Journal of Applied Oral Science最新文献

{"title":"Survival rate of the Hall technique compared with resin composite restoration in multi-surface cavities in primary teeth: a 1-year randomized clinical trial.","authors":"Aline Maquiné Pascareli-Carlos,&nbsp;Tamara Kerber Tedesco,&nbsp;Ana Flávia Bissoto Calvo,&nbsp;Isabela Floriano,&nbsp;Thais Gimenez,&nbsp;Monicque da Silva Gonçalves,&nbsp;Daniela Calumby,&nbsp;José Carlos Pettorossi Imparato","doi":"10.1590/1678-7757-2023-0048","DOIUrl":"10.1590/1678-7757-2023-0048","url":null,"abstract":"<p><strong>Background: </strong>Hall technique (HT) has been indicated for teeth with dentinal caries lesion; however, extensive cavities, with more than two surfaces still seem challenging for restorative treatment in pediatric dentistry, resulting in a higher failure rate and an increased need for retreatment.</p><p><strong>Objectives: </strong>To compare the survival rate of the Hall technique preformed metal crown (HT) with resin composite restoration (RC) for multi-surface cavitated caries lesions in primary molars.</p><p><strong>Methodology: </strong>In this multicenter two-arm randomized clinical trial, children between 4 and 9 years of age with at least one primary molar with cavitated caries lesion involving more than two surfaces, including one buccal or palatal/lingual surface, were selected from 17 Brazilian cities. A total of 364 teeth were allocated into two groups: (1) teeth treated with selective caries removal and RC and (2) treated with the HT. The survival rate was assessed at 6 and 12 months after the interventions. Survival analysis was performed with the Kaplan‒Meier method. Cox regression was used to determine the influence of explanatory variables on the survival rate (α=5%).</p><p><strong>Results: </strong>After 12 months, 292 teeth were re-evaluated. A total of 358 teeth were re-evaluated at least once during the study and included in the survival analysis. The HT (87.8%) resulted in a higher survival rate than RC restoration (75.7%) (p=0.004).</p><p><strong>Conclusion: </strong>HT has a higher survival rate than RC as a treatment for multi-surface cavitated caries lesions in primary teeth. ClinicalTrials.gov: NCT02782390.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"31 ","pages":"e20230048"},"PeriodicalIF":2.7,"publicationDate":"2023-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10561961/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41201932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of daily usage times on patients' compliance during at-home bleaching: a randomized clinical trial.","authors":"Caio César Pavani,&nbsp;Ticiane Cestari Fagundes,&nbsp;Daniel Sundfeld,&nbsp;Gabriela Cristina Santin,&nbsp;Lucas Silveira Machado,&nbsp;André Pinheiro de Magalhães Bertoz,&nbsp;Timm Cornelius Schott,&nbsp;Renato Herman Sundfeld","doi":"10.1590/1678-7757-2023-0181","DOIUrl":"10.1590/1678-7757-2023-0181","url":null,"abstract":"<p><p>The effectiveness of at-home dental bleaching treatments depends on the time that bleaching products are in contact with the teeth surface and, consequently, on the adequate use of associated custom acetate trays.</p><p><strong>Objective: </strong>This randomized single-blinded trial aimed to analyze if the daily usage time of these products influences the patient's compliance behavior when submitted to monitored at-home dental bleaching. Secondary outcomes were color change and tooth sensitivity.</p><p><strong>Methodology: </strong>Sixty-six volunteers were randomly distributed into three groups (n=22): patients were instructed to use the trays for 2 (G2), 4 (G4), and 8 (G8) hours daily. The daily dental bleaching compliance behavior was measured using a microsensor inserted into the trays. Subjective and objective color evaluation assessments were adopted at baseline (T0), one (T1), two (T2), and three weeks (T3) after the beginning of the bleaching treatment, as well as two weeks after the treatment (T4). Tooth sensitivity was analyzed using the VAS scale, ranging from T1 to T4.</p><p><strong>Results: </strong>G2 showed a greater degree of cooperation than G8 and cooperation was inversely proportional to the recommended usage time. Significantly higher color change was observed in the upper arch for G8 when compared to G2 in subjective analysis, from T1 to T4. There were no statistical differences between the groups in objective analysis.