Journal of Applied Oral Science最新文献

{"title":"Association between dental fracture and amalgam restoration: a case-control study.","authors":"Luana Dos Santos Souza, Victor Ruano, Rafael Santos Rocha, Eduardo Varanda, Taciana Marco Ferraz Caneppele, Eduardo Bresciani","doi":"10.1590/1678-7757-2024-0467","DOIUrl":"https://doi.org/10.1590/1678-7757-2024-0467","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the possible clinical association between dental fracture and the presence of amalgam restorations, including other restorative treatments in the control group. The potential association of fractures with dental wear facets and the restoration size was also assessed as a secondary objective.</p><p><strong>Methodology: </strong>Patients with fractured teeth restored with silver amalgam or not were included as the case group (n=25). The control group, with non-fractured teeth, was selected after considering the case group aspects, with twice as many patients (n=50) with posterior teeth sound or restored (amalgam, composite resin, or another restorative material). For both groups, the type of restorative material, extension of the restorations, remaining tooth structure, and the presence or absence of wear facets were analyzed. The teeth were impressed with alginate, and from the plaster models, the extent of fractures or restorations was measured by two calibrated examiners with a digital caliper at the cervico-occlusal and bucco-lingual directions. The data were subjected to the Chi-square test (5%) and odds ratio.</p><p><strong>Results: </strong>There was no statistical difference between the presence or absence of amalgam restorations regarding the risk of tooth fracture. Regarding fractures larger than 3.5mm, the chances of failure are 0.53 for amalgam restorations with no statistical differences (p=0.433), and, regarding the presence of wear facets, the odds ratio of failure is 1.357 for amalgam restorations (p=0.65).</p><p><strong>Conclusion: </strong>It can be deduced that, within the conditions of the study, no discernible association exists between dental fractures and the presence of silver amalgam restorations.Clinical Trial Register: (ReBEC) UNT code U1111-1215-7255.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"33 ","pages":"e20240467"},"PeriodicalIF":2.2,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12014111/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143991529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chitosan nanoparticles suspension can minimize enamel loss after in vitro erosive challenge.","authors":"Renata Cristina Sobreira França, Rebeca Tibau Aguiar Dias, Ranam Moreira Reis, Frederico Barbosa de Sousa, Hugo Lemes Carlo, Rogerio Lacerda Dos Santos, Fabíola Galbiatti de Carvalho","doi":"10.1590/1678-7757-2024-0445","DOIUrl":"https://doi.org/10.1590/1678-7757-2024-0445","url":null,"abstract":"<p><strong>Background: </strong>Chitosan nanoparticles suspension (ChNPs) showed antimicrobial effects in the oral cavity, but its effects on enamel erosion prevention remain little explored.</p><p><strong>Objective: </strong>This study synthesized ChNPs and evaluated their effect on enamel after erosive challenge.</p><p><strong>Design: </strong>ChNPs were synthesized by ionic gelation and characterized using scanning electron microscopy (SEM), infrared spectrophotometry (FTIR), dynamic light scattering methods (DLS) and zeta potential (ZP). In total, 56 human enamel blocks were divided into four groups (n=14/group): (i) ChNPs suspension (4.4mg/mL); (ii) 0.05% sodium fluoride solution (NaF); (iii) chitosan solution (5.0 mg/mL); and (iv) distilled water. After incubation in freshly collected human saliva (3h), the samples were exposed to erosive challenge in 1% citric acid (90s) and remineralizing solution (2h) performed four times a day. After the 1st and 4th acid exposures, solutions were applied for 2 min. After 7 days, % Vickers surface hardness change (% SMH) was obtained using 300 g load applied for 15s. Enamel surface loss was evaluated using optical profilometer by subtracting the final profile values from baseline ones. Data were analyzed by ANOVA and post-hoc T tests (α=0.05). Surface topography was obtained by optical profilometer.</p><p><strong>Results: </strong>SEM revealed the formation of spherical nanoparticles. DLS showed nanoparticles with 85.7±10.5 nm diameter and ZP value of +45.5±5.4mV. Enamel surface loss was significantly lower in ChNPs and NaF groups, exhibiting a less rough surface in the treated areas. ChNPs, NaF and chitosan groups showed lower % SMH values.</p><p><strong>Conclusions: </strong>ChNPs suspension minimized enamel loss after in vitro erosive challenge and appears to be a promising material for enamel erosion prevention.