Journal of Applied Oral Science最新文献

{"title":"Bioactive luting cements on the adhesive interface of fiber post and root dentin: a comparative study of bond strength, water interaction, and enzymatic degradation.","authors":"Tiago Carvalho Dos Santos, Giovanna Speranza Zabeu, Ana Carolina Almeida Lima, Mylena Proença Costa, Marcela Rocha Carrilho, Rafael Francisco Lia Mondelli, Linda Wang","doi":"10.1590/1678-7765-2025-0576","DOIUrl":"10.1590/1678-7765-2025-0576","url":null,"abstract":"<p><strong>Objective: </strong>Innovative self-etching dental cements have been considered a versatile material with recent formulations incorporating bioactive additives. This study analyzed the role of these ingredients in systems used for luting intracanal posts.</p><p><strong>Methodology: </strong>Bond strength (BS), water sorption (WS), water solubility (WSB), and in situ zymography (IZ) were assessed. Three cements were tested: RelyX U200 (RU, bioactive-free control), RelyX Luting Plus (RL, fluoride-containing glass-ionomer), and BeautiCem SA (BC, S-PRG-based). Bond strength was evaluated in bovine root dentin (n=10) at the cervical, middle, and apical thirds at 7 days and 6 months. WS/WSB were assessed using 10 cement disks (10×1 mm) cycled through deionized water immersion (m1), desiccation (m2), and a second immersion (m3). For IZ, the specimens (n=3) were sectioned and incubated with fluorescein-conjugated gelatin for 48h at 37°C at both storage times (7 days and 6 months) for analysis by Confocal Laser Scanning Microscopy. Fluorescence intensity was quantified using Image J. Data were analyzed using ANOVA and Tukey's test (p<0.05).</p><p><strong>Results: </strong>RU presented the highest BS and the lowest WS/WSB, while RL showed the opposite trend. BC demonstrated intermediate performance with no statistical differences from the other materials. For all cements, the cervical third and the 6-month period yielded the highest BS values. WS/WSB were greater for the bioactive cements (BC, RL). IZ results aligned with BS findings, revealing suppressed enzymatic activity over time. Based on these results, it can be concluded that RU exhibited superior mechanical stability despite lacking bioactivity.</p><p><strong>Conclusion: </strong>Among bioactive cements, BC emerged as an interesting alternative, offering improved bond strength, water stability, and bioactive potential compared to RL.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"34 ","pages":"e20250576"},"PeriodicalIF":3.0,"publicationDate":"2026-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13123783/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147354924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Notch2 is involved in regulating apical tissue repair following severe intrusive luxation.","authors":"Yuanpei He, Xuyan Gong, Cancan Ding, Yang Qiu, Guangwen Li, Shiting Li","doi":"10.1590/1678-7765-2025-0490","DOIUrl":"10.1590/1678-7765-2025-0490","url":null,"abstract":"<p><strong>Methodology: </strong>This study aims to characterize apical repair dynamics in immature permanent teeth after severe intrusive luxation, and to explore Notch signaling pathway regulation in this process using a Sprague-Dawley rat model. In this study, a modified tool was utilized to establish an intrusive luxation model in SD rats. Tissue repair was assessed via micro-computed tomography (Micro-CT) and hematoxylin-eosin (H&E) staining. Differential gene expression analysis of stem cells from the apical papilla (SCAPs) was performed using the publicly available GEO dataset. Immunohistochemical detection of Notch2 receptor expression in the apical region of injured teeth was subsequently conducted, guided by bioinformatics screening. Finally, the Notch signaling pathway was pharmacologically inhibited using DAPT (γ-secretase inhibitor), and its impact on post-injury prognosis was evaluated.</p><p><strong>Results: </strong>Histological analysis revealed prevalent pulp hypoplasia and apical tissue fibrosis after injury. Differential gene analysis suggested that the Notch signaling pathway, particularly Notch2, is involved in the regulation of angiogenesis-related pathways in SCAPs. Immunohistochemistry showed positive Notch2 expression in fibrotic apical regions. Notably, inhibition of Notch signaling significantly reduced aberrant fibrosis while enhancing vascular proliferation in the injury site.</p><p><strong>Conclusions: </strong>Severe intrusive luxation is primarily characterized by aberrant fibrous differentiation in the apical region. The Notch signaling pathway may negatively regulate angiogenesis in the damaged apical area, suggesting that targeted inhibition of this pathway could promote tissue repair following embedded dental injuries.