Journal of Biomedical Research最新文献_第7页

Expression profiling and bioinformatics analysis of serum exosomal circular RNAs in lymph node metastasis of papillary thyroid carcinoma. 甲状腺乳头状癌淋巴结转移中血清外泌体环状RNA的表达谱分析和生物信息学分析

IF 2.2 4区医学

Journal of Biomedical Research Pub Date : 2024-05-30 DOI: 10.7555/JBR.37.20230304

Huiyong Peng, Zhangwei Zhu, Jie Xing, Qian Xu, Changfeng Man, Shengjun Wang, Yingzhao Liu, Zhengdong Zhang

{"title":"Expression profiling and bioinformatics analysis of serum exosomal circular RNAs in lymph node metastasis of papillary thyroid carcinoma.","authors":"Huiyong Peng, Zhangwei Zhu, Jie Xing, Qian Xu, Changfeng Man, Shengjun Wang, Yingzhao Liu, Zhengdong Zhang","doi":"10.7555/JBR.37.20230304","DOIUrl":"10.7555/JBR.37.20230304","url":null,"abstract":"Most papillary thyroid carcinoma (PTC) patients have a good prognosis. However, lymph node metastasis (LNM), the most common manifestation of disease progression, is frequently associated with a poor prognosis. Nevertheless, few studies have focused on the underlying mechanisms of LNM. In the current study, we aimed to investigate the potential role of exosomal circRNAs that contribute to LNM in PTC. We identified 9000 differentially expressed exosomal circRNAs in PTC patients with LNM, including 684 upregulated and 2193 downregulated circRNAs. Functional enrichment analysis revealed that these differentially expressed circRNAs were primarily involved in a variety of molecular and signaling pathways correlated with PTC progression and LNM. Through bioinformatics analysis, we identified 14 circRNA-miRNA-mRNA networks related to LNM-associated signaling pathways in PTC. Moreover, both circTACC2-miR-7- EGFR and circBIRC6-miR-24-3p- BCL2L11 axes were verified for their potential involvement in PTC with LNM. Additionally, we identified four upregulated circRNA-related hub genes and eight hub genes correlated with downregulated circRNAs, some of which were validated as being potentially involved in LNM in PTC. Collectively, our findings provide a novel framework for an in-depth investigation of the function of dysregulated exosomal circRNAs and their potential as biomarkers in PTC patients with LNM.","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":" ","pages":"155-170"},"PeriodicalIF":2.2,"publicationDate":"2024-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11982685/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141175361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of cell surface markers for acute myeloid leukemia prognosis based on multi-model analysis. 基于多模型分析鉴定急性髓性白血病预后的细胞表面标志物

IF 2.2 4区医学

Journal of Biomedical Research Pub Date : 2024-05-29 DOI: 10.7555/JBR.38.20240065

Jiaqi Tang, Lin Luo, Bakwatanisa Bosco, Ning Li, Bin Huang, Rongrong Wu, Zihan Lin, Ming Hong, Wenjie Liu, Lingxiang Wu, Wei Wu, Mengyan Zhu, Quanzhong Liu, Peng Xia, Miao Yu, Diru Yao, Sali Lv, Ruohan Zhang, Wentao Liu, Qianghu Wang, Kening Li