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"33 ","pages":"e20240445"},"PeriodicalIF":2.2,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12014109/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143968004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of volumetric wear loss and pain scores of the digitally and conventionally manufactured occlusal splints for individuals with sleep bruxism.","authors":"Sevda Miray Soydaş Smail, Zeliha Şanivar Abbasgholizadeh, Erkut Kahramanoğlu","doi":"10.1590/1678-7757-2025-0052","DOIUrl":"10.1590/1678-7757-2025-0052","url":null,"abstract":"<p><strong>Introduction: </strong>According to the latest international consensus in 2018, sleep bruxism is the activity of the masticatory muscles during sleep characterized by rhythmic or non-rhythmic teeth clenching or grinding. Regarding its harmful effects, bruxism is considered one of the predisposing factors of tooth wear and temporomandibular joint diseases. Occlusal splint therapy is the most frequently used treatment for minimizing these harmful effects.</p><p><strong>Objectives: </strong>This study compared the volumetric wear loss and pain scores between digitally and conventionally manufactured occlusal splints for individuals with sleep bruxism.</p><p><strong>Methodology: </strong>A total of 30 individuals diagnosed with sleep bruxism were selected following the inclusion criteria and randomly divided into two groups. Pain scores were subjectively reported using a visual analog scale. Volumetric wear loss of the occlusal splint surface was measured using the Geomagic software. Data were analyzed with SPSS version 25.0.</p><p><strong>Results: </strong>At the six-month follow-up, conventionally manufactured splints (103.53±41.23) showed a volumetric loss significantly higher than that the digital ones (62.33±26.29) (p=0.005). We found no significant difference between the two splint types regarding VAS scores.</p><p><strong>Conclusion: </strong>Occlusal splint wear can gradually alter the balance of occlusal contact and potentially reduce its therapeutic effectiveness, highlighting the importance of using wear-resistant materials. Our findings indicate that digital manufacturing processes provide advantages due to their long-term clinical outcomes.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"33 ","pages":"e20250052"},"PeriodicalIF":2.2,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12014110/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143764001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective effect of adenosine triphosphate against cisplatin-induced necrotic and degenerative oral mucositis in rats.","authors":"Ismail Salcan, Muhammed Dilber, Zeynep Suleyman, Nurinisa Yucel, Sara Salcan, Sefa Kesan, Gulce Naz Yazici, Fatih Celik, Merve Koseturk, Nurdan Alcan Alp, Halis Suleyman","doi":"10.1590/1678-7757-2025-0007","DOIUrl":"10.1590/1678-7757-2025-0007","url":null,"abstract":"<p><strong>Background: </strong>Inflammation, oxidative damage, and adenosine triphosphate (ATP) depletion play a role in the pathogenesis of cisplatin (CIS)-induced oral mucositis.</p><p><strong>Objective: </strong>The purpose of this research is to examine the impact of ATP against potential oral mucositis development in cisplatin-treated rats. Methodology All rats were randomly assigned to four groups, namely healthy control group (HG), ATP group (ATPG), Cisplatin group (CISG), and ATP + Cisplatin group (ATCS). Firstly, ATP 4 mg/kg was administered via intraperitoneal injection (IP) to both ATPG and ATCS groups. The same volume of normal saline was injected into HG and CISG groups. After 1 h, cisplatin 5 mg/kg was administered via IP to CISG and ATCS groups. The drugs were taken 1x1 for 7 d. Later, tongue tissues were collected from all groups. Biochemical, macroscopic, and histopathological examinations were performed on all tissues.</p><p><strong>Results: </strong>ATP inhibited cisplatin-induced oxidative damage and pro-inflammatory cytokines levels in tongue tissue. In the CIS group, a significant number of distinct sulcus formations were found in the apex and corpus, as well as a few ulcer foci in the corpus, significant papilla loss, and bleeding. Meanwhile, in the ATP group, a similar appearance to healthy tissue was observed. Histopathologically, it was determined that in cisplatin-aggravated tongue tissue damage, filiform papillae decreased when ATP was administered, and the arrangement and structures of the epithelium, blood capillaries, muscle groups, and adipose cell groups were normal.</p><p><strong>Conclusions: </strong>Oral mucositis caused by cisplatin is alleviated by ATP. These findings may be useful for developing new therapeutic approaches to prevent or treat mucositis, a side effect so severe that can lead to treatment discontinuation.