Journal of Biomedical Research最新文献_第4页

Associations of diet with infectious diseases in UK Biobank. 英国生物数据库中饮食与传染病的关系。

IF 2.2 4区医学

Journal of Biomedical Research Pub Date : 2024-05-25 DOI: 10.7555/JBR.37.20230319

Junlan Tu, Xuehong Cai, Yifan Wang, Xiangyu Ye, Meijie Yu, Sheng Yang, Rongbin Yu, Peng Huang

{"title":"Associations of diet with infectious diseases in UK Biobank.","authors":"Junlan Tu, Xuehong Cai, Yifan Wang, Xiangyu Ye, Meijie Yu, Sheng Yang, Rongbin Yu, Peng Huang","doi":"10.7555/JBR.37.20230319","DOIUrl":"10.7555/JBR.37.20230319","url":null,"abstract":"This research aims to utilize multivariate logistic regression to explore associations between the frequency of 13 food groups intake (or four diet groups) and infectious diseases. 487849 participants from the UK Biobank were enrolled, and 75209 participants were diagnosed with infectious diseases. Participants reporting the highest intake frequency of processed meat [odds ratio ( OR) = 1.0964; 95% CI: 1.0622-1.1318] and red meat ( OR = 1.0895; 95% CI: 1.0563-1.1239) had a higher risk of infectious diseases compared to those with the lowest intake frequency. Consuming fish 2.0-2.9 times ( OR = 0.8221; 95% CI: 0.7955-0.8496), cheese ≥5.0 times ( OR = 0.8822; 95% CI: 0.8559-0.9092), fruit 3.0-3.9 servings ( OR = 0.8867; 95% CI: 0.8661-0.9078), and vegetables 2.0-2.9 servings ( OR = 0.9372; 95% CI: 0.9189-0.9559) per week were associated with a lower risk of infection. Low meat-eaters ( OR = 0.9404; 95% CI: 0.9243-0.9567), fish-eaters ( OR = 0.8391; 95% CI: 0.7887-0.8919), and vegetarians ( OR = 0.9154; 95% CI: 0.8561-0.9778) had a lower risk of infectious diseases compared to regular meat-eaters. Mediation analysis was performed, revealing glycosylated hemoglobin, white blood cell counts, and body mass index were mediators in the relationships between diet groups and infectious diseases. This study suggested that intake frequency of food groups is a factor in infectious diseases and fish-eaters have a lower risk of infection.","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":" ","pages":"1-15"},"PeriodicalIF":2.2,"publicationDate":"2024-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11629163/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141161439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

GSDMD protects intestinal epithelial cells against bacterial infections through its N-terminal activity impacting intestinal immune homeostasis. GSDMD 通过其 N 端活性影响肠道免疫平衡，保护肠道上皮细胞免受细菌感染。

IF 2.2 4区医学

Journal of Biomedical Research Pub Date : 2024-05-25 DOI: 10.7555/JBR.38.20240041

Honghui Li, Jie Pu, Dongxue Yang, Lu Liu, Yingchao Hu, Shuo Yang, Bingwei Wang

{"title":"GSDMD protects intestinal epithelial cells against bacterial infections through its N-terminal activity impacting intestinal immune homeostasis.","authors":"Honghui Li, Jie Pu, Dongxue Yang, Lu Liu, Yingchao Hu, Shuo Yang, Bingwei Wang","doi":"10.7555/JBR.38.20240041","DOIUrl":"10.7555/JBR.38.20240041","url":null,"abstract":"The intestinal mucosal barrier serves as a vital guardian for gut health, maintaining a delicate equilibrium between gut microbiota and host immune homeostasis. Recent studies have found the intricate roles of Gasdermin D (GSDMD), a key executioner of pyroptosis downstream of the inflammasome, within the intestine, including controlling colitis in intestinal macrophage and the regulatory function in goblet cell mucus secretion. Thus, the exact role and nature of GSDMD's regulatory function in maintaining intestinal immune homeostasis and defending against pathogens remain elucidation. Here, we uncover that GSDMD plays a key role in defending against intestinal Citrobacter rodentium infection, with high expression in intestinal epithelial and lamina propria myeloid cells. Our results show that GSDMD specifically acts in intestinal epithelial cells to fight the infection, independently of its effects on antimicrobial peptides or mucin secretion. Instead, the resistance is mediated through GSDMD's N-terminal fragments, highlighting its importance in intestinal immunity. However, the specific underlying mechanism of GSDMD N-terminal activity in protection against intestinal bacterial infections still needs further study to clarify in the future.","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":" ","pages":"1-12"},"PeriodicalIF":2.2,"publicationDate":"2024-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11629157/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141161522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel anti-inflammatory peptide alleviates liver ischemia-reperfusion injury. 新型抗炎肽可减轻肝脏缺血再灌注损伤

