Journal of Biomedical Research最新文献

{"title":"CYP3A triggers BDE47-induced ferritinophagy and ferroptosis in spermatogenic cells through ROS-mediated m ⁶A regulation of ATG12.","authors":"Shuyu Xu, Wenxia Fan, Jiangxue Qian, Yuewen He, Chunjin Li, Chao Chen, Li Wang, Yongquan Yu, Chao Wang, Shoulin Wang","doi":"10.7555/JBR.40.20260159","DOIUrl":"10.7555/JBR.40.20260159","url":null,"abstract":"<p><p>Cytochrome P450 CYP3A (CYP3A) is among the most abundant hepatic cytochrome P450 subfamilies and also mediates the metabolism and toxicity of xenobiotics. Previous studies have reported that CYP3A is also expressed in the testis; however, its role and molecular mechanism in mediating male reproductive damage remain unclear. In this study, through <i>in vitro</i> and <i>in vivo</i> experiments, we demonstrated the role of CYP3A in 2,2',4,4'-tetrabromodiphenyl ether (BDE47)-induced reproductive toxicity. The results showed that BDE47 induced CYP3A expression in mouse testes, leading to oxidative stress and ferroptosis through excessive reactive oxygen species (ROS) and ferrous iron (Fe ²⁺) overload, which was demonstrated by CYP3A overexpression or knockdown experiments in GC-2 cells. Mechanistically, in addition to direct ROS generation during metabolic processing, ferritinophagy contributed to intracellular Fe ²⁺ accumulation. Specifically, BDE47-induced ROS was associated with reduced N6-methyladenosine (m ⁶A) modification of <i>Atg12</i> mRNA and increased autophagy-related (ATG12) expression, thereby promoting ferritin heavy chain 1 (FTH1) degradation and subsequent Fe ²⁺ overload. These results were further validated by experiments using hydrogen peroxide or antioxidants in GC-2 cells, as well as by <i>Atg12</i> haploinsufficiency in mice. Our findings demonstrate that CYP3A plays a critical role in male reproductive toxicity induced by BDE47.</p>","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":" ","pages":"364-382"},"PeriodicalIF":3.7,"publicationDate":"2026-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13438320/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148015719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of pregnancy outcomes and vaginal microbiota in women with endometriosis undergoing frozen embryo transfer using letrozole combined with HMG versus hormone replacement therapy with GnRH-a pretreatment.","authors":"Jie Zhang, Lei Dai, Chunyan Jiang, Yuxin Zhao, Xiang Ma, Yugui Cui, Jiayin Liu","doi":"10.7555/JBR.39.20250205","DOIUrl":"10.7555/JBR.39.20250205","url":null,"abstract":"<p><p>This study investigated differences in reproductive outcomes and vaginal microbiota profiles between two endometrial preparation protocols-letrozole (LE) combined with human menopausal gonadotropin (HMG) and hormone replacement therapy (HRT) with gonadotropin-releasing hormone agonist (GnRH-a) pretreatment-in women with endometriosis undergoing frozen embryo transfer (FET). Following 1∶1 propensity score matching, a total of 770 FET cycles were analyzed. No statistically significant differences were observed in live birth rates or clinical pregnancy rates between the two groups. However, the LE + HMG group showed a nonsignificant trend toward a lower miscarriage rate (13.7% <i>vs.</i> 19.8%, <i>P</i> = 0.070), as well as lower observed rates of cesarean delivery (64.9% <i>vs.</i> 75.4%, <i>P</i> = 0.020) and hypertensive disorders of pregnancy (4.8% <i>vs.</i> 10.1%, <i>P</i> = 0.039). Previous evidence suggests an association between GnRH-a treatment and reproductive tract microbiota composition. Given the clinical and ethical constraints of endometrial sampling during FET, vaginal microbiota was evaluated as a non-invasive indicator of lower reproductive tract microbial composition. In the prospective arm, vaginal samples from 55 women in the LE + HMG group and 50 in the GnRH-a HRT group were analyzed using 16S rRNA sequencing and droplet digital PCR. While no significant differences were observed in <i>Lactobacillus</i> or <i>Gardnerella</i> abundance, the GnRH-a HRT group showed higher relative abundances of several low-abundance taxa, such as <i>Escherichia-Shigella</i>, <i>Limosilactobacillus</i>, and <i>Staphylococcus</i>. In conclusion, although both protocols achieved comparable live birth outcomes, the LE + HMG regimen was associated with lower rates of cesarean delivery and hypertensive disorders of pregnancy, while the two protocols exhibited differences in several low-abundance vaginal bacterial taxa. However, longitudinal studies incorporating baseline and repeated sampling are needed to clarify temporal relationships and causality.