Journal of Applied Oral Science最新文献

{"title":"The systemic effect of prolonged use of sodium alendronate on the peri-implant bone repair process.","authors":"Paula Buzo Frigério, Daniel Silva Leão, Pedro Henrique Silva Gomes-Ferreira, Naara Gabriela Monteiro, Daniele Botticelli, Roberta Okamoto","doi":"10.1590/1678-7757-2025-0085","DOIUrl":"https://doi.org/10.1590/1678-7757-2025-0085","url":null,"abstract":"<p><strong>Background: </strong>Bisphosphonates are the most widely marketed drugs in the world to treat osteoporosis but their anti-remodeling effect can cause osseointegration to fail.</p><p><strong>Objective: </strong>this study aimed to evaluate bone quality during peri-implant repair in osteopenic rats, adopting an approach inverse to the conventional one in which prolonged treatment with alendronate sodium was initiated only after the installation of titanium implants.</p><p><strong>Methodology: </strong>implants were installed in the tibias of 32 rats 14 days after ovariectomy (osteopenia). After 14 days, systemic treatment was initiated by gavage with a saline solution or alendronate. At 28 and 56 days after implantation, the rats received calcein (20 mg/kg, i.m.). They were administered alizarin red (20 mg/kg, i.m.) seven days before euthanasia. Euthanasia occurred in two periods, 42 and 70 post-implantation days.</p><p><strong>Results: </strong>the highest implant removal torques were observed for the OVX ALE at 70 days (13.35 Ncm) and the OVX SAL at 42 days (11.63 Ncm). The expression of bone remodeling and resorption proteins (OPG/ RANKL) was higher in the alendronate-treated animals. In contrast, osteocalcin and bone sialoprotein were similar between the control and alendronate-treated groups. No parameter showed statistically significant variations (BV, BV.TV, Tb.Th, Tb. N, Tb.Sp, and i.S), with similar measurements between groups. Fluorochrome analysis showed active bone remodeling at the implant interface.</p><p><strong>Conclusion: </strong>the alendronate treatment stabilized the bone-implant interface over time, suggesting a protective effect against osteoporotic bone loss despite a delayed initial response. In the long term, the group systemically treated with alendronate showed improved bone formation around the implants, outperforming the control group. However, future research is essential to prevent possible negative impacts on osseointegration in patients taking this medication.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"33 ","pages":"e20250085"},"PeriodicalIF":2.6,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145205911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing the validity of ChatGPT-4o and Google Gemini Advanced when responding to frequently asked questions in endodontics.","authors":"Nicolás Dufey-Portilla, Ana Billik Frisman, Maximiliano Gallardo Robles, Fernando Peña-Bengoa, Consuelo Cabrera Ávila, Venkateshbabu Nagendrababu, Paul M H Dummer, Marc Garcia-Font, Francesc Abella Sans","doi":"10.1590/1678-7757-2025-0321","DOIUrl":"https://doi.org/10.1590/1678-7757-2025-0321","url":null,"abstract":"<p><p>Artificial intelligence (AI) is transforming access to dental information via large language models (LLMs) such as ChatGPT and Google Gemini. Both models are increasingly being used in endodontics as a source of information for patients. Therefore, as developers release new versions, the validity of their responses must be continuously compared to professional consultations.</p><p><strong>Objective: </strong>This study aimed to evaluate the validity of the responses provided by the most advanced LLMs [Google Gemini Advanced (GGA) and ChatGPT-4o] to frequently asked questions (FAQs) in endodontics.</p><p><strong>Methodology: </strong>A cross-sectional analytical study was conducted in five phases. The top 20 endodontic FAQs submitted by users to chatbots and collected from Google Trends were compiled. In total, nine academically certified endodontic specialists with educational roles scored GGA and ChatGPT-4o responses to the FAQs using a five-point Likert scale. Validity was determined using high (4.5-5) and low (≥4) thresholds. The Fisher's exact test was used for comparative analysis.