</p><p><strong>Conclusion: </strong>Shorter recommended usage time of the bleaching product may improve the patient's compliance with at-home dental bleaching treatments. However, increased daily usage time may promote better subjective color change. Bleaching sensitivity was more significant in the first week for a longer time of use.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"31 ","pages":"e20230181"},"PeriodicalIF":2.7,"publicationDate":"2023-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10519669/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41201930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interleukin-17 plays a role in dental pulp inflammation mediated by zoledronic acid: a mechanism unrelated to the Th17 immune response?","authors":"Anna Clara Aragão Matos Carlos,&nbsp;José Vitor Mota Lemos,&nbsp;Marcela Maria Fontes Borges,&nbsp;Maria Carolina Portela Albuquerque,&nbsp;Fabrício Bitu Sousa,&nbsp;Ana Paula Negreiros Nunes Alves,&nbsp;Thinali Sousa Dantas,&nbsp;Paulo Goberlânio de Barros Silva","doi":"10.1590/1678-7757-2023-0230","DOIUrl":"10.1590/1678-7757-2023-0230","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the influence of RORγT inhibition by digoxin on inflammatory changes related to interleukin-17 (IL-17) in the pulp of rats treated with zoledronate (ZOL).</p><p><strong>Methodology: </strong>Forty male Wistar rats were divided into a negative control group (NCG) treated with saline solution, a positive control group (PCG) treated with ZOL (0.20 mg/kg), and three groups treated with ZOL and co-treated with digoxin 1, 2, or 4 mg/kg (DG1, 2, and 4). After four intravenous administrations of ZOL or saline solution in a 70-day protocol, the right molars were evaluated by histomorphometry (number of blood vessels, blood vessels/µm2, cells/µm2, total blood vessel area, and average blood vessel area) and immunohistochemistry (IL-17, TNF-α, IL-6, and TGF-β). The Kruskal-Wallis/Dunn test was used for statistical analysis.</p><p><strong>Results: </strong>PCG showed an increase in total blood vessel area (p=0.008) and average blood vessel area (p=0.014), and digoxin treatment reversed these changes. DG4 showed a reduction in blood vessels/µm2 (p<0.001). In PCG odontoblasts, there was an increase in IL-17 (p=0.002) and TNF-α (p=0.002) immunostaining, and in DG4, these changes were reversed. Odontoblasts in the digoxin-treated groups also showed an increase in IL-6 immunostaining (p<0.001) and a reduction in TGF-β immunostaining (p=0.002), and all ZOL-treated groups showed an increase in IL-17 (p=0.011) and TNF-α (p=0.017) in non-odontoblasts cells.</p><p><strong>Conclusion: </strong>ZOL induces TNF-α- and IL-17-dependent vasodilation and ectasia, and the classical Th17 response activation pathway does not seem to participate in this process.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"31 ","pages":"e20230230"},"PeriodicalIF":2.7,"publicationDate":"2023-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10567106/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41201931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anticancer effects of gossypetin from Hibiscus sabdariffa in oral squamous cell carcinoma.","authors":"Ke Huang,&nbsp;Zhibin Liu,&nbsp;Myoung-Ok Kim,&nbsp;Ki-Rim Kim","doi":"10.1590/1678-7757-2023-0243","DOIUrl":"10.1590/1678-7757-2023-0243","url":null,"abstract":"<p><strong>Objective: </strong>Gossypetin, isolated from Hibiscus sabdariffa L, has been shown to have various pharmacological effects including anti-inflammatory and antibacterial activity against various diseases. However, since the effect of gossypetin in oral cancer remains to be reported, we aimed to investigate the anticancer activity and mechanisms of gossypetin in oral squamous cell carcinoma (OSCC).</p><p><strong>Methodology: </strong>The proliferation of OSCC cells was evaluated by cell viability and soft agar colony assays. The effects of gossypetin on the migration and invasion of OSCC cells was investigated by wound healing and transwell invasion assays, respectively. Apoptosis and cell cycle arrest were measured by flow cytometry. Moreover, the anticancer mechanism of gossypetin in OSCC cells was analyzed by western blotting.