</p>","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":" ","pages":"395-409"},"PeriodicalIF":3.7,"publicationDate":"2026-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13438300/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144215834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The association between HPV infection and endometriosis: Risk and fertility outcomes.","authors":"Wenqu Li, Zhenlong Wang, Huizhi Qi, Chengjie Xia, Feixue Chen, Juan Xu, Lei Zhang, Xuemei Jia","doi":"10.7555/JBR.39.20250194","DOIUrl":"10.7555/JBR.39.20250194","url":null,"abstract":"<p><p>While infections have been implicated in endometriosis pathogenesis, the role of human papillomavirus (HPV) remains unclear. This study combined a meta-analysis of seven studies with a case-control study ( <i>n</i> = 432 surgically treated patients) to evaluate the association of HPV infection with endometriosis risk and fertility outcomes. The meta-analysis showed no significant association between HPV infection and endometriosis risk, either for any HPV subtype (pooled odds ratio [OR] = 2.60, 95% confidence interval [CI]: 0.28-23.87) or high-risk subtypes (OR = 1.68, 95% CI: 0.49-5.75). Notably, HPV prevalence was higher in patients with endometriosis (46% overall; 36% for high-risk subtypes) than in the general population. Evidence regarding the association between HPV infection and fertility outcomes remains limited, with only two eligible studies identified. Our case-control analysis revealed that HPV-positive patients had significantly reduced postoperative live birth rates compared with HPV-negative counterparts (10.6% <i>vs.</i> 21.0%, <i>P</i> < 0.05). These findings suggest that HPV infection is unlikely to be a major risk factor for endometriosis development, but it may be associated with poorer postsurgical fertility outcomes. The dual-method approach strengthens the evidence for HPV's clinical impact on reproductive prognosis in patients with existing endometriosis.</p>","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":" ","pages":"383-394"},"PeriodicalIF":3.7,"publicationDate":"2026-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13438304/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145258469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Maternal and neonatal outcomes among Chinese women meeting NICE but not IADPSG criteria for gestational diabetes mellitus: A retrospective cohort study.","authors":"Haijuan Yu, Yuan Qin, Runlian Tao, Yuanyuan Chen, Li Yuan, Lizhou Sun","doi":"10.7555/JBR.39.20250350","DOIUrl":"10.7555/JBR.39.20250350","url":null,"abstract":"<p><p>In China, gestational diabetes mellitus (GDM) is typically diagnosed using the International Association of Diabetes and Pregnancy Study Groups (IADPSG) criteria. This study retrospectively analyzed 19152 pregnant women who underwent an oral glucose tolerance test between 2015 and 2021, comparing the IADPSG and the National Institute for Health and Care Excellence (NICE) criteria. GDM prevalence was 20.39% (IADPSG) and 23.67% (NICE, <i>P</i> < 0.01), with a moderate diagnostic agreement (κ = 0.59; <i>P</i> < 0.01). Compared with women having normal glucose tolerance (NGT group), women diagnosed with GDM by either the IADPSG (GDM-I) or NICE (GDM-N) criteria had significantly higher risks of adverse outcomes (including gestational hypertension, preeclampsia, preterm delivery, macrosomia, low birth weight, and neonatal jaundice). However, those diagnosed by NICE but not by IADPSG (GDM-NI) showed no significant increase in adverse maternal or neonatal outcomes except for neonatal jaundice. Furthermore, the GDM-NI group had significantly lower rates of several adverse outcomes compared with the GDM-I group. These findings suggest that, under current clinical management where women diagnosed by IADPSG received treatment while those diagnosed only by NICE did not, the IADPSG criteria classified fewer women as having GDM but identified a group with a higher observed risk of several adverse pregnancy outcomes than did the NICE criteria.