{"title":"Histological evaluation of the surgical margins of oral soft tissue incisions using a dual-wavelength diode laser and an Er, Cr:YSGG laser; an ex vivo study.","authors":"Alaa Jamal Al-Ani, Hanan J Taher, Ammar Saleh Alalawi","doi":"10.1590/1678-7757-2023-0419","DOIUrl":"https://doi.org/10.1590/1678-7757-2023-0419","url":null,"abstract":"<p><strong>Objective: </strong>This study compared a dual-wavelength diode laser and an Er, Cr:YSGG laser in oral soft tissue incisions to determine the most effective and safest laser system at the histopathological level.</p><p><strong>Methodology: </strong>The (810 and 980 nm) dual-wavelength diode laser was used at 1.5 W and 2.5 W (CW) power settings, and the (2780 nm) Er, Cr:YSGG laser was used at 2.5 W and 3.5 W (PW) power settings. Both laser systems were used to incise the tissues of freshly dissected sheep tongue pieces to obtain the following histopathological criteria: epithelial tissue changes, connective tissue changes, and lateral thermal damage extent by optical microscopy.</p><p><strong>Results: </strong>The epithelial and connective tissue damage scores were significantly higher in the dual-wavelength diode laser groups than in the Er, Cr:YSGG laser groups (P<0.001), and there was a significant difference between some groups. The extent of lateral thermal damage was also significantly higher in the diode laser groups than in the Er, Cr: YSGG laser groups (P<0.001), and there was a significant difference between groups. Group 2 (2.5 W) of the diode laser was the highest for all three criteria, while group 3 (2.5 W) of the Er, Cr:YSGG laser was the lowest.</p><p><strong>Conclusion: </strong>The Er, Cr:YSGG laser with an output power of 2.5 W is, histologically, the most effective and safest laser for oral soft tissue incision. The dual-wavelength diode laser causes more damage than the Er, Cr:YSGG laser, but it can be used with a low output power and 1 mm safety distance in excisional biopsy.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"32 ","pages":"e20230419"},"PeriodicalIF":2.7,"publicationDate":"2024-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140858650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Beatriz de Camargo Poker, Viviane de Cássia Oliveira, Ana Paula Macedo, Mariane Gonçalves, Ana Paula Ramos, Cláudia Helena Silva-Lovato
{"title":"Evaluation of surface roughness, wettability and adhesion of multispecies biofilm on 3D-printed resins for the base and teeth of complete dentures.","authors":"Beatriz de Camargo Poker, Viviane de Cássia Oliveira, Ana Paula Macedo, Mariane Gonçalves, Ana Paula Ramos, Cláudia Helena Silva-Lovato","doi":"10.1590/1678-7757-2023-0326","DOIUrl":"https://doi.org/10.1590/1678-7757-2023-0326","url":null,"abstract":"<p><strong>Objective: </strong>This study evaluated the surface roughness, wettability and adhesion of multispecies biofilms (Candida albicans, Staphylococcus aureus and Streptococcus mutans) on 3D-printed resins for complete denture bases and teeth compared to conventional resins (heat-polymerized acrylic resin; artificial pre-fabricated teeth).</p><p><strong>Methodology: </strong>Circular specimens (n=39; 6.0 mm Ø × 2.0 mm) of each group were subjected to roughness (n=30), wettability (n=30) and biofilm adhesion (n=9) tests. Three roughness measurements were taken by laser confocal microscopy and a mean value was calculated. Wettability was evaluated by the contact angle of sessile drop method, considering the mean of the three evaluations per specimen. In parallel, microorganism adhesion to resin surfaces was evaluated using a multispecies biofilm model. Microbial load was evaluated by determining the number of Colony Forming Units (CFU/mL) and by scanning electron microscopy (SEM). Data were subjected to the Wald test in a generalized linear model with multiple comparisons and Bonferroni adjustment, as well as two-way ANOVA (α=5%).</p><p><strong>Results: </strong>The roughness of the conventional base resin (0.01±0.04) was lower than that of the conventional tooth (0.14±0.04) (p=0.023) and 3D-printed base (0.18±0.08) (p<0.001). For wettability, conventional resin (84.20±5.57) showed a higher contact angle than the 3D-printed resin (60.58±6.18) (p<0.001). Higher microbial loads of S. mutans (p=0.023) and S. aureus (p=0.010) were observed on the surface of the conventional resin (S. mutans: 5.48±1.55; S. aureus: 7.01±0.57) compared to the 3D-printed resin (S. mutans: 4.11±1.96; S. aureus: 6.42±0.78). The adhesion of C. albicans was not affected by surface characteristics. The conventional base resin showed less roughness than the conventional dental resin and the printed base resin.