</p><p><strong>Results: </strong>Gossypetin inhibited the proliferation, migration, and invasion of OSCC cells and induced apoptosis by upregulating the Bax/Bcl-2 ratio and cell cycle arrest at the G2/M phase. Furthermore, gossypetin regulated the activation of extracellular signal-regulated kinase and nuclear factor-kappa B.</p><p><strong>Conclusion: </strong>Results showed that gossypetin inhibits the proliferation, migration, and invasion of OSCC cells and triggers apoptosis and cell cycle arrest in OSCC. Therefore, gossypetin has the potential for use as a chemopreventive agent in oral cancer.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"31 ","pages":"e20230243"},"PeriodicalIF":2.7,"publicationDate":"2023-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10561964/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41201914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Divergence between confidence and knowledge of endodontists regarding non-odontogenic pain.","authors":"Marcos Dezotti Luiz,&nbsp;Letycia Accioly Simões Coelho,&nbsp;Rodrigo Ricci Vivan,&nbsp;Marco Antônio Hungaro Duarte,&nbsp;Murilo Priori Alcalde,&nbsp;Paulo César Rodrigues Conti,&nbsp;Yuri Martins Costa,&nbsp;Leonardo Rigoldi Bonjardim","doi":"10.1590/1678-7757-2023-0222","DOIUrl":"10.1590/1678-7757-2023-0222","url":null,"abstract":"<p><strong>Introduction: </strong>This study aimed to assess the self-reported levels of confidence and knowledge related to non-odontogenic pain among a group of Brazilian endodontists.</p><p><strong>Methodology: </strong>A total of one hundred and forty-six endodontists affiliated with the Brazilian Society of Endodontics participated in the survey. The questionnaire, distributed via email or WhatsApp, contained inquiries designed to gauge self-perceived confidence and knowledge concerning non-odontogenic pain. The practitioners were categorized into four groups based on their self-reported familiarity with various orofacial pain types, classified as either sufficient or insufficient, and on their engagement in ongoing educational programs related to orofacial pain. Data were analyzed by Chi-Square Test and Fischer's exact test (p<0.05).</p><p><strong>Results: </strong>Overall, self-reported confidence about non-odontogenic pain was high, especially for endodontists who considered their knowledge about orofacial pain sufficient, regardless of whether they had (71.1% - 97.8%) or not (35.7% - 96.4%) been continuously involved in education courses on orofacial pain. In general, self-reported knowledge about non-odontogenic pain was insufficient (0% - 42%), except in the question about how they would act in cases of pain that persists beyond the normal healing time after an endodontic procedure (70.6% - 81.9%). In general, endodontists are confident in their diagnosis and treatment of non-odontogenic pain. Nonetheless, this confidence did not correlate with a commensurate knowledge depth of. Thus, specialization courses in endodontics should highly consider training and qualifying these professionals in the diagnosis of non-odontogenic pain.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"31 ","pages":"e20230222"},"PeriodicalIF":2.7,"publicationDate":"2023-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10561960/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41201915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Obesity influences the development of bisphosphonate-induced osteonecrosis in Wistar rats.","authors":"Wilson José de Miranda Lima,&nbsp;Jannerson Cesar Xavier de Pontes,&nbsp;Ludmila Silva de Figueiredo,&nbsp;Rubens da Silva Araújo,&nbsp;Maria Carolina de Paiva Sousa,&nbsp;Jailane de Souza Aquino,&nbsp;Ricardo Dias de Castro,&nbsp;Adriano Francisco Alves","doi":"10.1590/1678-7757-2023-0133","DOIUrl":"https://doi.org/10.1590/1678-7757-2023-0133","url":null,"abstract":"<p><p>Medication-related osteonecrosis of the jaw (MRONJ) is characterized by bone exposure for more than eight weeks in patients who have used or been treated with antiresorptive or antiangiogenic drugs, without a history of radiation therapy or metastatic diseases in the jaws. Obesity is associated