Journal of Applied Oral Science最新文献

{"title":"Polyphenol-functionalized silver nanoparticles promote differential remineralization and reinforcement of demineralized dentin.","authors":"Tattiana Enrich, Santiago González-López, Alejandro B Rodríguez-Navarro, María V Bolaños-Carmona, Carolina Cifuentes-Jiménez","doi":"10.1590/1678-7765-2025-0848","DOIUrl":"10.1590/1678-7765-2025-0848","url":null,"abstract":"<p><strong>Objectives: </strong>This study aimed to synthesize and characterize AgNPs functionalized with crosslinking polyphenols extracted from grape seed extract (GSE) and green tea leaves (GT), and to evaluate their biocompatibility and their effects on the mechanical and physicochemical properties of demineralized dentin.</p><p><strong>Methodology: </strong>AgNPs were characterized by TEM, XRD, TGA, and zeta potential analysis to determine particle morphology, crystalline structure, chemical composition, and surface charge. Biocompatibility was assessed using fibroblast cytotoxicity assays. In total, 40 human mid-coronal dentin specimens were randomly assigned to four groups (n=10): Control- (sound dentin), Control+ (pH-cycled dentin without treatment), AgGSE (pH-cycled + AgGSE, 1 min), and AgGT (pH-cycled + AgGT, 1 min). Treated dentin was analyzed by ATR-FTIR, XRD, TGA, and three-point bending tests.</p><p><strong>Results: </strong>Both AgNPs were successfully synthesized and exhibited high biocompatibility. AgGSE demonstrated greater dentin matrix interaction, resulting in significantly higher flexural strength (p≤0.001). In contrast, AgGT induced pronounced remineralization with increased PO4/Amide I ratio (p=0.003) and CO3/PO4 ratios (p=0.007 by TGA; p=0.03 by ATR-FTIR), consistent with carbonated apatite deposition.</p><p><strong>Conclusions: </strong>Green-synthesized AgNPs functionalized with GT or GSE promoted reinforcement and remineralization of demineralized dentin through distinct mechanisms of action. These findings highlight their potential as biocompatible agents for dental therapies.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"34 ","pages":"e20250848"},"PeriodicalIF":3.0,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13290210/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147838127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Statin-loaded carbonated hydroxyapatite nanoemulsion controls matrix metalloproteinase-1 activity to enhance post-orthodontic stability in rats.","authors":"Ananto Ali Alhasyimi, Niswati Fathmah Rosyida, Pinandi Sri Pudyani","doi":"10.1590/1678-7765-2026-0023","DOIUrl":"10.1590/1678-7765-2026-0023","url":null,"abstract":"<p><strong>Background: </strong>The persistence of periodontal ligament (PDL) and alveolar bone remodeling following appliance removal is a key factor in orthodontic relapse, which is a significant barrier to treatment stability. In this study, a statin-loaded carbonated hydroxyapatite (CHA) nanoemulsion was evaluated as a local therapy to control relapse rate by modulating matrix metalloproteinase-1 (MMP-1) in rats.</p><p><strong>Methodology: </strong>A total of 48 rats (n=48) were allocated to control, simvastatin, CHA hydrogel, or CHA-simvastatin nanoemulsion groups. Treatments were delivered intrasulcularly during stabilization, and relapse was measured at days zero, one, seven, and 14 post-debonding. MMP-1 expression in the PDL was assessed using immunofluorescence at the same time points. Collected data were evaluated using analysis of variance.</p><p><strong>Results: </strong>Relapse occurred in all groups, with the highest relapse rate during early observation. At day one, the relapse rate was significantly reduced in treatment groups compared with the control, and the CHA-simvastatin group showed the lowest relapse rate (control 1048±20.20 vs. CHA-simvastatin 549.33±27.22 μmd-1; p=0.001). MMP-1 expression peaked at day one and was significantly lower in all treatment groups than control, with the greatest suppression in the CHA-simvastatin group (16.33±2.08 vs. 7.33±0.57 positive cells/field; p=0.001). By day 14, relapse rates and MMP-1 expression decreased across groups, with persisting advantages for the combined treatment.</p><p><strong>Conclusion: </strong>Local CHA-simvastatin nanoemulsion decreased early relapse and downregulated MMP-1 expression in rats, suggesting a promising adjunctive strategy to enhance post-orthodontic stability.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"34 ","pages":"e20260023"},"PeriodicalIF":3.0,"publicationDate":"2026-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13123790/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147772450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of root coverage by injectable-platelet rich fibrin in thick and thin phenotypes: a parallel-armed, prospective, preliminary clinical study with up to 48 months of follow-up.","authors":"Berceste Guler Ayyildiz, Busra Terzioglu, Seyma Eken","doi":"10.1590/1678-7765-2025-0702","DOIUrl":"10.1590/1678-7765-2025-0702","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to compare the effectiveness of injectable platelet-rich fibrin (I-PRF) using a submucosal injection technique for creeping attachment in thin and thick phenotypes with gingival recession.