Journal of Biomedical Research最新文献

Editorial commentary on the special issue of cancer research. 关于癌症研究特刊的社论评论。

IF 2.4 4区医学

Journal of Biomedical Research Pub Date : 2025-09-25 DOI: 10.7555/JBR.39.20250800

引用次数: 0

Where do I submit my next paper? A practical guide for biomedical researchers. 我的下一篇论文在哪里提交？生物医学研究人员的实用指南。

IF 2.4 4区医学

Journal of Biomedical Research Pub Date : 2025-09-04 DOI: 10.7555/JBR.39.20250147

Mohammad S Alrashdan

引用次数: 0

STK11 rs12977689 C>A gene polymorphism as a risk factor for coronary artery disease in type 2 diabetes patients. STK11 rs12977689 C>A基因多态性与2型糖尿病患者冠状动脉疾病的危险因素

IF 2.4 4区医学

Journal of Biomedical Research Pub Date : 2025-09-04 DOI: 10.7555/JBR.39.20250136

Made Edwin Sridana, Wira Gotera, Bagus Ari, Pradnyana Dwi, Bagus Ari Pradnyana Dwi Sutanagera, Made Ratna Saraswati

引用次数: 0

Stratified profiling of azoospermia: Differentiating histological subtypes with seminal plasma metabolic signatures. 无精子症的分层分析：用精浆代谢特征区分组织学亚型。

IF 2.4 4区医学

Journal of Biomedical Research Pub Date : 2025-09-04 DOI: 10.7555/JBR.39.20250294

Jiachen Wang, Tianyi Song, Hongqi Fan, Mengyuan Zhu, Ying Yao, Laihua Li, Mingxi Liu, MinJian Chen, Jiahao Sha, Xiaoyu Yang, Yan Yuan

{"title":"Stratified profiling of azoospermia: Differentiating histological subtypes with seminal plasma metabolic signatures.","authors":"Jiachen Wang, Tianyi Song, Hongqi Fan, Mengyuan Zhu, Ying Yao, Laihua Li, Mingxi Liu, MinJian Chen, Jiahao Sha, Xiaoyu Yang, Yan Yuan","doi":"10.7555/JBR.39.20250294","DOIUrl":"https://doi.org/10.7555/JBR.39.20250294","url":null,"abstract":"Non-obstructive azoospermia (NOA), characterized by impaired spermatogenesis and the complete absence of sperm in the ejaculate, represents one of the most severe forms of male infertility. Current diagnostic strategies rely on invasive procedures such as testicular sperm extraction, underscoring the urgent need for reliable, non-invasive alternatives. In the present study, we performed untargeted metabolomic profiling of human seminal plasma to identify biomarker panels capable of stratifying azoospermia subtypes through a stepwise approach. We identified distinct metabolite biomarker panels comparing NOA vs. obstructive azoospermia (OA), Sertoli cell-only syndrome vs. other NOA subtypes, and hypospermatogenesis vs. maturation arrest. Additionally, cross-species comparative analysis with high-resolution metabolomic data from purified mouse testicular cell populations implicated these biomarkers in specific germ cell stages and metabolic transitions during spermatogenesis. These findings establish a molecular framework for the non-invasive classification of azoospermia subtypes and provide novel insights into the metabolic disruptions underlying male infertility.","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":" ","pages":"1-11"},"PeriodicalIF":2.4,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145033259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrating bulk and single-cell sequencing reveals cellular heterogeneity between lung adenocarcinoma in smokers and never-smokers. 整合整体和单细胞测序揭示了吸烟者和不吸烟者肺腺癌之间的细胞异质性。

IF 2.4 4区医学

Journal of Biomedical Research Pub Date : 2025-08-25 DOI: 10.7555/JBR.39.20250160

Zihuan Zhao, Pan Yang, Yuhan Liu, Kai Wang, Xianfeng Xu, Yuzhuo Wang, Meng Zhu, Na Qin, Cheng Wang, Weimin Li, Hongxia Ma, Zhoufeng Wang, Hongbing Shen

