Journal of Biomedical Research最新文献

{"title":"Gut microbiota links with cognitive impairment in amyotrophic lateral sclerosis: A multi-omics study.","authors":"Zhenxiang Gong,&nbsp;Li Ba,&nbsp;Jiahui Tang,&nbsp;Yuan Yang,&nbsp;Zehui Li,&nbsp;Mao Liu,&nbsp;Chun Yang,&nbsp;Fengfei Ding,&nbsp;Min Zhang","doi":"10.7555/JBR.36.20220198","DOIUrl":"https://doi.org/10.7555/JBR.36.20220198","url":null,"abstract":"<p><p>Recently, cognitive impairments (CI) and behavioral abnormalities in patients with amyotrophic lateral sclerosis (ALS) have been reported. However, the underlying mechanisms have been poorly understood. In the current study, we explored the role of gut microbiota in CI of ALS patients. We collected fecal samples from 35 ALS patients and 35 healthy controls. The cognitive function of the ALS patients was evaluated using the Edinburgh Cognitive and Behavioral ALS Screen. We analyzed these samples by using 16S rRNA gene sequencing as well as both untargeted and targeted (bile acids) metabolite mapping between patients with CI and patients with normal cognition (CN). We found altered gut microbial communities and a lower ratio of <i>Firmicutes</i>/ <i>Bacteroidetes</i> in the CI group, compared with the CN group. In addition, the untargeted metabolite mapping revealed that 26 and 17 metabolites significantly increased and decreased, respectively, in the CI group, compared with the CN group. These metabolites were mapped to the metabolic pathways associated with bile acids. We further found that cholic acid and chenodeoxycholic acid were significantly lower in the CI group than in the CN group. In conclusion, we found that the gut microbiota and its metabolome profile differed between ALS patients with and without CI and that the altered bile acid profile in fecal samples was significantly associated with CI in ALS patients. These results need to be replicated in larger studies in the future.</p>","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":"37 2","pages":"125-137"},"PeriodicalIF":2.3,"publicationDate":"2022-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10018415/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9867884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A SARS-CoV-2 neutralizing antibody discovery by single cell sequencing and molecular modeling.","authors":"Zheyue Wang,&nbsp;Qi Tang,&nbsp;Bende Liu,&nbsp;Wenqing Zhang,&nbsp;Yufeng Chen,&nbsp;Ningfei Ji,&nbsp;Yan Peng,&nbsp;Xiaohui Yang,&nbsp;Daixun Cui,&nbsp;Weiyu Kong,&nbsp;Xiaojun Tang,&nbsp;Tingting Yang,&nbsp;Mingshun Zhang,&nbsp;Xinxia Chang,&nbsp;Jin Zhu,&nbsp;Mao Huang,&nbsp;Zhenqing Feng","doi":"10.7555/JBR.36.20220221","DOIUrl":"https://doi.org/10.7555/JBR.36.20220221","url":null,"abstract":"<p><p>Although vaccines have been developed, mutations of SARS-CoV-2, especially the dominant B.1.617.2 (delta) and B.1.529 (omicron) strains with more than 30 mutations on their spike protein, have caused a significant decline in prophylaxis, calling for the need for drug improvement. Antibodies are drugs preferentially used in infectious diseases and are easy to get from immunized organisms. The current study combined molecular modeling and single memory B cell sequencing to assess candidate sequences before experiments, providing a strategy for the fabrication of SARS-CoV-2 neutralizing antibodies. A total of 128 sequences were obtained after sequencing 196 memory B cells, and 42 sequences were left after merging extremely similar ones and discarding incomplete ones, followed by homology modeling of the antibody variable region. Thirteen candidate sequences were expressed, of which three were tested positive for receptor binding domain recognition but only one was confirmed as having broad neutralization against several SARS-CoV-2 variants. The current study successfully obtained a SARS-CoV-2 antibody with broad neutralizing abilities and provided a strategy for antibody development in emerging infectious diseases using single memory B cell BCR sequencing and computer assistance in antibody fabrication.