Journal of Applied Oral Science最新文献

{"title":"Factors influencing the response to periodontal therapy in patients with diabetes: post hoc analysis of a randomized clinical trial using machine learning.","authors":"Nidia Castro Dos Santos, Arthur Mangussi, Tiago Ribeiro, Rafael Nascimento de Brito Silva, Mauro Pedrine Santamaria, Magda Feres, Thomas VAN Dyke, Ana Carolina Lorena","doi":"10.1590/1678-7757-2025-0211","DOIUrl":"10.1590/1678-7757-2025-0211","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate factors influencing the response to periodontal therapy in patients with periodontitis and type 2 diabetes mellitus (DM) using machine learning (ML) techniques, considering periodontal parameters, metabolic status, and demographic characteristics.</p><p><strong>Methodology: </strong>We applied machine learning techniques to perform a post hoc analysis of data collected at baseline and a 6-month follow-up from a randomized clinical trial (RCT). A leave-one-out cross-validation strategy was used for model training and evaluation. We tested seven different algorithms: K-Nearest Neighbors, Decision Tree, Support Vector Machine, Random Forest, Extreme Gradient Boosting, and Logistic Regression. Model performance was assessed using accuracy, specificity, recall, and the area under the Receiver Operating Characteristic (ROC) curve (AUC).</p><p><strong>Results: </strong>a total of 75 patients were included. Using the first exploratory data analysis, we observed three clusters of patients who achieved the clinical endpoint related to HbA1c values. HbA1c ≤ 9.4% was correlated with lower PD (r=0.2), CAL (r=0.1), and the number of sites with PD ≥5 mm (r=0.1) at baseline. This study induced AI classification models with different biases. The model with the best fit was Random Forest with a 0.83 AUC. The Random Forest AI model has an accuracy of 80%, a sensitivity of 64%, and a specificity of 87%. Our findings demonstrate that PD and CAL were the most important variables contributing to the predictive performance of the Random Forest model.</p><p><strong>Conclusion: </strong>The combination of nine baseline periodontal, metabolic, and demographic factors from patients with periodontitis and type 2 DM may indicate the response to periodontal therapy. Lower levels of full mouth PD, CAL, plaque index, and HbA1c at baseline increased the chances of achieving the endpoint for treatment at 6-month follow-up. However, all nine features included in the model should be considered for treatment outcome predictability. Clinicians may consider the characterization of periodontal therapy response to implement personalized care and treatment decision-making. Clinical trial registration ID: NCT02800252.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"33 ","pages":"e20250211"},"PeriodicalIF":2.6,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144742179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between multiple genetic polymorphisms and molar-incisor hypomineralization: a population-based study.","authors":"Luíse Gomes-Souza, Aluhê Lopes Fatturi, Rafaela Scariot, Cleber Machado-Souza, Erika Calvano Küchler, João Armando Brancher, Juliana Feltrin-Souza","doi":"10.1590/1678-7757-2025-0074","DOIUrl":"https://doi.org/10.1590/1678-7757-2025-0074","url":null,"abstract":"<p><strong>Background: </strong>Certain genes present variants associated with molar-incisor hypomineralization (MIH) pathogenesis, especially genes encoding enamel development proteins related to morphogenesis, immune response, and hormone transcription and reception, demonstrating that MIH is likely a gene-environment issue with multiple genes having small individual effects.</p><p><strong>Objective: </strong>To evaluate the association between single nucleotide polymorphisms (SNPs) and MIH.</p><p><strong>Methodology: </strong>A sample of 90 children with MIH and 262 children without MIH were included in this study. Calibrated examiners diagnosed MIH (Kappa≥0.75) using the European Academy of Paediatric Dentistry (EAPD) criteria and modified DDE index in clinical exams. SNPs in the IL-6 (rs2069840 and rs2069833), ESR (rs9340799, rs1256049, rs4986938, and rs2234693), VDR (rs739837 and rs2228570), and 5-HTT genes (rs1042173 and rs38133034) were genotyped by real-time polymerase chain reaction from oral mucosa cells collected. Associations between MIH and SNPs genotypes (recessive and dominant models) and allele frequencies were tested using the chi-square test. Odds ratio (OR) and confidence intervals (CI) were calculated. A significance level of 5% was adopted. Genotypes were tested by the Hardy-Weinberg Equilibrium using chi-square.</p><p><strong>Results: </strong>In rs4986938 (ESR2 gene), children with CT/TT presented significantly lower odds of MIH than CC (OR=0.57, CI 95% [0.35-0.92]). There was no significant association between MIH and other evaluated genes.