Journal of Applied Oral Science最新文献

{"title":"Synthetic sticky bone grafts enhance bone regeneration: a preclinical evaluation in rat models.","authors":"Lei Li, Haojie Lin, Siyu Jin, Shuchang Hu, Wei Sun, Wei Ji","doi":"10.1590/1678-7757-2025-0108","DOIUrl":"https://doi.org/10.1590/1678-7757-2025-0108","url":null,"abstract":"<p><strong>Objectives: </strong>Deproteinized bovine bone minerals (DBBMs) are effective for bone regeneration. However, their limited plasticity can hinder extensive bone defects treatment. This study aimed to develop a composite bone grafting material that is easy to deploy surgically and promotes robust bone regeneration.</p><p><strong>Methodology: </strong>DBBM particles were mixed with a clinical-grade gelatin-based hemostatic gel (w/v ratio of 2/3) to create a composite material referred to as synthetic sticky bone (SSB). Structural properties were assessed using confocal laser scanning microscopy and scanning electron microscopy. To evaluate bone regenerative capacity, 20 male Sprague Dawley rats (eight to ten weeks old) with critical-size jawbone defects were treated with SSB, DBBM, or gelatin gel alone, with an empty defect as a control. Samples were collected at two and four weeks for microcomputed tomography (μCT) analysis of bone volume/total tissue volume (BV/TV), trabecular thickness (Tb. Th), trabecular number (Tb. N), and trabecular separation (Tb. Sp). Histological analyses were conducted to examine material remnants and bone formation.</p><p><strong>Results: </strong>SSB showed a binary paste-like composite property with enhanced injectability and plasticity. μCT and histological assessments confirmed that the SSB-treated group had significantly greater new bone formation compared to the DBBM-treated group after four weeks.</p><p><strong>Conclusions: </strong>SSB, which is a paste-like composite of DBBM particles, and a clinical-grade gelatin-based hemostatic gel demonstrated improved structural plasticity and enhanced bone regeneration, offering a promising solution for treating extensive irregular bone defects.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"33 ","pages":"e20250108"},"PeriodicalIF":2.2,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144325800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic effect of Maquiberry cystatin, sodium fluoride and stannous chloride for the prevention of initial dental erosion in vitro.","authors":"Vinícius Taioqui Pelá, Thelma Lopes Silva, Even Akemi Taira, Geórgia Almeida Sant'ana, Letícia Oba Sakae, Flávia Mauad Levy, Taís Scaramucci Forlin, Eduardo Pereira de Souza, Talita Mendes Oliveira Ventura, Thiago Saads Carvalho, Adrian Lussi, Marília Afonso Rabelo Buzalaf","doi":"10.1590/1678-7757-2024-0479","DOIUrl":"https://doi.org/10.1590/1678-7757-2024-0479","url":null,"abstract":"<p><strong>Objective: </strong>This study analyzed the synergistic effect between a recombinant Maquiberry (MaquiCPI-3) protein, sodium fluoride (NaF), and stannous chloride (SnCl2) against initial dental erosion in vitro.</p><p><strong>Methodology: </strong>A total of 98 bovine enamel samples were prepared and allocated to seven treatment groups (n=14/group) as follows: deionized water (Water); commercial solution, 800 ppm Sn+2, 500 ppm F-, ElmexTM, Erosion, GABA, Therwil, BL, CH (Elmex); 500 ppm of fluoride (F-) from NaF (NaF); 500 ppm of F- from NaF and 800 ppm of tin (Sn+2) from SnCl2 (NaF+SnCl); 0.5 mg/mL MaquiCPI-3 (MaquiCPI-3); combination of MaquiCPI-3 and NaF (Maqui+NaF) and; combination of MaquiCPI-3, NaF and SnCl (Maqui+NaF+SnCl). Samples were treated with the respective solutions (250 μl, 2 min, 37°C, 250 rpm). After forming the acquired enamel pellicle (AEP) by adding human saliva from nine volunteers (250 μl, 1 h, 37°C, 250 rpm), the samples underwent acid challenge (1 mL, 1% citric acid, pH 3.6, 1 min, 25°C, 250 rpm). All procedures were performed in triplicate. Surface microhardness change percentage (%SMC) and relative surface reflection intensity (%SRI) were measured and analyzed by One-way ANOVA/Tukey's tests (p<0.05).</p><p><strong>Results: </strong>The Elmex, NaF, NaF+SnCl, MaquiCPI-3, Maqui+NaF, and Maqui+NaF+SnCl groups showed significantly lower %SMC compared with Water. The NaF, Sn+2, NaF+SnCl, MaquiCPI-3, Maqui+NaF and Maqui+NaF+SnCl groups exhibited significantly greater protection compared with Elmex. Maqui+NaF+SnCl revealed better enamel protection (significant) when compared with the MaquiCPI-3 and Maqui+NaF groups. Elmex, NaF+SnCl, MaquiCPI-3, Maqui+NaF, and Maqui+NaF+SnCl had a significantly higher %SRI compared to the Water and NaF groups, which did not differ between each other.</p><p><strong>Conclusion: </strong>All treatment solutions provided protection against initial dental erosion in vitro. Formulations containing Maqui+NaF+SnCl2 offered superior enamel protection compared with MaquiCPI-3 alone.