</p><p><strong>Methodology: </strong>The study was designed as a prospective clinical trial. Participants were divided into two groups based on gingival thickness (GT): thin phenotype group (≤1.0 mm) and thick phenotype group (>1.0 mm). I-PRF was applied once a month to each gingival site using the submucosal injection technique, for a total of three applications. Periodontal parameters were measured at baseline (T0), nine months after treatment (T1), and 12-48 months after treatment (T2). Root coverage percentage (RC%) was calculated.</p><p><strong>Results: </strong>Twelve systemically healthy, non-smoking patients (seven males and five females; mean age: 36.5±9.4), comprising 66 teeth, were analyzed. The primary outcome, RC%, demonstrated a statistically significant intergroup difference at T1 (p=0.032); however, this difference was not statistically significant at T2. In the thick phenotype group, RC% was 20.91±30.80% in T1 and increased to 31.40±30.92% in T2 (p=0.040). Conversely, the thin phenotype group showed higher RC% values at T1 (35.04±29.76%), which were not sustained at T2 (30.70±28.46%; p=0.355). Recession depth decreased in both groups at all time points, but this difference was not statistically significant. Keratinized tissue width showed a significant increase in both groups, from 2.34±1.08 and 2.73±0.95 at T0, respectively, to 2.89±1.17 and 3.28±1.12 at T1 (p<0.05). In the intergroup comparison, GT values were significantly higher in the thick phenotype group than in the thin phenotype group at T0 and T2, while no significant difference was observed at T1.</p><p><strong>Conclusion: </strong>I-PRF application was shown to enhance RC% in both phenotypes. A significant increase in GT was observed in the thin phenotype; however, these increases in GT and RC% were not fully sustained during long-term follow-up in this sample.Clinical Trial registration: NCT05591326.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"34 ","pages":"e20250702"},"PeriodicalIF":3.0,"publicationDate":"2026-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13123792/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147772356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression of caspase-8 in non-alcoholic fatty liver disease related to ligature-induced periodontitis in rats.","authors":"Felipe Rodolfo Pereira da Silva, Victor Brito Dantas Martins, Hélio Mateus Silva Nascimento, Paulo Roberto Carneiro Gomes, Nikaely Brandão Barbosa, Vinícius da Silva Caetano, Ivana Márcia Alves Diniz, Karine Duarte da Silva, Any Carolina Cardoso Guimarães Vasconcelos, Daniel Fernando Pereira Vasconcelos","doi":"10.1590/1678-7765-2025-0717","DOIUrl":"10.1590/1678-7765-2025-0717","url":null,"abstract":"<p><strong>Aim: </strong>This study aimed to evaluate the oral and hepatic changes, as well as the expression of caspase-8 in liver tissue damage, both associated with ligature-induced periodontitis in rats.</p><p><strong>Methodology: </strong>A total of 16 female Wistar rats were divided into two groups (n=8): one received ligature around the first lower molar to promote periodontitis, and the other was the control group with no ligature. After the period of induction of the disease the animals were evaluated by clinical measures for periodontitis, euthanized, and the samples of gingival tissue, hepatic tissue, and serum were collected to the measurement of biomarkers for inflammation (myeloperoxidase), oxidative stress (glutathione, malonaldehyde, and nitrate [NO3]), liver damage and histopathological evaluation with immunohistochemistry for caspase-8. The data were expressed as mean and standard deviation for data with normal distribution or median and interquartile range for data with non-normal distribution. We used ANOVA followed by the Student-Newman-Keuls test for multiple comparisons of normally distributed data, and the Kruskal-Wallis test followed by Dunn's test for non-normally distributed data. The statistical tests were performed with GraphPad Prism Software (version 5.0), in which a p-value <0.05 was considered significant.</p><p><strong>Results: </strong>Our study demonstrates that the group with ligature-induced periodontitis showed increased measurement of periodontal destruction, local and systemic biomarkers of inflammation as well as liver damage which we observed several hepatocytes with loss of conformation and steatosis in the periodontitis group. The histopathological evaluation evidenced the periodontitis-related steatosis and higher expression of Caspase-8 in comparison with the control group (p<0.0006).</p><p><strong>Conclusion: </strong>Our study demonstrates the high expression of Caspase-8 in liver damage related to ligature-induced periodontitis in rats.