{"title":"Identification of cell surface markers for acute myeloid leukemia prognosis based on multi-model analysis.","authors":"Jiaqi Tang, Lin Luo, Bakwatanisa Bosco, Ning Li, Bin Huang, Rongrong Wu, Zihan Lin, Ming Hong, Wenjie Liu, Lingxiang Wu, Wei Wu, Mengyan Zhu, Quanzhong Liu, Peng Xia, Miao Yu, Diru Yao, Sali Lv, Ruohan Zhang, Wentao Liu, Qianghu Wang, Kening Li","doi":"10.7555/JBR.38.20240065","DOIUrl":"10.7555/JBR.38.20240065","url":null,"abstract":"Given the extremely high inter-patient heterogeneity of acute myeloid leukemia (AML), the identification of biomarkers for prognostic assessment and therapeutic guidance is critical. Cell surface markers (CSMs) have been shown to play an important role in AML leukemogenesis and progression. In the current study, we evaluated the prognostic potential of all human CSMs in 130 AML patients from The Cancer Genome Atlas (TCGA) based on differential gene expression analysis and univariable Cox proportional hazards regression analysis. By using multi-model analysis, including Adaptive LASSO regression, LASSO regression, and Elastic Net, we constructed a 9-CSMs prognostic model for risk stratification of the AML patients. The predictive value of the 9-CSMs risk score was further validated at the transcriptome and proteome levels. Multivariable Cox regression analysis showed that the risk score was an independent prognostic factor for the AML patients. The AML patients with high 9-CSMs risk scores had a shorter overall and event-free survival time than those with low scores. Notably, single-cell RNA-sequencing analysis indicated that patients with high 9-CSMs risk scores exhibited chemotherapy resistance. Furthermore, PI3K inhibitors were identified as potential treatments for these high-risk patients. In conclusion, we constructed a 9-CSMs prognostic model that served as an independent prognostic factor for the survival of AML patients and held the potential for guiding drug therapy.","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":" ","pages":"397-412"},"PeriodicalIF":2.2,"publicationDate":"2024-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11300515/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141161598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

hUCMSC-derived extracellular vesicles relieve cisplatin-induced granulosa cell apoptosis in mice by transferring anti-apoptotic miRNAs. 源自 hUCMSC 的细胞外囊泡通过转移抗凋亡 miRNA 缓解顺铂诱导的小鼠颗粒细胞凋亡。

IF 2.2 4区医学

Journal of Biomedical Research Pub Date : 2024-05-29 DOI: 10.7555/JBR.37.20230310

Wenjing Tang, Haiyan Yan, Xiaojun Chen, Yanan Pu, Xin Qi, Liyang Dong, Chuan Su

{"title":"hUCMSC-derived extracellular vesicles relieve cisplatin-induced granulosa cell apoptosis in mice by transferring anti-apoptotic miRNAs.","authors":"Wenjing Tang, Haiyan Yan, Xiaojun Chen, Yanan Pu, Xin Qi, Liyang Dong, Chuan Su","doi":"10.7555/JBR.37.20230310","DOIUrl":"10.7555/JBR.37.20230310","url":null,"abstract":"Premature ovarian insufficiency (POI) caused by chemotherapy is a common complication in female cancer survivors of childbearing age. Traditional methods, including mesenchymal stem cell (MSC) transplant and hormone replacement therapy, have limited clinical application because of their drawbacks, and more methods need to be developed. In the current study, the potential effects and underlying mechanisms of human umbilical cord MSC-derived extracellular vesicles (hUCMSC-EVs) were investigated in a cisplatin (CDDP)-induced POI mouse model and a human granulosa cell (GC) line. The results showed that hUCMSC-EVs significantly attenuated body weight loss, ovarian weight loss, ovary atrophy, and follicle loss in moderate-dose (1.5 mg/kg) CDDP-induced POI mice, similar to the effects observed with hUCMSCs. We further found that the hUCMSC-EVs inhibited CDDP-induced ovarian GC apoptosis by upregulating anti-apoptotic miRNA levels in GCs, thereby downregulating the mRNA levels of multiple pro-apoptotic genes. In general, our findings indicate that the moderate-dose chemotherapy may be a better choice for clinical oncotherapy, considering effective rescue of the oncotherapy-induced ovarian damage with hUCMSC-EVs. Additionally, multiple miRNAs in hUCMSC-EVs may potentially be used to inhibit the chemotherapy-induced ovarian GC apoptosis, thereby restoring ovarian function and improving the life quality of female cancer patients.","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":" ","pages":"36-49"},"PeriodicalIF":2.2,"publicationDate":"2024-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11873591/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141161544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of common genetic variants in KCNQ family genes associated with gastric cancer survival in a Chinese population. 鉴定中国人群中与胃癌存活率相关的 KCNQ 家族基因的常见遗传变异。

IF 2.2 4区医学

Journal of Biomedical Research Pub Date : 2024-05-29 DOI: 10.7555/JBR.38.20240040