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"33 ","pages":"e20250007"},"PeriodicalIF":2.2,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12002740/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143709853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between generalized joint hypermobility, temporomandibular joint hypertranslation and temporomandibular disorders: a scoping review.","authors":"Samilla Pontes Braga, Carolina Ortigosa Cunha, Ambrosina Michelotti, Leonardo Rigoldi Bonjardim, Paulo César Rodrigues Conti","doi":"10.1590/1678-7757-2024-0302","DOIUrl":"10.1590/1678-7757-2024-0302","url":null,"abstract":"<p><p>Generalized Joint Hypermobility (GJH) is one of the pathophysiological contributing factors for the development of temporomandibular disorders (TMD). There are, however, several counterpoints on the potential relation between TMD and joint hypermobility, especially when considering the temporomandibular joint (TMJ), event known as TMJ hypertranslation. Additionally, there is no consensus regarding the clinical and imaging diagnostic criteria for such condition. Hence, this scoping review addresses the association between GJH, TMJ hypertranslation and TMD, highlighting the lack of consensus concerning TMJ hypertranslation diagnosis. Eligibility criteria included book sections, clinical trials, meta-analyses, multicenter studies, observational studies, and reviews published in English between 1964 and 2024. Bibliographic search was conducted on the PubMed, SciELO, LILACS and Science Direct databases using the following Medical Subjective Headings (MeSH) terms: \"temporomandibular joint disorders,\" \"temporomandibular joint,\" \"joint instability\" and \"joint dislocations.\" \"TMJ hypermobility\" and \"TMJ subluxation,\" non-indexed terms, were applied as individual searches in the same databases. Manual search was performed in selected works by cross-referencing the included studies and book sections. Additional search was conducted in the grey literature. All searches were performed from January to June 2024. After selection, 54 texts were included. While some studies suggest that joint hypermobility (generalized or TMJ specific) may be a risk factor for TMD, especially of the intra-articular type, others rule out this association. No consensus on the potential association between joint hypermobility and TMD was achieved due to the diverse methodologies used to define TMJ hypertranslation diagnosis. More robust and controlled studies are needed to establish a diagnostic criteria and, consequently, understanding of its potential repercussions on masticatory structures, as well as management and prevention of the clinical manifestations.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"33 ","pages":"e20240302"},"PeriodicalIF":2.2,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12005711/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143709852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of radiotherapy in chemical composition and mechanical properties of human cervical dentin: an in vitro study.","authors":"Renata Borges Rodrigues, Allyne Jorcelino Daloia de Carvalho, Bruna Vanessa Felipe E Silva, Paulo Cézar Simamoto-Júnior, Veridiana Resende Novais","doi":"10.1590/1678-7757-2024-0279","DOIUrl":"10.1590/1678-7757-2024-0279","url":null,"abstract":"<p><strong>Background: </strong>Ionizing radiation directly affects hard dental tissues, compromising the dental structure, which results in damage to dentin collagen fibers and impacts the integrity of the dentin-enamel junction (DEJ).</p><p><strong>Objective: </strong>To evaluate the effects of radiotherapy on the chemical composition and mechanical properties of human cervical dentin.</p><p><strong>Methodology: </strong>Ten third molars were divided into control/non-irradiated and irradiated groups (n=5). The irradiated teeth were subjected to in vitro radiotherapy with the following protocol: 1.8 Gy daily, five days per week for eight weeks, totaling 72 Gy. The dentin in the cervical region was evaluated for each group. The chemical composition was assessed using Fourier transform infrared spectroscopy (FTIR) and Raman spectroscopy, focusing on the mineral/matrix ratio (M:M), carbonate/mineral ratio (C:M), and amide I/amide III ratio. Amide I/CH2 ratio was used to assess collagen quality, as amide I reflects protein conformation and hydrogen bonding, while CH2 indicates side-chain vibrations with low sensitivity to molecular orientation. Nanohardness and elastic modulus were evaluated by instrumented indentation. Scanning electron microscopy (SEM) was used to assess the enamel's morphology. Statistical analysis of each parameter was performed using a t-test.