</p><p><strong>Conclusion: </strong>The 3D-printed resins for base and tooth showed less hydrophobicity and less adhesion of S. mutans and S. aureus than conventional resins.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"32 ","pages":"e20230326"},"PeriodicalIF":2.7,"publicationDate":"2024-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140855127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Ultrasonographic analysis of palatal donor site healing accelerated with platelet-rich fibrin following subepithelial connective tissue harvesting.","authors":"Gizem Torumtay Cin, Aysan Lektemur Alpan, Gözde Açikgöz, Gülfem Özlü Uçan","doi":"10.1590/1678-7757-2023-0448","DOIUrl":"https://doi.org/10.1590/1678-7757-2023-0448","url":null,"abstract":"<p><strong>Objective: </strong>Platelet-rich fibrin (PRF) contains a variety of growth factors and bioactive molecules that play crucial roles in wound healing and angiogenesis. We aimed to evaluate the effects of PRF on tissue thickness and vascularization of the palatal donor site by ultrasound (USG) following subepithelial connective tissue harvesting.</p><p><strong>Methodology: </strong>A subepithelial connective tissue graft was harvested from the palatal region with a single incision for root coverage in 20 systemically healthy patients. In the test group (n = 10), the PRF membrane was placed at the donor site, whereas no material was applied in the control group (n=10). Palatal tissue thickness (PTT) and pulsatility index (PI) were evaluated by USG at baseline and on the 3rd, 7th, 14th, 30th, and 90th days after surgery. The early healing index (EHI) was used to evaluate donor site healing for 30 days.</p><p><strong>Results: </strong>PTT was significantly higher in the PRF group on the 3rd and 14th days after surgery when compared to the controls. In the PRF-treated group, PI levels were significantly higher than in the controls, especially on the 14th day. PTT increased significantly 90 days after surgery compared to the test group baseline, but controls showed a significant decrease. The PRF group showed statistically significant improvements in EHI scores compared to controls on days 3, 7, and 14. This study found a negative correlation between PI values and EHI scores on postoperative days three and seven in the test group.</p><p><strong>Conclusion: </strong>USG is a non-invasive, objective method to radiographically evaluate the regenerative effects of PRF on palatal wound healing after soft tissue harvesting. To overcome graft inadequacy in reharvesting procedures, PRF application may enhance clinical success and reduce possible complications by increasing tissue thickness and revascularization in the donor area.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"32 ","pages":"e20230448"},"PeriodicalIF":2.7,"publicationDate":"2024-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140851881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Suzan Seif Allah Ibrahim, Ibrahim Abu Mandil, Ola Mohamed Ezzatt
{"title":"Injectable platelet rich fibrin effect on laser depigmented gingiva: a clinical randomized controlled split mouth trial with histological assessment.","authors":"Suzan Seif Allah Ibrahim, Ibrahim Abu Mandil, Ola Mohamed Ezzatt","doi":"10.1590/1678-7757-2022-0307","DOIUrl":"10.1590/1678-7757-2022-0307","url":null,"abstract":"<p><strong>Objective: </strong>To determine whether intra-mucosal injection of injectable platelet-rich fibrin (i-PRF) can promote healing after Diode Laser Gingival Depigmentation (DLGD).</p><p><strong>Methodology: </strong>A total of 20 arch sites of hyperpigmented gingiva of 10 patients underwent DLGD. For each patient, two arch sites were randomly assigned for either intra-mucosal injection of i-PRF (G1-i-PRF) (n=10 sites) or no treatment (G2-Control): (n=10 sites). Wound Healing Score (WHS), patient satisfaction, and Pigmentation Index (DOPI) were measured at 1 week and 1 and 3 months postoperatively. Histological assessment of tissue specimens was performed at baseline and 1 week.