Yuetong Chen, Chen Li, Yi Shi, Jiali Dai, Yixuan Meng, Shuwei Li, Cuiju Tang, Dongying Gu, Jinfei Chen

{"title":"Identification of common genetic variants in KCNQ family genes associated with gastric cancer survival in a Chinese population.","authors":"Yuetong Chen, Chen Li, Yi Shi, Jiali Dai, Yixuan Meng, Shuwei Li, Cuiju Tang, Dongying Gu, Jinfei Chen","doi":"10.7555/JBR.38.20240040","DOIUrl":"10.7555/JBR.38.20240040","url":null,"abstract":"The KCNQ family of genes ( KCNQ1- KCNQ5), encoding voltage-gated K + (Kv) channels, have been demonstrated to play potential pathophysiological roles in cancers. However, the associations between genetic variants located in KCNQ family genes and gastric cancer survival remain unclear. In this study, a large-scale cohort comprising 1135 Chinese gastric cancer patients was enrolled to identify genetic variants in KCNQ family genes associated with overall survival (OS). Based on the survival evaluation of all five KCNQ family genes, KCNQ1 was selected for subsequent genetic analysis. In both Cox regression model and stepwise Cox regression model used to evaluate survival-related genetic variants, we found that KCNQ1 rs10832417G>T was associated with an increased OS in gastric cancer patients (adjusted hazards ratio [HR] = 0.84, 95% confidence interval [CI]: 0.72-0.98, P = 0.023). Subsequently, a nomogram was constructed to enhance the prognostic capacity and clinical translation of rs10832417 variants. The rs10832417 T allele was predicted to increase the minimum free energy of the secondary structure. Furthermore, we observed that gastric cancer patients with downregulated KCNQ1 expression had a poorer survival across multiple public datasets. The findings of the present study indicate that KCNQ1 rs10832417 may serve as an independent prognostic predictor of gastric cancer, providing novel insights into the progression and survival of the disease.","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":" ","pages":"76-86"},"PeriodicalIF":2.2,"publicationDate":"2024-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11873595/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141161612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Medical ozone alleviates acute lung injury by enhancing phagocytosis targeting NETs via AMPK/SR-A1 axis. 医用臭氧通过 AMPK/SR-A1 轴增强针对 NET 的吞噬作用，从而缓解急性肺损伤。

IF 2.2 4区医学

Journal of Biomedical Research Pub Date : 2024-05-29 DOI: 10.7555/JBR.38.20240038

Chenxiao Yan, Yong Zhang, Lai Jin, Xiaojie Liu, Xuexian Zhu, Qifeng Li, Yu Wang, Liang Hu, Xueming He, Hongguang Bao, Xia Zhu, Qian Wang, Wen-Tao Liu

{"title":"Medical ozone alleviates acute lung injury by enhancing phagocytosis targeting NETs via AMPK/SR-A1 axis.","authors":"Chenxiao Yan, Yong Zhang, Lai Jin, Xiaojie Liu, Xuexian Zhu, Qifeng Li, Yu Wang, Liang Hu, Xueming He, Hongguang Bao, Xia Zhu, Qian Wang, Wen-Tao Liu","doi":"10.7555/JBR.38.20240038","DOIUrl":"10.7555/JBR.38.20240038","url":null,"abstract":"Acute lung injury (ALI) linked to sepsis has a high mortality rate, with limited treatment options available. In recent studies, medical ozone has shown the potential to alleviate inflammation and infection. Here, we aimed to evaluate therapeutic potential of medical ozone in a mouse model of the sepsis-induced ALI by measuring behavioral assessments, lung function, and blood flow. Protein levels were quantified by Western blotting. In vitro, we performed experiments on bone marrow-derived macrophages (BMDMs) to investigate the effect of adenosine monophosphate (AMP)-activated protein kinase (AMPK) inhibitors and agonists on their phagocytic activity. The results showed that medical ozone significantly improved the survival rate, ameliorated lung injury, and enhanced lung function and limb microcirculation in mice with ALI. Notably, medical ozone inhibited the formation of neutrophil extracellular traps (NETs), a crucial factor in the ALI development. Additionally, medical ozone counteracted the elevated levels of tissue factor, matrix metalloproteinase-9, and interleukin-1β. In the ALI mice, the effects of ozone were abolished, and BMDMs showed an impaired capacity to engulf NETs following the Sr-a1 knockout. Under normal physiological conditions, the administration of an AMPK antagonist showed similar effects on the Sr-a1 knockout, significantly inhibiting the phagocytosis of NETs by BMDMs. In contrast, AMPK agonists enhanced this phagocytic process. In conclusion, medical ozone may alleviate the sepsis-induced lung injury through the AMPK/SR-A1 pathway, thereby enhancing the phagocytosis of NETs by macrophages.","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":" ","pages":"569-584"},"PeriodicalIF":2.2,"publicationDate":"2024-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11629159/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141161630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RNA-sequencing expression profile and functional analysis of retinal pigment epithelium in atrophic age-related macular degeneration. 萎缩性老年性黄斑变性患者视网膜色素上皮细胞的 RNA 序列表达谱和功能分析。