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"33 ","pages":"e20240479"},"PeriodicalIF":2.2,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EZH2 expression is restricted to malignant salivary gland tumors.","authors":"Dusit Bumalee, Prow Janjarussakul, Jintana Pankam, Tawepong Arayapisit, Nakarin Kitkumthorn, Teerat Chanamorn, Amanda Li Yin Goh, Puangwan Lapthanasupkul","doi":"10.1590/1678-7757-2025-0123","DOIUrl":"https://doi.org/10.1590/1678-7757-2025-0123","url":null,"abstract":"<p><strong>Background: </strong>Enhancer of zeste homolog 2 (EZH2) configures a histone methyl transferase enzyme that mediates the epigenetic silencing of target genes. Its overexpression has been related to tumor proliferation, metastasis, and poor prognoses. However, the study of this protein in salivary gland tumors (SGTs) remains limited.</p><p><strong>Objective: </strong>This study aimed to investigate EZH2 expression in common intra-oral SGTs, including pleomorphic adenoma (PA), mucoepidermoid carcinoma (MEC), and adenoid cystic carcinoma (ACC).</p><p><strong>Methodology: </strong>A total of 48 formalin-fixed paraffin-embedded tissue samples were analyzed, including 17 PA, 21 MEC, and 10 ACC cases. Immunohistochemical staining was performed using a rabbit recombinant monoclonal EZH2 antibody. EZH2 expression was described and quantified by the percentage of stained tumor cells.</p><p><strong>Results: </strong>Nuclear EZH2 expression occurred in most malignant SGTs (90.00% of ACC and 80.95% of MEC cases), whereas only one benign case of PA (5.88%) showed positive mild staining. Statistical analysis showed a significant difference in the mean of percentage positive cells in SGTs (p<0.001), particularly between benign and malignant SGTs (p<0.001). However, this study observed no significant differences between MEC and ACC (p=0.457).</p><p><strong>Conclusion: </strong>EZH2 expression significantly differed between malignant and benign SGTs, supporting its potential as a diagnostic marker for malignancy in these tumors.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"33 ","pages":"e20250123"},"PeriodicalIF":2.2,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of obstructive sleep apnea risk in different types of cleft lip and palate.","authors":"Patrícia Jost, Gabriela Utrago, Felícia Miranda, Rita de Cássia Moura Carvalho Lauris, João Poiani, Daniela Garib","doi":"10.1590/1678-7757-2024-0515","DOIUrl":"https://doi.org/10.1590/1678-7757-2024-0515","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to assess the risk of obstructive sleep apnea (OSA) in different types of cleft using the Pediatric Sleep Questionnaire (PSQ). The influence of sex, age, body mass index (BMI), and history of adenotonsillectomy surgery were also evaluated.</p><p><strong>Methodology: </strong>Parents or legal guardians of 195 patients with nonsyndromic cleft lip, lip and palate, and isolated palate aged from 5 to 18 years (9.11±3.09 years) were invited to answer the PSQ. The sample included 94 individuals with unilateral or bilateral complete cleft lip and palate, 56 individuals with cleft lip, and 45 individuals with isolated cleft palate. The frequency of high and low risk was estimated for the complete sample. Multiple logistic regression analysis was used to assess the association between sex, age, cleft type, adenotonsillectomy surgery, and BMI with the occurrence of high-risk of OSA. Significance was considered at 5%.</p><p><strong>Results: </strong>A high risk of OSA was observed in 51.3% of the sample. The high risk of OSA was present in 51.8%, 53.1%, and 46.6% of subjects with cleft lip, cleft lip and palate, and isolated cleft palate, respectively. History of adenotonsillectomy surgery was a predictor of high risk of OSA (OR=6.94). The other variables were not associated with OSA high risk.</p><p><strong>Conclusion: </strong>Different cleft types had similar frequency of OSA high risk. Patients with history of adenotonsillectomy surgery still presented high risk of OSA.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"33 ","pages":"e20240515"},"PeriodicalIF":2.2,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144274997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of ibuprofen on amelogenesis in Wistar rats.","authors":"Sharon Oyhanart, Carlos A Barcenas, Ana Maria Collet, Andrea Edith Kaplan, Patricia Monica Mandalunis","doi":"10.1590/1678-7757-2024-0300","DOIUrl":"https://doi.org/10.1590/1678-7757-2024-0300","url":null,"abstract":"<p><strong>Background: </strong>This work aimed to evaluate the effect of ibuprofen on amelogenesis using a model of continuously growing incisors in Wistar rats.