</p><p><strong>Results: </strong>At the low threshold, both models obtained 95% validity (95% CI: 75.1%- 99.9%; p=.05). At the high threshold, ChatGPT-4o achieved 35% (95% CI: 15.4%- 59.2%) and GGA, 40% (95% CI: 19.1%- 63.9%) validity (p=1).</p><p><strong>Conclusions: </strong>ChatGPT-4o and GGA responses showed high validity under lenient criteria that significantly decreased under stricter thresholds, limiting their reliability as a stand-alone source of information in endodontics. While AI chatbots show promise to improve patient education in endodontics, their validity limitations under rigorous evaluation highlight the need for careful professional monitoring.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"33 ","pages":"e20250321"},"PeriodicalIF":2.6,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145206559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Estrogen versus bone marrow mesenchymal stromal cells in the regeneration of the parotid gland in ovariectomized rats.","authors":"Sally Hassan Abo Baker, Amira Ahmed R Moawad","doi":"10.1590/1678-7757-2025-0374","DOIUrl":"https://doi.org/10.1590/1678-7757-2025-0374","url":null,"abstract":"<p><strong>Objectives: </strong>Menopause causes specific hormonal alterations in women that make them more susceptible to salivary changes, such as decreased saliva flow, which can lead to xerostomia, modified taste, and a burning sensation in the mouth. This study aimed to determine which of two treatments-bone marrow-derived mesenchymal stromal cells (BM-MSCs) or estrogen-more effectively ameliorate the postmenopausal degenerative effects on the parotid salivary glands of ovariectomized rats by histological, immunohistochemical, and malondialdehyde (MDA) evaluations.</p><p><strong>Methodology: </strong>The study specimen involved 70 female albino rats. Group I received saline, and were subdivided into sham-operated and vehicle-treated. Group II was subjected to bilateral ovariectomy and received no treatment. Group III was subjected to bilateral ovariectomy and, one week later, treatment with subcutaneous injections of 1 mg/kg/daily estrogen for 12 weeks. Group IV underwent bilateral ovariectomy and, one week later, received a single intraglandular injection of a BM-MSC solution. At the end of the experiment, the rats were euthanized, and their parotid glands were dissected and processed for H&E stain, caspase 3, and MDA evaluations.</p><p><strong>Results: </strong>The histological and immunohistochemical findings in Group II showed marked degenerative changes in the parotid gland. However, in Groups III and IV, regeneration was observed following treatment with estrogen and BM-MSCs, respectively. The estrogen and BM-MSCs groups showed significant decrease in MDA levels in the parotid gland relative to the ovariectomized group and nearly comparable to control.</p><p><strong>Conclusion: </strong>BM-MSCs and estrogen show histological efficacy in regenerating the parotid salivary glands of ovariectomized rats.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"33 ","pages":"e20250374"},"PeriodicalIF":2.6,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145130920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biocompatibility and bioactivity of bioceramic endodontic sealer: NeoSealer Flo.","authors":"Evelin Carine Alves Silva, Jéssica Arielli Pradelli, Guilherme Ferreira da Silva, Paulo Sérgio Cerri, Mario Tanomaru-Filho, Juliane Maria Guerreiro-Tanomaru","doi":"10.1590/1678-7757-2025-0284","DOIUrl":"https://doi.org/10.1590/1678-7757-2025-0284","url":null,"abstract":"<p><strong>Objective: </strong>Bioceramic materials are developed based on their biological properties and bioactive potential. NeoSealer Flo (NeoFlo, NuSmile, Houston) is a ready-to-use bioceramic endodontic sealer. The aim of this study was to evaluate the biocompatibility and bioactivity of NeoFlo compared to Bio-C Sealer (BC, Angelus) and AH Plus (AHP, Dentsply).</p><p><strong>Methodology: </strong>The tissue reaction induced by materials in rat subcutaneous tissues was assessed at seven, 15-, 30-, and 60-days post-implantation (n=6/group). The number of inflammatory cells (ICs), fibroblasts, and osteocalcin (OCN)-labelled cells were recorded. Amorphous calcite was identified using the von Kossa method and polarized light. The data were evaluated using two-way ANOVA followed by Tukey's test, with a 5% significance level. OCN data were submitted to the Kruskal-Wallis test, with Dunn, Friedman, and Nemenyi post-hoc tests.