IF 2.2 4区医学

Journal of Biomedical Research Pub Date : 2024-05-29 DOI: 10.7555/JBR.37.20230320

Miao Xu, Yan Gao, Wenjie Yin, Qinghuai Liu, Songtao Yuan

{"title":"RNA-sequencing expression profile and functional analysis of retinal pigment epithelium in atrophic age-related macular degeneration.","authors":"Miao Xu, Yan Gao, Wenjie Yin, Qinghuai Liu, Songtao Yuan","doi":"10.7555/JBR.37.20230320","DOIUrl":"10.7555/JBR.37.20230320","url":null,"abstract":"The retinal pigment epithelium (RPE) is fundamental to sustaining retinal homeostasis. RPE abnormality leads to visual defects and blindness, including age-related macular degeneration (AMD). Although breakthroughs have been made in the treatment of neovascular AMD, effective intervention for atrophic AMD is largely absent. The adequate knowledge of RPE pathology is hindered by a lack of the patients' RPE datasets, especially at the single-cell resolution. In the current study, we delved into a large-scale single-cell resource of AMD donors, in which RPE cells were occupied in a substantial proportion. Bulk RNA-seq datasets of atrophic AMD were integrated to extract molecular characteristics of RPE in the pathogenesis of atrophic AMD. Both in vivo and in vitro models revealed that carboxypeptidase X, M14 family member 2 (CPXM2), was specifically expressed in the RPE cells of atrophic AMD, which might be induced by oxidative stress and involved in the epithelial-mesenchymal transition of RPE cells. Additionally, silencing of CPXM2 inhibited the mesenchymal phenotype of RPE cells in an oxidative stress cell model. Thus, our results demonstrated that CPXM2 played a crucial role in regulating atrophic AMD and might serve as a potential therapeutic target for atrophic AMD.","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":" ","pages":"500-511"},"PeriodicalIF":2.2,"publicationDate":"2024-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11461538/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141161688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

First-episode psychiatric disorder risk from SARS-CoV-2 infection: A clinical analysis with Chinese psychiatric inpatients. 中国精神病住院患者感染 SARS-CoV-2 导致首发精神障碍的风险临床分析。

IF 2.2 4区医学

Journal of Biomedical Research Pub Date : 2024-05-29 DOI: 10.7555/JBR.38.20240005