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"34 ","pages":"e20250490"},"PeriodicalIF":3.0,"publicationDate":"2026-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13123771/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147355164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Maqui berry cystatin inhibits cathepsin k activity and stimulates osteogenic differentiation of human dental pulp cells.","authors":"Luana Raphael da Silva, Eduardo Pereira de Souza, Bárbara Roma Mendes, Breno Henrique Amancio, Flávio Henrique-Silva, Gisele Faria","doi":"10.1590/1678-7765-2025-0661","DOIUrl":"10.1590/1678-7765-2025-0661","url":null,"abstract":"<p><strong>Objectives: </strong>Cystatins, endogenous inhibitors of cysteine proteases, regulate extracellular matrix degradation. Their plant-derived homologs (phytocystatins) include MaquiCPI-3, a recombinant protein obtained from Aristotelia chilensis (maqui berry). This study aimed to investigate the inhibitory effect of MaquiCPI-3 on human cathepsin K (CTSK) activity and its cytotoxicity and impact on the proliferation, migration, and osteogenic differentiation of human dental pulp cells (hDPCs).</p><p><strong>Methodology: </strong>The inhibitory activity of MaquiCPI-3 against CTSK was measured using a spectrofluorometer with the fluorogenic substrate Z-Phe-Arg-AMC. The hDPCs from third molars were characterized by flow cytometry for mesenchymal (CD90, CD73, CD105) and hematopoietic (CD34, CD45) markers. The hDPCs, either exposed to MaquiCPI-3 or left untreated (control), were assessed for viability (MTT assay), proliferation (bromodeoxyuridine incorporation), chemotaxis (Transwell assay), mineralized nodule formation (Alizarin Red S staining), alkaline phosphatase activity (thymolphthalein release), and expression of mineralization-related genes (qPCR). Data were analyzed using one- or two-way ANOVA with appropriate post hoc tests or nonparametric alternatives (α=0.05).</p><p><strong>Results: </strong>MaquiCPI-3 potently inhibited CTSK (Ki=1.72 nM, Ki,app=2.08 nM), showed no cytotoxicity, and significantly enhanced ALP activity, mineralized nodule formation, and expression of BMP-2 and osteocalcin, stimulating no hDPC proliferation or migration when compared with the control.</p><p><strong>Conclusions: </strong>MaquiCPI-3 increased no cell proliferation or migration, its ability to inhibit CTSK activity and induce an osteogenic phenotype shows promising potential therapeutic strategies aimed at repairing and regenerating pulp and periapical tissues and controlling bone resorption.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"34 ","pages":"e20250661"},"PeriodicalIF":3.0,"publicationDate":"2026-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13123779/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146226807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of bromelain, melatonin, and thymoquinone on neutrophil extracellular trap formation and macrophage polarization in experimental periodontitis.","authors":"Şükran Acipinar, Mustafa Ozkaraca, Ufuk Okkay, Ali Sefa Mendil","doi":"10.1590/1678-7765-2025-0699","DOIUrl":"10.1590/1678-7765-2025-0699","url":null,"abstract":"<p><strong>Objective: </strong>Periodontitis is a chronic inflammatory disease characterized by dysregulated immune responses leading to alveolar bone loss. Formation of neutrophil extracellular traps and macrophage polarization play critical roles in its pathogenesis, and natural agents with immunomodulatory properties are being investigated as potential adjunctive therapies. This study aimed to compare the effects of systemically administered melatonin, bromelain, and thymoquinone on serum neutrophil extracellular trap formation and macrophage polarization in experimental periodontitis.</p><p><strong>Methodology: </strong>Forty rats were assigned to five groups: control, experimental periodontitis, and experimental periodontitis treated with melatonin, bromelain, or thymoquinone. Periodontitis was induced by ligature placement for 14 days. After ligature removal, the treatment groups received daily oral doses of melatonin, bromelain, or thymoquinone for 14 consecutive days. Biochemical, histologic, histomorphometric, and immunohistochemical analyses were performed. Comparisons between groups were performed using one-way ANOVA. Neutrophil extracellular trap levels measured before and after treatment within the same group were analyzed using a paired t-test.</p><p><strong>Results: </strong>Thymoquinone treatment significantly reduced alveolar bone loss compared to the bromelain and melatonin groups (p=0.00). The highest value was observed in the Periodontitis group and the lowest in the Thymoquinone group for neutrophil extracellular trap levels, with intergroup comparisons showing no statistically significant differences (p=0.597). All immunohistochemical analyses showed significant differences in all treatment groups compared to both the control and periodontitis groups (p=0.000). CD68(+) macrophages were most abundant in the periodontitis group and least in the Melatonin group (p=0.00). CD80 expression was highest in the Melatonin group and lowest in the Thymoquinone group (p=0.000), while CD206 expression was highest in the Bromelain group and lowest in the Thymoquinone group (p=0.00).