</p><p><strong>Results: </strong>NeoFlo capsules showed higher number of IC than BC and AHP (p<0.05) in all periods, with a reduction over time, and was considered moderate at 60 days. Moreover, significant reduction in the number of IC and an increase in the fibroblasts was accompanied by an increase in the amount of collagen in the capsules around all materials over time. Immunoexpression of OCN was only observed in the capsules of NeoFlo and BC, but the capsules of BC showed the highest values in all periods (p<0.05).</p><p><strong>Conclusion: </strong>In the present study, NeoFlo showed lower biocompatibility than BC, however, NeoFlo shows bioactivity in connective tissue.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"33 ","pages":"e20250284"},"PeriodicalIF":2.6,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145130915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intra-familial phenotype variant in hypoplastic amelogenesis imperfecta under a complex genetic component: a family report, whole-exome sequencing, and literature review.","authors":"Célia Regina Moreira Lanza, Artur Melo Rodrigues, Iasmin Fonseca Tolentino Mascarenhas, Talita Roberta Ferreira de Souza, Matheus Oliveira Reis, Felipe Morando Avelar, Maria Raquel Santos Carvalho, Vasco Ariston Carvalho de Azevedo, Debmalya Barh","doi":"10.1590/1678-7757-2024-0489","DOIUrl":"10.1590/1678-7757-2024-0489","url":null,"abstract":"<p><strong>Background: </strong>Amelogenesis imperfecta (AI) encompasses a group of conditions characterized by abnormalities in the development or function of tooth enamel. Clinical manifestations include different forms and degrees of enamel frailty, associated with sensitivity, tooth fractures, stains, abnormal tooth morphology, missing teeth, etc. AI is genetically heterogeneous, with over 70 genes associated with autosomal dominant, autosomal recessive, X-linked, and oligogenic inheritance.</p><p><strong>Objective: </strong>To identify genetic variants associated with AI in a single family.</p><p><strong>Methodology: </strong>We describe the clinical findings of a family affected by AI, composed of five individuals: four affected (the father and three daughters) and one unaffected (the mother). The observed segregation pattern suggests a dominant, X-linked inheritance. Genetic variants were screened using whole-exome sequencing. The initial bioinformatic analysis was conducted using Qiagen QCI, and variants were selected based on their presence in all four affected family members and absence in the unaffected mother. Search terms included \"amelogenesis imperfecta,\" \"tooth,\" and \"enamel.\" Several types of software were used to classify variants according to pathogenicity.</p><p><strong>Results: </strong>Candidate variants were identified in six genes. Three of these variants were detected in autosomal genes: NM_031889.3(ENAM):c.1726T>C (p.F576L), NM_022168.4(IFIH1):c.1764dupA, (p.A589fs*21), and NM_032383.5(HPS3):c.1897A>T (p.M633L). Three variants were detected in X-linked genes: NM_006150.5(PRICKLE3):c.8C>G (p.A3G), NM_004484.4(GPC3):c.584A>G (p.N195S), and NM_152787.5(TAB3):c.1936G>A (p.V646M). None of these variants were classified as pathogenic or likely pathogenic in AI.</p><p><strong>Discussion: </strong>Among the identified genes, only ENAM has previously been associated with AI; however, IFIH1, PRICKLE3, and GPC3 are associated with dental/enamel development. The relatively high number of candidate genes and variants detected may reflect an oligogenic component already proposed for AI.</p><p><strong>Conclusions: </strong>This study provides a set of new candidate genes and genetic variants for AI. Despite sharing the same variants, AI-affected family members show considerable phenotypic variant, suggesting the involvement of non-shared genetic or environmental factors.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"33 ","pages":"e20240489"},"PeriodicalIF":2.