</p>","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":" ","pages":"436-447"},"PeriodicalIF":3.7,"publicationDate":"2026-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13438299/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145742376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stratified profiling of azoospermia: Differentiating histological subtypes with seminal plasma metabolic signatures.","authors":"Jiachen Wang, Tianyi Song, Hongqi Fan, Mengyuan Zhu, Ying Yao, Laihua Li, Mingxi Liu, Minjian Chen, Jiahao Sha, Xiaoyu Yang, Yan Yuan","doi":"10.7555/JBR.39.20250294","DOIUrl":"10.7555/JBR.39.20250294","url":null,"abstract":"<p><p>Non-obstructive azoospermia (NOA), characterized by impaired spermatogenesis and the complete absence of sperm in the ejaculate, represents one of the most severe forms of male infertility. Current approaches for definitive histological characterization and sperm retrieval often require invasive testicular procedures, underscoring the need for reliable, non-invasive methods for preoperative stratification. In the present study, we performed untargeted metabolomic profiling of human seminal plasma to identify biomarker panels capable of stratifying azoospermia subtypes through a stepwise approach. We identified distinct metabolite biomarker panels comparing NOA <i>vs.</i> obstructive azoospermia (OA), Sertoli cell-only syndrome <i>vs.</i> other NOA subtypes, and hypospermatogenesis <i>vs.</i> maturation arrest. Additionally, cross-species comparative analysis with high-resolution metabolomic data from purified mouse testicular cell populations implicated these biomarkers in specific germ cell stages and metabolic transitions during spermatogenesis. These findings provide a preliminary molecular framework for the non-invasive classification of azoospermia subtypes and warrant validation in larger independent cohorts.</p>","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":" ","pages":"425-435"},"PeriodicalIF":3.7,"publicationDate":"2026-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13438305/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145033259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial commentary on the special issue of reproductive medicine.","authors":"Editorial Board","doi":"10.7555/JBR.40.20268003","DOIUrl":"10.7555/JBR.40.20268003","url":null,"abstract":"","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":"40 4","pages":"345-346"},"PeriodicalIF":3.7,"publicationDate":"2026-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13438302/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148663894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbiota-metabolite axis in endometriosis: Pathogenic mechanisms and clinical implications.","authors":"Moyuan Li, Aiyuan Yue, Lingfeng Gu, Feiyang Li, Nuo Ye, Sujuan Xu, Zhen Gong, Dake Li, Pengfei Xu","doi":"10.7555/JBR.39.20250566","DOIUrl":"10.7555/JBR.39.20250566","url":null,"abstract":"<p><p>Growing evidence highlights the gut, reproductive tract, and endometrial microbiota as important functional contributors in the pathogenesis of endometriosis (EM). Studies have revealed a characteristic microbial imbalance in patients with EM, marked by a reduced abundance of beneficial bacteria and an enrichment of opportunistic pathogens. These microbial communities are thought to influence disease progression primarily through metabolic activity, as demonstrated by metabolomic studies showing their capacity to modulate host immune and endocrine responses. This imbalance may contribute to several key metabolic disturbances, including decreased levels of short-chain fatty acids, particularly butyrate; a shift in tryptophan metabolism toward the kynurenine pathway; elevated β-glucuronidase activity; increased lipopolysaccharide production; and altered secondary bile acid profiles. Functionally, these metabolic alterations are thought to contribute to EM by disrupting immune homeostasis, enhancing estrogen signaling, and driving systemic inflammation, thereby creating a permissive microenvironment for ectopic lesion growth and invasion. Targeted interventions, such as probiotics, high-fiber dietary strategies, fecal microbiota transplantation, and selective modulation of microbial or host metabolic enzymes, are emerging as promising non-hormonal therapeutic approaches. Nonetheless, further studies incorporating longitudinal cohort designs and integrative multi-omics approaches are essential to establish causality and facilitate the development of precise diagnostic and personalized treatment strategies.