Ya Xie, Zifeng Xu, Yumin Zhang, Yisheng Li, Pengyu Du, Chun Wang

{"title":"First-episode psychiatric disorder risk from SARS-CoV-2 infection: A clinical analysis with Chinese psychiatric inpatients.","authors":"Ya Xie, Zifeng Xu, Yumin Zhang, Yisheng Li, Pengyu Du, Chun Wang","doi":"10.7555/JBR.38.20240005","DOIUrl":"10.7555/JBR.38.20240005","url":null,"abstract":"The extensive spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) throughout China in late 2022 has underscored the correlation between this virus and severe psychiatric disorders. However, there remains a lack of reported clinical and pathological features. Accordingly, we retrospectively reviewed the electronic medical records of psychiatric inpatients for seven days from early January 2023. Twenty-one inpatients who developed first-episode psychiatric disorders within two weeks after SARS-CoV-2 infection were recruited, while 24 uninfected first-episode psychiatric inpatients were selected as controls. Comparative analyses of clinical manifestations, routine laboratory tests, and imaging examinations were performed. Our investigation demonstrated a 330% increase in the incidence of first-episode psychiatric inpatients after SARS-CoV-2 infection in 2023, compared with the preceding year without SARS-CoV-2 infections. Most cases exhibited psychiatric symptoms within one week of SARS-CoV-2 infection, which resolved after approximately two weeks, with no residual symptoms after three months. One-way ANOVA demonstrated a significant difference in the highest fever temperature between inpatients with and without psychotic symptoms. Infected inpatients displayed elevated levels of interleukin-4, interleukin-8, and interferon-α, but decreased levels of eosinophils and basophils. These findings suggest that SARS-CoV-2 may contribute to the development of psychiatric disorders, likely mediated by the virus-induced inflammatory response and neuronal dysfunction in the context of psychological distress.","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":" ","pages":"50-60"},"PeriodicalIF":2.2,"publicationDate":"2024-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11873594/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141161523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

p53 exerts anticancer effects by regulating enhancer formation and activity. p53 通过调节增强子的形成和活性发挥抗癌作用。

IF 2.2 4区医学

Journal of Biomedical Research Pub Date : 2024-05-29 DOI: 10.7555/JBR.37.20230206

Shuhan Chen, Xuchun Wang, Nan Yang, Yuechi Song, He Cheng, Yujie Sun

{"title":"p53 exerts anticancer effects by regulating enhancer formation and activity.","authors":"Shuhan Chen, Xuchun Wang, Nan Yang, Yuechi Song, He Cheng, Yujie Sun","doi":"10.7555/JBR.37.20230206","DOIUrl":"10.7555/JBR.37.20230206","url":null,"abstract":"The abnormality of the p53 tumor suppressor is crucial in lung cancer development, because p53 regulates target gene promoters to combat cancer. Recent studies have shown extensive p53 binding to enhancer elements. However, whether p53 exerts a tumor suppressor role by shaping the enhancer landscape remains poorly understood. In the current study, we employed several functional genomics approaches to assess the enhancer activity at p53 binding sites throughout the genome based on our established TP53 knockout (KO) human bronchial epithelial cells (BEAS-2B). A total of 943 active regular enhancers and 370 super-enhancers (SEs) disappeared upon the deletion of p53, indicating that p53 modulates the activity of hundreds of enhancer elements. We found that one p53-dependent SE, located on chromosome 9 and designated as KLF4-SE, regulated the expression of the Krüppel-like factor 4 ( KLF4) gene. Furthermore, the deletion of p53 significantly decreased the KLF4-SE enhancer activity and the KLF4 expression, but increased colony formation ability in the nitrosamines 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone-induced cell transformation model. Subsequently, in TP53 KO cells, the overexpression of KLF4 partially reversed the increased clonogenic capacity caused by p53 deficiency. Consistently, KLF4 expression also decreased in lung cancer tissues and cell lines. It appeared that overexpression of KLF4 significantly suppressed the proliferation and migration of lung cancer cells. Collectively, our results suggest that the regulation of enhancer formation and activity by p53 is an integral component of the p53 tumor suppressor function. Therefore, our findings offer some novel insights into the regulation mechanism of p53 in lung oncogenesis and introduce a new strategy for screening therapeutic targets.","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":" ","pages":"334-347"},"PeriodicalIF":2.2,"publicationDate":"2024-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11300520/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141161650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Human circBOULE RNAs as potential biomarkers for sperm quality and male infertility. 作为精子质量和男性不育症潜在生物标志物的人类 circBOULE RNAs。

IF 2.2 4区医学

Journal of Biomedical Research Pub Date : 2024-05-29 DOI: 10.7555/JBR.37.20230296

