Journal of Applied Oral Science最新文献

{"title":"Effects of highly leukotoxic Aggregatibacter actinomycetemcomitans associated with ligature-induced periodontitis on oral and gut tissues in male rats.","authors":"Gabriela Furian Trama Ferraz, Camila Philipe de Araujo Camilo, Ana Luisa Palhares de Miranda, Mariana Alves Soares, Carmelo Sansone, Ricardo Tadeu Lopes, Aline Correa Abrahão, Ana Paula Vieira Colombo, Carina Maciel Silva-Boghossian","doi":"10.1590/1678-7765-2025-0731","DOIUrl":"10.1590/1678-7765-2025-0731","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the effects of gastric administration of the highly leukotoxic Aggregatibacter actinomycetemcomitans JP2 strain (Aa JP2), with or without ligature-induced periodontitis, on periodontal and small intestinal tissues in a rat model.</p><p><strong>Methodology: </strong>Forty-eight-week-old male Wistar rats were divided into four groups (n=10/group): Control (CG), Periodontitis (PG), Periodontitis and Aa JP2 (PAaG), and Aa JP2 only (AaG). Ligatures were placed in PG and PAaG on day one and maintained for 42 days. After six weeks of twice-weekly gastric Aa JP2 inoculation, mandibles and small intestines were analyzed using stereomicroscopy, micro-computed tomography (CT), and histopathology to assess alveolar bone loss, trabecular architecture, inflammatory infiltrate, vascular congestion, goblet cell density, and villus morphology. Statistical analyses included the Kruskal-Wallis, Mann-Whitney, and Chi-square tests for group comparisons (p<0.05), as well as Spearman's correlation test.</p><p><strong>Results: </strong>PG and PAaG exhibited significantly greater bone loss compared to CG and AaG (p<0.05). Micro-CT analysis revealed reduced trabecular thickness in AaG (342.4±23.1µm) compared to PG (378.2±28.1µm) and PAaG (385.5±45.1µm) (p<0.05). PAaG and AaG presented higher inflammatory infiltrate scores (>751 inflammatory cells) compared to CG and/or PG (p<0.05). Elevated vascular congestion and/or goblet cell hyperplasia in the jejunum and ileum were observed in PAaG and AaG compared to CG and PG (p<0.05). Villus height (duodenum/ileum) and villus width (jejunum/ileum) differed significantly among groups (p<0.0001). Significant correlations were identified: the alveolar bone volume/total volume ratio was positively associated with duodenal vascular congestion (rho=0.738; p=0.037); the periodontal inflammatory infiltrate was positively associated with jejunal goblet cell counts (rho=0.704; p=0.007); alveolar bone loss was positively associated with both duodenal and jejunal villus height (rho≥0.762; p<0.05); and bone volume was negatively associated with both jejunal vascular congestion (rho=-0.696; p=0.003) and jejunal goblet cell counts (rho=-0.617; p=0.011).</p><p><strong>Conclusions: </strong>Gastric inoculation with Aa JP2 exacerbates ligature-induced periodontitis and independently induces intestinal morphological changes.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"34 ","pages":"e20250731"},"PeriodicalIF":3.0,"publicationDate":"2026-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13123778/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147433124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hydrogel microarchitecture as a regulatory cue for in vitro odontogenic differentiation.","authors":"Letícia Alves Martins de Carvalho, Vitor de Toledo Stuani, Isabela Sanches Pompeo da Silva, Thayná Souza Berteli, Nicoly Gabriely Gonçalves, Diana Gabriela Soares, Ester Alves Ferreira Bordini","doi":"10.1590/1678-7765-2025-0607","DOIUrl":"10.1590/1678-7765-2025-0607","url":null,"abstract":"<p><strong>Objective: </strong>This study introduces an innovative, cost-effective, and easily reproducible strategy for engineering three-dimensional bioprinted GelMA-based scaffolds, designed with ordered macroporous and tubular architectures, and integrated microfluidic channels to advance structural and functional performance. Their geometric features were specifically designed to investigate how microarchitectural cues influence the mineralizing cell differentiation of human dental pulp cells (HDPCs).</p><p><strong>Methodology: </strong>The scaffolds were fabricated via an indirect bioprinting process using resin molds, resulting in cylindrical structures with distinct grid or honeycomb surface architectures. Biomaterials were characterized for morphology, surface topography, porosity, pore diameter, and degradability. Biological performance was evaluated by culturing HDPCs for 21 days to assess viability, proliferation, and mineralizing differentiation (ANOVA/Tukey; α=0.05).