</p><p><strong>Results: </strong>The FTIR analysis showed statistically significant differences in the C:M ratio (p=0.004) and amide I/amide III ratio (p=0.007). Raman spectroscopy revealed significant differences in the M:M ratio (p<0.001), as well as in the amide I/amide III (p<0.001) and amide I/CH2 ratios (p<0.001). Additionally, nanohardness (p=0.04) and the elastic modulus (p=0.003) showed statistically significant differences. SEM images revealed sound dentin shows normal tissue organization, whereas irradiated dentin showed no clear limit between peri and intertubular dentin.</p><p><strong>Conclusions: </strong>Radiotherapy induced significant changes in dentin composition and mechanical properties, characterized by increased organic content and phosphate levels, reduced carbonate, and decreased nanohardness and elastic modulus. These findings highlight the adverse effects on dentin's structural integrity.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"33 ","pages":"e20240279"},"PeriodicalIF":2.2,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11978287/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143657315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring polymorphisms in genes encoding growth factors associated with non-syndromic cleft lip with or without cleft palate and tooth agenesis.","authors":"Gabriela Fonseca-Souza, Vitória Somma Tessari, Rafaela Scariot, Christian Kirschneck, Ricardo Della Coletta, Erika Calvano Küchler, Juliana Feltrin-Souza","doi":"10.1590/1678-7757-2024-0501","DOIUrl":"10.1590/1678-7757-2024-0501","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the association between non-syndromic cleft lip with or without cleft palate (NSCL±P) and tooth agenesis (TA), as well as the association of both conditions with polymorphisms in genes encoding growth factors.</p><p><strong>Methodology: </strong>This cross-sectional study included children with NSCL±P and a control group of children without NSCL±P. Permanent teeth TA (excluding third molars) was evaluated using panoramic radiographs by a trained examiner. Only TA located outside the cleft was considered in the NSCL±P group. Genetic polymorphisms in Transforming Growth Factor Beta 1 (TGFB1)-rs1800470 and rs4803455-Transforming Growth Factor Beta Receptor 2 (TGFBR2)-rs3087465 and rs764522-Epidermal Growth Factor (EGF)-rs4444903 and rs2237051-and Epidermal Growth Factor Receptor (EGFR)-rs2227983- were genotyped by real-time PCR allele discrimination from buccal cell samples. Associations were tested by uni and multivariable Poisson regression models (5% significance level).</p><p><strong>Results: </strong>A total of 243 children-127 with NSCL±P (mean age = 8.80±2.14 years) and 116 without NSCL±P (mean age = 8.58±2.03 years) were included. TA was more frequent in the NSCL±P group (23.8%) than in the control group (6.2%) (p<0.01). The EGF rs2237051 was significantly associated with NSCL±P, independently of the other variables (PRa=1.41; p=0.042). Regarding TA, only the cleft presence was associated with a higher prevalence of TA regardless of different variables (PRa=3.70; p=0.001). There was no association between TA and the investigated genetic polymorphisms. When TA and NSCL±P were considered together, a borderline association was observed with rs1800470 in TGFB1 (p=0.06).</p><p><strong>Conclusion: </strong>NSCL±P is associated with TA outside the cleft area. The EGF rs2237051 was associated with NSCL±P. Polymorphisms in genes encoding growth factors are not associated with TA.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"33 ","pages":"e20240501"},"PeriodicalIF":2.2,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11978285/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143657328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunomodulatory effects of apical papilla cells on periodontal ligament fibroblasts stimulated with Escherichia coli lipopolysaccharide: an in vitro study.","authors":"Alexandre Guimarães Dos Santos, Karollyne Santos Spigariol, Letícia Martins Santos, Marinella Holzhausen, Carla Renata Sipert","doi":"10.1590/1678-7757-2024-0338","DOIUrl":"10.1590/1678-7757-2024-0338","url":null,"abstract":"<p><strong>Background: </strong>The role of human Stem Cells from the Apical Papilla (SCAP) in tissue regeneration has been described, but their impact on modulating the apical inflammatory process by other surrounding cell populations, such as periodontal ligament fibroblasts (PLFs), is unclear. Therefore, we investigated the role of SCAP in the activation of PLFs in vitro.