{"title":"Integrating bulk and single-cell sequencing reveals cellular heterogeneity between lung adenocarcinoma in smokers and never-smokers.","authors":"Zihuan Zhao, Pan Yang, Yuhan Liu, Kai Wang, Xianfeng Xu, Yuzhuo Wang, Meng Zhu, Na Qin, Cheng Wang, Weimin Li, Hongxia Ma, Zhoufeng Wang, Hongbing Shen","doi":"10.7555/JBR.39.20250160","DOIUrl":"https://doi.org/10.7555/JBR.39.20250160","url":null,"abstract":"Lung cancer in smokers (LCIS) and lung cancer in never-smokers (LCINS) are different entities with distinct molecular features. However, their cellular heterogeneity still requires further investigation. Through an integrated analysis of single-cell RNA sequencing (scRNA-seq) and bulk sequencing data, we identified cell subpopulations associated with smoking and non-smoking patients. Downstream transcriptomic analyses were then performed to reveal differences in cell function and tumor microenvironment. We observed that smoking-associated cancer cells exhibited a higher degree of aggressiveness, which may correlate with an adverse prognosis in smoking patients. Additionally, immunosuppressive CXCL10+ macrophages may be involved in their tumorigenesis in smokers, and the immunoregulatory LGALS9-HAVCR2 axis could be a potential immunotherapeutic target. In non-smokers, the inflammatory microenvironment may be involved in their tumor formation. Moreover, the decreased anti-tumor cytotoxicity could be associated with their suboptimal immunotherapeutic response. Our study uncovered differences in oncogenic and immune escape mechanisms between LCIS and LCINS patients and suggests potential immunotherapy strategies.","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":" ","pages":"1-18"},"PeriodicalIF":2.4,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144955231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The mediating effect of blood pressure between healthy lifestyles and stroke: Results from the China Kadoorie Biobank study. 血压在健康生活方式和中风之间的中介作用：来自中国嘉道理生物银行的研究结果。

IF 2.4 4区医学

Journal of Biomedical Research Pub Date : 2025-08-25 DOI: 10.7555/JBR.39.20250177

Zidong Wang, Jiaxi Zhou, Xikang Fan, Jian Su, Houyue Geng, Xun Wu, Yujie Hua, Hongfu Ren, Jun Lv, Pei Pei, Canqing Yu, Dianjianyi Sun, Yan Lu, Jinyi Zhou, Ran Tao

{"title":"The mediating effect of blood pressure between healthy lifestyles and stroke: Results from the China Kadoorie Biobank study.","authors":"Zidong Wang, Jiaxi Zhou, Xikang Fan, Jian Su, Houyue Geng, Xun Wu, Yujie Hua, Hongfu Ren, Jun Lv, Pei Pei, Canqing Yu, Dianjianyi Sun, Yan Lu, Jinyi Zhou, Ran Tao","doi":"10.7555/JBR.39.20250177","DOIUrl":"https://doi.org/10.7555/JBR.39.20250177","url":null,"abstract":"While a healthy lifestyle is known to reduce stroke risk, the extent to which blood pressure (BP) mediates this association remains unclear. The present study aimed to quantify the mediating role of BP in the association between combined lifestyle factors and stroke incidence. Using data from 51929 participants free of major cardiovascular diseases or cancer at baseline, we employed structural equation modeling to assess the mediating effects of systolic (SBP) and diastolic (DBP) blood pressure. During the follow-up, 2811 incident stroke cases were identified. A healthy lifestyle was significantly associated with a reduced risk of stroke, with SBP mediating 44.70% ( β = - 0.0014, 95% confidence interval [CI]: - 0.0016 to - 0.0012) and DBP mediating 37.81% ( β = - 0.0012, 95% CI: - 0.0015 to - 0.0009) of this association. The mediating effects were attenuated but remained significant for ischemic stroke (SBP: 33.21%; DBP: 27.24%). In conclusion, approximately two-fifths of the protective association between a healthy lifestyle and stroke may be mediated by BP. These findings suggest that BP control may serve as an important early indicator for evaluating the effectiveness of lifestyle interventions in reducing stroke risk.","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":" ","pages":"1-9"},"PeriodicalIF":2.4,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144955261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cervical cancer perceived risk factors behavior using logistic regression technique. 应用logistic回归技术分析宫颈癌感知危险因素行为。

