Journal of Applied Oral Science最新文献

{"title":"Histological findings in post-extraction sockets after alveolar ridge preservation with leukocyte- and platelet-rich fibrin: a randomized clinical trial.","authors":"Maria Pavli, Maria Georgaki, Nadia Theologie-Lygidakis, Nikolaos Nikitakis, Christos Perisanidis","doi":"10.1590/1678-7765-2026-0283","DOIUrl":"10.1590/1678-7765-2026-0283","url":null,"abstract":"<p><strong>Introduction: </strong>Leukocyte- and platelet-rich fibrin (L-PRF) has gained considerable attention due to its regenerative potential and biocompatibility in alveolar ridge preservation (ARP).</p><p><strong>Objective: </strong>To evaluate the histological findings in post-extraction sockets after ARP with L-PRF (experimental group) and compare them with those obtained using allograft plus a collagen membrane (control group). Histological findings may contribute to the evaluation of bone quality and the rate of bone remodelling.</p><p><strong>Methodology: </strong>Twenty-six sites in 26 patients requiring extraction of a single-rooted maxillary or mandibular tooth were included in this study. Treatment options were randomly assigned. Post-extraction sockets were filled with either an L-PRF matrix or an allograft covered with a collagen membrane. Dental implant placement was performed subsequently. After 12 weeks, bone biopsies were obtained from the sites at the time of implant placement. Histological evaluation of the bone specimens assessed the area of new bone formation and the presence of osteoblasts, osteoclasts, osteolytic lacunae, osteocytes, and blood vessels.</p><p><strong>Results: </strong>Histological evaluation showed that: a) the proportion of cases with >50% new bone formation was higher in the experimental group compared to the control group (53.8% [7/13] versus 30.8% [4/13]) (p=0.033) b) the presence of osteoblasts was higher (p=0.066) in the experimental group (100% versus 76.9%); c) the presence of osteoclasts was higher (p=0.004) in the experimental group (92.3% versus 38.5%); d) the presence of osteolytic lacunae was higher (p=0.063) in the experimental group (92.3% versus 61.5%); e) the presence of osteocytes was higher (p=0.03) in the experimental group (100% versus 69.2%); and f) the presence of blood vessels was higher (p=0.016) in the experimental group (84.6% versus 38.5%). The presence of osteoblasts and osteolytic lacunae were higher but were not statistically significant.</p><p><strong>Conclusions: </strong>Within the limitations of this study, ARP with L-PRF may result in accelerated healing, improved bone quality, and a higher rate of bone remodelling. These results reflect the exploratory nature of this study and support the need of additional research, preferably with a larger sample size.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"34 ","pages":"e20260283"},"PeriodicalIF":3.0,"publicationDate":"2026-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13668467/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149110182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A dual-scale micro/nanofibrous PLGA scaffold fabricated by co-electrospinning for regenerative endodontic procedures.","authors":"Stephanie Isabel Diaz Zamalloa, Caroline Carvalho Dos Santos, Leticia Martins Santos, Victor Elias Arana-Chavez, Fernando Neves Nogueira, Carla Renata Sipert, Celso Luiz Caldeira","doi":"10.1590/1678-7765-2026-0524","DOIUrl":"10.1590/1678-7765-2026-0524","url":null,"abstract":"<p><strong>Introduction: </strong>The unpredictability of blood clots as natural scaffolds in regenerative endodontic procedures (REPs) has driven the search for synthetic alternatives with reproducible properties. This study aimed to develop and characterize a dual-scale micro/nanofibrous poly(lactic-co-glycolic acid) (PLGA) scaffold fabricated by co-electrospinning and evaluate its physicochemical and biological suitability for REPs under lipopolysaccharide (LPS)-induced inflammatory conditions.</p><p><strong>Methodology: </strong>A 7.5% (w/v) PLGA solution was co-electrospun using distinct parameters (flow rate and needle-to-collector distance) to generate a dual-scale fiber architecture. Physicochemical characterization included water uptake, in vitro degradation, and morphological analysis by scanning electron microscopy (SEM). Biological properties were evaluated using human apical papilla cells (APCs). Metabolic activity (Alamar Blue), mineralization (Alizarin Red S), and cell adhesion/morphology (SEM) were assessed in the presence or absence of LPS to simulate an inflammatory environment.