</p>","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":"37 3","pages":"166-178"},"PeriodicalIF":2.3,"publicationDate":"2022-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10226085/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9536221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antitumor efficacy of multi-target <i>in situ</i> vaccinations with CpG oligodeoxynucleotides, anti-OX40, anti-PD1 antibodies, and aptamers.","authors":"Anastasia S Proskurina,&nbsp;Vera S Ruzanova,&nbsp;Genrikh S Ritter,&nbsp;Yaroslav R Efremov,&nbsp;Zakhar S Mustafin,&nbsp;Sergey A Lashin,&nbsp;Ekaterina A Burakova,&nbsp;Alesya A Fokina,&nbsp;Timofei S Zatsepin,&nbsp;Dmitry A Stetsenko,&nbsp;Olga Y Leplina,&nbsp;Alexandr A Ostanin,&nbsp;Elena R Chernykh,&nbsp;Sergey S Bogachev","doi":"10.7555/JBR.36.20220052","DOIUrl":"https://doi.org/10.7555/JBR.36.20220052","url":null,"abstract":"<p><p>To overcome immune tolerance to cancer, the immune system needs to be exposed to a multi-target action intervention. Here, we investigated the activating effect of CpG oligodeoxynucleotides (ODNs), mesyl phosphoramidate CpG ODNs, anti-OX40 antibodies, and OX40 RNA aptamers on major populations of immunocompetent cells <i>ex vivo</i>. Comparative analysis of the antitumor effects of <i>in situ</i> vaccination with CpG ODNs and anti-OX40 antibodies, as well as several other combinations, such as mesyl phosphoramidate CpG ODNs and OX40 RNA aptamers, was conducted. Antibodies against programmed death 1 (PD1) checkpoint inhibitors or their corresponding PD1 DNA aptamers were also added to vaccination regimens for analytical purposes. Four scenarios were considered: a weakly immunogenic Krebs-2 carcinoma grafted in CBA mice; a moderately immunogenic Lewis carcinoma grafted in C57Black/6 mice; and an immunogenic A20 B cell lymphoma or an Ehrlich carcinoma grafted in BALB/c mice. Adding anti-PD1 antibodies (CpG+αOX40+αPD1) to <i>in situ</i> vaccinations boosts the antitumor effect. When to be used instead of antibodies, aptamers also possess antitumor activity, although this effect was less pronounced. The strongest effect across all the tumors was observed in highly immunogenic A20 B cell lymphoma and Ehrlich carcinoma.</p>","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":"37 3","pages":"194-212"},"PeriodicalIF":2.3,"publicationDate":"2022-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10226083/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9544167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploration and preliminary clinical investigation of an adhesive approach for primary tooth restoration.","authors":"Xiangqin Xu,&nbsp;Jiansheng Zhu,&nbsp;May Lei Mei,&nbsp;Huaying Wu,&nbsp;Kaipeng Xie,&nbsp;Shoulin Wang,&nbsp;Yaming Chen","doi":"10.7555/JBR.36.20220188","DOIUrl":"https://doi.org/10.7555/JBR.36.20220188","url":null,"abstract":"<p><p>The current study aims to investigate a suitable adhesive for primary tooth enamel. Shear bond strength (SBS) of primary teeth and the length of resin protrusion were analyzed using one-way ANOVA with Bonferroni multiple comparison tests after etching with 35% H ₃PO ₄. SBS and marginal microleakage tests were conducted with Single Bond Universal (SBU)/Single Bond 2 (SB2) adhesives with or without pre-etching using a nonparametric Kruskal-Wallis test. Clinical investigations were performed to validate the adhesive for primary teeth restoration using Chi-square tests. Results showed that the SBS and length of resin protrusion increased significantly with the etching time. Teeth in the SBU with 35% H ₃PO ₄ pre-etching groups had higher bond strength and lower marginal microleakage than those in the SB2 groups. Mixed fractures were more common in the 35% H ₃PO ₄ etched 30 s + SB2/SBU groups. Clinical investigations showed significant differences between the two groups in cumulative retention rates at the 6-, 12- and 18-month follow-up evaluations, as well as in marginal adaptation, discoloration, and secondary caries at the 12- and 18-month follow-up assessments. Together, pre-etching primary teeth enamel for 30 s before SBU treatment improved clinical composite resin restoration, which can provide a suitable approach for restoration of primary teeth.