</p><p><strong>Conclusion: </strong>Thymoquinone exhibited comparatively more favorable patterns, and overall, all agents showed trends that may support their consideration as adjunctive approaches in periodontitis management.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"34 ","pages":"e20250699"},"PeriodicalIF":3.0,"publicationDate":"2026-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13123786/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146226701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Factors associated with orofacial myofunctional condition in patients with temporomandibular disorder and somatosensory tinnitus.","authors":"Gislaine Aparecida Folha, Nelma Ellen Zamberlan-Amorim, Carina Ferreira Pinheiro-Araujo, Luana Maria Ramos Mendes, Luana Denadai Oliveira Menezes, Gesseany Joice Rodrigues Dos Santos, Debora Bevilaqua-Grossi","doi":"10.1590/1678-7765-2025-0515","DOIUrl":"10.1590/1678-7765-2025-0515","url":null,"abstract":"<p><strong>Objective: </strong>Tinnitus is frequently reported in individuals with temporomandibular disorders (TMD), especially in the somatosensory subtype, which can be modulated by afferent input from orofacial and cervical structures. While the impact of orofacial dysfunctions on tinnitus has been explored, the potential reverse relationship remains understudied. To investigate the association between somatosensory tinnitus and orofacial myofunctional condition in individuals with TMD.</p><p><strong>Methodology: </strong>An observational, cross-sectional study was conducted with 47 adults aged 18 to 45 years diagnosed with TMD and self-reported tinnitus. Participants were assessed in a university-based outpatient setting. The primary outcome was the orofacial myofunctional condition, measured using the Orofacial Myofunctional Evaluation with Scores (OMES) protocol. Additional variables included tinnitus intensity and frequency (via acuphenometry), tinnitus-related distress (Tinnitus Handicap Inventory - THI), cervical disability (Neck Disability Index - NDI), craniofacial pain and disability (Craniofacial Pain and Disability Inventory - CF-PDI), and psychological symptoms (Hospital Anxiety and Depression Scale - HADS). Data were analyzed using Spearman correlation and multiple linear regression, and adopted statistical significance was set at p<0.05.</p><p><strong>Results: </strong>OMES scores showed negative correlations with tinnitus intensity (r=-0.353), THI, NDI, CF-PDI, and age. In the final regression model, only tinnitus intensity remained a predictor of OMES score (β =-0.356; R2=0.127, Confidence Interval -0.20 to -0.02), indicating that greater tinnitus intensity was associated with poorer orofacial myofunctional condition.</p><p><strong>Conclusion: </strong>These findings suggest that somatosensory tinnitus may negatively impact orofacial motor function in patients with TMD, underscoring the need for integrated and multidisciplinary assessment approaches.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"34 ","pages":"e20250515"},"PeriodicalIF":3.0,"publicationDate":"2026-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13123766/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146226681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DNA methyltransferases expressions in mice tongue exposed to waterpipe smoke.","authors":"Carolina Simão Flausino, Sarah Freygang Mendes Pilati, Filipe Ivan Daniel, Filipe Modolo","doi":"10.1590/1678-7765-2025-0665","DOIUrl":"10.1590/1678-7765-2025-0665","url":null,"abstract":"<p><strong>Objective: </strong>Waterpipe smoking has gained widespread use nowadays, especially among young adults. As this type of smoke has been described to contain toxins and carcinogens, this study evaluated DNA methyltransferase (DNMT) 1 and 3b expression, and inflammation, on the dorsal surface, ventral surface and lateral border of Swiss mice's tongues exposed to waterpipe smoke.</p><p><strong>Methodology: </strong>Animals were divided into 6 groups (n=60): control, 7, 15, 30, 60, and 90 days of consecutive exposure to waterpipe smoke in a whole-body exposure system. After each period, tongues were analyzed using hematoxylin/eosin staining for inflammation status and immunohistochemistry for DNMT1 and DNMT3b.</p><p><strong>Results: </strong>DNMT3b showed lower immunoexpression from 7 to 60 days; at 90 days, expression was similar to that of control or there was upregulation on the ventral surface compared with control. DNMT1 exhibited lower expression at all exposure times, with the ventral surface showing similar expression to that of control at 90 days. Waterpipe smoke was not able to induce acute or chronic inflammation in mice tongue.