</p><p><strong>Results: </strong>The co-electrospun scaffold showed a hierarchical structure with interconnected micro and nanofibers. Water uptake reached 147.4% within 24 h and increased gradually thereafter. The scaffold showed a biphasic degradation profile-slow initial degradation (21.5% over 28 days) followed by accelerated loss (63.1% at day 45). Hydration induced fiber swelling and pore remodeling. The scaffold supported APC adhesion, spreading, and metabolic activity over 72 h. Under LPS stimulation, APCs maintained metabolic activity and showed robust mineralization potential after 21 days, comparable to the positive control.</p><p><strong>Conclusion: </strong>The co-electrospun PLGA scaffold showed physicochemical properties compatible with tissue ingrowth and supported APCs function under inflammatory conditions. By providing a controlled microenvironment, it represents a potential scaffold design for future REPs.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"34 ","pages":"e20260524"},"PeriodicalIF":3.0,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13668469/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149038678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hypodontia as an extended phenotype in families with nonsyndromic oral clefts: findings from a 39-family clinical cohort.","authors":"Ana Laura Herrera Farha, Otávio Pagin, Izabel Maria Marchi de Carvalho, Ariadne Letra, Lucimara Teixeira das Neves","doi":"10.1590/1678-7765-2026-0193","DOIUrl":"10.1590/1678-7765-2026-0193","url":null,"abstract":"<p><strong>Introduction: </strong>Hypodontia is frequent in individuals with nonsyndromic oral clefts (NSOC) and has been proposed as a potential extended phenotype reflecting increased genetic susceptibility. However, hypodontia within the cleft area likely results from local anatomical disruption rather than a shared genetic etiology, limiting its usefulness in recurrence-risk assessment. Hypodontia outside the cleft area, though less prevalent, is more likely to reflect a genetically driven disturbance in odontogenesis and a more meaningful marker of familial susceptibility.</p><p><strong>Objective: </strong>In this observational cohort study, we investigated whether parents with NSOC and hypodontia outside the cleft area have a higher rate of cleft recurrence in their offspring compared with parents without hypodontia.</p><p><strong>Methodology: </strong>From a longitudinal cohort of 358 individuals with NSOC followed at the Hospital for Rehabilitation of Craniofacial Anomalies, Bauru, Brazil, from 2020 to 2022, participants who had biological children were interviewed regarding cleft recurrence and family history. Eligibility criteria included confirmed NSOC, at least one biological child, and availability of panoramic radiographs for hypodontia assessment.</p><p><strong>Results: </strong>Individuals were categorized as NSOC with hypodontia outside the cleft area (CH group) or isolated NSOC without hypodontia (IC group). Thirty-nine families met the inclusion criteria (9 CH; 30 IC). Cleft recurrence proportions were 22.2% in the CH group and 13.3% in the IC group. Descriptive and inferential analyses were performed; Fisher's exact test was employed for comparisons. The estimated relative risk was 1.67 (95% confidence interval [CI]: 0.36-7.66; p=0.607). Although this difference was not statistically significant, the observed phenotypic pattern raises the hypothesis that hypodontia outside the cleft region may represent a potential marker of developmental susceptibility and etiological heterogeneity in NSOC.</p><p><strong>Conclusion: </strong>These findings support the value of comprehensive dental phenotyping in craniofacial research and warrant further investigation in larger family-based cohorts integrating phenotypic and genetic approaches.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"34 ","pages":"e20260193"},"PeriodicalIF":3.0,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13668464/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149038667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physicomechanical and biological properties of additively manufactured photosensitive resins for provisional crowns using affordable LCD printer.","authors":"Marina de Brito Teixeira, Marcelo Pereira Brod, Douver Michelon, Adriana Fernandes da Silva, Evandro Piva, Rafael Guerra Lund, Wellington Luiz de Oliveira da Rosa","doi":"10.1590/1678-7765-2026-0157","DOIUrl":"10.1590/1678-7765-2026-0157","url":null,"abstract":"<p><strong>Introduction: </strong>Digital technologies and additive manufacturing are transforming restorative dentistry by enabling the production of highly customized provisional crowns using cost-effective methods.