</p>","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":"37 2","pages":"138-147"},"PeriodicalIF":2.3,"publicationDate":"2022-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10018413/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9515336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EphA3 deficiency in the hypothalamus promotes high-fat diet-induced obesity in mice.","authors":"Jubiao Zhang,&nbsp;Yang Chen,&nbsp;Lihong Yan,&nbsp;Xin Zhang,&nbsp;Xiaoyan Zheng,&nbsp;Junxia Qi,&nbsp;Fen Yang,&nbsp;Juxue Li","doi":"10.7555/JBR.36.20220168","DOIUrl":"https://doi.org/10.7555/JBR.36.20220168","url":null,"abstract":"<p><p>Erythropoietin-producing hepatocellular carcinoma A3 (EphA3) is a member of the largest subfamily of tyrosine kinase receptors-Eph receptors. Previous studies have shown that EphA3 is associated with tissue development. Recently, we have found that the expression of EphA3 is elevated in the hypothalamus of mice with diet-induced obesity (DIO). However, the role of EphA3 in hypothalamic-controlled energy metabolism remains unclear. In the current study, we demonstrated that the deletion of EphA3 in the hypothalamus by CRISPR/Cas9-mediated gene editing promotes obesity in male mice with high-fat diet feeding rather than those with normal chow diet feeding. Moreover, the deletion of hypothalamic EphA3 promotes high-fat DIO by increasing food intake and reducing energy expenditure. Knockdown of EphA3 leads to smaller intracellular vesicles in GT1-7 cells. The current study reveals that hypothalamic EphA3 plays important roles in promoting DIO.</p>","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":"37 3","pages":"179-193"},"PeriodicalIF":2.3,"publicationDate":"2022-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10226084/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9897915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Author Correction: A novel recombinant DNA vaccine encoding <i>Mycobacterium tuberculosis</i> ESAT-6 and FL protects against <i>Mycobacterium tuberculosis</i> challenge in mice.","authors":"Qingtao Jiang,&nbsp;Jing Zhang,&nbsp;Xia Chen,&nbsp;Mei Xia,&nbsp;Yanlai Lu,&nbsp;Wen Qiu,&nbsp;Ganzhu Feng,&nbsp;Dan Zhao,&nbsp;Yan Li,&nbsp;Fengxia He,&nbsp;Guangyong Peng,&nbsp;Yingwei Wang","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":"36 6","pages":"448-450"},"PeriodicalIF":2.3,"publicationDate":"2022-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9724156/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10541036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between soft drink consumption types and risk of lung cancer and all-cancer: A prospective study of PLCO data.","authors":"Dongfang You,&nbsp;Hongyang Xu,&nbsp;Xin Chen,&nbsp;Jiawei Zhou,&nbsp;Yaqian Wu,&nbsp;Yingdan Tang,&nbsp;Zhongtian Wang,&nbsp;Yang Zhao,&nbsp;Fang Shao","doi":"10.7555/JBR.36.20220135","DOIUrl":"https://doi.org/10.7555/JBR.36.20220135","url":null,"abstract":"<p><p>Diet/sugar-free soft drinks are considered to be healthier than regular soft drinks. However, few studies have examined the relationship between the types of soft drinks (regular and diet/sugar-free) and lung cancer (LC)/all-cancer (AC) risk. In this study, we comprehensively assessed the influence of the type of soft drink consumption on LC/AC risk based on the Prostate, Lung, Colorectal, and Ovarian (PLCO) Cancer Screening Trial. Multivariable Cox proportional hazards and competing risks Fine-Gray regression models adjusted for relevant confounders were used to estimate hazard ratios (HRs) and subdistribution HRs for different types of soft drink consumption. In the PLCO population, female subgroup, and the ever/current smoker subgroup, consumption of both regular and diet soft drinks was associated with a significantly reduced risk of LC compared with no soft drinks at all. For the non-lung cancer (NLC) risk, consumption of only diet soft drinks had a significant positive association for the total population and female subgroup. Based on our findings, it was suggested that partial replacement of regular soft drinks with diet soft drinks might be beneficial to LC prevention, especially for females and ever/current smokers. Additionally, completely replacing regular soft drinks with diet soft drinks might be detrimental to NLC prevention, especially for females.