Liping Cheng, He Jin, Tianheng Xiao, Xiaoyu Yang, Tingting Zhao, Eugene Yujun Xu

{"title":"Human circBOULE RNAs as potential biomarkers for sperm quality and male infertility.","authors":"Liping Cheng, He Jin, Tianheng Xiao, Xiaoyu Yang, Tingting Zhao, Eugene Yujun Xu","doi":"10.7555/JBR.37.20230296","DOIUrl":"10.7555/JBR.37.20230296","url":null,"abstract":"Reliable molecular biomarkers to predict fertility remain scarce. The current study investigated the potential of testis-specific circBOULE RNAs as biomarkers for male infertility and sperm quality. Using reverse transcription-PCR and real-time reverse transcription-PCR assays, we identified seven circular RNAs from the human BOULE gene in human sperm. We observed that the expression level of circEx3-6 was significantly reduced in asthenozoospermia, while the expression levels of both circEx2-6 and circEx2-7were decreased in teratozoospermia, compared with the controls. Furthermore, we demonstrated that the expression level of circEx2-6 was negatively correlated with the sperm DNA fragmentation index, and the expression level of circEx2-7 was correlated with both fertilization and cleavage rates in those treated with the assisted reproductive technologies. Further functional analyses in a transgenic fly model supported the roles of circBOULE RNAs in sperm development and human male fertility. Collectively, our findings support that sperm circBOULE RNAs may serve as diagnostic biomarkers for assessing sperm motility and DNA quality. Therefore, clinical application and significance of sperm circBOULE RNAs in the assisted reproductive technologies warrant further investigation.","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":" ","pages":"473-484"},"PeriodicalIF":2.2,"publicationDate":"2024-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11461533/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141161550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

miR-19a-3p enhances TGF-β1-induced cardiac fibroblast activation via targeting BAMBI. MicroRNA-19a-3p 通过靶向 BAMBI 增强 TGF-β1 诱导的心脏成纤维细胞活化。

IF 2.2 4区医学

Journal of Biomedical Research Pub Date : 2024-05-29 DOI: 10.7555/JBR.37.20230313

Pengxi Shi, Ao Tan, Yuanyuan Ma, Lingli Que, Chuanfu Li, Yongfeng Shao, Haoliang Sun, Yuehua Li, Jiantao Li

{"title":"miR-19a-3p enhances TGF-β1-induced cardiac fibroblast activation via targeting BAMBI.","authors":"Pengxi Shi, Ao Tan, Yuanyuan Ma, Lingli Que, Chuanfu Li, Yongfeng Shao, Haoliang Sun, Yuehua Li, Jiantao Li","doi":"10.7555/JBR.37.20230313","DOIUrl":"10.7555/JBR.37.20230313","url":null,"abstract":"Myocardial fibrosis is a major pathogenic factor contributing to cardiac remodeling and heart failure. Recent research has indicated that microRNAs play a crucial role in the progression of cardiac fibrosis. Bone morphogenetic protein and activin membrane-bound inhibitor (BAMBI) have been shown to alleviate myocardial fibrosis by inhibiting the transforming growth factor β1 (TGF-β1) signaling pathway. Therefore, the current study aimed to elucidate the post-transcriptional regulation of BAMBI by miR-19a-3p and its role in TGF-β1-induced cardiac fibroblast activation. We found that transverse aortic constriction induced both myocardial interstitial and perivascular collagen deposition. Quantitative reverse transcription-PCR (qRT-PCR) analysis showed that the expression level of miR-19a-3p was increased in the myocardial tissues of cardiac fibrosis, and TGF-β1 induced an upregulation in miR-19a-3p expression in cardiac fibroblasts. The dual-luciferase reporter assay and qRT-PCR verified that miR-19a-3p directly bound to the 3' untranslated regions of BAMBI mRNA, thereby reducing BAMBI expression and diminishing its ability to inhibit the TGF-β1 signaling pathway. Furthermore, overexpression of miR-19a-3p mimic increased the activation of TGF-β1/SMAD2/3 pathway signaling, promoting cardiac fibroblast activation. However, this activation was blocked by BAMBI overexpression. These findings imply that miR-19a-3p enhances the activation of TGF-β1/SMAD2/3 by inhibiting BAMBI, further boosting the activation of cardiac fibroblasts and contributing to myocardial fibrosis.","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":" ","pages":"171-183"},"PeriodicalIF":2.2,"publicationDate":"2024-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11982684/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141161644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0