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"34 ","pages":"e20250717"},"PeriodicalIF":3.0,"publicationDate":"2026-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13123789/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147592396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Can angiotensin II affect the inflammatory response of human dental pulp cells in vitro?","authors":"Bella Luna Colombini-Ishikiriama, Thiago José Dionisio, Thais Francini Garbieri, Rafaela Alves da Silva, Sandra Helena Penha Oliveira, Vanessa Soares Lara, Andrew Seth Greene, Carlos Ferreira Dos Santos","doi":"10.1590/1678-7765-2025-0822","DOIUrl":"10.1590/1678-7765-2025-0822","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to evaluate the capacity of fibroblasts from permanent and deciduous dental pulp to express inflammatory mediators when exposed to bacterial and inflammatory antigens. It also sought to assess the presence of components of the renin-angiotensin system (RAS) and the potential modulatory role of ANG-II in this response.</p><p><strong>Methodology: </strong>Fibroblasts were cultured from the pulp of permanent and deciduous teeth obtained from three adult and four pediatric donors with informed consent. Cells were stimulated with Porphyromonas gingivalis and Escherichia coli lipopolysaccharides (LPS) either alone or in combination with ANG-II and, in some cases, with IL-1β. Gene expression of RAS components and various inflammatory mediators was analyzed by qRT-PCR. ANG-II receptor expression was quantified by flow cytometry.</p><p><strong>Results: </strong>The results showed that both bacterial antigens and IL-1β significantly upregulated the expression of genes encoding key inflammatory mediators. However, ANG-II, either alone or in combination with other antigens, failed to alter the expression levels of these mediators.</p><p><strong>Conclusion: </strong>These findings clearly show that ANG-II has no influence on the expression of inflammatory mediators by human dental pulp fibroblasts under the tested conditions.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"34 ","pages":"e20250822"},"PeriodicalIF":3.0,"publicationDate":"2026-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13123768/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147592461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of impacted mandibular third molars on pathological changes on themselves and adjacent tissues across different age groups: a cross-sectional study.","authors":"Xiaoyue Ge, Yixiao Cui, Ning Li, Tianyang Lv, Yuntong Liang, Haochen Wu, Yuxuan Gu, Meiting Li, Changkui Liu, Yayuan Guo, Kaijin Hu","doi":"10.1590/1678-7765-2025-0677","DOIUrl":"10.1590/1678-7765-2025-0677","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the effects of different types of impacted mandibular third molar (IMTM) on lesions in situ and on adjacent tissues at different ages.</p><p><strong>Methodology: </strong>Cone Beam Computed Tomography (CBCT) images of 1007 IMTM cases were retrospectively analyzed. Associations between age, gender, impaction type, distal alveolar bone condition of mandibular second molar (MSM), IMTM-MSM contact status and occurrence of lesions on IMTM and its adjacent tissues were evaluated using Pearson's chi-squared test and Fisher's exact test.</p><p><strong>Results: </strong>MSM distal alveolar bone defects showed the highest incidence among all IMTM-related lesions (79.2%), followed by MSM distal external root resorption (ERR) (25.5%) and MSM distal caries (10.4%). Mesioangular (96.2%, p<0.001) and horizontal (95.9%, p<0.001) IMTM were associated with more severe bone defects and the incidence increased with age. IMTM-MSM surface-to-surface contact was significantly associated with MSM distal caries severity (12.5%, p<0.001) and distal ERR (29.5%, p<0.001). Inflammatory resorption of MSM distal alveolar bone was significantly correlated with MSM distal caries (47.1%, p<0.001), ERR (36.5%, p<0.05), distal alveolar bone defects (100%, p<0.001), and IMTM caries (14.1%, p<0.05). Study design followed the STROBE guidelines.</p><p><strong>Conclusion: </strong>Mesioangular impaction, horizontal impaction, MSM-IMTM surface-to-surface contact, and MSM distal alveolar bone in a state of inflammatory resorption greatly affect lesions on IMTM and its surrounding tissues. These findings suggest the prophylactic extraction in young adults (18-25 years) with high-risk impactions, while recommending observation or cautious management for asymptomatic older patients without signs of inflammatory bone resorption.