</p><p><strong>Objective: </strong>This study evaluated the physicomechanical and biological properties of photosensitive resins designed for Digital Light Processing (DLP) printers when used to fabricate provisional restorations with an affordable Liquid Crystal Display (LCD) printer.</p><p><strong>Methodology: </strong>Three resins - NextDent C&B (ND), Cosmos Temp (CT), and Prizma BioProv (PB) - were tested and compared against bis-acryl (BR) and self-cure PMMA (SA) controls. Evaluated properties included flexural strength, elastic modulus (immediate, 30, and 90 days), Knoop microhardness, radiopacity, surface roughness, water sorption, solubility, and dimensional accuracy. Biological assessment employed L929 fibroblast cell viability at 1, 7, and 14 days. Data were analyzed using One-way or Two-way ANOVA and Tukey's test (p<0.05).</p><p><strong>Results: </strong>CT exhibited the highest flexural strength, elastic modulus, and Knoop microhardness, alongside the lowest surface roughness, water sorption, and solubility compared to all other materials. ND showed lower surface roughness than controls but higher water sorption. PB presented mechanical properties comparable to those of the controls, but with higher roughness and solubility. All 3D-printed resins exhibited low radiopacity, and, with the exception of BR, cell viability decreased significantly for all materials up to day 14.</p><p><strong>Conclusion: </strong>Selected photosensitive resins processed via an affordable LCD printer showed physicomechanical performance comparable to traditional bis-acryl and PMMA resins in fabricating provisional crowns. However, the low radiopacity observed for all 3D-printed materials and the time-dependent decrease in cell viability represent limitations that warrant further investigation.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"34 ","pages":"e20260157"},"PeriodicalIF":3.0,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149038720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of machine learning techniques to automate speech mispronunciation detection in children and adolescents.","authors":"Nazila Ameli, Mahshid Nik Ravesh, Luan Matheus Trindade Dalmazo, Karen Pollock, Daniel DeSantis, Manuel Lagravere, Hollis Lai","doi":"10.1590/1678-7765-2026-0078","DOIUrl":"10.1590/1678-7765-2026-0078","url":null,"abstract":"<p><strong>Introduction: </strong>Speech sound disorders (SSDs) are common in children and can affect academic and psychosocial outcomes. Clinical identification relies on expert auditory-perceptual assessment, which is time-intensive and may vary across raters. Automated screening tools could support triage when access to specialists is limited.</p><p><strong>Objective: </strong>This study aimed to develop and evaluate a deep learning (DL) classifier for detecting mispronunciation errors in standardized pediatric speech recordings obtained using a structured fricative-focused word elicitation protocol, using expert-adjudicated labels as the reference standard.</p><p><strong>Methodology: </strong>In this cross-sectional study, we analyzed 100 participants (6-18 years) providing 1,800 standardized word recordings. Two expert speech-language pathologists (SLPs) labeled recordings as mispronunciation present vs. absent; disagreements were adjudicated by a third SLP. Inter- and intra-rater reliability were assessed. Audio was denoised and standardized to 16 kHz. A pretrained transformer speech model (WavLM Base+) was fine-tuned for recording-level binary classification. Class imbalance was addressed using augmentation, weighted sampling, and focal loss. Performance was assessed on a held-out test set at the recording level using accuracy, sensitivity, specificity, precision, F1-score, and area under the ROC curve (AUC).</p><p><strong>Results: </strong>The overall recording-level prevalence of speech sound errors was 16.15%, with the \"th\" category (/θ/ + /ð/) being the most frequently mispronounced phoneme group. No significant differences were observed by sex, age group (6-9, 10-13, 14-18 years), or malocclusion status (p > 0.05). Labeling showed strong agreement (Cohen's κ = 0.84; intra-rater reliability 0.89-0.92). On the test set (253 recordings), the model achieved 90.9% accuracy, 77.8% sensitivity, 98.2% specificity, 95.9% precision, and AUC = 0.936.