Journal of Biomedical Research最新文献

{"title":"Evaluation of the plasma antioxidant activity in women with early pregnancy losses.","authors":"Olga Gennadievna Tishkova, Lydmila Vasilievna Dikareva, Nadezhda Titovna Berberova, Maria Alexandrovna Polovinkina, Victoria Pavlovna Osipova","doi":"10.7555/JBR.38.20240396","DOIUrl":"https://doi.org/10.7555/JBR.38.20240396","url":null,"abstract":"","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":" ","pages":"1-4"},"PeriodicalIF":2.2,"publicationDate":"2025-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144150532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect and toxicity profile of consolidative or salvage thoracic radiotherapy following chemoimmunotherapy in patients with extensive stage small cell lung cancer.","authors":"Ruo-Zhou Sun, Dan Zong, Xin Chen, Yi-Zhi Ge, Ning Jiang, Li-Jun Zhao, Xue Song, Xia He, Xiang-Zhi Zhu","doi":"10.7555/JBR.39.20250067","DOIUrl":"https://doi.org/10.7555/JBR.39.20250067","url":null,"abstract":"<p><p>This study evaluated the efficacy and safety of thoracic radiotherapy (TRT) after first-line chemotherapy or chemoimmunotherapy in patients with extensive-stage small cell lung cancer (ES-SCLC), focusing on the impact of different TRT timing strategies (consolidative vs. salvage) on survival. A total of 54 ES-SCLC patients treated between January 2019 and July 2022 were retrospectively analyzed. Patients receiving consolidative TRT (cTRT) within 3 months after first-line treatment completion were compared to those receiving salvage TRT (sTRT) following disease progression. Primary endpoints included overall survival (OS), progression-free survival (PFS), locoregional-free survival (LRFS), and distant metastasis-free survival (DMFS); safety was a secondary endpoint. The cTRT group (n=41) showed significantly longer median OS (26.6 vs. 14.8 months, P=0.048), PFS (12.9 vs. 3.5 months, P< 0.0001), and DMFS (10.7 vs. 3.4 months, P= 0.0044) than the sTRT group (n=13). Multivariate analysis identified cTRT as an independent favorable prognostic factor. No significant differences in OS or LRFS were found between high-dose (≥50 Gy) and low-dose (<50 Gy) TRT. Hematologic and respiratory toxicities were the most common adverse events, with acceptable tolerability. In conclusion, consolidative TRT after chemoimmunotherapy significantly improves survival outcomes in ES-SCLC patients, and low-dose TRT may be a suitable option.</p>","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":" ","pages":"1-11"},"PeriodicalIF":2.2,"publicationDate":"2025-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144150556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrative omics and multi-cohort identify <i>IRF1</i> and biological targets related to sepsis-associated acute respiratory distress syndrome.","authors":"Jiajin Chen, Ruili Hou, Xiaowen Xu, Ning Xie, Jiaqi Tang, Yi Li, Xiaoqing Nie, Nuala J Meyer, Li Su, David C Christiani, Feng Chen, Ruyang Zhang","doi":"10.7555/JBR.39.20250066","DOIUrl":"https://doi.org/10.7555/JBR.39.20250066","url":null,"abstract":"<p><p>Interferon-related genes are involved in antiviral responses, inflammation, and immunity, which are closely related to sepsis-associated acute respiratory distress syndrome (ARDS). We analyzed 1972 participants with genotype data and 681 with gene expression data from the Molecular Epidemiology of ARDS (MEARDS), the Molecular Epidemiology of Sepsis in the ICU (MESSI), and the Molecular Diagnosis and Risk Stratification of Sepsis (MARS) cohorts in a three-step study focusing on sepsis-associated ARDS and sepsis-only controls. First, we identified and validated interferon-related genes associated with sepsis-associated ARDS risk using genetically regulated gene expression (GReX). Second, we examined the association of the confirmed gene (interferon regulatory factor 1, <i>IRF1</i>) with ARDS risk and survival and conducted a mediation analysis. Through discovery and validation, we found that the GReX of <i>IRF1</i> was associated with ARDS risk (OR _MEARDS = 0.84, <i>P</i> = 0.008; OR _MESSI = 0.83, <i>P</i> = 0.034). Furthermore, individual-level measured <i>IRF1</i> expression was associated with reduced ARDS risk (OR = 0.58, <i>P</i> = 8.67×10 ^-4), and improved overall survival in ARDS patients (HR _28-day = 0.49, <i>P</i> = 0.009) and sepsis patients (HR _28-day = 0.76, <i>P</i> = 0.008). Mediation analysis revealed that <i>IRF1</i> may activate immune functions by regulating the major histocompatibility complex, including <i>HLA-F</i>, which mediated over 70% of the protective effects of <i>IRF1</i> on ARDS. The findings were validated by <i>in vitro</i> biological experiments involving time-series infection dynamics, overexpression, knockout, and chromatin immunoprecipitation sequencing. Early prophylactic interventions to activate <i>IRF1</i> in sepsis patients, thereby regulating <i>HLA-F</i>, might reduce the risk of ARDS development and mortality, especially in severely illness patients.