Journal of Biomedical Research最新文献

{"title":"Analysis of the efficacy of a model for auxiliary diagnosis of aortic aneurysms based on clinical pathological features.","authors":"Dian Yu, Xiaoyun Song, Yuanze Ma, Jing Tian, Yi Han","doi":"10.7555/JBR.40.20260085","DOIUrl":"https://doi.org/10.7555/JBR.40.20260085","url":null,"abstract":"<p><p>This study evaluated a multi-parameter serological model for the auxiliary diagnosis of aortic aneurysm (AA). Clinical data and serological indicators were collected from 1397 AA patients and 2000 healthy controls recruited from the Second Affiliated Hospital of Harbin Medical University (June 2021-June 2025). A comprehensive diagnostic model was developed based on serum biomarkers. Significant differences were observed between AA patients and healthy controls in blood pressure, body mass index (BMI), inflammatory markers ( <i>e.g.</i>, white blood cell count, and neutrophil count), hepatic and renal function indicators ( <i>e.g.</i>, urea, total bilirubin, and alkaline phosphatase), and nutritional parameters ( <i>e.g.</i>, albumin, total protein, and hemoglobin) ( <i>P</i> < 0.05). Multivariate analysis identified BMI, systolic blood pressure, urea, and neutrophil count as independent risk factors (OR > 1), while lymphocyte count, albumin, and serum potassium were protective factors (OR < 1). Twenty-three key variables were further selected by least absolute shrinkage and selection operator (LASSO) regression. The constructed comprehensive RiskScore showed a highly significant difference between the AA group and the control group (-16.27 ± 4.69 <i>vs.</i> -21.36 ± 1.46, <i>P</i> < 0.001), with the model demonstrating excellent discriminatory performance (concordance index [C-index] > 0.9). The results indicate that a multi-parameter serological diagnostic model distinguishes between AA patients and healthy controls, demonstrating strong discriminatory ability and potential uitility for auxiliary diagnosis of AA.</p>","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":" ","pages":"1-13"},"PeriodicalIF":2.4,"publicationDate":"2026-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148015744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial commentary on the special section on cancer research.","authors":"Editorial Board","doi":"10.7555/JBR.40.20268002","DOIUrl":"10.7555/JBR.40.20268002","url":null,"abstract":"","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":"40 3","pages":"227"},"PeriodicalIF":3.7,"publicationDate":"2026-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13231344/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148138128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrative machine learning reveals interplay between epithelial and endothelial cells elevates mixed heavy metal-related colorectal cancer risk.","authors":"Shuo Zhang, Yudan Zheng, Jing Wang, Jieyu Zhou, Zhengyi Li, Ziyan Zhou, Shenya Xu, Junyi Xin, Zan Fu, Dongying Gu, Shuwei Li, Meilin Wang","doi":"10.7555/JBR.40.20260058","DOIUrl":"https://doi.org/10.7555/JBR.40.20260058","url":null,"abstract":"<p><p>Heavy metal contamination has become an emerging global health concern, yet the promoting effects of mixed heavy metal exposure on colorectal cancer risk remains poorly understood. In this study, plasma concentrations of 25 heavy metals were obtained from an in-house elemental database comprising both colorectal cancer patients and healthy controls, and exposure concentrations were then defined based on these human plasma detection data and relevant public datasets. Machine learning algorithms and mixture exposure models were applied to identify key heavy metals associated with colorectal cancer risk. RNA-seq datasets were integrated to screen for genes exhibiting heavy metal-tumor-specific characteristics. A total of 270379 cells from 95 samples were analyzed to identify cell subsets sensitive to mixed heavy metal exposure. Molecular and cellular experiments were performed to validate the underlying mechanisms. We first identified stannum (Sn), antimony (Sb), tantalum (Ta), and thallium (Tl) as a mixture of heavy metal highly associated with colorectal cancer risk. <i>ESM1</i>, <i>SLC7A5</i> and <i>GRIA4</i> were further identified as key genes linked to colorectal tumors under conditions of mixed heavy metal exposure. Based on scRNA-seq analyses, endothelial tip cells were first identified as exhibiting heightened sensitivity to mixed heavy metal exposure. Mechanistically, acute mixed heavy metal exposure upregulated VEGFA expression in colorectal tumor epithelial cells. Co-culture experiments demonstrated enhanced epithelial-endothelial communication through the VEGFA-FLT1 axis, accompanied by increased expression of ESM1 and ANGPT2 in endothelial cells. Collectively, these findings suggest that mixed heavy metal exposure promotes angiogenesis by enhancing the interaction between colorectal tumor epithelial and endothelial cells, thereby increasing the risk of colorectal cancer. This study provides a scientific basis for improving strategies to control heavy metal contamination.