IF 2.3 4区医学

Journal of Biomedical Research Pub Date : 2024-05-25 DOI: 10.7555/JBR.38.20240020

Xuejun Xu, Kaineng Sun, Hao Chang, Chunxiang Shen, Xiangdong Li, Yangyue Ni, Yuxiao Zhu, Huiquan Wang, Ruiyan Xiong, Jon Rob Padde, Zhipeng Xu, Lin Chen, Lu Chen, Min Hou, Liyong Pu, Minjun Ji

{"title":"Novel anti-inflammatory peptide alleviates liver ischemia-reperfusion injury.","authors":"Xuejun Xu, Kaineng Sun, Hao Chang, Chunxiang Shen, Xiangdong Li, Yangyue Ni, Yuxiao Zhu, Huiquan Wang, Ruiyan Xiong, Jon Rob Padde, Zhipeng Xu, Lin Chen, Lu Chen, Min Hou, Liyong Pu, Minjun Ji","doi":"10.7555/JBR.38.20240020","DOIUrl":"https://doi.org/10.7555/JBR.38.20240020","url":null,"abstract":"Ischemia-reperfusion injury (IRI) remains inevitable in liver surgeries, macrophages play a critical role in the development of IRI, but little is known about the macrophages regulate pathogenesis of IRI. Based on target-guided screening, we identified a small 3 kDa peptide (SjDX5-271) from various schistosome egg-derived peptides that induced M2 macrophage polarization. SjDX5-271 treatment protected the mice against liver IRI through promoting M2 macrophage polarization, the protective effect was abrogated when the macrophages were depleted. Transcriptomic sequencing showed that the TLR signaling pathway was significantly inhibited in macrophages derived from the SjDX5-271 treatment group. We further identified that SjDX5-271 promotes M2 macrophage polarization by inhibiting the TLR4/MyD88/NF-κB signaling pathway and further alleviates hepatic inflammation in liver IRI. Collectively, SjDX5-271 exhibits promising therapeutic effects in IRI and represents a novel therapeutic approach for IRI, even in immune-related diseases. This study revealed the development of a new biologic from the parasite and enhanced our understanding of host-parasite interplay, providing a blueprint for future therapies for immune-related diseases.","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":" ","pages":"1-15"},"PeriodicalIF":2.3,"publicationDate":"2024-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141161647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression profiling and bioinformatics analysis of serum exosomal circular RNAs in lymph node metastasis of papillary thyroid carcinoma. 甲状腺乳头状癌淋巴结转移中血清外泌体环状RNA的表达谱分析和生物信息学分析

IF 2.3 4区医学

Journal of Biomedical Research Pub Date : 2024-05-25 DOI: 10.7555/JBR.37.20230304

Huiyong Peng, Zhangwei Zhu, Jie Xing, Qian Xu, Changfeng Man, Shengjun Wang, Yingzhao Liu, Zhengdong Zhang

{"title":"Expression profiling and bioinformatics analysis of serum exosomal circular RNAs in lymph node metastasis of papillary thyroid carcinoma.","authors":"Huiyong Peng, Zhangwei Zhu, Jie Xing, Qian Xu, Changfeng Man, Shengjun Wang, Yingzhao Liu, Zhengdong Zhang","doi":"10.7555/JBR.37.20230304","DOIUrl":"https://doi.org/10.7555/JBR.37.20230304","url":null,"abstract":"Most papillary thyroid carcinoma (PTC) patients have a good prognosis, but lymph node metastasis (LNM) is the most common progressive manifestation and often leads to a poor-prognosis. However, few studies focused on the underlying mechanisms of LNM. This study aimed to identity the potential role of exosomal circRNAs that contribute to LNM in PTC. We found that 9000 aberrantly expressed exosomal circRNAs in PTC patients with LNM, including 684 observably upregulation and 2193 notably downregulation. Functional enrichment analyses indicated that these aberrantly expressed circRNAs were mainly enriched in a variety of molecules and signaling pathways related to the progression and LNM of PTC. Bioinformatics analysis screened 14 circRNA-miRNA-mRNA networks associated with LNM-related signaling pathways in PTC. Moreover, circTACC2-miR-7-EGFR and circBIRC6-miR-24-3p-BCL2L11 axes were verified for potential involvement in PTC with LNM. Additionally, 4 upregulated circRNAs-related hub genes and 8 hub genes associated with downregulated circRNAs were screened, some of which were involved in LNM of PTC through verification. Collectively, our data provided a novel framework for in-depth investigation of the function of dysregulated exosomal circRNAs and their potential biomarkers in PTC patients with LNM.","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":" ","pages":"1-15"},"PeriodicalIF":2.3,"publicationDate":"2024-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141175361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association between exposure to per- and polyfluoroalkyl substance and liver injury in American adults. 美国成年人接触全氟和多氟烷基物质与肝损伤之间的关系。