</p><p><strong>Methods: </strong>Primary SCAP culture was used to obtain conditioned media (CM). A primary human PLF culture was established and stimulated with increasing concentrations of Escherichia coli lipopolysaccharide (LPS) (0.01, 0.1, and 1 µg/mL). At the 24 h time-point, an MTT viability assay was performed, and interleukin (IL)-6 and chemokine (CC-motif) ligand 2 (CCL2) levels were quantified by enzyme-linked immunosorbent assay. Then, PLFs were stimulated with LPS in the presence of SCAP-CM (1:5 dilution) for cell viability assessment and cytokine detection. The following groups were tested: PLF activated with LPS at concentrations of 0.01 and 1 µg/mL with or without SCAP-CM; a group with PLF stimulated by SCAP-CM alone; and a control group (proliferation medium only). The experiments were conducted in triplicate and sextuplicate. Statistical analyses were performed using analysis of variance followed by Tukey's post-hoc test, with statistical significance established at 5% (p=0.05).</p><p><strong>Results: </strong>The MTT assay showed no cytotoxicity of LPS or SCAP-CM on PLFs (p>0.05). The production of CCL2 and IL-6 significantly increased in the presence of SCAP-CM regardless of the presence of LPS (p<0.0001).</p><p><strong>Conclusion: </strong>SCAP-CM significantly enhanced the release of proinflammatory cytokines by PLFs in vitro.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"33 ","pages":"e20240338"},"PeriodicalIF":2.2,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11869941/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143657308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lipid nanocarrier containing eugenol for denture hygiene: evaluation of efficacy against Candida biofilms.","authors":"Irisvaldo Lima Guedes, Matheus Oliveira do Nascimento, Leandro de Sousa Dias, Alyne Rodrigues de Araujo-Nobre, Humberto Medeiros Barreto, Érika de Araújo Abi-Chacra, Ana Cristina Vasconcelos Fialho, Gláuber Campos Vale, André Luis Menezes Carvalho","doi":"10.1590/1678-7757-2024-0455","DOIUrl":"10.1590/1678-7757-2024-0455","url":null,"abstract":"<p><strong>Background: </strong>This article is derived from Irisvaldo Lima Guedes's Master's dissertation and is available at the address: https://sigaa.ufpi.br/sigaa/public/programa/noticias_desc.jsf?lc=pt_BR&id=370&noticia=519307121 Eugenol has demonstrated efficacy against Candida spp., which is highly prevalent in denture wearers. However, the low water solubility and high volatility limit its application. The encapsulation in nanostructured lipid carriers (NLCs) may be a viable approach for developing new sanitizing agents for denture hygiene.</p><p><strong>Objective: </strong>To develop a sanitizing dispersion for denture hygiene using nanostructured lipid carriers (NLCs) containing eugenol and to evaluate the efficacy against Candida spp. biofilms.</p><p><strong>Methodology: </strong>The formulation was prepared using the ultrasonication method and characterized in terms of particle size (PS), polydispersity index (PDI), zeta potential (ZP), and encapsulation efficiency (EE). The minimum inhibitory concentration (MIC) was determined by the broth microdilution method and the antifungal activity was evaluated by four treatment groups (nanostructured formulation containing eugenol (NFE), free eugenol (FE), saline solution (SS), and the drug-free formulation NFW after eight hours of immersion in biofilms of two Candida species (Candida albicans and Candida glabrata) adhered to polymethyl methacrylate resin specimens.</p><p><strong>Results: </strong>The nanoparticles of NFE showed a particle size of 199.5±2.55 nanometers (nm) as measured by DLS, high homogeneity (0.07±0.02), an EE of 83.07±0.23, and a negative ZP (-25.86±0.65). The MICs of FE for Candida albicans and Candida glabrata were up to 10 times (64 µg/mL) and eight times (128 µg/mL) higher, respectively, than the MICs of NFE (6 µg/mL and 16 µg/mL). The biofilms of these microorganisms showed a significant reduction after immersion in NFE compared to the other tested groups (FE, NBF, and SS) (P<0.0001).</p><p><strong>Conclusion: </strong>The NFE demonstrated fungicidal activity against the isolated strains and significantly reduced Candida biofilms, thus showing promising performance for the sanitization of dentures over eight hours.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"33 ","pages":"e20240455"},"PeriodicalIF":2.2,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12002739/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143639456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ERRATUM.","authors":"","doi":"10.1590/1678-7757-2024er002","DOIUrl":"10.1590/1678-7757-2024er002","url":null,"abstract":"<p><p>[This corrects the article doi: 10.1590/1678-7757-2024-0245].</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"33 ","pages":"e2024er002"},"PeriodicalIF":2.2,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11869940/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143501243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}