</p><p><strong>Methodology: </strong>A total of eight female Wistar rats at weaning age were assigned to one of two groups (IBU and control). They were administered an 80 mg/kg dose of ibuprofen or an equivalent volume of distilled water for three weeks, then euthanized on day 16 of the experiment. Right hemimandibles were used to assess the mineral density of enamel using microtomography. The left hemimandibles were decalcified and processed to obtain sections, stained with Hematoxylin Eosin or immunohistochemical detection of amelogenin. Based on photomicrographs of hemimandibles, ameloblast and papillary layer height of the enamel and enamel organic matrix width were determined. The percentage of positive amelogenin was determined in immunohistochemically processed sections. Results were analyzed using Student's t test.</p><p><strong>Results: </strong>IBU-treated animals showed lower body weight gain throughout the experiment (p<0.05). Mineral density and enamel thickness showed no significant differences. No significant differences in the height of the papillary layer or the width of the organic matrix were observed. Amelogenin expression in the ameloblast layer was lower in the experimental group. No significant difference was found between groups.</p><p><strong>Conclusion: </strong>The results obtained in this study model suggest that ibuprofen itself might not alter the amelogenesis process.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"33 ","pages":"e20240300"},"PeriodicalIF":2.2,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of manufacturing methods and use of CoCr-based abutments on vertical and horizontal marginal fit and torque loss in implant-supported prostheses.","authors":"João Pedro Justino DE Oliveira Limirio, Jéssica Marcela de Luna Gomes, Joel Ferreira Santiago-Junior, Cleidiel Aparecido Araújo Lemos, Maria Cristina Rosifini Alves Rezende, Eduardo Piza Pellizzer","doi":"10.1590/1678-7757-2024-0589","DOIUrl":"https://doi.org/10.1590/1678-7757-2024-0589","url":null,"abstract":"<p><strong>Objectives: </strong>This in vitro study evaluated implant-supported single crowns with external connections fabricated using different techniques (cast and CAD/CAM) and materials (metal-ceramic and translucent zirconia) regarding vertical and horizontal marginal fit and torque loss before and after mechanical cycling.</p><p><strong>Methodology: </strong>A total of 50 specimens prepared using cast (lost wax) and CAD/CAM were divided into five groups-MC (metal-ceramic crowns), ZrB (Co-Cr base, coping milled in zirconia and feldspathic ceramic veneering), Zr (coping milled in zirconia and feldspathic ceramic veneering), MZrB (Co-Cr base and monolithic translucent zirconia crown), and MZr (monolithic translucent zirconia crown)-and subjected to 5×10⁶ mechanical cycles at a 30° angle at 37°C, and an applied load of 150N at 2Hz. All crowns were screwed with a 30Ncm torque. Vertical and horizontal marginal misfit (measured using a 3D optical microscope) and torque loss were assessed before and after mechanical cycling. Statistical analyses used a significance value of 0.05.</p><p><strong>Results: </strong>Before cycling, MC (93.93±22.84µm) and MZrB (66.12±11.87µm) (p<0.05) crowns showed significantly higher vertical marginal misfit values than Zr (49.92±3µm) and MZr (49.76±3,9µm). ZrB (59.96±4.66µm) crowns exhibited no statistically significant difference when compared with the other groups. MC crowns presented the highest horizontal marginal misfit values before and after cycling in group comparisons (p<0.05). MZrB had the highest torque loss (10N), with a statically significant difference when compared with MZr and Zr (p<0.05). Comparison between all groups showed no significant difference regarding the effect of mechanical cycling (p>0.05).</p><p><strong>Conclusion: </strong>CAD/CAM manufacturing resulted in lower vertical and horizontal marginal misfit values, especially for monolithic translucent zirconia crowns (MZr) before and after mechanical cycling. All groups presented torque loss before and after mechanical cycling.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"33 ","pages":"e20240589"},"PeriodicalIF":2.2,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144225584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comparative study of three liquid platelet concentrates on human primary osteoblast activity: an in vitro study.","authors":"Vichuda Chattrathikul, Putida Pinthonglor, Chayarop Supanchart, Supatra Sangin","doi":"10.1590/1678-7757-2024-0575","DOIUrl":"10.1590/1678-7757-2024-0575","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the effects of concentrated platelet-rich fibrin (C-PRF), injectable platelet-rich fibrin (i-PRF), and platelet-rich plasma (PRP) on cellular activity of human primary osteoblasts.