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144955230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Etching on the edge: enamel loss under repeated and active HCl applications as a resin infiltration pretreatment.","authors":"Maria Paula Novaes Camargo Manna, Talita Portela Pereira, Bruna de Oliveira Iatarola, Mariele Vertuan, Ana Carolina Magalhães, Denise Maria Zezell, Luciana Fávaro Francisconi-Dos-Rios","doi":"10.1590/1678-7757-2025-0103","DOIUrl":"10.1590/1678-7757-2025-0103","url":null,"abstract":"<p><strong>Background: </strong>Considering that a single passive application of hydrochloric acid (HCl) as a resin infiltration pretreatment can remove between 20 and 45 µm of enamel and cause etching that extends up to 2 mm beyond the white spot lesions (WSLs), it is plausible that its repeated and active applications could result in a greater amount of dental tissue being removed.</p><p><strong>Objective: </strong>To evaluate the enamel surface loss and micromorphology after etching with 15% HCl using two application methods (passive-P and active-A) and varying numbers of applications (C-placebo - 120 s; 1x HCl - 120 s; 2x HCl - 120 s + 120 s; 3x HCl - 120 s + 120 s + 120 s).</p><p><strong>Methodology: </strong>Bovine incisors with ≤0.3 µm initial curvature were randomized into eight groups (n=12) based on microhardness, followed by WSL simulation. A central window was etched according to experimental conditions, and surface loss was assessed using optical profilometry and micromorphology via scanning electron microscopy (SEM). Two-way ANOVA and Tukey's test were used for surface loss, and the chi-square test evaluated the association of experimental conditions with etching patterns (α=0.05).</p><p><strong>Results: </strong>1xP generated intermediate mean surface loss, positioned between the values observed for passive control (PC) and active control (AC), and those for 2xP and 3xP. Losses from active applications were significantly higher than passive ones and were increased by the number of applications. SEM showed Types II and III etching patterns and Type II was more frequent. There was no association between treatment and etching pattern.</p><p><strong>Conclusion: </strong>Multiple and active HCl applications may raise concerns about removal of the remaining tooth structure, challenging the principles of minimal intervention dentistry.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"33 ","pages":"e20250103"},"PeriodicalIF":2.6,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144955258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trueness comparison of various intraoral scanners and hybrid workflow for ceramic restoration.","authors":"Taksid Charasseangpaisarn, Kritsaya Srimakhajon, Suchanan Sasiwilasakorn, Korravit Hanvivattanakul, Pemika Sangvichit, Kornpavee Simapronchai","doi":"10.1590/1678-7757-2025-0065","DOIUrl":"https://doi.org/10.1590/1678-7757-2025-0065","url":null,"abstract":"<p><strong>Background: </strong>The scanned abutment file for the digital design of restorations can be either obtained directly using the intraoral scanner (IOS) or scanning the impression or the working model with the extraoral scanner (EOS). The trueness of the scanned file pertains to its effect on the accuracy of the restoration.</p><p><strong>Objective: </strong>This study aimed to compare the trueness of scan files from different intraoral scanners (IOSs) and the hybrid workflow using the E3 extraoral scanner (EOS) for ceramic restoration.</p><p><strong>Methodology: </strong>The model of the mandibular right first molar was prepared for the ceramic crown, and it was scanned with the EOS in reference Standard Tessellation Language (STL) file format. The following seven experimental groups were investigated. The IOSs-iTero Element 5D (IT), Trios 4 (TF), Medit i700 (MI), Primescan (PM), Virtuo Vivo (VV)-were directly scanned on the prepared model. The silicone impression of the prepared model was scanned with EOS (IS). The working model poured from the impression was scanned with the EOS (WS). The test STL file was trimmed and superimposed on the reference STL file for the trueness assessment using Geomagic Control X. The point deviation at the surface and margin of each group were compared. The mean deviation was calculated and statistically analyzed with One-way ANOVA (α=0.05). The minimum and maximum deviation of each area were also recorded.