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"34 ","pages":"e20250677"},"PeriodicalIF":3.0,"publicationDate":"2026-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13123791/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147592474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cognitive and Orofacial Intersections: prevalence of temporomandibular disorders and bruxism in individuals with symptoms of attention deficit hyperactivity disorder.","authors":"Arthur Alexis Valentin Caron, Rodrigo Lorenzi Poluha, Marlon Ferreira Dias, Dyanne Medina Flores, Alfonso Sanchéz-Ayala, Justin Durham, Giancarlo De la Torre Canales","doi":"10.1590/1678-7765-2025-0771","DOIUrl":"10.1590/1678-7765-2025-0771","url":null,"abstract":"<p><strong>Objective: </strong>Despite emerging evidence pointing to a comorbidity between temporomandibular disorders (TMD), bruxism, and neuropsychiatric conditions such as Attention-Deficit/Hyperactivity Disorder (ADHD), limited studies have explored this relationship in adult populations. This study aimed to investigate the prevalence of painful TMD and self-reported bruxism in adults with highly consistent ADHD symptoms.</p><p><strong>Methodology: </strong>In total, 90 adult participants were divided into two groups based on the Adult ADHD Self-Report Scale Screener, G1: individuals with highly consistent ADHD symptomatology (n=60), and G2: controls (n=30). All participants completed validated instruments assessing TMD (TMD Pain Screener), self-reported bruxism (OBC), anxiety (GAD-7), somatization (PHQ-15), perceived stress (PSS), sleep quality (PSQI), pain hypervigilance (PVAQ), and jaw function (JFLS-8). The chi-square test, adjusted using Bonferroni criteria for multiple comparisons, and the Mann-Whitney U-test were used.</p><p><strong>Results: </strong>A higher prevalence of women (p=0.006), South Americans (p=0.008), and individuals with low/lower middle income (p=0.0001) was found in G1. G1 showed a significantly higher prevalence of painful TMD (p=0.001) and awake bruxism (p=0.013) compared to G2. Participants in G1 also exhibited significantly higher levels of anxiety, somatization, stress, poor sleep quality, pain hypervigilance, and jaw limitation (p<0.05). No significant differences in sleep bruxism were found between groups (p>0.05).</p><p><strong>Conclusion: </strong>These findings suggest a potential behavioral and emotional vulnerability to orofacial disorders in adults with highly consistent ADHD symptoms.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"34 ","pages":"e20250771"},"PeriodicalIF":3.0,"publicationDate":"2026-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13123769/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147512342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Apical delta filling with calcium silicate and epoxy resin sealers using different obturation techniques by microcomputed tomography.","authors":"Washington Soares Dos Santos, Jefferson Augusto, Angelo José Sócrates Torres-Carrillo, Igor Bassi Ferreira Petean, Antonio Miranda da Cruz-Filho, Manoel Damião de Sousa-Neto, Fabiane Carneiro Lopes-Olhê, Jardel Francisco Mazzi-Chaves","doi":"10.1590/1678-7765-2025-0774","DOIUrl":"10.1590/1678-7765-2025-0774","url":null,"abstract":"<p><strong>Objectives: </strong>This study aimed to evaluate the filling quality of simulated apical deltas in 3D-printed tooth replicas using microcomputed tomography and comparisons of two obturation techniques and calcium silicate cement-based sealer groups.</p><p><strong>Methodology: </strong>A single-rooted, straight maxillary premolar was instrumented and scanned by microcomputed tomography to generate a 3D model. Apical delta configurations were digitally created and integrated into the canal anatomy. In total, 40 translucent resin replicas were 3D printed and randomly assigned for obturation with one of four sealer groups (AH Plus Resin, AH Plus Bioceramic, Bio-C Sealer, and NeoSealer Flo). Following obturation, all specimens were rescanned, and a volumetric analysis was performed to determine the percentage of the filled volume in the apical delta region. Statistical analyses included one-way ANOVA and the Kruskal-Wallis test (α=0.05).</p><p><strong>Results: </strong>The continuous wave technique resulted in significantly greater apical delta filling than the single-cone one regardless of sealer. For the single-cone technique, NeoSealer Flo showed the highest filling percentage (42.7±2.0), followed by Bio-C Sealer (28.7±1.1), AH Plus Resin (24.9±1.9), and AH Plus Bioceramic (17.9±1.0). For the continuous wave technique, Bio-C Sealer showed the most filling (66.2±2.0), followed by NeoSealer Flo (54.2±1.4), AH Plus Resin (45.9±1.5) and AH Plus Bioceramic (36.8±1.0).</p><p><strong>Conclusions: </strong>The continuous wave technique achieved the most apical filling, with Bio-C sealer showing the highest performance. Bio-C and NeoSealer Flo achieved significantly higher filling percentages than AH Plus Resin and AH Plus Bioceramic under the single-cone technique.