</p>","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":" ","pages":"347-363"},"PeriodicalIF":3.7,"publicationDate":"2026-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13438310/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147772224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CRISPR/Cas9-mediated genome editing identifies six testis-expressed genes individually dispensable for overt male fecundity in mice.","authors":"Heling Fu, Haoran Chen, Chenmeijie Li, Shuai Lu, Yayun Gu, Zhibin Hu","doi":"10.7555/JBR.39.20250247","DOIUrl":"10.7555/JBR.39.20250247","url":null,"abstract":"<p><p>The genetic landscape of male infertility is highly complex. It is estimated that at least 1000-2000 genes are involved in human and mouse infertility. Although functional analyses have been performed on hundreds of genes, many others still have unknown functions. Generating gene-editing mice is a powerful tool for exploring whether a given gene is essential for male reproduction <i>in vivo</i>. In the current study, we investigated the function of six genes, <i>Efcab7</i>, <i>Tekt3</i>, <i>Mlf1</i>, <i>Rp1l1</i>, <i>Agbl2</i>, and <i>Tmsb15a</i>, using the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) system. Individual disruption of these six genes did not cause overt male sterility in mice under standard breeding conditions. Phenotypic analyses at 8-10 weeks of age generally showed that most reproductive parameters were comparable to those of wild-type controls; however, we observed significant sperm motility alterations in <i>Tekt3</i>-mutant mice (decreased motility) and <i>Rp1l1</i>-mutant mice (increased motility), as well as flagellar abnormalities in <i>Tekt3</i> mutants. In summary, these findings suggest that complete or near-complete disruption of the tested murine alleles does not cause overt male infertility, providing useful functional evidence for interpretation of candidate variants while not excluding human-specific or allele-specific effects.</p>","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":" ","pages":"410-424"},"PeriodicalIF":3.7,"publicationDate":"2026-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13438311/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145451958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bisphenol A impairs contractile function and vascular development in human cardiac organoids: Insights from toxicity assessment and evidence-based risk evaluation.","authors":"Linfeng Jiang, Yachang Wang, Huina Li, Yang Yang","doi":"10.7555/JBR.39.20250533","DOIUrl":"10.7555/JBR.39.20250533","url":null,"abstract":"<p><p>Bisphenol A (BPA), a widely encountered environmental endocrine-disrupting chemical, has been linked to an increased risk of congenital heart defects in epidemiological and experimental studies. To investigate its impact in a human-relevant system, we used a human cardiac organoid (hCO) model derived from human embryonic stem cells and exposed hCOs to BPA concentrations ranging from environmentally relevant to higher experimental levels (0.1-10 μmol/L) throughout differentiation. BPA exposure impaired cardiac contractile function and disrupted endothelial network formation, with more pronounced functional effects at higher concentrations. BPA exposure also induced apoptosis in hCOs. Subsequently, transcriptomic profiling revealed dysregulation of genes associated with cardiac rhythm and developmental signaling in BPA-treated hCOs. Among the concentrations tested, 0.5 μmol/L was the lowest concentration associated with a significant reduction in CD31 fluorescence intensity. This research provides <i>in vitro</i> evidence that BPA can perturb key processes in early human heart development, highlighting the necessity for further mechanistic and <i>in vivo</i> studies to clarify exposure relevance and its potential implications for fetal heart health.</p>","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":" ","pages":"448-462"},"PeriodicalIF":3.7,"publicationDate":"2026-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13438319/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147573844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bronchial mucous gland adenoma: a rare case report and literature review.","authors":"Wang Wan, ZhuoHong Ye, GuoXiong Zeng, Heng Jiang, Biao Deng, XueFeng Wang, YanXia Wu, Zhu Liang","doi":"10.7555/JBR.39.20250534","DOIUrl":"https://doi.org/10.7555/JBR.39.20250534","url":null,"abstract":"","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":" ","pages":"1-7"},"PeriodicalIF":3.7,"publicationDate":"2026-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148678632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}