</p><p><strong>Conclusion: </strong>Waterpipe smoke may result in a DNA hypomethylation pattern in initial exposure periods, contributing to activate proto-oncogenes and/or genomic instability. Over long periods, it may lead to a methylation pattern similar to that of control or even to hypermethylation, silencing tumor suppressor genes. These alterations in the genome due to hypo/hypermethylation contribute largely for the development of diseases such as oral cancer.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"34 ","pages":"e20250665"},"PeriodicalIF":3.0,"publicationDate":"2026-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13123776/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146226692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shades of knowledge: awareness among Brazilian dentists on non- and microinvasive approaches for tooth discoloration.","authors":"Giovanni Aguirra Liberatti, Karin Landmayer, Bruna de Oliveira Iatarola, Maria Paula Novaes Camargo Manna, Raquel Shimizu Mori, Flávia Pardo Salata Nahsan, Heitor Marques Honório, Luciana Fávaro Francisconi-Dos-Rios","doi":"10.1590/1678-7765-2025-0589","DOIUrl":"10.1590/1678-7765-2025-0589","url":null,"abstract":"<p><strong>Objective: </strong>Tooth discoloration is a frequent concern among dental patients. While many professionals and patients choose veneers or other invasive solutions, Minimal Intervention Dentistry (MID) recommends non- and microinvasive approaches to preserve tooth structure. To assess Brazilian dentists' knowledge and practices on tooth discoloration and how education and professional profile may influence them.</p><p><strong>Methodology: </strong>A cross-sectional observational study was conducted with 384 volunteer dentists via an online series of questions on causes, diagnosis, treatment (per tissue removal), and professional-patient relationship. Answers were scored (1=correct/affirmative, 0=incorrect/negative). Descriptive and multivariate regression analyses were performed (α=0.05).</p><p><strong>Results: </strong>Most respondents were female, aged 20-49, from the Southeast region. Correct answer rates: 69.9% for causes, 69.4% for diagnosis, 48.8% for treatment, 83.6% for professional-patient relationship, 56.4% overall. Graduation from public universities and holding graduate training in Operative or Pediatric Dentistry or Orofacial Harmonization were associated with better outcomes.</p><p><strong>Conclusion: </strong>Knowledge of non- and microinvasive approaches to tooth discoloration remains limited. Broader educational efforts are needed, especially among specific groups of professionals who showed lower performance.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"34 ","pages":"e20250589"},"PeriodicalIF":3.0,"publicationDate":"2026-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13123772/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146226755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diversity of Streptococcus mutans serotypes isolated from dental plaque of Brazilian patients with a history of acute myocardial infarction.","authors":"Francisco Ruliglésio Rocha, Wanessa Fernandes Matias Regis, Rochelle Pinheiro Ribeiro, João Eudes Teixeira Pinho Filho, Tiago Lima Sampaio, Raquel Carvalho Montenegro, Ana Paula Negreiros Nunes Alves, Lidiany Karla Azevedo Rodrigues","doi":"10.1590/1678-7765-2025-0077","DOIUrl":"10.1590/1678-7765-2025-0077","url":null,"abstract":"<p><strong>Background: </strong>Streptococcus mutans serotypes (c, e, f, k) are linked to dental caries, with less common serotypes (e, f, k) and collagen-binding genes (CBG: cnm and cbm) suggested to be associated with cardiovascular diseases.</p><p><strong>Objective: </strong>This study investigated the presence of S. mutans serotypes and collagen-binding genes (CBGs) in dental plaque and their possible association with acute myocardial infarction (AMI) in adults.</p><p><strong>Methodology: </strong>A total of 31 infarcted and 17 non-infarcted patients underwent oral and blood examinations. DNA from plaque samples was analyzed using PCR to identify S. mutans serotypes and cnm/cbm genes. S. mutans was detected in 22.6% (7/31) of infarcted patients and 11.8% (2/17) of non-infarcted patients.</p><p><strong>Results: </strong>Serotype c was the most prevalent in infarcted patients (57.1%, 4/7), followed by e (42.9%, 3/7) and k (14.3%, 1/7); serotype f was not detected. Only serotype c was found in non-infarcted patients. Less common serotypes (e, k) and co-occurring serotypes (c, k) were exclusive to infarcted patients. No CBGs were detected in any serotypes. No association was found between S. mutans and dental caries in either group. Patients with cholesterol levels <190 mg/dL and no S. mutans had a 47.4% lower risk of infarction.