Journal of Biomedical Research最新文献_第6页

DEC1 deficiency protects against bone loss induced by ovariectomy through inhibiting inflammation. DEC1 缺乏症可通过抑制炎症防止卵巢切除术引起的骨质流失。

IF 2.2 4区医学

Journal of Biomedical Research Pub Date : 2024-05-25 DOI: 10.7555/JBR.38.20240069

Lan Lin, Zhiyi Qiang, Kaiao Chen, Ying Huo, Wei Liu, Jian Yang

{"title":"DEC1 deficiency protects against bone loss induced by ovariectomy through inhibiting inflammation.","authors":"Lan Lin, Zhiyi Qiang, Kaiao Chen, Ying Huo, Wei Liu, Jian Yang","doi":"10.7555/JBR.38.20240069","DOIUrl":"10.7555/JBR.38.20240069","url":null,"abstract":"Previous studies have shown that differentiated embryo-chondrocyte expressed gene 1 (DEC1) promotes osteoblast osteogenesis. To investigate the role of DEC1 in postmenopausal osteoporosis (PMOP), we utilized the two types (DEC1 +/+, DEC1 -/-) mice to establish an ovariectomy (OVX) model and found that the bone loss in DEC1 -/- OVX mice were much less than that in DEC1 +/+ OVX mice. The expression levels of RUNX2 and OSX significantly increased in DEC1 -/- OVX mice compared with those in DEC1 +/+ OVX mice. Whereas, NFATc1, c-Fos, CTSK and RANKL/OPG significantly decreased in DEC1 -/- OVX mice compared with those in DEC1 +/+ OVX mice. Likewise, DEC1 deficiency suppressed IL-6 and IL-1β. Further study showed Runx2, Osx, Alp, and Ocn significantly increased in DEC1 -/- OVX BMSCs compared with those in DEC1 +/+ OVX BMSCs. And the mRNA levels of IL-1β, IL-6, Tnf-α and Ifn-γ increased significantly in DEC1 +/+ OVX BMMs compared with those in DEC1 +/+ sham BMMs, but not in DEC1 -/- OVX BMMs compared with those in DEC1 -/- sham BMMs. Furthermore, the p-IκBα and p-P65 significantly increased in DEC1 +/+ OVX BMMs compared with those in DEC1 +/+ sham BMMs, but did not increase in DEC1 -/- OVX BMMs compared with those in DEC1 -/- sham BMMs. Taken together, DEC1 deficiency inhibits the NF-κB pathway induced by OVX, thereby decreasing cytokines, and subsequently, inhibits the decrease of osteogenesis and the increase of osteoclastogenesis caused by OVX. The findings provide a novel understanding of postmenopausal osteoporosis development, which offers potential avenues for the intervention strategies.","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":" ","pages":"1-16"},"PeriodicalIF":2.2,"publicationDate":"2024-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11629160/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141161374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Post-transcriptional dysregulation in autism, schizophrenia, and bipolar disorder. 自闭症、精神分裂症和躁狂症的转录后失调。

IF 2.3 4区医学

Journal of Biomedical Research Pub Date : 2024-05-25 DOI: 10.7555/JBR.38.20240114

Yuanyuan Wang, Yitong Yan, Bin Zhou, Mingyan Lin

{"title":"Post-transcriptional dysregulation in autism, schizophrenia, and bipolar disorder.","authors":"Yuanyuan Wang, Yitong Yan, Bin Zhou, Mingyan Lin","doi":"10.7555/JBR.38.20240114","DOIUrl":"https://doi.org/10.7555/JBR.38.20240114","url":null,"abstract":"The alteration of gene expression is not restricted to transcriptional regulation but includes a variety of post-transcriptional mechanisms, however, the role of the latter underlying many diseases remains relatively unknown. By utilizing an RNA-Seq dataset of 1510 brain samples from individuals with autism spectrum disorder (ASD), bipolar disorder (BD), schizophrenia (SCZ), and controls, we assessed the contribution of post-transcriptional dysregulation and identified top perturbators accountable for transcriptomic changes of expression in neuropsychiatric disorders. Around 30% of the variability in expression can be attributed to post-transcriptional dysregulation. Interestingly, RNA stability tended to decrease in SCZ and BD, leading to the inhibition of neurogenesis and neural differentiation, while the increase in ASD, resulted in enhanced activity of apoptosis. This finding implicated contrasting pathologies involving RNA stability among neuropsychiatric disorders. An RNA binding protein (RBP)-ELAVL3 - is predicted to be significantly involved in the disruption of RNA stability in all three disorders. To validate, we knocked down its expression in cerebral organoids. Not only differentially expressed genes in ELAVL3-knockdown covered a considerable proportion of predicted targets in three disorders, we also found neurogenesis was significantly affected, given the diminished proliferation and consequently the reduced size of the organoids. Our study extends the current understanding of the link between post-transcriptional regulation and neuropsychiatric disorders and provides new therapeutic targets for early intervention.","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":" ","pages":"1-14"},"PeriodicalIF":2.3,"publicationDate":"2024-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141161684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of SRPK1-mediated phosphorylation of SR proteins in the chromatin configuration transition of mouse germinal vesicle oocytes. SRPK1 介导的 SR 蛋白磷酸化在小鼠生殖泡卵母细胞染色质构型转变中的作用