</p><p><strong>Conclusion: </strong>The genetic polymorphism in the ESR gene is associated with MIH, suggesting that MIH etiology presents a polygenetic involvement.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"33 ","pages":"e20250074"},"PeriodicalIF":2.2,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144690380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of non surgical periodontal therapy on glycemic control in individuals with type 2 diabetes mellitus and periodontitis.","authors":"Rafael Paschoal Esteves Lima, Karolina Skarlet Silva Viana, Pierre Geraldo Braz da Silva Junior, Luís Otávio Miranda Cota, Fernando Oliveira Costa","doi":"10.1590/1678-7757-2025-0121","DOIUrl":"10.1590/1678-7757-2025-0121","url":null,"abstract":"<p><strong>Objective: </strong>The infectious and inflammatory process of the periodontal tissues can contribute to hyperglycemia in patients with diabetes. The objective of this study was to evaluate the effect of non-surgical periodontal therapy on glycemic control in individuals with type 2 diabetes mellitus.</p><p><strong>Methodology: </strong>In this clinical trial with two months of follow-up, 31 participants were included, with 15 having adequate glycemic control and 16 inadequate glycemic control. The participants underwent non-surgical periodontal therapy. Biological, social, and behavioral variables were collected. Periodontal clinical examination was performed at baseline and two months after the intervention. Laboratory tests to assess serum levels of glycated hemoglobin, fasting glucose, and C-reactive protein were requested for all participants at baseline and two months after periodontal treatment.</p><p><strong>Results: </strong>The difference in glycated hemoglobin levels between baseline and two months after non-surgical periodontal therapy was statistically significant in the total sample (p=0.045) and in the group of individuals with adequate glycemic control (p=0.016). No significant difference was observed in glycated hemoglobin levels in the group of individuals with inadequate glycemic control. No significant variation was observed in fasting glucose and C-reactive protein levels after treatment in the studied sample. A reduction in probing depth, gingival inflammation, and gain in clinical attachment was observed in the total sample and in both groups according to glycemic control.</p><p><strong>Conclusion: </strong>Periodontal intervention may contribute to improved glycemic control in individuals with type 2 diabetes mellitus and periodontitis (Brazilian Clinical Trials Registry RBR-9fvwk4m).</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"33 ","pages":"e20250121"},"PeriodicalIF":2.6,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12296231/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144642651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Orofacial myofunctional and anthropometric characteristics of children with and without microcephaly: a case-control study.","authors":"Andréa Monteiro Correia Medeiros, Gabriela Rodrigues Dourado Nobre, Geyse do Espírito Santo Rezende, Íkaro Daniel de Carvalho Barreto, Jonan Emi Valencia Cardenas, Sarah Catarina Santos Nascimento, Anna Luiza Dos Santos Matos, Asenate Soares de Matos Pereira, Ricardo Queiroz Gurgel, Giédre Berretin-Felix","doi":"10.1590/1678-7757-2024-0473","DOIUrl":"10.1590/1678-7757-2024-0473","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to describe and compare morphofunctional orofacial aspects between subjects with and without Zika virus-related microcephaly.</p><p><strong>Methodology: </strong>This was a descriptive, cross-sectional, case-control study with both qualitative and quantitative components. All subjects were born between 2015 and 2016, during the Zika virus outbreak in the Northeast region of Brazil. A total of 48 children were included: 24 with Zika-related microcephaly (MG) and 24 without the condition (CG). We performed the Preliminary Expanded Protocol of Orofacial Myofunctional Evaluation with Scores (OMES-E) for all subjects. Orofacial anthropometric measurements were obtained from 36 of the 48 participants, including 18 from the MG and 18 from the CG.</p><p><strong>Results: </strong>We found lower swallowing efficiency scores in children with microcephaly aged 13-18 months. Significant differences (p<.001) were found between the MG and CG for scores related to the face, cheeks, and total stomatognathic functions. When stratified by age group, differences (p<.001) were found in total scores between MG and CG subjects in the age groups up to 24 months. We found lower scores in the 13-18-month group with microcephaly for swallowing efficiency: 1.3 (SD: .8) versus 5.3 (SD: 1.2); and in the 19-24-month group; for bite: 1 (SD: 0) and 3.9 (SD: .3), and 1.9 (SD: 2.7) and 9.5 (SD: .9); in addition to facial changes: 9.8 (SD: 1.2) and 11.8 (SD: .6). Differences were found in anthropometric orofacial measurements for the upper third of the face (d=-1.215, p<.001) (MG<CG); proportion between the upper third/middle third (d=.463, p=.018) (MG<CG); and upper lip and philtrum (MG>CG) (d=-.679, p<.001).