</p><p><strong>Conclusion: </strong>Although S. mutans, including less common serotypes, was more prevalent in infarcted patients, no significant association with AMI was observed. Further research is needed to elucidate the role of S. mutans in cardiovascular diseases.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"34 ","pages":"e20250077"},"PeriodicalIF":3.0,"publicationDate":"2026-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13123788/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146118882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SHED-exosomes functionalized micro-nano bioactive glass/PCL membrane regulates macrophage polarization and promotes osteogenic differentiation.","authors":"Yongyong Yan, Haifeng Lan, Guohou Miao, Haiyan Wang, Zhengmao Li, Qing Zhang, Shuai Wang, Gang Wu, Richard T Jaspers, Janak L Pathak","doi":"10.1590/1678-7765-2025-0541","DOIUrl":"10.1590/1678-7765-2025-0541","url":null,"abstract":"<p><p>Micro-nano bioactive glass (MNBG) has bone regenerative potential. However, the difficulty of MNBG molding restricts its clinical applications in bone regeneration. Exosomes carry proteins, lipids, and nucleic acids for communication between cells. In this study, we adhered human exfoliated deciduous teeth (SHED)-derived exosomes (SHED-Exos) to electrospun micro-nano bioactive glass/polycaprolactone (MNBG/PCL) membrane to control inflammation and promote bone regeneration. MNBG/PCL membrane showed biocompatibility and osteogenic ability. Next, we demonstrated that SHED-Exos internalized into macrophages attenuated LPS-induced expression of M1-macrophage markers iNOS, IL-6, and IL-1β and upregulated M2-macrophage marker IL-10 expression, indicating a switch from M1 to M2 macrophage phenotype. MNBG/PCL-loaded SHED-Exos membrane supported the survival and growth of mouse bone marrow stromal cells (mBMSCs). When M1 macrophages were co-cultured with mBMSCs on the membrane, the SHED-Exos-loaded membrane showed higher Runx2, Alp, and Ocn gene expression. Our findings indicate that SHED-Exos-functionalized MNBG/PCL membrane has anti-inflammatory and osteoinductive potential, suggesting its potential as a candidate material for bone regeneration.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"34 ","pages":"e20250541"},"PeriodicalIF":3.0,"publicationDate":"2026-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13123774/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146118983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic variants of EBI3, tumor Epstein-Barr virus, and human cytomegalovirus status in HPV-negative oral cancer.","authors":"Marko Simonovic, Ruzica Kozomara, Sasa Jovic, Gordana Velikic, Elizabeta Ristanovic, Nikoleta Djordjevski, Danijela Djuric-Petkovic, Srboljub Stosic, Gordana Supic","doi":"10.1590/1678-7765-2025-0492","DOIUrl":"10.1590/1678-7765-2025-0492","url":null,"abstract":"<p><strong>Objective: </strong>This study evaluated the clinical relevance of EBI3 polymorphisms, along with tumor Epstein-Barr virus (EBV) and Human Cytomegalovirus (HCMV) status, in prognostically adverse HPV-negative OSCCs.</p><p><strong>Methodology: </strong>EBI3 (rs4740, rs4905, rs428253) genotyping was performed by qPCR in 95 HPV-negative OSCC patients and 108 age- and sex-matched controls. Tumor HPV, EBV, and HCMV status were assessed by qPCR. EBV viral load was calculated by exponential approach and a relative estimate of EBV copies per 105 cells. Associations with overall survival (OS) and recurrence-free survival (RFS) were evaluated by Kaplan-Meier and Cox regression analyses.</p><p><strong>Results: </strong>EBV-positive tumors showed a significant association with increasing nodal stage (P=0.020). EBV viral load stratification (negative, low, high) presented a non-significant trend toward association with advanced tumor stage (P=0.060). Notably, EBI3 rs428253 predicted worse OS in EBV-positive patients, whereas rs4740 and rs4905 variants were associated with advanced tumor stage (P=0.024 and P=0.018). rs4740 and rs4905 variants were inversely associated with OSCC risk in dominant and overdominant models. Analysis detected HCMV in 7.4% of tumors but was not clinically relevant.</p><p><strong>Conclusions: </strong>EBI3 genetic variants and EBV status may have prognostic relevance in HPV-negative OSCC. EBV may interact with the host genetics to influence nodal metastases and outcomes, suggesting a potential EBV-EBI3 axis, which warrants further investigation. Future precision oncology approaches may incorporate host and viral genetic markers to identify and stratify high-risk HPV-negative OSCC patients.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"34 ","pages":"e20250492"},"PeriodicalIF":3.0,"publicationDate":"2026-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13123764/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146085872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}