</p><p><strong>Results: </strong>Both scaffold designs exhibited interconnected porous networks, with the honeycomb configuration presenting significantly larger pores. HDPCs cultured on the scaffolds showed high viability and proliferation, with the honeycomb architecture promoting elevated ALP activity. However, the grid architecture more effectively influenced odontoblastic differentiation and mineralized matrix deposition.</p><p><strong>Conclusion: </strong>Our findings highlight the impact of biomaterial architecture on cellular behavior and reveal the potential of this novel bioprinting approach for bioactive dentin regeneration in dental tissue engineering.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"34 ","pages":"e20250607"},"PeriodicalIF":3.0,"publicationDate":"2026-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13123770/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147433075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of Stage I-II periodontitis on the mechanical, structural, and biological properties of leukocyte and platelet-rich fibrin.","authors":"Sarvar Ibrahimli, Meltem Karsiyaka Hendek, Kubilay Baris, Ebru Olgun","doi":"10.1590/1678-7765-2025-0483","DOIUrl":"10.1590/1678-7765-2025-0483","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to compare the mechanical, biological, and structural properties of a leukocyte- and platelet-rich fibrin (L-PRF) membrane in periodontally healthy patients and those with periodontitis. A total of 30 individuals (17 men and 13 women) with a median age of 41 years were divided into two groups as periodontally healthy (control group) and as having periodontitis (test group).</p><p><strong>Methodology: </strong>Blood samples were collected and centrifuged at 2700 rpm for 12 minutes. A tensile test was performed to determine the mechanical properties of L-PRF membranes. Elasticity-modulus, tensile strength, and stretch at rupture were calculated. Platelet and leucocyte counts and ratios were calculated. Fibril thickness and density were evaluated by scanning electron microscopy imaging. The comparisons between groups were analyzed by the Mann-Whitney U test, and a p<0.05 value was considered statistically significant.</p><p><strong>Results: </strong>In the comparison of the control group versus the test group, pocket depth (3.55 (1.59-3.97) vs. 1.52 (1.47-3.53)), clinical attachment level (3.11 (1.77-4.23) vs. 0.06 (0.05-3.68)), plaque index (1.63 (0.05-1.75) vs. 0.04 (0.04-1.58)), gingival index (1.55 (0.04-1.63) vs. 0.05 (0.04-1.49)), and bleeding on probing (56.25 (3.7-70.11) vs. 3.57 (2.67-47.12)) were statistically significantly higher in the test group than in the control group (p= 0.009, p=0.008, p=0.008, p=0.009, p=0.009, respectively). Elasticity-modulus, tensile strength, and stretch at rupture values showed no statistically significantly differences between groups (p=0.745, p=0.754, p=0.602, respectively). Fibril thickness and density values showed no statistically significant differences between groups (p=1.000, p=0.753, respectively). Platelet count and platelet and leukocyte ratio values showed no statistically significant differences between groups (p=0.754, p=0.600, p=0.142, respectively). Leukocyte count was found to be statistically significantly higher in the control group than in the test group (p=0.028).</p><p><strong>Conclusion: </strong>This study showed no effect of Stage I-II periodontitis on the mechanical, structural, and biological properties of L-PRF. Further studies with larger sample sizes are required to confirm these findings.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"34 ","pages":"e20250483"},"PeriodicalIF":3.0,"publicationDate":"2026-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13123785/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147433038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of live and heat-killed Bifidobacterium lactis in rats with induced periodontitis.","authors":"Tainá da Silva Tricoly, Thais Aguiar Santos, Camila Lopes Ferreira, Itza Amarilis Ribeiro Pinto, Victoria Clara da Silva Lima, Camilla Magnoni Moretto Nunes, Ana Lia Anbinder, Maria Aparecida Neves Jardini","doi":"10.1590/1678-7765-2025-0634","DOIUrl":"10.1590/1678-7765-2025-0634","url":null,"abstract":"<p><strong>Background: </strong>Probiotics and postbiotics have emerged as promising adjunctive therapies in managing periodontal disease. Bifidobacterium animalis subsp. lactis HN019 has shown antimicrobial and immunomodulatory effects in both experimental and clinical settings when administered orally. However, the systemic impact of this strain, independent of local oral effects, remains unclear.