</p><p><strong>Conclusions: </strong>Fine-tuned pretrained speech representations demonstrated promising performance for screening pediatric mispronunciation errors under standardized recording conditions using expert labels. The high-specificity profile supports its use as a screening and triage decision-support tool, while future studies are needed to validate performance across broader clinical and real-world settings.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"34 ","pages":"e20260078"},"PeriodicalIF":3.0,"publicationDate":"2026-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13668468/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148919916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The modulatory effect of oxygen -releasing gel versus 0.8% hyaluronic acid gel incorporated hydroxyapatite bone graft in the management of grade II mandibular furcation defects: a randomized clinical control trial.","authors":"Pavitra Jayakumar, Sharmistha Vijay, Debopriya Chatterjee, Setu Mathur","doi":"10.1590/1678-7765-2026-0115","DOIUrl":"10.1590/1678-7765-2026-0115","url":null,"abstract":"<p><strong>Aim: </strong>The intricate architecture of multirooted teeth challenges access to the furcation for treatment. This study aimed to assess the clinical and radiographic efficacy of oxygen-releasing oral gel and 0.8% hyaluronic acid gel along with hydroxyapatite bone graft to treat Class II furcation defects.</p><p><strong>Methodology: </strong>Overall, 45 (one site per participant, aged from 20 to 60 years) mandibular molars with periodontitis (stage II and stage III, grade A and B) and class II buccal furcation defects were treated in this study. Selected sites were randomly divided into three groups. In group 1, placebo gel was placed after open flap debridement (OFD), in group 2- 0.8% hyaluronic acid gel + hydroxyapatite bone graft was placed after OFD, and, in group 3, oxygen-releasing oral gel + hydroxyapatite bone graft was placed after furcation defect OFD. Clinical parameters such as gingival index, plaque index, relative vertical clinical attachment level, relative horizontal clinical attachment level, and probing pocket depth were recorded at baseline and six months later. Radiographic measurements-intra bony defect & % bone fill-were measured and calculated by cone beam computed tomography. Results: The mean reduction in intra bony defect was highest in Group 3 (1.433±0.594), followed by Groups 2 (1.000 ± 0.000) and 1 (0.700±0.414). Group 1 showed the lowest mean bone fill (34.69%±12.14), which was significantly lower than in Groups 2 (46.87%±4.71) and 3 (50.93%±4.62), with p-values of (p<0.001) in both comparisons.</p><p><strong>Conclusion: </strong>Within the limitations of this study, its results imply that oxygen-releasing gel along with hydroxyapatite bone graft in surgical periodontal therapy to treat class II furcation defects has shown superior regenerative potential. Clinical Trial Register: CTRI/2025/02/081311.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"34 ","pages":"e20260115"},"PeriodicalIF":3.0,"publicationDate":"2026-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13668470/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148886897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potency of moderate-intensity exercise on caspase-8 and caspase-9 expression in a DMBA-induced oral epithelial dysplasia rat model.","authors":"Anis Irmawati, Rasha Fatimah Abdullah, Athallah Reyhan Darmawan, Retno Indrawati Roestamadji, Ira Arundina, Noor Faizah Balqis, Fiki Muhammad Ridho, Noeroel Widajati, Raed Labib","doi":"10.1590/1678-7765-2026-0438","DOIUrl":"https://doi.org/10.1590/1678-7765-2026-0438","url":null,"abstract":"<p><strong>Background: </strong>Oral squamous cell carcinoma (OSCC) accounts for approximately 90% of oral cancers, with tobacco exposure representing one of its major risk factors. The tobacco carcinogen 7,12-dimethylbenz(a)anthracene (DMBA) induces oral epithelial dysplasia, a premalignant lesion. Caspase-8 and caspase-9 are initiator caspases that trigger apoptosis in dysplastic cells. Currently, disease management focuses on prevention. Moderate-intensity exercise has been reported to modulate apoptosis-related signaling and may contribute to cancer prevention.</p><p><strong>Objective: </strong>To evaluate the potency of moderate-intensity exercise on caspase-8 and -9 expression in a DMBA-induced oral dysplasia rat model.