with changes in periodontal tissues and oral microbiota that are linked to bone alterations. This study aimed to analyze the influence of obesity on the development of bisphosphonate-induced osteonecrosis. The experiment randomly and simply divided 24 male Wistar rats (Rattus norvegicus) into four groups: healthy, with osteonecrosis, obese, and obese with osteonecrosis (n=6 per group). Osteonecrosis was induced through weekly intraperitoneal injection for eight weeks at a dose of 250 µg/kg of zoledronic acid in a 4 mg/5 mL solution, combined with trauma (exodontia). Obesity was induced through a high glycaemic index diet. Each group was qualitatively and quantitatively evaluated regarding the development of models and pathological anatomy of the lesions. The results were expressed in mean percentage and standard deviation and statistically analyzed using one-way analysis of variance (ANOVA) followed by Tukey's post-hoc test, with a significance level of 5% (p<0.05) to establish differences found between the groups. Animals in the osteonecrosis group and the obese with osteonecrosis group presented larger necrosis areas (averages: 172.83±18,19 µm2 and 290.33±15,77 µm2, respectively) (p<0,0001). Bone sequestration, hepatic steatosis, and increased adipocyte size were observed in the obese group (average: 97.75±1.91 µm2) and in the obese with osteonecrosis group (average: 98.41±1.56 µm2), indicating greater tissue damage in these groups (p<0,0001). All parameters analyzed (through histological, morphometric, and murinometric analyses) increased for the obese and obese with osteonecrosis groups, suggesting a possible influence of obesity on the results. However, further studies are needed to confirm the role of obesity in the possible exacerbation of osteonecrosis and understand the underlying mechanisms.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"31 ","pages":"e20230133"},"PeriodicalIF":2.7,"publicationDate":"2023-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10547385/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41131153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between nutritional status and children and adolescents' dental caries experiences: an overview of systematic reviews.","authors":"Thaís de Oliveira Fernandes,&nbsp;Patrícia Arriaga Carvalho,&nbsp;Fernanda Volpe de Abreu,&nbsp;Christian Kirschneck,&nbsp;Erika Calvano Küchler,&nbsp;Leonardo Santos Antunes,&nbsp;Lívia Azeredo Alves Antunes","doi":"10.1590/1678-7757-2023-0138","DOIUrl":"10.1590/1678-7757-2023-0138","url":null,"abstract":"<p><p>An increasing number of systematic reviews (SR) has investigated the association between dental caries and nutritional status in children and adolescents, thus requiring an overview to compile the information in a single piece of evidence. Therefore, this study aimed to evaluate and summarize evidence from published SR on the association between dental caries and nutritional status in children and adolescents. A wide search was conducted on May 29, 2023, in six databases (Medline via PubMed, Scopus, Web of Science, Cochrane library, Embase, and the Virtual Health Library - VHL). An additional search was performed in the gray literature (Open grey and Google Scholar), SR registration databases, and the list of references of the included SR. Our inclusion criteria were based on acronym PECOS. Overall, two reviewers independently extracted the data, evaluated the risk of bias (ROBIS), and assessed the quality of the chosen studies (AMSTAR-2). Data from the included meta-analysis were summarized and certainty of evidence using the GRADE approach was performed. After removing duplicates and applying our eligibility criteria, 19 SR from 2006-2022 were included. We found that 17 SR showed high risk of bias and critically low methodological quality. We observed an association between dental caries experiences and nutritional status since seven SR found an association between obesity/overweight and dental caries; one, an association between underweight and dental caries; and eleven, no associations. The meta-analysis showed divergent results according to the study designs, used indices, and participants' age group, and were scored as having a very low certainty of evidence. Therefore, based on the high risk of bias, low methodological quality, and very low certainty of evidence of the chosen SR, most studies found no association between children and adolescents' nutritional status and dental caries experience.