</p><p><strong>Objective: </strong>To evaluate the systemic effects of live and heat-killed B. animalis subsp. lactis HN019 in a rat model of ligature-induced periodontitis, excluding direct contact with the oral cavity.</p><p><strong>Methodology: </strong>A total of 32 Wistar rats were randomly assigned to four groups (n=8): control (C), periodontitis only (EP), periodontitis + probiotic (PRO), and periodontitis + postbiotic (POS). Periodontitis was induced by placing a cotton ligature around the cervical region of the lower right first molar, inserted into the gingival sulcus. Treatments were administered via oral gavage for 30 days before and 15 days after periodontitis induction. Alveolar bone loss and periodontal parameters were assessed using micro-computed tomography (microCT) and histomorphometric analysis.</p><p><strong>Results: </strong>MicroCT revealed that ligature effectively induced periodontitis, reducing BV/TV and Tb.N and increasing Tb.Sp and Po.Tot. Probiotic and postbiotic treatments did not improve outcomes. Bone loss was lowest in the control group, with no differences between EP, PRO, and POS.</p><p><strong>Conclusion: </strong>Systemic administration of Bifidobacterium animalis subsp. lactis HN019 or its derived postbiotic resulted in no significant improvements in periodontal outcomes in this experimental model. Further investigations using integrative approaches are needed to better characterize the systemic effects of probiotics and postbiotics.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"34 ","pages":"e20250634"},"PeriodicalIF":3.0,"publicationDate":"2026-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13123780/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147433090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of chitosan addition to at-home bleaching gel with and without calcium on the physical properties of tooth enamel.","authors":"Laura Nobre Ferraz, Letícia de Souza Franco, Carolina Meneghin Barbosa, Marcos Roberto de Lima Benati, Tatiane Cristina Dotta, Waldemir Francisco Vieira-Junior, Débora Alves Nunes Leite Lima","doi":"10.1590/1678-7765-2025-0513","DOIUrl":"10.1590/1678-7765-2025-0513","url":null,"abstract":"<p><p>Dental bleaching is a widely used esthetic treatment, but concerns persist regarding its potential effects on enamel integrity. The incorporation of remineralizing and protective agents into bleaching gels, such as calcium and chitosan, has been proposed to mitigate these effects.</p><p><strong>Objective: </strong>This in vitro study evaluated the effects of adding 2% chitosan to at-home bleaching gels with and without calcium on enamel physical properties.</p><p><strong>Methodology: </strong>Bovine enamel specimens stained with black tea were assigned to five groups (n = 12): control (no bleaching), 7.5% hydrogen peroxide (7.5% HP), 7.5% HP + 2% chitosan (7.5% HP+ Chi), 7.5% HP + calcium (7.5% HP + Ca), and 7.5% HP + calcium + chitosan (7.5% HP + Ca + Chi). Bleaching was performed for 14 days (1 hour/day). Color (∆L*, ∆a*, ∆b*, ∆Eab, ∆E00, and ∆WID), surface roughness, and surface microhardness (SMH) were evaluated at baseline (T1) and after treatment (T2). Mineral content (Ca, P, and the Ca/P ratio) was assessed by energy-dispersive X-ray spectroscopy and enamel morphology by scanning electron microscopy. Statistical analyses included the Kruskal-Wallis and Dunn's tests, mixed models, and ANOVA.</p><p><strong>Results: </strong>Groups containing chitosan (7.5% HP + Chi and 7.5% HP + Ca + Chi) showed the lowest surface roughness and the highest microhardness values. For these groups, SMH showed no decrease after treatment (T2), remaining similar to the initial values (T1). Color changes were similar among all bleached groups. Calcium, phosphorus, and Ca/P levels were the same for all groups.</p><p><strong>Conclusion: </strong>Adding 2% chitosan to 7.5% hydrogen peroxide-based at-home bleaching gels appears to be a promising approach to protect enamel against changes in roughness, microhardness, and mineral content without compromising bleaching efficacy.