vitro and in-vivo but showed no clinical improvements compared to the control. Cell viability and wound healing of fibroblasts showed no improvements.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"33 ","pages":"e20240080"},"PeriodicalIF":2.2,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11805463/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143023484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}