</p>","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":" ","pages":"1-20"},"PeriodicalIF":2.4,"publicationDate":"2026-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148015729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of emotional and behavioral problems with Internet addiction among children and adolescents: A prospective cohort study.","authors":"Qin Wang, Wenyi Yang, Yue Zhu, Xin Wang, Chang Wang, Jia Duan, Yaming Yang, Renzhong Zhang, Jinmei Hu, Fei Wang, Jie Yang, Yuan Lin, Xiaofang Lin","doi":"10.7555/JBR.40.20260142","DOIUrl":"https://doi.org/10.7555/JBR.40.20260142","url":null,"abstract":"<p><p>The longitudinal associations between emotional and behavioral problems and Internet addiction, including how transitions between problem patterns influence risk, remain underexplored. This study investigated these associations using longitudinal data from 5123 primary and secondary school students in Jiangsu Province, China. Latent class analysis and latent transition analysis were applied to identify patterns and transitions in emotional and behavioral problems, and generalized estimating equations were used to examine their associations with subsequent Internet addiction. Emotional symptoms, conduct problems, hyperactivity/inattention, peer problems, and prosocial behavior were all significantly associated with Internet addiction. Three latent classes were identified: high difficulties, social difficulties, and low symptoms. The high difficulties group exhibited the greatest risk of Internet addiction. Compared with those maintaining stable classes, participants transitioning from the low symptoms class to the social difficulties or high difficulties classes showed elevated risks of Internet addiction (odds ratio [OR] = 2.37, 95% confidence interval [CI] = 1.58-3.55; OR = 5.62, 95% CI = 3.23-9.78). Conversely, those moving from the high difficulties class to the social difficulties or low symptoms classes had reduced risks (OR = 0.58, 95% CI = 0.36-0.94; OR = 0.28, 95% CI = 0.13-0.60). These findings suggest that both emotional and behavioral problems and their dynamic transitions are closely linked to Internet addiction risk in children and adolescents. Early identification, continuous monitoring, and timely interventions to promote positive transitions are critical for effective prevention.</p>","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":" ","pages":"1-11"},"PeriodicalIF":2.4,"publicationDate":"2026-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148015697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sestrin-2 and metabolic syndrome: Biochemical insights from a cross-sectional study.","authors":"Surbhi Tyagi, Suryakant Nagtilak, Pawan Parashar","doi":"10.7555/JBR.40.20260020","DOIUrl":"https://doi.org/10.7555/JBR.40.20260020","url":null,"abstract":"","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":" ","pages":"1-5"},"PeriodicalIF":2.4,"publicationDate":"2026-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148015687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"GPC6 facilitates progression of SHH-subgroup medulloblastoma by enhancing Hedgehog secretion and signaling responses.","authors":"Yue Wang, Qingyue Meng, Qin Zhu, Xinyi Zhang, Xinfa Wang, Junping He, Jing Cai, Xiaohong Pu, Zihe Ai, Qinya Li, Kedui Pu, Tingting Yu, Chen Liu, Shen Yue","doi":"10.7555/JBR.39.20250406","DOIUrl":"10.7555/JBR.39.20250406","url":null,"abstract":"<p><p>Medulloblastoma (MB) is the most common malignant tumor of the cerebellum in children. The Sonic Hedgehog (SHH) subgroup of MB (SHH-MB) is driven by aberrant activation of the SHH pathway; however, mutations in genes related to this pathway are relatively rare, posing challenges for therapeutic development. Glypican-6 (GPC6), a heparan sulfate proteoglycan, is highly expressed in SHH-MB. In this study, we demonstrate the synchronous expression of GPC6 with GLI family zinc finger 1 (GLI1) in both the developing cerebellum and medulloblastoma. GPC6 promotes cell proliferation, migration, and invasion in SHH-MB cell lines (DAOY and ONS-76). Consistently, GPC6 enhances the SHH pathway activity by upregulating GLI1 expression, supports ciliogenesis essential for signal transduction, and facilitates SHH ligand expression <i>via</i> extracellular vesicles. These findings suggest that GPC6 acts as a key regulator of SHH signaling and represents a potential therapeutic target in SHH-MB.