</p><p><strong>Conclusion: </strong>Subjects with microcephaly had altered orofacial myofunction, especially related to swallowing and chewing difficulties in early ages.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"33 ","pages":"e20240473"},"PeriodicalIF":2.6,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12296225/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144608411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of a low-dose combination therapy of zoledronate and dexamethasone on post-tooth extraction socket healing: a pre-clinical study in mice.","authors":"Ana Julia Moreno Barreto, Claudia Cristina Biguetti, Raquel Barroso Parra da Silva, Letícia Citelli Conti, Rafael Carneiro Ortiz, Alaide Gonçalves, Edilson Ervolino, Antonio Hernandes Chaves-Neto, Mariza Akemi Matsumoto","doi":"10.1590/1678-7757-2024-0574","DOIUrl":"https://doi.org/10.1590/1678-7757-2024-0574","url":null,"abstract":"<p><strong>Background: </strong>Pharmaceutical agents targeting distinct therapeutic pathways can differently influence bone metabolism, notably exemplified by nitrogen-containing bisphosphonates and glucocorticoids.</p><p><strong>Objective: </strong>This investigation sought to elucidate the effects of a low-dose administration of zoledronate (ZL) and dexamethasone (DX) on post-tooth extraction sockets healing in a murine model.</p><p><strong>Methodology: </strong>In total, 40 young male C57BL/6J mice were assigned to four distinct groups by weight-stratified randomization: Control (C) - 0.9% saline solution, ZL - 0.05 mg/kg ZL, DX - 5 mg/kg DX, and ZL+DX - combined regimen of 0.05 mg/kg ZL and 5 mg/kg DX. All substances were intraperitoneally delivered on a weekly basis from four weeks before right upper incisor extraction and up to seven and 30 days after it, when blood was collected for biochemical analysis of bone markers and the maxillae were removed and prepared for microcomputed analysis of the trabecular architecture of the healing sockets and to set histological slices to be stained with hematoxylin and eosin and immunohistochemistry for TRAP and Runx2.</p><p><strong>Results: </strong>Histopathology and microCT showed that DX administration correlated with impaired bone formation, manifesting as reduced bone volume/total volume and trabecular thickness. Conversely, ZL exposure disrupted bone viability. However, the combination of both showed enhanced maturation in bone remodeling at day 30. Notably, DX treatment notably reduced serum calcium and phosphate levels and total TRAP. Runx-2+ cells significantly increased in the Control group at day seven when compared to ZL and DX-ZL and at day 30 when compared to ZL.</p><p><strong>Conclusions: </strong>Our findings showed that co-administering low doses of ZL and DX in young male mice augmented the recuperative processes of their post-extraction sockets when juxtaposed with either agent in isolation. Nevertheless, further comprehensive inquiries are needed to delineate the precise underlying mechanisms in a more controlled experimental context.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"33 ","pages":"e20240574"},"PeriodicalIF":2.2,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144608454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of molar incisor hypomineralisation severity on dental hypersensitivity, anxiety/fear, and aesthetic self-perception: a cross-sectional study.","authors":"Bianca Caroline Gomes, Lana Cardoso-Silva, Beatriz Kelly Barros Lopes, Roberta Paula de Faria Melo, Isabella Silva Catananti, Alexandra Mussolino de Queiroz, Francisco Wanderley Garcia Paula-Silva, David John Manton, Fabrício Kitazono de Carvalho","doi":"10.1590/1678-7757-2024-0159","DOIUrl":"10.1590/1678-7757-2024-0159","url":null,"abstract":"<p><strong>Background: </strong>Molar-incisor hypomineralisation (MIH) significantly impacts children's quality of life due to dental hypersensitivity, anxiety, and aesthetic concerns. Few studies have investigated these factors within the same sample, comparing varying levels of MIH severity to MIH-free controls.</p><p><strong>Objectives: </strong>This study evaluated dental hypersensitivity, anxiety, and aesthetic self-perception in children with mild MIH, severe MIH, and controls, and explored correlations between the studied variables.