</p><p><strong>Results: </strong>The percentage change in WHS at 1 week was significantly higher in G1 (58.34±15.43) compared to G2 (37.50±11.79). At day 1, 50% of patients in G1 were pain free compared with 75% in G2, who had mild pain. Mean DOPI decreased significantly at 3 months in both groups (P-value <0.001), without significant differences between groups. G1 specimens showed significantly higher epithelial thickness (P-value <0.001), as well as a higher number of blood vessels and less percentage of inflammatory cells.</p><p><strong>Conclusions: </strong>i-PRF demonstrated better clinical and histological healing potential and less patient discomfort compared to sites without treatment after DLGD. Registered at https://clinicaltrials.gov/ as (NCT05283668).</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"32 ","pages":"e20230307"},"PeriodicalIF":2.7,"publicationDate":"2024-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11018297/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140305720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
William Phillip Pereira da Silva, João Matheus Fonseca E Santos, Mônica Caroline de Souza, Stéfany Barbosa, Anderson Maikon de Souza Santos, Edilson Ervolino, Ana Paula Farnezi Bassi, Cortino Sukotjo, Leonardo P Faverani
{"title":"Ozone therapy improves early stages of osseointegration in ovariectomized rats.","authors":"William Phillip Pereira da Silva, João Matheus Fonseca E Santos, Mônica Caroline de Souza, Stéfany Barbosa, Anderson Maikon de Souza Santos, Edilson Ervolino, Ana Paula Farnezi Bassi, Cortino Sukotjo, Leonardo P Faverani","doi":"10.1590/1678-7757-2023-0172","DOIUrl":"10.1590/1678-7757-2023-0172","url":null,"abstract":"<p><strong>Objective: </strong>the aim of this study was to analyze the influence of ozone therapy (OZN) on peri-implant bone repair in critical bones by installing osseointegrated implants in the tibia of ovariectomized rats.</p><p><strong>Methodology: </strong>ovariectomy was performed on 30 Wistar rats, aged six months (Rattus novergicus), and, after 90 days, osseointegrated implants were installed in each tibial metaphysis. The study groups were divided into the animals that received intraperitoneal ozone at a concentration of 700 mcg/kg - OZ Group (n=15) - and a control group that received an intraperitoneal saline solution and, for this reason, was named the SAL group (n=15). The applications for both groups occurred during the immediate post-operative period on the 2nd, 4th, 6th, 8th, 10th, and 12th day post-surgery. At various stages (14, 42, and 60 days), the animals were euthanized, and tests were performed on their tibiae. These tests include histomorphometric and immunohistochemical analyses, computerized microtomography, sampling in light-cured resin for calcified sections, and confocal microscopy. The obtained data were then analyzed using One-way ANOVA and the Shapiro-Wilk, Kruskal-Wallis, and student t-tests (P<0.05).</p><p><strong>Results: </strong>our findings indicate that the OZ group (3.26±0.20 mm) showed better cellular organization and bone neoformation at 14 days (SAL group, 0.90±1.42 mm) (P=0.001). Immunohistochemistry revealed that osteocalcin labeling was moderate in the OZ group and mild in the SAL group at 14 and 42 days post-surgery. The data from the analysis of calcified tissues (microtomography, histometric, and bone dynamism analysis) at 60 days showed no statistically significant differences between the groups (P=0.32).</p><p><strong>Conclusion: </strong>it was concluded that ozone therapy anticipated the initial phases of the peri-implant bone repair process.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"32 ","pages":"e20230172"},"PeriodicalIF":2.7,"publicationDate":"2024-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11018298/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140305742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rüdiger Emshoff, Magdalena Astl, Aris Ioannis Giotakis, Linus Christian Hupp, Andreas Kolk
{"title":"Factors associated with voice-related quality of life among patients with temporomandibular disorders.","authors":"Rüdiger Emshoff, Magdalena Astl, Aris Ioannis Giotakis, Linus Christian Hupp, Andreas Kolk","doi":"10.1590/1678-7757-2023-0296","DOIUrl":"10.1590/1678-7757-2023-0296","url":null,"abstract":"<p><strong>Background: </strong>Research would be important for obtaining a better understanding of voice complaints among patients with temporomandibular disorders (TMD).</p><p><strong>Objective: </strong>The identification of predictors of voice disorders associated with TMD pain was made according to Diagnostic Criteria for TMD (DC/TMD) Axis I.