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"31 ","pages":"e20230138"},"PeriodicalIF":2.7,"publicationDate":"2023-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10561963/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41144668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating the association between dental age and polymorphisms in genes encoding estrogen receptors.","authors":"Isabela Ribeiro Madalena,&nbsp;Caio Luiz Bitencourt Reis,&nbsp;Mirian Aiko Nakane Matsumoto,&nbsp;Maria Bernadete Sasso Stuani,&nbsp;Natanael Henrique Ribeiro Mattos,&nbsp;Daniela Silva Barroso de Oliveira,&nbsp;Maria Angélica Hueb de Menezes Oliveira,&nbsp;Liliane Roskamp,&nbsp;Erika Calvano Küchler,&nbsp;Flares Baratto-Filho","doi":"10.1590/1678-7757-2023-0184","DOIUrl":"10.1590/1678-7757-2023-0184","url":null,"abstract":"<p><strong>Background: </strong>Genetic polymorphisms have been shown to influence several physiological traits, including dental and craniofacial characteristics. Understanding the clinical relevance of genetic polymorphisms in dental practice is crucial to personalize treatment plans and improve treatment outcomes.</p><p><strong>Objective: </strong>to evaluate the association between dental age and genetic polymorphisms in genes encoding estrogen receptors alpha and beta (ESR1 and ESR2, respectively) in a sample of Brazilian children.</p><p><strong>Methodology: </strong>This retrospective cross-sectional study was performed with children undergoing orthodontic treatment. Patients with syndromes, congenital anomalies, craniofacial deformities, under hormonal or systemic treatment, and with a previous history of facial trauma were excluded. Panoramic radiographs were used to assess dental age according to the Demirjian, Goldstein, and Tanner method. A delta [dental age-chronological age (DA-CA)] was obtained, which shows whether the patient tends to have a normal, delayed (negative values), or advanced (positive values) dental age. DNA isolated from buccal cells was used to genotype four genetic polymorphisms: rs9340799 (A>G) and rs2234693 (C>T), located in ESR1; and rs1256049 (C>T) and rs4986938 (C>T), located in ESR2. A statistical analysis was performed and values of p<0.05 indicated statistical difference.</p><p><strong>Results: </strong>A total of 79 patients were included, 44 (55.70%) girls and 35 (44.30%) boys. The Demirjian, Goldstein, and Tanner method, in general, overestimated patients' age by 0.75 years. There was no difference in the delta of dental age between the sexes (p>0.05). Genetic polymorphisms in ESR1 and ESR2 were not associated with dental age (p>0.05).</p><p><strong>Conclusion: </strong>The studied genetic polymorphisms in ESR1 and ESR2 were not associated with dental age in Brazilian children.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"31 ","pages":"e20230184"},"PeriodicalIF":2.7,"publicationDate":"2023-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10547381/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41137607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Does radiation therapy affect adhesion of tricalcium silicate cements to root dentin?","authors":"Lochan Khullar,&nbsp;Nidambur Vasudev Ballal,&nbsp;Tan Fırat Eyüboğlu,&nbsp;Mutlu Özcan","doi":"10.1590/1678-7757-2023-0118","DOIUrl":"https://doi.org/10.1590/1678-7757-2023-0118","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to analyze the effect of irradiation on the push-out bond strength of mineral trioxide aggregate (MTA) and Biodentine to radicular dentin.</p><p><strong>Methodology: </strong>A total of 60 extracted mature human teeth with single root canals were categorized into two groups (irradiated and non-irradiated) (n=30). Each group was further divided into two sub-groups based on cements used (Biodentine and MTA). Then, a cumulative radiation dose of 60 Gy was divided into 30 fractions (two Gy for every fraction) and administered for five successive days per week over six weeks. Obturation was then performed using MTA and Biodentine. Afterwards, 1.5 mm thick horizontal sections were procured from the middle one-third of all the specimens and then subjected to push-out bond test. Results were analyzed using one-way analysis of variance with post-hoc Tukey's test.