</p><p><strong>Methodology: </strong>A total of 605 students from a single school were evaluated: 110 children with MIH and 214 controls matched by age and sex. MIH severity was clinically assessed using the Ghanim criteria. Hypersensitivity was measured with the visual analogue scale (VAS) and the Schiff Cold Air Sensitivity Scale (SCASS). Anxiety and aesthetic concerns were assessed using the CFSS-DS and CQATA questionnaires, respectively.</p><p><strong>Results: </strong>Overall, 78 children had mild MIH and 32 had severe MIH. Median for dental hypersensitivity (p<0.001) and aesthetic self-perception (p=0.002) were significantly higher in the severe MIH group compared to both the control and mild MIH groups. No differences were found for anxiety. Elevated VAS-measured dental hypersensitivity levels and impaired self-perceived aesthetics were significantly correlated with greater MIH severity (p<0.05). Spearman's correlation analysis further revealed significant positive correlations between anxiety/fear and VAS scores (p=0.023, r=0.239), between anxiety/fear and aesthetic self-perception scores (p=0.007, r=0.282), and between dental appearance classification and VAS hypersensitivity scores (p=0.035, r=0.222). In contrast, SCASS hypersensitivity scores did not significantly correlate with either anxiety/fear or dental appearance classification (p>0.05).</p><p><strong>Conclusions: </strong>Children with severe MIH showed higher dental hypersensitivity and greater perceived aesthetic impairment than children with mild MIH or without MIH.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"33 ","pages":"e20240159"},"PeriodicalIF":2.2,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12269961/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144608410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determination of sexual dimorphism with CBCT images of the frontal sinus using a predictive formula and an artificial neural network.","authors":"Julyana de Araújo Oliveira, Natália Rogério Borella, Flávia Maria de Moraes Ramos-Perez, Andrea Dos Anjos Pontual, Maria Alice Andrade Calazans, Felipe Alberto Barbosa Simão Ferreira, Francisco Madeiro, Maria Luiza Dos Anjos Pontual","doi":"10.1590/1678-7757-2025-0049","DOIUrl":"10.1590/1678-7757-2025-0049","url":null,"abstract":"<p><strong>Objective: </strong>this study aims to evaluate the sexual dimorphism of the morphometric features of the frontal sinus via cone beam computed tomography (CBCT) reconstructions, using a predictive formula and an artificial neural network (ANN).</p><p><strong>Methodology: </strong>the morphometric features of the frontal sinuses obtained from 1,000 CBCT scans, equally distributed by sex, were assessed by two examiners. The frontal sinus morphometric features from 800 CBCT scans were analyzed using Mann-Whitney tests and a multivariate logistic regression model to identify key morphometric features for sex determination and to develop the predictive formula. These features were subsequently used to validate the predictive formula and the machine learning-based classification system. The predictive formula was evaluated using a set of 200 CBCT scans. The machine learning-based classification system consisted of a three-layer ANN trained with 80% of the CBCT scans and tested with the remaining 20%.</p><p><strong>Results: </strong>Except for the higher frontal sinus index in females, males exhibited higher numerical values for height, width, and anteroposterior (AP) length. The significance level for all statistical tests was set at 0.05. Multivariate logistic regression identified the following four essential morphometric features: sinus height, anteroposterior length (depth) of the sinus, sinus width, and total sinus width. Both the predictive formula and the ANN demonstrated sexual dimorphism. The accuracy, specificity, sensitivity, precision, and F1- score values were 73.50%, 74.00%, 73.00%, 73.74%, and 73.37% for the regression model, and 76.00%, 84.00%, 68.00%, 80.95%, and 73.91% for the ANN, respectively. Except for sensitivity, the ANN outperformed the predictive formula regarding maximum specificity, accuracy, precision, and F1 score.</p><p><strong>Conclusion: </strong>both methods, particularly the ANN, can potentially support sex estimation in the Brazilian forensic context.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"33 ","pages":"e20250049"},"PeriodicalIF":2.2,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12269960/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144560216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Is there an association between molar-incisor hypomineralization and carious lesions in seven to ten-year-old Brazilian schoolchildren? A cross-sectional study.","authors":"Karina Kendelhy Santos, Patrícia Gomes Fonseca, Maria Letícia Ramos-Jorge, Maria Eliza da Consolação Soares, Izabella Barbosa Fernandes","doi":"10.1590/1678-7757-2024-0538","DOIUrl":"10.1590/1678-7757-2024-0538","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate whether the presence of molar-incisor hypomineralization (MIH) is associated with a greater number of decayed teeth in schoolchildren.