</p><p><strong>Methodology: </strong>Functional limitations were measured using the Jaw Functional Limitation Scales for mastication (JFLS-M), jaw mobility (JFLS-JM), and verbal and emotional expression (JFLS-VEE). Patients also completed the Hospital Anxiety and Depression Scale (HADS). The primary outcome was social-emotional and physical functioning as indicated by scores on the Voice-Related Quality of Life (V-RQOL) questionnaire. Multiple linear regression was used to model the relationship between the domains on the V-RQOL questionnaire and scores on the HADS and JFLS after adjusting for age, gender, DC/TMD diagnosis, pain intensity, and time since pain onset.</p><p><strong>Results: </strong>The HADS-D (B=-1.15; 95% CI, -1.718 to -0.587; p<.001) and JFLS-VEE (B=-0.22; 95% CI, -0.40 to -0.06; p=.008) were significant predictors of scores on the V-RQOL questionnaire.</p><p><strong>Conclusion: </strong>Higher scores on depression measures and limitations in verbal and emotional expression could exacerbate voice problems among TMD pain patients. Future research should promote multidisciplinary treatments for TMD pain-related voice disorders.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"32 ","pages":"e20230296"},"PeriodicalIF":2.7,"publicationDate":"2024-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10984579/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140305718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Taynara de Souza Carneiro, Michael Willian Favoreto, João Pedro Ferreira Rodrigues, Elisama Sutil, Gabrielle Gomes Centenaro, Isabela de Matos de Freitas, Alessandra Reis, Laura Ceballos García, Alessandro Dourado Loguercio
{"title":"In-office dental bleaching in adolescents using 6% hydrogen peroxide with and without gingival barrier: a randomized double-blind clinical trial.","authors":"Taynara de Souza Carneiro, Michael Willian Favoreto, João Pedro Ferreira Rodrigues, Elisama Sutil, Gabrielle Gomes Centenaro, Isabela de Matos de Freitas, Alessandra Reis, Laura Ceballos García, Alessandro Dourado Loguercio","doi":"10.1590/1678-7757-2023-0416","DOIUrl":"10.1590/1678-7757-2023-0416","url":null,"abstract":"<p><strong>Background: </strong>At low concentrations used for in-office bleaching gels, such as 6% HP, gingival barrier continues to be performed. If we take into account that, in the at-home bleaching technique, no barrier is indicated, it seems that the use of a gingival barrier fails to make much sense when bleaching gel in low concentration is used for in-office bleaching.</p><p><strong>Objective: </strong>This double-blind, split-mouth, randomized clinical trial evaluated the gingival irritation (GI) of in-office bleaching using 6% hydrogen peroxide (HP) with and without a gingival barrier in adolescents, as well as color change and the impact of oral condition on quality of life.</p><p><strong>Methodology: </strong>Overall, 60 participants were randomized into which side would or would not receive the gingival barrier. In-office bleaching was performed for 50 minutes with 6% HP in three sessions. The absolute risk and intensity of GI were assessed with a visual analogue scale. Color change was assessed using a digital spectrophotometer and color guides. The impact of oral condition on quality of life was assessed using the Brazilian version of the Oral Health Impact Profile (α=0.05).</p><p><strong>Results: </strong>The proportion of patients who presented GI for the \"with barrier\" group was 31.6% and for the \"without barrier\" group, 30% (p=1.0). There is an equivalence for the evaluated groups regarding GI intensity (p<0.01). Color change was detected with no statistical differences (p>0.29). There was a significant impact of oral condition on quality of life after bleaching (p<0.001).</p><p><strong>Conclusions: </strong>The use or not of the gingival barrier for in-office bleaching with 6% HP was equivalent for GI, as well as for bleaching efficacy, with improvement in the impact of oral condition on quality of life.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"32 ","pages":"e20230416"},"PeriodicalIF":2.7,"publicationDate":"2024-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11037928/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140305721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Anna Clara Gurgel Gomes, Janaina Gomes Maciel, Amanda Aparecida Maia Neves Garcia, Letycia Accioly Simões Coelho, Giulia Murcia Rodrigues, Vinicius Carvalho Porto, Grigorios Polyzois, Marlise Inêz Klein, Simone Soares, Vanessa Migliorini Urban, Karin Hermana Neppelenbroek