</p><p><strong>Results: </strong>Compared with the other groups, the impression scan group had a significantly greatest deviation (p<0.05) in surface (37.65±1.14 µm), margin (63.57±5.85 µm) and overall (50.61±3.28 µm). The WS group showed significantly greater deviation (p<0.05) in surface (23.93±1.20 µm), margin (46.18±2.00 µm) and overall (35.05±1.16 µm) than the IOS groups. In some IOS groups, the deviation was also significantly different (p<0.05).</p><p><strong>Conclusion: </strong>The IOS is recommended for obtaining the scanned file due to its lesser deviation when compared to the hybrid workflow. While statistical differences exist among IOSs, the clinical relevance of these differences appears minimal. If the IOS does not exist, scanning the working model is preferred over scanning impressions directly. However, further clinical validation studies are necessary to confirm this finding.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"33 ","pages":"e20250065"},"PeriodicalIF":2.6,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144955265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PI3K/AKT signaling mediate collagen type 1-induced osteogenic differentiation of dental pulp stem cells via focal adhesion mechanism.","authors":"Nur Julia Nabila Nasir, Norsyahida Arifin, Khairul Bariah Ahmad Amin Noordin, Norhayati Yusop","doi":"10.1590/1678-7757-2025-0173","DOIUrl":"10.1590/1678-7757-2025-0173","url":null,"abstract":"<p><strong>Background: </strong>Dental pulp stem cells (DPSCs) are widely available sources of stem cells that have been extensively studied for its capacity to differentiate into osteoblasts and endothelial cells and to support bone repair and regeneration. Collagen type 1 (Col-1) is a well-known extracellular matrix component, which plays a vital role in regulating the signaling pathway for osteoinduction of bone progenitor cells. However, the exact mechanism of Col-1 activation during stem cell osteogenesis remains unclear.</p><p><strong>Objectives: </strong>This study aims to identify the key signalling pathway and proteins interaction associated with Col-1-induced osteogenesis of DPSCs.</p><p><strong>Methodology: </strong>The localization of OCN protein was assessed by immunocytochemistry analysis, followed by Western blot analysis on OCN, AKT, p- AKT, Smad2/3, p-Smad2/3, ERK1/2, and p-ERK1/2 pathways. Protein profiling was performed using gel-free digestion and LC-MS/MS, followed by protein-protein interaction analysis using STRING online tools to assist in determination of link between various pathways.</p><p><strong>Results: </strong>The data indicated that the PI3K/AKT pathway is the key signaling pathway involved in Col-1-induced DPSC, showing a significant impact and potential crosstalk with TGF-b/Smad and MAPK/ERK mainly via focal adhesion protein complexes.</p><p><strong>Conclusion: </strong>The evidence suggests that PI3K/AKT signaling pathway is more dominant than the TGF-β/Smad and MAPK/ERK pathways, acting via stimulation of the focal adhesion protein complex. Together, these findings may provide deeper insight into cellular biology of differentiated cells for potential manipulation in bone tissue repair and regeneration.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"33 ","pages":"e20250173"},"PeriodicalIF":2.6,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144955182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antioxidant potential of wine polyphenols on hematological indices and apical periodontitis in male rats.","authors":"Romulo de Oliveira Sales-Junior, Julissa Denisse Arguello Alvarado, Bharbara de Moura Pereira, Rafaela Ricci, Nathália Evelyn da Silva Machado, Murilo Catelani Ferraz, Rayara Nogueira de Freitas, Renan Jose Barzotti, Antonio Hernandes Chaves-Neto, Luciano Tavares Ângelo Cintra, João Eduardo Gomes-Filho","doi":"10.1590/1678-7757-2025-0229","DOIUrl":"10.1590/1678-7757-2025-0229","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the effects of dealcoholized red wine supplementation on blood cells and the redox state in male rats with established apical periodontitis (AP).