</p><p><strong>Methodology: </strong>A cross-sectional study was conducted with a random sample of 347 children aged from seven to ten years in the Brazilian city of Diamantina. The International Caries Detection and Assessment System (ICDAS) criteria were used to determine the number of teeth with moderate/extensive caries (ICDAS codes 3-6). MIH was assessed according to the criteria of the European Academy of Paediatric Dentistry (EAPD). Sociodemographic data and data regarding children's habits were obtained via a questionnaire sent to parents/guardians. Descriptive analyses, Mann Whitney and Kruskal Wallis tests, and Poisson regression were performed.</p><p><strong>Results: </strong>The prevalence of MIH was 20.5% and that of moderate/extensive caries was 39.2%. The mean number of teeth with moderate/extensive caries was 1.80 (SD±2.67). The mean number of decayed permanent teeth was 0.69 (SD=1.21), and the mean number of decayed primary teeth was 1.11 (SD=1.89). The number of teeth with moderate/extensive caries was associated with the presence of MIH in children (PR=1.45; 95% CI=1.03-2.04; p=0.031). Furthermore, the number of teeth with moderate/extensive caries was associated with lower monthly family income, high frequency of sugar consumption, and visible plaque (p<0.05).</p><p><strong>Conclusion: </strong>The presence of MIH is associated with a greater number of decayed teeth in schoolchildren.</p><p><strong>Clinical relevance: </strong>This study suggests that children with MIH are at higher risk of developing caries, emphasizing the importance of specific preventive care and early treatments for this condition. This can influence clinical practices, public health policies, and parental education.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"33 ","pages":"e20240538"},"PeriodicalIF":2.2,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12269962/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144560218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ERRATUM.","authors":"","doi":"10.1590/1678-7757-2025er003","DOIUrl":"10.1590/1678-7757-2025er003","url":null,"abstract":"<p><p>[This corrects the article doi: 10.1590/1678-775720150393ar].</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"33 ","pages":"e2025er003"},"PeriodicalIF":2.2,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12269959/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144560217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"M1 and M2 macrophages markers are alternately expressed during periapical lesion development.","authors":"Carolina Maschietto Pucinelli, Paulo Nelson-Filho, Marília Pacífico Lucisano, Jorge Esquiche León, Lúcia Helena Faccioli, Carlos Arterio Sorgi, Clara Marina Pereira Cavalcanti Silva, Lea Assed Bezerra da Silva, Raquel Assed Bezerra da Silva","doi":"10.1590/1678-7757-2024-0579","DOIUrl":"10.1590/1678-7757-2024-0579","url":null,"abstract":"<p><strong>Background: </strong>This study evaluated the altered expression levels of genes and cytokines associated with M1 and M2 macrophages during the development of periapical lesion (PL).</p><p><strong>Methodology: </strong>PL was induced in the lower first molars of 96 mice. After the experimental periods of two, seven, 14, 21, and 42 days, the animals were euthanized and their jaws were dissected and submitted to the following analyses: microscopic descriptive analysis and fluorescence microscopy morphometry of PL size (mm2); quantitative gene expression analysis by qRT-PCR for M1 (Cxcl10, Cxcl9, and Nos2) and M2 phenotypes (Arg1, Fizz1, Ym1, and Mrc1); and M1- (GM-CSF, IFN-γ, IL-6, IL-1β, TNF-α) and M2- (IL-4, IL-13, and IL- 10) related cytokines quantification by Luminex. Data were statistically compared by ANOVA, Tukey post-test, Kruskal-Wallis, and Dunn post-test (α=5%).</p><p><strong>Results: </strong>PL area and inflammatory infiltrate increased over experimental periods. From a contextual view, a pro-inflammatory profile and a higher activation of M1 phenotype markers in the initial periods of two and seven days could be observed. On day 21, microscopic features and M2 subtype predominance indicated a repair attempt. However, on day 42, an acute exacerbation of immunoinflammatory process and return to the M1 macrophage profile were evidenced.</p><p><strong>Conclusion: </strong>M1 and M2 macrophage polarization-related markers were expressed alternately throughout the experimental periods, according to the stage of PL progression.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"33 ","pages":"e20240579"},"PeriodicalIF":2.6,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12296229/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144560219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}