</p><p><strong>Methodology: </strong>C-PRF, i-PRF, and PRP were prepared from five donors and pre-cultured in 5 mL of culture medium for three days. Human primary osteoblasts were seeded and cultured with 20% conditioned medium derived from the three platelet concentrates. Then, osteoblast viability was assessed at 24 h; proliferation at one, three, and five days; differentiation at seven days; mineralization at 14 days; and gene expression RUNX family transcription factor 2 (RUNX2), alkaline phosphatase, biomineralization associated (ALPL), collagen type I alpha 1 chain (COL1A1), and osteocalcin (OCN) at three and 14 days were investigated.</p><p><strong>Results: </strong>Osteoblasts cultured with C-PRF, i-PRF, and PRP demonstrated excellent biocompatibility. Proliferation was significantly higher in all platelet concentrates compared to the controls at one, three, and five days, with no significant differences among them, except on day one. Alkaline phosphatase and Alizarin Red S staining were significantly higher in the C-PRF and i-PRF groups compared to the PRP and control groups. However, RUNX2, ALPL, COL1A1, and OCN mRNA levels did not differ significantly among the three platelet concentrates throughout the study period.</p><p><strong>Conclusion: </strong>Our study indicates that the three liquid platelet concentrates enhance human osteoblast activity. C-PRF and i-PRF promoted greater differentiation and mineralization than PRP. These findings show that all liquid platelet concentrates positively influence human osteoblast proliferation and differentiation, making them suitable for clinical applications requiring bone regeneration.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"33 ","pages":"e20240575"},"PeriodicalIF":2.2,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12176320/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144225582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of TiF4, CPP-ACP, and NaF in preventing demineralization in irradiated bovine enamel and dentin in vitro.","authors":"Lin Yao, Yanyao Li, Di Fu, Mengzhen Ji, Ling Zou, Li Jiang","doi":"10.1590/1678-7757-2024-0524","DOIUrl":"https://doi.org/10.1590/1678-7757-2024-0524","url":null,"abstract":"<p><strong>Objectives: </strong>To investigate the effects of sodium fluoride (NaF), casein phosphopeptide-amorphous calcium phosphate (CPP-ACP), and titanium tetrafluoride (TiF4) on the prevention of demineralization in irradiated bovine enamel and dentin in vitro.</p><p><strong>Methodology: </strong>The enamel and dentin sample blocks were subjected to 50 Gy of radiation and divided into five groups (n=13): the deionized distilled water group, the NaF group, the CPP-ACP group, the NaF + CPP-ACP group, and the TiF4 group. After being treated with various materials for 30 minutes, the samples were remineralized for 12 hours and demineralized for 48 hours. The samples were then evaluated by scanning electron microscope (SEM), atomic force microscope (AFM), energy dispersive spectrometer (EDS), and transverse microradiography (TMR). Data were analyzed by ANOVA and the Kruskal-Wallis H test (α=0.05).</p><p><strong>Results: </strong>SEM and TMR indicated that the TiF4 group promoted more mineral deposits on the enamel and dentin samples, showing the least mineral loss and the lowest lesion depth. AFM results showed that the NaF + CPP-ACP group had the lowest enamel roughness (p<0.05), whereas the TiF4 group showed the lowest roughness in dentin samples (p<0.05). EDS showed that titanium (Ti) was deposited on the surface of the TiF4 group samples, whereas the NaF + CPP-ACP group more greatly aggregated fluorine.</p><p><strong>Conclusion: </strong>TiF4 significantly impacted the prevention of demineralization in irradiated dental hard tissues. Combining NaF and CPP-ACP more effectively prevented demineralization than either agent used alone.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"33 ","pages":"e20240524"},"PeriodicalIF":2.2,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144225583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Generalized gingivitis-related salivary proteomic profile in pregnant women with obesity: insights into biological mechanisms assessed by Tandem Mass Spectrometry.","authors":"Laura Teodoro de Marchi, Amanda Borges Pirondi, Talita Mendes Oliveira Ventura, Larissa Tercilia Grizzo, Marília Afonso Rabelo Buzalaf, Gerson Aparecido Foratori-Junior","doi":"10.1590/1678-7757-2025-0031","DOIUrl":"10.1590/1678-7757-2025-0031","url":null,"abstract":"<p><strong>Aim: </strong>This cross-sectional study investigated the salivary proteomic profile associated with generalized gingivitis in pregnant women with obesity.