</p>","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":" ","pages":"1-12"},"PeriodicalIF":2.2,"publicationDate":"2025-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144150553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Washed microbiota transplantation for ribotype 027 <i>Clostridioides difficile</i> infection in a pregnant woman with two-year follow-up: a case report.","authors":"Xinyi He, Sibusiso Luthuli, Quan Wen, Chuan Wang, Jinli Ding, Bota Cui, Faming Zhang","doi":"10.7555/JBR.39.20250063","DOIUrl":"https://doi.org/10.7555/JBR.39.20250063","url":null,"abstract":"<p><p><i>Clostridioides difficile</i> ( <i>C. difficile</i>) is one of the major causes of nosocomial infections. The pregnant women, who were considered at low risk for <i>C. difficile</i> infection (CDI), have attracted attention with increasing reports. Oral vancomycin, the only first-line treatment for the pregnant women infected with <i>C. difficile</i>, came with the problem of increasing strains resistance that was associated with decreased efficacy. Fecal microbiota transplantation (FMT) is recommended for severe, fulminant and recurrent CDI, while it is avoided in the pregnant women due to safety concerns. We reported a pregnant woman case with primary ribotype 027 CDI, who got a successful outcome with washed microbiota transplantation (WMT), an improved FMT, <i>via</i> enema. The specific strain of ribotype 027 was related to severe outcomes but was not reported in the pregnant women. The follow-up lasted two years, the patient's diarrhea was fully alleviated without recurrence. The baby had normal growth and development and no adverse events were recorded in both of them. This case provides evidence for the efficacy and safety of WMT in the pregnant women infected with <i>C. difficile,</i> indicating that WMT <i>via</i> enema may be a strategy for this population in treating CDI.</p>","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":" ","pages":"1-4"},"PeriodicalIF":2.2,"publicationDate":"2025-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144150557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DEC1 promotes breast cancer bone metastasis through transcriptional activation of CXCR4.","authors":"Ying Huo, Kaiao Chen, Zhiyi Qiang, Lan Lin, Wei Liu, Jian Yang","doi":"10.7555/JBR.39.20250031","DOIUrl":"https://doi.org/10.7555/JBR.39.20250031","url":null,"abstract":"<p><p>Bone metastasis is the primary cause of mortality in breast cancer (BC) patients. This study elucidates the functional role of DEC1 (differentiated embryonic chondrocyte expressed gene 1) in promoting BC bone metastasis. Analysis of patient-derived samples and public databases revealed significant upregulation of DEC1 and CXCR4 in breast tumors compared to adjacent normal tissues, with elevated levels correlating with increased metastatic potential, suggesting their synergistic involvement in BC progression. Intracardiac injection experiments demonstrated that 4T1-WT cells induced more severe osteolysis and larger metastatic lesions than 4T1-DEC1-KD cells. In MDA-MB-231 cells, DEC1 overexpression (OE) upregulated CXCR4 and proliferation/migration-related genes, whereas DEC1 knockdown (KD) suppressed these effects. Notably, AMD3100 (a CXCR4 antagonist) partially reversed the DEC1-OE-induced upregulation of CXCR4 and associated pro-metastatic genes. Mechanistically, DEC1 was found to bind the CXCR4 promoter region (-230 to -326) and activate its transcription, corroborated by ChIP-seq data. Furthermore, pharmacological inhibition of AKT (LY294002) or JAK2 (AZD1480), but not ERK (PD98059), attenuated DEC1-mediated CXCR4 upregulation, although all three inhibitors mitigated DEC1-driven migration-related gene expression. Additionally, DEC1 enhanced CXCL12 secretion from mesenchymal stromal cells and osteoblasts, amplifying the CXCR4/CXCL12 axis within the bone microenvironment. Collectively, our findings demonstrate that DEC1 promotes breast cancer (BC) bone metastasis by directly transactivating CXCR4 expression, providing a molecular basis for targeting DEC1 to prevent and treat BC bone metastasis.