The effects of Stemregen® host modulation therapy on experimentally induced apical periodontitis in rats.

IF 2.2 3区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Applied Oral Science Pub Date : 2025-02-03 eCollection Date: 2025-01-01 DOI:10.1590/1678-7757-2024-0446

Fatma Gönüllü, Mevlüt Sinan Ocak, Serkan Dundar, İbrahim Hanifi Özercan

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引用次数: 0

Abstract

Objective: This study evaluated the effect of Stemregen® nutritional supplement on inflammation and resorption in apical periodontitis using a rat model.

Methodology: Rats were divided in three groups: negative control (n=7), positive control (n=10), and Stemregen® (Stem) (n=10). Apical periodontitis was induced in the positive control and Stem groups, and all rats were sacrificed on the 30th day. Serum phosphorus (P), calcium (Ca), and alkaline phosphatase (ALP) were analyzed. Histopathological assessments measured osteoblastic and osteoclastic activity, inflammation, fibrosis, and abscess density. Immunohistochemical analyses evaluated RANKL, TRAP, and OPG levels.

Results: Results showed significantly lower osteoblastic activity in the negative control compared to Stem and positive control groups (p=0.005). Osteoclastic activity was higher in the positive control (p=0.032). Inflammation and abscess formation were reduced in the Stem group compared to the positive control (p<0.001). OPG levels were lower in the negative control compared to the other groups (p=0.005).

Conclusion: Stemregen® effectively reduced inflammation and bone destruction, suggesting potential benefits for apical periodontitis management, though further research is needed.

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Stemregen®宿主调节疗法对实验性大鼠根尖牙周炎的影响。

目的：研究Stemregen®营养补充剂对大鼠根尖牙周炎炎症和吸收的影响。方法：将大鼠分为阴性对照组（n=7）、阳性对照组（n=10）和Stemregen®（Stem）组（n=10）。阳性对照组和干组大鼠均诱发根尖牙周炎，并于第30天处死。测定血清磷(P)、钙（Ca）、碱性磷酸酶（ALP）。组织病理学评估测量成骨细胞和破骨细胞活性、炎症、纤维化和脓肿密度。免疫组化分析评估RANKL、TRAP和OPG水平。结果：阴性对照组的成骨细胞活性明显低于Stem组和阳性对照组（p=0.005）。阳性对照组破骨细胞活性较高（p=0.032）。与阳性对照组相比，Stem组的炎症和脓肿形成减少(结论：Stemregen®有效地减少了炎症和骨破坏，提示根尖牙周炎治疗的潜在益处，但需要进一步的研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Applied Oral Science 医学-牙科与口腔外科

CiteScore

4.80

自引率

3.70%

发文量

审稿时长

4-8 weeks

期刊介绍： The Journal of Applied Oral Science is committed in publishing the scientific and technologic advances achieved by the dental community, according to the quality indicators and peer reviewed material, with the objective of assuring its acceptability at the local, regional, national and international levels. The primary goal of The Journal of Applied Oral Science is to publish the outcomes of original investigations as well as invited case reports and invited reviews in the field of Dentistry and related areas.