</p><p><strong>Methodology: </strong>Pregnant women in the third trimester (≥27 weeks of gestation) were divided into two groups based on bleeding on probing (BOP): G1 (BOP>50%; n=9) and G2 (BOP 0-30%; n=9). Collected unstimulated saliva samples were individually analyzed using nano liquid chromatography electron spray ionization tandem mass spectrometry. Identified proteins were classified according to gene ontology for biological processes, molecular functions, immune system involvement, and cellular components. Differential protein expression was determined using thresholds of p<0.05 for downregulation and 1-p>0.95 for up-regulation proteins.</p><p><strong>Results: </strong>Of the 183 identified proteins, 100 were shared between groups, totaling 57 up-regulated and 27 downregulated proteins in G1. Key biological processes included antimicrobial humoral response and hydrogen peroxide catabolism, with proteins linked to immune function and endopeptidase regulation. Functional analysis showed that Lactotransferrin (5-fold increase in G1), Haptoglobin (4-fold), and Immunoglobulin J chain (3-fold) were up-regulated, whereas Statherin (5-fold) and Protein S100-A8 (4-fold) were downregulated in G1.</p><p><strong>Conclusions: </strong>Pregnant women with obesity and generalized gingivitis exhibited a distinct salivary proteomic profile characterized by the up-regulation of immune-related proteins and downregulation of tissue-protective proteins. These findings suggest potential salivary biomarkers for detection and targeted management of periodontal inflammation in this high-risk population.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"33 ","pages":"e20250031"},"PeriodicalIF":2.2,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12176321/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144078265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nasopalatine duct cyst: a multicenter retrospective study of 63 cases and an integrative review of the clinical, imaginological and histopathological aspects.","authors":"Diana Estefania Ramos Peña, Mariana de Sá Alves, Samuel Porfírio Xavier, Tiago Novaes Pinheiro, Paulo Sérgio da Silva Santos, Izabel Regina Fischer Rubira-Bullen, Ana María Cadavid Giraldo, Pilar Schmitt Sanjuan Navarro, Leonor Victoria González-Pérez, Cintia Micaela Chamorro Petranacci, Mario Perez-Sayáns, Ana Lia Anbinder, Janete Dias Almeida, Ana Carolina Fragoso Motta","doi":"10.1590/1678-7757-2024-0539","DOIUrl":"10.1590/1678-7757-2024-0539","url":null,"abstract":"<p><strong>Introduction: </strong>The nasopalatine duct cyst (NPDC) is the most common nonodontogenic jaw cyst, with a higher prevalence reported in males. Diagnosing jaw cysts remains challenging for general dentists due to their overlapping clinical and radiographic presentations. An updated case series and integrative literature review may assist in improving the accurate diagnosis of NPDC.</p><p><strong>Objective: </strong>This study aimed to describe the clinicopathological and imaging characteristics of 63 NPDC cases and to review previously reported cases in the literature.</p><p><strong>Methodology: </strong>An international, multicenter, retrospective NPDC case series was conducted. Demographic, radiographic, and histopathological data were collected from clinical records. Additionally, a PubMed/MEDLINE search was performed to identify articles on NPDC.</p><p><strong>Results: </strong>A total of 63 NPDC cases were evaluated, with a mean patient age of 47 years and no significant sex predilection. Twenty-one cases were asymptomatic, while 34 presented with symptoms such as pain and swelling. Radiographically, NPDC appeared as a well-defined radiolucent lesion located between the upper central incisors, bordered by a radiopaque margin. The integrative literature review identified 67 studies, comprising 51 case reports, 12 retrospective studies, and four case series, totaling 1,003 reported NPDC cases. The clinicopathological and radiographic findings from the literature aligned with those in this case series.</p><p><strong>Conclusion: </strong>The 63 cases analyzed in this study showed consistent findings across six international centers, with no sex predilection observed, contrasting with the male dominance reported in the literature. NPDC should be considered in the differential diagnosis of intraosseous lesions in the anterior maxilla. Accurate diagnosis requires a combination of radiographic and histopathological evaluations to prevent misdiagnosis and improper treatment.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"33 ","pages":"e20240539"},"PeriodicalIF":2.2,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12061452/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144019608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}