</p><p><strong>Results: </strong>The bond strength of Biodentine and MTA to irradiated teeth was lower than non-irradiated teeth. Highest push-out bond strength was observed in non-irradiated specimens filled with Biodentine (p=0), followed by irradiated specimens filled with Biodentine (p=0); non-irradiated specimens filled with MTA (p=0); and irradiated specimens filled with MTA (p=0.9).</p><p><strong>Conclusion: </strong>The push-out bond strength of Biodentine and MTA to root canal dentin decreased significantly post irradiation.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"31 ","pages":"e20230118"},"PeriodicalIF":2.7,"publicationDate":"2023-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41115346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Colonization dynamics of subgingival microbiota in recently installed dental implants compared to healthy teeth in the same individual: a 6-month prospective observational study.","authors":"Carina Maciel Silva-Boghossian,&nbsp;Pablo Torquilho Duarte,&nbsp;Denise Gome da Silva,&nbsp;Talita Gomes Baêta Lourenço,&nbsp;Ana Paula Vieira Colombo","doi":"10.1590/1678-7757-2023-0134","DOIUrl":"https://doi.org/10.1590/1678-7757-2023-0134","url":null,"abstract":"<p><strong>Objectives: </strong>To evaluate the colonization dynamics of subgingival microbiota established over six months around newly installed dental implants in periodontally healthy individuals, compared with their corresponding teeth.</p><p><strong>Methodology: </strong>Seventeen healthy individuals assigned to receive single dental implants participated in the study. Subgingival biofilm was sampled from all implant sites and contralateral/ antagonist teeth on days 7, 30, 90, and 180 after implant installation. Microbiological analysis was performed using the Checkerboard DNA-DNA hybridization technique for detection of classical oral taxa and non-oral microorganisms. Significant differences were estimated by Mann-Whitney and Friedman tests, while associations between implants/teeth and target species levels were assessed by linear regression analysis (LRA). Significance level was set at 5%.</p><p><strong>Results: </strong>Levels of some species were significantly higher in teeth compared to implants, respectively, at day 7 ( V.parvula , 6 × 10 5 vs 3 × 105 ; Milleri streptococci , 2 × 10 6 vs 6 × 10 5 ; Capnocytophaga spp., 2 × 10 6 vs 9 × 10 5 ; E.corrodens , 2 × 10 6 vs 5 × 10 5 ; N. mucosa , 2 × 10 6 vs 5 × 10 5 ; S.noxia , 2 × 10 6 vs 3 × 10 5 ; T.socranskii , 2 × 10 6 vs 5 × 10 5 ; H.alvei , 4 × 10 5 vs 2 × 10 5 ; and Neisseria spp., 6 × 10 5 vs 4 × 10 4 ), day 30 ( V.parvula , 5 × 10 5 vs 10 5 ; Capnocytophaga spp., 1.3 × 10 6 vs 6.8 × 10 4 ; F.periodonticum , 2 × 10 6 vs 10 6 ; S.noxia , 6 × 10 5 vs 2 × 10 5 ; H.alvei , 8 × 10 5 vs 9 × 10 4 ; and Neisseria spp., 2 × 10 5 vs 10 6 ), day 120 ( V.parvula , 8 × 10 5 vs 3 × 10 5 ; S.noxia , 2 × 10 6 vs 0; and T.socranskii , 3 × 10 5 vs 8 × 10 4 ), and day 180 ( S.enterica subsp. enterica serovar Typhi, 8 × 10 6 vs 2 × 10 6 ) (p<0.05). Implants showed significant increases over time in the levels of F.nucleatum , Gemella spp., H.pylori , P.micra , S.aureus , S.liquefaciens , and T.forsythia (p<0.05). LRA found that dental implants were negatively correlated with high levels of S. noxia and V. parvula (β=-0.5 to -0.3; p<0.05).</p><p><strong>Conclusions: </strong>Early submucosal microbiota is diverse and only a few species differ between teeth and implants in the same individual. Only 7 days after implant installation, a rich microbiota can be found in the peri-implant site. After six months of evaluation, teeth and implants show similar prevalence and levels of the target species, including known and new periodontopathic species.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"31 ","pages":"e20230134"},"PeriodicalIF":2.7,"publicationDate":"2023-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10519670/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41131515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}