</p><p><strong>Methodology: </strong>Thirty-two male Wistar rats were assigned to one of four groups: control (C), dealcoholized red wine (DRW), red wine (RW), and alcohol (AL). AP was induced, and supplementation was administered for 30 days, starting 30 days after AP induction. At the end of the 60th day, the maxillae were removed for AP radiographic analysis and blood was collected for blood cell and redox state analysis. Statistical tests were applied (p<0.05).</p><p><strong>Results: </strong>The C and DRW groups showed higher weight gain percentages (p<0.05). The DRW and AL groups exhibited the smallest and the largest periapical lesion areas, respectively (p<0.05). The RW and DRW groups showed similar red blood cell parameters to the C group but different from the AL group (p<0.05). Lymphocyte counts were smaller in the DRW and RW groups compared to the AL and C groups (p<0.05), and the neutrophil count was lower in the AL group (p<0.05). No significant differences were found in monocytes and in lipid and protein oxidative damage. Superoxide dismutase activity was lower in the AL group (p<0.05). The DRW group presented a higher glutathione concentration compared to the RW and AL groups (p<0.05).</p><p><strong>Conclusion: </strong>DRW reduced periapical lesion size and altered the blood profile by reducing the lymphocyte count and increasing the concentration of endogenous antioxidants such as GSH in male rats with established AP.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"33 ","pages":"e20250229"},"PeriodicalIF":2.6,"publicationDate":"2025-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144835178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Patient compliance with braces vs. Invisalign®: a secondary data analysis from a randomized clinical trial.","authors":"Graziela Hernandes Volpato, Paula Vanessa Pedron Oltramari, Renata Rodrigues de Almeida-Pedrin, Thais Maria Freire Fernandes, Marcio Rodrigues de Almeida, Gabriela Utrago, João Poiani, Ana Cláudia de Castro Ferreira Conti","doi":"10.1590/1678-7757-2025-0179","DOIUrl":"10.1590/1678-7757-2025-0179","url":null,"abstract":"<p><p>The success of orthodontic treatment depends on several factors that may influence the desired outcome, such as patient motivation and cooperation.</p><p><strong>Objective: </strong>This study compared patient compliance during the first year of orthodontic treatment using aligners and conventional fixed appliances.</p><p><strong>Methodology: </strong>The sample included 39 participants (22.1±4.62 years, 25 males, 14 females) randomized into two groups: orthodontic aligners (OA, 20 patients, 23.7±5.6 years, 12 males, 8 females) and fixed appliances (FA, 19 patients, 20.5±4.5 years, 12 males, 7 females). Patient compliance was measured using the Orthodontic Patient Cooperation Scale (OPCS) composed of 10 questions related to attitudes and attendance of patients in relation to treatment. The questionnaire was applied at three different points: at 3 months (T1), at 6 months (T2), and at 12 months (T3) of treatment. The psychosocial profile of the patients was assessed using the anxiety (IDATE) and stress (PSS-14) questionnaires. Both groups were comparable in age, gender and psychosocial profile. Compliance comparisons between groups at T1, T2 and T3, as well as between genders, were performed using the Mann-Whitney test. Comparison between T1, T2 and T3 were performed using the Friedman test. The correlation between age and compliance scores was assessed using the Spearman's correlation coefficient. The psychosocial profile was analyzed using the Independent t-test. All tests considered p<0.05.</p><p><strong>Results: </strong>The type of appliance (OA or FA) and the time of assessment did not significantly influence patient compliance. The age and gender of patients were not correlated with their degree of compliance. Patient compliance was similar in the first 12 months of treatment, regardless of the protocol used, the patient's gender and age.</p><p><strong>Conclusions: </strong>The level of patient compliance with orthodontic treatment was not influenced by the type of appliance (conventional fixed appliances or aligners), nor by the patient's age or gender.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"33 ","pages":"e20250179"},"PeriodicalIF":2.6,"publicationDate":"2025-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144835190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}