IF 2.4 4区医学

Journal of Biomedical Research Pub Date : 2025-08-25 DOI: 10.7555/JBR.39.20250047

I M Elzein, Achraf Chamseddine, Ahmad Eltanboly, Adam Elzein

{"title":"Cervical cancer perceived risk factors behavior using logistic regression technique.","authors":"I M Elzein, Achraf Chamseddine, Ahmad Eltanboly, Adam Elzein","doi":"10.7555/JBR.39.20250047","DOIUrl":"https://doi.org/10.7555/JBR.39.20250047","url":null,"abstract":"Cervical cancer represents a considerable global health challenge, mainly because of ineffective screening programs in middle-income countries. The current study aimed to forecast cervical cancer incidence by analyzing behavioral risk factors through logistic regression, employing feature engineering techniques such as principal component analysis (PCA). PCA successfully condensed the dataset into ten principal components, capturing 89% of the variance, while stratified K-fold cross-validation ensured a balanced representation of classes. With the application of L1 regularization, the logistic regression model achieved an accuracy of 97.2%, an AUC of 98.1%, an F1 score of 97.2%, a specificity of 96.1%, and a log loss of 0.17. The performance of models was comparatively evaluated, and the results revealed that the logistic regression model achieved the highest accuracy of 97.2% in comparison with decision trees at 93.33%, random forest at 93.33%, XGBoost at 93.33%, Naive Bayes at 91.67%, and non-regularized logistic regression at 87.55%. This research underscores the importance of early prediction of cervical cancer based on behavioral risk factors and suggests a robust, easily implementable workflow to improve classification accuracy. Future research should concentrate on refining these predictive tools to overcome social and behavioral barriers to prevention, particularly within underserved populations.","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":" ","pages":"1-12"},"PeriodicalIF":2.4,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144955240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association between psoriasis and chronic bronchitis: Adverse reaction to therapy or neglected comorbidity? 银屑病与慢性支气管炎的关系：治疗不良反应或被忽视的合并症？

IF 2.4 4区医学

Journal of Biomedical Research Pub Date : 2025-08-25 DOI: 10.7555/JBR.39.20250178

Xinyi Dai, Chenxingyue Zhang, Zhiqiang Yin

引用次数: 0

hUMSC-derived exosomes alleviate hypoxic cerebrovascular injury via AMPK/NLRP3-mediated pyroptosis suppression and mitochondrial protection. humsc衍生的外泌体通过AMPK/ nlrp3介导的焦亡抑制和线粒体保护减轻缺氧脑血管损伤。

IF 2.4 4区医学

Journal of Biomedical Research Pub Date : 2025-08-25 DOI: 10.7555/JBR.39.20250189

Jinteng Liu, Yunlong Pan, Haolin Wu, Qingyun Guo, Xingyue Fang, Yingmei Lu, Qibing Liu