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"34 ","pages":"e20250513"},"PeriodicalIF":3.0,"publicationDate":"2026-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13123782/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147354926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative analysis of connective tissue graft versus ozone-enriched platelet-rich fibrin in the treatment of Cairo class I gingival recessions: a randomized, double-blind, controlled clinical trial.","authors":"Marina Pereira Silva, Maria Ritha Veiga Colognese, Carlos Augusto Nassar, Veridiana Camilotti","doi":"10.1590/1678-7765-2025-0565","DOIUrl":"10.1590/1678-7765-2025-0565","url":null,"abstract":"<p><p>Gingival recession is characterized by the apical displacement of the marginal gingiva, exposing the root surface and causing aesthetic and functional issues such as dentin hypersensitivity and cervical lesions. Subepithelial connective tissue grafts (SCTG) are considered the gold standard for root coverage. Recently, ozone-enriched advanced platelet-rich fibrin (A-PRF) has emerged as a promising alternative due to its regenerative potential and biocompatibility.</p><p><strong>Objectives: </strong>To compare the clinical efficacy of ozone-enriched A-PRF and SCTG combined with the coronally advanced flap (CAF) technique to treat Cairo Class I gingival recessions.</p><p><strong>Methodology: </strong>This randomized, double-blind, controlled clinical trial included 22 healthy patients (mean age 32.23±11.86 years, range 20-60 years), each with bilateral Cairo Class I recessions. Sites were treated with either CAF + SCTG (control) or CAF + ozone-enriched A-PRF (test). Clinical parameters were assessed at baseline, 90, and 180 days. Data were analyzed using the Shapiro-Wilk, repeated measures ANOVA, Tukey, and Friedman tests (α=0.05) on GraphPad Prism 8.0®. This study adhered to the CONSORT guidelines for randomized clinical trials.</p><p><strong>Results: </strong>Complete root coverage (100%) was achieved in 13 of 22 sites (60%) in the SCTG group and in nine of 22 cases (43%) in the ozone-enriched A-PRF group. Both groups statistically and significantly improve over time in selected clinical parameters (p<0.05). The SCTG group showed significant gains in all evaluated outcomes, whereas the ozone-enriched A-PRF group showed significant improvements in clinical attachment level, recession height, recession width, and dentin hypersensitivity. SCTG more greatly increased gingival thickness (0.77±0.52 mm) and keratinized tissue width (0.81±0.95 mm) than ozone-enriched A-PRF (0.27±0.55 mm and 0.22±0.42 mm, respectively).</p><p><strong>Conclusions: </strong>Both treatment approaches effectively managed Cairo Class I gingival recessions. SCTG promoted superior soft tissue augmentation, whereas ozone-enriched A-PRF achieved comparable reductions in recession-related parameters. The limitations of this study include its short follow-up period and the absence of histological assessment. Clinical Trial Register: RBR-537bzqt.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"34 ","pages":"e20250565"},"PeriodicalIF":3.0,"publicationDate":"2026-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13123794/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147354955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Saliva liquid biopsy: MMP2, MMP9, and TIMP2 as potential diagnostic biomarkers in oral squamous cell carcinoma. An observational case-control study.","authors":"Sergio D Cruz-Romero, Ana Margarita Baldión, José Antonio Hakim Tawil, Alberto Escallon, Paula A Rodriguez-Urrego, Paula Alejandra Baldión","doi":"10.1590/1678-7765-2025-0555","DOIUrl":"10.1590/1678-7765-2025-0555","url":null,"abstract":"<p><p>Oral squamous cell carcinoma (OSCC) is the most common malignant neoplasm in the oral cavity, characterized by high morbidity and mortality. Various proteins, including matrix metalloproteinases (MMPs), have been investigated as potential biomarkers for diagnosing and prognosing this disease.</p><p><strong>Objective: </strong>To evaluate the utility of MMP-2, MMP-9, and the tissue inhibitor of metalloproteinase-2 (TIMP-2) as biomarkers in diagnosing and prognosing OSCC.</p><p><strong>Methodology: </strong>This retrospective case-control study involved 60 cases and 30 controls from the University Hospital Fundación Santa Fe de Bogotá, part of the Colombian cohort of the InterChange/Headspace study. Saliva samples were collected using a 60-second mouth rinse with 10 mL of sterile saline solution, along with serum and oral tissue samples and clinical and sociodemographic data. MMP-9, MMP-2, and TIMP-2 concentrations in saliva were determined using ELISA, whereas tissue samples were analyzed by immunohistochemistry. MMP activity in saliva was quantified using a generic activity assay.