{"title":"Anti-biofilm effectiveness of protocols for cleaning complete dentures in hospitalized patients: a randomized controlled trial.","authors":"Anna Clara Gurgel Gomes, Janaina Gomes Maciel, Amanda Aparecida Maia Neves Garcia, Letycia Accioly Simões Coelho, Giulia Murcia Rodrigues, Vinicius Carvalho Porto, Grigorios Polyzois, Marlise Inêz Klein, Simone Soares, Vanessa Migliorini Urban, Karin Hermana Neppelenbroek","doi":"10.1590/1678-7757-2022-0381","DOIUrl":"10.1590/1678-7757-2022-0381","url":null,"abstract":"<p><strong>Background: </strong>Denture biofilm acts as a potential reservoir for respiratory pathogens, considerably increasing the risk of lung infections, specifically aspiration pneumonia, mainly 48h after hospital admission. The establishment of a straightforward, affordable, and applicable hygiene protocol in a hospital environment for the effective control of denture biofilm can be particularly useful to prevent respiratory infections or reduce the course of established lung disease.</p><p><strong>Objectives: </strong>To evaluate the anti-biofilm effectiveness of denture cleaning protocols in hospitalized patients.</p><p><strong>Methodology: </strong>The maxillary complete dentures (MCDs) of 340 hospitalized participants were randomly cleaned once using one of the following 17 protocols (n=20): brushing with distilled water, toothpaste, or neutral liquid soap (controls); immersion in chemical solutions (1% sodium hypochlorite, alkaline peroxide, 0.12% or 2% chlorhexidine digluconate), or microwave irradiation (650 W for 3 min) combined or not with brushing. Before and after the application of the protocols, the biofilm of the intaglio surface of the MCDs was evaluated using two methods: denture biofilm coverage area (%) and microbiological quantitative cultures on blood agar and Sabouraud Dextrose Agar (CFU/mL). Data were subjected to the Wilcoxon and Kruskal-Wallis tests (α=0.05).</p><p><strong>Results: </strong>All 17 protocols significantly reduced the percentage area of denture biofilm and microbial and fungal load (P<0.05). The highest percentage reductions in the area of denture biofilm were observed for 1% hypochlorite solution with or without brushing and for 2% chlorhexidine solution and microwave irradiation only in association with brushing (P<0.05). The greatest reductions in microbial and fungal load were found for the groups that used solutions of 2% chlorhexidine and 1% hypochlorite and microwave irradiation, regardless of the association with brushing (P<0.05).</p><p><strong>Conclusions: </strong>A single immersion for 10 min in 1% sodium hypochlorite, even in the absence of brushing, proved to be a straightforward, rapid, low-cost, and effective protocol for cleaning the dentures of hospitalized patients.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"32 ","pages":"e20230381"},"PeriodicalIF":2.7,"publicationDate":"2024-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10984580/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140305717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lucélia Lemes Gonçalves, Anuradha Prakki, Tânia Mara da Silva, Arwa Bafail, Janaína Bortolatto, Alexander Terry Stavroullakis, Sérgio Eduardo de Paiva Gonçalves
{"title":"Longitudinal bond strength of a universal adhesive and chemical dentin characterization under different acid etching protocols.","authors":"Lucélia Lemes Gonçalves, Anuradha Prakki, Tânia Mara da Silva, Arwa Bafail, Janaína Bortolatto, Alexander Terry Stavroullakis, Sérgio Eduardo de Paiva Gonçalves","doi":"10.1590/1678-7757-2023-0359","DOIUrl":"10.1590/1678-7757-2023-0359","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to analyze the longitudinal bond strength of a universal adhesive and chemically characterize the dentin substrate under different acid etching protocols.</p><p><strong>Methodology: </strong>Dentin samples were etched with polyacrylic acid 25% (PAA) for 10 seconds (n=3) and phosphoric acid 32% (PA) for 15 seconds (n=3) and analyzed by Fourier transform infrared spectroscopy - attenuated total reflectance (FTIR-ATR) before and after treatment. For collagen degradation, samples (n=12) were divided into 3 groups: PAA, PA, and Deionized water (control), and analyzed by the quantity of solubilized type I collagen C-terminal cross-linked telopeptides and solubilized C-terminal peptide in relation to total protein concentration (ICTPtp and CTXtp) and by their ultimate tensile strength (UTS). For the adhesive interface analysis, dentin samples (n=72) were divided into 3 groups: PAA, PA, and Self-etch (SE), and subdivided into 2 groups: 24 h (baseline) and 1 year. The following tests were performed: microtensile bond strength (μTBS) (n=48), scanning electron microscopy (SEM) (n=12), and nanoleakage (n=12).