IF 2.2 4区医学

Journal of Biomedical Research Pub Date : 2024-05-25 DOI: 10.7555/JBR.38.20240018

Yuqian Yan, Lu Zhang, Xin Xu, Jing Lu, Xinyuan Ge, Maojie Liu, Juan Yang, Chan Tian, Zijun Ge, Chengxiao Yu, Wen Guo, Chunyan Ye, Qun Zhang

{"title":"Association between exposure to per- and polyfluoroalkyl substance and liver injury in American adults.","authors":"Yuqian Yan, Lu Zhang, Xin Xu, Jing Lu, Xinyuan Ge, Maojie Liu, Juan Yang, Chan Tian, Zijun Ge, Chengxiao Yu, Wen Guo, Chunyan Ye, Qun Zhang","doi":"10.7555/JBR.38.20240018","DOIUrl":"10.7555/JBR.38.20240018","url":null,"abstract":"Epidemiological data is scarce regarding the association between exposure to mixtures of per- and polyfluoroalkyl substances (PFASs) and liver injury in the general populace. The current research used data from the National Health and Nutrition Examination Survey (2009-2018). The PFAS exposure levels were defined by the serum concentrations of PFASs with > 70% detection in samples, namely perfluorooctanoic acid (PFOA), perfluorononanoic acid (PFNA), perfluorohexane sulfonic acid (PFHxS), perfluorodecanoic acid (PFDeA), and perfluorooctane sulfonic acid (PFOS). Liver injury was assessed from two aspects: first, the degree of liver inflammation was determined based on serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), glutamyltransferase (GGT), and total bilirubin (TBIL) levels; second, the degree of liver fibrosis was determined based on fibrosis-4 (FIB-4) index. We assessed the associations between individual or total PFAS exposure and these outcomes using multivariable linear regression models and logistic regression models, restricted cubic splines, and weighted quantile sum regression. Among the samples of 7484 American adults, the median concentration of PFOS was the highest, followed by PFOA and PFHxS. Using multivariable linear regression, a positive correlation was observed between all PFASs and liver enzymes such as ALT, AST, and TBIL. Additionally, the weighted quantile sum model indicated an overall positive association between the five PFASs and liver injury indicators. For liver function biomarkers and liver fibrosis, PFNA and PFOS were the most heavily weighting chemicals, respectively. Our findings provide new epidemiological evidence indicating a potential association between PFAS exposure and adverse effects on liver injury biomarkers, highlighting the potentially harmful effects of PFAS exposure on liver health.","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":" ","pages":"1-12"},"PeriodicalIF":2.2,"publicationDate":"2024-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11629162/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141161436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Systemic lupus erythematosus therapeutic strategy: From immunotherapy to gut microbiota modulation. 系统性红斑狼疮的治疗策略：从免疫疗法到肠道微生物群调节。

IF 2.2 4区医学

Journal of Biomedical Research Pub Date : 2024-05-25 DOI: 10.7555/JBR.38.20240009

Vitaly Chasov, Ekaterina Zmievskaya, Irina Ganeeva, Elvina Gilyazova, Damir Davletshin, Maria Filimonova, Aygul Valiullina, Anna Kudriaeva, Emil Bulatov