IF 2.3 4区医学

Journal of Biomedical Research Pub Date : 2024-05-25 DOI: 10.7555/JBR.38.20240054

Xia Wang, Shuai Zhou, Haojie Yin, Jian Han, Yue Hu, Siqi Wang, Congjing Wang, Jie Huang, Junqiang Zhang, Xiufeng Ling, Ran Huo

{"title":"The role of SRPK1-mediated phosphorylation of SR proteins in the chromatin configuration transition of mouse germinal vesicle oocytes.","authors":"Xia Wang, Shuai Zhou, Haojie Yin, Jian Han, Yue Hu, Siqi Wang, Congjing Wang, Jie Huang, Junqiang Zhang, Xiufeng Ling, Ran Huo","doi":"10.7555/JBR.38.20240054","DOIUrl":"https://doi.org/10.7555/JBR.38.20240054","url":null,"abstract":"Meiotic resumption in mammalian oocytes involves nucleus and organelle structural changes, notably chromatin configuration transitioning from non-surrounding nucleolus (NSN) to surrounding nucleolus (SN) in germinal vesicle (GV) oocytes. Our study found that nuclear speckles, a subnuclear structure mainly composed of serine-arginine (SR) proteins, changed from a diffuse spotted distribution in mouse NSN oocytes to an aggregation pattern in SN oocytes. We further discovered that SRPK1, an enzyme phosphorylating SR proteins, co-localized with NS at SN stage and NSN oocytes failed to convert into SN oocytes after inhibiting the activity of SRPK1. Furthermore, the typical structure of chromatin ring around the nucleolus in SN oocytes collapsed after inhibitor treatment. To explore the underlying mechanism, phosphorylated SR proteins were confirmed to be associated with chromatin by salt extraction experiment, and in situ DNase I assay showed that the accessibility of chromatin enhanced in SN oocytes with SRPK1 inhibited, accompanied by decreased repressive modification on histone and abnormal recurrence of transcriptional signal. In conclusion, our results indicated that SRPK1-regulated phosphorylation on SR proteins was involved in the NSN to SN transition and played an important role in maintaining the condensation nucleus of SN oocytes via interacting with chromatin.","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":" ","pages":"1-11"},"PeriodicalIF":2.3,"publicationDate":"2024-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141161696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Atherosclerosis originating from childhood: Specific features. 源自童年的动脉粥样硬化：具体特征。

IF 2.3 4区医学

Journal of Biomedical Research Pub Date : 2024-05-22 DOI: 10.7555/JBR.37.20230198

Anastasia V Poznyak, Alexey A Yakovlev, Mikhail А Popov, Elena B Zhigmitova, Vasily N Sukhorukov, Alexander N Orekhov

引用次数: 0

RNA-sequencing expression profile and functional analysis of retinal pigment epithelium in atrophic age-related macular degeneration. 萎缩性老年性黄斑变性患者视网膜色素上皮细胞的 RNA 序列表达谱和功能分析。

IF 2.2 4区医学

Journal of Biomedical Research Pub Date : 2024-04-25 DOI: 10.7555/JBR.37.20230320