</p>","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":" ","pages":"228-246"},"PeriodicalIF":3.7,"publicationDate":"2026-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13231367/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145648572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic effects of nicotinamide mononucleotide and Indian gooseberry in oxidative stress and inflammation-induced damage on human retinal pigment epithelial cells: A pilot study.","authors":"Deokho Lee, Jolly Shamsun Nahar, Sichan Kim, Soon Sung Lim","doi":"10.7555/JBR.39.20250452","DOIUrl":"10.7555/JBR.39.20250452","url":null,"abstract":"","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":" ","pages":"327-331"},"PeriodicalIF":3.7,"publicationDate":"2026-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13231348/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146085883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrating bulk and single-cell sequencing reveals cellular heterogeneity between lung adenocarcinoma in smokers and never-smokers.","authors":"Zihuan Zhao, Pan Yang, Yuhan Liu, Kai Wang, Xianfeng Xu, Yuzhuo Wang, Meng Zhu, Na Qin, Cheng Wang, Weimin Li, Hongxia Ma, Zhoufeng Wang, Hongbing Shen","doi":"10.7555/JBR.39.20250160","DOIUrl":"10.7555/JBR.39.20250160","url":null,"abstract":"<p><p>Lung cancer in smokers (LCIS) and lung cancer in never-smokers (LCINS) are different entities with distinct molecular features. However, their cellular heterogeneity still requires further investigation. Through an integrated analysis of single-cell RNA sequencing and bulk sequencing data, we identified cell subpopulations associated with smoking and non-smoking patients. Subsequent transcriptomic analyses were performed to elucidate differences in cellular functions and the tumor microenvironment. We observed that smoking-associated cancer cells exhibited a higher degree of aggressiveness, which may correlate with an adverse prognosis in smoking patients. Additionally, immunosuppressive <i>CXCL10</i> ⁺ macrophages may contribute to tumorigenesis in smokers, and the immunoregulatory LGALS9-HAVCR2 axis could be a potential immunotherapeutic target. In non-smokers, the inflammatory microenvironment may be involved in tumor development. Moreover, the reduced anti-tumor cytotoxicity may be associated with their suboptimal immunotherapeutic response. Our study uncovered differences in oncogenic and immune escape mechanisms between LCIS and LCINS patients and suggests potential immunotherapeutic strategies.</p>","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":" ","pages":"247-265"},"PeriodicalIF":3.7,"publicationDate":"2026-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13231364/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144955231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of stem cells in ocular diseases: Progress and challenges.","authors":"Tongyao Fu, Yan Ma, Yifei Jiang, Chang Jiang, Xiumiao Li, Qin Jiang","doi":"10.7555/JBR.39.20250251","DOIUrl":"10.7555/JBR.39.20250251","url":null,"abstract":"<p><p>Ocular diseases, including corneal disease, glaucoma, age-related macular degeneration (AMD), diabetic retinopathy, and retinopathy of prematurity, can significantly impair vision and reduce quality of life. Because degenerated cells in these diseases are unable to regenerate, treatments for these conditions have limited efficacy. Stem cell therapies are revolutionizing the treatment of degenerative eye conditions, enabling structural and functional restoration through mechanisms such as cell replacement and paracrine signaling. This review examines advances in stem cell therapy for ocular diseases, from preclinical studies to early clinical trials, focusing on various types of stem cells, including embryonic stem cells, induced pluripotent stem cells (iPSCs), and mesenchymal stem cells. Significant progress has been made with iPSC-derived retinal pigment epithelial cell transplantation in AMD treatment, showing cell survival in trials, and with mesenchymal stem cells for corneal repair through anti-inflammatory effects. Challenges remain, such as controlling differentiation to prevent tumorigenesis, managing immune rejection, and ensuring manufacturing processes that comply with Good Manufacturing Practice standards. By integrating mechanistic insights with translational strategies, this review outlines pathways to optimize stem cell therapies for previously intractable ocular diseases.</p>","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":" ","pages":"291-311"},"PeriodicalIF":3.7,"publicationDate":"2026-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13231361/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145075309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Where do I submit my next paper? A practical guide for biomedical researchers.","authors":"Mohammad S Alrashdan","doi":"10.7555/JBR.39.20250147","DOIUrl":"10.7555/JBR.39.20250147","url":null,"abstract":"","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":" ","pages":"341-344"},"PeriodicalIF":3.7,"publicationDate":"2026-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13231345/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145033230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}