</p><p><strong>Results: </strong>Salivary MMP-9 and MMP-2 concentrations were significantly higher in cases than in controls, whereas no significant differences were observed for TIMP-2. ROC curve analysis showed excellent discriminatory capacity for salivary MMP-9 (AUC=0.946; sensitivity=0.817; specificity=0.867), good performance for MMP-2 (AUC=0.708; sensitivity=0.717; specificity=0.633), and lower discriminatory ability for TIMP-2 (AUC=0.630; sensitivity = 0.617; specificity=0.500). Elevated MMP-9 concentrations were observed in 33% of the serological samples. The immunohistochemistry of MMP-2, MMP-9, and TIMP-2 showed differences between cases and controls.</p><p><strong>Conclusions: </strong>This study highlights salivary MMP-9 and MMP-2, together with MMP activity, as biomarkers of interest for OSCC that may contribute to the understanding of disease-related molecular changes detectable by liquid biopsy. While elevated salivary levels were observed in OSCC cases when compared to controls, these findings should be interpreted as exploratory. Elevated serological MMP-9 levels were observed in a small subset of cases. However, the limited sample size and the absence of a control group in this study preclude diagnostic interpretation. Larger, well-powered, and externally validated studies are required to determine the potential clinical utility of these biomarkers.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"34 ","pages":"e20250555"},"PeriodicalIF":3.0,"publicationDate":"2026-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13123767/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147355120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bond strength in class V cavities: an in vitro study on beveling, universal adhesive strategy, and composite type.","authors":"Felipe José Ribeiro de Melo, Viviane Rocha, Vivian Leite Martins, Marcos Vinícius Salvador, Adriano Fonseca Lima, Andrea Nóbrega Cavalcanti","doi":"10.1590/1678-7765-2025-0608","DOIUrl":"10.1590/1678-7765-2025-0608","url":null,"abstract":"<p><p>Cervical Class V lesions present restorative challenges due to their multifactorial etiology and complex bonding substrates.</p><p><strong>Objectives: </strong>This study evaluated the influence of marginal bevel, bonding using a universal adhesive (etch-and-rinse or self-etch), and the resin composite type (conventional or bulk-fill) on the bond strength of simulated Class V cavities.</p><p><strong>Methodology: </strong>A total of 80 dental fragments (7mm×7 mm×2 mm) were obtained from bovine incisors. Cylindrical cavities with enamel margins and internal dentin walls were prepared centrally in each specimen. Samples were randomly assigned to groups based on three factors: presence or absence of bevel, bonding strategy (etch-and-rinse or self-etch), and resin composite type (conventional or bulk-fill). Groups with bevel received a 1 mm enamel bevel along the preparation margins using a conical diamond bur. Adhesive and restorative procedures were performed following the manufacturer's recommendations. Bond strength was assessed using a push-out test on a universal testing machine. Data were analyzed using three-way ANOVA and Tukey's post-hoc test (α=5%).</p><p><strong>Results: </strong>Presence of a marginal bevel did not significantly influence bond strength. The etch-and-rinse bonding strategy resulted in significantly higher bond strength than the self-etch approach, and bulk-fill composites presented superior performance compared with conventional composites.</p><p><strong>Conclusions: </strong>Combining a universal adhesive applied with the etch-and-rinse strategy and a bulk-fill composite proved to be an effective approach for enhancing bond strength with enamel and dentin substrates. Beveling of enamel margins did not improve the bond strength of Class V restorations, suggesting that this procedure may be unnecessary when margins are in enamel. Avoiding beveling in such situations contributes to preserve sound tooth structure and supports a more conservative restorative approach.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"34 ","pages":"e20250608"},"PeriodicalIF":3.0,"publicationDate":"2026-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13123773/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147354939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated analysis identifies CCNA2 as a candidate diagnostic and prognostic biomarker in oral tongue squamous cell carcinoma.","authors":"Ceren Gumedag, Sevcan Atay","doi":"10.1590/1678-7765-2025-0618","DOIUrl":"10.1590/1678-7765-2025-0618","url":null,"abstract":"<p><strong>Background: </strong>Oral tongue squamous cell carcinoma (OTSCC) is an aggressive malignancy with poor prognosis, necessitating reliable biomarkers.