Irisvaldo Lima Guedes, Matheus Oliveira do Nascimento, Leandro de Sousa Dias, Alyne Rodrigues de Araujo-Nobre, Humberto Medeiros Barreto, Érika de Araújo Abi-Chacra, Ana Cristina Vasconcelos Fialho, Gláuber Campos Vale, André Luis Menezes Carvalho
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引用次数: 0
Abstract
Background: This article is derived from Irisvaldo Lima Guedes's Master's dissertation and is available at the address: https://sigaa.ufpi.br/sigaa/public/programa/noticias_desc.jsf?lc=pt_BR&id=370¬icia=519307121 Eugenol has demonstrated efficacy against Candida spp., which is highly prevalent in denture wearers. However, the low water solubility and high volatility limit its application. The encapsulation in nanostructured lipid carriers (NLCs) may be a viable approach for developing new sanitizing agents for denture hygiene.
Objective: To develop a sanitizing dispersion for denture hygiene using nanostructured lipid carriers (NLCs) containing eugenol and to evaluate the efficacy against Candida spp. biofilms.
Methodology: The formulation was prepared using the ultrasonication method and characterized in terms of particle size (PS), polydispersity index (PDI), zeta potential (ZP), and encapsulation efficiency (EE). The minimum inhibitory concentration (MIC) was determined by the broth microdilution method and the antifungal activity was evaluated by four treatment groups (nanostructured formulation containing eugenol (NFE), free eugenol (FE), saline solution (SS), and the drug-free formulation NFW after eight hours of immersion in biofilms of two Candida species (Candida albicans and Candida glabrata) adhered to polymethyl methacrylate resin specimens.
Results: The nanoparticles of NFE showed a particle size of 199.5±2.55 nanometers (nm) as measured by DLS, high homogeneity (0.07±0.02), an EE of 83.07±0.23, and a negative ZP (-25.86±0.65). The MICs of FE for Candida albicans and Candida glabrata were up to 10 times (64 µg/mL) and eight times (128 µg/mL) higher, respectively, than the MICs of NFE (6 µg/mL and 16 µg/mL). The biofilms of these microorganisms showed a significant reduction after immersion in NFE compared to the other tested groups (FE, NBF, and SS) (P<0.0001).
Conclusion: The NFE demonstrated fungicidal activity against the isolated strains and significantly reduced Candida biofilms, thus showing promising performance for the sanitization of dentures over eight hours.
背景：这篇文章来源于Irisvaldo Lima Guedes的硕士论文，可在以下地址获得：https://sigaa.ufpi.br/sigaa/public/programa/noticias_desc.jsf?lc=pt_BR&id=370¬icia=519307121丁香酚已被证明对假丝酵母菌有效，假丝酵母菌在假牙佩戴者中非常普遍。但其水溶性低、挥发性高，限制了其应用。纳米结构脂质载体（nlc）的包封可能是开发新型义齿卫生消毒剂的一种可行方法。目的：研制一种含丁香酚的纳米结构脂质载体（nnlcs）用于义齿卫生消毒分散剂，并评价其对念珠菌生物膜的抗菌效果。方法：采用超声法制备该制剂，并对其粒径（PS）、多分散性指数（PDI）、ζ电位（ZP）、包封率（EE）进行表征。采用肉汤微量稀释法测定最小抑菌浓度（MIC），采用含丁香酚纳米配方（NFE）、游离丁香酚纳米配方（FE）、生理盐水溶液（SS）和无药配方（NFW） 4个处理组，分别对两种念珠菌（白色念珠菌和光面念珠菌）粘附在聚甲基丙烯酸甲酯树脂标本上的生物膜浸泡8小时，评价其抑菌活性。结果：NFE纳米颗粒经DLS测定粒径为199.5±2.55 nm，均匀性高（0.07±0.02），EE为83.07±0.23，ZP为负（-25.86±0.65）。FE对白色念珠菌和光秃念珠菌的mic分别比NFE（6µg/mL和16µg/mL）高10倍（64µg/mL）和8倍（128µg/mL）。与其他实验组（FE、NBF和SS）相比，NFE浸泡后这些微生物的生物膜明显减少(p结论：NFE对分离菌株具有杀真菌活性，并显著减少念珠菌的生物膜，因此在假牙8小时的消毒中表现出良好的效果。
期刊介绍：
The Journal of Applied Oral Science is committed in publishing the scientific and technologic advances achieved by the dental community, according to the quality indicators and peer reviewed material, with the objective of assuring its acceptability at the local, regional, national and international levels. The primary goal of The Journal of Applied Oral Science is to publish the outcomes of original investigations as well as invited case reports and invited reviews in the field of Dentistry and related areas.
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