</p>","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":" ","pages":"1-22"},"PeriodicalIF":2.2,"publicationDate":"2025-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144150530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phenotype and genotype analyses of 21 Chinese patients with Dent disease.","authors":"Ruochen Che, Yuwen Cai, Wei Zhou, Sanlong Zhao, Songming Huang","doi":"10.7555/JBR.38.20240183","DOIUrl":"https://doi.org/10.7555/JBR.38.20240183","url":null,"abstract":"<p><p>Dent disease is a rare X-linked recessively inherited renal tubulopathy, caused by variants in <i>CLCN5</i> (Dent disease type 1, DD1) and <i>OCRL</i> (Dent disease type 2, DD2) and characterized by low molecular weight proteinuria, hypercalciuria, microscopic hematuria, or nephrocalcinosis. In the current study, we collected and analyzed clinical data and genetic testing results of 21 children diagnosed with Dent disease, who were hospitalized at the Department of Nephrology, Children's Hospital of Nanjing Medical University between January 2014 and August 2023, aiming to assist in the early diagnosis and treatment of the patients. Among the 21 patients, 16 (76.19%) had <i>CLCN5</i> variants and five (23.81%) had <i>OCRL</i> variants, and four of the variants were novel. All patients presented with low-molecular-weight proteinuria, 14 (66.67%) of whom had nephrotic-range proteinuria. Eight patients underwent renal biopsies because of diagnostic uncertainty. We transfected the novel <i>CLCN5</i> missense variant (p.G222R) and <i>OCRL</i> missense variant (p.I371T) plasmids into both HEK293 and HK-2 cells and found a significantly lower expression of the OCRL1 protein in the transfected cells than the wild-type cells ( <i>P</i> < 0.05). Moreover, we observed an extremely skewed X-chromosome inactivation pattern in a female patient carrying the same novel <i>CLCN5</i> variant, as assessed by the human androgen receptor gene assay. These findings provide insight into clinical characteristics of Dent disease in Chinese patients and may shed light on its pathogenesis.</p>","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":" ","pages":"1-11"},"PeriodicalIF":2.2,"publicationDate":"2025-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144150555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between serum 25(OH)D and cancer in adults with psoriasis: a cross-sectional study.","authors":"Lingquan Deng, Yamei Gao, Chenxingyue Zhang, Zhiqiang Yin","doi":"10.7555/JBR.39.20250051","DOIUrl":"https://doi.org/10.7555/JBR.39.20250051","url":null,"abstract":"","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":" ","pages":"1-4"},"PeriodicalIF":2.2,"publicationDate":"2025-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144150484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improving <i>in vitro</i> induction efficiency of human primordial germ cell-like cells using N2B27 or NAC-based medium.","authors":"Gege Yuan, Jiachen Wang, Shuangshuang Qiu, Yunfei Zhu, Qing Cheng, Laihua Li, Jiahao Sha, Xiaoyu Yang, Yan Yuan","doi":"10.7555/JBR.38.20240433","DOIUrl":"https://doi.org/10.7555/JBR.38.20240433","url":null,"abstract":"<p><p>Primordial germ cells (PGCs), the precursors of oocytes or spermatozoa, are highly pluripotent. In recent years, the <i>in vitro</i> induction of human primordial germ cell-like cells (hPGCLCs) has advanced significantly. However, the stability and efficacy of obtaining hPGCLCs <i>in vitro</i> still require further improvement. In the current study, we identified a novel induction system by using Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12) as the basal medium supplemented with B27 and N2 (referred to as N2B27) in combination with four cytokines: bone morphogenetic protein 4 (BMP4), stem cell factor (SCF), epidermal growth factor (EGF), and leukemia inhibitory factor (LIF). The hPGCLCs induced under these conditions closely resemble PGCs from 4 to 5-week-old embryos at the transcriptome level. Compared with traditional GK15 (GMEM supplemented with 15% Knockout™ SR)-based induction conditions, the N2B27 system significantly increased the speed and efficacy of hPGCLC induction. RNA sequencing analysis revealed that this improvement resulted from an increased cell capacity to cope with hypoxic stress and avoid apoptosis. The N2B27 medium promoted an increase in mitochondrial activity, enabling cells to better cope with hypoxic stress while also reducing the production of reactive oxygen species. Moreover, by gradient concentration experiments, we demonstrated that addition of the common antioxidant N-acetyl-L-cysteine at an optimized concentration further enhanced the efficiency of PGCLC induction under GK15 conditions. Thus, our study established an optimized induction system that enhances the efficiency of hPGCLC differentiation by improving cellular resilience to hypoxic stress and apoptosis.