{"title":"Systemic lupus erythematosus therapeutic strategy: From immunotherapy to gut microbiota modulation.","authors":"Vitaly Chasov, Ekaterina Zmievskaya, Irina Ganeeva, Elvina Gilyazova, Damir Davletshin, Maria Filimonova, Aygul Valiullina, Anna Kudriaeva, Emil Bulatov","doi":"10.7555/JBR.38.20240009","DOIUrl":"10.7555/JBR.38.20240009","url":null,"abstract":"Systemic lupus erythematosus (SLE) is characterized by a systemic dysfunction of the innate and adaptive immune systems, leading to an attack on healthy tissues of the body. During the development of SLE, pathogenic features, such as the formation of autoantibodies to self-nuclear antigens, caused tissue damage including necrosis and fibrosis, with an increased expression of type Ⅰ interferon (IFN) regulated genes. Treatment of lupus with immunosuppressants and glucocorticoids, which are used as the standard therapy, is not effective enough and causes side effects. As an alternative, more effective immunotherapies have been developed, including monoclonal and bispecific antibodies that target B cells, T cells, co-stimulatory molecules, cytokines or their receptors, and signaling molecules. Encouraging results have been observed in clinical trials with some of these therapies. Furthermore, a chimeric antigen receptor T cells (CAR-T) therapy has emerged as the most effective, safe, and promising treatment option for SLE, as demonstrated by successful pilot studies. Additionally, emerging evidence suggests that gut microbiota dysbiosis may play a significant role in the severity of SLE, and the use of methods to normalize the gut microbiota, particularly fecal microbiota transplantation (FMT), opens up new opportunities for effective treatment of SLE.","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":" ","pages":"1-16"},"PeriodicalIF":2.2,"publicationDate":"2024-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11629155/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141199215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anesthetic dilemmas in an achondroplastic patient undergoing elective cesarean section. 一名接受择期剖腹产手术的软骨发育不全患者的麻醉困境。

IF 2.2 4区医学

Journal of Biomedical Research Pub Date : 2024-05-25 DOI: 10.7555/JBR.37.20230301

Aaron Brown, Hong Liu, Cristina Chandler

引用次数: 0

DEC1 deficiency protects against bone loss induced by ovariectomy through inhibiting inflammation. DEC1 缺乏症可通过抑制炎症防止卵巢切除术引起的骨质流失。

IF 2.2 4区医学

Journal of Biomedical Research Pub Date : 2024-05-25 DOI: 10.7555/JBR.38.20240069

Lan Lin, Zhiyi Qiang, Kaiao Chen, Ying Huo, Wei Liu, Jian Yang

{"title":"DEC1 deficiency protects against bone loss induced by ovariectomy through inhibiting inflammation.","authors":"Lan Lin, Zhiyi Qiang, Kaiao Chen, Ying Huo, Wei Liu, Jian Yang","doi":"10.7555/JBR.38.20240069","DOIUrl":"10.7555/JBR.38.20240069","url":null,"abstract":"Previous studies have shown that differentiated embryo-chondrocyte expressed gene 1 (DEC1) promotes osteoblast osteogenesis. To investigate the role of DEC1 in postmenopausal osteoporosis (PMOP), we utilized the two types (DEC1 +/+, DEC1 -/-) mice to establish an ovariectomy (OVX) model and found that the bone loss in DEC1 -/- OVX mice were much less than that in DEC1 +/+ OVX mice. The expression levels of RUNX2 and OSX significantly increased in DEC1 -/- OVX mice compared with those in DEC1 +/+ OVX mice. Whereas, NFATc1, c-Fos, CTSK and RANKL/OPG significantly decreased in DEC1 -/- OVX mice compared with those in DEC1 +/+ OVX mice. Likewise, DEC1 deficiency suppressed IL-6 and IL-1β. Further study showed Runx2, Osx, Alp, and Ocn significantly increased in DEC1 -/- OVX BMSCs compared with those in DEC1 +/+ OVX BMSCs. And the mRNA levels of IL-1β, IL-6, Tnf-α and Ifn-γ increased significantly in DEC1 +/+ OVX BMMs compared with those in DEC1 +/+ sham BMMs, but not in DEC1 -/- OVX BMMs compared with those in DEC1 -/- sham BMMs. Furthermore, the p-IκBα and p-P65 significantly increased in DEC1 +/+ OVX BMMs compared with those in DEC1 +/+ sham BMMs, but did not increase in DEC1 -/- OVX BMMs compared with those in DEC1 -/- sham BMMs. Taken together, DEC1 deficiency inhibits the NF-κB pathway induced by OVX, thereby decreasing cytokines, and subsequently, inhibits the decrease of osteogenesis and the increase of osteoclastogenesis caused by OVX. The findings provide a novel understanding of postmenopausal osteoporosis development, which offers potential avenues for the intervention strategies.","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":" ","pages":"1-16"},"PeriodicalIF":2.2,"publicationDate":"2024-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11629160/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141161374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Post-transcriptional dysregulation in autism, schizophrenia, and bipolar disorder. 自闭症、精神分裂症和躁狂症的转录后失调。