</p><p><strong>Methodology: </strong>A total of 24 Wistar rats were randomly divided into four groups: K1 (no exercise, no DMBA), K2 (exercise, no DMBA), K3 (no exercise, DMBA), K4 (exercise, DMBA). Exercise consisted of moderate-intensity swimming at 70% MWC, performed 3x/week for 6 weeks. DMBA (0.02 mg/kg body weight) was injected into the buccal mucosa of the canine region twice weekly for 2 weeks. At the 7th week, the rats were anesthetized with ketamine (10 mg/kg body weight), euthanized by cervical dislocation, and buccal tissue specimens were collected. Immunohistochemical staining was performed to obtain caspase-8 and -9 expression. Data were analyzed in SPSS version 29.0.</p><p><strong>Results: </strong>All groups presented significant differences in caspase-8 (p = 0.004) and caspase-9 (p <0.001) expression. Caspase-8 and caspase-9 expression was significantly higher in K4 than in K3, with expression restored to levels comparable to the untreated control group (K1).</p><p><strong>Conclusion: </strong>Moderate-intensity exercise restored caspase-8 and caspase-9 expression suppressed by DMBA exposure, suggesting preservation of apoptotic signaling in DMBA-induced Wistar rats.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"34 ","pages":"e20260438"},"PeriodicalIF":3.0,"publicationDate":"2026-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148840415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Risk Classification Instrument for Orofacial Myofunctional Disorders - ICR/DMO: Reliability (Part III).","authors":"Herick Santos Assis, Íkaro Daniel de Carvalho Barreto, Giédre Berretin-Felix, Andréa Monteiro Correia Medeiros","doi":"10.1590/1678-7765-2026-0314","DOIUrl":"10.1590/1678-7765-2026-0314","url":null,"abstract":"<p><strong>Objective: </strong>To assess the reliability of the Risk Classification Instrument for Orofacial Myofunctional Disorders (ICR/DMO).</p><p><strong>Methodology: </strong>This reliability study was conducted as part of the broader validation process of the ICR/DMO. Ninety caregivers of infants and preschoolers participated and were allocated into three age groups: G1=30 infants aged 1-5 months; G2=30 infants aged 6-23 months; and G3=30 preschoolers aged 24-71 months. Participants were recruited from two public daycare centers and a university pediatric clinic. The ICR/DMO was administered in person by a single speech-language pathologist (A1) by means of individual interviews with the caregivers, with responses registered in real time and audio-recorded. The audio recordings were analyzed by three additional raters (A2a, A2b, and A2c). After calibration, each rater received 30 audio recordings according to the following group allocation: A2a (G1), A2b (G2), and A2c (G3). Inter-rater agreement (100% of the sample; n=90) and intra-rater reproducibility (20% of the sample; n=18) were assessed using Kendall's tau (Kendall's τ). The following interpretation was adopted: weak (<0.4), moderate (0.4-0.7), and strong (>0.7), with p<0.05.</p><p><strong>Results: </strong>Inter- and intra-rater agreement were observed both for the total scores and for individual instrument items. For the total scores, inter-rater agreement was moderate in G1 (τ=0.576; p=0.001); strong in G2 (τ=0.775; p<0.001), and moderate in G3 (τ=0.583; p<0.001). In all groups, the retest showed strong agreement (G1: τ=0.786; p=0.032; G2: τ=0.881; p=0.002; G3: τ=0.926; p=0.014). For the individual items, both inter-rater and intra-rater agreement showed satisfactory rates of observed agreement.</p><p><strong>Conclusion: </strong>The ICR/DMO demonstrated evidence of reliability across the three age groups, with inter-rater agreement ranging from moderate to good and intra-rater agreement ranging from good to excellent.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"34 ","pages":"e20260314"},"PeriodicalIF":3.0,"publicationDate":"2026-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13668466/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148840504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of the effects of two different wound dressing materials on wound healing in mucosal defects created in diabetic and healthy rats.","authors":"Merve Yalçın, Vildan Bostancı, Özhan Karataş","doi":"10.1590/1678-7765-2026-0096","DOIUrl":"https://doi.org/10.1590/1678-7765-2026-0096","url":null,"abstract":"<p><strong>Introduction: </strong>Diabetes mellitus impairs oral mucosal wound healing by prolonging inflammation and disrupting angiogenesis and growth factor regulation.</p><p><strong>Aim: </strong>To evaluate the effects of Ora-Aid (TBM Corporation, Gwangju, Republic of Korea) and cyanoacrylate (GluStitch, City of Industry, CA, USA) on palatal wound healing in diabetic and healthy rats.