</p><p><strong>Methodology: </strong>Genes with significantly higher expression in OTSCC tumor tissues compared to normal tongue tissues were identified via integrated transcriptomic analysis of seven GEO datasets. To assess their diagnostic and prognostic potential, these genes were further characterized using multi-omic and clinical data from the TCGA-OTSCC and CPTAC-OTSCC cohorts.</p><p><strong>Results: </strong>A total of 1,117 genes were found to be upregulated in OTSCC tissues, among which only CCNA2 (Cyclin A2) was significantly associated with both reduced overall survival (OS) and disease-free survival (DFS) in the TCGA-OTSCC cohort (n=128), based on Cox proportional hazards regression and Kaplan-Meier analyses. CCNA2 showed moderate prognostic performance (AUC=0.63 for OS; AUC=0.65 for DFS) and was significantly upregulated in higher-grade tumors (p=0.01) and in deceased patients (p=0.03). No somatic mutations or promoter methylation alterations were observed in CCNA2 based on TCGA data. In CPTAC-OTSCC samples (n=18), CCNA2 protein expression was significantly higher in tumor tissues than in non-tumoral tissues (p <0.0001), with a positive correlation between mRNA and protein levels (r=0.56, p=0.01). Both mRNA and protein forms showed strong diagnostic performance (AUC=0.92 and AUC=0.82, respectively), consistent with observations across multiple tumor types. While CCNA2 protein levels showed prognostic relevance for OS (AUC=0.69, p=0.01), the mRNA-based prediction did not reach statistical significance (AUC=0.63, p=0.36). Functional enrichment analysis of CCNA2 co-expressed genes predicted involvement in cell cycle, mismatch repair, and DNA replication pathways. Additionally, protein-protein interaction analysis positioned CCNA2 as a central hub, suggesting its potential role in OTSCC pathogenesis.</p><p><strong>Conclusions: </strong>These findings indicate that CCNA2 is a promising diagnostic and prognostic biomarker candidate in OTSCC. Given the small size of the CPTAC validation cohort, further studies in larger, independent OTSCC cohorts are warranted to confirm its clinical utility.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"34 ","pages":"e20250618"},"PeriodicalIF":3.0,"publicationDate":"2026-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13123793/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147355021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of bleaching efficacy and cytotoxicity of an experimental hydrogel containing MnO-Doped Biosilicate® activated by violet LED light.","authors":"Rafael Dascanio, Rafael Antonio de Oliveira Ribeiro, Marina Trevelin Souza, Matheus Kury, Edgar Dutra Zanotto, Carlos Alberto de Souza Costa, Vanessa Cavalli","doi":"10.1590/1678-7765-2025-0503","DOIUrl":"10.1590/1678-7765-2025-0503","url":null,"abstract":"<p><p>Although hydrogen peroxide (HP) at 35% is effective in dental bleaching, the cytotoxicity associated with its use remains a significant concern. In this context, this study aimed to develop a bleaching hydrogel containing 6% HP, Biosilicate® (BioS) doped with manganese oxide (MnO_BioS), and irradiated with violet LED light (LED). Enamel/dentin discs were submitted to the following bleaching treatments (n=08): 35%HP (positive control), 6%HP gel containing BioS or MnO_BioS (0 and 10 wt%), with or without LED irradiation. The discs were adapted to artificial pulp chambers (APCs), with the enamel exposed for bleaching and the dentin facing the culture medium (DMEM). Bleaching was performed in three 30-minute sessions with 7-day intervals. After bleaching, the extracts (DMEM + bleaching gel components diffused through the discs) were collected and applied to the odontoblast-like MDPC-23 cells. Color change (ΔE00) and changes in the whiteness index (ΔWID) were determined before (T0) and after the last bleaching session (T2). Cell viability (MTT, %), HP diffusion (µg/mL), oxidative cell stress (OxS), and cell fluorescence (live/dead assay, by confocal microscopy) were assessed after the first session (T1). The data were analyzed using ANOVA and Tukey's test (p=0.05). The addition of 6%HP_MnO_BioS_LED exceeded ΔWID compared to 35%HP, showing statistical differences from the other groups, while ΔE00 was statistically similar to 35%HP. 6%HP_MnO_BioS_LED demonstrated higher cell viability, lower HP diffusion, and reduced oxidative stress (OxS) compared to the other groups (p<0.05). 6%HP_MnO_BioS_LED increased bleaching potential and presented lower cytotoxicity compared to 35%HP.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"34 ","pages":"e20250503"},"PeriodicalIF":3.0,"publicationDate":"2026-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13123781/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147354979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}