Miao Xu, Yan Gao, Wenjie Yin, Qinghuai Liu, Songtao Yuan

{"title":"RNA-sequencing expression profile and functional analysis of retinal pigment epithelium in atrophic age-related macular degeneration.","authors":"Miao Xu, Yan Gao, Wenjie Yin, Qinghuai Liu, Songtao Yuan","doi":"10.7555/JBR.37.20230320","DOIUrl":"10.7555/JBR.37.20230320","url":null,"abstract":"The retinal pigment epithelium (RPE) is fundamental to sustaining retinal homeostasis. RPE abnormality leads to visual defects and blindness, including age-related macular degeneration (AMD). Although breakthroughs have been made in the treatment of neovascular AMD, effective intervention for atrophic AMD is largely absent. The inadequate knowledge of RPE pathology is hindered by a lack of patient RPE datasets, especially at the single-cell resolution. In this study, we delved into a large-scale single-cell resource of AMD donors in which RPE cells were occupied in a substantial proportion. Bulk RNA-seq datasets of atrophic AMD were integrated to extract molecular characteristics of RPE in the pathogenesis of atrophic AMD. Both in vivo and in vitro models revealed that carboxypeptidase X, M14 family member 2 (CPXM2) was specifically expressed in the RPE cells of atrophic AMD, which might be induced by oxidative stress and involved in the epithelial-mesenchymal transition of RPE cells. Additionally, silencing of CPXM2 inhibited the mesenchymal phenotype of RPE cells in an oxidative stress cell model. Thus, our results demonstrate that CPXM2 plays a crucial role in regulating atrophic AMD and may serve as a potential therapeutic target for atrophic AMD.","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":" ","pages":"1-12"},"PeriodicalIF":2.2,"publicationDate":"2024-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11461538/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141161688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of pericyte in ocular vascular diseases. 眼周膜在眼部血管疾病中的作用。

IF 2.2 4区医学

Journal of Biomedical Research Pub Date : 2024-04-25 DOI: 10.7555/JBR.37.20230314

Lian Jun Shi, HuiMin Ge, Fan Ye, Xiumiao Li, Qin Jiang

{"title":"The role of pericyte in ocular vascular diseases.","authors":"Lian Jun Shi, HuiMin Ge, Fan Ye, Xiumiao Li, Qin Jiang","doi":"10.7555/JBR.37.20230314","DOIUrl":"10.7555/JBR.37.20230314","url":null,"abstract":"Pericytes are located in the stromal membrane of the capillary outer wall and contain endothelial cells (ECs). They are pivotal in regulating blood flow, enhancing vascular stability, and maintaining the integrity of the blood-retina barrier (BRB)/blood-brain barrier (BBB). The pluripotency of pericytes allows them to differentiate into various cell types, highlighting their significance in vascular disease pathogenesis, as demonstrated by previous studies. This potential enables pericytes to be a potential biomarker for the diagnosis and a target for treatment of vascular disorders. The retina, an essential part of the eyeball, is an extension of cerebral tissue with a transparent refractive medium. It offers a unique window for assessing systemic microvascular lesions. Routine fundus examination is necessary for patients with diabetes and hypertension. Manifestations, such as retinal artery tortuosity, dilation, stenosis, and abnormal arteriovenous anastomosis, serve as typical hallmarks of retinal vasculopathy. Therefore, studies of ocular vascular diseases significantly facilitate the exploration of systemic vascular diseases.","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":" ","pages":"1-10"},"PeriodicalIF":2.2,"publicationDate":"2024-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11629158/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141161691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mouse KL2 is a unique MTSE involved in chromosome-based spindle organization and regulated by multiple kinases during female meiosis. 小鼠 KL2 是一种独特的 MTSE，参与基于染色体的纺锤体组织，在雌性减数分裂过程中受多种激酶调控。

IF 2.2 4区医学

Journal of Biomedical Research Pub Date : 2024-04-25 DOI: 10.7555/JBR.37.20230290

Shi-Ya Xie, Yan-Jie Yang, Zhen Jin, Xiao-Cong Liu, Shu-Ping Zhang, Ning Su, Jia-Qi Liu, Cong-Rong Li, Dong Zhang, Lei-Lei Gao, Zhi-Xia Yang