</p><p><strong>Methodology: </strong>A total of 146 male Wistar albino rats were used: 144 were divided into six groups: control (C), cyanoacrylate (CY), Ora-Aid (OA), diabetes (D), diabetes+cyanoacrylate (DCY), and diabetes+Ora-Aid (DOA) (n=24 each; 8 per time point), and 2 served as baseline controls. Diabetes was induced by intraperitoneal streptozotocin (STZ; Santa Cruz Biotechnology, Texas, USA) at 60 mg/kg. A 4-mm palatal excisional wound was created. Wound area was measured from photographs using ImageJ v1.54g (Wayne Rasband, National Institutes of Health, USA). Immunohistochemical analyses assessed vascular endothelial growth factor (VEGF), fibroblast growth factor (FGF), interleukin-10, and tumor necrosis factor-alpha. Data were analyzed using the Kruskal-Wallis test and Bonferroni-adjusted Mann-Whitney U tests. Statistical significance was evaluated based on adjusted p-values, with p<0.05 considered significant.</p><p><strong>Results: </strong>In healthy rats, wound areas after cyanoacrylate and Ora-Aid were similar to controls. In diabetic rats, both wound dressings reduced wound area, particularly on day 14 [median (IQR): D, 0.33 (0.82); DCY, 0.27 (0.71); DOA, 0.09 (0.09); D vs DCY, p=0.037; D vs DOA, p=0.002]. Immunohistochemical analyses showed that Ora-Aid more pronouncedly affected VEGF expression in healthy groups and FGF expression in diabetic groups, whereas cyanoacrylate showed time-dependent effects on growth factors and inflammatory markers.</p><p><strong>Conclusion: </strong>Ora-Aid and cyanoacrylate positively influenced oral mucosal wound healing under diabetic conditions. Ora-Aid more prominently supported the VEGF-related angiogenic response under healthy conditions and FGF-related fibroblastic activity under diabetic conditions, whereas cyanoacrylate showed time-dependent regulatory effects on growth factors and the inflammatory response.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"34 ","pages":"e20260096"},"PeriodicalIF":3.0,"publicationDate":"2026-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148793611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"FOXO3a promotes odontoblast differentiation in human dental pulp cells in coordination with RUNX2.","authors":"Su-Kyung Son, Jung-Sun Moon, Na-Ri Jung, Jee-Hae Kang, Bin-Na Lee, Sun-Hun Kim, Min-Seok Kim","doi":"10.1590/1678-7765-2026-0114","DOIUrl":"https://doi.org/10.1590/1678-7765-2026-0114","url":null,"abstract":"<p><strong>Introduction: </strong>Forkhead box O3a (FOXO3a) is a stress-responsive transcription factor involved in cellular differentiation across multiple tissues. However, its role in odontoblast differentiation and dentin matrix formation remains poorly defined.</p><p><strong>Objective: </strong>This study investigated the functional involvement of FOXO3a in odontoblast differentiation and its association with key odontogenic regulators.</p><p><strong>Methodology: </strong>Human dental pulp cells (HDPCs) were induced to undergo odontoblast differentiation in vitro. FOXO3a gene and protein expression patterns were examined during differentiation and in postnatal Sprague Dawley rat molars. Gain- and loss-of-function approaches assessed the role of FOXO3a in odontogenic differentiation. Odontoblast marker expression, alkaline phosphatase activity, and mineralized matrix formation were evaluated. RUNX2 transcriptional activity was analyzed using promoter reporter assays, and the interactions between FOXO3a and AKT signaling were examined through pharmacological modulation.</p><p><strong>Results: </strong>FOXO3a expression increased during early odontoblast differentiation. FOXO3a silencing reduced odontogenic marker expression, including DSPP, DMP1, and RUNX2, and significantly attenuated mineralization, whereas FOXO3a overexpression enhanced these effects. FOXO3a increased RUNX2 transcriptional activity and cooperated with RUNX2 to regulate DSPP promoter activity. AKT activation partially rescued odontogenic marker expression in FOXO3a-silenced cells, supporting functional interplay between these pathways.</p><p><strong>Conclusions: </strong>FOXO3a contributes to odontoblast differentiation by coordinating with RUNX2-associated transcriptional activity and interacting dynamically with AKT signaling. FOXO3a may represent a regulatory component of dentin matrix gene expression, relevant to regenerative endodontic applications.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"34 ","pages":"e20260114"},"PeriodicalIF":3.0,"publicationDate":"2026-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148793571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}