IF 2.3 4区医学

Journal of Biomedical Research Pub Date : 2024-05-25 DOI: 10.7555/JBR.38.20240114

Yuanyuan Wang, Yitong Yan, Bin Zhou, Mingyan Lin

{"title":"Post-transcriptional dysregulation in autism, schizophrenia, and bipolar disorder.","authors":"Yuanyuan Wang, Yitong Yan, Bin Zhou, Mingyan Lin","doi":"10.7555/JBR.38.20240114","DOIUrl":"https://doi.org/10.7555/JBR.38.20240114","url":null,"abstract":"The alteration of gene expression is not restricted to transcriptional regulation but includes a variety of post-transcriptional mechanisms, however, the role of the latter underlying many diseases remains relatively unknown. By utilizing an RNA-Seq dataset of 1510 brain samples from individuals with autism spectrum disorder (ASD), bipolar disorder (BD), schizophrenia (SCZ), and controls, we assessed the contribution of post-transcriptional dysregulation and identified top perturbators accountable for transcriptomic changes of expression in neuropsychiatric disorders. Around 30% of the variability in expression can be attributed to post-transcriptional dysregulation. Interestingly, RNA stability tended to decrease in SCZ and BD, leading to the inhibition of neurogenesis and neural differentiation, while the increase in ASD, resulted in enhanced activity of apoptosis. This finding implicated contrasting pathologies involving RNA stability among neuropsychiatric disorders. An RNA binding protein (RBP)-ELAVL3 - is predicted to be significantly involved in the disruption of RNA stability in all three disorders. To validate, we knocked down its expression in cerebral organoids. Not only differentially expressed genes in ELAVL3-knockdown covered a considerable proportion of predicted targets in three disorders, we also found neurogenesis was significantly affected, given the diminished proliferation and consequently the reduced size of the organoids. Our study extends the current understanding of the link between post-transcriptional regulation and neuropsychiatric disorders and provides new therapeutic targets for early intervention.","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":" ","pages":"1-14"},"PeriodicalIF":2.3,"publicationDate":"2024-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141161684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of SRPK1-mediated phosphorylation of SR proteins in the chromatin configuration transition of mouse germinal vesicle oocytes. SRPK1 介导的 SR 蛋白磷酸化在小鼠生殖泡卵母细胞染色质构型转变中的作用

IF 2.3 4区医学

Journal of Biomedical Research Pub Date : 2024-05-25 DOI: 10.7555/JBR.38.20240054

Xia Wang, Shuai Zhou, Haojie Yin, Jian Han, Yue Hu, Siqi Wang, Congjing Wang, Jie Huang, Junqiang Zhang, Xiufeng Ling, Ran Huo

{"title":"The role of SRPK1-mediated phosphorylation of SR proteins in the chromatin configuration transition of mouse germinal vesicle oocytes.","authors":"Xia Wang, Shuai Zhou, Haojie Yin, Jian Han, Yue Hu, Siqi Wang, Congjing Wang, Jie Huang, Junqiang Zhang, Xiufeng Ling, Ran Huo","doi":"10.7555/JBR.38.20240054","DOIUrl":"https://doi.org/10.7555/JBR.38.20240054","url":null,"abstract":"Meiotic resumption in mammalian oocytes involves nucleus and organelle structural changes, notably chromatin configuration transitioning from non-surrounding nucleolus (NSN) to surrounding nucleolus (SN) in germinal vesicle (GV) oocytes. Our study found that nuclear speckles, a subnuclear structure mainly composed of serine-arginine (SR) proteins, changed from a diffuse spotted distribution in mouse NSN oocytes to an aggregation pattern in SN oocytes. We further discovered that SRPK1, an enzyme phosphorylating SR proteins, co-localized with NS at SN stage and NSN oocytes failed to convert into SN oocytes after inhibiting the activity of SRPK1. Furthermore, the typical structure of chromatin ring around the nucleolus in SN oocytes collapsed after inhibitor treatment. To explore the underlying mechanism, phosphorylated SR proteins were confirmed to be associated with chromatin by salt extraction experiment, and in situ DNase I assay showed that the accessibility of chromatin enhanced in SN oocytes with SRPK1 inhibited, accompanied by decreased repressive modification on histone and abnormal recurrence of transcriptional signal. In conclusion, our results indicated that SRPK1-regulated phosphorylation on SR proteins was involved in the NSN to SN transition and played an important role in maintaining the condensation nucleus of SN oocytes via interacting with chromatin.","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":" ","pages":"1-11"},"PeriodicalIF":2.3,"publicationDate":"2024-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141161696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0