</p><p><strong>Results: </strong>The FTIR of PAA showed lower reduction of the peaks in the phosphate group when compared to PA. For ICTPtp, PA showed a significantly higher value. For CTXtp, PA and PAA groups failed to statically differ from each other. UTS was significantly lower for PA. For μTBS, storage time significantly affected bond strength. The results were unaffected by the etching protocol. For SEM, after 1 year, PA had little evidence of degradation in the upper third of the adhesive interface in comparison to the other groups. Nanoleakage showed no considerable silver impregnation after 1 year in the SE group.</p><p><strong>Conclusion: </strong>The use of PAA prior to a universal adhesive (when compared to PA) represents a less aggressive type of etching to dentin. However, self-etching still seems to be the best option for universal adhesive systems that have functional monomers in their composition.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"32 ","pages":"e20230359"},"PeriodicalIF":2.7,"publicationDate":"2024-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11037927/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140305722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Alyssa Teixeira Obeid, Tatiana Rita de Lima Nascimento, Ana Carolina Agassi, Ana Zélia Falcão Almeida, Ana Paula de Melo Alves Guedes, João Marco Alves, Juliana Fraga Soares Bombonatti, Marilia Mattar de Amoêdo Campos Velo
{"title":"Niobium oxyhydroxide as a bioactive agent and reinforcement to a high-viscosity bulk-fill resin composite.","authors":"Alyssa Teixeira Obeid, Tatiana Rita de Lima Nascimento, Ana Carolina Agassi, Ana Zélia Falcão Almeida, Ana Paula de Melo Alves Guedes, João Marco Alves, Juliana Fraga Soares Bombonatti, Marilia Mattar de Amoêdo Campos Velo","doi":"10.1590/1678-7757-2023-0278","DOIUrl":"10.1590/1678-7757-2023-0278","url":null,"abstract":"<p><strong>Objective: </strong>The present in vitro study incorporated niobium oxyhydroxide fillers into an experimental high-viscosity bulk-fill resin composite to improve its mechanical performance and provide it a bioactive potential.</p><p><strong>Methodology: </strong>Scanning electron microscopy synthesized and characterized 0.5% niobium oxyhydroxide fillers, demonstrating a homogeneous morphology that represented a reinforcement for the feature. Fillers were weighed, gradually added to the experimental resin composite, and homogenized for one minute, forming three groups: BF (experimental high-viscosity bulk-fill resin composite; control), BF0.5 (experimental high-viscosity bulk-fill resin composite modified with 0.5% niobium oxyhydroxide fillers), and BFC (commercial bulk-fill resin composite Beautifil Bulk U, Shofu; positive control). In total, 10 specimens/groups (8 × 2 × 2 mm) underwent flexural strength (FS) tests in a universal testing machine (Instron) (500N). Resin composites were also assessed for Knoop hardness (KH), depth of cure (DoC), degree of conversion (DC), elastic modulus (E), and degree of color change (ΔE). The bioactive potential of the developed resin composite was evaluated after immersing the specimens into a simulated body fluid in vitro solution and assessing them using a Fourier-transformed infrared spectroscope with an attenuated total reflectance accessory. One-way ANOVA, followed by the Tukey's test (p<0.05), determined FS, DC, KH, and ΔE. For DoC, ANOVA was performed, which demonstrated no significant difference between groups (p<0.05).</p><p><strong>Conclusions: </strong>The high-viscosity bulk-fill resin composite with 0.5% niobium oxyhydroxide fillers showed promising outcomes as reinforcement agents and performed well for bioactive potential, although less predictable than the commercial resin composite with Giomer technology.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"32 ","pages":"e20230278"},"PeriodicalIF":2.7,"publicationDate":"2024-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11018299/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140305741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}