</p>","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":"36 6","pages":"390-400"},"PeriodicalIF":2.3,"publicationDate":"2022-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9724159/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10534512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"hucMSC-derived exosomes protect ovarian reserve and restore ovarian function in cisplatin treated mice.","authors":"Yue Xiao,&nbsp;Yue Peng,&nbsp;Chi Zhang,&nbsp;Wei Liu,&nbsp;Kehan Wang,&nbsp;Jing Li","doi":"10.7555/JBR.36.20220166","DOIUrl":"10.7555/JBR.36.20220166","url":null,"abstract":"<p><p>Anti-cancer therapy often causes premature ovarian insufficiency and infertility as the ovarian follicle reserve is extremely sensitive to chemotherapy drugs, such as cisplatin. Various fertility preservation methods have been explored for women, especially prepubertal girls undergoing radiotherapy and chemotherapy due to cancer. In recent years, mesenchymal stem cell-derived exosomes (MSC-exos) have been reported to play an important role in tissue repair and the treatment of various diseases. In the current study, we observed that human umbilical cord-derived MSC-exos (hucMSC-exos) after short-term culture improved follicular survival and development while receiving cisplatin treatment. Moreover, intravenous injection of hucMSC-exos improved ovarian function and ameliorated inflammatory environment within the ovary. The underlying mechanism of hucMSC-exos on fertility preservation was associated with the down-regulation of p53-related apoptosis and their anti-inflammatory function. Based on these findings, we propose that hucMSC-exos may be a potential approach to improve fertility in women diagnosed with cancer.</p>","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":" ","pages":"382-393"},"PeriodicalIF":2.3,"publicationDate":"2022-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10541778/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9481414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A pilot study of the relative number of circulating tumor cells and leukocytes containing actin-binding proteins in head and neck cancer patients.","authors":"Gelena Kakurina,&nbsp;Marina Stakheeva,&nbsp;Elena Sereda,&nbsp;Evgenia Sidenko,&nbsp;Olga Cheremisina,&nbsp;Evgeny Choinzonov,&nbsp;Irina Kondakova","doi":"10.7555/JBR.36.20220182","DOIUrl":"https://doi.org/10.7555/JBR.36.20220182","url":null,"abstract":"<p><p>Circulating tumor cells (CTCs) play an important role in tumor metastases, which is positively correlated with an increased risk of death. Actin-binding proteins, including cofilin (CFL1), profilin 1 (PFN1), and adenylate cyclase-associated protein 1 (CAP1), are thought to be involved in tumor cell motility and metastasis, specifically in head and neck squamous cell carcinoma (HNSCC). However, currently, there are no published studies on CFL1, PFN1, and CAP1 in CTCs and leukocytes in HNSCC patients. We assessed serum levels of CFL1, PFN1, and CAP1 and the number of CTCs and leukocytes containing these proteins in blood from 31 HNSCC patients (T1-4N0-2M0). The analysis used flow cytometry and an enzyme-linked immunosorbent assay kit. We found that CAP1 ⁺ CTCs and CAP1 ⁺ leukocyte subpopulations were prevalent in these HNSCC patient samples, while the prevalence rates of CFL1 ⁺ and PFN1 ⁺ CTCs were relatively low. Patients with stage T2-4N1-2M0 had CFL1 ⁺ and PFN1 ⁺ CTCs with an elevated PFN1 serum level, compared with the T1-3N0M0 group. In summary, the PFN1 serum level and the relative number of PFN1 ⁺CD326 ⁺ CTCs could be valuable prognostic markers for HNSCC metastases. The current study is the first to obtain data regarding the contents of actin-binding proteins (ABPs) in CTCs, and leukocytes in blood from HNSCC patients. This is also the first to assess the relationship between the number of CTCs subgroups and disease characteristics.