{"title":"Mouse KL2 is a unique MTSE involved in chromosome-based spindle organization and regulated by multiple kinases during female meiosis.","authors":"Shi-Ya Xie, Yan-Jie Yang, Zhen Jin, Xiao-Cong Liu, Shu-Ping Zhang, Ning Su, Jia-Qi Liu, Cong-Rong Li, Dong Zhang, Lei-Lei Gao, Zhi-Xia Yang","doi":"10.7555/JBR.37.20230290","DOIUrl":"10.7555/JBR.37.20230290","url":null,"abstract":"Microtubule-severing enzymes (MTSEs) play important roles in mitosis and meiosis of the primitive organisms. However, no studies have assessed their roles in mammalian meiosis of females, whose abnormality accounts for over 80% of the cases of gamete-originated human reproductive disease. In the current study, we reported that katanin-like 2 (KL2) was the only MTSE concentrating at chromosomes. Furthermore, the knockdown of KL2 significantly reduced chromosome-based increase in the microtubule (MT) polymer, increased aberrant kinetochore-MT (K-MT) attachment, delayed meiosis, and severely affected normal fertility. Importantly, we demonstrated that the inhibition of aurora B, a key kinase for correcting aberrant K-MT attachment, eliminated KL2 from chromosomes completely. KL2 also interacted with phosphorylated eukaryotic elongation factor-2 kinase; they competed for chromosome binding. We also observed that the phosphorylated KL2 was localized at spindle poles, and that KL2 phosphorylation was regulated by extracellular signal-regulated kinase 1/2. In summary, our study reveals a novel function of MTSEs in mammalian female meiosis and demonstrates that multiple kinases coordinate to regulate the levels of KL2 at chromosomes.","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":" ","pages":"1-15"},"PeriodicalIF":2.2,"publicationDate":"2024-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11461529/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141161645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Coronary atherosclerotic plaque regression strategies. 冠状动脉粥样硬化斑块消退策略。

IF 2.3 4区医学

Journal of Biomedical Research Pub Date : 2024-04-25 DOI: 10.7555/JBR.37.20230223

Anastasia V Poznyak, Alexey Aleksandrovich Yakovlev, Mikhail А Popov, Alexander D Zhuravlev, Vasily N Sukhorukov, Alexander N Orekhov

引用次数: 0

Ferroptosis: Iron-mediated cell death linked to disease pathogenesis. 铁变态反应：铁介导的细胞死亡与疾病发病机制有关。

IF 2.2 4区医学

Journal of Biomedical Research Pub Date : 2024-03-25 DOI: 10.7555/JBR.37.20230224

Xiangyu Zhang, Yingchao Hu, Bingwei Wang, Shuo Yang

引用次数: 0

Evaluating short-term and long-term liver fibrosis improvement in hepatitis C patients post-DAA treatment. 评估 DAA 治疗后丙型肝炎患者肝纤维化的短期和长期改善情况。

IF 2.2 4区医学

Journal of Biomedical Research Pub Date : 2024-03-21 DOI: 10.7555/JBR.37.20230284

Yifan Wang, Xinyan Ma, Yanzheng Zou, Ming Yue, Meiling Zhang, Rongbin Yu, Hongbo Chen, Peng Huang

{"title":"Evaluating short-term and long-term liver fibrosis improvement in hepatitis C patients post-DAA treatment.","authors":"Yifan Wang, Xinyan Ma, Yanzheng Zou, Ming Yue, Meiling Zhang, Rongbin Yu, Hongbo Chen, Peng Huang","doi":"10.7555/JBR.37.20230284","DOIUrl":"10.7555/JBR.37.20230284","url":null,"abstract":"Despite achieving a high cure rate with direct-acting antivirals (DAAs) in hepatitis C treatment, further research is needed to identify additional benefits of the DAA therapy. The current study evaluated liver fibrosis improvement in 848 hepatitis C patients treated with DAAs, who also achieved sustained virologic response (SVR). Using the fibrosis-4 (FIB-4) index, patients were categorized based on their baseline fibrosis level, and improvements in fibrosis were analyzed in both the short-term (9-26 weeks) and long-term (≥ 36 weeks) follow-up. The results showed a significant decrease in the FIB-4 index, indicating an improvement in liver fibrosis, in 63.00% of the patients during the short-term follow-up and 67.56% during the long-term follow-up. Short-term improvement was associated with factors including ribavirin (RBV) usage, blood cholinesterase levels, alanine transaminase levels, albumin levels, and the baseline FIB-4 index. Additionally, long-term improvement was associated with factors such as aspartate transaminase levels, total protein level, and the baseline FIB-4 index. The current study emphasizes the importance of continuous assessment and post-treatment monitoring of liver fibrosis, providing crucial insights for enhancing patient care in hepatitis C management.","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":" ","pages":"1-10"},"PeriodicalIF":2.2,"publicationDate":"2024-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11461531/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141161455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0