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"34 ","pages":"e20250774"},"PeriodicalIF":3.0,"publicationDate":"2026-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13123775/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147512357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Parkinson's disease and Porphyromonas gingivalis levels: a case-control study.","authors":"Nathália Alves Gusmão, Augusto Paixão Moraes Mateus, Victor Bento Oliveira, Luís Otávio de Miranda Cota, Rafael Paschoal Esteves Lima, José Roberto Cortelli, Sheila Cavalca Cortelli, Fernando Oliveira Costa","doi":"10.1590/1678-7765-2025-0780","DOIUrl":"10.1590/1678-7765-2025-0780","url":null,"abstract":"<p><strong>Objectives: </strong>This study aimed to evaluate the clinical and epidemiological aspects of the association between Parkinson's disease (PKD) and periodontitis (PE), including the quantification of Porphyromonas gingivalis and the influence of different risk variables on this association.</p><p><strong>Methodology: </strong>This case-control study included 172 individuals: 86 cases (PKD) and 86 controls (without any neurodegenerative disease). Participants underwent a complete periodontal examination (plaque index and probing depth), and clinical subgingival sampling was conducted to quantify Porphyromonas gingivalis counts. The association between PKD and PE was evaluated through univariate and binary logistic regression analyses.</p><p><strong>Results: </strong>A high prevalence of PE (73.2%) was observed among individuals with PKD [OR=3.99 (2.00-7.59); p<0.001]. Variables that were significantly retained in the final binary logistic regression model for the occurrence of PKD were: PE (OR=3.23; p=0.003), mean P. gingivalis counts (OR= 3.39; p=0.036), lower number of teeth (OR= 2.21, p<0.001), low frequency of toothbrushing/day (OR=1.95; p=0.006), and bleeding on probing (OR=2.45; p<0.001). The significant positive correlations observed between P. gingivalis counts and clinical periodontal parameters were considered moderate to strong in individuals with PKD.</p><p><strong>Conclusions: </strong>A strong association between PKD and PE was demonstrated, with risk increasing in parallel with the severity of periodontal involvement. Furthermore, individuals with PKD exhibited significantly higher levels of P. gingivalis compared to controls.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"34 ","pages":"e20250780"},"PeriodicalIF":3.0,"publicationDate":"2026-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13123765/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147512286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical and aesthetic outcomes of enamel matrix derivative and subepithelial connective tissue graft using the modified coronally advanced tunnel technique: a randomized controlled trial.","authors":"Fatma Altiparmak, Fatma Ucan Yarkac","doi":"10.1590/1678-7765-2025-0775","DOIUrl":"10.1590/1678-7765-2025-0775","url":null,"abstract":"<p><strong>Objectives: </strong>This randomized controlled clinical trial evaluated the short-term clinical outcomes of the modified coronally advanced tunnel technique combined with three different regenerative approaches-subepithelial connective tissue graft (as the gold standard), enamel matrix derivative (as a donor-site-sparing alternative), and their combination (to explore potential synergistic effects)-in treating recession type 1 gingival defects.</p><p><strong>Methodology: </strong>A total of 52 systemically healthy patients (aged 18-60) with 90 recession type 1 gingival defects were enrolled and randomly assigned to one of the three treatment groups: Group 1 (n=18; subepithelial connective tissue graft), Group 2 (n=19; enamel matrix derivative), and Group 3 (n=15; both materials). Primary outcome consisted of the percentage of root coverage at 6 months. Secondary outcomes included plaque index, gingival index, gingival recession depth, gingival recession width, clinical attachment level, keratinized tissue width, gingival thickness, wound healing index, probing depth, and root coverage aesthetic score. Clinical parameters were measured at baseline, 3, and 6 months, and aesthetic outcomes were assessed at 6 months.</p><p><strong>Results: </strong>All groups showed significant improvements in clinical parameters. Mean root coverage was 80.4% for Group 1, 76.2% for Group 2, and 73.6% for Group 3, with no significant differences among them. Keratinized tissue width significantly increased in Group 1 and Group 3, but not in Group 2. Mean root coverage aesthetic score values were 7.74 ± 2.35, 7.54 ± 2.39, and 7.62 ± 1.97, respectively (p > 0.05).</p><p><strong>Conclusion: </strong>Within the limitations of this study, including its short-term follow-up period and baseline differences in recession width, all three groups exhibited comparable clinical and aesthetic outcomes at 6 months. Clinical Trial Registration Number: NCT06504329.</p>","PeriodicalId":15133,"journal":{"name":"Journal of Applied Oral Science","volume":"34 ","pages":"e20250775"},"PeriodicalIF":3.0,"publicationDate":"2026-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13123777/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147512306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}