</p>","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":" ","pages":"1-14"},"PeriodicalIF":2.2,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143970288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The association between weekly mean temperature and the epidemic of influenza across 122 countries/regions, 2014-2019.","authors":"Xiaoxiao Cao, Wenhao Zhu, Zhenghan Luo, Ran He, Yihao Li, Shirong Hui, Sheng Yang, Rongbin Yu, Peng Huang","doi":"10.7555/JBR.39.20250010","DOIUrl":"https://doi.org/10.7555/JBR.39.20250010","url":null,"abstract":"<p><p>The study examined the association between weekly mean temperature and influenza cases across 122 countries/regions (2014-2019) using a distributed lag non-linear model (DLNM). We analyzed 3145206 cases of overall influenza (Flu-All), with influenza A (Flu-A) and influenza B (Flu-B) accounting for 73.49% and 26.51%, respectively. Within a lag of 2 weeks, Flu-All incidence demonstrated a bimodal temperature relationship, with peak relative risks (RR) of 6.02 (95% CI: 1.92-20.77) at -8 ℃ and 3.08 (95% CI: 1.27-7.49) at 22 ℃. Flu-A exhibited a similar bimodal pattern, with RRs of 3.76 (95% CI: 2.39-5.91) at -8 ℃ and 2.08 (95% CI: 1.55-2.80) at 22 ℃. Flu-B demonstrated a single risk peak at 1 ℃ (RR = 4.48, 95% CI: 1.74-11.55). Subgroup analyses of climate zones revealed variations: tropical zones peaked at 12 ℃ (RR = 1.37, 95% CI: 1.08-1.74), while dry and temperate zones exhibited the highest risk at -5 ℃, with RRs of 4.49 (95% CI: 2.46-7.15) and 5.23 (95% CI: 3.17-8.64), respectively. Cold zones peaked at 1 ℃ (RR = 5.96, 95% CI: 3.76-9.43). Subgroup analyses of influenza transmission zones (ITZs) revealed variations: Africa showed higher risk between 6 ℃-14 ℃, Asia showed higher risk below 3 ℃, and Europe exhibited distinct risks of influenza peaks at -1 ℃ (Eastern), 1 ℃ (Southwest), and -20 ℃ (Northern). Elevated risks above 11 ℃ were identified in the Americas and Oceania. These findings establish a predictive framework for influenza outbreak preparedness by integrating regional temperature patterns with global climate variability.</p>","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":" ","pages":"1-10"},"PeriodicalIF":2.2,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144017990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Upregulated inwardly rectifying K ⁺ current-mediated hypoactivity of parvalbumin interneuron underlies autism-like deficits in <i>Bod1</i>-deficient mice.","authors":"Chen Li, Kerui Wang, Xingfeng Mao, Xiuxiu Liu, Yingmei Lu","doi":"10.7555/JBR.38.20240394","DOIUrl":"https://doi.org/10.7555/JBR.38.20240394","url":null,"abstract":"<p><p>Parvalbumin-positive (PV ⁺) interneuron dysfunction is believed to be linked to autism spectrum disorder (ASD), a neurodevelopmental disorder, characterized by social deficits and stereotypical behaviors. However, the underlying mechanisms of PV ⁺ interneuron dysfunction remain largely unclear. Here, we found that a deficiency of biorientation defective 1 ( <i>Bod1</i>) in PV ⁺ interneuron led to an ASD-like phenotype in <i>Pvalb-Cre</i>; <i>Bod1</i> ^<i>f/f</i> mice. Mechanistically, we identified that <i>Bod1</i> deficiency induced hypoactivity of PV ⁺ interneuron and hyperactivity of calcium/calmodulin-dependent protein kinase Ⅱ alpha (CaMKⅡα) neurons in the medial prefrontal cortex (mPFC), as determined by whole-cell patch-clamp recording. Additionally, it concurrently decreased the power of high gamma oscillation, as assessed by <i>in vivo</i> multi-channel electrophysiological recording. Furthermore, we found that <i>Bod1</i> deficiency enhanced inwardly rectifying K ⁺ current, leading to an increase in the resting membrane potential of PV ⁺ interneurons. Importantly, the gain-of-function of <i>Bod1</i> improved social deficits and stereotypical behaviors in <i>Pvalb-Cre</i>; <i>Bod1</i> ^<i>f/f</i> mice. These findings provide mechanistic insights into the PV ⁺ interneuron dysfunction and suggest new strategies for developing PV ⁺ interneuron therapies for ASD.</p>","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":" ","pages":"1-13"},"PeriodicalIF":2.2,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143752769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}