{"title":"hUMSC-derived exosomes alleviate hypoxic cerebrovascular injury via AMPK/NLRP3-mediated pyroptosis suppression and mitochondrial protection.","authors":"Jinteng Liu, Yunlong Pan, Haolin Wu, Qingyun Guo, Xingyue Fang, Yingmei Lu, Qibing Liu","doi":"10.7555/JBR.39.20250189","DOIUrl":"https://doi.org/10.7555/JBR.39.20250189","url":null,"abstract":"Ranked as the most prevalent cause of death worldwide, ischemic stroke urgently requires innovative therapeutic strategies. The present study demonstrates the therapeutic potential of human umbilical cord-derived mesenchymal stem cell-derived exosomes (hUMSC-Exos) in ameliorating hypoxia-induced cerebrovascular endothelial dysfunction through modulation of the AMPK/NLRP3 signaling pathway. Bioinformatics analysis of DisGeNET and exosomal cargo databases revealed 283 overlapping cerebral ischemia-related genes, implicating hUMSC-Exos in inflammatory regulation. In vitro experiments showed that hUMSC-Exos rescued oxygen-glucose deprivation (OGD)-induced endothelial dysfunction in bEnd.3 mouse brain endothelial cells, restoring viability, migration, and mitochondrial integrity. Mechanistically, hUMSC-Exos reversed OGD-induced AMPK inactivation while suppressing NLRP3 inflammasome activation, caspase-1 cleavage, and gasdermin D (GSDMD)-mediated pyroptosis. Molecular docking revealed DL-3-n-butylphthalide as a dual-target ligand for AMPK/NLRP3, synergizing with hUMSC-Exos to enhance endothelial protection. In vivo, combined therapy in the transient middle cerebral artery occlusion mouse model reduced cerebral infarction and improved neurological outcomes, accompanied by NLRP3/GSDMD downregulation and hippocampal neuron preservation. These findings establish hUMSC-Exos as regulators of AMPK/NLRP3-mediated pyroptosis and propose a translatable combinatorial regimen for ischemic stroke therapy.","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":" ","pages":"1-15"},"PeriodicalIF":2.4,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144955215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Human serum albumin modified in myeloperoxidase-dependent reactions is a mediator of neutrophil extracellular trap formation. 髓过氧化物酶依赖性反应中修饰的人血清白蛋白是中性粒细胞胞外陷阱形成的介质。

IF 2.2 4区医学

Journal of Biomedical Research Pub Date : 2025-07-25 DOI: 10.7555/JBR.39.20250069

Daria V Grigorieva, Nikolay P Gorbunov, Valeria A Kostevich, Alexey V Sokolov, Liliya Yu Basyreva, Ekaterina V Shmeleva, Tatyana V Vakhrusheva, Sergey A Gusev, Irina V Gorudko, Oleg M Panasenko

{"title":"Human serum albumin modified in myeloperoxidase-dependent reactions is a mediator of neutrophil extracellular trap formation.","authors":"Daria V Grigorieva, Nikolay P Gorbunov, Valeria A Kostevich, Alexey V Sokolov, Liliya Yu Basyreva, Ekaterina V Shmeleva, Tatyana V Vakhrusheva, Sergey A Gusev, Irina V Gorudko, Oleg M Panasenko","doi":"10.7555/JBR.39.20250069","DOIUrl":"https://doi.org/10.7555/JBR.39.20250069","url":null,"abstract":"Activation of neutrophil membrane receptors initiates intracellular signal transduction cascades that orchestrate the cell's effector functions, including phagocytosis, production of reactive oxygen and halogen species, degranulation, and NETosis (formation of neutrophil extracellular traps (NETs). NETs, which contain antimicrobial compounds such as myeloperoxidase (MPO), represent a strategy to combat infection. However, excessive production of NETs promotes thrombosis, diabetes mellitus, and other diseases. Therefore, investigations into the mechanisms of NETosis and the identification of modulators of this process are critical for developing strategies to address NETosis-related disorders. Here, we identified a novel NETosis inducer, human serum albumin (HSA) modified by the MPO product hypochlorous acid (HSA HOCl), whose accumulation in vivo was correlated with inflammatory processes. Using human blood neutrophils, we investigated HSA HOCl-induced NETosis and detected NET formation by flow cytometry. The results showed that the mechanism of HSA HOCl-induced NETosis involved MPO, NADPH oxidase and phosphatidylinositol 3-kinases, and that HSA HOCl activated a reactive oxygen species-dependent suicidal type of NETosis. Moreover, HSA HOCl-induced NETosis was inhibited by an anti-HSA HOCl monoclonal antibody. Thus, our findings may facilitate the development of strategies to modulate NETosis in inflammation correlated with elevated MPO activity.","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":" ","pages":"1-12"},"PeriodicalIF":2.2,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144690373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0