</p>","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":"37 3","pages":"213-224"},"PeriodicalIF":2.3,"publicationDate":"2022-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10226087/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9545426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prognostic value of AKT isoforms in non-small cell lung adenocarcinoma.","authors":"Sahil Khurana,&nbsp;Ajay Pal Singh,&nbsp;Ashok Kumar,&nbsp;Rajeev Nema","doi":"10.7555/JBR.36.20220138","DOIUrl":"https://doi.org/10.7555/JBR.36.20220138","url":null,"abstract":"Dear Editor, Lung cancer is one of the most prevalent cancers in the world and has a high mortality rate. Lung cancer patients often have a poor prognosis, with a five-year survival rate of only about 16%. The International Agency for Research on Cancer reports that lung cancer was the main cause of cancer deaths in 2020, accounting for 1.80 million deaths. Due to the dismal overall prognosis of lung cancer, there is an urgent need to develop accurate and effective diagnostic tests that target specifically early oncogenic pathways in lung cancer patients to improve their prognosis. The two principal types of lung cancer are small cell lung carcinoma (SCLC) and non-small cell lung carcinoma (NSCLC), with NSCLC accounting for around 85% of all lung malignancies[1]. The region frequency and prevalence of the lung disease are controlled by both genotypic and phenotypic exposures. An accurate lung cancer diagnosis is essential for the patient's better survival for two main reasons: appropriate drug selection and effective treatment prediction. Histopathological diagnosis depends on cell shape and the nucleus-to-cytoplasm size ratio to distinguish SCLC from NSCLC. Surgical resection, aggressive or palliative radiation, and neoadjuvant chemotherapy are frequently used to treat lung cancer. In the modern era, gene-targeted treatments against tyrosine kinase inhibitors and antibodies against mutations in driver genes for lung cancer are being developed. Several mutations have been shown to be the most common in lung cancer. For example, mutations in the K-ras proto-oncogene cause 10% to 30% of lung adenocarcinoma (LUAD), while epidermal growth factor receptor mutations are more frequent in squamous cell lung cancer (SqCLC)[2]. Kaplan-Meier plotter (KM plotter) database (http://kmplot.com/analysis/) is a commonly used database for the real-time meta-analysis of published lung cancer microarray datasets to find survival biomarkers[3]. In a number of malignancies, the KM plotter has also been used to discover genes that may serve as possible prognostic indicators for postprogression survival (PPS), progression-free survival (PFS), and overall survival (OS)[3]. Many human malignancies, including lung cancer, have activated and overexpressed AKT isoforms[4]. AKT2 inhibition aids in the suppression of LUAD cell proliferation and colony expansion. Therefore, the significance of AKT isoforms in the diagnosis and prognosis of lung cancer was investigated in the current study. The association between gene specific mRNA expression and OS was analyzed by the KM plotter. Currently, gene expression and survival data from 1927 patients with a follow-up period of 20 years are available. Gene names of AKT isoforms (i.e., AKT1, AKT2, and AKT3) were entered into the KM plotter database to obtain survival plots. The association between mRNA expression levels of different AKT isoforms and the established clinicopathological features was studied. The patient data were linked t","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":"37 3","pages":"225-228"},"PeriodicalIF":2.3,"publicationDate":"2022-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10226088/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9543966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}